Problem Solver
Ali Rafati
Areas Ali Rafati is Knowledgeable in:
1. Genetic engineering and manipulation of organisms.
2. Gene expression and regulation.
3. DNA sequencing and analysis.
4. Genetic diseases and disorders.
5. Molecular biology techniques.
6. Biotechnological applications in medicine, agriculture, and industry.
7. Bioinformatics and computational biology.
2. Gene expression and regulation.
3. DNA sequencing and analysis.
4. Genetic diseases and disorders.
5. Molecular biology techniques.
6. Biotechnological applications in medicine, agriculture, and industry.
7. Bioinformatics and computational biology.
Ali Rafati's Problem Solving Experience:
- 1. Denaturation: The DNA sample is heated to around 95°C to separate the double-stranded DNA into single strands.
2. Annealing: The sample is cooled to around 50-65°C, allowing the primers to bind to the specific DNA sequences flanking the target region.
3. Extension: The temperature is raised to around 72°C, and a heat-stable DNA polymerase enzyme synthesizes new DNA strands complementary to the single-stranded DNA templates, using the primers as starting points.
4. Repeat: Steps 1-3 are repeated for multiple cycles (usually 20-40), resulting in exponential amplification of the target DNA sequence.