Kamila Nejedla

Areas Kamila Nejedla is Knowledgeable in:

Techniques Kamila Nejedla Uses:

Kamila Nejedla's Problem Solving Skills:

1. strategic marketing plan development
2. generating epidemiology models & market forecasts for licencing/tech evaluation
3. genetic engineering of hybridoma cell line for monoclonal antibody production
4. marketing - gastric band for weight loss surgery (Johnson & Johnson)
5. quantitative PCR for diagnostics and gene therapy evaluation (Johnson & Johnson, SpeeDx Pty Ltd)
6. events management
7. literature search, analysis, consolidation for business cases/market evaluation/reports/presentation

Kamila Nejedla's Problem Solving Experience:

1. I designed a relational database of epidemiology data from 13 Asia Pacific countries to forecast market size of potential licencing opportunities in the region (Johnson & Johnson)
2. I developed a reproducible method of transferring whole chromosomes into Burkitts Lymphoma cell line, a notoriously sensitive line to manipulation. Solution: design a gentler method of conventional cell fusion excluding centrifugation as driver of fusion - immobilize cells onto tissue culture flask surface (without cell activation), use gravity to layer fusion partner (microcells) on top, grow directly from flask surface.
3. I developed a method of rational design to develop a robust qPCR assay for testing patient samples in HIV clinical trial (Johnson & Johnson). The problem arose from the inter-well variability in a 96-well plate, resulting in inability to validate assay to clinical trial standards. Solution: add same amount of DNA in all wells across multiple plates, assess quantification and reproducibility of measurements in each well, sort wells into groups that measured "high", "medium"and "low" vs amount added, randomly assigned triplicates to each group, then retest over quantitative range.
4. I developed a method of creating HAT-sensitive cells lines using UV instead of conventional chemical treatment to meet deadline (said chemical was on backorder)