Problem Solver
Margarita Todorova
Areas Margarita Todorova is Knowledgeable in:
clinical drug development and testing on cells and other models, genetic testing and gene expression/silencing, cellular proteins, structure and activity. Food science and nutrition,
Techniques Margarita Todorova Uses:
I used imaging modalities, statistical tests, excel, GraphPad Prism, Clone Manager, primer design
Margarita Todorova's Problem Solving Skills:
- Fluorescence staining, slide preparation and florescent microscopy analysis
- Developed enzyme assays and studied DNA damage and DNA methylation activity in plants
- cell imaging, immunology and stem cells by FACS analysis
- preclinical evaluation of vascular disrupting effect of ultrasound in combinations with clinical approved dugs
- microbiological assays for cell culture sterility, microplasma contamination tests
- diagnostic tests for monitoring drug response to anti-cancer therapy
- viability assays to study the effect of drugs in tissue culture
- qPCR for gene expression analysis in cancer cells
- performed protein analysis as biomarker study and cell signaling mechanisms
- statistical data analysis, including time-to-event data in tumor growth delay
- performed analytical, microbiological, biochemical tests on product in the food industry
Margarita Todorova's Problem Solving Experience:
- I designed and optimized primers to quantify gene expression in normal versus treated tissue, normal versus cancer cells
- I employed ELISA, western blots, reporter gene constructs cloning and expression, RT-PCR, DNA damage, FACS analysis to study cellular responses to anticancer drugs in cells and tumor tissues
- I developed immunoassay and cells staining technique and used fluorescence microscopy to study receptors and intracellular proteins interactions
- I modified comet DNA damage assay and performed time-dependent quantitative analysis on several cancer cells lines after drug treatment.