Problem Solver

Jose Alvelo

Areas Jose Alvelo is Knowledgeable in:

Over 6 years of all-source military intelligence experience, to include collection and analysis of
intelligence related to chemical/biological weapons, terrorism, and weapons of mass destruction in
support of Special Forces and Rapid-Deployment missions. More than 9 years of research experience in
various biological fields including; molecular and cell biology, microbiology, immunology, biochemistry,
and biotechnology in academia and military research programs. Over 3 years of teaching experience in
anatomy & physiology, Spanish, and English as a second language (ESL). Seven years of Science &
Technology Program Management for chemical and biological warfare defense for the Department of
Biodefense: Evaluation of cutting-edge biosensors technologies and development of molecular assays for
rapid identification of biological warfare agents from environmental samples. Provide program
management support to the Joint Science & Technology Office for Chemical and Biological Defense and
SME support to other DTRA organizations.
Nanoscience-Nanotechnology: Provide program management support to the Joint Science &
Technology Office for Chemical and Biological Defense Nanotechnology Initiative. Responsible for
selecting research projects for funding in support of CB defense applications.
Molecular Biology: Bacterial plasmid isolation, gene cloning and recombinant protein expression, gene
sequence analysis, Polymerase Chain Reaction (PCR) and its applications –TaqMan-PCR/RT-PCR
assays, RT-PCR to study cytokine/chemokine gene expression following viral infection, Microarray
based gene analysis, DNA and RNA isolations, DNA sequencing, Northern, Southern blotting.
Microbiology/Biotechnology: Isolation and identification of bacteria, maintaining bacterial and viral
Cell Biology: Tissue culture, primary cell culture isolation and purification of feline peripheral blood
mononuclear cells PBMCs and lymph node cell subsets using flow cytometry, gene expression studies in
mammalian cells, and retroviral infection studies using acute infection, cell depletion, and co-culture
Immunology/Biochemistry: ELISA, Fluorescence Activated Cell Sorting (FACS) cytometric analysis
and cell fraction sorting, immunoblot cytokine/chemokine expression studies, magnetic bead cell sorting
and depletion, signal activation/blocking studies, polyacrylamide gel electrophoresis and Western

Techniques Jose Alvelo Uses:

I am a business and technology strategy consultant and completed proposals to DHS, USDA, and NIH for a client in the nanotechnology arena. I am in the preparing additional proposals for DARPA, NIH, NSF, and US Army MRMC. I have also established a partnership between the same customer and a researcher from the Ecole Polytechnique Federale de Lausanne (Switzerland) two years ago. My goal is to assist in commercialization of biomedical technologies and engagement of researchers from the US and abroad. See attached resume for your review. My experience is with DOD, DOE National Labs, DHS, NIH, and NSF. I have done work with DOD/State Department on medical solutions for deployed US forces and humanitarian relief operations. In addition, I have extensive engagement and networking with NATO S&T community members and travel frequently to meet with researchers in Eastern Europe.

Jose Alvelo's Problem Solving Skills:

  1. Molecular Biology Techniques

Jose Alvelo's Problem Solving Experience:

  1. Responsibilities: Provide scientific advisement and/or program management services in support of the Norwich University Applied Research Institutes (NUARI), Institute for Advanced Sciences Convergence (IASC). Provide novel technology analysis and assessment support across IASC Programs. Provide analytical, programmatic support, managerial talent to further IASC’s goals to integrate its programs, specifically by providing technology management support to provide its’ customer base with S&T awareness on revolutionary and disruptive technologies and their potential national security implications; and support intra- and inter-agency coordination (e.g., DHS, DOE, TSWG, NASA, NSF, DARPA) as well as international S&T organizations.