Innovative People

Jose Alvelo

AREAS OF EXPERTISE
Over 6 years of all-source military intelligence experience, to include collection and analysis of
intelligence related to chemical/biological weapons, terrorism, and weapons of mass destruction in
support of Special Forces and Rapid-Deployment missions. More than 9 years of research experience in
various biological fields including; molecular and cell biology, microbiology, immunology, biochemistry,
and biotechnology in academia and military research programs. Over 3 years of teaching experience in
anatomy & physiology, Spanish, and English as a second language (ESL). Seven years of Science &
Technology Program Management for chemical and biological warfare defense for the Department of
defense.
Biodefense: Evaluation of cutting-edge biosensors technologies and development of molecular assays for
rapid identification of biological warfare agents from environmental samples. Provide program
management support to the Joint Science & Technology Office for Chemical and Biological Defense and
SME support to other DTRA organizations.
Nanoscience-Nanotechnology: Provide program management support to the Joint Science &
Technology Office for Chemical and Biological Defense Nanotechnology Initiative. Responsible for
selecting research projects for funding in support of CB defense applications.
Molecular Biology: Bacterial plasmid isolation, gene cloning and recombinant protein expression, gene
sequence analysis, Polymerase Chain Reaction (PCR) and its applications –TaqMan-PCR/RT-PCR
assays, RT-PCR to study cytokine/chemokine gene expression following viral infection, Microarray
based gene analysis, DNA and RNA isolations, DNA sequencing, Northern, Southern blotting.
Microbiology/Biotechnology: Isolation and identification of bacteria, maintaining bacterial and viral
cultures.
Cell Biology: Tissue culture, primary cell culture isolation and purification of feline peripheral blood
mononuclear cells PBMCs and lymph node cell subsets using flow cytometry, gene expression studies in
mammalian cells, and retroviral infection studies using acute infection, cell depletion, and co-culture
assays.
Immunology/Biochemistry: ELISA, Fluorescence Activated Cell Sorting (FACS) cytometric analysis
and cell fraction sorting, immunoblot cytokine/chemokine expression studies, magnetic bead cell sorting
and depletion, signal activation/blocking studies, polyacrylamide gel electrophoresis and Western
blotting.

Languages Jose Alvelo speaks:

English
Spanish
French
Italian
Portuguese