Problem Solver
Mary Johansson
Areas Mary Johansson is Knowledgeable in:
Fluorescent probes, bioconjugate chemistry, oligonucleotide synthesis, genomic assays, analytical insturmentation, photophysics, physical organic chemistry, electron/energy transfer, biological sensors.
Techniques Mary Johansson Uses:
-I read the scientific literature to get ideas and background information. Sometimes I set a problem aside for a while to get a fresh perspective. Drawing pictures, diagrams, flow charts, building models - all can help, depending on the problem.
Mary Johansson's Problem Solving Skills:
- synthesis and spectroscopy of oligosilanes
- Electron and energy transfer
- Fluorescence assays, FRET
- Genomic assays and PCR
- solar cells - research and development
- Spectroscopy - absorbance, fluorescence, NMR
- Oligonucleotide synthesis and conjugation
- Biotech research and development
- bioconjugate chemistry
- adjunct professor teaching chemistry
- lanthanide and metalocomplex luminescence
Mary Johansson's Problem Solving Experience:
- -I designed and built several pieces of equipment during my Ph D including a variable temperature flow cell with a quartz cuvette.
- -I discovered a new quenching mechanism that occurs in dual-labeled labeled probes. This alternative mechanism allows for very high signal/noise ratios in pro-fluorescent assays. This lead to three publications, including one in J Am Chem Soc.
- - I wrote a grant proposal for time-gated luminescent reporters and got a 700 K USD SBIR grant. I figured out how to conjugate the lanthanide complexes to oligonucleotides and ran several assays. This lead to a paper in J Am Chem Soc. The technology worked but was too expensive.
- -I developed a new fluorescent dye to label oligonucleotides leading to a patent. I worked out the manufacturing protocol, QC criteria, applications development, marketing and customer research. This is now a successful product generating >50K USD in sales.