Problem Solver
Manesh Thomas
Areas Manesh Thomas is Knowledgeable in:
Technical Skills in Details- Microbiology, Molecular biology and Bioinformatics
Microbial aseptic techniques, culturing, isolation and purification, enumeration, preserving (glycerol, spore, stabs, lyophilized), Microbial Media preparations, Microscopy (simple, fluorescent, Phase contrast and Scanning Electron microscopy), All staining methods (cells, spore, capsule, flagella, Intracellular Granules), Morphological characterization and cell size measurement, Growth curve and determination of optimizing physiological conditions, Physiological Characterization (biochemical tests), Microbial interaction studies, Antibiotic Sensitivity test, Identification of unknown microbe, (Based on biochemical, APN, BIOLOG, Cultural, Physiological, 16s rDNA, ITS GYR gene, Rop gene sequence and FAME analysis by GC-MS.(MIDI), Microbial Metagenome analysis, Microbial limits tests, Sterility testing, Microbial contaminant identification, Preservatives testing and microbial challenges, Mycoplasma testing, Microbiological assessment of antibiotics, Bacterial endotoxin testing, Sampling and analysis of water for pharmaceutical microbiology, Chemical disinfectant testing and cleaning studies, Environmental Monitoring. Microbiological attribute studies (barrier testing: packaging, condoms, medical gloves; hygienic and antibacterial test: contact lenses, personal care products, etc.), Particulate testing.
Biotechnological and Molecular Biology
Isolation and estimation of DNA, RNA, protein, plasmid DNA, Isolation and characterization of Lipids Chromatography Separations (TFF, HPLC, GC, IC TLC and HPTLC), Isolation and purification of Metagenome from soil, water (also familiar with MO Bio KIT), PCR amplification of target genes, PCR condition optimization, Real Time PCR analysis (Quantitative and Qualitative), PCR product purification (Both column and GEL (Kits-Qiagen, Promega), Ligation, Cloning and transformation studies.(TA Cloning, pGMT, Topo TA), Clone Screening (Antibiotic, Blue-white selection)(JM109, DH5alpha), Preparation of Plasmid/PCR product for partil or Primer-walking sequencing, Electrophoresis-Agarose, IEF, PAGE, DGGE, 2-D, Genomic finger printing analysis through RFLP(Single, Double and Triple), AFLP, RAPD, Separation of mRNA from Total RNA, PolyA tailing, Reverse Transcription, cDNA and 2nd strand Synthesis,(Using Random Hexamers or RNase H), cDNA Library construction and storage, DDRT PCR for Gene expression.
Bioinformatics and software
1D, 2D Gel analysis software, gel scoring and similarity analysis by gel integration by software, Dendrogram, Phylogenetic Tree reconstruction, similarity index calculation, Sequence analysis, Editing of sequence, checking chimeras, checking sequence abnormalities, sequence alignment by different software., Familiar with Biological Data Bases, Familiar with Sequence analysis tools (Bank It, Sequin, FASTA, BLAST), MS Library search, 2D Protein gel analysis using PD-Quest, Primer Designing and software associated with below mentioned instruments were ever applicable.
Microbial aseptic techniques, culturing, isolation and purification, enumeration, preserving (glycerol, spore, stabs, lyophilized), Microbial Media preparations, Microscopy (simple, fluorescent, Phase contrast and Scanning Electron microscopy), All staining methods (cells, spore, capsule, flagella, Intracellular Granules), Morphological characterization and cell size measurement, Growth curve and determination of optimizing physiological conditions, Physiological Characterization (biochemical tests), Microbial interaction studies, Antibiotic Sensitivity test, Identification of unknown microbe, (Based on biochemical, APN, BIOLOG, Cultural, Physiological, 16s rDNA, ITS GYR gene, Rop gene sequence and FAME analysis by GC-MS.(MIDI), Microbial Metagenome analysis, Microbial limits tests, Sterility testing, Microbial contaminant identification, Preservatives testing and microbial challenges, Mycoplasma testing, Microbiological assessment of antibiotics, Bacterial endotoxin testing, Sampling and analysis of water for pharmaceutical microbiology, Chemical disinfectant testing and cleaning studies, Environmental Monitoring. Microbiological attribute studies (barrier testing: packaging, condoms, medical gloves; hygienic and antibacterial test: contact lenses, personal care products, etc.), Particulate testing.
Biotechnological and Molecular Biology
Isolation and estimation of DNA, RNA, protein, plasmid DNA, Isolation and characterization of Lipids Chromatography Separations (TFF, HPLC, GC, IC TLC and HPTLC), Isolation and purification of Metagenome from soil, water (also familiar with MO Bio KIT), PCR amplification of target genes, PCR condition optimization, Real Time PCR analysis (Quantitative and Qualitative), PCR product purification (Both column and GEL (Kits-Qiagen, Promega), Ligation, Cloning and transformation studies.(TA Cloning, pGMT, Topo TA), Clone Screening (Antibiotic, Blue-white selection)(JM109, DH5alpha), Preparation of Plasmid/PCR product for partil or Primer-walking sequencing, Electrophoresis-Agarose, IEF, PAGE, DGGE, 2-D, Genomic finger printing analysis through RFLP(Single, Double and Triple), AFLP, RAPD, Separation of mRNA from Total RNA, PolyA tailing, Reverse Transcription, cDNA and 2nd strand Synthesis,(Using Random Hexamers or RNase H), cDNA Library construction and storage, DDRT PCR for Gene expression.
Bioinformatics and software
1D, 2D Gel analysis software, gel scoring and similarity analysis by gel integration by software, Dendrogram, Phylogenetic Tree reconstruction, similarity index calculation, Sequence analysis, Editing of sequence, checking chimeras, checking sequence abnormalities, sequence alignment by different software., Familiar with Biological Data Bases, Familiar with Sequence analysis tools (Bank It, Sequin, FASTA, BLAST), MS Library search, 2D Protein gel analysis using PD-Quest, Primer Designing and software associated with below mentioned instruments were ever applicable.
Techniques Manesh Thomas Uses:
Research Experience
• Microbiology, Molecular biology, Cell biology and Microbial metabolism.
• Enzyme production, biotransformation, fermentation optimization and downstream.
• Microbial interactions, geochemistry, ecology and adaptation mechanisms.
• Biodiversity of microbial community (Archaea, Bacteria Actinomycets and Fungi).
• Screening isolates for traits in biotechnology, agriculture, antibiotics etc,
• Identification of 1400 bacteria, 72 archaea, 60 fungi based on molecular, biochemical and physiological characters.
• Molecular marker based characterization of isolates based on specific functional genes, using PCR, RT PCR, Sequencing based techniques.
• Molecular characterisation of biomolecules based on HPLC, IC, GC-MS, EDEX, Microscopy, HPTLC, TLC and Electrophoresis techniques(more than 1000 runs).
• Microbial community analysis through DGGE, Metagenomics, library construction, cloning and sequencing, role of microbial community through metabolic profiling.
• Phylogenetic study of novel archaeal and bacterial isolates with high end techniques.
• Study of mechanisms of stress adaptation by DDRT-PCR, Protein Profiling, cell physic-chemical characterization and RNA technologies.
• Bioinformatic processing, 215 Gene bank submissions, whole genome sequence submission of novel haloarchaea under processing.
• Microbiology, Molecular biology, Cell biology and Microbial metabolism.
• Enzyme production, biotransformation, fermentation optimization and downstream.
• Microbial interactions, geochemistry, ecology and adaptation mechanisms.
• Biodiversity of microbial community (Archaea, Bacteria Actinomycets and Fungi).
• Screening isolates for traits in biotechnology, agriculture, antibiotics etc,
• Identification of 1400 bacteria, 72 archaea, 60 fungi based on molecular, biochemical and physiological characters.
• Molecular marker based characterization of isolates based on specific functional genes, using PCR, RT PCR, Sequencing based techniques.
• Molecular characterisation of biomolecules based on HPLC, IC, GC-MS, EDEX, Microscopy, HPTLC, TLC and Electrophoresis techniques(more than 1000 runs).
• Microbial community analysis through DGGE, Metagenomics, library construction, cloning and sequencing, role of microbial community through metabolic profiling.
• Phylogenetic study of novel archaeal and bacterial isolates with high end techniques.
• Study of mechanisms of stress adaptation by DDRT-PCR, Protein Profiling, cell physic-chemical characterization and RNA technologies.
• Bioinformatic processing, 215 Gene bank submissions, whole genome sequence submission of novel haloarchaea under processing.
Manesh Thomas's Problem Solving Skills:
- Full time Researcher in life science
Manesh Thomas's Problem Solving Experience:
- Analysis on environmental samples, solving any technical problems statistical evaluation of data, revisions to methods, reporting, providing guidance and trainings with broad practical aspects and highly accurate techniques.