Sergei Borukhov

Areas Sergei Borukhov is Knowledgeable in:

Sergei Borukhov's Problem Solving Skills:

1. Developement of assays for protein domain organization, structural integrity and enzymatic activity
2. Reconstitution and assembly of multiprotein complexes
3. Analysis of molecular mechanisms of transcription and its regulation
4. Development of methods to investigate protein-protein and protein-nucleic acid interactions
5. Protein crystallization for X-ray structural analysis
6. Development of methods for protein/peptide isolation and purification

Sergei Borukhov's Problem Solving Experience:

1. I developed methods for photocrosslinking between RNA/DNA and proteins of transcriptional machinery, and between RNA polymerase and various transcription factors and regulators. I designed and verified approaches to map and identify sites of interaction in proteins and DNA/RNA.
2. I developed protocols for purification of mammalian, yeast and bacterial natural and recombinant proteins such as: interferons, insulin; alpha-amylase, proteinases, RNA polymerases and transcription factors
3. I developed approaches to decipher the molecular mechanisms of action of several transcription regulators using a combination of genetic, biochemical and biophysical methods