Detecting and Sorting Methylated Dna Using a Synthetic Nanopore
Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.
TIMP GREGORY [US]; TIMP WINSTON [US]; FEINBERG ANDREW [US]; MIRSAIDOV UTKUR [US]
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