Genetics Patents for Sale or License

Program for Determining the Level of Genetic Predisposition to High Achievements in Sports

The Success Scale computer programme has been developed to identify people of any age with a genetic predisposition to sporting achievement. The programme can help in the professional selection of children for fields of athletics in accordance with their genetic predispositions for success. The test results evaluate athletes' likelihood of success as one of the following three levels: High (a gold medal at the World Championships, Olympic Games or national championships), Middling (a silver or
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Method for Genetic Manipulation of Extremophiles

Provided herein are methods for introducing a polynucleotide into an extremophile, such as Pyrococcus fitriosis, by conjugation with a member of the family Enterobacteriaceae. Also provided are genetically engineered extremophiles, such as Pyrococcus furiosiis, that include an exogenous polynucleotide integrated into genomic DNA.
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Genetics Variants Associated with HIV Disease Restriction

The present invention relates, in general, to human immunodeficiency virus (HIV) and, in particular, to genetic variants associated with restriction of HIV disease progression.
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Signatures of Radiation Response

The present invention relates, in general, to gene expression profiles, and in particular, to a peripheral blood gene expression profile of an environmental exposure, ionizing radiation. The invention further relates to methods of screening patients for radiation exposure based on gene expression profiling and to kits suitable for use in such methods.
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Cross-species Chimeric RNA Molecules

Methods to reduce the expression of at least one gene of an intracellular pathogen in a host cell are provided, wherein the method comprises introducing into a host cell a heterologous polynucleotide comprising or encoding a miRNA that fuses with at least one mRNA of the pathogen and reduces the translation of the mRNA. Pharmaceutical compositions comprising such polynucleotides, wherein the reduction in pathogenic gene expression leads to an inhibition of growth or differentiation of the pathog
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Methods and Compositions for Treating Disorders Caused By a Deficiency in a Gene Product of a CLN Gene

The present invention provides methods and compositions for treating a disorder associated with a deficiency in a gene product of a CLN gene in a subject (e.g., neuronal ceroid lipofuscinosis (NCL)), comprising administering to the subject an effective amount of a sphingolipid (e.g., galactosylceramide, ceramide, lysophosphatidic acid, sulfatide and any combination thereof), thereby treating the disorder in the subject.
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Protein with Promoting Effects for Axonal Growth of Neurons of Central Nervous System

This invention comprises a polypeptide, a recombinant vector, a recombinant organism as well as RNA- and DNA- sequences. Furthermore, the use of polypeptides and recombinant vectors is described. Additionally the invention comprises methods for medical treatment.
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Monomeric Variants of He Tetrameric EqFP611

Monomeric eqFP611 variants exhibiting red fluorescent properties and DNA encoding monomeric eqFP611 variants are disclosed. Methods of use thereof are also provided.
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Living person 's biometric characteristic e.g. skin lines, detecting method, involves recording part of fingerprint pattern of finger, and detecting cyclic change of size of part of fingerprint patter

The method involves recording a part of a fingerprint pattern of a finger, and detecting a cyclic change in size of the part of the fingerprint pattern. A determination is made to find whether the finger is a part of a living person by evaluating the cyclic change of the size of the part of the fingerprint pattern. The cyclic change of the size of the part of the fingerprint pattern is detected by evaluating the images of the part of the pattern, where the images are recorded at different time p
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Methods for Modulating Immune Responses to AAV Gene Therapy Vectors

The present disclosure provides methods of inhibiting an immune response to a viral vector used in gene therapy, such as adeno-associated virus (AAV), which involves co-administration of viral vector and an interfering molecule. The interfering molecule functions by either disrupting the TLR9-MyD88-type I IFN signaling pathway and/or neutralizing Type I IFNs, thereby inhibiting the immune response directed against the viral vector. The methods additionally encompass the step of re-administering
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Immunomodulating gene therapy

The present invention relates, in general, to Pompe disease and, in particular, to a methods of treating Pompe disease and to compounds/constructs suitable for use in such methods.
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Lsamp gene associated with cardiovascular disease

The LSAMP gene can be used for cardiovascular disease risk assessment, in particular Left Main Disease. The genetic risk attributable to LSAMP adds to known cardiovascular disease risk factors. Assessment of risk attributable to LSAMP permits early initiation of preventive and therapeutic strategies. Given the pronounced clinical risk associated with Left Main Disease, such risk assessment should significantly reduce morbidity and mortality.
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Methods and Compositions for Correlating Genetic Markers with Cardiovascular Disease

The present invention provides methods of identifying a subject having an increased or decreased risk of developing cardiovascular disease, comprising: a) correlating the presence of one or more genetic markers in chromosome 3q13.31 with an increased or decreased risk of developing cardiovascular disease; and b) detecting the one or more genetic markers of step (a) in the subject, thereby identifying the subject as having an increased or decreased risk of developing cardiovascular disease. Also
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Alignancy-risk Signature from Histologically Normal Breast Tissue

The invention provides for malignancy-risk gene signatures that predict the risk of developing breast cancer, the recurrence of breast cancer, and/or the metastasis of breast cancer. These signatures have numerous clinical applications including assessing risk of breast cancer development following routine breast biopsy, assessing the need for adjuvant radiotherapy after lumpectomy, and determining the need for completion mastectomy following lumpectomy for the breast cancer patient and other tr
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Detection of Polyketide Synthetase Gene Expression in Karenia Brevis

The present invention concerns an assay for the detection of polyketide synthetase (PKS) mRNA from the red tide dinoflagellate Karenia brevis. Another aspect of the invention concerns NASBA primers and a molecular beacon that enable detection of mRNA of one of the PKS genes. In another aspect, the present invention includes an internal control RNA (IC-RNA) that enables quantitative detection of the target as well as correction for inhibition of amplification. The assay of the invention provides
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Gene Signature for the Prediction of Radiation Therapy Response

Described are mathematical models and method, e.g., computer-implemented methods, for predicting tumor sensitivity to radiation therapy, which can be used, e.g., for selecting a treatment for a subject who has a tumor.
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LPS-Responsive CHS1/Beige-Like Anchor Gene and Therapeutic Applications Thereof

The present invention relates to a novel LPS-responsive and Beige-like Anchor gene (lrba), variants of the lrba gene, fragments of the lrba gene, and polypeptides encoded thereby. The subject invention also pertains to lrba interfering RNA, and uses thereof. In another aspect, the present invention also includes methods of inhibiting tumor growth in a patient by suppressing lrba function.
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Sphingosine 1-Phosphate Receptor Gene, SPPR

A novel sphingosine 1-phosphate receptor gene, herein termed sppr, and its splice variants. Sppr is up-regulated in LGL and is useful, for example, in the diagnosis and treatment of certain lymphoproliferative, neurodegenerative and autoimmune diseases.
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Seven Gene Breast Cancer Predictor

The invention provides a molecular marker set that can be used for prognosis of breast cancer in a patient using histologically normal tissue. The invention also provides methods for evaluating prognosis of breast cancer in a patient based on a molecular molecular signature.
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Method of Predicting Chemotherapeutic Responsiveness of Cancer

Disclosed is a method of predicting clinical tumor outcome by providing gene expression from a tumor sample. The method utilizes a novel genetic screen to identify genes that contribute to chemotherapeutic responsiveness, using formalin fixed paraffin embedded clinical samples of epithelial cancer, specifically serous ovarian cancer. The method is useful in predicting tumor responsiveness to chemotherapeutics, including alkylating agents, cisplatin, antimetabolites, plant alkaloids, and antitumo
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Predictive Value of Nuclear Excision Repair Genes for Cancer Survival

Patients who have their cancer surgically removed are at risk for recurrence, even though currently free of disease. Lung cancer patients with higher activity of a nuclear excision repair gene called ERCC1 were at a lower risk for recurrence than patients with lower activity of ERCC1. Hence, it is possible to predict which patients are at a higher risk for recurrence after resection. This information can be used to design treatment strategies for patients determined to be at a higher risk for re
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Chemokine Gene-modified Cells for Cancer Immunotherapy

Described herein are methods of cancer immunotherapy, particularly compositions comprising genetically-modified cells that express macrophage colony stimulating factor (GM-CSF), CD40 ligand (CD40L), and chemokine C-C motif ligand 21 (CCL21), wherein the population of cells comprises bystander cells and target cancer cells, and methods of making these compositions and treating cancer using these compositions.
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Genetically engineered co-expression DNA vaccines, construction methods and uses thereof

The present invention relates to co-expression DNA vaccines and methods for vaccinating animals against viral, bacterial and parasitic pathogens. In particular, the present invention relates to DNA vaccines that co-express antigens in combination with biologically-active components, such as adjuvants, immunoregulatory agents, antisense RNAs, and/or catalytic RNAs.
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Genetic Variants in a Hypertension Susceptibility Gene STK39 and Uses Thereof

The present invention is drawn to diagnosis and treatment of essential hypertension. In this regard, the present invention discloses genetic variants in a hypertension susceptibility gene Stk39 and its use in the diagnosis and treatment of essential hypertension.
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Methods and Compositions for Genetically Engineering Clostridia Species

The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species.
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Expressed Pseudogene Regulates Gene Expression

Selective expression of a pseudogene of myosin light chain kinase is found in cancer cells and tissues but not in normal cells and tissues. The pseudogene is expressed, and when expressed it inhibits expression of the ancestral myosin light chain kinase. This widespread expression among cancer cell types and the selective expression in cancer cells versus normal cells opens the door to many diagnostic and therapeutic applications.
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Methods for Cloning Ferrets and Transgenic Ferret Models for Diseases

The invention provides a transgenic Mustelidae in which a gene associated with a human disease or condition comprises a targeted genetic modification, and uses thereof. Also provided is a method to cryopreserve Mustelidae embryos or cells, and to enhance the number of live offspring from cryopreserved Mustelidae embryos.
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Gene Chip Technology for Determining Memory Genes

The present invention relates to methods of identifying genes involved in memory formation. This is accomplished by performing a gene chip identification of those genes expressed during transcription-dependent memory formation but not during trancription-independent memory formation. A statistical analysis of the gene chip identification output yields a set of genes that are involved in transcription-dependent memory formation.
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Prediction and Diagnosis of Canine Degenerative Myelopathy.

The present invention provides for methods of identifying a dog carrying a major genetic risk factor in the SODl gene for degenerative myelopathy, a potential model for human amyeotrophic lateral sclerosis. Also provided a methods of early diagnosis, treatment and breeding based on the presence or absence of the marker.
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Isolation of five novel genes coding for new Fc receptors-type melanoma involved in the pathogenesis of lymphoma/melanoma

This invention provides an isolated nucleic acid molecule which encodes immunoglobulin receptor, Immunoglobulin superfamily Receptor Translocation Associated, IRTA, protein. Provided too, are the IRTA proteins encoded by the isolated nucleic acid molecules, IRTA1, IRTA2, IRTA3, IRTA4 or IRTA5 proteins, having the amino acid sequences set forth in any of Figures 18A , 18B-1-18B-3 , 18C-1-18C-2 , 18D-1-18D-2 or 18E-1-18E-2 . Oligonucleotides of the isolated nucleic acid molecules are provided. Ant
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Psoriatic Phenotype Mice, Cell Lines, Treatments, and Methods

A genetically-modified, non-human animal, ES cells and methods for diagnosing and identifying agents useful in the treatment of psoriasis, and psoriatic conditions or disorders is provided.
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Gene Differentially Expressed in Breast and Bladder Cancer and Encoded Polypeptides

The present invention relates to a novel human gene that is differentially expressed in human carcinoma. More specifically, the present invention relates to a polynucleotide encoding a novel human polypeptide named C35 that is overexpressed in human breast and bladder carcinoma. This invention also relates to C35 polypeptide, in particular C35 peptide epitopes and C35 peptide epitope analogs, as well as vectors, host cells, antibodies directed to C35 polypeptides, and the recombinant methods for
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Risk Stratification of Genetic Disease Using Scoring of Amino Acid Residue Conservation in Protein Families

Methods, databases and software for determining the risk of an adverse health event for patient by analysis of the protein sequence of the patient are described. The methods involve obtaining a protein sequence that is associated with a specific disorder from the patient. The protein sequence from the patient is compared to a database of sequences of the same protein and is analyzed to determine the conservation score of the amino acid residues in the protein. Those amino acid residues having hi
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Enhancing Gene Transfer

Described herein are methods of improving the efficiency of gene transfer for a wide range of applications. Specifically provided are methods of increasing expression of an exogenous gene in a cell by contacting the cell with a vector comprising the exogenous gene and contacting the cell with a proteasome inhibitor, a lysosomal protease inhibitor and/or a microtubule inhibitor. Also provided are methods of delivering an antigen delivery vector to a cell or a subject. Provided are antigen deliver
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Piggybac Transposon Variants and Methods of Use

The present invention provides hyperactive piggyBac transposons, in particular hyperactive piggyBac transposons from Trichoplusia ni (cabbage looper moth) that transpose at a higher frequency than wildtype. The invention also features integration defective piggyBac transposons. The piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to,
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Mammalian Piggybac Transposon and Methods of Use

The present invention provides mammalian piggyBac transposons and transposases. In particular embodiments, the present inventors have identified hyperactive mammalian piggyBac variants. The mammalian piggyBac transposons and transposases can be used in gene transfer systems for stably introducing nucleic acids into the DNA of a cell. The gene transfer system can be used in methods, for example, but not limited to, gene therapy, insertional mutagenesis, or gene discovery.
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Conversion of Chitin Into N-acetylglucosamine, Glucosamine and Bioethanol

Compositions and methods are provided for converting chitin into N-acetylglucosamine, glucosamine and ethanol. The chitin may be used directly from the environment, for example, as occurs in invertebrate cuticles, fungal cells and/or algae. Mutant bacteria were created by knocking out or inactivating one or more genes preferably resulting in the chitin catabolic sensor maintaining an activated state. Methods are further provided for converting the N-acetylglucosamine into ethanol by means of a g
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Ubiad1 Gene and Hyperlipidemia

The disclosure relates to genetic mutations in UBIAD1 gene that segregate with Schnyder's crystalline corneal dystrophy. The disclosure provides methods for detecting such mutations as a diagnostic for Schnyder's crystalline corneal dystrophy either before or after the onset of clinical symptoms. Also provided are screening methods for identifying medical conditions related to cholesterol metabolism, including atherosclerosis, risk of future loss of vision, and future need for corneal transplant
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Method and Means for Activating Memory B Lymphocytes

The invention relates to a method and means for activating memory B lymphocytes, in particular for use in immunological research and in medicine. For the inventive method, the cells are treated with an activating reagent containing pokeweed mitogen, CpG oligonucleotides, IL-2 and IL-10. It is surprising that IL-2, IL-10 and CpG oligonucleotides enhance the activating effect of pokeweed mitogen. The inventors have found that the addition of IL-6, LPS, CD40 ligand or anti-CD40 antibodies, does not
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Vector(s) Containing an Inducible Gene Encoding a Cdk4/cdk6 Inhibitor Useful for Treating Neurodegenerative Disorders Or Diseases Associated with an Unscheduled Activation of the Cell Cycle

Described are vectors containing (a) a gene encoding (i) a CDK4/CDK6 inhibitor, preferably p16INK4a, or (ii) an RNA interfering with CDK4 and/or CDK6 expression and/or activity, under the control of an inducible promoter and (b) a gene encoding a transactivator protein for said promoter useful for treating neurodegenerative disorders. These vectors can be transferred into cells where they will exert a protective function to (i) prevent cell death or to (ii) slow down progression of cell death. T
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Mycobacterium tuberculosis persistance genes

Compositions and methods for preventing Mycobacterium infections, particularly persistent, latent Myc?bactial infections, are provided. The compositions and methods utilize newly identified Mycobacterium genes (and/or corresponding gene products) that are necessar for survival of Mycobacteriae within a host.
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Human Transcriptomes

Global gene expression patterns have been characterized in normal and cancerous human cells using serial analysis of gene expression (SAGE). Cancer cell-specific, cell-type specific, and ubiquitously expressed genes have been identified. This information can be used to provide combinations of cell type- and cancer-specific gene probes, as well as methods of using these probes to identify particular cell types, screen for useful drugs, reduce cancer-specific gene expression, standardize gene expr
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Use of a High-resolution Melting Assay to Measure Genetic Diversity

A method is provided for determining the level of genetic diversity in a sample using a high-resolution melting assay.
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Genetic Alterations in Isocitrate Dehydrogenase and Other Genes in Malignant Glioma

We found mutations of the R132 residue of isocitrate dehydrogenase 1 (IDH1) in the majority of grade II and III astrocytomas and oligodendrogliomas as well as in glioblastomas that develop from these lower grade lesions. Those tumors without mutations in IDH1 often had mutations at the analogous R172 residue of the closely related IDH2 gene. These findings have important implications for the pathogenesis and diagnosis of malignant gliomas.
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Compositions and Methods for Biofuel Crops

Using the natural variation of sweet and grain sorghum to uncover genes that are conserved in rice, sorghum, and sugarcane, but differently expressed in sweet versus grain sorghum by using a microarray platform and the syntenous alignment of rice and sorghum genomic regions containing these genes. Indeed, enzymes involved in carbohydrate accumulation and those that reduce lignocellulose can be identified. Interestingly, C4 photosynthesis is enhanced as well. Furthermore, genetic analysis has sho
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Therapeutic Modulation of Autophagy

Methods for screening for modulators of autophagy are disclosed. Methods for identifying genes whose expression inhibits autophagy, as well as genes whose expression promotes autophagy, are disclosed. Also disclosed are methods for identifying compounds that stimulate autophagy, as well as compounds that inhibit autophagy. Cell lines that may be used in the methods of identification are also disclosed.
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Media Conditioning for Improving Gene Delivery Efficiency to Differentiating Embryonic Stem Cells

The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from ~1.4% to ~3.7% of the population.
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Systems and Methods for Identification of Ciliopathy Therapeutics

The invention provides systems and methods for identifying therapeutic targets for treating a disease including a ciliopathy. The invention further provides for drug discovery, and animal model systems related to drug discovery. The invention further relates to therapy of genetic disorders of the cellular cilia or basal bodies.
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Independently Inducible System of Gene Expression

The present invention is directed to the improved methods for the temporal induction of proteins using the condensed single protein production (cSPP) system.
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Genes As Diagnostic Tools for Autism

The homeobox transcription factor, ENGRAILED 2 EN2), was investigated for association with autism spectrum disorder (AS) by performing transmission/disequilibrium tests (TDT) for two SNPs (rs1861972 and rs1861973) and was identified as being a likely susceptibility locus for autism and related AS disorders. Initially, TDT was performed using 137 triads of autistic individuals and their parents and significant overtransmission of the A allele of rs1861972 and the C allele or rs1861973 was observe
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MicroRNAs for Modulating Herpes Virus Gene Expression

An algorithm for identification of microRNA (miRNA) targets within viral and cellular RNA is disclosed. Also disclosed are essential herpes virus genes whose transcripts contain one or more targets of miRNAs encoded by herpes viruses or by host cells as predicted by the algorithm, and the use of such targets, miRNAs and their derivatives for modulating viral replication and latency.
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Compositions and Methods for Regulating Rna Translation Via Cd154 Ca-dinucleotide Repeat

Compositions and methods for regulating CD154 gene expression are provided that rely on the interaction of hnRNP L with the CA-dinucleotide rich sequence of the 3 ' - untranslated region of CD154.
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Modified Cipa Gene from Clostridium Thermocellum for Enhanced Genetic Stability

Bacteria consume a variety of biomass-derived substrates and produce ethanol. The scaffoldin gene cipA from Clostridium thermocellum is modified to generate a mutated gene with enhanced genetic stability. This mutated cipA gene can be introduced into a heterologous host, such as Thermoanaerobacterium saccharolyticum. Other cellulosome components may be introduced into the host to build a full-sized cellulosome in T. saccharolyticum. Manipulation of the scaffoldin genes provides a new approach fo
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Genes Encoding the Biosynthetic Pathway for Etnangien Production

The present invention provides the genes encoding the biosynthetic pathway enzymes and the biosynthetic pathway enzymes for producing in a host micro-organism the compound etnangien. The invention provides the coding sequences and the respective translation products obtainable therefrom, which constitute the coding sequences and the catalytically active translation products obtainable therefrom, necessary for the biosynthetic pathway for the production of etnangien in micro-organisms.
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CpG island sequencing

The present invention is directed to a method for analyzing the methylation status of a genomic DNA sample, comprising the steps of (i) fragmenting said sample and enriching said sample for sequences comprising CpG islands, (ii) generating a single stranded DNA library, (iii) subjecting said sample to Bisulfite treatment, (iv) clonally amplifying individual members of said single stranded DNA library by means of emulsion PCR, and (v) sequencing said amplified clonally amplified single stranded D
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Perforated Membranes

The present invention relates to a membrane with at least one molecular mono layer built up of low molecular aromatics and cross-linked in the lateral direction, wherein the membrane comprises a thickness in the range from 1 to 200 nm and a perforation in form of openings with a diameter in the range from 0.1 nm to 1 [mu]m. The invention further relates to a method for the production thereof and use thereof.
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Identification of Regulatory T Cells Via the Global Gene Regulator Satb1

The present invention provides a method for the identification of regulatory T cells based on the diminished abundance or even absence of the global gene regulator SATB1 in such regulatory T cells. In particular, the invention relates to a method utilizing ligands that specifically bind to SATB1 for identifying regulatory T cells which are cells showing a reduced binding to said ligand. Such method is suitable for quality determination of a regulatory T cell population. The invention further pro
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Nucleic Acid Expression Construct and Its Use As a Cell Proliferation Marker

In a first aspect, the present invention provides a nucleic acid expression construct encoding a fusion protein comprising a reporter protein and a protein with a wild-type destruction signal, wherein the sequence encoding the fusion protein is operably linked to a non-endogenous promoter, and wherein the fusion protein localizes during cell cycle progression to subcellular structures selected from the group consisting of the cell cortex, the contractile ring, and the midbody. In a further aspec
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Methods, Apparatuses, and Kits for Introducing Genetic Material Into Living Cells

Methods, kits and devices are provided for introducing genetic material into cells, including post-mitotic types of cells such as neurons and sperm cells. The methods and devices utilize high fluid velocities, and cell survival following treatment by the methods including the loading process is enhanced compared to that of other transfection methods. The methods result in reliable highly efficient and rapid expression of recombinant proteins in the recipient cells.
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Genetic Association of Polymorphisms in Perilipin (plin) Gene with Resistance to Weight Loss

Diagnostics and therapeutics for resistance to weight-loss, which are based upon the identification of a subject's PLIN polymorphisms, haplotype and genotype pattern, are described in this invention.
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Genetic Markers for Obesity

The present invention is directed to new genetic variants or polymorphisms at the perilipin locus (PLIN) including PLIN1: 6209T (allele 1)>C (allele 2); PLIN3 10171 (allele 1) A >T (allele 2); PLIN4: 11482G (allele 1)>A (allele 2); PLIN5: 13041A (allele 1)>G (allele 2) and PLIN6: 14995A (allele 1)>T (allele 2), and their use in diagnostic and prognostic applications for obesity and obesity-related diseases, such as metabolic syndrome and cardiovascular disease.
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Neonatal Salivary Genomics

The present invention provides systems for assessing neonatal development and/or conditions by analyzing neonatal saliva RNA. Methods of identifying genes involved in neonatal development and/or conditions affecting neonates, are provided. Methods of determining a diagnosis of a neonate comprising detection of one or more differentially expressed genes are also provided.
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Modular Nucleic Acid-based Circuits for Counters, Binary Operations, Memory, and Logic

We have created novel engineered genetic counter designs and methods of use thereof that utilize DNA recombinases to provide modular systems, termed single invertase memory modules (SIMMs), for encoding memory in cells and cellular systems. Our designs are easily extended to compute to high numbers, by utilizing the >100 known recombinases to create subsequent modules. Flexibility in our engineered genetic counter designs is provided by daisy-chaining individual modular components, i.e., SIMMs t
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Compositions and Methods for Silencing Ebola Virus Gene Expression

The present invention provides compositions comprising therapeutic nucleic acids (e.g., interfering RNA such as siRNA) that target Ebola virus (EBOV) gene expression and methods of using such compositions to silence EBOV gene expression. More particularly, the invention provides unmodified and chemically modified interfering RNA which silence EBOV gene expression and methods of use thereof, e.g., for preventing or treating EBOV infections caused by one or more EBOV species such as Zaire EBOV. Th
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In Vivo Gene Sensors

Described are methods and compositions for the detection of target genes. The inventors have developed a synthetic nucleic acid sensor-effector gene circuit. In cells without a target gene, the circuit suppresses e.g., effector production, but in the presence of the target gene the suppression is subject to competition, such that the synthetic sensor is de-repressed and permits expression of the effector gene. The methods and compositions described further permit the selective expression of an e
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Genetic Inhibition By Double-stranded Rna

PROBLEM TO BE SOLVED: To provide a process for introducing an RNA into a living cell to inhibit gene expression of a target gene in the cell. ; SOLUTION: The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the double stranded chain region of the RNA and of a portion of the target gene are identical. The inhibition is distinguished from prior art inhibition in gene expression by a
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Novel Aav 's and Uses Thereof

The invention in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the invention relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the invention relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.
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Single-nucleotide Polymorphism (snp) Targeting Therapies for the Treatment of Huntington's Disease

The present invention relates to the discovery of (SNPs) significantly associated with Huntington's disease (HD). The present invention utilizes RNA silencing technology (e.g. RNAi) against such SNPs optimally combined with select additional SNP targeting silencing agents, thereby resulting in an effective treatment of significantly- sized patient populations. Silencing agents having enhanced discriminatory properties are also featured.
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In Vivo Production of Small Interfering Rnas That Mediate Gene Silencing

The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both t
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Isolation of Novel AAV'S and Uses Thereof

The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA.
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Methods and Compositions for Locating Snp Heterozygosity for Allele Specific Diagnosis and Therapy

The present invention provides methods for the rapid and cost effective identification of the presence of a disease-associated mutation and a particular SNP in the same allele of a gene without the need to clone and sequence the entire gene. The compositions and methods of the invention are useful for identification of patient to subpopulations amenable to treatment as part of a therapeutic strategy for treating genetic disorders, for example, dominant, gain-of-function gene mutations, for examp
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Methods for Production of Pluripotent Cells

Methods for obtaining pluripotent (embryonic stem) cells from parthenogenetic embryos, especially primates, are provided. These cells are useful for producing differentiated cells, tissues and organs, especially human and non-human primate cells, tissues and organs.
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Methods and compositions for controlling efficacy of RNA silencing

Based at least in part on an understanding of the mechanisms by which small RNAs (e.g., naturally-occurring miRNAs) mediate RNA silencing in plants, rules have been established for determining, for example, the degree of complementarity required between an RNAi-mediating agent and its target, i.e., whether mismatches are tolerated, the number of mismatches tolerated, the effect of the position of the mismatches, etc. Such rules are useful, in particular, in the design of improved RNAi-mediating
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Methods and Compositions for Improving the Production of Products in Microorganisms

Methods and compositions are provided for improving the production of products, such as fuel products like ethanol, in microorganisms. In particular, methods and compositions are described for improving ethanol production utilizing genes identified in Clostridium phytofermentans.
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Mutations in Contaction Associated Protein 2 (cntnap2) Are Associated with Increased Risk for Ideopathic Autism

The present invention provides compositions and methods for the examination of cells, tissues, and fluids, collectively known as body samples, to identify human subjects at-risk of developing Autism Spectrum Disorder by detecting a chromosomal abnormality or variant in the CNTNAP2 gene, the AUTS2 gene, or both.
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Riboswitches, methods for their use, and compositions for use with riboswitches

It has been discovered that certain natural mRNAs serve as metabolite-sensitive genetic switches wherein the RNA directly binds a small organic molecule. This binding process changes the conformation of the mRNA, which causes a change in gene expression by a variety of different mechanisms. Modified versions of these natural "riboswitches" (created by using various nucleic acid engineering strategies) can be employed as designer genetic switches that are controlled by specific effector compounds
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Nucleotide and Protein Sequence of Vertebrate Serrate Gene, and Method Based Thereon

PROBLEM TO BE SOLVED: To provide a nucleotide sequence of vertebrate Serrate gene, to provide the amino acid sequence of the protein encoded thereby, and further, to provide a derivative thereof (e.g. fragment) and an analogue thereof. ; SOLUTION: A refined vertebrate Serrate protein is provided. In one embodiment, there are provided a vertebrate Serrate derivative and analogue, which can exhibit one or more well-known functional activities associated with the functionally active, namely, full-l
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Compostions and Methods for Targeted Gene Therapy

Compositions containing molecules that bind to duplex DNA in a sequence-specific manner to form a triple-stranded structure are disclosed. Triplex-forming molecules facilitate strand displacement and triplex formation, referred to as a "clamp," in which one molecule binds to the target strand by Hoogsteen binding and the other molecule binds to the target strand by Watson-Crick binding in a sequence specific manner. A Watson- Crick binding "tail" added to the end of the Watson-Crick binding port
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Polymeric Materials Loaded with Mutagenic and Recombinagenic Nucleic Acids

Polymeric rnicroparticles are used to deliver recombinagenic or mutagenic nucleic acid molecules such as donor nucleic acid alone, or in combination with triplex forming molecules, to induce a site-specific mutation in the target DNA. Target cells endocytose the particles, releasing the nucleic acid molecules inside of the cell, where they induce mutagenesis or recombination at a target site. The examples demonstrate that triplex forming oligonucleotides, preferably PNAs, preferably in combinati
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A Melanoma Prognostic Model Using Tissue Microarrays and Genetic Algorithms

Provided is a method for determining the risk that a patient diagnosed with melanoma will develop a recurrence of melanoma comprising: a) determining the level of expression for each marker of a panel of markers, wherein the panel comprises activating transcription factor 2, p21V1AF1, p16NK4A, beta-catenin, and fibronectin and the levels of expression are determined in compartments of interest in a tumor tissue sample from the patient; and b) determining whether an expression parameter for each
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Pseudocomplementary Oligonucleotides for Targeted Gene Therapy

Compositions and methods for targeted gene therapy are disclosed. Compositions containing double duplex-forming pseudocomplementary oligonucleotides are administered in combination with a donor oligonucleotide that is homologous to a target sequence on a double-stranded DNA molecule in need of repair or replacement. By activating cellular mechanisms involved in DNA synthesis, repair and recombination, the double duplex-forming pseudocomplementary oligonucleotides can introduce one or more mutati
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Regulation of oncogenes by microRNAs

Naturally occurring miRNAs that regulate human oncogenes and methods of use thereof are described. Suitable nucleic acids for use in the methods and compositions described herein include, but are not limited to, pri-miRNA, pre-miRNA, mature miRNA or fragments of variants thereof that retain the biological activity of the mature miRNA and DNA encoding a pri-miRNA, pre-miRNA, mature miRNA, fragments or variants thereof, or regulatory elements of the miRNA. The compositions containing nucleic acids
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Targeted Oligonucleotide Compositions for Modifying Gene Expression

The invention comprises compositions and methods for modifying gene expression. Modified oligos of the invention restore the lost function of let- 7 wild type miRNA molecules that are prevented from silencing target genes by mutations occurring within their binding sites. Administration of a particular modified oligo (SEQ ID NO: 22) leads to increased cell death in cancer cells carrying the LCS6 SNP.
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Riboswitches, methods for their use, and compositions for use with riboswitches

It has been discovered that certain natural mRNAs serve as metabolite-sensitive genetic switches wherein the RNA directly binds a small organic molecule. This binding process changes the conformation of the mRNA, which causes a change in gene expression by a variety of different mechanisms. Modified versions of these natural "riboswitches" (created by using various nucleic acid engineering strategies) can be employed as designer genetic switches that are controlled by specific effector compounds
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Method for Detecting and Identifying Species of the Family Sparidae

Method for detecting and identifying species of the family Sparidae. The present invention relates to a method for detecting and identifying the species Pagrus pagrus, Pagrus auriga and Diplodus sargus, by using PCR techniques for amplifying the sequence of the ITS-1 region of the 45S rDNA ribosomal gene. Sequences isolated from the ITS-1 region of said species, not previously described, are likewise covered, as are the oligonucleotides for amplifying said sequences. Lastly, the invention relate
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Gene E for Antitumour Treatment

Gene E for antitumour treatment. Use of gene E of bacteriophage X174, of the amino acid sequence thereof and of pharmaceutical compositions containing same, which, inserted in and expressed in cells derived from malignant tumours, and especially melanoma, is capable of inducing lesions therein that give rise to cell death thereof.
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Method for the Identification of Carcinogenic Compounds

The present invention relates to methods for the identification of genotoxic carcinogenic compounds. In particular, a method is disclosed for the identification of genotoxic carcinogenic compounds wherein a eukaryotic cell is exposed to a potentially genotoxic compound in a culture medium where after samples are taken from the cell and/or the culture medium at at least one predetermined time point which samples are then analysed for increased or decreased expression levels of at least three DNA
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Novel mRNA splice variant of the doublecortin-like kinase gene and its use in cancer diagnosis and therapy

Methods for treating neuroblastoma in a subject are provided. The methods include administering to the subject nucleic acid that is capable of causing a significant reduction of the amount of doublecortin like protein (DCL) in the subject. The nucleic acids include antisense oligonucleotides and small interfering RNA.
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A Method for Regulating Eukaryotic Gene Expression At the Level of Chromatin

The present invention relates to methods for inducing changes in epigenetic gene control in a chromatin domains in eukaryotic cells, and to nucleic acid constructs and host cells for use in such methods. The methods use nucleic acid constructs that are preferablyintegrated at a predetermined chromatin region of the host cell's genome, which nucleic acid construct comprise binding sites for a DNA-binding protein. Proteins that induces or are suspected to induce epigenetic gene control may then be
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Recombinant Pichia Pastoris Cell

The present invention relates to a recombinant Pichia pastoris cell comprising at least one mutation in the dihydroxyacetone synthase gene 2 (DAS2) resulting in a reduced dihydroxyacetone synthase activity compared to a wild-type Pichia pastoris cell, wherein the DAS2 gene comprises a nucleotide sequence having at least 80% identity with SEQ ID No. 1.
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Method and kit for detecting genetic predisposition for crooked tail syndrome (CTS) in bovine individuals

The present invention provides the use of the MRC2 gene locus coding for the Endo180 protein as marker for detecting whether or not a bovine individual is carrier of Crooked Tail Syndrome (CTS). The present invention further refers to an in-vitro method for detecting whether or not a bovine individual is carrier of Crooked Tail Syndrome, by analyzing the MRC2 gene locus coding for the Endo180 protein. The invention also provides an assay for detecting whether or not a bovine individual is carrie
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Hydrolysis of Mannose-1-phospho-6-mannose Linkage to Phospho-6-mannose

Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.
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Screening Method for Identifying Genes Involved in Plant Cell Cycle

The present invention relates to a novel method for screening proteins related to and/or involved in plant cell cycle. It further relates to proteins isolated with the method, and the use of those proteins, and/or the genes encoding those proteins for modulating plant yield and plant growth.
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Nucleic Acids of Pichia Pastoris and Use Thereof for Recombinant Production of Proteins

The present invention provides the genome sequence of Pichia pastoris and manually curated annotation of protein-coding genes. The invention provides novel nucleic acids, proteins, and related expression vectors useful for genetic engineering of methylo trophic yeast strains, as well as engineered methylotrophic yeast strains particularly Pichia pastoris, and use thereof for recombinant production of heterologous proteins including glycoproteins suitable for use in mammals including humans.
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Production of Multi-antennary N-glycan Structures in Plants

The invention provides methods for producing multi-antennary glycoproteins in plant and plant cells. In particular the invention provides plants comprising a chimeric gene comprising glucosaminyltransferase IV and plants comprising two chimeric genes comprising glucosaminyltransferase IV and V.
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Novel Crustacean Androgenic Gland Hormone

The present invention relates to a novel crustacean androgenic gland hormone. More specifically, the invention relates to an androgenic gland hormone derived from Penaeus monodon. The invention relates further to the use of the hormone to influence the sex ratio in prawn and shrimp cultures, and to set up monosex cultures. It is also related to the use of the hormone and its gene or fragments thereof in sex determination.
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Liver-specific Nucleic Acid Regulatory Elements and Methods and Use Thereof

The present invention relates to nucleic acid regulatory elements that are able to enhance liver-specific expression of genes, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy.
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Cardiac-specific Nucleic Acid Regulatory Elements and Methods and Use Thereof

The present invention relates to nucleic acid regulatory elements that are able to enhance cardiac- specific expression of genes, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy.
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Method to Control Spider Mites

The present invention relates to a method to control spider mites on plants. More specifically, the invention relates to plants, expressing RNAi of one or more essential genes of the spider mite, and the use of those plants to control the spider mite proliferation into pest proportions. In a preferred embodiment, the spider mite is Tetranychus urticae.
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Hydrolysis of Mannose-1-phospho-6-mannose Linkage to Phospho-6-mannose

Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.
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Protein glycosylation modification in Pichia pastori

The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for +--1,2-mannosidase I, glucosidase II, G1cNAc-tranferase I and mannosidase II or comprising OCH1 disrup
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Minichromosome Maintenance Complex Interacting Protein Involved in Cancer

The present invention relates to a protein that is interacting with the minichromosome maintenance complex in eukaryotes. More specifically, the invention relates to the use of a protein, interacting with the minichromosome complex, and the gene encoding this protein in diagnosis, prognosis and treatment of cancer.
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Tools and Methods for Genetic Tests Using Next Generation Sequencing

The present invention provides tools and methods for use in genetic tests involving high performant sequencing techniques. More particularly, the invention provides a robust multiplex PCR method wherein the respective primers for amplifying the different amplicons are physically isolated from one another. The invention further provides quality control methods allowing a stringent monitoring of genetic tests carried out according to the present invention.
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Haplotype of Katiii Gene

The present invention relates to a diagnostic marker comprising at least one single nucleotide polymorphism (SNP) in the human KATIII gene, as well as to a method for the detection of a predisposition for a psychiatric disorder or a psychiatric disease.
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Mucosal Gene Signatures

Infliximab (IFX) is effective treatment for Crohn's disease (CD) and ulcerative colitis (UC) not responding to standard therapy. 30-40% of patients do not improve and response is often incomplete. The aim of this study is to identify mucosal gene signatures predictive of response to EFX using high-density oligonucleotide arrays. Eight UC patients and 12 CD patients showed healing. In UC, only one probe set was differentially expressed in responders compared with non-responders, ie IL-13Ralpha2.
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Method for Detecting or Quantifying a Truncating Mutation

The present invention discloses a new method for detecting or quantifying a truncating mutation of a target gene in a subject, said method relying on the in vitro compartmentalization of single genetic constructs in aqueous droplets of a water-in-oil emulsion.
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Method and Device for Reading an Emulsion

The present invention relates to a method for reading an emulsion (3) comprising drops and a continuous phase surrounding the drops, wherein said method comprises: two-dimensional scanning of the emulsion (3), and construction of a two-dimensional image of the emulsion (3) on the basis of said scanning. Preferably, the drops do not move during the scanning, this being achieved, for example, by solidifying the continuous phase or by using a compact or semi-compact two-dimensional network of drops
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Non-auxotrophic Selection Marker

A non-auxotrophic selection marker located in a gene cluster encoding a metabolite for an organism, such as a fungus, lacking said marker is provided. The selection marker gene can be used to detect and confirm the introduction of foreign genes without the use of exogenous drug-resistant genes to facilitate transformant selection. The selection marker gene can be applied to detect the presence of fungal infection and predict the likelihood of the development of disease or allergic response at an
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Genetic Variation Associated with Coeliac Disease

The present invention provides a method of diagnosing coeliac disease, said method comprising analysing a sample of nucleic acid from a human subject to determine the presence or absence of one or more single nucleic polymorphisms (SNPs) in one or more human chromosomal regions selected from the group consisting of Iq31, 2ql l-2ql2, 3p21, 3q25-3q26, 3q28, 6q25 and 12q24.
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A Method for Improving Gene Expression

The present invention is directed to a method for improving gene expression in a host cell comprising a modified protein encoding nucleic acid comprising the steps of assessing the A and T nucleotide content and/or the intrinsic curvature of a wild type protein encoding nucleic acid or mutant thereof, preparing an altered protein encoding nucleic acid with modified A and T nucleotide content and using the altered protein encoding nucleic acid in host cell gene expression systems. The present inv
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Alternatively Transcribed Genes Associated with Memory Consolidation

Methods of identifying gene targets associated with learning and memory and with memory consolidation, as well as to specific nucleic acid molecules that have been discovered to be associated with memory consolidation are provided. Accordingly, the invention also relates to methods of modulating learning and memory function, methods of diagnosing learning and memory disorders, and methods of identifying compounds that modulate learning and memory function via manipulations of the nucleic acid mo
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Modified Thromboxane A2 Receptor Promoter Sequence

The invention provides nucleic acid sequences useful in regulating the transcription of a gene. In particular, the invention relates to a promoter sequence, and variants thereof, that can be used to differentially regulate the transcription of a gene. The present invention, accordingly, provides methods for regulating transcription of a gene, the method comprising providing a gene transcription- regulating polynucleotide in operable association with the gene, optionally within a host cell, where
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Pathway Analysis of Cell Culture Phenotypes and Uses Thereof

The present invention provides methods for systematically identifying genes, proteins and/or related pathways that regulate or indicative of cell phenotypes. The present invention further provides methods for manipulating the identified genes, proteins and/or pathways to engineer improved cell lines and/or to evaluate or select cell lines with desirable phenotypes.
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Methods for Determining a Breeding Value Based on a Plurality of Genetic Markers

The present invention provides a method for determining the individual effect of a plurality of genetic marker alleles on udder health, fertility and/or other health of a plurality of reference bovine subjects. The marker effects are employed in another aspect of the invention for determining a genomic estimated breeding value of a bovine subject based on the genotype of said bovine subject by correlating its genotype with the effect of each individual genetic marker allele on udder health, fert
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A Genetic Marker Test for Brachyspina and Fertility in Cattle

This invention relates to methods for the detection of bovine that are affected by or carriers of Brachyspina (BS). The present invention provides a method for determining whether a bovine is affected by or earner of BS by analyzing its genomic DNA or its RNA. The method can be used to perform marker assisted selection or genomic selection for increased fertility in said bovine.
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A genetic marker test for brachyspina and fertility in cattle

This invention relates to methods for the detection of bovine that are affected by or carriers of Brachyspina (BS). The present invention provides a method for determining whether a bovine is affected by or carrier of BS by analyzing its genomic DNA or its RNA. The method can be used to perform marker assisted selection or genomic selection for increased fertility in said bovine.
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Plant Gene Expression

The present invention relates to a nucleic acid molecule for forming a promoter for regulating transcription of a gene in a plant in anoxic conditions including one or more nucleotide sequences as shown in SEQ. ID Nos. 1 - 14 wherein each of the sequences are spaced apart from each other to define regions for forming regulatory elements for interacting with a transcription factor. Also encompassed by the present invention are uses of this nucleic acid molecule for producing an expression product
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IMMUNOSTIMULATORY siRNA MOLECULES

The present invention relates to a double-stranded siRNA molecule that is capable of silencing gene expression as well as inducing an immune response. The molecule comprises a sense strand and an antisense strand, wherein the antisense strand comprises a first nucleotide sequence that is specifically complementary to mRNA transcribed from a target gene, and the sense strand comprises a second nucleotide sequence that is substantially or perfectly complementary to the antisense strand with the ex
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Xenobiotic Related Induction of Gene Expression

An isolated nucleic acid molecule comprising a nucleotide sequence encoding a transcriptional enhancer of cytochrome P450 (P450) CYP3A4 production or expression, and uses of the nucleic acid molecule for screening compounds for xenobiotic induction of CYP3A4 expression in cells and animals.
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Endocrine Disruptors

A method is provided for assessing the potential of one or more test compounds to disrupt the endocrine system. The method comprises measuring the effect of the test compound(s) on hormone sulphation, oestrogen synthesis, and thyroid hormone and/or growth hormone function. These tests may be carried out sequentially or in parallel. Also provided is a test kit for assessing the endocrine disruption potential of a compound. The kit comprises means for assessing the effect of a compound on hormone
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Methods and Products for Measuring Free Immunoglobulin Light Chain Molecules

The present invention provides improved methods for measuring the ratio of free K immunoglobulin light chain molecules to free ? immunoglobulin light chain molecules in a test sample, using a monoclonal antibody that specifically binds to free K and a monoclonal antibody that specifically binds to free ?. The application also provides specific monoclonal antibodies that can be used in the method of the invention, as well as kits containing said monoclonal antibodies.
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Solubilisation of Membrane Proteins

A method is provided for solubilising a membrane protein. The method is applied to cellular material comprising the membrane protein and an associated membrane lipid. A copolymer of styrene and maleic acid, wherein the styrene: maleic acid ratio is between 1:2 and 10:1, is mixed with the cellular material to cause the copolymer, lipid and protein to form soluble macromolecular assemblies.
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Prognostic Assay for Determining T Cell Response to Hla Antigens and Use Thereof in Field of Tissue Transplantation

The invention provides an in vitro method of determining whether an individual is at risk of, or undergoing, graft damage or rejection of immune origin and/or damage of immune origin to non-graft tissue using polypeptides derived from a major histocompatibility complex (MHC) class I human leukocyte antigen (HLA), such as HLA-A2, and derivatives or analogues thereof.
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Modified Plasminogen Activator Inhibitor Type-1 Molecule and Methods Based Thereon

The present invention relates to a modified plasminogen activator inhibitor type- 1 (PAI-1) molecule that displays an increased in vivo half-life of the active form of PAI- 1, but is deficient in one or more functional activities as compared to the wild-type PAI- 1 protein. The modified PAI-1 molecule that displays an increased half-life further displays at least one of the following funtional characteristics: (i) decreased binding activity to at least one of the following molecules: urokinase p
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Stem Cells Derived from the Carotid Body and Uses Thereof

Adult stem cells obtained from the carotid body, characterized in that they are positive for the phenotypic marker GFAP (glial fibrillary acidic protein) and negative for the phenotypic markers TH (tyrosine hydroxylase) and nestin, are described. These stem cells can undergo proliferation, self-renewal and differentiation to progenitor cells and differentiated cells. Said stem cells, progenitor cells and differentiated cells, expanded by any method, can be used in the treatment of neurodegenerat
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Dendritic Cell Precursor Populations, Dendritic Cell Populations Derived Therefrom and Uses Thereof

Dendritic cell precursor populations, dendritic cell populations derived therefrom, methods for isolating, expanding and using are disclosed.
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Methods and Compositions for Identifying Biomarkers Useful in Characterizing Biological States

The present invention relates to methods, compositions, and kits for identifying biomarkers useful in characterizing biological states. In particular, the invention relates to methods and compositions for molecular characterization of biological states by gene expression profiling. The invention also relates to assessing effects of DNA polymorphisms on regulation of transcription. The biomarkers and polymorphisms identified find use in diagnostic and treatment approaches, e.g., some embodiments
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Small Molecule Intervention for Obesity.

Methods and compositions for activating PLTP gene expression include administering an effective amount of a limonoid.
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Biomarker

Described are gastrointestinal cancer specific biomarkers comprising the nucleic acid sequence of the Engrailed-2 (EN2) gene or the amino acid sequence of the encoded EN2 protein. Also described are uses of the biomarkers in the treatment, diagnosis, monitoring and imaging of gastrointestinal cancer.
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Therapeutic Peptides, Polypeptides Ans Nucleic Acid Sequences

Described are isolated peptides (GLGGGDLSV, SLNESQIKI, LMLPAVLQA and FTAEQLQRL) derived from portions of the Engrailed-2 (EN2) protein. Also described is their use in therapy, in particular in relation to the prevention and treatment of cancer.
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Biomarker ER2 for Gynaecological Cancer

Described are gynaecological cancer specific biomarkers comprising the nucleic acid sequence of the Engrailed-2 (EN2) gene or the amino acid sequence of the encoded EN2 protein. Also described are uses of the biomarkers in the treatment, diagnosis, monitoring and imaging of gynaecological cancer.
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Genetic Markers Predicting Age At Menopause

The present relates to a method of predicting the age at natural menopause in a female individual comprising detecting at least one SNP in a gene selected from the group consisting of CLCA4, KALRN, TOPBP1, the gene for hypothetical protein FLJ16641, VCAN, MCTP1, BAI3, AGR2,LSM5, SEMA3E, CSMD1, CPA6, LINGO2, the gene for hypothetical protein C9orf85, EHMT1, ADARB2, DNA2L, MGMT, SPATA19, the gene for hypothetical protein LOC146167, MSI2, BRSK1, SUV4-20H2, HSPB1, and SCARF2.
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Bisphosphonate Compounds for Chelating Radionuclides

Bisphosphonate compounds for chelating radionuclides are described which have separate bisphosphonate and metal chelating groups joined by a linker, so that the bisphosphonate groups are available to complex to hydroxyapatite in bone, while the metal chelating group binds to the radionuclide. This avoids problems in the prior art, where the bisphosphonate groups are used for both binding functions which compromises the bone-seeking activity of the bisphosphonate groups when they are used to chel
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Product from Starfish

The invention relates to a product capable of having one or more properties selected from; anti-fouling properties, anti-adhesive properties, anti-inflammatory properties, and wherein said product is obtainable from starfish.
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Method Using HLA Epitope Determination

Disclosed herein are methods and kits for characterizing HLA types in terms of epitopes, which is referred to as "epityping". In one aspect there is provided a method for HLA typing, comprising detecting a plurality of epitopes present in one or more HLA molecules in a sample from a subject, wherein each epitope comprises a region of the HLA molecule which can be recognised by an anti-HLA antibody, and thereby determining a set of HLA epitopes characteristic of the HLA type of the subject.
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Mutually Suppressive Gene/inhibitor Combinations for Non-antibiotic Selection of Recombinant Strains

This invention relates to vector sequences that express growth inhibitory levels of essential genes used in combination with inhibitors that target the same gene. An example given is the use of the gene encoding the fatty acid biosynthesis enzyme enoyl-ACP reductase used in combination with the antimicrobial triclosan. The expression level of the enoyl-ACP reductase is sufficient to suppress the toxic effects of triclosan and the triclosan is used at levels that are sufficient to suppress the to
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Use of FFBPL Gene to Identify a Cause of Infertitlity

Fertility problems affect (1 in 10) couples in Western society, making it one of the most common serious health issues. Despite this, little is known about the causes of infertility, and thus patient counselling and treatment are suboptimal. With infertility being such a common problem, identification of any cause would impact on a large number of patients, allowing better counseling, clearer diagnoses and the possibility of making more informed choices (e.g. adoption vs. IVF treatment). The pre
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Process for Providing Embryonic Stem Cells

Embryonic stem sells, particularly human embryonic stem cells are outstandingly sought as subjects of research owing to their capability for reproduction and differentiation in many, if not in all, cell types in a body. Herewith described invention in the application reveals the procedure according to which embryos, development of which has been interrupted, can be used for obtaining embryonic stem cells.
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Modified Stefin a Scaffold Proteins

The invention provides novel scaffold proteins for the display of peptides such as peptide aptamers. The novel scaffold proteins are modifications of Stefin A or STM (a variant of Stefin A) and are useful as scaffold proteins and as display systems.
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Peptide-coated Fibers

The present invention generally relates to peptide-coated fibers, processes of fabricating said fibers, and articles incorporating said fibers. The fibers comprise a coating-receptive fiber and a peptide coating, of 10 micrometers or less and comprises a molecularly self-assembling material, and the peptide coating comprises at least one self-assembled peptide polymer.
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Process of Fabricating Peptide-coated Fibers

The present invention generally relates to peptide-coated fibers, processes of fabricating said fibers, and articles incorporating said fibers. The fibers comprise a coating-receptive fiber and a peptide coating, wherein the coating-receptive fiber has a diameter of 10 micrometers or less, and the peptide coating comprises at least one self-assembled peptide polymer.
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Method for Monitoring the Dehybridi Zation of Double Stranded Nucleic Acid

The present invention relates, in one aspect, to a method for monitoring the dehybridisation of double stranded nucleic acid, comprising the steps of: (a) providing a nucleic acid probe and a target nucleic acid; (b) forming a double stranded nucleic acid of which at least one of the strands of the nucleic acid is in contact with a solid substrate; (c) applying a potential ramp to the solid substrate; and (d) monitoring the dehybridisation of the double stranded nucleic acid across the potential
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Predictive Use of CpG Methylation

Determination of the methylation status of individual selected Cp/G dinucleotides/ CpG groups 5' to the coding region of selected genes, preferably in a perinatal tissue sample such as umbilical cord, can be used to predict future development of diverse phenotypic characteristics, such as indices of body composition indicative of, for example, obesity or low bone mineral content, indices of impaired cardiovascular structure or function, ability to learn and cognitive function, neurobehavourial p
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Tissue Analysis

The present invention provides a method of tissue analysis, disease modelling or drug development comprising the steps of providing a tissue sample comprising cells of at least two different cell types; exposing cells in the tissue sample to an agent ex vivo; exposing the cells to three or more detection means arranged to distinguish between three or more different cell types and/or different cell states; detecting the detection means in order to determine the different cell types and/or differe
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Retention of a Stem Cell Phenotype

A method for promoting the retention of a stem cell phenotype in a population of stem cells. A biocompatible substrate is provided, e.g. in the form of cultureware. The substrate has an arrangement of topographical features arrayed in a pattern based on a notional symmetrical lattice in which the distance between nearest neighbour notional lattice points is between 10 nm and 10 [mu]m, and wherein the topographical features are either located in register with the respective notional lattice point
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Methods of identifying modulators of CFTR/CK2 interaction

A method of identifying a compound capable of modulating CFTR/CK2 interaction and/or CFTR phosphorylation by CK2 comprising exposing CFTR and/or CK2 to a test compound and assessing the effect of the test compound on CFTR/CK2 interaction and/or CFTR phosphorylation by CK2. A method of screening for compounds of use in preventing or treating a secretory state or disorder and/or a cancer and/or cystic fibrosis wherein CFTR and/or CK2 is exposed to a test compound and the effect of the test compoun
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Polypeptides, Polynucleotides and Uses Thereof

A recombinant polynucleotide encoding migrating stimulating factor (MSF) or variants or fragments or derivatives or fusions thereof or fusions of said variants or fragments or derivatives. Reagents are disclosed which can distinguish MSF and fibronectin, and which can distinguish polynucleotides which encode MSF or fibronectin. These reagents are believed to be useful in, for example, diagnosing cancer. MSF or variants or fragments or derivatives or fusions thereof, or fusions of said variants o
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Fluorescence Lifetime and Fluorescence Assays

The invention provides a method for determining a degree of phosphorylation of a substrate, for example a peptide substrate, using a fluorescence probe that acts alone or with another material and has a lifetime that varies when in proximity to a phosphate, the method comprising: causing the fluorescence probe to fluoresce; measuring a time response of the fluorescence, and analysing the fluorescence time response to identify a fluorescence component having a lifetime associated with phosphoryla
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Targeted Modulation of Gene Expression

The present invention relates to a method of modulating gene expression using snoRNA molecules or snoRNA like molecules or fragments, designed to target specific nucleic acid sequences.
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Modulation of Rsk

The present invention relates to specific compounds for use in controlling inflammatory cell function and their potential use in modulating an immune response or inflammation. The present invention also relates to the identification of an alternative RSK pathway in, for example, dendritic cells and the development of assays for the identification of compounds capable of modulating the alternative RSK associated pathway in dendritic cells/macrophage cells and their potential use in enhancing, or
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Treatment of Epithelial Fragility Disorders

The present invention provides compounds which modulate the expression of keratin genes, particularly mutated or defective keratin genes, for treating epithelial fragility disorders such as Meesmann Epithelial Corneal Dystrophy (MECD).
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Biosensor for Analyte Detection

This invention relates to biosensors for detecting analyte molecules, such as nucleic acids, in a sample solution. The biosensors comprise a capture electrode which has probe molecules immobilised at its surface which specifically bind to analyte molecules. The probe molecules are separated on the surface of the capture electrode by spacer molecules and the proportion of probe molecules to spacer molecules is less than 10%. Changes in the electrical properties of the capture electrode surface ar
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Antibodies for identification of murine fragilis extracellular domain and methods for identifying pluripotent cells

We describe two primordial germ cell-specifically expressed genes, GCR1 (Fragilis) and GCR2 (Stella), as well as their fragments, homologues, variants or deriviatives thereof which are markers for primordial germ cells and may be used to identify such cells in cell populations.
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Facile Method for Modifying Retrovirus Envelopes to Purify Retroviruses and/or to Enhance Gene Delivers

Tech ID: 21217 Brief Description Researchers have developed a novel and convenient method to molecularly tune the exterior surface of retroviruses. The glycoproteins on the surface of viruses and virus-producing cells were activated via oxidation and were further bioorthogonally conjugated with desired functional molecules. These modified retroviruses were able to transduce cells at a high efficiency. Full Description By using the method described, the retroviral envelope was shown to
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Improved Cryopreservation of hES and IPS Cells

Isis Project No 4039/4122 Oxford researchers have developed cryopreservation methods for cryopreserving and recovering hES and iPS cells, resulting in improved cell survival and recovery rates. Marketing Opportunity hES cells have become potential sources for many clinical applications, ranging from drug discovery and regenerative medicine to tissue replacement after injury or disease. The market potential of tissue engineering alone is estimated to exceed $10B by 2013 (“Tissue engineerin
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Efficient Cell Cryopreservation Media

Isis Project No 4026 Oxford researchers have developed cell cryopreservation media with well-defined, serum free agents for preserving adult stem cells, human and animal primary cells. Marketing Opportunity The market size of stem cell research products is estimated to be $800M and expanding through double-digit growth each year (Stem cell research products, by Bharat Book Bureau, 2008). The market of cell therapy including stem cell therapy is even greater. Oxford researchers have develope
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RNA Nano-Constructions for Cell Detection and Drug Delivery

BACKGROUND: RNA has been previously demonstrated to assemble into nanoparticles of various shapes and sizes. These nanoparticles are able to carry various non-RNA components, including molecules for specific cell recognition, image detection and therapeutic treatment, making them ideal drug delivery particles. Engineered RNA nanoparticles have been used to target and attack hepatitis B virus, as well as enabling apoptosis in cancer cells. DESCRIPTION: Researchers at UCSB have developed novel
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Novel Agents that act in Steroid-like signaling pathways in Medicine and Agriculture

BACKGROUND: The END-2 protein of the nematode Caenorhabditis elegans is a member of the nuclear hormone receptor family. Classically, nuclear hormone receptors are thought to be localized in the cytoplasm and nucleus of the cell. Steroids or steroid-like molecules, such as retinoids, diffuse across membranes, bind to the appropriate nuclear hormone receptors and cause them to become transcriptional activators. DESCRIPTION: UC researchers have developed new evidence showing that steroid recep
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Methods for Identifying Novel Therapeutics and Diagnostics in the p53 Pathway

BACKGROUND: Current non-surgical cancer therapies rely on the use of compounds or radiation doses that are non-specific for the tumor cells and are highly toxic to humans. Although these treatments are particularly effective against tumor cells, they are also destructive to the surrounding normal cells, which leads to severe side effects. In addition, the non-specificity of these therapies limits their efficacy. Efforts to develop more specific treatments have focused on targeting the defect
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Method and Probes for the Identification of Microbial Genes specifically induced during Host Infection

BACKGROUND: Pathogenic microbes express certain virulence properties only during the infection of a host. Attempts to identify these virulence factors and to develop methods to control infections by these pathogens have been limited by the inability of researchers to accurately mimic the in vivo conditions of the host cell in vitro. A new method, IVET (in vivo expression technology), has recently provided researchers with the means for discovering the genetic source of such virulence factors in
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Novel Antisense Agent

University of California scientists have synthesized novel oligonucleotide analogs for use in antisense and related applications. Unlike ordinary DNA and RNA strands, the U.C. analogs incorporate modifications to the sugar-phosphate backbone that alter electrostatic properties of the strand. These changes enable U.C. analogs to resist attack by nucleases, restriction enzymes, and topoisomerases, thus making the analog much more stable in vivo than existing antisense agents. Also, these changes e
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Tools for the Genetic Manipulation of Fusobacterium Nucleatum

BACKGROUND: Fusobatcerium nucleatum is an anaerobic Gram-negative microorganism that is commonly found in the mouth. For example, Fusobacterium nucleatum is the most frequently isolated pathogen from periodontal disease sites and is believed to be an initiator of periodontal diseases. Moreover, this bacterium is commonly found in abscesses and other infections in the abdomen, blood, chest, lung, sinuses, and female genital tract. Several F. nucleatum proteins that are believed to be associated
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Recombinant Prolactin Antagonist

The hormone prolactin (PRL) exerts various effects in a wide range of physiological processes. Research suggests that PRL may promote cellular proliferation in both breast cancer and prostate cancer cells. University of California researchers have developed a modified PRL analog (S179D) that acts as a strong antagonist to PRL in cell proliferation assays. S179D consists of a wild-type PRL with a single amino acid substitution. This substitution alters the activity of the molecule such that it
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A Method for in Vivo Visualization of Mutated Mouse Cells

BACKGROUND: One method of studying tumors in mice is by using the CRE recombination system to delete or overexpress cancer-control genes in particular tissues at particular times. However, a hurdle in studying tumorogenesis is the difficulty in monitoring the progress of tumors in vivo. Current techniques require sacrifice of the animal followed by in situ work. These methods require the use of large numbers of animals and preclude the possibility of following the progress of a particular tumor
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BPM-3, a Negataive Determinant of Bone Density

BACKGROUND: Bone morphogenetic proteins (BMPs) are members of the TGFb superfamily, a large family of growth factors with varied functions. BMPs induce the formation of cartilage and bone by influencing cell proliferation, differentiation, morphogenesis and apoptosis. BMP signaling activity is regulated by the relative amounts of different BMP family members, and of BMP antagonists, in a given tissue. This regulation in turn controls the development and maintenance of bone, including peak bone d
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EPLIN, a marker for human Cancer

BACKGROUND: EPLIN, a novel gene not previously described encodes a protein expressed in human epithelial cells. EPLIN is of significant importance because its expression is diminished in many forms of human cancers including breast and prostate cancer. Restoring the expression of EPLIN in cancer cells antagonizes oncogenic transformation. DESCRIPTION: UC researchers have discovered a gene encoding EPLIN. EPLIN cDNA has been cloned, completely sequenced, and tested to detect its expression in
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Identification of Sortase

BACKGROUND: Hospital-borne bacterial infections present a major challenge in patient care due to the rising number of strains resistant to multiple antibiotics in recent years. Gram-positive bacteria, such as Staphylococci, Streptococci and Pneumococci, are the most common cause of these often fatal infections and are particularly more difficult to treat in immunocompromised patients. Therefore, there is an urgent need for identifying novel targets sites in these pathogens for the development of
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Identification of Sortase

BACKGROUND: Hospital-borne bacterial infections present a major challenge in patient care due to the rising number of strains resistant to multiple antibiotics in recent years. Gram-positive bacteria, such as Staphylococci, Streptococci and Pneumococci, are the most common cause of these often fatal infections and are particularly more difficult to treat in immunocompromised patients. Therefore, there is an urgent need for identifying novel targets sites in these pathogens for the development of
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Seed Size Regulation using Parent-Specific Hypomethylation

BACKGROUND: In plants, hypomethlyation of genomic DNA helps regulate seed size. At present, antisense MET1 transgenic plants can be used for creating seeds with increased size and weight. However, the dominant nature of MET1 means that a parent's genome, the genome of its gametophytes, and both sets of chromosomes inherited by the seed (maternal and paternal) are all hypomethylated. Hypomethylation of both sets of chromosomes causes reduced fertility and makes it impossible for a plant geneticis
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A Novel Tumor Suppressor Gene

BACKGROUND: Tumor suppressor genes are genes that, when damaged, fail to suppress tumorigenesis. Typically, tumor suppressor genes are genes that, in their wild-type alleles, express proteins that suppress abnormal cellular proliferation. When the gene coding for a tumor suppressor protein is mutated or deleted, the resulting mutant protein or the complete lack of tumor suppressor protein expression may fail to correctly regulate cellular proliferation, and abnormal cellular proliferation may t
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Detection of ATM Mutations and Polymorphisms with MEGA-SSCP

BACKGROUND: Ataxia telangiectasia is an autosomal recessive disorder characterized by progressive cerebellar degeneration, immunodeficiency, growth retardation, premature aging, chromosomal instability, acute sensitivity to ionizing radiation, and a predisposition to cancer, particularly breast cancer. It is caused by mutations in the ATM gene which lead to defects in the DNA repair process and cell cycle control. Given the severity of the disease, there is a need for efficient and accurate diag
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Phosphorylation of Histone H2B as a Marker for Cell Proliferation and Differentiation in Development and Disease

BACKGROUND: Determining the cell cycle state of human cells can provide an important method for early detection of cancerous growth, especially the detection of cells at the G2/M phase of beginning cell division, or mitosis. However, current cell cycle detection methods cannot be correlated unambiguously with mitosis. Many cell cycle regulators are expressed during the entire cell cycle and are activated posttranslationally during cell division by modifications such as phosphorylation, so antibo
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Antibody Fusion Proteins for treating Cancer

University of California researchers have developed a family of antibody fusion proteins with a potential for various therapeutic applications. These inventions represent both novel technologies and products with novel applications. These UC fusion proteins combine an antibody with various cytokines. While these cytokines have been used previously as direct antitumor agents, these antibody-cytokine fusion proteins can be employed in a novel therapeutic strategy. In this role, the fusion prote
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Chemokine and Peptide Derivatives for Would-Healing, Fibrotic Disease Treatment, and Tumor Supression

A University of California researcher has characterized an avian chemokine that acts both directly and as an agent for various growth factors in wound-healing. The gene that encodes this protein is an inducible chemokine gene that is expressed at low levels in tissue of mesenchymal origin, but is expressed at high levels shortly after wounding and persists throughout the period of granulation tissue formation. It is also highly expressed in tissue surrounding tumors induced by Rous-sarcoma virus
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Vectors for the Recombinant Expression of Human Immunoglobulins

Monoclonal antibodies (mAbs) are an essential tool in numerous research, therapeutic, and diagnostic applications, as mAbs can be customized to bind a desired antigen. While this is highly advantageous in generating very high binding specificity, the actual process of producing mAb-producing hybridomas is difficult and time-consuming, and involves mAb protein chains (immunoglobins) derived from animal sources that are not always suitable for use in humans. Researchers at the University of Cal
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Monoclonal Antibodies and Method for Detecting Dioxins and Dibenzofurans

Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are toxic pollutants which pose persistent threats to both the human population and to the biosphere in general. Although the risk posed by these contaminants has ultimately come to be appreciated, there has been no economically viable detection system suitable for either identifying the sites of pollution or the populations at risk. An obvious need exists. Exploiting modern techniques of molecular biology and i
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Polypeptides and Antibodies Associated with HTLV

BACKGROUND: Human T-lymphotropic viruses (HTLV) are part of a family of retroviruses called oncogenic retrovirues. HTLVs were originally identified for their ability to infect human lymphocytes, particularly T cells, resulting in their transformation to lymphomas and leukemias. More recently, they have been associated with "autoimmune-like" neurological and rheumatic disorders, such as Tropical Spastic Paraparesis. HTLVs have a highly conserved X region, containing five overlapping open reading
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Tissue-Specific Gene Inactivation of Beta-1 Integrin

BACKGROUND: Beta-1 integrin is a critical member of the large family of integrin proteins necessary for cell-extracellular matrix adhesion and bi-directional signaling across the cell membrane. Conventional deletion of beta-1 integrin from the mouse genome results in embryonic death soon after implantation of the blastocyst. Thus, analysis of beta-1 integrin function beyond post-implantation embryogenesis is not possible in a classic deletion model. DESCRIPTION: Researchers at the University
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Electronic Detection of Molecular Targets, Including Proteins, Oligonucleotides and other small Molecules

BACKGROUND: While many assays exist for the detection of DNA, RNA, proteins and other molecular targets, most sensors require sample purity and rigorous controls available only under ideal laboratory settings. These constraints significantly dampen the effectiveness of most reported sensing technologies for real world applications. Rapid, accurate and cost-effective sensors that can quickly identify and quantify targets within contaminated samples would provide a critical tool for diagnostics,
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Gene Therapy of Cancer Using a Transmembrane IL-2 Construct

Interleukin 2 (IL-2) is a cytokine protein necessary for production of immune cells that are involved in recognition of aberrant or malignant cells and eradication of tumors. It has been hoped that IL-2 might play a central role in cancer immunotherapy�battling cancer by revving up the immune system. Unfortunately, IL-2 can only be tolerated in small doses by the body. In dose escalation studies, patients treated with high doses of IL-2 showed clinical responses, although severe toxicity exemp
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Rapid Direct Sequence Analysis of Disease Genes by Use of Nested Primers

The present invention provides a method that allows direct sequence analysis of disease genes, in a rapid, accurate, and economical fashion. This method, single condition amplification/internal primer (SCAIP) sequencing, relies on amplification of a large number of exons at a single set of PCR temperatures. Sequencing specificity is achieved by uniform use of a second, nested internal set of sequencing primers. The benefits of this technique are especially apparent in analysis of sequence of lar
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Bioactive Synthetic Analogues of Lysophosphatidic Acid (LPA) for Cell Growth, Muscle Contraction, Platelet Aggregation, etc.

Over thirty new analogues of phosphatidic acid and lysophosphatidic acid (LPA) have been synthesized. Results show that some of these analogues can act as either agonists or antagonists for a variety of receptors, some act as inhibitors of lipid phosphatases, while some potentiate activities of native LPA ligands. Importantly, we have identified LPA analogues that are inactive on one receptor subtype but highly potent agonists for another subtype. <i>Benefits</i> These compounds have signif
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Targeting Genes for Self-Excision in the Germline

Genetic modification is often accomplished through attaching the desired modification sequence to sequences necessary to retain the new genetic material in the organism. These procedures invariably leave genetic material that is no longer needed once selection methods have identified correctly modified organisms. Groups opposing the use and consumption of Genetically Modified Organisms (GMO�s) frequently argue that genes conferring resistance to certain antibiotics and herbicides are in dang
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Gene Therapy of Cancer Using a Transmembrane IL-2 Construct

Interleukin 2 (IL-2) is a cytokine protein necessary for production of immune cells that are involved in recognition of aberrant or malignant cells and eradication of tumors. It has been hoped that IL-2 might play a central role in cancer immunotherapy�battling cancer by revving up the immune system. Unfortunately, IL-2 can only be tolerated in small doses by the body. In dose escalation studies, patients treated with high doses of IL-2 showed clinical responses, although severe toxicity exemp
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Housekeeper Genes for Normalizing Gene Expression Data

Breast cancer is the second leading cause of cancer deaths in women in the U.S. Critical to combating this problem is appropriate diagnosis. Various methods exist for using RT-PCR to evaluate gene expression levels as a marker for different tumor biology. As an internal controls for the amount of RNA in a reaction, housekeeper genes are used which show stable expression across experimental conditions. Finding biological significance in gene expression data can rely heavily on the performance
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Gene Identification Method for use on Microarrays

Microarray gene chips can identify hundreds or even thousands of genes expressed in a given tissue. More importantly, they can be used to examine differences in gene expression between two samples such as in a comparison of healthy versus diseased tissue. While such knowledge is invaluable to the study and diagnosis of pathological processes, a significant drawback for gene microarray studies is that the sheer number of genes that are measured can obscure the optimal set of genes that may be o
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Lineage Restricted Neuron Precursor Stem Cells

Neurodegeneration is a common theme of many nervous system diseases, such as Alzheimer's disease, Parkinson's disease, Amyotrophic Lateral Sclerosis (ALS), head trauma, epilepsy and stroke. These disorders are devastating and expensive, with annual costs currently exceeding several hundred billion dollars in the United States alone, and current treatments are inadequate. Adding to the urgency of the problem is the fact that the incidence of these age-related disorders is increasing rapidly as p
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Novel Technique for 3-Dimensional Multi-layer Growth of Anchorage Dependent Mammalian Cells

Mammalian cells have conventionally been grown as monolayers on solid surfaces of plastic or glass dishes and flasks or on microcarriers. They proliferate and spread to cover the surface, but stop growing when the surface is covered by only a monolayer of cells. This invention discloses a system that supports multilayer growth of anchorage dependent mammalian cells on a variety of extracellular matrices. Cells grown with this method grow at rates comparable to conventional methods, but can re
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Method of Solution-based Scanning for Alterations in a DNA Segment Using a Double-stranded DNA Binding Dye and Fluorescence Melting Profiles

This invention provides a new, solution-based method to determine whether a DNA sequence is identical to a wild-type sequence. The alteration may be a point mutation (such as a transversion or transition) or another mutation (such as an insertion or deletion). This technique uses a double-stranded DNA binding dye and fluorescent melting profiles to detect even a single mutation within a DNA segment. In this invention, a guanine-cytosine (GC)-rich DNA segment is attached to a DNA segment of i
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Assay for Keratin Expression

Keratins play an important role in maintaining cellular integrity and structure. Over 30 highly homologous keratin genes have been found in the human genome. Mutations in or dysregulation of 20 of these genes are known to lead to various human keratin diseases, such as psoriasis and epidermolysis bullosa simplex (EBS). The present invention is a sensitive assay that can specifically and objectively quantify and differentiate the expression level of a panel of highly homologous keratin genes in
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Improved Design of Oligonucleotides and Oligo-Probes for Antisense Technology, Gene Expression Profiling, and Molecular Diagnostics

Many techniques of molecular biology require interaction of oligonucleotide probes (oligo-probes) with DNA or RNA targets as a basic step in their procedure. Efficient hybridization of oligo-probes is critical to the implementation of molecular assays for pathogen detection, genetic screening and diagnosis, and pharmacogenetic analysis. Other important applications include microarray technologies for monitoring gene expression and antisense-mediated gene silencing. In all of these cases, eff
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Two Luciferase Translational Reporter System (2LUCTRS) for Assaying Translational Recoding, Reinitiation, and Internal Initiation

This invention comprises a pair of gene reporter systems that allow quantification of translational recoding, reinitiation and internal initiation of eukaryotes and prokaryotes by cloning corresponding sequences between two independent light-emitting reporters. The invention is configured for measuring expression of two different luciferases. The system also contains a polylinker for insertion of selected genes to be tested. Recoding is determined by monitoring luminescence of one luciferas
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Simple and Highly Efficient in vivo Method for Genome-wide Mutagenesis in Mice

The present invention provides a practical Cre/loxP-based mutagenesis strategy to systematically mutate coding sequence and/or the vast non-genic region of the mouse genome for large-scale functional genomic analysis of the mouse. It was found that under proper guidance, Cre/loxP site-specific recombination could generate large germline deletions and duplications, simply by breeding. Moreover, the same breeding method was able to generate germline translocations between non-homologous chromosom
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Morpholino Antisense Induced Translational Frameshifting

Premature stop codon and framshift mutations account for approximately 25% of all cases of genetic disease. Unfortunately, therapeutic approaches to diseases caused by frameshift and nonsense mutations have been very limited and difficult to implement; as such, there is a need for a method of treating such mutations/diseases. This invention is designed to suppress the phenotypic effects of such mutations by manipulating the process of protein synthesis. Exposing an mRNA to morpholino antisense
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Somatic Mutations in Neurofibromatosis Type 1 Gene in Human Tumors

Neurofibromatosis is a genetic disorder of the nervous system that primarily affects the development and growth of nerve cell tissues. This disorder causes tumors to grow on nerves at any time and location, and can produce abnormalities such as skin changes and bone deformities. The disorder is associated with a significant morbidity and mortality. The current invention is directed to: (a) somatic mutations of the Neurofibromatosis type-1 (NF1) gene which alter ras regulation. (b) methods of scr
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A Novel Vectors for Enhanced Production of Double-Stranded RNA for RNA-Mediated Gene Interference

Double stranded RNA (dsRNA) can specifically inhibit gene function by eliminating gene expression (RNA mediated gene interference, RNAi). Introduction of dsRNA for RNAi can be accomplished by feeding bacteria engineered to produce these dsRNA molecules. This invention describes engineered vectors that effectively produce dsRNA in bacteria. An interesting aspect of the invention is the addition of terminator sequences to the vectors, which enhance inhibition of gene expression by dsRNA. <i>Ben
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Database-Driven Scheduling and Control System and Its Use in a Functional Genomics Laboratory

This technology describes a relational database-driven task engine to control a robotics workstation used in a functional genomics laboratory. The system consists of: 1) a relational database used for recording and executing protocols, 2) a graphic user interface (GUI) for entering tasks into the database using drag-and-drop programming, 3) a set of optional task processors that each use simple rules to determine which task should be executed next, and 4) a series of �sentries� that monitor
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MIG2

A new member of the chemokine family of cytokines has been identified. These chemokines are produced by interferon-gamma stimulated monocytes. Both the human (HuMig) and marine (MuMig) genes have been identified and cloned. These proteins are members of the platelet factor 4/IL-8 cytokine family. Like other members of this family, the Mig proteins are implicated in inflammation and chemotaxis.
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Molecular Cloning of Macrophage Nitric Oxide Synthase

We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like Neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hours after endotoxin treatment. Description (Set) Proposed Use (Set)
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Packaging Recombinant Adeno-Associated Virus Vector

Adeno-associated virus (AAV) vectors may have utility for gene therapy but heretofore a significant obstacle has been the inability to generate sufficient quantifies of such recombinant vectors in amounts that would be clinically useful for human gene therapy application. Stable, helper-free AAV packaging cell lines have been elusive, mainly due to the activities of Rep protein, which down-regulates its own expression and reverses cellular immortalization. This invention provides packaging syste
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Primer Island Transposition: Ty1 integrase-mediated insertion of an artificial transposon into plasmids for the purpose of creating "primer islands" to map and sequence DNA

We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, usin
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Hedgehog Gene

The present invention is based on the seminal discovery that hedgehog proteins undergo autoproteolytic cleavage which results in two separate proteins having distinct functional and structural characteristics. The two polypeptides, referred to as the "N" and "C" fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, are produced after specific cleavage at a G.sup..dwnarw. CF site recognized by the autoproteolytic domain in the native protein. The "C" fragment functions as a
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Reverse Two Hybrid Systems

Methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are usefulin practicing the methods of the invention.
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Invention Relating to Prostatic Cancer

The isolation and characterization of a metastasis tumor suppressor gene KAI1 is disclosed and diagnostic methods and gene therapy approaches utilizing reagents derived from the nucleotide and deduced amino acid sequences of the KAI1 gene are provided.
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A Targeted Gene Delivery System Made of Gelatin and DNA Microsphere Coacervates

Abstract (Set) A target-specific gene delivery system is made of enzymatically degradable gelatin and nucleic acids (DNA or RNA) microparticles with a linking moiety or a targeting ligand attached to the surface. The delivery system can be made by a simple method. Targeting ligands can be attached to the microparticle directly or via a linking moiety. The linkage design allows the attachment of any molecule onto the microparticle surface including antibodies, cell adhesion molecules, hormones a
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Aquaporin 5

A transmembrane water channel protein is isolated in highly purified form from human erythrocytes. An identical protein is also found in kidney tubules. cDNA encoding this protein has been isolated and its amino acid sequence determined. cDNA encoding a transmembrane water channel protein has also been obtained from salivary gland, and an identical protein is found in lacrimal gland, cornea, and lung tissue. The amino acid sequence of the protein has been deduced from the cDNA, and the protein h
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Suppressors of p53 Cancer Mutations & Genetic Assays and Strains using Human TP53

The technology concerns the identification of second-site suppressor mutations that can act as Aglobal suppressors@ to overcome the deleterious effects of common p53 cancer mutations responsible for more than 50% of all cancers. Such suppressor mutations restore function to mutant (tumor-derived) p53 molecules. These mutations were identified by using a p53 yeast assay that assesses p53 function by its ability to bind to a p53 DNA binding site and transactivate the downstream reporter gene URA3
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HIF-1

The purified and characterization of hypoxia-inducible factor 1 (HIF-1) is described. HIF-1 is composed of subunits HIF-1 and HIF-1 . Purified HIF-1 polypeptide, its amino acid sequence and polynucleotide sequence are provided. A HIF-1 variant that dimerizes to HIF-1 producing a nonfunctional HIF-1 complex is described. Methods for the prevention and treatment of hypoxia-related disorders are provided.
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Genetic Testing of Telomere-associated Genes in Dyskeratosis Congenita and Related Disorders

Abstract (Set) Pulmonary Fibrosis involves scarring of the lung where air sacs of the lungs become replaced by fibrotic tissue resulting in the formation of a scar and subsequent irreversible loss of the tissue?s ability to transfer oxygen into the bloodstream. Pulmonary fibrosis with an unknown cause is termed idiopathic. Idiopathic Pulmonary Fibrosis (IPF) is a serious disease effecting approximately 50,000 people in the US annually. There is currently no cure with mortality resulting general
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GENE ENCODING AN INVERTEBRATE a1 CALCIUM CHANNEL SUBUNIT

This invention describes the cloning and sequencing of cDNA strands that encode calcium channel subunits from the fruitfly Drospophila melanogaster. Their temporal and spatial patterns of expression have been determined. The deduced amino acid sequences of these subunits can be used in researching and designing insect specific pesticides. In addition, expression of these clones can be used for screening for new insecticidal compounds and for analysis of mechanism of action. Further, it can be e
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A Cell Injection System Using Carbon Nanotubes

IB-2323, IB-2333 APPLICATIONS OF TECHNOLOGY: Transfection of foreign molecular-scale cargoes into cells (e.g. DNA, RNA, polymers, dendrimers, nanoparticles, etc.) Insertion of probes into cells to observe physical and biochemical interactions. (e.g. quantum dots-probes) ADVANTAGES: Unlike microinjection, nanoneedle injection does not damage the cell membrane Overcomes other limitations associated with microinjection: Eliminates need for a carrier solvent Not limited to larger
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Molecular Fan

Abstract This invention provides a heat dissipation structure for use in optoelectronic devices. More specifically, this technology is an innovative heat dissipation thick/thin organic-inorganic coating containing active molecularly functionalized vibrational lattice modes designed to act as a molecular cooling fan. The molecular fan is powered directly from the heat generated by the device it is cooling, so it is a powerless heat sink with a spaceless nanoassembly. The "molecular fan" is used
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Targeting and Enhancing RNA-RNA Recombination

Abstract This invention concerns an RNA-RNA construct and a method for increasing recombination frequency when the RNA is replicated. A first RNA molecule is derived from a single stranded RNA virus such that it has a non-naturally occurring sequence at least 25 nucleotides long upstream of a set of sequences active in binding an RNA polymerase. The non-naturally occurring sequences are complementary in an antisense orientation to a sequence in a second target RNA molecule also derived from a
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