Problem Solver
Michael Thelen
Areas Michael Thelen is Knowledgeable in:
Genome-enabled biological research; human DNA damage disorders; plant cell biology and cell wall analysis; protein expression and purification; clone library construction and functional screening
Techniques Michael Thelen Uses:
Literature and patent research; discussion with colleagues
Michael Thelen's Problem Solving Skills:
- Biochemistry, microscopy and cell biology of plant cell wall biosynthesis and degradation
- Animal, plant and bacterial cell culture
- Clone library construction and functional screening
- Biochemical and proteomic analyses of environmental microbial communities
- Complementation of mutant phenotypes using molecular genetics
- Degradation of plant biomass using microbial enzymes
- Phylogenetic analysis of bacteria in complex communities
- Literature review, manuscript writing, publishing papers
Michael Thelen's Problem Solving Experience:
- I helped develop a method to engineer bacteria to tolerate chemicals encountered in biofuel processing.
- I designed an assay to determine the function of an enzyme in DNA recombination, using specific DNA substrates.
- I helped develop a computational approach to linking a protein's function to its molecular structure.
- I developed a quantitative method for monitoring cellulose degradation in single plant cells.
- I developed a method for purification of proteins produced by in bacteria by recombinant DNA techniques.