CpG island sequencing
The present invention is directed to a method for analyzing the methylation status of a genomic DNA sample, comprising the steps of (i) fragmenting said sample and enriching said sample for sequences comprising CpG islands, (ii) generating a single stranded DNA library, (iii) subjecting said sample to Bisulfite treatment, (iv) clonally amplifying individual members of said single stranded DNA library by means of emulsion PCR, and (v) sequencing said amplified clonally amplified single stranded DNA library.
FREY BRUNO [DE]; BETZL GISELA [DE]; FRITZILIAS EPAMEINONDAS [DE]; RAHMANN SVEN [DE]; HORSTHEMKE BERNHARD [DE]; ZESCHNIGK MICHA
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