Problem Solver
Nima Ghahremani nezhad
Areas Nima Ghahremani nezhad is Knowledgeable in:
Cloning, co-transformation, co-expression, especially once the protein must be express in the soluble form.
Nima Ghahremani nezhad's Problem Solving Skills:
- Molecular biology, genetic engineering and enzymology
Nima Ghahremani nezhad's Problem Solving Experience:
- I improved and optimized the protocols for simultaneous co-transformation of two plasmids in E.coli host that is time- and chemical-saving.
I improved the protocol for two-steps purification, once the second step is related to removing His-tag purification and ion exchange chromatography is not available. Then, both steps can be carried out using affinity chromatogarphy.