Innovative People

Manesh Thomas

Technical Skills in Details- Microbiology, Molecular biology and Bioinformatics

Microbial aseptic techniques, culturing, isolation and purification, enumeration, preserving (glycerol, spore, stabs, lyophilized), Microbial Media preparations, Microscopy (simple, fluorescent, Phase contrast and Scanning Electron microscopy), All staining methods (cells, spore, capsule, flagella, Intracellular Granules), Morphological characterization and cell size measurement, Growth curve and determination of optimizing physiological conditions, Physiological Characterization (biochemical tests), Microbial interaction studies, Antibiotic Sensitivity test, Identification of unknown microbe, (Based on biochemical, APN, BIOLOG, Cultural, Physiological, 16s rDNA, ITS GYR gene, Rop gene sequence and FAME analysis by GC-MS.(MIDI), Microbial Metagenome analysis, Microbial limits tests, Sterility testing, Microbial contaminant identification, Preservatives testing and microbial challenges, Mycoplasma testing, Microbiological assessment of antibiotics, Bacterial endotoxin testing, Sampling and analysis of water for pharmaceutical microbiology, Chemical disinfectant testing and cleaning studies, Environmental Monitoring. Microbiological attribute studies (barrier testing: packaging, condoms, medical gloves; hygienic and antibacterial test: contact lenses, personal care products, etc.), Particulate testing.

Biotechnological and Molecular Biology
Isolation and estimation of DNA, RNA, protein, plasmid DNA, Isolation and characterization of Lipids Chromatography Separations (TFF, HPLC, GC, IC TLC and HPTLC), Isolation and purification of Metagenome from soil, water (also familiar with MO Bio KIT), PCR amplification of target genes, PCR condition optimization, Real Time PCR analysis (Quantitative and Qualitative), PCR product purification (Both column and GEL (Kits-Qiagen, Promega), Ligation, Cloning and transformation studies.(TA Cloning, pGMT, Topo TA), Clone Screening (Antibiotic, Blue-white selection)(JM109, DH5alpha), Preparation of Plasmid/PCR product for partil or Primer-walking sequencing, Electrophoresis-Agarose, IEF, PAGE, DGGE, 2-D, Genomic finger printing analysis through RFLP(Single, Double and Triple), AFLP, RAPD, Separation of mRNA from Total RNA, PolyA tailing, Reverse Transcription, cDNA and 2nd strand Synthesis,(Using Random Hexamers or RNase H), cDNA Library construction and storage, DDRT PCR for Gene expression.

Bioinformatics and software
1D, 2D Gel analysis software, gel scoring and similarity analysis by gel integration by software, Dendrogram, Phylogenetic Tree reconstruction, similarity index calculation, Sequence analysis, Editing of sequence, checking chimeras, checking sequence abnormalities, sequence alignment by different software., Familiar with Biological Data Bases, Familiar with Sequence analysis tools (Bank It, Sequin, FASTA, BLAST), MS Library search, 2D Protein gel analysis using PD-Quest, Primer Designing and software associated with below mentioned instruments were ever applicable.

Languages Manesh Thomas speaks:

English
Hindi
Malayalam