Biotech Patents for Sale or License

Improved Method for Labeling a Microtube

Brief Description: A novel labeling apparatus adapted for microcentrifuge tubes that is durable, easy to use, and does not interfere with laboratory practice. The device comprises a markable panel component and an aperture for frictionally engaging the tube barrel. Need For: Microtubes (a/k/a microcentrifuge tubes) are a commonly used consumable in research, diagnostic, industrial, and forensic laboratories. Labeling microtubes has largely been limited to applying identifying information
...

Machine and or a process that will provide self cleaning advanced hot tubs, baths, and pools, with dispensing functions and automatic scrubbing systems

A self-cleaning advanced hot tub-bath tub, pool with dispensing functions for providing a hot tub with the functionality to automatically cleanse itself and to inject bath oils, perfumes, soaps, bubble baths, medications, and or other items to the hot tub as desired either automatically through memory circuits or manually. A consumer can release bath oils, perfumes, soaps, bubble baths, medications, and or other luxury items to the hot tub as are desired either manually for each individual item
...

Pill for enlarging male genital organ

This is a healthy pill for enlarging the male genital organ.
...

"Liver Dysfunction-Jaundice"

HIPATONE® Why Hipatone and what it can make difference than other related medication MOST IDEAL SUPPLEMENT FOR LIVER FACT SHEET: 1. Safe and effective. 2. Small molecule and easy to digest. 3. Natural source of medicine. 4. No side effects or adverse effect even taken with conventional drug therapy. 5. It helps to improve Liver Cirrhosis conditions, Hepatitis, Jaundice, Indigestion, Hepatomegaly, Loss of Appetite, Hepatoprotective and Immunomodulatory effect. 6. It helps to remove
...

Therapeutic Nucleic Acid-3'–Conjugates

Methods are described for improvement of the serum half life of therapeutic nucleic acids by 3' conjugation to useful target proteins, or other large molecules with useful function. In one embodiment, a 3' A, C or G overhang is added to ds-DNA and the primary amines conjugated using biocompatible bifunctional linkers to proteins. The resulting nucleic acid-3'-conjugates are serum nuclease-resistant and retained in vivo for long periods without rapid kidney clearance. Further, the choice of conju
...

Methods of Producing Intrachain Fluorophore-Quencher FRET-Aptamers and Assays

The present invention describes methods for the production and use of single chain (single-stranded) fluorescence resonance energy transfer ("FRET") DNA or RNA aptamers containing fluorophores (F) and quenchers (Q) at various loci within their structures, such that when its specific matching analyte is bound and the FRET-aptamers are excited by specific wavelengths of light, the fluorescence intensity of the system is modulated (increased or decreased) in proportion to the amount of analyte adde
...

Methods and compositions of DNA ligands (aptamers) for arthropod-borne pathogen detection and prophylaxis or therapy

Specific DNA ligand sequences (aptamers) for binding various arthropod-borne pathogens including arboviruses, rickettsia and parasites are described. Each of these sequences or their linear, two- and three-dimesional linked sequences can function in varying assay and sensor formats with varying degrees of success. Linakge of the whole or partial DNA sequences (putative binding sites) can be used to enhance specificity and affinity towards complex targets, thereby improving assay selectivity a
...

Methods and compositions of nucleic acid ligands (aptamers) for detection of clinical analytes related to human health

Specific DNA sequences (aptamers) for binding various clinically relevant analytes from the human body are described. Each of these sequences or their linear, two- and three-dimesional linked sequences can function in varying assay and sensor formats with varying degrees of success. Linakge of the whole or partial DNA sequences (putative binding sites) can be used to enhance specificity and affinity towards complex targets, thereby improving assay selectivity and sensitivity in many instances
...

Specimen Gathering Device and Method of Use

This is the only DNA gathering device of its kind. It allows the user to simply and effectively gather forensic evidence, without waiting for the sample to dry, or any extra steps to ensure the DNA sample does not become contaminated or degrade. It also can be stored at room temperature, without the worry of degradation, as the SIM Buffer solution preserves the DNA, as well as acting as an anti-microbial and anti-bacterial. Has been used in the market by both police and prison officials. Can
...

Biodegradable Self-Expanding Drug-Eluting Prosthesis

Implantable radially expandable medical prostheses for treatment of vascular diseases through percutaneous deployment.
...

Pathogen derived effectors and their use in enhancing plant resistance to pathogens, insect pests and freezing stress

ABSTRACT Method for producing transgenic plants that are resistant to at least one pathogen and one insect pest are provided. Plants expressing a pathogen-derived effector that are resistant to at least one pathogen, at least one insect pest, and freezing stress are provided. Genes and gene sequences comprising pathogen-derived effectors chosen as to not trigger the hypersensitive response and to be capable of enhancing gene silencing on a given plant are also provided, as well as plants, plan
...

A process for lycopene production by photosynthetic bacteria

Lycopene is a potent antioxidant, anti-inflammatory and anti cancer molecule. It is also helpful for treating a number of conditions like Coronary heart disease, stroke, reduce the risk of cervical, colon, esophageal, rectal, prostate, and stomach cancers, for treating oral submucous fibrosis, gingivitis, age-related macular degeneration, Lycopene also reduce or prevent the side effects, caused by antibiotics. There is great demand exist for lycopene to its beneficial effect. The present inventi
...

Lacritin-syndecan fusion proteins

The present invention relates to methods and compositions useful for the regulation of lacritin, syndecan, and lacritin-syndecan interactions and the signaling pathway downstream of lacritin-syndecan interactions. The invention also relates to regulating lacritin-syndecan interaction to regulate ocular cell survival in response to an insult or injury, in protecting against ocular inflammation, and in promoting ocular wound repair.
...

Hydrogenase Ploypeptide and Methods of Use

Provided herein are polypeptides having hydrogenase activity. The polypeptide may be multimeric, and may have hydrogenase activity of at least 0.05 micromoles H2 produced min-1 mg protein-1. Also provided herein are polynucleotides encoding the polypeptides, genetically modified microbes that include polynucleotides encoding one or more subunits of the multimeric polypeptide, and methods for making and using the polypeptides.
...

Cas6 Polypeptides and Methods of Use

Provided herein are methods for cleaving a target RNA polynucleotide. The target RNA polynucleotide includes a Cas6 recognition domain and a cleavage site, and may be based on a repeat from a CRISPR locus. The methods may be practiced in vivo or in vitro. Also provided are polypeptides that have Cas6 endoribonuclease activity in the presence of a target RNA polynucleotide, and methods for using the polypeptides.
...

Biotin-facilitated transport in gram negative bacteria

Biotinylation of compounds such as peptides and peptidomimetics facilitates illicit transport of the compounds into Gram negative bacteria.
...

Neuronal progenitors from feeder-free human embryonic stem cell culture

The present invention relates to methods for producing feeder cell-free neuroprogenitor cells (preferably adherent) from embryonic stems cells, preferably human embryonic stem cells, the feeder cell-free neuroprogenitor cells, preferably human cells themselves, as well as methods for producing feeder cell-free samples of neuronal cells, preferably adherent human neuronal cells and the feeder cell-free neuronal cells themselves. Pharmaceutical compositions and methods of treating neurodegenerativ
...

Reverse Restriction Fragment Length Polymorphism Assay and Uses Thereof

The present invention presents a Reverse Restriction Fragment Length Polymorphism (RRFLP) method for the detection of the presence of an informative restriction enzyme site in a nucleotide sequence. The method includes digesting a sample with the informative restriction enzyme; performing polymerase chain reaction (PCR) on the digested sample with an oligonucleotide primer pair that flanks the informative restriction enzyme site; determining the Ct value of the sample; comparing the Ct value of
...

Enhancement of Bacillus Thuringiensis Cry Toxicities to Lesser Mealworm Alphitobius Diaperinus

The subject invention relates in part to the discovery that BtBooster (BtB) peptides enhance Bt strain and Cry protein toxicity to the lesser mealworm. The subject invention also relates in part to the discovery that Bt tenebrionis producing Cry3Aa crystals and Bt japonensis BuiBui producing Cry8Ca crystals are insecticidal to A. diperinus larvae (darkling beetle or lesser mealworm). The subject invention also relates in part to the discovery that fragments from cadherins of the western corn roo
...

PROKARYOTIC RNAi-LIKE SYSTEM AND METHODS OF USE

Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5' region and a 3' region. The 5' region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3' region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the pr
...

Pyridinone Hydroxycyclopentyl Carboxamides: HIV Integrase Inhibitors with Therapeutic Applications

New chiral and achiral oxy- substituted cyclopentyl pyridinone diketocarboxamides and their derivatives and methods for their preparations are disclosed. The compounds include tautomers, regioisomers and geometric isomers. These complex carboxamides are designed as inhibitors of HIV replication through inhibition of HIV integrase. The compounds are useful in the prevention or treatment of infection by HIV and in the treatment of AIDS and ARC, either as the compounds, or as pharmaceutically accep
...

Vectors Comprising a Magnum-specific Promoter for Avian Transgenesis

This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced.
...

Recombinant Avian Paramyxovirus Vaccine and Method for Making and Using Thereof

The present invention encompasses engineered APMV compositions or vaccines. The vaccine or composition may be a recombinant APMV composition or vaccine. The present invention encompasses methods for modifying the genome of APMV to produce recombinant APMV; modified APMV prepared by such methods; DNA and protein sequences; and methods for infecting cells and host animals with such recombinant APMV.
...

Modified Peptides Having Toxin-enhancing Effects.

This invention relates in part to modifying BtBooster (BtB) peptides, in part to increase their stability in insect midgut digestive juices. Some preferred embodiments of BtB have removed proteinase cleavage sites resulting in increased stability of the modified BtB in the insect gut, while retaining the ability to enhance B.t. proteins for improved insect control. In some preferred embodiments, the protease-stable BtB is used in combination with B.t. spores and/or crystals comprising a Cry prot
...

Anti-trypanosomal Peptides and Uses Thereof

The present invention provides methods of killing, inhibiting the growth, and/or inhibiting the reproduction of kinetoplastid protozoan with hydrophobic signal sequence peptides and compositions including such hydrophobic signal sequence peptides.
...

Prophylactic and Therapeutic Immunization Against Protozoan Infection and Disease

Polypeptide and polynucleotide vaccines effective to treat or prevent infection of a mammal, such as a dog, a cat, or a human, by a protozoan. Methods of treatment and prevention are also provided, including therapeutic administration of the vaccine to an infected mammal to prevent progression of infection to a chronic debilitating disease state. Preferred embodiments of the polynucleotide vaccine contain nucleotide coding regions that encode polypeptides that are surface-associated or secreted
...

Glycoprotein Cancer Biomarker

The invention relates to glycoproteins having a cancer-specific glycoform. Cancer-specific glycoforms are useful in diagnostics and therapeutics.
...

Aptamers as agonists

Nucleic acid aptamers are described herein which can transduce a signal into cells by crosslinking a cell surface molecule, thereby inducing of one or more biological activities by the cells; in serving as agonists.
...

Antidotes for Agonistic Aptamers

The present invention relates, in general, to aptamers and antidotes therefor and, in particular, to aptamers capable of binding to and activating molecules, such as receptors (e.g., the OX40 receptor) and enzymes. The invention further relates to antidotes to such agonistic aptamers. The invention also relates to compositions comprising, and to methods of using, the agonistic aptamers and the antidotes therefor.
...

Single-cell biosensor for the measurement of GPCR ligands in a test sample

The present invention is related to the detection of GPCR ligands in a test sample by using a single cell biosensor expressing a GPCR. Preferably, the test sample is derived from a biological or environmental sample. This invention may be used to detect the presence of a disease or to detect the presence of a harmful agent in the environment. Included in the present invention is an array of biosensors that detect ligands of various GPCRs.
...

Detection Assay Devices and Methods of Making and Using the Same

An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes:(a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
...

Predicting Responsiveness to Cancer Therapeutics

Provided herein are methods for predicting the responsiveness of a cancer to a chemotherapeutic agent using gene expression profiles. In particular, methods for predicting the responsiveness to 5-fluorouracil, adriamycin, cytotoxan, docetaxol, etoposide, taxol, topotecan, PB kinase inhibitors and Src inhibitors are provided. Methods for developing treatment plans for individuals with cancer are also provided. Kits including gene chips and instructions for predicting responsiveness and computer r
...

Resin Assisted Capture of Cysteine-modified Proteins/peptides and Determination of Presence and Location of Modification

A resin connected by amide bond to pyridyl sulfide or methylthiosulfonate can be conjugated to a post-translationally modified protein/peptide to allow determination of presence and kind and optionally location ofcysteine modification(s).
...

Treating Oncogenic Ras Driven Cancers

Oncogenic Ras-driven cancer is treated with agent by interrupting pathway comprising activation of Akt by oncogenic Ras, activated Akt causing phosphorylation of eNOS at S1177 site of eNOS to provide activated eNOS in cancer cells and activated eNOS causing activation of wildtype Ras by nitrosylation thereof at C118 to provide GTP -bound activated wildtype H and N Ras. L-NAME can be orally administered to interrupt this pathway. Wortmannin can be administered intravenously to interrupt this path
...

VHH Antibody Fragments for Use in the Detection and Treatment of Cancer

The presently disclosed subject matter provides ligands for detecting and imaging cancer cells and tumors, and for guided delivery of an active agent to cancer cells and tumors. In some embodiments the ligands comprise an antibody fragment, wherein the antibody fragment comprises a VHH domain. In some embodiments a composition is provided for targeting of cancer cells or tumors. Also provided are methods for delivery of a composition to a target tissue or tumor in a subject. Also provided are me
...

Characterization of GRP94-ligand Interactions and Purification, Screening, and Therapeutic Methods Relating Thereto

The presently disclosed subject matter discloses characterization of interactions between ligands and Hsp90 proteins, including GRP94, wherein ligand binding to the N-terminal nucleotide binding domain of GRP94 elicits a conformational change that converts the GRP94 from an inactive to an active conformation, and wherein the chaperone and peptide-binding activities of the GRP94 are markedly stimulated. Also disclosed are purification, screening, and therapeutic methods pertaining to the biologic
...

IgG Fc/HIV-gp120/C3d FUSION PROTEIN

PROBLEM TO BE SOLVED: To provide an immunogen for inducing antibodies that neutralize a wide spectrum of HIV primary isolates, and a method for inducing anti-HIV antibodies using the immunogen. ; SOLUTION: There is provided a fusion protein comprising an IgG Fc component, an HIV envelope component, and a C3d component. The IgG Fc component is present in the fusion protein N-terminal to the HIV envelope component. The HIV envelope component is present in the fusion protein N-terminal to the C3d c
...

Hedgehog Signaling and Cancer Stem Cells in Hematopoietic Cell Malignancies

The present disclosure relates generally to methods of treating hematopoietic cell malignancy in a subject by administering to the subject a Hedgehog (Hh) pathway antagonist, such as a Smoothen (Smo) antagonist alone or in combination with an ABL-kinase antagonist. Specifically, the disclosure relates to a method of treating hematopoietic cell malignancy in a subject by administering the Hh pathway antagonist, such as the Smo antagonist cyclopamine, in combination with the ABL-kinase antagonist
...

Photolabile Caged Transition Metal Complexes and Methods of Using the Same

The present invention provides compounds of Formula I: ( I ) along with compositions comprising the same and methods of using the same, such as for photodynamic therapy in the treatment of cancer.
...

COMPOSITIONS AND METHODS USING siRNA MOLECULES FOR TREATMENT OF GLIOMAS

The present invention provides small interfering RNA (siRNA) molecules, compositions containing the molecules, and methods of using the compositions to treat gliomas.
...

Autoantibodies in the Detection and Treatment of Cancer

Methods, kits, and compositions for detecting and/or treating cancer based on detecting and/or administering an antibody, which can optionally be a bispecific antibody, having the immunoreaction characteristics of a cancer-associated autoantibody; an antigen for a cancer-associated autoantibody; or both an antibody having the immunoreaction characteristics of a cancer-associated autoantibody and an antigen for a cancer-associated autoantibody, in or to a subject in need thereof.
...

Modulation of immune response by non-peptide binding stress response polypeptides

A recombinant stress response polypeptide that lacks an antigen binding domain, and methods for using the recombinant stress response polypeptide to elicit an immune response, for example an anti-tumor response, in a subject.
...

Sour Taste Receptor Compositions and Methods

The present invention relates to sour taste receptors and compositions and methods thereof. In particular, the present invention provides assays and methods of screening for ligands specific for sour taste receptors. Additionally, the present invention provides methods for screening for accessory proteins and mutations, polymorphisms and other potential sour taste receptor protein mutations that are associated with disease states, and therapeutic agents, ligands, and modulators of such proteins.
...

Predicting Cancer Risk and Treatment Success

Provided herein are methods for predicting the likelihood of recurrence of a cancer using gene expression profiles. Methods for developing treatment plans for individuals with cancer are also provided. Kits including gene chips and instructions for predicting recurrence of a cancer and computer readable media comprising recurrence information are also provided.
...

Cross-species Chimeric RNA Molecules

Methods to reduce the expression of at least one gene of an intracellular pathogen in a host cell are provided, wherein the method comprises introducing into a host cell a heterologous polynucleotide comprising or encoding a miRNA that fuses with at least one mRNA of the pathogen and reduces the translation of the mRNA. Pharmaceutical compositions comprising such polynucleotides, wherein the reduction in pathogenic gene expression leads to an inhibition of growth or differentiation of the pathog
...

Methods of Inhibiting Cancer Cell Growth with Hdac Inhibitors and Methods of Screening for HDAC10 Inhibitors

Methods of inhibiting cancer cell growth using HDAC10 inhibitors are provided. Methods of treating cancer in a subject using HDAC10 inhibitors are also provided. In certain embodiments, at least one second inhibitor selected from an autophagy inhibitor, an AMPK inhibitor, and methylpyruvate is also used in the methods. Dose packs comprising HDAC10 inhibitors and at least one second inhibitor are provided. Methods of identifying HDAC10 inhibitors are also provided.
...

Monoclonal Antibody Production in B Cells and Uses Therof

The presently disclosed subject matter provides methods of inhibiting a host innate response to activator-mediated proliferative signals in a primary B cell. In some embodiments a method is provided for immortalized primary B cells. In some embodiments a method is provided for increasing efficiency of EBV transformation of primary B cells. In some embodiments a method is provided for increasing proliferation of primary B cells in culture. In some embodiments a method is provided for producing a
...

Affinity Displacement Molecular Separation

Provided herein are methods for identifying molecules that compete with a ligand for binding to a protein. The method comprises contacting a ligand with a proteome to allow protein members of the proteome to bind with the ligand, followed by the removal of the unbound ligand. At least one test molecule is added to the mixture and after passing the mixture through an ultrafiltration membrane, the filtrate is assayed for the displaced ligand. The presence of the ligand within the filtrate indicate
...

Delivery Method

The present invention relates, in general, to RNA silencing and, in particular, to a method of effecting targeted delivery of an RNA silencing moiety using a targeting moiety that binds to a cell surface receptor and mediates internalization of the RNA silencing moiety to be accessible to Dicer. Also provided is a chimeric nucleic acid molecule comprised of a targeting moiety and an RNA silencing moiety, wherein the targeting moiety is an aptamer and the RNA silencing moiety comprises a Dicer su
...

Combination Therapy in the Treatment of Cancer

Disclosed herein is a method of treating a tumor by administering to the subject a treatment effective amount of a therapeutic antibody and an alkylating agent.
...

Biodegradable Copolymer Comprising Amylopectin Or Glycogen Suitable for Delivering Nucleic Acid Materials Into Cells

The invention is related to a biodegradable copolymer, which is suitable for delivering nucleic acid materials into cells, and which comprises a amylopectin or glycogen and at least one cationic polymer or cationic monomer having amino functions, which is bound to the amylopectin or glycogen by ester linkage between at least one free hydroxyl group of the amylopectin or glycogen and at least one amino group of the cationic polymer or cationic monomer forming an -O-C=O-NH- bond. The biodegradable
...

Biodegradable Composite System and the Use Thereof

The invention relates to a composite system of at least one biodegradable blend containing at least one biodegradable Blockcopolyesterurethan (PEU) and at least one polyhydroxyalkanoate (PHA), optionally a filler made of a polysaccharide and / or its derivatives, and possibly other biocompatible additives. Such composite systems are used for the production of molded, extruded or molded parts.Furthermore, the invention uses the composite system.
...

Formulation for Inducing Broadly Reactive Neutralizing Anti-HIV Antibodies

The present invention relates in general, to a formulation suitable for use in inducing anti-HIV-1 antibodies, and, in particular, to a formulation comprising Toll Like Receptor (TLR) agonists with HIV-1 gp41 membrane proximal external region (MPER) peptide-liposome conjugates for induction of broadly reactive anti-HIV-1 antibodies. The invention also relates to methods of inducing neutralizing anti-HIV-1 antibodies using such formulations.
...

Formulation

The present invention relates, in general, to a formulation suitable for use in inducing anti-HIV-1 antibodies and, in particular, to a formulation comprising a prehairpin intermediate form of HIV-1 envelope gp41 linked to a liposome. The invention also relates to methods of inducing broadly neutralizing anti-HIV-1 antibodies using such a formulation.
...

Methods of Making Cell Sheets, Tissue Sheets and Tissue Engineered Blood Vessels

Methods of making cell sheets, tissue sheets and tissue-engineered blood vessels are provided. The methods include culturing cells on a nanoimprinted coated scaffold under hypoxic conditions to form an aligned cell sheet and then removing the aligned cell sheet from the scaffold. The cell sheets, tissue sheets and tissue-engineered blood vessels made the methods are also disclosed. The cell sheets, tissue sheets and tissue-engineered blood vessels may be implanted in subjects to treat a variety
...

Mouse Models

The present invention relates, in general, to animal models suitable for testing candidate immunogens and, in particular, to knock-in mice expressing heavy and light chains of membrane proximal external region (MPER) HIV-I broadly neutralizing antibodies and to methods of screening candidate immunogens using same.
...

Non-invasive Energy Upconversion Methods and Systems for In-situ Photobiomodulation

Products, compositions, systems, and methods for modifying a target structure which mediates or is associated with a biological activity, including treatment of conditions, disorders, or diseases mediated by or associated with a target structure, such as a virus, cell, subcellular structure or extracellular structure. The methods may be performed in situ in a non-invasive manner by placing a nanoparticle having a metallic shell on at least a fraction of a surface in a vicinity of a target struct
...

Modulators of Odorant Receptors

The present invention relates to polypeptides capable of promoting odorant receptor cell surface localization and odorant receptor functional expression. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for odorant receptor accessory protein polymorphisms and mutations associated with disease states, as well as methods of screening for therapeutic agents, ligands, a
...

Methods for Loading Contrast Agents Into a Liposome

The invention comprises compositions and methods for loading both magnetic resonance contrast agents and therapeutic agents into liposomes, such as low temperature sensitive liposomes (LTSLs). In certain embodiments, a passive technique is used to load the liposomes. In other embodiments, an active technique is used to load the liposomes. In further embodiments, a magnetic resonance contrast agent and Doxorubicin are loaded into the liposomes. The liposome compositions have higher contrast-agent
...

Temperature-sensitive Liposomal Preparation

PROBLEM TO BE SOLVED: To provide a liposome useful in methods of administering active agents using mild hyperthermic conditions. ; SOLUTION: There is provided the liposome containing an active agent and has a lipid bilayer membrane comprising phospholipid and lysolipid, wherein the phospholipid is a primary lipid source of the lipid bilayer membrane, and the lysolipid is contained in the bilayer membrane in an amount sufficient to increase the percentage of the active agent released at a phase t
...

Therapeutic Agents Comprising Elastin-like Peptides

The present invention provides therapeutic agents and compositions comprising elastin-like peptides (ELPs) and therapeutic proteins. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist, insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia,
...

Chaperone-assisted Protein Expression and Methods of Use

The present disclosure provides methods of utilizing chaperone proteins for the production of active protein such as those encoded by the genes of natural biosynthetic clusters. The methods provided herein have applicability for a wide variety of genes ranging from small fatty acid biosynthetic genes to large non-ribosomal peptide synthetase genes.
...

Peripheral Blood Biomarkers for Idiopathic Interstitial Pneumonia and Methods of Use

The present invention provides methods for diagnosing several types of diseases. Specifically, the present disclosure provides a panel of diagnostic genes, the differential expression of whose mRNAs or proteins in the sample of a subject indicates the presence of the disease in the subject. The methods involve extracting mRNAs or proteins from the sample and performing gene expression profiling assays such as microarray assay, RT-PCR oligonucleotide binding array, quantitative RT-PCR assay, prot
...

Methods of Manipulating Cell Signaling

The disclosure provides for methods of monitoring and/or modulating cell signaling, and specifically, polynucleotides, polypeptides, cells, assays, genetically modified non-human animals, and methods that incorporate a light-generating protein and a light-transducing protein that can be used to manipulate, monitor, and study cell signaling in various cell types.
...

N-terminal Dimerization Methods with Bis-amindino Acid-and Bis-thioimidate Derivatives

The invention provides high-yield protein dimerization methods using highly reactive bis-thioimidates that may be used in the manufacture of a highly potent anti-cancer peptide dimers.
...

HIV-1 Antibodies

The present invention relates, in general, to HIV-1 specific antibodies and, in particular, to broadly neutralizing HIV-1 specific antibodies that target the gp41 membrane-proximal external region (MPER). The present invention also relates to a cell culture system, more specifically, to a method of rendering chronic lymphocytic leukemia B-cells immortal and to a method of isolating anti-viral antibodies from clones of such cells.
...

Inhibition of Endosomal Toll-like Receptor Activation

The present invention relates, in general, to pattern-recognition receptors (PRRs), including toll-like receptors (TLRs), and, in particular, to a method of inhibiting nucleic acid-induced activation of, for example, endosomal TLRs using an agent that binds to the nucleic acid ("nucleic acid binding agent"), preferably, in a manner that is independent of the nucleotide sequence, the chemistry (e.g., DNA or RNA, with or without base or sugar modifications) and/or the structure (e.g., double-stran
...

Compositions, Methods and Kits for Eliciting an Immune Response

The present invention relates to compositions, methods, and kits for eliciting an immune response to at least one CMV antigen expressed by a cancer cell, in particular for treating and preventing cancer. CMV determination methods, compositions, and kits also are provided.
...

Conjugates prepared with aggregate-free proteins

A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or ac
...

Tissue Engineering

The present invention relates, in general, to tissue engineering and, in particular, to a method of extending the lifespan of cells suitable for use in vascular engineering. The present invention relates to a method of producing vascular grafts. The method comprises introducing into cells an expression vector that comprises a nucleic acid sequence encoding a lifespan extending and/or immortalizing gene, or functional portion or variant thereof, under conditions such that the sequence is expre
...

Methods for treating cancers and pathogen infections using antigen-presenting cells loaded with RNA

The present invention relates to methods for producing an RNA-loaded antigen presenting cell (APC), said method comprising introducing into an antigen presenting cell in vitro RNA encoding an immunomodulator. The present invention also relates to RNA-loaded APCs obtainable by such methods.
...

Peg-urate Oxidase Conjugates and Use Thereof

A naturally occurring or recombinant urate oxidase (uricase) covalently coupled to poly(ethylene glycol) or poly(ethylene oxide) (both referred to as PEG), wherein an average of 2 to 10 strands of PEG are conjugated to each uricase subunit and the PEG has an average molecular weight between about 5 kDa and 100 kDa. The resulting PEG-uricase conjugates are substantially non-immunogenic and retain at least 75% of the uricolytic activity of the unmodified enzyme.
...

Compositions and Methods for Enhancing Odorant Receptor Activity

The present invention relates to polypeptides capable of modulating odorant receptor activation. In particular, the present invention provides polypeptides (e.g., type 3 muscarinic actetylcholine receptor M3) capable of enhancing odorant receptor activation. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for polypeptide polymorphisms and mutations associated with
...

Microrna and Use Thereof in Identification of B Cell Malignancies

Disclosed are nucleic acid sequences, including microRNA sequences and cDNA sequences, as well as vectors, DNA libraries, microarrays, and recombinant cells comprising the nucleic acid sequences described herein. Methods of determining the B cell stage from which a B cell malignancy is derived. Methods of identifying B cell malignancies are also provided. Methods of diagnosing B cell malignancies are provided. Such methods comprise, in certain embodiments, detecting one or more microRNAs or cDNA
...

Method of Inducing an Anti-viral Immune Response

The present invention relates to a method of inducing an anti-viral immune response. The method comprises administering to a patient in need thereof an antigen that induces the production of antibodies that, upon binding to a cell surface target, result in the production of chemokines that inhibit viral infection.
...

Hematopoietic Stem Cell Growth Factor

The present invention relates, in general, to stem cells and, in particular, to a hematopoietic stem cell (HSC) growth factor and to methods of using same.
...

RNA aptamers and methods for identifying the same

RNA aptamers and methods for identifying the same are disclosed. The RNA aptamers selectively bind coagulation factors, E2F family members, Ang1 or Ang2, and therapeutic and other uses for the RNA aptamers are also disclosed.
...

Anti-CD19 Antibodies and Uses in B Cell Disorders

The invention relates to immunotherapeutic compositions and methods for the treatment of B cell diseases and disorders in human subjects, such as, but not limited to, B cell malignancies and autoimmune diseases and disorders, using therapeutic antibodies that bind to the human CD19 antigen and that preferably mediate human ADCC. The present invention relates to pharmaceutical compositions comprising human or humanized anti-CD19 antibodies of the IgG1 or IgG3 human isotype. The present invention
...

Integrated Droplet Actuator for Gel Electrophoresis and Molecular Analysis

The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to
...

Composition and Methods for Eliciting an Immune Response

The present invention relates to compositions, methods, and kits for eliciting an immune response, in particular for eliciting an immune response to at least one antigen expressed by a cancer cell, in particular for treating and preventing cancer.
...

Urate Oxidase

The present invention relates, in general, to urate oxidase (uricase) proteins and nucleic acid molecules encoding same. In particular, the invention relates to uricase proteins which are particularly useful as, for example, intermediates for making improved modified uricase proteins with reduced immunogenicity and increased bioavailability.
...

Viral Microrna

The present invention relates, in general, to micro RN As and. in particular, to viral microRNAs expressed by Herpes Simplex Vims 1 (HSV-1) or Herpes Simplex Virus 2 (HSV-2), to agents that inhibit such microRNAs and to methods of treatment based on the use of such agents.
...

Trimodal Cancer Therapy

Cancers are treated with three types of agents: a chemotherapeutic agent which induces lymphopenia; an inhibitory antibody to a surface marker on Treg cells; and an anti-cancer vaccine. This combination may lead to enhanced immune responses despite lymphodepletion.
...

Regulatory B Cells and their Uses

The present invention relates to a phenotypically distinct CD1dhigh CD5+ B cell subset that regulates T cell mediated inflammatory responses through the secretion of interleukin-10 (IL-IO). The invention also relates to the use of these IL-IO producing regulatory B cells in the manipulation of immune and inflammatory responses, and in the treatment of disease. Therapeutic approaches involving adoptive transfer of these regulatory B cells, or expansion of their endogenous levels for controlling a
...

Indoline Scaffold Shp-2 Inhibitors and Cancer Treatment Method

The subject invention concerns methods and compounds for inhibiting Shp2. In one embodiment, a compound of the invention has a chemical structure as shown in formula I or II: wherein X, Y, and Z are independently N or S; R1 is cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, any of which can be optionally substituted with one or more of halogen; alkyl; heteroalkyl; -COOH; -C(R3)3, wherein R3 can independently be any of -H or halogen; or -OR4, wherein R4 can be any of H, alkyl. or heteroalkyl;
...

Proteasome Inhibitors for Selectively Inducing Apoptosis in Cancer Cells

The subject invention concerns compounds having activity as inhibitors of proteasomes and methods of using the subject compounds. In one embodiment, a compound of the invention has the chemical structure shown in Formula (I), wherein R1 is an organic cyclic ring structure bonded to a sulfonamide structure; R2 is H, halogen, alkyl, -NR6R7, or heteroalkyl; R3 is H, halogen, -OH, -O-alkyl, alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, -NO2, -NH2 or substituted amines; R4 is H, alkyl, heter
...

Substrate-mimetic Akt Inhibitor

Disclosed herein is a species of peptide and non-peptide inhibitors of Akt, an oncogenic protein. Beginning with a residue of Akt target substrate GSK-3, the functional domains of the GSK-3 residue were characterized. Functionally homologous non-peptide groups were substituted for the amino acids of the GSK-3 creating a hybrid peptide-non-peptide and non-peptide compounds capable of binding to Akt. The non-peptide compounds show increased stability and rigidity compared to peptide counterparts a
...

Inhibition of Shp2/PTPn11 Protein Tyrosine Phosphatase By Nsc-117199 and Analogs

Protein tyrosine phosphatase (PTP) Shp2 is a non-receptor PTP that involved in cell signaling and regulation of cell proliferation, differentiation, and migration. Shp2 mediates activation of kinases that are involved in the pathogenesis of human carcinoma. NSC-117199 was identified as a porent inhibitor of the protein tyrosine phosphatase (PTPa) Shp2. A focused library of analogs incorporating an isatin scaffold was designed and evaluated for inhibition of Shp2 and Shp1 PTP activities. Several
...

Bivm (basic, Immunoglobulin-like Variable Motif-containing) Gene, Transcriptional Products, and Uses Thereof

The subject invention provides polynucleotide sequences, designated BIVM, and transcriptional/translational products obtained from the polynucleotide sequences of the invention. The subject invention also provides polynucleotide and polypeptide sequences provided by SEQ ID NOs: 1-28. Also provided are methods of detecting the presence of BIVM nucleic acids or polypeptides in samples suspected of containing BIVM genes, BIVM transcriptional products, or BIVM translational products. These methods a
...

STAT3 Agonists and Antagonists and Therapeutic Uses Thereof

The present invention relates to methods for modulating, i.e., agonizing or antagonizing, Stat3 (Signal Transducer and Activator of Transcription3) signaling activity for use in gene therapy. Inhibition and/or activation of Stat3 signaling is an effective approach to modulate angiogenesis and the immune response for treatment and/or prevention of inflammation, infection, inflammation, immune disorders, and ischemia.
...

Method of Selectively Inhibiting PKCiota

PKCiota inhibitor1 H-imidazole-4-carboxamide, 5-amino-1 -[2,3-dihydroxy-4-[(phosphonooxy) methyl] cyclopentyl] -,[1 R-(1a, 2ss, 3 ss, 4 a)], (ICA-1 ), targets a unique sequence (amino acid residues 469-475) in the catalytic domain of PKCiota and inhibits PKCiota activity. The data shows suprising and unexpected ability of ICA-1 to slectively inhibit the proliferation of cells that overexpress PKCiota.
...

Ship Inhibition to Direct Hematopoietic Stem Cells and Induce Extramedullary Hematopoiesis

The present invention concerns inhibition of SHIP expression and/or function in mammalian subjects to direct HSC from the bone marrow to other sites in the body (such as the spleen and/or liver), where they then re-establish blood cell production. The method of the invention can be employed, for example, to rescue or enhance blood cell recovery during bone marrow impairment due to bone-seeking radioisotope exposure, chemotherapy, radiotherapy, infection, or genetic or congenital defects. Prefera
...

Passive Electric Field Focus System for in Vivo and in Vitro Applications

The present invention provides a system and method of directing, focusing, or concentrating electrical charges within a defined electric field so that these charges can be used to exert forces on cells and tissues in vivo and/or cell cultures in vitro. The present invention reduces and/or eliminates the damage at a target site that would normally be caused by an electrode that acts as a current source or sink to accomplish the same task.
...

Methods and Compositions for Reducing Activity of the Atrial Natriuretic Peptide Receptor and for Treatment of Diseases

Methods, compositions and devices are provided by the present invention for reducing activity of a natriuretic peptide receptor and other signals. Therapeutic treatments are provided by use of polynucleotides encoding a natriuretic peptide or by regulating the expression of natriuretic peptide receptor, such as NPRA and NPRC, or combinations of these therapies. Routes used for delivering polynucleotides encoding a natriuretic peptide, or, for example, siRNA that down regulates natriuretic peptid
...

Method and Composition for Modulating Canonical Wnt Pathway Using Folate and Inositol

The canonical Wnt signaling pathway is implicated in many disorders including neural tube defects, limb malformations, and heart defects, developmental disorders associated with alcohol exposure (fetal alcohol syndrome) or exposure to bipolar medications (i.e. lithium), wound healing, and Alzheimer's disease. Elevated plasma homocysteine (HCy), which results from folate (folic acid, FA) deficiency, the mood-stabilizing drug lithium (Li), and alcohol (ethanol) are linked to the induction of human
...

Hyperactive Variants of 5-aminolevulinate Synthase and Methods of Use

The rate of porphyrin biosynthesis in mammals is controlled by the activity of the pyridoxal 5'-phosphate-dependent enzyme 5-aminolevulinate synthase. Assuming the turnover in this enzyme is controlled by conformational dynamics at a highly conserved active site loop, a variant library was constructed by targeting imperfectly conserved non-catalytic loop residues and the effects on product and porphyrin production were examined. Functional loop variants of the enzyme were tested for porphyrin fl
...

Alpha-helix Mimetic Using a 2,5-oligopyrimidine Scaffold

Alpha-helix mimetics and associated methods of making are provided. These compounds are constructed using a 2,5-oligopyrimidine scaffold. The semi-rigid scaffold holds individual side chain-like residues in orientations that mimic the orientations of side chain residues of an ?-helical protein domain. The new scaffold is easier to make than previous scaffolds and has much more favorable physical properties than previous alpha-helix mimics. The amphiphilic alpha-helix mimetics have application fo
...

Growth Factor Binding Molecules

Growth factor binding molecules having a plurality of peptide loops attached to a non-peptide organic scaffold, preferably having pseudo-six amino acid peptide loops with four amino acid sidechains. The growth factor binding molecules specifically bind various growth factors and are suitable for treating a subject having tumors or restinosis. In one embodiment a platelet-derived growth factor binding molecule is disclosed that is used to inhibit tumor growth and angiogenesis in solid tumors.
...

Volume Exclusion Agent to Enhance Formation of Extracelluar Matrix

A method of enhancing the formation of extracellular matrix in culture. Cells in culture secrete most of the collagen into the media as unprocessed procollagen, i.e., the cells do not convert procollagen to collagen. In contrast, normal extracellular matrix deposition involves procollagen processing to collagen, fibril assembly and deposition into the cell layer to form a collagenous extracellular matrix. The addition of certain growth factors and the addition of a thin layer of a certain volume
...

Inhibition of Cell Proliferation

Compounds of formula (I) and (II) are provided as modulators of Rb:Raf-1 interactions which are potent, selective disruptors of Rb:Raf-1 binding. Therapeutic methods of using the compounds, for example for treating or ameliorating a cell proliferation disorder such as cancer, are provided.
...

Akt and Clusterin As Biomarkers of Chemotherapeutic Responsiveness

Compositions and methods for identifying cancers resistant to treatment due to the presence of elevated pAKT levels and/or overexpression of AKT. The methods comprise the detection of active phosphorylated (p)AKT in a cell or sample of cells. The methods can further include treating the patient with one or more inhibitors of AKT or clusterin responsive to the detection of binding of the antibody to the activated AKT protein or detection of overexpression of AKT. It is found that treatment with A
...

Materials and Methods for Preventing Or Treating Neurodegenerative Conditions Associated with Abeta Peptide Accumulation

The subject invention concerns methods for preventing and/or treating neurodegenerative conditions associated with Abeta peptide accumulation in neural tissue in a human or animal. The subject invention also concerns methods for preventing or treating Alzheimer's disease-like neuropathology in a person or animal having trisomy 21 (Down's syndrome). In one embodiment, a method of the invention comprises administering a therapeutically effective amount of a compound that inhibits function or activ
...

Method of Activating Nk Cells

The present invention provides a method of mediating hypo-activation of NK cells. Methods of the present invention comprise administering to a subject an effective amount of broad acting phosphotase inhibitor, such as sodium orthovanadate, SHP specific inhibitor. In one embodiment, the SHP specific inhibitor is NSC119910.
...

Method of Modulating Ship Activity

A method of treating or preventing an immune disorder, such as graft versus host disease, in a subject. The method includes the administering a SHIP1 inhibitor, such as 3a-aminocholestane, to a subject in need of treatment. Thus, SHIP1 inhibitors taught herein represent a novel class of small molecules that have the potential to enhance allogeneic transplantation, boost innate immunity and improve the treatment of hematologic malignancies.
...

Cytotoxin Compound and Method of Isolation

The present invention concerns compounds derived from tunicates of the species Synoicum adareanum, as well as to pharmaceutical compositions comprising these compounds and methods of use. Extracts from tunicates show selective toxicity against several different cancer cell lines in the NCI 60 cell line panel. These compounds are useful in the effective treatment of cancers, particularly malignant melanomas, colon cancer, and renal cancer cell lines.
...

Immunotherapy for Treatment of Amyloid-Related Disorders Using Encapsulated Beta-Amyloid Peptides

The present invention relates to the field of polymer chemistry and more particularly to encapsulated peptides and uses thereof.
...

Human Mast Cell Line and Uses Thereof

The subject invention pertains to the development of a novel human mast cell line, USF-MC1. USF-MC1 is a mast cell precursor present in human umbilical cord blood (HUCB) that may be sustained in culture in the absence of exogenous cytokines to serve as a convenient experimental model of human mast cell activation. The SCF-independent human mast cell line USF-MC1 responds to IgE-mediated and IgE -independent stimuli in a way comparable to that of LAD2. USF-MC1 cells are useful for investigation o
...

Indoline Scaffold Shp-2 Inhibitors and Method of Treating Cancer

Protein tyrosine phosphatase (PTP) Shp2 is a non-receptor PTP that involved in cell signaling and regulation of cell proliferation, differentiation, and migration. Shp2 mediates activation of kinases that are involved in the pathogenesis of human carcinoma. A high throughput screen identified compounds that inhibit the PTP Shp2. Several compounds were identified that selectively inhibit Shp2 over Shp1 with low to sub-micromolar activity. Also disclosed are methods of inhibiting a protein tyrosin
...

Cancer Treatment Using Natriuretic Peptides

The present invention includes a method of utilizing four peptide hormones to inhibit the growth of cancer(s). A dramatic decrease in the number of human pancreatic adenocarcinoma cells (i.e., the type of cancer with the highest mortality, with patients only surviving four months) was observed responsive to treatment. The application of the invention would be to utilize one or more of these peptide hormones alone and/or in combination to treat cancer. The ability of these peptide hormones to dec
...

Dendroaspis Natriuretic Peptide for Treatment of Cancer

Disclosed is a method of inhibiting the growth of a cancer cell using Dendroaspis natriuretic peptide (DNP), isolated from the Green Mamba snake venom with similar structure to ANP, with or without four cardiac natriuretic peptides i.e., atrial natriuretic peptide (ANP), vessel dilator, long acting natriuretic peptide (LANP), and kaliuretic peptide. Dose-response curves revealed a significant (p<0.0001) decrease in human glioblastoma cells with each ten-fold increase in concentration from 1 [mu]
...

Inhibition of SHIP to Enhance Stem Cell Harvest and Transplantation

The instant invention teaches the inhibition of SHIP expression, or function for the increased efficacy of autologous stem cell transplants. In another embodiment, interference with SHIP function can be used to temporarily expand and mobilize the hematopoietic stem cell compartment to assist with leukapheresis, to promote hematopoietic recovery after myeloablation treatments, to deplete target stem cell clones (such as leukemic clones and other tumor stem cell types), and to deplete, or damage,
...

Antiviral Activity of Cyclopentene Nitro-ester and Derivatives

Disclosed is a method of synthesizing new optically pure heterocyclic compounds using Pd(0) catalyzed intramolecular cyclizations. Analogs of cyclopentanes, like isoxazoline-2-oxide and furan, with similar framework to the cyclopentanes act as anti-HIV and anticancer agents which opens a whole new field for application of these compounds. Starting from a meso-diol, optically pure compounds were prepared without utilizing chiral ligands at any stage of the synthesis. The stereochemical outcome of
...

SHIP-Deficiency to Increase Megakaryocyte Progenitor Production

The invention concerns a method for increasing megakaryocyte and megakaryocyte progenitor numbers in vitro or in vivo by suppressing SH2-containing inositol-5-phosphatase (SHIP) function in megakaryocytes or megakaryocyte progenitors expressing the SHIP gene. SHIP function can be suppressed by administering an interfering RNA, or other SHIP inhibitor, to the megakaryocytes or megakaryocyte progenitors in vitro or in vivo.
...

Platinum Complexes and Methods of Use

The subject invention concerns platinum complexes that exhibit antitumor cell and/or antiparasitic activity. The subject invention also concerns the use of platinum complexes of the invention to treat oncological and inflammatory disorders. The platinum complexes of the invention can also be used to treat or prevent infection by a virus or a bacterial or parasitic organism in vivo or in vitro.
...

Methods for Inhibiting Tumor Cell Proliferation

The subject invention concerns methods for inhibition of STAT biological functions using platinum complexes.
...

Predictive Value of Nuclear Excision Repair Genes for Cancer Survival

Patients who have their cancer surgically removed are at risk for recurrence, even though currently free of disease. Lung cancer patients with higher activity of a nuclear excision repair gene called ERCC1 were at a lower risk for recurrence than patients with lower activity of ERCC1. Hence, it is possible to predict which patients are at a higher risk for recurrence after resection. This information can be used to design treatment strategies for patients determined to be at a higher risk for re
...

Lysophospholipids as biomarkers of ovarian cancer

A method of using a bioactive lysophospholipid (LL) as a biomarker for detecting the presence and recurrence of ovarian cancer. Subspecies of LL, such as lysophosphatidic acid (LPA), lysophosphatidylinositol (LPI), lysophosphatidylcholine (LPC), and lysosphingolipid sphinsosine-1-phosphate (S1P), are used alone or in conjunction to increase the specificity and sensitivity of the assay.
...

DNA Biochip and methods of use

The subject invention concerns materials and methods for detecting nucleic acid sequences. One aspect of the invention concerns a silicon-based "biochip" comprising nucleic acid immobilized thereon. In one embodiment, the silicon comprises microcavities. The nucleic acid to be assayed for the presence of one or more target nucleic acid sequences is immobilized on the silicon. A nucleic acid, such as an oligonucleotide probe, having a sequence substantially complementary to the target nucleic aci
...

Cancer Platinum Resistance Detection and Sensitization Method

The phosphorylation status of the BAD protein is a determinant of ovarian cancer cell responsiveness to platinum chemotherapy. Indirect manipulation of BAD phosphorylation status influences cisplatin sensitivity. BAD phosphorylation represents a biomarker that predicts platinum sensitivity and is a therapeutic target to increase platinum sensitivity. The methods employ phospho-specific antibody against a particular amino acid residue or site. Phospho-specific protein characterization methods inc
...

Automated Particulate Concentration System

An automated system for concentrating potentially harmful substances from various water types or other non-viscous liquids to facilitate detection of those substances is disclosed herein. The automated system comprises a water pressure driven or pump driven concentration unit that filters the test fluid through a hollow-fiber filter. Material collected on the filter is backflushed into a collection vessel by passing a small volume of sterile solution through the filter in the reverse direction.
...

Cannabinoid 2 (Cb2) Receptor Gene Promoter and Unique RNA Transcripts in B Cells and Methods of Use

Cannabinoid receptor 2 (CB2) is expressed in B lymphocytes and is involved in immune regulation. Mouse splenic B cells express three CB2 transcripts utilizing two different first exons. Human peripheral blood B cells express one CB2 transcript utilizing one first exon. Alignment of sequenced RACE products to either the mouse or human genome reveals that isolated transcripts contain previously unidentified transcriptional start sites (TSS). B cells from mouse and human preferentially express one
...

Oncotherapeutic Application of Inhibitors of High-Affinity Glucose Transporters

The subject invention concerns materials and methods for treating oncological disorders in a person or animal using any agent or compound that inhibits uptake of glucose into a cell. The subject invention also concerns methods for inducing apoptosis and inhibiting the proliferation or survival of a cell. In one embodiment, the methods comprise administering an effective amount of an agent or compound that inhibits the activity of one or more glucose transporter proteins, such as Glut-1. An antib
...

Biomarkers for Predicting Response to Immunosuppressive Therapy

The subject invention concerns methods and materials for assessing a patient's likelihood of responsiveness to an immunosuppressive therapy. The subject invention is contemplated for use with patients having an autoimmune disorder. In an exemplified embodiment, the methods of the invention are used for assessing and/or treating a patient with MDS. In one embodiment, a method of the invention comprises analyzing T cells of a patient for dysregulation of CD4+ and/or CD8+ T cell subsets, and determ
...

Microfluidic Device for Controlled Aggregation of Spider Silk

The present invention is directed to a device and method of controlling the phase separation of one or a mixture of two or more spider silk proteins, leading to the defined and controllable assembly of the said silk protein(s) to defined morphologies, such as spheres, nano fibrils, threads, etc.
...

Metal-enhanced Bioluminescence: an Approach for Monitoring Biological Bioluminescent Processes

The present invention relates to surface plasmon-coupled bioluminescence, wherein bioluminescent emission from a bioluminescent chemical reaction couples to surface plasmons in metalized particles thereby enhancing the signal. Importantly, these plasmonic emissions emitted from metallic particles generated without an external excitation source but instead from induced electronically excited states caused by the bioluminescent chemical reaction.
...

High Affinity Recombinant Sea Lamprey Antibodies Selected By a Yeast Surface Display Platform

The present invention relates to a Yeast Surface Display (YSD) vector for expression of VLR proteins by yeast, wherein the vector includes nucleotide sequences encoding segments of yeast flocculation proteins FloIp, such as the leader and C-terminal segments, a homologous recombinant cassette and a geneticin/kanamycin resistance gene. The vector can be used for expression of VLR that may be effective in diagnostic applications (e.g., protein chip, immunohistochemistry, flow cytometry), immunoaff
...

Engineering Broad-spectrum Disease Resistance Against Haustorium-forming Pathogens Using RPW8 As a Delivery Vehicle

The present invention relates to the increased expression of RPW8 proteins causing an increased level of the RPW8 proteins at extrahaustorial membrane with subsequent induction of pathogen inhibition reactions against haustorium forming pathogens.
...

Electroaddressing and In-film Bioprocessing Using Stimuli-responsive Hydrogel-forming Polymers

Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a
...

Ubiquilin Regulation of Presenilin Endoproteolysis, and Suppression of Polyglutamine-induced Toxicity in Cells

Use of ubiquilin is described, including utilization for reducing fragmentation of presenilin 1 and 2 and to modulate [gamma]-secretase components, Pen-2 and Nicastrin, as well as utilization for inducing increased levels of ubiquilin to reduce aggregation of polyglutamine expansion proteins known to cause cell toxicity and cell death in subjects suffering from neurodegenerative diseases, such as Huntington's and Alzheimer's diseases.
...

Proteins Containing Expanded Polyglutamine Tracts Induce Mitochondrial Fragmentation By Disrupting Mitochondrial Fusion

Expanded polyglutamine tracts are shown to be a cause of disturbance of mitochondrial function and therefore a cause of cell toxicity and cell death and a cause of various diseases or disorders related to such effects. Such diseases and disorders may include neurodegenerative diseases or disorders, such as Huntington's Disease, Spinocerebellar ataxias, spinal and bulbar muscular atrophy, and dentatorubral-pallidoluysian atrophy. Methods for controlling such cell death, cell toxicity and/or relat
...

Recombinant Double-Stranded RNA Phage, and Use of the Same

A recombinant double stranded RNA (dsRNA) phage expresses dsRNA-encoded genes in eukaryote cells. Recombinant dsRNA phage are useful for the expression of dsRNA expression cassettes encoding passenger genes, such as, but not restricted to, vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs in eukaryotic cells or tissues. Methods are provided to deliver recombinant dsRNA phage to eukaryotic cells and tissues, either by direct administration, formul
...

Targeted Delivery of Imaging Probes for in Vivo Cellular Imaging

The present invention relates to imaging probes comprising immunoliposomes that encapsulate self-quenching concentrations of stable free radicals or high-relaxivity contrast agents, such as nitroxides or trityl radicals, wherein the immunoliposomes comprise a targeting ligand, positioned on the surface of the immunoliposomes and having affinity for targeted tissue.
...

Improved Folding of Recombinant Proteins Via Co-expression of Archaeal Chaperones

The present invention relates to recombinant protein production, and more specifically, to methods for recovery of properly folder bioactive proteins by expressing chaperone genes from extremophilic Archaea, during recombinant protein synthesis in a host cell thereby significantly improving recovery of properly folded bioactive proteins.
...

Fibrous Assemblies for Antibody Presentation, and Multiplexed Antigenic Analysis Using Same

Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber ass
...

Biosynthetic Pathway and Genes Required for Tropodithietic Acid Biosynthesis in Silicibacter TM1040

Production and use of a sulfur-containing compound, tropodithietic acid (TDA), from the roseobacter Silicibacter sp. TM1040 is described. Specifically, a biosynthetic and regulatory pathway for TDA biosynthesis in roseobacters is described. The TDA produced from roseobacters, specifically Silicibacter sp. TM1040, is shown to have antibacterial activity, in particular against Vibrio anguillarium, Vibrio cholerae, Vibrio coralliilyticus, Vibrio shiloi, Halomonas spp., Mycobacterium marinum, Mycoba
...

Radiation-resistant Mutants of a Halophilic Archaeon and Uses Thereof

The present invention relates to variants of the model halophile Halobacterium sp. NRC- 1 that have been exposed to cycles of irradiation with high doses of 18-20 MeV electrons. These novel isolated halobacterium exhibit increased resistance to radiation and may be used for bioremediation of toxic and highly radioactive waste sites and/or for studying and modulating the mechanism involved in such resistance.
...

Conversion of Just-continuous Metallic Films to Large Particulate Substrates for Metal-enhanced Fluorescence

The present invention relates to a method of producing silver films having large nanoparticles caused by cracking during anaerobic annealing to provide surfaces that exhibit increased metal enhanced fluorescence. Preferably the annealing process is conducted on a silver film having a thickness from about 14 to 17 nm for about an hour at a temperature of approximately 190 DEG C to about 210 DEG C resulting in the conversion of the just-continuous films into large particulate films, not readily as
...

Voltage-gated Metal-enhanced Fluorescence, Chemiluminescence Or Bioluminescence Methods and Systems

In the present invention, it is demonstrated for the first time, the influence of electrical current on the ability of surface plasmons to amplify fluorescence signatures. An applied direct current across silver island films (SiFs) of low electrical resistance perturbs the fluorescence enhancement of close-proximity fluorophores. For a given applied current, surface plasmons in "just-continuous" low resistance films are sparsely available for fluorophore dipole coupling and hence the enhanced fl
...

Microwave Trigger Metal-enhanced Chemiluminescence (MT MEC) and Spatial and Temporal Control of Same

The present invention relates to a method of imaging structures and features using plasmonic emissions from metallic surfaces caused by chemiluminescence based chemical and biological reactions wherein imaging of the reactions is enhanced by the use of microwave energy and further enhanced by using metallic geometric structures for spatially and temporally controlling the biological and chemical reactions.
...

Plants with Reduced Expression of Phosphatase Type 2C Gene for Enhanced Pathogen Resistance

The present invention relates to a method for down regulating an Arabidopsis protein phosphatase type 2C gene, referred to as "defense-associated protein phosphatase type 2C one" (DAPP1) that functions as a negative regulator of a plant defense pathway by contacting the gene or gene mRNA with an interfering nucleotide sequence that interacts with the gene and reducing expression thereof. Plants including such interfering nucleotide sequence exhibit increased disease resistance to pathogen even i
...

Glycosylation Engineered Antibody Therapy

The instant invention is drawn to methods of generating a glycosylation- engineered antibody, and using the glycosylation-engineered antibody for treating a patient, particularly a cancer patient or a patient with an immune disease or disorder. The instant invention is also drawn to methods of generating a glycosylation-engineered antibody for use in the treatment of patients having a polymorphism that does not respond to conventional antibody therapy. The instant invention is also drawn to meth
...

A Novel Composition of Matter and Method for Stimulating the Growth of Beneficial Microorganisms

The invention describes a novel composition of matter obtained from the leaves of green plants, which is useful in promoting the growth of beneficial microorganisms. Specifically, that the invention describes a hydrolysate prepared from plant leaf biomass (leaf biomass hydrolysate or 'LBH') which dramatically stimulates the growth of beneficial microorganisms. Use of LBH as a fermentation substrate can also stimulate rapid production of organic acids and other organic compounds.; LBH can be used
...

Compositions and Methods Utilizing Fibrin Beta Chain Fragments of the Bbeta Chain of Fibrinogen

A composition including a peptide sequence of the formula ssX1-X2, the peptide sequence corresponding to an amino acid sequence of a fibrin beta chain fragment of a Bbeta chain of fibrinogen, wherein X1 represents an N-terminal end of the peptide sequence, and X2 represents a C-terminal end of the peptide sequence, wherein the peptide sequence includes additional amino acids between X1 and X2, wherein the peptide sequence may contain a non-naturally occurring amino acid residue, wherein the pept
...

ZAP-70 As Predictor and Modulator of Effector Function of T Cells

In this application is described a novel, multiparameter analysis of TCR-coupled signaling and function in resting and activated naive and memory CD4 T cells, revealing a biochemical basis for immunological recall. Results reveal a novel biochemical signature imparted to memory CD4 T cells enabling efficacious responses through increased ZAP-70 expression and reduced accumulation of downstream signaling events.
...

ERG-1 Peptides and Polynucleotides and their Use in the Treatment and Diagnosis of Disease

The present invention relates to peptide and polynucleotide fragments of ERG-1, and in particular, human ERG-1a (HERG-1a) and its isoforms, and their use in the treatment and diagnosis of disease, especially cardiac diseases, such as arrhythmias, and cancer.
...

Compositions and Methods of Use of EPB1, and ErbB3 Binding Protein

Inhibition of the proliferation of hormone refractory prostate cancer cells is achieved by administering EPB1, an ErbB3 binding protein, in combination with another anti-proliferation therapy such as administration of antiandrogens, other anticancer agents, radiation therapy, or surgery. Administration of EPB1 reverses the phenotype of hormone-resistant prostate cancer cells to hormone-sensitive prostate cancer cells.
...

Vaccines and Immunomodulatory Therapies for Tularemia

The present invention relates to attenuated strains of Francisella tularensis from which genes in the guanine nucleotide biosynthetic pathway have been deleted and to methods for making the mutants. The invention further relates to the use of the attenuated strains to make vaccines.
...

Microcin H47 Plasmid Selection System

The present invention relates generally to stabilized expression plasmid systems. The stabilized expression plasmid systems comprise an expression vector that includes a plasmid maintenance system (PMS) and, optionally, one or both of a polynucleotide encoding a selected antigen under control of a promoter, and a polynucleotide encoding a selectable marker under control of a promoter. The use of the mchI protein as a selectable marker is found in preferred embodiments of the invention.
...

Breast cancer resistance protein (BCRP) antibodies

The Breast Cancer Resistance Protein is described, as well as the cDNA encoding said protein. This protein has been found to confer resistance to cancer chemotherapeutic drugs.
...

Novel Antiproliferative Factor and Methods of Use

A novel antiproliferative factor comprising a glycopeptide is disclosed. In specific embodiments, the novel antiproliferative factor is associated with the bladder. Compositions, diagnostic kits and reagents, and methods of using the compounds for identifying and/or treating interstitial cystitis and cancer are disclosed.
...

Immunoglobulin Constant Region Fc Receptor Binding Agents.

IVIG replacement compounds are derived from recombinant and/or biochemical creation of immunologically active biomimetic(s). These replacement compounds are then screened in vitro to assess each replacements compound's efficiency at modulating immune function. Particular replacement compounds are selected for further in vivo validation and dosage/administration optimization. Finally, the replacement compounds are used to treat a wide range of diseases, including inflammatory and autoimmune disea
...

Rapid Expression Cloning of Human Monoclonal Antibodies from Memory B Cells

The present application provides methods for producing human monoclonal antibodies without using hybridoma technology, antibodies produced used the described methods, and methods for using the antibodies to treat or prevent disease conditions (e.g., infection by pathogens such as the Human Immunodeficiency Virus).
...

Factor VIII Compositions and Methods

The present invention provides methods of increasing the half-life and/or specific activity of factor VIII. More specifically, the invention provides methods of increasing the half-life and/or specific activity of factor VIII by substituting one or more amino acids in the A2 domain. It further provides methods for producing such factor VIII mutants. The invention also provides polynucleotides encoding the mutant factor VIII, and methods of treating hemophilia using the polypeptides and polynucle
...

Novel Human Androgen Receptor Alternative Splice Variants As Biomarkers and Therapeutic Targets

The present invention relates to novel androgen receptor splice variants (AR3, AR4, AR4b, AR5 and AR8) and variants and fragments thereof which have a role in the progression of androgen independent prostate cancer. The invention further relates to compositions and methods which can be used to identify and treat prostate cancer based on these novel androgen receptor splice variants, as well as methods for screening agents which modulate the activity and/or expression of the androgen receptor spl
...

Compositions and Methods for the Innate Immune Response Pathway

Autoimmune diseases are the consequence of complex interactions between a mosaic of host genetic factors and etiologic elements. Celiac disease (CD) is an autoimmune disease prevalent in 1% of the general population, but is unique on two accounts; a) the majority (90%) of individuals with CD have the HLA class II DQ2 allele, the others HLA-DQ8 and b) the etiologic agent is gluten proteins from wheat and related prolamins in barley and rye. The disease process is generally considered to be mediat
...

Methods for Treating Cancers and Diseases Associated with 4-1BB (CD137) Expression

The present invention relates to the role of 4-1BB (CD137) ligand and anti-4-1BB (CD137) antibody in the treatment of cancers and diseases associated with 4-1BB (CD137) expression. More particularly, the present invention relates to the use of (i) 4-1BB (CD137) ligand for inducing proliferation and activation and promoting survival of B lymphocytes and (2) anti-4-1BB (anti-CD137) antibody for inhibiting proliferation and activation and inducing death of B lymphocytes.
...

Engineered Type IV Pilin of Clostridium Difficile

The present invention relates to engineered Clostridium difficile type IV pilin (tfp) genes, type IV pilin proteins which can serve as a diagnostic marker for identification of patients infected with C. difficile, and vaccines comprising type IV pilin proteins, antigenic fragments and variants thereof for therapeutic interventions.
...

Co-signaling Methods for Treating Cancers

Provided herein is a method of treating a neoplastic disease in an individual in need of such treatment, comprising the steps of: expanding ?d T lymphocytes in vitro; priming Natural Kill cells in vitro; and administering said expanded ?d T lymphocytes and said primed Natural Kill cells to said individual, thereby resulting in a cytotoxic or cytolytic effect. Further provided is a method of treating a neoplastic disease in an individual in need of such treatment, comprising the steps of: adminis
...

MAGE-A3/HPV 16 Peptide Vaccines for Head and Neck Cancer

The present invention relates to Trojan antigens, and immunogenic compositions comprising the Trojan antigens. The present invention also relates to methods of generating an immune response in a subject using the Trojan antigens or immunogenic compositions. The present invention further relates to methods of treating squamous cell carcinoma of the head and neck (SCCHN) using the Trojan antigens and immunogenic compositions of the present invention.
...

Targeting Nad Biosynthesis in Bacterial Pathogens

The emergence of multidrug-resistant pathogens necessitates the search for new antibiotics acting on previously unexplored targets. Nicotinate mononucleotide adenylyltransferase of the NadD family, an essential enzyme of NAD biosynthesis in most bacteria, was selected as a target for structure-based inhibitor development. To this end, the inventors have identified small molecule compounds that inhibit bacterial target enzymes by interacting with a novel inhibitory binding site on the enzyme whil
...

Antigen binding domains from fish

A process for the production of an antigen specific antigen binding domain using a transformed host containing an expressible DNA sequence encoding the antigen specific antigen binding domain, wherein the antigen specific antigen binding domain is derived from a variable region of the immunoglobulin isotype NAR found in fish.
...

Response Gene to Complement 32 (RGC-32) in Disease

The invention relates to antibodies that bind to a fragment of the Response Gene to Complement-32 (RGC-32), as well as methods of using these antibodies. Particular methods of using the antibodies of the present invention include, but are not limited to, methods of detecting RGC-32 in a sample, methods of assaying cell proliferation, as well as methods of detecting and/or monitoring the progression of abnormal conditions in a subject, where the disease states are associated with the presence or
...

Novel Retinamide Retinoic Acid Metabolism Blocking Agents

Retinoic acid metabolism blocking agents (RAMBAs). The RAMBAs may be used for treatment of cancer, including breast and prostate cancers. Methods for preparing novel retinamide RAMBAs. The methods include reacting RAMBAs with terminal polar carboxylic acid group with a variety of amines in the presence of suitable coupling reagents. The retinamide RAMBAs are potent inhibitors of the growth of prostate and breast cancer cells and may be useful for the treatment of these diseases in humans.
...

Non-hemolytic ClyA for Excretion of Proteins

The disclosure below provides a protein export system utilizing non-hemolytic variants of HlyE family member proteins for efficiently producing recombinant protein from a host cell. In a preferred embodiment, the protein export system utilizes protein export machinery endogenous to the host bacterium into which the protein export system vector is introduced.
...

Fluorescent Proteins and Uses Thereof

The present invention provides novel fluorescent proteins. In particular aspects of the invention, a novel fluorescent protein of the invention has an increase in quantum yield. In other particular aspects of the invention, a novel fluorescent protein of the invention has altered spectral properties such that, for example, the protein can be utilized in laser-based applications and methodologies.
...

Androgen Receptor Inactivation Contributes to Antitumor Efficacy of CYP17 Inhibitors in Prostate Cancer

Provided are methods of inhibiting CYP17 in a mammal, such as a human, that include administering an effective amount of at least one CYP17 inhibitor, such as VN/124-1, VN/125-1, VN/85-1, VN/87-1 and/or VN/108-1 to the mammal. Also provided are methods of down regulating androgen receptor (AR) protein expression and methods of antagonizing AR in a mammal that include administering to the mammal an effective amount of at least one active ingredient selected from VN/124-1, VN/125-1, VN/85-1, VN/87
...

Methods and Systems for Purifying Transferring And/or Manipulating Nucleic Acids

The disclosure provides methods, systems, and devices for purifying, transferring or manipulating nucleic acids while maintaining the 2D spatial relationship of the nucleic acids as they were present in the original sample having 2D spatial information.
...

Enhanced Cellulase Expression in S. Degradans

The invention provides organisms and methods of using and making organisms with enhanced cellulase expression.
...

Peptides for Transport of Therapeutics and their Carriers in Mouse Models and Humans

A system for targeted delivery of agents (e.g., molecular probes, diagnostic agents, therapeutic agents, imaging agents, research or analytical compounds, enzymes, peptides, proteins, lipids, lipoproteins, sugars, hormones, vitamins, nucleic acids, viruses, bacteria, and/or cells) including use of a composition containing the agent and a targeting moiety, specific for a determinant at the target location. An exemplary composition of the system includes a targeting moiety of one of peptides ?3, 2
...

Process for the Preparation of Beta and Alpha Cryptoxanthin

The present invention relates to a process for converting lutein and/or lutein esters to (3R)-[beta]-cryptoxanthin and (3R,6'R)-[alpha]-cryptoxanthin, suitable for human consumption as dietary supplements, by employing safe and environmentally friendly reagents. (3R)-[beta]-Cryptoxanthin and (3R,6'R)-[alpha]-cryptoxanthin are two rare food carotenoids that are not commercially available and the former exhibits vitamin A activity. In the first synthetic step, commercially available lutein and/or
...

Engineered Proteases for Affinity Purification and Processing of Fusion Proteins

The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.
...

Radioactively Marked Cyclopropane Derivatives of DAT Affine Tropanes

The invention relates to radioactively marked cyclopropane derivatives of DAT affine tropanes suitable for the quantitative, non-invasive imaging of striatal and extrastiatal dopamine transporters (DAT) in humans and test animals by means of positron emission tomography (SPECT), autoradiography, or radio assay.
...

Apparatus, Method, and Gel System for Analytical and Preparative Electrophoresis

The present invention relates to an electrophoresis apparatus comprising a gel chamber for receiving electrophoresis medium, a removable gel system, which is arranged in the gel chamber, having a separation gel for the electrophoretic separation of biological molecules such as nucleic acids or proteins, electric contact elements for generating an electric field through the separation gel, optionally a lid for fastening on the gel chamber, characterized in that the separation gel is delimited at
...

Active Microfluidic Membranes

The present invention relates to a biofabricated Active Microfluidic Membrane (AMM) in a microfluidic network of a microfluidic device and a method for the in situ biofabrication of such a microfluidic network. More specifically, the invention relates to devices exhibiting (and methods of) positioning (i.e., erecting, modifying or removing a membrane matrix in situ in a microchannel of a microfluidic network of a microfluidic device. In one embodiment, the membrane comprises a single type of mat
...

Sparsely Cross-linked Nanogels: a Novel Polymer Structure for Microchannel DNA Sequencing

The present invention is generally directed to novel polymeric materials for use in the electrophoretic separation of nucleic acids. In particular, the novel polymer materials are sparsely crosslinked nanogels, dissolved in an aqueous buffer to form solutions with moderate to high viscosity. The present invention further provides methods for generating such novel polymers, and related methods of their use.
...

Peptides, Peptidomimetics and Small Molecule Inhibitors of the Epstein-Barr Virus Mediated Fusion and Entry Process

Disclosed are compositions and methods for modulating Epstein-Barr virus (EBV) infection in a patient. The compositions may include peptides or peptidomimetics that are related to gp42 and function to inhibit EBV-mediated membrane fusion.
...

Self Assembling Peptide Systems and Methods

The present invention provides a self-assembling peptide system which utilizes a bioactive sequence which enhances transfection efficiency. In particular, the present invention provides compositions and methods for transfecting aggregates of cells at a higher efficiency.
...

Synthetic Peptide and Peptide Conjugates and Related Tissue Coupling Methods Via Transglutaminase Enzyme

Peptides and conjugates thereof comprising one or more bioactive agents which can be coupled to a tissue via a transglutaminase and related methods.
...

Composition and Method for Self-assembly and Mineralization of Peptide-amphiphiles

The present invention is directed to a composition useful for making homogeneously mineralized self assembled peptide-amphiphile nanofibers and nanofiber gels. The composition is generally a solution comprised of a positively or negatively charged peptide-amphiphile and a like signed ion from the mineral. Mixing this solution with a second solution containing a dissolved counter-ion of the mineral and/or a second oppositely charged peptide amphiphile, results in the rapid self assembly of the pe
...

Citric Acid Polymers

The present invention provides polymers (e.g., elastomeric citric acid polymers) and methods of making and using these polymers (e.g., as a biologically active molecule delivery platform). In certain embodiments, the polymer has adsorbed biologically active molecules. In particular embodiments, the polymer comprises pores that are between about 7 and 15 nanometers in diameter. In other embodiments, the polymer comprises poly(1,8 octanediol-co-ctric acid). In certain embodiments, the polymers are
...

Methods and Compositions for Generating Sporulation Deficient Bacteria

The present invention relates to methods and compositions for engineering sporulating bacterial cells, particularly a cell of the class Clostridia. In particular, the present invention relates to the generation of sporulation deficient bacteria for the generation of industrial superior phenotypes.
...

Anti-cancer Compounds

The present invention relates to anti-cancer compounds, methods for their discovery, and their therapeutic use. In particular, the present invention provides analogs of the known anti-cancer compound amonafide, and structurally and functionally related compounds, and methods of using such compounds as therapeutic agents to treat a number of conditions associated with hyperproliferation.
...

Novel Biodegradable Elastomeric Scaffold for Tissue Engineering and Light Scattering Fingerprinting Methods for Testing the Same

The present invention is directed to a novel biocompatible polymer that may be used in tissue engineering. More specifically, the specification describes methods and compositions for making and using a citric acid copolymers.
...

Potent and Selective Neuronal Nitric Oxide Synthase Inhibitors with Improved Membrane Permeability

Compounds and related compositions and methods as can be used to inhibit neuronal nitric oxide synthase and as can be employed in the treatment of various neurodegenerative diseases.
...

Compositions and Methods for Induction of Antigen-specific Tolerance

The present invention utilizes carrier particles to present antigen peptides and proteins to the immune system in such a way as to induce antigen specific tolerance. The carrier particle is designed in order to trigger an immune tolerance effect. The invention is useful for treatment of immune related disorders such as autoimmune disease, transplant rejection and allergic reactions.
...

Method of Modulating HSF-1

The present invention is directed to methods of modulating HSF1 activity comprising modifying the acetylation of the DNA binding domain of the HSF1.
...

Cysteine Protease Autoprocessing of Fusion Proteins

Disclosed are fusion proteins, polynucleotides that encode the disclosed fusion proteins, and methods for expressing and autoprocessing of the disclosed fusion proteins to obtain a target protein. The disclosed fusion proteins include an autoproteolytic cysteine protease fused to a heterologous polypeptide, which may be isolated as the target protein. Preferably, the protease activity of the cysteine protease is inducible. Suitable autoproteolytic cysteine proteases for the fusion proteins inclu
...

Manipulation of Neuronal Ion Channels

The present invention provides compositions and methods for the manipulation of ion channels. For example, the present invention relates to Parkinson's and other neurological diseases and conditions, and treatments thereof. In particular, the present invention provides methods of decreasing pathophysiological high frequency neuronal bursts of Parkinson's and other neurological diseases and conditions. Methods of the present invention comprise decreasing Kv3 ion channel activity in fast-spiking n
...

Polyvalent RNA-nanoparticle Compositions

The present invention concerns nanoparticles functionalized with duplex RNA for a variety of uses, including but not limited to gene regulation. More specifically, the disclosure provides a new strategy for conjugating RNA to a nanoparticle to achieve increased stability and activity.
...

TARGET DNA INTERFERENCE WITH crRNA

The present invention provides methods, systems, and compositions for interfering with the function and/or presence of a target DNA sequence in a eukaryotic cell (e.g., located in vitro or in a subject) using crRNA and CRISPR-associated (cas) proteins or cas encoding nucleic acids. The present invention also relates to a method for interfering with horizontal gene transfer based on the use of clustered, regularly interspaced short palindromic repeat (CRISPR) sequences.
...

Methods and Compositions for Isolating Nucleic Acid

The present invention relates to compositions and methods for isolating and purifying nucleic acid. In particular, the present invention relates to methods of isolating nucleic acid from cells for use in further analysis.
...

Inhibition and Treatment of Prostate Cancer Metastasis

The present invention provides compounds and methods of inhibiting and treating metastatic prostate cancer. The compounds include MEK4 inhibitors. In another aspect the invention provides methods of identifying inhibitors of metastatic prostate cancer by screening for inhibitors of MEK4.
...

Lateral Flow Strip and Uses Thereof

The present invention relates to lateral flow strip assay system and uses thereof. In particular, the present invention relates to lateral flow assay systems for the simple and inexpensive detection of biomolecules.
...

Novel Peptide-based Scaffolds for Cartilage Regeneration and Methods for their Use

Disclosed herein are novel peptide amphiphile molecules and compositions composed of a peptide sequence that non-covalently binds the growth factor TGF-ss1. Also disclosed are methods of using these peptide amphiphiles to create a gel scaffold in situ that enhances articular cartilage regeneration when used in combination with microfracture. Significant improvement in tissue quality and overall O'Driscoll histological scores were observed in rabbits with full thickness articular cartilage defect
...

Multiplexed Biomolecule Arrays Made By Polymer Pen Lithography

Methods of patterning multiple biomolecules on a surface are disclosed. The method includes inking a polymer pen array, where tips are inked with selected inks comprising the biomolecules, and transferring the biomolecules to a surface using a polymer pen lithography technique. Methods of using the multiple patterned biomolecules on a surface are also disclosed.
...

Interpenetrating Biomaterial Matrices and Uses Thereof

The present invention relates to matrices (e.g., fibrin-alginate matrices; fibrin-alginate-matrigel matrices) for culture of cells, organs (e.g., ovary or fragment thereof), cells and cell aggregates (e.g., ovarian follicles, embryoid bodies), and tissues. In some embodiments, protease inhibitors e.g., aprotinin) are used to prevent the degradation of fibrin.
...

Biomarkers for Prostate Cancer

The present invention relates to compositions and methods for the detecting, treating, and empirically investigating the prostate. In particular, the present invention provides compositions and methods for using neuroligin biomarkers (e.g., NLGN-4Y) in the diagnosis, treatment, and empirical investigation of prostate disorders (e.g., prostate cancer, benign prostatic hypertrophy).
...

Methods for Nucleic Acid Transfer Into Cells

The present invention provides methods for increasing the transfer of nucleic acids into cells. In particular, the present invention provides for the use of inhibitors of HDAC6, a cytoplasmic histone deacetylase present in mammalian cells by, for example, small molecules or siRNA treatment, in increasing gene transfer and/or expression in cells in vitro and in vivo for research and gene therapy applications.
...

Non-enzymatic Detection of Bacterial Genomic DNA

The present invention relates to methods for detecting for the presence of one or more target analytes, e.g. biomolecules, in a sample. In particular, the present invention relates to a method that utilizes blocking strands to inhibit target rehybridization to detect double stranded genomic DNA.
...

Self-Assembly of Peptide-Amphiphile Nanofibers Under Physiological Conditions

Peptide amphiphile compounds, compositions and methods for self-assembly or nanofibrous network formation under neutral or physiological conditions.
...

Detection of Maternal Alcohol Exposure

The present invention provides methods, compositions, and systems for detecting in utero alcohol exposure by detecting expression level changes in certain biomarkers (e.g., in placental tissue). In certain embodiments, the biomarkers are selected from glucocorticoid receptor (GR), thyroid hormone receptor alpha (TR[alpha]), iodothyronine deiodinase III (Dio3) and G-protein [alpha]-subunit (Gs[alpha]).
...

Allelic Disorders Caused By Mutations in TRPV4

The present invention provides methods, kits, and compositions for detecting mutations in transient receptor potential cation channel, subfamily V, member 4 (TRPV4). In particular, mutations are detected in TRPV4 to detect diseases such as scapuloperoneal spinal muscular atrophy (SPSMA) and hereditary motor and sensory neuropathy type IIC (HMSN IIC) or Charcot-Marie-Tooth disease type 2C (CMT2C).
...

Surface-immobilized Antimicrobial Peptoids

Immobilizable antimicrobial compounds incorporating antimicrobial and/or antifouling components, as can be adhered to various device structures and components.
...

Self-assembling peptide amphiphiles and related methods for growth factor delivery

The invention provides peptides that are capable of binding growth factors, as well as peptide amphiphiles comprising certain of these peptides, micellar assemblies of such amphiphiles and related methods of use.
...

Label-free Biosensors Based Upon Distributed Feedback Laser

A biosensor based upon a vertically emitting, distributed feedback (DFB) laser is disclosed. In one configuration, the DFB laser comprises a replica-molded, one- or two- dimensional dielectric grating coated with a laser dye-doped polymer as the gain medium. A sensor is also described in which the grating layer and the active layer are combined into a single layer. DFB lasers using an inorganic or organic thin film with alternating regions of high and low index of refraction as the active layer
...

Altered Intracellular Localization of BRK/Sik Protein Tyrosine Kinase in Human Prostate Tumors

The invention provides methods for detecting abnormal prostate conditions, such as benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN), and adenocarcinoma, in an animal by comparing the amount of the Breast Tumor Kinase (BRK) tyrosine kinase in the nuclei of prostate luminal epithelial cells in a test sample with an amount of nuclear BRK protein in epithelial cells of normal prostate glands.
...

Anti-Antiogenic Fragments of Pigment Epithelium-Derived Factor (PEDF)

The present invention provides anti-angiogenic derived from pigment epithelium-derived factor (PEDF) pharmaceutical compositions comprising the peptides, and methods of preventing angiogenesis. Such methods are useful in treating angiogenesis-associated disorders and diseases.
...

Three-dimensional Microfabricated Bioreactors with Embedded Capillary Network

In an aspect, the present invention uses projection micro stereolithography to generate three-dimensional microvessel networks that are capable of supporting and fostering growth of a cell population. For example, provided is a method of making a microvascularized bioreactor via layer-by-layer polymerization of a photocurable liquid composition with repeated patterns of illumination, wherein each layer corresponds to a layer of the desired microvessel network. The plurality of layers are assembl
...

Neutralization of Staphylococcal and Streptococcal Toxins

Staphyloccocus and Streptococcus bacteria secrete various toxins that act as superantigens by stimulating a large fraction of the host's T cells. The binding of toxins to the variable domains of T cell receptor ss chains (Vss) leads to massive release of inflammatory molecules and, potentially, toxic shock syndrome (TSS). Soluble forms of different Vss domains able to bind superantigens with high-affinity have previously been generated. However, to neutralize unknown toxins or multiple toxins a
...

Peptides and Regulation of Calcium Channels

Calcium channels can be regulated by natural gamma proteins. Herein we disclose embodiments of compositions and methods, particularly involving short peptides which are capable of regulating calcium channel function. Certain short peptides which can inhibit calcium current have structural features from the first transmembrane domain of gamma6 such as a GxxxA motif and adjoining aliphatic residues. In embodiments the peptide compositions and methods are capable of selective efficacy for low volta
...

RHO1-Gamma Amino Butyric Acid C Receptor-Specific Antibodies

This invention provides antibodies immunologically specific for rho1-GABAC receptor protein. The invention also provides methods of making and methods of using said antibodies and kits containing the antibodies.
...

Production of Neural Stem Cells from Bone Marrow Tissue and Use Thereof

The invention provides reagents and methods for preparing mammalian mesenchymal-derived neural stem cells, especially autologous mesenchymal-derived neural stem cells, compositions thereof, and methods for using and administering the cells in a patient in need thereof.
...

Uses of Bispecific Antibody Coated Dendritic Cells Pulsed with Antigens and GM-CSF in Immune Regulation

GM-CSF administered before immunization exerted a sustained suppressive effect against the induction of myasthenia gravis (MG). This suppression was associated with lowered serum autoantibody levels, reduced T cell proliferative responses to AChR, and an expansion in the population of FoxP3+ regulatory T cells. Manipulating DCs to expand regulatory T cells is useful for the control of autoimmune diseases such as myasthenia gravis MG.
...

Method of Promoting Neurogenesis By Modulating Secretase Activities

This invention provides methods and reagents for promoting neurogenesis, by modulating neural stem cell proliferation and differentiation. Particularly, this invention provides methods and reagents for promoting neurogenesis in a patient's central nervous system where the patient suffers from an aging-related neurodegenerative disease. Specifically, the invention provides methods for promoting neurogenesis comprising modulating the a and/or ?-secretase activities.
...

Compositions and Methods to Prevent Cancer with Cupredoxins

The present invention discloses methods and materials for delivering a cargo compound into a cancer cell. Delivery of the cargo compound is accomplished by the use of protein transduction domains derived from cupredoxins. The cargo compound may be a nucleic acid and specifically a DNA, RNA or anti-sense. The invention further discloses methods for treating cancer and diagnosing cancer.
...

Hemicellulose-degrading Enzymes

The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from Prevotella bryantii. Hemicellulose-degrading enzymes include an endoxylanase, a ss-xylosidase, a bifunctional ss-xylosidase and ss-glucosidase, a bifunctional arabinofuranosidase and ss-xylosidase, a glucuronidase, and an acetyl xylan esterase. The enzymes can be used to release sugars present in hemicellulose for subsequent fermentation to produce value-added products
...

Design, Synthesis and Evaluation of Procaspase Activating Compounds As Personalized Anti-cancer Drugs

Compositions and methods are disclosed in embodiments relating to induction of cell death such as in cancer cells. Compounds and related methods for synthesis and use thereof, including the use of compounds in therapy for the treatment of cancer and selective induction of apoptosis in cells are disclosed. Compounds are disclosed that have lower neurotoxicity effects than other compounds.
...

Stimuli-responsive Low Solubility Hydrogel Copolymers of N-isopropyl Acrylamide and Synthesis Method

Preferred embodiment stimuli-responsive low solubility copolymer hydrogel compositions of the invention include polymerized N-isopropylacrylamide and water insoluble monomer or oligomer repeating units. The repeating units are arranged within the polymer backbone of the copolymer hydrogel. In a preferred fabrication method, precursors of N-isopropylacrylamide and precursors of the water insoluble monomer of oligomer are solved in a liquid solvent to form a solution in a container. An initiator i
...

Infertility Associated DEFB-126 Deletion Polymorphism

The present application provides diagnostic methods for determining the fertility status of a male individual by evaluating his DEFB-126 phenotypic and genotypic status. The present invention relates to a dinucleotide deletion polymorphism in the protein coding sequence of a DEFB-126 nucleic acid. The amino acid sequence of this variant has a significantly altered the carboxyl terminal, carbohydrate-containing domain of DEFB-126 in comparison to a wild-type DEFB-126 polypeptide. This variant res
...

Human Single-chain T Cell Receptors

A soluble human single-chain T cell receptor (TCR) having the structure: Va2-L-Vss or Vss-L-Va2, wherein L is a linker peptide that links Vss with Va, Vss is a TCR variable ss region, and Va2 is a TCR variable a region of the family 2 is provided. The provided scTCR is useful for many purposes, including the treatment of cancer, viral diseases and autoimmune diseases.
...

Use of polypeptides of cupredoxin family in cancertherapy.

Cytotoxic factors having use in modulating cell death, and their use in methods of treating necrosis or apoptosis-related conditions are disclosed. The invention also relates to methods for identifying active agents useful in treating conditions related to cell death or uncontrolled growth. The present inventors have found that different microorganisms produce different cylotoxic factor(s) having anticancer activity. The substantially pure cytotoxic factors can be used in a method of treating an
...

COMPOSITIONS AND METHODS FOR INHIBITING Ga PROTEIN SIGNALING

The present invention comprises compositions and methods for specifically inhibiting signaling through Ga13, Gai and Gaq subunits.
...

Activated Mesenchymal Stem Cells for the Prevention and Repair of Inflammatory States

Inflammatory cytokines e.g. IFN-? serve as initiating stimuli for MSC immunosuppresive activity in vivo. Other inflammatory cytokines, such as TNF alpha, the molecule hemoxygenase I, and TLR ligation of MSC may also provide such a response.
...

Regulation of Apoptosis By Neural Specific Splice Variants of IG20

Pro-apoptotic signaling caused by down-modulation of KIAA0358 or expression of IG20-SV4 effectively induces spontaneous apoptosis and sensitization to TNFa-induced apoptosis in neuroblastoma cells. Methods and composition to enhance cell death in neuroblastoma are provided. Methods and compositions to reduce cell death in neurodegenerative disorders are provided.
...

Fitness assay and associated methods

The present invention provides an assay for determining the biochemical fitness of a biochemical species in a mutant replicating biological entity relative to its predecessor. The present invention further provides a continuous fluorogenic assay for measuring the anti-HIV protease activity of protease inhibitor. The present invention also provides a method of administering a therapeutic compound that reduces the chances of the emergence of drug resistance in therapy. The present invention also p
...

Soybean Gene for Resistance to Aphis glycines

An Aphis glycines resistance Rag2 gene is provided herein, along with methods for identifying its presence using marker-assisted selection. A cultivar of G. max having resistance to Aphis glycines conferred by the Rag2 gene has been identified. The Rag2 gene, as well as the methods, aphid-resistant varieties, and markers disclosed herein may be used to breed new elite lines expressing soybean aphid resistance.
...

Pak1 Agonists and Methods of Use

The present invention is directed to Pak1 agonists and methods of use.
...

High affinity TCR proteins and methods

T cell receptors (TCRs) that have higher affinity for ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell r
...

Method for modulating or controlling sodium channel current by reactive oxygen species (ROS) originating from mitochondria

Method for modulating or controlling sodium channel current of a cell includes inducing mitochondrial reactive oxygen species (ROS) production in the cell.
...

Identification and Use of Peptide Inhibitors of Protein Synthesis

The present invention discloses compositions of peptide inhibitors of protein synthesis, and methods of identifying peptide inhibitors that are capable of inhibiting protein synthesis through an interaction at a stem-loop H18 in 16S rRNA of a 30S ribosomal subunit. Screening methods for peptides are disclosed, in addition to methods of determining the affinity of a test compound for a ribosomal subunit.
...

Hypervirulent Mutant of Agrobacterium Tumefaciens

This disclosure relates to a mutant Agrobacterium tumefaciens that is functionally deleted for the atu1060 gene that codes for the cyclic di-GMP synthase Atu1060, as well as methods for its use in transforming plants with desired transgenes. Such bacteria are more virulent than currently used strains of A. tumefaciens, and thus can be used to transform a wider variety of plants, such as plants that are traditionally recalcitrant to such transformation.
...

Use of Rpa4 and RPA4/RPA32 Hybrid Polypeptides for Modulating Cell Proliferation

Disclosed are polynucleotides and polypeptides that are useful for modulating cell proliferation. The polynucleotides may comprise coding regions for RP A4 and RPA4/RPA32 hybrid polypeptides. The polynucleotides may be used in gene vectors for inhibiting cell proliferation in a patient in need thereof.
...

IMMUNOGENIC COMPOSITIONS FOR ACTIVATING ?d T CELLS

Disclosed are compositions, kits, and methods for activating, expanding, or stimulating ?d T cells that include recombinant attenuated microbes. The compositions may include pharmaceutical compositions that are used as ?d T cell stimulating vaccines or immunogenic compositions for preventing or treating microbial infections and hyperplasias such as cancer.
...

Zinc Finger Nuclease for the CFTR Gene and Methods of Use Thereof

The present invention provides new zinc finger proteins and zinc finger nuclease (ZFNs) that find particular using in repairing the cystic fibrosis transmembrane conductance regulator (CFTR) gene.
...

Direct Oxidation of Calcium/Calmodulin Dependent Protein Kinase II and Associated Diagnostic and Therapeutic Methods

Calcium/calmodulin dependent protein kinase II (CaMKII) has been found to be directly oxidized, and direct oxidation of CaMKII was observed to result in calcium independent activation of CaMKII. Antibodies that bind specifically to oxidized forms of CaMKII (oxCaMKII) were generated and were utilized to detect oxCaMKII in blood from: (1) mice with cancer; (2) mice with a knock out of the gene encoding methionine sulfoxide reductase; (3) mice injected with angiotensin II; (4) mice injected with ba
...

Immunostimulatory Nucleic Acids

The invention relates to immunostimulatory nucleic acid compositions and methods of using the compositions. The T-rich nucleic acids contain poly T sequences and/or have greater than 25% T nucleotide residues. The TG nucleic acids have TG dinucleotides. The C-rich nucleic acids have at least one poly-C region and/ore greater than 50% c nucleotides. These immunostimulatory nucleic acids function in a similar manner to nucleic acids containing CpG motifs. The invention also encompasses preferred C
...

Variant TLR4 Nucleic Acid and Uses Thereof

The invention provides methods to identify polymorphisms at the human TLR4 locus, as well as methods to identify individuals at risk of indications that increase their morbidity and mortality.
...

Human Protein Acyl Transferases and Methods of Uses Therefor

The present invention provides the identification of human Ras palmitoyl acyl transfersase complexes, and nucleic acids coding therefor. In addition, methods of screening for modulators of human Ras palmitoyl acyl transfersase, including high throughput yeast screens, are also provided.
...

Schweinfurthins and Uses Thereof

Disclosed is a method for preventing or treating an undesirable condition in a subject carrying cells homozygous null for the neurofibromatosis type 1 gene or subjects that are haploinsufficient for the neurofibromatosis type 1 gene, the method comprising administering to a subject in need thereof an effective amount of a schweinfurthin or schweinfurthin analog or derivative, or a pharmaceutically acceptable salt, prodrug, hydrate, or solvate thereof. Also disclosed is a new schweinfurthin compo
...

Baculoviruses with Enhanced Virion Production and a Method for the Production of Baculoviruses

A method of co-expressing a portion of the VSV G protein gene or a truncated "stem" portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that inclu
...

Long Acting Atrial Natriuretic Peptide (la-anp) and Methods for Use Thereof

Materials and methods related to making and using natriuretic polypeptides having a mutation that results in an extended carboxy terminus.
...

Combination Motif Immune Stimulatory Oligonucleotides with Improved Activity

A class of immunostimulatory nucleic acids having at least two functionally and structurally defined domains is provided. This class of combination motif immunostimulatory nucleic acids activates an immune response and is useful for treating a variety of immune realted disorders such as cancer, infectious disease, and allergic disorders. The nucleic acids also stimulate activation of natural killer cells and production of type 1 interferon.
...

Immunostimulatory nucleic acid molecules

Nucleic acid sequences containing unmethylated CpG dinucleotides that modulate an immune response including stimulating a Th1 pattern of immune activation, cytokine production, NK lytic activity, and B cell proliferation are disclosed. The sequences are also useful a synthetic adjuvant.
...

Rna Interference Suppresion of Neurodegenerative Diseases and Methods of Use Thereof

The present invention is directed to small interfering RNA molecules (siRNA) targeted against nucleic acid sequence that encodes huntingtin or ataxin-1, and methods of using these siRNA molecules.
...

Immunomodulatory oligonucleotides

Oligonucleotides containing unthylated CpG dinucleotides and therapeutic utilities based on their ability to stimulate an immune response in a subject are disclosed. Also disclosed are therapies for treating diseases associated with immune system activation that are initiated by unthylated CpG dinucleotides in a subject comprising administering to the subject oligonucleotides that do not contain unmethylated CpG sequences (i.e. methylated CpG sequences or no CpG sequence) to outcompete unmethyla
...

Schweinfurthin Analogues

The invention provides fluorescent schweinfurthin analogs of formula(I) which are useful as probes and for the treatment of cancer and other diseases.
...

New fusion peptide comprising a coupling of a silencing domain with small molecule peptide aptamer, is androgen receptor inhibitor, useful to treat prostate cancer and benign prostatic hyperplasia

Fusion peptide (I) comprising a coupling of a silencing domain with small molecule peptide aptamer, for the inhibition of androgen receptor, is new. - ACTIVITY : Cytostatic. - MECHANISM OF ACTION : Androgen receptor inhibitor.
...

Kidney Derived Stem Cells and Methods for their Isolation, Differentiation and Use

The invention relates generally to methods for isolation and culture of kidney stem cells, cells isolated by the methods, and therapeutic uses for those cells.
...

Multipotent Adult Stem Cells and Methods for Isolation

The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells.
...

Analysis of Brain Patterns Using Temporal Measures

A set of brain data representing a time series of neurophysiologic activity acquired by spatially distributed sensors arranged to detect neural signaling of a brain (such as by the use of magnetoencephalography) is obtained. The set of brain data is processed to obtain a dynamic brain model based on a set of statistically-independent temporal measures, such as partial cross correlations, among groupings of different time series within the set of brain data. The dynamic brain model represents int
...

DNA Aptamers

The present invention relates to rapid diagnosis of Salmonella contamination using DNA aptamers.
...

High Throughput Bioreactor

A continuous flow bioreactor system that includes a bioreactor, an optional post-bioreactor preparation chamber, a cell sorter, and an optional pre-bioreactor preparation chamber in a closed loop, useful for enriching a heterogeneous cell population growing in the bioreactor with an isolated subpopulation of cells.
...

Selective Inhibitors of c-Jun N-terminal Kinases

Compositions and methods for treating, preventing, or ameliorating one or more symptoms, disorders, or conditions associated with particular c-Jun N-terminal kinase(s) (JNKs) activity are provided. Compositions contain small molecules such as pyrazoloanthrones.
...

Anti-flavivirus Therapeutic

The present invention relates to anti-flavivirus compounds, including lycorine and derivatives thereof, and their use in treating a subject infected by a flavivirus. The present invention also relates to the use of the anti-flavivirus compounds for the prophylaxis of flavivirus infection. The present invention further relates to a method of suppressing viral RNA synthesis of a flavivirus. Also described is a method of preparing an anti-flavivirus compound for use in the treatment or prophylaxis
...

Homologous Recombination in Multipotent Adult Progenitor Cells

The invention relates to methods of altering gene expression by homologous recombination in a multipotent adult progenitor cell (MAPC). In particular, methods of producing a recombinant MAPC, of correcting a genetic defect in a mammal, of providing a functional and/or therapeutic protein to a mammal, and of transforming a MAPC are provided. MAPCs containing an erogenous DNA as well as recombinant MAPCs and their differentiated progeny are also provided.
...

Nucleotide Repeat Expansion-associated Polypeptides and Uses Thereof

Isolated polypeptides that are endogenously expressed from nucleotide repeat expansions are disclosed. In some cases, the polypeptides include polypeptide repeats. In some cases, the polypeptide repeats include at least five contiguous repeats of a single amino acid. In other cases, the repeats include at least six contiguous amino acids of a tetra- or penta-amino acid repeat block.
...

Neurotrophins and Uses Thereof

A neurotrophin polypeptide, and a biologically active fragment thereof, are described. Also, methods are disclosed that include providing a composition comprising the neurotrophin polypeptide to a subject, wherein the composition is effective to ameliorate at least one symptom or clinical sign of a condition treatable with a neurotrophin when the composition is administered to a subject in need of treatment for a condition treatable with a neurotrophin.
...

TAL Effector-mediated DNA Modification

Materials and methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; "TALENS") are provided.
...

Reducing Inflammation Using Cell Therapy

The invention provides methods for treating pathological conditions associated with an undesirable inflammatory component, including graft- versus -host disease. The invention is generally directed to reducing inflammation by administering cells that express and/or secrete prostaglandin E2 (PGE2). The invention is also directed to drug discovery methods to screen for agents that modulate the ability of the cells to express and/or secrete PGE2, such as PGE2 receptor agonists. The invention is als
...

Haemophilus Parasuis Polypeptides and Methods of Use

The present invention provides isolated polypeptides having oligopeptide permease activity and an amino acid sequence that has at least 80 % identity with a Haemophilus parasuis OppA polypeptide. Also provided by the present invention are isolated polynucleotides that encode the polypeptides described herein, and antibody that specifically binds a polypeptide described herein. The present invention further provides genetically modified microbes, such as attenuated Haemophilus parasuis strains an
...

Decellularization and Recellularization Apparatuses and Systems Containing the Same

The invention described herein provides systems and apparatuses for an initialpreparation of an organ or tissue scaffold comprising an extracellular matrix, and subsequent recellularization of the scaffold to ultimately form a resultant artificial organ or tissue incorporating the natural and original extracellular matrix. The techniques and equipment of the invention collectively minimize scaffold collapse, compression or physical damage to the organ as well as afford the advantages of signific
...

Treatment of Diseases Caused By Bacterial Exotoxins

Provided are high affinity T cell receptor variable regions that are useful for treating diseases caused by superantigens including atopic dermatitis, pneumonia and delayed wound healing. The variable regions contain mutants that result in high affinity binding to the superantigen.
...

Detection of nucleic and acid sequence differences using the ligase detection reaction with addressable arrays

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a seco
...

Mapc Generation of Lung Tissue

The present invention relates to methods of improving function in lung tissue by administering a population of multipotent adult progenitor cells ("MAPCs") or differentiated progeny thereof.
...

Modified Vitamin K-dependent Polypeptides

The invention provides vitamin K-dependent polypeptides with enhanced membrane binding affinity. These polypeptides can be used to modulate clot formation in mammals. Methods of modulating clot formation in mammals are also described.
...

Stem Cell Aggregates and Methods for Making and Using

The invention is directed to compositions of cell aggregates and methods for making and using the cell aggregates where the aggregates comprise cells that are not embryonic stern cells but can differentiate into cell types of at least two of ectodermal, undo dermal, and mesodermal embryonic germ layers, e.g., stem cells.
...

Modified Vitamin K-dependent Polypeptides

The invention provides vitamin K-dependent polypeptides with enhanced membrane binding affinity. These polypeptides can be used to modulate clot formation in mammals. Methods of modulating clot formation in mammals are also described.
...

Molecular Markers for Identification of Fat and Lean Phenotypes in Chickens

The invention provides molecular methods of screening chickens to determine those more likely to have a lean or fat phenotype by identifying the presence of at least one polymorphism in genetic material of a chicken in the thyroid hormone repressible gene (THRG) or its 3' untranslated region (SEQ ID NO: 1) that is associated with a fat phenotype or a lean phenotype. The invention also provides methods of screening chickens to identify a polymorphism associated with a fat or lean phenotype. The i
...

Novel Hydrogels and Uses Thereof

The present invention provides novel hydrogels and methods of making and using such hydrogels. The present invention provides hydrogels that may be formed by the self-assembly of peptides in solution. Such self-assembly may be brought about by a change in one or more characteristics of the solution. Characteristics of the solution that may be changed include pH, ionic strength, temperature, and concentration of one or more specific ions. In addition, hydrogels of the invention may be disassemble
...

Injectable Beta-hairpin Peptide Hydrogel That Kills Methicillin-resistant Staphylococcus Aureus

A peptide comprising the sequence VKVKVRVKVDPPTKVKVRVKV-NH2 forms a hydrogel which has the ability to shear-thin and recover. The hydrogel, both before and after shear-thinning, is capable of killing bacteria, including methicillin-resistant Staphylococcus aureus (MRSA).
...

Identification of Fat and Lean Phenotypes in Chickens Using Molecular Markers

The present invention provides methods of screening chickens to determine those more likely to have a lean or fat phenotype. The invention also provides methods of screening chickens to identify a polymorphism associated with a fat or lean phenotype.
...

Delivery of Hydrogels As Sprays

A method for delivering beta-sheet peptide hydrogels to a target surface by shear-thinning and spraying the peptide hydrogel on the surface is provided. The beta-sheet peptide hydrogels can be applied over a range of thicknesses and can cover broad surface areas. The beta-sheet peptide hydrogels may also include a therapeutic agent.
...

Fertility Enhancement Using Lipid Carriers and Bioactive Molecules

The invention relates to a composition and method for enhancing fertilization. Fertilization enhancement is achieved by effectively delivering bioactive molecules with a lipid anchor (GPI-linked proteins) to the surface of epididymal or ejaculated sperm. The process may be facilitated or promoted in the presence of Clusterin/APOJ, a well-known lipid carrier. The rate of delivery, or removal, of bioactive molecules is regulated by the concentration of lipid carrier present. The acquisition of the
...

Perlecan Fragments as Biomarkers of Bone Stromal Lysis

A method for the detection in a body fluid of perlecan polypeptide fragments that are biomarkers of tumor metastasis, and antibodies for detecting these fragments are described. An immunoassay kit for detecting the presence of these biomarkers in a body fluid, such as serum or urine, is also described.
...

Macromolecular Diffusion and Release from Self-assembled B-hairpin Peptide Hydrogels

A hydrogel for delayed release of an anionic macromolecule, wherein the hydrogel comprises the anionic macromolecule, 150 mM NaCl, and a peptide selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:33 in an aqueous medium at a pH of 7.4; wherein the anionic macromolecule has an isoelectric point of at most 6.8; and wherein the hydrogel is capable of retaining at least 25% of the anionic macromolecule after 28-day extraction at 37 DEG C with a pH=7.4 BTP buffer containing 150 mM Na
...

Methods and Compositions for the Display of Polypeptides on the Pili of Gram-positive Bacteria

Provided herein are methods and compositions for the display of polypeptides of interest on the tip of pili of Gram-positive bacteria. According to the present invention, the polypeptide of interest is amino terminal to a Gram-positive bacterial pilus tip protein or an active variant or fragment thereof, wherein the active variant or fragment comprises a cleaved cell wall sorting signal (CWSS) motif. The Gram-positive bacterium displaying a polypeptide of interest on the tip of pili that are dis
...

Coated Quantum Dots and Methods of Making and Using Thereof

The present disclosure provides embodiments of a new class of hydroxylated quantum dots. The quantum dots have a hydroxylated coat disposed thereon, and which serves to minimize non-specific cellular binding and to maintain the small size of quantum dot probes. Embodiments of the coated quantum dots of the disclosure are just slightly larger than the diameter of uncoated quantum dots, and are bright with high quantum yields. They are also very stable under both basic and acidic conditions. Embod
...

Immunogenic Compositions and Methods of Use Thereof

Embodiments of the disclosure encompass compositions and methods for generating immune responses in an animal or human host Embodiments of the compositions encompass proteins derived from the surface proteins of bacteria and protozoa, and in particular the flagellum component flagellin, and which have adjunctival properties when administered in conjunction with an immunogen Embodiments of the compositions of the disclosure are modified to incorporate a heterologous transmembrane-cytoplasmic doma
...

Compositions and methods for generating an immune response

We have developed DNA and viral vectors that can be used, alone or in combination, as a vaccine against one HIV clade, subtype, or recombinant form of HIV or against multiple HIV clades, subtypes, or recombinant forms. Moreover, the vectors can encode a variety of antigens, which may be obtained from one clade or from two or more different clades, and the antigens selected and/or the manner in which the vectors are formulated (e.g., mixed) can be manipulated to generate a protective immune respo
...

Modulation of Sodium Channels By Nicotinamide Adenine Dinucleotide

The present invention relates to the use of oxidized nicotinamide adenine dinucleotide (NAD+) or of its reduced form, NADH, as sodium channel modulators. The present invention also relates to the use of compositions containing NAD+ or NADH to treat conditions associated with sodium channel current, such as arrhythmia. NAD+ is found to increase sodium channel current, while NADH is found to decrease sodium channel current. Thus, conditions that are associated with decreased sodium channel current
...

Methods of Making Monoclonal Antibodies Using Fusion-Peptide Epitope Adoptive Transfer (F-PEAT) Technology

Disclosed are compositions and methods for related to monoclonal antibodies specific for single amino acid variation in an antigen.
...

Methods for Determination of Protein Phosphatase Activity, and Uses in Predicting Therapeutic Outcomes

One aspect of the present disclosure encompasses methods for determining a protein kinase or phosphatase activity in a biological sample, comprising: contacting in a reaction mix a first test sample and a fluorescently-labeled peptide substrate capable of being modified by a protein phosphatase or a protein kinase, contacting the reaction mix with a TiO2 matrix, thereby partitioning fluorescently-labeled phosphorylated peptide from fluorescently-labeled dephosphorylated peptide; and determining
...

BHC80 - Histone Complexes and Uses Thereof

Provided herein is the tertiary structure of domains of the BHC80 protein, including the PHD finger domain, such as when the BHC80 protein is complexed with an unmodified histone H3 tail in the presence of zinc. The invention provides structural and functional information for use in the identification and design of compounds that agonize, antagonize or otherwise regulate enzyme (e.g., demethylase) activity, and to the compounds identified by such methods and the research, diagnostic and therapeu
...

Re-engineered UV Damage Endonuclease, Compositions and Methods

Provided are methods and compositions for reducing damage to skin by ultraviolet light and other agents that cause distortion to double stranded DNA and methods for reducing damage to other organs due to such DNA distortion. These compositions comprise a novel truncated UV damage endonuclease (a truncated derivative of Uvde 1 (UVDE, Uvelp) of Schizosaccharomyces pombe) in conjunction with a cell penetrating peptide, together with components suitable for topical application or other administratio
...

Heterocyclic Peptide Ketoamides

A novel class of peptide a-ketoamides useful for selectively inhibiting calpains, selectively inhibiting cysteine proteases, and generally inhibiting all cysteine proteases, having the formula M-AA2-AA1-CO-NH-(CH2)n-R3. Processes for the synthesis of peptidyl a-ketoamide derivatives. Compositions and methods for inhibiting cysteine proteases, inhibiting calpains, and treating disease caused by cysteine proteases and calpains are provided.
...

PH-dependent NMDA receptor antagonists

NMDA receptor blockers, including pH-sensitive NMDA receptor blockers, are provided as neurprotective drugs that are useful in stroke, traumatic brain injury, epilepsy, and other neurologic events that involve acidification of brain or spinal cord tissue. Compositions and methods of this invention are used for treating neurodegeneration resulting from NMDA receptor activation. The compounds described herein have enhanced activity in brain tissue having lower than normal pH due to pathological co
...

Imaging Agents

This invention provides amino acid derivatives useful in detecting and evaluating brain and body tumors, including (1S,2S) anti-2-[18F]FACPC and (1R,2R) anti-2-[18F]FACPC.
...

Alzheimer's Disease Imaging Agents

This invention provides compounds and methods of imaging amyloid deposits using radiolabeled compounds. This invention also provides a method of inhibiting the aggregation of amyloid proteins to form amyloid plaques or deposits, a method of determining a therapeutic compound's ability to inhibit aggregation of amyloid protein, and a method of delivering a therapeutic agent to amyloid deposits.
...

Use of Rankl to Induce Differentiation of Microfold Cells (M Cells)

The differentiation of microfold (M) cells is important in mucosal immunity. The differentiation of M cells is desirable, such as in situations in which a host response to an antigen is sub-optimal, or wherein increased uptake of a compound is desired. Methods are provided herein for increasing a mucosal immune response. The methods include selecting a subject in need of an increased mucosal immune response and administering to a subject a therapeutically effective amount of RANKL or another ago
...

Purine Nucleoside Monophosphate Prodrugs for Treatment of Cancer and Viral Infections

The present invention is directed to compounds, compositions and methods for treating or preventing cancer and viral infections, in particular, HIV, HCV, Norovirus, Saporo virus, HSV-I, HSV-2, Dengue virus, Yellow fever, and HBV in human patients or other animal hosts. The compounds are certain 6-substituted purine monophosphates, and pharmaceutically acceptable, salts, prodrugs, and other derivatives thereof. In particular, the compounds show potent antiviral activity against HIV-I, HIV-2, HCV,
...

9-aminonoscapine and Its Use in Treating Cancers, Including Drug- Resistant Cancers

9-aminonoscapine, prodrugs thereof, and pharmaceutically acceptable salts thereof, are disclosed. Pharmaceutical compositions including 9-aminonoscapine, and methods of preparation and use thereof are disclosed. 9-aminonoscapine is a noscapine analog that can be used to treat and/or prevent a wide variety of cancers, including drug resistant cancers, by binding tubulin and inducing apoptosis selectively in tumor cells (ovarian and T-cell lymphoma) resistant to paclitaxel, vinblastine and tenipos
...

Aminoquinoline Derived Heat Shock Protein 90 Inhibitors, Methods of Preparing Same, and Methods for their Use

Novel classes of molecular chaperone Heat shock protein 90 (Hsp90) inhibitors and methods for making these classes are provided herein. These compounds are useful in treating and preventing cancer and other Hsp90-related diseases, such as inflammation and neurodegenerative disorders. Also provided herein are methods of treating and preventing cancer and other Hsp90 related disease. The methods include administering to a subject a therapeutically effective amount of an Hsp90 inhibitor.
...

Heat Shock Protein 90 Inhibitors, Methods of Preparing Same, and Methods for their Use

Novel classes of molecular chaperone Heat shock protein 90 (Hsp90) inhibitors are disclosed. These compounds are useful in treating and preventing cancer and other Hsp90-related diseases and conditions, such as inflammation and neurodegenerative disorders. Methods of treating and preventing cancer and other Hsp90 related diseases and conditions are disclosed that include administering to the subject a therapeutically effective amount of an Hsp90 inhibitor. Methods of preparing the novel Hsp90 in
...

Prostate Cancer Biomarkers to Predict Recurrence and Metastatic Potential

Described herein are methods for predicting the recurrence, progression, and metastatic potential of a prostate cancer in a subject. For example, the method comprises detecting in a sample from a subject one or more biomarkers selected from the group consisting of FOXO1A, SOX9, CLNS1A, PTGDS, XPO1, LETMD1, RAD23B, ABCC3, APC, CHES1, EDNRA, FRZB, HSPG2, and TMPRSS2_ETV1 FUSION. The method can further comprise detecting in a sample from a subject one or more biomarkers selected from the group cons
...

Triarylmethane Analogs and their Use in Treating Cancers

Compounds, pharmaceutical compositions including the compounds, and methods of preparation and use thereof are disclosed. The compounds are triphenyl methane analogs. The compounds and compositions can be used to treat and/or prevent a wide variety of cancers, including drug resistant cancers, inflammatory, degenerative and vascular diseases, including various ocular diseases, and parasitic infections. Representative triphenyl methane analogs include triphenyl methane analogues of various dyes,
...

Inhibitors of Nox Enzymes and Methods of Use Thereof

Inhibitors of Nox enzymes, such as Noxl, Nox2, and Nox3, and methods of making and using such compounds are describe herein.
...

Use of mTOR Inhibitors to Enhance T Cell Immune Responses

It is disclosed herein that treatment of a subject with an mTOR inhibitor enhances antigen-specific T cell immune responses. Thus, provided herein is a method of enhancing an antigen-specific T cell response in a subject by administering to the subject a therapeutically effective amount of an mTOR inhibitor. The antigen can be any antigen, such as an antigen from a pathogen or a vaccine, or a tumor antigen. In some embodiments, the method further comprises administering to the subject a vaccine,
...

Human Anti-PD-1, PD-l1, and PD-l2 Antibodies and Uses Therefor

The present invention is based, in part, on the identification of novel human anti-PD-1, PD-L1, and PD-L2 antibodies. Accordingly, the invention relates to compositions and methods for diagnosing, prognosing, and treating conditions that would benefit from modulating PD-1, PD-L1, and/or PD-L2 activity (e.g., persistent infectious diseases, autoimmune diseases, asthma, transplant rejection, inflammatory disorders and tumors) using the novel human anti-PD-1, PD-L1, and PD-L2 antibodies described h
...

Drug Resistant Immunotherapy for Treatment of a Cancer

The present disclosure is generally related to methods for combining chemotherapy and immunotherapy for the treatment of a cancer. The methods also relate to generating a drug-resistant cytotoxic immune cell line and uses thereof in conjunction with cytotoxic drugs.
...

Tumor Imaging Compounds

The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of alpha-aminoisobutyric acid (AIB) analogs namely, their rapid uptake and prolonged retention in tumors with the properties of halogen sustituents, including certain useful halogen isotopes such as fluorine-18, iodine-123, iodine-124, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77, bromine-82, astatine-210, astatine-211, and
...

Eliciting Immune Responses Using Recombinant MVA Viruses Expressing HIV Env, Gag and Pol Anitgens

Methods for eliciting beneficial immune responses against HIV by administering to a subject a recombinant MVA virus expressing HIV env, gag, and pol antigens are described. The recombinant MVA is administered at least three times and, in certain embodiments, is administered to a patient that has not been treated with a DNA vaccine directed against HIV (i.e., has not been treated with a nucleic acid molecule encoding one or more HIV antigens). The methods can elicit production IgA antibodies dire
...

Gambogic Amine, a Selective TrkA Agonist with Neuroprotective Activity

Small molecule agonists, partial agonists, and antagonists for the TrkA receptor are described. The compounds are gambogic amines, where the carboxylic acid group of gambogic acid (CO2H) has been replaced by an amine group (CH2NR1R2). In some embodiments, the compounds selectively bind to TrkA but not TrkB or C, robustly induce its tyrosine phosphorylation and downstream signaling activation including Akt and MAP kinases. Further, they can strongly prevent glutamate-induced neuronal cell death a
...

Assessing Tumor Response to Therapy

Systems, methods and computer program products identify first biologic data in a region of interest in a first image and calculate a first biologic volume histogram from the first biologic data. Second biologic data in the same region of interest is identified in a second image and a second biologic volume histogram is calculated from the second biologic data. A difference in intensity for the region of interest is determined using the first biologic volume histogram and the second biologic volu
...

DWF4 Polynucleotides, Polypeptides and Uses Thereof

The present invention relates to novel, polynucleotides isolated from dwarf plants. The dwf4 polynucleotides that encode all, or a portion of, a DWF4 polypeptide, a cytochrome P450 enzyme that mediates multiple steps in synthesis of brassinosteriods. The present invention also relates to isolated polynucleotides that encode regulatory regions of dwf4. Uses of the dwf4 polypeptides and polynucleotides are also disclosed.
...

Immobilizing an Entity in a Desired Orientation on a Support Material

The present invention relates to the identification and selection of attachment molecules that attach/immobilize an entity having a detectable activity or property on a support in an orientation that provides a detectable activity or property, and to surfaces made of the attachment molecules.
...

Recombinant Bacterium Capable of Eliciting an Immune Response Against Streptococcus Pneumoniae

The invention encompasses a recombinant bacterium capable of eliciting an immune response against Streptococcus pneumoniae, a vaccine comprising the bacterium, and methods of using the bacterium.
...

Synthetic Antibodies

The present invention provides methods for synthetic antibodies, methods for making synthetic antibodies, methods for identifying ligands, and related methods and reagents.
...

Artificial Lectin Mimics and Uses Thereof

Lectins are important class of sugar-binding proteins involved in a variety of biological phenomena. In some aspects, the present invention relates to the process of selection and application of high-fidelity artificial lectin mimics in the construction of microarray devices capable of precise, rapid and inexpensive analysis of glycoproteins, pathogen detection, and diagnosis of various disease states. In some aspects, the invention also relates to the use of identified binding peptides on solid
...

Prostate Cancer Markers and Uses Thereof

The present invention provides methods for assessing the probability of a prostate tumor in a subject, or for monitoring efficacy of treatment for prostate cancer, involving detection of QSOX1 in patient tissue samples.
...

Pancreatic Cancer Markers and Uses Thereof

The present invention provides methods and reagents for assessing the probability of a subject having a pancreatic tumor, and for monitoring efficacy of treatments for pancreatic tumors.
...

Synthetic Antibodies

Methods for synthetic antibodies, methods for making synthetic antibodies, methods for identifying ligands, and related methods and reagents.
...

Synbodies to Akt1

The present application provides synbodies against AKT1 differing in amino acid sequence, conjugation chemistry, linker/scaffold, or adjunct moiety. The synbodies are useful for diagnosis and treatment of cancer and as research reagents.
...

Methods of Assessing a Risk of Cancer Progression

Methods and kits that use miRNA expression to predict the development of brain metastases in non-small cell lung cancer patients are disclosed.
...

Recombinant Edwardsiella Bacterium

The present invention encompasses a recombinant Edwardsiella bacterium, and compositions and methods of use thereof.
...

A Dna Replicon System for High-level Rapid Production of Vaccines and Monoclonal Antibody Therapeutics in Plants

Plant viral vectors have great potential in rapid production of proteins, but no simple Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric s
...

Molecular Films for Hydrophobic Implant Surfaces

Compositions are disclosed containing a solvated viscoelastic polymeric gel diluted into an ionically conductive aqueous solution which can be usefully applied to any surface that is hydrophobic to act, for example, as an antifogging coating with minimal optical distortion and excellent transparency. The compositions can also be used as lubricious agents on medical implants, shunts, and surgical supplies to minimize tissue trauma, to maximize bio-compatibility, and to increase healing by enhanci
...

Methods for Identifying Cancer Risk

Provided herein are tissue-specific differential methylated regions (T-DMRs) and cancer-related differential methylated regions (C-DMRs) and methods of use thereof. In one embodiment of the invention, there are provided methods of detecting a cell proliferative disorder by detecting altered methylation in one or more DMRs identified herein. In another embodiment of the invention, there are provided methods of determining clinical outcome by detecting altered methylation in one or more DMRs ident
...

Methods of treating measles infectious disease in mammals

The invention provides for a measles vaccine utilizing a human codon-optimized polynucleotide encoding a measles virus polypeptide, such as HA or F protein. Optionally, the vaccine is administered with an adjuvant and is especially useful for immunizing an infant mammal.
...

Cardiac Stem Cells

Human cardiac stem cells can be isolated from endomyocardial biopsies. Such cells mediate cardiac regeneration and improve heart function in a mouse infarct model. The cells can be used for autologous, allogeneic, syngeneic, or xenogeneic therapeutic applications in patients. The stem cells can be genetically modified to enhance their therapeutic activity.
...

Integrated Analyses of Breast and Colorectal Cancers

Genome-wide analysis of copy number changes in breast and colorectal tumors used approaches that can reliably detect homozygous deletions and amplifications. The number of genes altered by major copy number changes-deletion of all copies or amplification of at least twelve copies per cell-averaged thirteen per tumor. These data were integrated with previous mutation analyses of the Reference Sequence genes in these same tumor types to identify genes and cellular pathways affected by both copy nu
...

Bioluminescence Imaging-based Screening Assay and Inhibitors of Abcg2

A bioluminescence imaging-based high-throughput assay for inhibitors of ABCG2 is described. Compositions of inhibitors of ABCG2 and methods of using ABCG2 inhibitors are also described.
...

Dna Integrity Assay (dia) for Cancer Diagnostics, Using Confocal Fluorescence Spectroscopy

The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In
...

Methods for Freparation and Use of Marrow Infiltrating Lymphocytes (mils)

The invention provides compositions comprising activated marrow infiltrating lymphocytes, methods of generating populations of marrow infiltrating lymphcytes, uses of the marrow infiltrating lymphocytes of the invention, and a culture device for use in cell culture, for example for use in generating populations of activated marrow infiltrating lymphocytes. In certain embodiments, the marrow infiltrating lymphocytes can be used as a cancer therapeutic.
...

Stat3 Antagonists and their Uses As Vaccines Against Cancer

The present invention relates to methods for treating and/or preventing cancer. In particular the present invention relates to ex vivo immunotherapeutic methods. The methods comprise decreasing Stat3 (signal transducer and activator of transcirption3) expression and/or function in tumor cells and the administration of such cells to a subject in need of treatment and/or prevention. Other methods of the invention comprise activation T-cells by co-culturing the T-cells with the tumor cells with dec
...

Methods for Identifying Cancer Risk

The present invention provides methods and kits for identifying an increased risk of developing cancer in a subject. The methods include analyzing a first biological sample, such as a blood sample, from the subject for loss of imprinting of the IGF2 gene. According to the methods a loss of imprinting is indicative of an increased risk of developing cancer. The method can include analyzing genomic DNA from the sample for altered methylation of the IGF2 gene. The altered methylation for example in
...

Imidated biopolymer adhesive and hydrogel

Biologically compatible polymers carry an imide and can be used as an adhesive, a hydrogel or both. A second biologically compatible polymer reactive with the imidated polymer can be used therewith to seal openings.
...

Reagents and Methods for Engaging Unique Clonotypic Lymphocyte Receptors

latforms comprising at least one lymphocyte affecting molecule and at least one molecular complex that, when bound to an antigen, engages a unique clonotypic lymphocyte receptor can be used to induce and expand therapeutically useful numbers of specific lymphocyte populations. Antigen presenting platforms comprising a T cell affecting molecule and an antigen presenting complex can induce and expand antigen-specific T cells in the presence of relevant peptides, providing reproducible and economic
...

Transgenic Frog Lines and Assays Employing Them

Rhodopsin transgenes driven by rhodopsin promoters were produced, some attached to GFP coding sequences as a fusion construct. When the resulting transgenes were introduced into Xenopus laevis, the photoreceptors degenerated in a manner similar to the degeneration observed in human retinal degenerations. Lines of animals with these transgenes were generated, and the progeny of such lines undergo similar patterns of degeneration. Since photoreceptors in these generated lines are marked by the exp
...

Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes

Disclosed is a microarray of binary nucleic acid probes for the detection of one or a plurality of nucleic acid analytes in a complex sample in a single high throughput assay with extraordinary specificity under physiologic conditions Binary nucleic acid probes that generate a detectable signal when bound to analyte are suitable for use in the micro arrays, including binary deoxyribozyme or ribozyme probes, nonenzymatic binary probes for fluorescent detection, nonenzymatic binary dye-binding pro
...

Multi-phased, biodegradable and osteointegrative composite scaffold for biological fixation of musculoskeletal soft tissue of bone

Methods and apparatuses are provided for musculoskeletal tissue engineering. For example, a scaffold apparatus is provided which comprises microspheres of selected sizes and/or composition. The microspheres are layered to have a gradient of microsphere sizes and/or compositions. The scaffold provides a functional interface between multiple tissue types.
...

A Split Dna Enzyme for Visual Single Nucleotide Polymorphism Typing

A probe that changes solution color in the presence of only one of the two DNA sequences, which differ by a single nucleotide, is reported. The probe consists of two oligodeoxyribonucleotides, which form a hydrogen peroxidase-like DNA enzyme when hybridized to the abutting fragments of the complementary analyte. The active peroxidase catalyses oxidation of colorless substrates to the colored products. The probe allows visual detection of a mutation in Alzheimer's disease-related DNA.
...

Linked Peptide Fluorogenic Biosensors

Biosensors, compositions comprising biosensors, methods of producing biosensors, and methods of using biosensors are disclosed. The biosensors comprise a fluorogen- activating peptide and a blocking peptide. The fluorogen-activating peptide and blocking peptide are covalently linked through a peptide linker. The blocking peptide associates with the fluorogen-activating peptide thereby blocking an active domain of the fluorogen- activating peptide when the linker is in an unmodified state. The pe
...

Mutants of the P4 Protein of Nontypable Haemophilus Influenzae with Reduced Enzymatic Activity

A P4 variant protein that has reduced enzymatic activity and that induces antibody to wild-type P4 protein and/or has good bactericidal activity against non-typable H. influenzae (NTHi) is useful as an active component in an immunogenic composition for humans. Methods of using these proteins, and compositions containing them in combination with additional antigens, are also provided.
...

Stereochemically Defined Dipeptide Esters of Antiviral Agents for Enhanced Ocular Treatment

Stereochemically defined dipeptide esters of nucleoside-analogous antiviral agents including acyclovir and ganciclovir are provided. Certain of these stereochemically defined dipeptide esters are found to have unexpectedly enhanced delivery to and uptake by ocular tissues, crossing the blood-ocular barrier more effectively than other stereochemically defined dipeptide esters. For example, (L-Val)-(D-Val)-acyclovir was found to be taken up more effectively into corneal tissue than were underivati
...

Plant Artificial Chromosome Platforms Via Telomere Truncation.

The invention provides engineered plant minichromosomes generated by telomere mediated truncation of native chromosomes. These minichromosomes are faithfully transmitted from one generation to the next and provide an ideal platform for breeding genes into desired plant varieties with out problems, such as linkage drag, associated with standard breeding methods.
...

Acyclovir-peptide Analogs

Dipeptide and tripeptide ester derivatives of acyclovir and its analogs are disclosed which are useful to treat herpes virus infections. Also disclosed is a method for preparing a therapeutic agent for targeted delivery to ocular tissue comprising linking the therapeutic agent to one or more groups of the formula -X-Y-Z(n)-R; wherein each X, Y and Z is independently Met, Val, Thr, Tyr, Trp, Ser, Ala or Gly; each R is independently H or an amino-protecting group; and each n is independently 0 or
...

Drought Responsive Genes in Plants and Methods of their Use

Microarrays are employed to analyze soybean transcriptions under water stress conditions in both root and leaf tissues at vegetative stage. Novel drought responsive genes may be thus identified and may be used for enhancing drought tolerance in soybean or other plants through genetic/metabolic engineering. This disclosure pertains to nucleic acid molecules isolated from Soybean that encode polypeptides that may be important for drought tolerance. The disclosure also relates to methods of using t
...

Caffold for Bone and Tissue Repair in Mammals

A mammalian tissue scaffold and method for making a tissue scaffold including a rigid scaffold body of biocompatible glass fibers bonded together and in special alignment to define open channels within the scaffold to allow fluid flow into and within the scaffold.
...

Method for detecting DNA methylation in cancer cells

The present invention provides a detecting method of detecting malignant cells in a patient's specimen or a biological sample. Specifically, the inventive method includes the steps of extracting a genomic DNA, digesting said genomic DNA with one or multiple methylation sensitive restriction enzymes, and amplifying by PCR with one or multiple selected primers. The PCR can be performed in a conventional or a real-time platform. The inventive method can detect leukemia cells in 90% ALL patients at
...

Assays for Detecting Pregnancy- Associated Glycoproteins

Disclosed are methods and compositions for detecting pregnancy in an animal by means of assaying peptidase activity of one or more Pregnancy Associated Glycoproteins (PAGs). In certain aspects, methods also comprising use of an antibody that binds immunologically to a PAG that displays proteolytic activity are provided. Substrates of proteolytic PAGs are also provided, as are kits, and methods of use. Further, methods of purifying PAGs based on their proteolytic activity are also provided.
...

Phage Display Selection of Anti Fungal Peptides

A method for the identification of peptides having an affinity for the surface of fungi is provided as is a method for the identification of peptides capable of affecting the development of a fungus. Also provided are compositions containing peptides identified using the method of the present invention. In addition, isolated polynucleotides, vectors, expression cassettes and transformed cells capable of expressing peptides identified by the method of the present invention are provided.
...

Novel Inhibitors of Retroviral Reverse Transcriptase

Disclosed are nucleic acid molecules, and methods of their use, which have a specific structure including a double helical domain and a G-quadruplex domain physically connected by a linker domain which may be nucleosidic or non-nucleosidic. These aptamers demonstrate potent inhibition of phylogenetically diverse primate lentiviral reverse transcriptases, which effect is largely independent of aptamer sequence provided that the aptamer has the specified structure.
...

Modulating levels of RNA-binding proteins for the treatment of breast cancer

The present invention relates to methods of using RNA-binding protein modulating agents to treat of cancer, particularly patients that are susceptible to or diagnosed with estrogen receptor-negative breast cancer, such as methods of inhibiting the growth or metastasis of cancer cells comprising contacting cells with a therapeutically-effective amount of an HuR-modulating agent. The invention also relates to compositions comprising therapeutically-effective amounts of an HuR-modulating agent.
...

Self-assembling Multicellular Bodies and Methods of Producing a Three-dimensional Biological Structure Using the Same

Structures and methods for tissue engineering include a multicellular body including a plurality of living cells. A plurality of multicellular bodies can be arranged in a pattern and allowed to fuse to form an engineered tissue. The arrangement can include filler bodies including a biocompatible material that resists migration and ingrowth of cells from the multicellular bodies and that is resistant to adherence of cells to it. Three-dimensional constructs can be assembled by printing or otherwi
...

Scaffolds with Trace Element for Tissue Regeneration in Mammals

A scaffold for implantation into a mammal to facilitate vessel growth in repair, regeneration, and/or proliferation of bodily tissue, where the scaffold is based on a borate, silicate, or phosphate, glass-former and is biodegradable upon implantation in mammals. The scaffold includes one or more trace elements from the group consisting of Cu, F, Fe, Mn, Mo, Ni, Sr, and Zn which are released into the host to support vessel growth. A method involves implantation of such scaffolds.
...

Controlling Vessel Growth and Directionality in Mammals and Implantable Material

A method for directing vessel growth toward a blood-deficient site in a mammal comprising implanting into the mammal an assembly of at glass fibers to form a vascular bridge with a first end of the vascular bridge in contact with the blood-deficient site and a second end of the vascular bridge remote from the blood-deficient site. Over time the bridge biodegrades and promotes vascularity in the direction of the bridge.
...

Scaffold for Tissue Regeneration in Mammals

A three-dimensional scaffold with interconnected pores for repair of tissue comprising a scaffold body for structural support of the tissue scaffold, where the scaffold body comprises scaffold body components bonded to each other and made from component materials comprising about 40 to about 90 wt% B2O3, and two or more other oxides, wherein the scaffold body has a porosity between about 15 and about 90 vol%.
...

Method to Develop High Oleic Acid Soybeans Using Conventional Soybean Breeding Techniques

Disclosed is a soybean plant with mutations in FAD2-1A and FAD2-1B. Moreover, disclosure is directed to seeds from said plants with altered ratios of monosaturated and polyunsaturated fats In particular, the disclosure is directed to plants which exhibit elevated levels of oleic acid.
...

Soybean Transcription Factors and Other Genes and Methods of their Use

Gene expression is controlled at the transcriptional level by very diverse group of proteins called transcription factors (TFs). 5671 soybean (Glycine max) genes have been identified and disclosed as putative transcription factors through mining of soybean genome sequences. Distinct classes of the TFs are also disclosed which may be expressed and or function in a manner that is tissue specific, developmental stage specific, biotic and/or abiotic stress specific. Manipulation and/or genetic engin
...

Autism-associated Biomarkers and Uses Thereof

Biomarkers for human autism are disclosed. Methods for treating, preventing, and diagnosing human autism and autism-related disorders are also disclosed.
...

Uses of Immunologically Modified Scaffold for Tissue Prevascularization Cell Transplantation

This invention provides method of making and using of a porous 3 dimensional cyclic RGD peptide-modified alginate scaffold that can be loaded with different cell types and/or growth factors for implantation at sites of tissue damage to promote tissue regeneration. The cyclic RGD peptide promotes vascular formation of the host tissue, cell binding and survival of seeded cells. Scaffolds with growth factors but without cells can also be implanted to create a vascular bed in which cells are transpl
...

Enzymatic Degradation of A2e and Other Bisretinoid Compounds of Rpe Lipofuscin

The present invention provides methods for reducing the amount of lipofuscin compounds present in a cell which comprises contacting the cell with an amount of an enzyme sufficient to directly or indirectly degrade the amount of lipofuscin compounds, so as to reduce the amount of lipofuscin compounds, wherein the enzyme is a carotenoid cleaving dioxygenase or an oxo-iron heme-based enzyme. The invention also provides methods for. treating a subject suffering from a lipofuscin- associated disorder
...

Recombinant Human Follicle Stimulating Hormone Antagonists and Methods of Use Thereof

In one embodiment the present invention is directed to a recombinant human follicle stimulating hormone (FSH) antagonist having reduced N-linked glycosylation. In other embodiments, the present invention is directed to compositions comprising such FSH antagonists, such as combination compositions. In other embodiments the invention provides methods of treatment, including methods of treating ovarian cancer, and methods of increasing the efficacy of a chemotherapeutic agent, such as cisplatin.
...

Compositions of Humanized Notch Fusion Proteins and Methods of Treatment.

This invention provides a fusion protein comprising a signal peptide, an extracellular domain of human Notch receptor protein and an Fc portion of an antibody bound thereto. This invention also provides a method for treating a subject having a tumor, a method for inhibiting angiogenesis in a subject, a method for treating a subject having ovarian cancer, and a method for treating a subject having a metabolic disorder, comprising administering to the subject an amount of the above fusion protein
...

Systems and Methods for Inducing Electric Field Pulses in a Body Organ

Systems and methods for providing controllable pulse parameter magnetic stimulation are described. One aspect is directed to a magnetic stimulation system for inducing approximately rectangular electric field pulses in a body organ, comprising an electrical energy storage device, a stimulating coil, and a switching means for electrically coupling said electrical energy storage device to said stimulating coil to produce current pulses in said stimulating coil which generates, in response to the c
...

Methods for Inducing Transplantation Tolerance

A method is provided for preventing rejection by an immune system of a recipient subject of a tissue transplanted from a donor subject into the recipient subject without the need for long-term administration of non-specific immunosuppressive drugs.
...

Gab2 Amplification in Melanoma

Provided herein are methods of detecting or inhibiting melanoma growth based on Gab2 protein expression. Gab2 protein was found to be either amplified and/or overexpressed in melanoma. Gab2 protein expression correlated with clinical melanoma progression and higher levels of expression were seen in metastatic melanomas compared to primary melanoma and melanocytic nevi. Over-expression of Gab2 potentiates, whereas silencing of Gab2 reduces, migration and invasion of melanoma cells. Gab2 mediated
...

Herpes Virus Backbone for Viral Vaccine and Vaccine Based Thereon

A recombinant deoxyribonucleic acid comprising a human herpes simplex virus (HSV) deoxyribonucleic acid (DNA) having a heterologous DNA integrated therein wherein the heterologous DNA encodes a polypeptide comprising a RING-finger domain; a recombinant virus comprising such, a viral vaccine and methods of immunization are provided.
...

Osteochondral Implants, Arthroplasty Methods, Devices, and Systems

Implants for resurfacing or repairing one or more articular cartilage bearing surfaces of a biological organism include an engineered tissue and a biocompatible porous substrate secured to the engineered tissue for attaching the implant to a native bone of the biological organism. The engineered tissue includes a scaffold containing a biocompatible material, and a plurality of living chondrocytes supported by the scaffold. Methods for culturing chondrocytes for incorporation into a biocompatible
...

Fibroblast Derived Stem Cells

The present invention provides methods and compositions relating to the production of stem cells, derived from dedifferentiated fibroblasts, and the use of such stem cells for treatment of a variety of different disorders and conditions. The invention is based on the surprising discovery that a population of stem cells, capable of differentiating into a variety of different cell types, can be generated by culturing fibroblasts under selective culture conditions.
...

Massive parallel method for decoding DNA and RNA

This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the -OH group at the 3'-position of the deoxyribose.
...

Systems and Methods of Blood-based Therapies Having a Microfluidic Membraneless Exchange Device

The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety o
...

Assay for Determining Health of Cd8+ T Cells

A method of determining if a subject's CD8+ T-cells functionally recognize an HLA-E/Hsp60sp target structure comppsmg a) contacting a sample of the subject's CD8+ T-cells with a HLA-E+ cell loaded with Hsp60sp, b) quantifying proliferation of the contacted HLA-E+ cell, c) contacting a sample of the subject's CD8+ T-cells with a HLA-E+ cell which is loaded with a peptide which does not bind to HLA- E, d) quantifying proliferation of the HLA-E+ cell loaded with the peptide which does not bind to H
...

Microbubble Devices, Methods and Systems

A hydrogel tissue engineering scaffold having microbubbles dispersed therein is disclosed. Also, a system for cell culturing including a controller and actuator to apply dynamic deformational loading to a hydrogel is disclosed. Also disclosed are methods for producing hydrogels with microbubbles and for culturing cells using hydrogels with microbubbles.
...

Ligand Binding Site of Rage and Uses Thereof

The present method provides for an isolated peptide having an amino acid sequence corresponding to the amino acid sequence of a V-domain of a receptor for advanced glycation end product (RAGE). The present invention also provides for an isolated peptide having an amino acid sequence A-Q-N-I-T-A-R-I-G-E-P-L-V-L-K-C-K-G-A-P-K-K-P-P-Q-R-L-E-W-K (Seq. ID No. 1). The present invention provides for a pharmaceutical composition comprising a therapeutically effect amount of an isolated peptide having an
...

Universal Methylation Profiling Methods

This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransf erase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns. This invention also provides neobases and methods of sequencing for methylation patterns using neobases.
...

Delivery of Dna or Rna Via Gap Junctions from Host Cells to Target Cells and a Cell-Based Delivery System for Antisense or siRna

A method of delivering an oligonucleotide or a plasmid expressing an oligonucleotide into a target cell comprises introducing an oligonucleotide into a donor cell, particularly a stem cell, and contacting the target cell with the donor cell under conditions permitting the donor cell to form a gap junction with the target cell, whereby the oligonucleotide or a product of the oligonucleotide is delivered into the target cell from the donor cell.
...

Cancer Stem Cells, Kits, and Methods

The present invention provides a cancer stem cell that has, inter alia, the properties of asymmetrical cell division, tumor initiating capacity, and metastatic competence. This cancer stem cell population is phenotypically characterized by the lack of major histocompatibility antigens and differentiation markers (e.g., cytokeratins, androgen receptor, etc.) and evidence of activation of self-renewal/developmental pathways (e.g., WNT/ss-catenin, NOTCH and Hedgehog). The identification of this can
...

Transgenic mice over-expressing receptor for advanced glycation endproduct (RAGE) in brain and uses thereof

The present invention provides for a transgenic non-human animal whose cells contain a DNA sequence comprising: (a) a nerve tissue specific promoter; and (b) a DNA sequence which encodes a receptor for advanced glycation endproducts (RAGE), wherein the promoter and the DNA sequence which encodes the receptor for advanced glycation endproducts (RAGE) are operatively linked to each other and integrated in the genome of the non-human animal, and wherein said non-human animal exhibits a reduced amou
...

Control of Spore Germination

Provided is a method of stimulating germination of a spore of a Gram-positive bacterium. Also provided is a method of inhibiting germination of a spore of a Gram-positive bacterium. Additionally provided is a composition comprising (i) a preparation of cell walls from a Gram-positive bacterium or (ii) a compound that stimulates activity of a serine/threonine protein kinase of the Gram-positive bacterium or (iii) a compound that inhibits activity of a PPM-like phosphatase of the Gram-positive bac
...

Compensating for Atrioventricular Block Using a Nucleic Acid Encoding a Sodium Channel Or Gap Junction Protein

A method of increasing the velocity of AV conduction in a mammal that may be in heart block or at risk of heart block by causing, in an AV node and/or His bundle having less than normal conduction speed, the expression of a sodium channel or gap junction protein, such as the SkM-I channel, Cx43 or Cx32, so as to increase the velocity of conduction by the AV node.
...

Method for assaying protein-protein interaction

The invention relates to a method for determining if a test compound, or a mix of compounds, modulates the interaction between two proteins of interest. The determination is made possible via the use of two recombinant molecules, one of which contains the first protein, a cleavage site for a proteolytic molecule, and an activator of a gene. The second recombinant molecule includes the second protein and the proteolytic molecule. If the test compound binds to the first protein, a reaction is init
...

Methods for predicting pregnancy outcome in a subject by HCG assay

The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present inve
...

Histone Acetyltransferase Activators and Uses Thereof

The invention provides for a method for screening compounds that bind to and modulate a histone acetyltransferase protein. The invention further provides methods for treating neurodegenerative disorders, conditions associated with accumulated amyloid-beta peptide deposits, Tau protein levels, and/or accumulations of alpha-synuclein as well as cancer by administering a HAT-activating compound to a subject.
...

Nucleic acids for inhibiting hairless protein expression and methods of use thereof

The present invention provides DNAzymes and ribozymes that specifically leave Hairless Protein mRNA. The present invention also provides antisense oligonucleotides that specifically inhibit translation of Hairless Protein mRNA. The invention also provides various methods of inhibiting the expression of Hairless Protein. Finally the invention provides pharmaceutical compositions containing the instant DNAzymes, ribozymes and antisense oligonucleotides as active ingredient.
...

Bioreactor, Devices, Systems and Methods

Disclosed are bioreactor devices, systems and methods. A bioreactor system can include one or more bioreactor modules that can be individually controllable and identifiable. A bioreactor module can be connected to one or more functional modules such as a pump module, a stimulation signal generation module, a motor module, a mechanical transmission module, a gas exchange module, a temperature module, a humidity module and/or a CO2 module, among others. The bioreactor and functional modules can in
...

Systems and Methods of Blood-based Therapies Having a Microfluidic Membraneless Exchange Device

The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety o
...

Medical Implants, Prostheses, Prosthesis Parts, Medical Instruments, Devices and Auxiliary Contrivances Made of a Halogenide-modified Magnesium Substance

The use of a magnesium substance, whose corrosiveness is altered as a result of modification with halogenides, enables medical implants, prostheses or prosthesis parts and medical instruments, devices and auxiliary contrivances, especially surgical instruments or tools, for use in and on the human or animal body to be produced, whereby the degree of corrosion-resistance thereof can be adjusted to full corrosion-resistance.
...

Fluorogenic Substrates for Protein Phosphatases and Assays Incorporating the Substrates

Provided is a class of fluorogenic protein phosphatase substrates. These fluorogenic substrates comprises a carbamate moiety having a carbamate functional group other than hydrogen bonded to the carbamate nitrogen, a fluorophore containing a fluorophore functional group able to covalently bond to the oxygen of the carbamate moiety, and a peptide sequence bonded at its N-terminus to the carbon of the carbamate moiety and composed of one or more amino acids in which the N-terminal amino acid is ei
...

Long Term Potentiation with Cyclic-glur6 Analogs

This invention discloses CN2097-like compositions that facilitate the induction of long-term potentiation (LTP). In one embodiment the method comprises inducing long-term potentiation in a subject by the method of administering a therapeutically effective dose of a CN2907-like compound.
...

Intein-based Fluorescent Bio-circuit for Vitamin D Detection

This invention is directed to an intein-based vitamin D fluorescent biosensor that accurately and rapidly measures la,25-hydroxyvitamin D3. Measurement at picomolar quantities is noted. Particular reference is made to the use of Escherichia coli (E. coli) as a sensor organism, although yeast and mammalian host organisms are also contemplated.
...

Detection of Short Rna Sequences

An assay for detection of short sequences of RNA in a synthetic or clinically isolated sample is presented herein. Particular reference is made to detecting RNA based pathogens, such as H5 influenza.
...

Simultaneous Quantitative Multiple Primer Detection of Clostridium Difficile

This invention comprises a multiplex-capable oligonucleotide which is capable of hybridizing to at least one of the C. difficile tcdB, tcdC, or cdtB genes, wherein, wherein said primer consists of a sequence selected from the group consisting of SEQ ID NOS: 1 through 9, or a sequence that exhibits no more than one substitution of a base to a sequence selected from the group consisting of SEQ ID NOS: 1 through 9 and method for polymerase chain reaction (PCR) determining of the presence of a toxig
...

Methods of Detecting Sources of Microorganism Contamination

Methods of detecting a source of a microbial contamination in a suspect sample include detecting at least one member selected from the group consisting of a microbial geosmin synthase, a microbial 2-methylisoborneol synthase and a microbial 2-methylgeranyl diphosphate synthase in the suspect sample. The method can include conducting a nucleic acid amplification assay in the presence of at least one member selected from the group consisting of at least one microbial geosmin primer and at least on
...

Device and Non-toxic Compositions and Methods for Permeabilization of Drosophila Embryos

The invention provides compositions and methods for permeabilizing insect embryos by removing the waxy layer of the shell using a solution containing a non-toxic cyclic terpene and a non-toxic surfactant, preferably a non-ionic surfactant. The invention further provides kits to practice the method of the invention. The invention also provides methods for toxicology and other high throughput screening method including the compositions and methods of embryo permeabilization provided herein.
...

Predicting Hemostatic Risk

Featured are methods for assessing hemostatic risk including the risk for ACS. Such methods include acquiring blood/plasma composition based on a biological sample obtained from a subject, determining parameters associated with blood clotting, simulating in silico blood clotting using the determined parameters and comparing the results of such simulation to a reference and to determine the hemostatic risk from said comparing. In further embodiments, such methods further include selecting a treat
...

Methods of Detection of Factor Xia and Tissue Factor

The invention provides compositions and methods for the detection of Factor XIa or Tissue Factor (TF) activity in a sample using an antibody based clotting time prolongation assay. The invention provides methods for detection of FXIa or TF activity in a sample using a fluorogenic substrate. Further provided herein is a correlation between elevated levels of FXIa and/or TF with inflammation, acute coronary syndrome (ACS), myocardial infarction, coronary artery disease (CAD), heart failure, aortic
...

Co-Administration of a Thrombolytic and an Anti-CD18 Antibody in Stroke

A method for improving clinical outcome in focal ischemic stroke in a mammal by increasing cerebral blood flow and/or reducing infarct size is described which involves administering an effective amount of an anti-CD18 antibody to the mammal, in the absence of removal of the arterial obstruction.
...

Methods and Products Relating to Gsk3beta Regulation

The invention relates to methods and compositions for regulation of GSK3[beta] activity. The invention provides phosphorylated GSK3[beta] polypeptides and antibodies that recognize such polypeptides. The invention further includes methods for treating disorders that are associated with elevated or reduced GSK3[beta] activity.
...

Use of Antibody Secreting Cell Elispot to Assess Antibody Responses Following Antigen Exposure

Disclosed are methods and kits for early detection of antigen exposure through the presence or absence of antigen-specific antibodies.
...

Two-dimensional Photonic Bandgap Structures for Ultrahigh-sensitivity Biosensing

The present invention relates to two-dimensional photonic crystal arrays and their use in biological sensor chips, including those in the form of microfluidic devices. Methods of making the two-dimensional photonic crystals and biological sensor chips are described herein, as are uses of these devices to detect biological targets in samples.
...

Nucleic Acid Binding Compounds and Methods of Use

The present invention relates to homo- and hetero-dimer compounds formed by a disulfide, sulfinyl thio, or olefin bond between two monomers. A method of making a homo- or hetero-dimer compound is also disclosed. The present invention also relates to monomer compounds capable of forming homo- or hetero- dimer compounds, as well as oligomers formed via linkage of one or more dimers. Also disclosed are methods of inhibiting the activity of target RNA molecules, particularly those having a secondary
...

Use of Non-nucleophilic Additives for Reduction of Surface Morphological Anomalies in Probe Arrays

The present invention relates to a formulations and methods for coupling a reactant (or probe precursor) to a functionalized surface for purposes of forming an arrayed sensor. This method includes the steps of: providing a surface having a reactive functional group; and introducing onto the surface, at a plurality of discrete locations, two or more compositions of the invention, which include a different reactant (probe precursor) and a non-nucleophilic additive, wherein such introduction is car
...

Nurr1 Interacting Protein (nuip)

Provided herein are methods of promoting the activity of Nurr1 in a cell comprising contacting the cell with NuIP or an analog or fragment thereof. Also provided are methods of treating or preventing a condition associated with reduced dopaminergic function in a subject, comprising administering to the subject NuIP or an analog or fragment thereof. Methods of inhibiting the activity of Nurr1 in a cell comprising contacting the cell with a NuIP inhibitor are provided. Methods of screening for age
...

Non-androgen dependent roles for androgen receptor in liver cancer

Disclosed are compositions and methods for modulating AR activity, such as non-androgen dependent AR activity. Also disclosed are compositions and methods for diagnosing beast cancer and for inhibiting liver cancer growth. In addition, disclosed are methods for identifying molecules that inhibit AR in non-androgen dependent ways.
...

Automated placental measurement

A method for analyzing the placenta and histology slides of placental tissue comprising: selecting a placental sample to be analyzed; obtaining a digital image of the placental sample; and performing an analysis on the digital image, wherein a mathematical algorithm is applied to the digital image. The results of the analysis are correlated with data on health outcomes in infants, children, or adults and are used to assess future physiological risks to a patient.
...

Gene Differentially Expressed in Breast and Bladder Cancer and Encoded Polypeptides

The present invention relates to a novel human gene that is differentially expressed in human carcinoma. More specifically, the present invention relates to methods of treating or preventing a disorder in a subject. The invention further relates to uses of C35 polypeptides in immunogenic compositions or vaccines, to induce antibody or T cell-mediated immunity against target cells, such as tumor cells, that express the C35 gene. The present invention further relates to use of C35 polypeptides in
...

Optical Detection and Monitoring of Cancer Using a Protein Biomarker

A method of detecting and staging pre-invasive and invasive cervical cancer is disclosed. A cervical tissue is illuminated in vivo with an incident light. Spectral emissions from the cervical tissue are measured at first and second emission wavelengths, with at least one emission wavelength being where PDIP38 is known to autofluoresce. The measured spectral emissions may be processed by subtracting known emissions from one or more non-PDIP38 targets from the spectral emissions. An emissions rati
...

Nogo-Receptors and Methods of Use

Disclosed are compositions relating to the Nogo receptor (NgR) family as well as fragments, chimeras, and variants thereof. The invention provides polypeptides, nucleic acids, vectors, expression systems, and antibodies and antibody fragments related to the NgRs as well as uses thereof. Such uses include modulation neurite outgrowth in a subject and treatment of central nervous system disorders in a subject, as well as, methods of identifying and screening compounds that can be used for modulati
...

Methods and Compositions Related to Synergistic Responses to Oncogenic Mutations

Disclosed are compositions and methods related to new targets for cancer treatment.
...

Continuous flow chamber device for separation, concentration, and/or purification of cells

The present invention relates to methods and apparatuses for cell separation. In particular, the invention relates to separation of a particular cell type from a mixture of different cell types based on the differential rolling property of the particular cell type on a substrate coated with molecules that exhibits adhesive property with the particular cell type. This technology is adaptable for use in implantable shunts and devices for cell trafficking or tumor neutralization.
...

Altered ospa of borrelia burgdorferi

Provided herein are OspA polypeptides from Lyme Disease-causing Borrelia having certain alteration(s). In one embodiment, the alteration(s) increase the conformational stability of the OspA polypeptide containing the alteration(s) while maintaining at least some of the antigenicity of the corresponding unaltered OspA polypeptide. In another embodiment, the altered OspA polypeptide has reduced cross-reactivity to hLFA-1, as compared to the corresponding unaltered OspA polypeptide.
...

Chromosomal Rearrangement

Materials and methods for the detection and mapping of chromosomal rearrangements in a biological sample are provided. In general, the assay involves the synthesis of detectably labeled nucleic acid extension products off the DNA template provided by the biological sample followed by hybridization of those products to an array.
...

Helper Virus-Free Herpesvirus Amplicon Particles and Uses Thereof

The invention features new helper virus-free methods for making herpesvirus amplicon particles that can be used in immunotherapies, including those for treating any number of infectious diseases and cancers (including chronic lymphocytic leukemia, other cancers in which blood cells become malignant, lymphomas (e.g. Hodgkin's lymphoma or non-Hodgkin's type lymphomas). Described herein are methods of making helper virus-free HSV amplicon particles; cells that contain those particles (e.g., packagi
...

Recombinant Factor Viii Having Increased Stability.

The present invention relates to a recombinant factor VIII that includes one or more mutations that result in enhanced stability of both factor VIII and factor VIIIa. Methods of making and using the recombinant factor VlIl, and pharmaceutical compositions containing the same are also disclosed. The present invention further relates to an isolated nucleic acid molecule that encodes the recombinant factor VIII, as well as DNA expression systems and host cells containing the isolated nucleic acid m
...

Dc-stamp Antibodies

Provided herein are antibodies that specifically bind an epitope of DC-STAMP. Specifically, provided herein are monoclonal antibodies that bind an epitope of DC-STAMP, wherein the epitope comprises the amino acid sequence Glu-Val-His-Leu-Lys-Leu-His-Gly-Glu-Lys-Gln-Gly-Thr-Gln (SEQ ID NO:1). Optionally, the epitope comprises the amino acid sequence His-Gly-Glu-Lys-Gln-Gly-Thr-Gln (SEQ ID NO:2). Optionally, the epitope comprises the amino acid sequence Lys-Gln-Gly-Thr-Gln (SEQ ID NO:3).
...

Ultrasound Technology to Control the Spatial Organization of Cells and Proteins in Engineered Tissues

The present invention is directed to methods of inducing spatial organization of cells an in vitro culture system using ultrasound technology. The invention is further directed to methods of inducing extracellular matrix remodeling and neovessel formation in an in vitro culture system and generating vascularized engineered tissue constructs using ultrasound technology.
...

Spatially Inhomogenously Functionalized Porous Media and Method for Use in Selective Removal of Contaminants

Compositions and methods for separating double-stranded nucleic acids out of a mixture comprising single-stranded nucleic acids and/or dNTPs and/or enzymes. The method uses spatially inhomogenously functionalized nanoporous materials. For example, the compositions and methods of the present invention can be used to purify DNA amplification reaction products.
...

Compositions and Methods for Inhibiting G Protein Signaling

Disclosed are compositions and methods for treating diseases associated with G protein [beta][gamma] subunit activity.
...

Animal Model for Osteoarthritis and Intervertebral Disc Disease

Provided herein is a transgenic animal whose genome comprises a first nucleic acid sequence encoding a fusion polypeptide, wherein the fusion polypeptide comprises a Cre recombinase and a mutated ligand binding domain of human estrogen receptor (CreER), wherein the first nucleic acid is operably linked to a chondrocyte-specific promoter and a second nucleic acid sequence encoding a ss-catenin polypeptide, wherein the second nucleic acid sequence comprises one or more loxP sequences. Also provide
...

Mlk Inhibitors and Methods of Use

Provided are compounds having an inhibitory effect on Mixed Lineage Kinases. Also provided are pharmaceutical compositions, methods of preparing the compounds, synthetic intermediates, and methods of using the compounds, independently or in combination with other therapeutic agents, for treating diseases and conditions which are affected by Mixed Lineage Kinase inhibition. Also provided are methods of treatment of neuropsychiatric disorders which comprise the inhibition of Mixed Lineage Kinases.
...

Preventing and Treating Sepsis

Provided are polypeptides comprising a variant activated protein C comprising one or more amino acid substitutions selected from the group consisting of K146R, D172N, C212R, K146G, R147G, R177G and combinations thereof. Also provided are nucleic acids encoding the polypeptides, and cells, compositions and kits containing the polypeptides and nucleic acids. Also provided are methods of treating sepsis in a subject comprising administering to the subject one or more of the provided polypeptides or
...

Soluble Low-density Lipoprotein Receptor Related Protein Binds Directly to Alzheimer's Amyloid-beta Peptide

A soluble derivative of low-density lipoprotein receptor related protein-1 (sLRP-1) binds directly to Alzheimer's amyloid-[beta] peptide (A[beta]). This binding may be used to detect A[beta] or to separate A[beta] from the rest of a subject's body. In Alzheimer's disease, it may be used to provide diagnostic results by detecting A[beta], treatment by removing A[beta], or both.
...

Enhancing the Therapeutic Effect of Acupuncture with Adenosine

The present invention relates to a method of improving the therapeutic effect of acupuncture in a subject. The method involves administering adenosine, an adenosine mimetric, an adenosine modulator, an adenosine transport inhibitor, enzymes involved in adenosine metabolism, and/or an adenosine receptor agonist to the subject under conditions effective to improve the therapeutic effect of the acupuncture.
...

Production of Human Papillomavirus Capsid Protein and Virus-like Particles

The present invention is directed to a method of expressing the papillomavirus capsid protein coding sequence in a cell using an expression system under conditions facilitating expression of the protein in the cell. In another aspect of the invention, it has been discovered that virus-like particle(s) (VLPs), fragment(s), capsomer(s) or portion(s) thereof are formed from the papillomavirus capsid protein. It was further discovered that the virus-like particle(s) comprises antigenic characteristi
...

Psa and Klk2 As Therapeutic Targets and Molecules Inhibiting Psa and Klk2

Disclosed herein are compositions and methods relating to prostate-specific antigen (PSA), KLK2 and androgen receptor. Further provided are methods and compositions for inhibiting PSA and/or KLK2 activity. Further provided are compositions and methods for treating or preventing cancer.
...

Thioredoxin Interacting Protein (TXNIP) As Regulator of Vascular Function

The present invention relates to screening assays for the identification of agents that can modify the interaction of thioredoxin interacting protein (TXNEP) on thioredoxin (TRX)5 preferably by inhibiting TXNIP downregulation of TXR. The use of such compounds, including the disclosed siRNA and antibodies against TXNIP, is contemplated for therapeutic or prophylactic treatment of vascular disease conditions, particularly those associated with pro-inflammatory activity of the TNF- ASK1-JNK-p38 pat
...

Fibroblast Growth Factor (fgf) Analogs and Uses Thereof

The invention relates to novel peptide comprising FGF-P and methods of use thereof.
...

Treating Myelin Diseases with Optimized Cell Preparations

The disclosure relates to oligodendrocyte-biased glial progenitor cells and methods of making, isolating, and using such cells.
...

Tumor Suppression Through Plexin C1

Disclosed are compositions and methods relating to Plexin C1 and diagnosing, treating, and evaluating treatment for cancer.
...

Methods of Treating B-cell Cancers

The present invention is directed to methods of causing malignant B-cells and treating malignant B-cells. These methods involve the use of an inhibitor of PKK activity, whether active directly against PKK or effective to knockdown PKK expression, which when introduced into a malignant B cell (or administered to a patient) is effective to cause cell death of the malignant B cell, thereby treating the B-cell malignancy.
...

Use of Protein Tyrosine Inhibitor Such As Genistein in Treating Diabetic Retinopathy Or Ocular Inflammation

PROBLEM TO BE SOLVED: To provide a method for prophylactically and therapeutically treating diabetic retinopathy and a method for prophylactically and therapeutically treating ocular inflammation. ; SOLUTION: Administration of an inhibitor of the protein tyrosine kinase pathway to an animal (e.g. a mammal, particularly a human) in an amount sufficient to treat diabetic retinopathy or ocular inflammation is provided. The inhibitor of the protein tyrosine kinase pathway is preferably genistein or
...

Ablation Based Laser Machining of Biomolecule Patterns on Substrates

A method for patterning a one or more biomolecules on a substrate that includes coating the substrate with a coating of the one or more biomolecules, applying a laser to the coating, and ablating a portion of the one or more biomolecules with the laser in a predetermined pattern.
...

Compositions and Methods for Ex Vivo Hepatic Nucleic Acid Delivery

The invention provides compositions and methods for delivery of nucleic acids to the liver ex vivo. The method includes slow, low pressure infusion of the nucleic acid into the liver to efficiently transduce cells with minimal cell damage.
...

A Novel in Vitro Hiv-1 Latency Model for Screening Reactivation Agents of Hiv-1

In one aspect, the present invention provides an isolated Bcl-2-transduced cell that comprises, integrated into the genome of the cell, a transcription-competent immunodeficiency virus or a transcription-competent immunodeficiency virus-based retroviral vector. Under basal in vitro culture conditions, the immunodeficiency virus is latent, and the expression of the latent immunodeficiency virus can be reactivated. The present invention further provides screening methods for identifying agents cap
...

Detecting and Sorting Methylated Dna Using a Synthetic Nanopore

Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. T
...

Annexina2 As Immunological Target

AnnexinA2 (ANXA2), a member of the Annexin family of calcium-dependent, phospholipid binding proteins, is one of a panel of identified antigens recognized by the post-vaccination sera of patients who demonstrated prolonged disease-free survival following multiple vaccinations. AnnexinA2 is abundantly expressed in pancreatic adenocarcinomas and cell surface/membrane AnnexinA2 increases with the progression from premalignant lesions to invasive pancreatic adenocarcinomas. The cytoplasmic to cell s
...

Cells for Increased Protein Expression Comprising One Or More Release Factors and Methods of Use

The invention provides recombinant cells or recombinant restrictive cells for overexpressing one or more proteins comprising one or more release factors. The invention also provides methods for producing a recombinant protein comprising culturing the cells of the present invention under conditions such that one or more proteins are overexpressed, and then isolating the expressed recombinant protein, In certain embodiments of the present invention, the gene encoding the protein is codon optimized
...

Hypoxia Induced Mitogenic Factor

We found that FIZZ1/RELM[alpha] is inducible by hypoxia in lung. The hypoxia-upregulated expression of FIZZ1/RELM[alpha] was located in the pulmonary vasculature, bronchial epithelial cells, and type II pneumocytes. Recombinant FIZZ1/RELM[alpha] protein stimulates rat pulmonary microvascular smooth muscle cell (RPSM) proliferation dose-dependently. Therefore, we renamed this gene as hypoxia-induced mitogenic factor (HIMF). HIMF strongly activated Akt phosphorylation. The phosphatidylinositol 3-k
...

Leucine-rich repeat kinase (LRRK2) drosophila model for parkinson's disease: wildtype1 (WT1) and G2019S mutant flies

Mutations in the leucine-rich repeat kinase (LRRK2) gene cause late-onset autosomal dominant Parkinson's disease (PD) with pleiomorphic pathology. Previously, we and others found that expression of mutant LRRK2 causes neuronal degeneration in cell culture. Here we used the GAL4/UAS system to generate transgenic Drosophila expressing either wild-type (WT1) human LRRK2 or LRRK2-G2019S, the most common mutation associated with PD. Expression of either WT1 human LRRK2 or LRRK2-G2019S in the photorec
...

Parstatin Peptides and Uses Thereof

The invention provides parstatin peptides, particularly a mammalian parstatin peptide including amino acids 1-26 of full length mammalian parstatin, preferably a human parstatin. The invention provides parstatin peptides in appropriate pharmaceutical carriers and formulated for administration. The invention provides for the use of the peptide for example as a medicament or for the preparation of a medicament. The invention provides methods of use for parstatin peptides including for inhibition o
...

Methods of Treating Brain Tumors with Antibodies

The application is directed toward a method of treating a brain tumor in a patient comprising systemically administering a monoclonal antibody.
...

Multicomponent Degradable Cationic Polymers

Degradable polymers were synthesized that self-assemble with DNA to form particles that are effective for gene delivery. Small changes to polymer synthesis conditions, particle formulation conditions, and polymer structure provides significant changes to efficacy in a cell-type dependent manner. Polymers presented here are more effective than commercially available materials, such as LIPOFECTAMINE 2000TM, FUGENE TM , or polyethylenimine (PEI), for gene delivery to cancerous fibroblasts or human
...

Single Molecule Spectroscopy for Analysis of Cell-free Nucleic Acid Biomarkers

The present invention relates, e.g., to a method for detecting a nucleic acid molecule of interest in a sample comprising cell-free nucleic acids, comprising fluorescently labeling the nucleic acid molecule of interest, by specifically binding a fluorescently labeled nanosensor or probe to the nucleic acid of interest, or by enzymatically incorporating a fluorescent probe or dye into the nucleic acid of interest, illuminating the fluorescently labeled nucleic acid molecule, causing it to emit fl
...

Methylated CpG Island Amplification (MCA)

The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated PcG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers. A meth
...

Cytokine Receptor Chain

PROBLEM TO BE SOLVED: To provide a receptor fragment having an affinity to IL-13, a method for producing the same, and the treating use of the same. ; SOLUTION: A polynucleotide having a specific sequence encoding the IL-13 receptor and its fragments, a receptor protein of the IL-13, a method for producing the same, an inhibitor of the bonding of the IL-13 with its receptor, a method for identifying the same and a method for a medical treatment by using such molecule and an antagonist for the IL
...

Cancer Stem Cell Expression Patterns and Compounds to Target Cancer Stem Cells

Described herein are therapeutic targets expressed in cancer stem cells and methods for treating and diagnosing cancer by targeting such cells with antibodies, compounds, nucleic acid, or other therapeutic agent. In one embodiment described herein, therapeutic agents for the treatment of cancer are provided based on the identification of cancer stem cell targets. The present invention also includes therapeutic targets for cancer therapy and cancer stem cell-targeted therapy. The invention includ
...

Socs-1 Gene Methylation in Cancer

Methods are provided for identifying a cell exhibiting unregulated growth associated with methylation-silenced transcription of a suppressor of cytokine signaling (SOCS)/cytokine-inducible SH2 protein (CIS) family member (SOCS/CIS) gene such as the SOCS-1 gene. In addition, methods of treating a cancer patient, wherein cancer cells in the patient exhibit methylation-silenced transcription of SOCS/CIS gene such as a SOCS-1 gene, are provided, as are reagents for practicing such methods.
...

Antibodies that Specifically Bind Hedgehog-Derived Polypeptides

The present invention provides two novel polypeptides, referred to as the "N" and "C" fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G'CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog
...

Co-polymer Stabilizers for Hydrofluoroalkane Dispersions

The use of biodegradable and biocompatible lactide-based nonionic amphiphiles to stabilize a model drug (salbutamol base) dispersion in hydrofluoroalkane (HFA) propellant is disclosed. The results have industrial applicability to the development of HFA-based dispersion pressurized metered-dose inhaler formulations.
...

Activated T Cells

Provided are compositions comprising activated T cells and activated T cells armed with bispecific antibodies directed at cancer antigens which are poor responders to alloantigen, and methods of using the compositions to help engraftment and improve anti-tumor effects.
...

Method and Composition for a Protein Transduction Technology and Its Applications

A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins usi
...

2,2-Bis-(hydroxymethyl)cyclopropylidenemethyl-Purines and -Pyrimidines As Antiviral Agents

Compounds which are active against viruses have the following formulas: wherein B is a purine or pyrimidine heterocyclic ring or base. In a preferred embodiment, the purine include 6-aminopurine (adenine), 6-hydroxypurine (hypoxanthine), 2-amino-6-hydroxypurine (guanine), 2,6-diamino-purine, 2-amino-6-azidopurine, 2-amino-6-halo substituted purines such as 2-amino-6-chloropurine, 2-amino-6-fluoropurine, 2-amino-6-alkoxypurines such as 2-amino-6-methoxypurine, 2-amino-6-cyclopropylaminopurine, 2-
...

Genus-wide Chlamydial Peptide Vaccine Antigens

Peptides generated from a random library that are bound by a monoclonal antibody to Chlamydial glycolipid exoantigen (GLXA) and thus mimic this antigen are disclosed. Peptides that correspond to antigen-binding regions of an anti-idiotypic antibody (mAb2) specific for anti-GLXA antibody (Ab1) which act as molecular mimics of GLXA are also disclosed used as immunogens to induce broadly reactive genus-specific anti-chlamydial antibodies. These peptides and immunogenic DNA encoding the mAb2-like pe
...

Biomarkers for Diagnostic and Therapeutic Methods

Erythrocyte ATP-release modulators and composition and methods for their use as biomarkers of glucose processing or vascular disorders, as well as methods for screening to identify to modulators; methods for monitoring efficacy of therapy; and apparatus for use in such methods.
...

Galectin-3 cleavage as a marker for matrix metalloproteinase activity in cancer

Provided are differential antibodies recognizing the cleaved and non-cleaved forms of matrix metalloproteinases (MMPs), and methods of using the antibodies as surrogate diagnostic markers for the presence of active MMPs in cancer, such as growing breast cancers.
...

Therapeutic Amides

The invention provides compounds of the formula: wherein X, Y, and Z are as defined in the specification. The compounds are effective anti-tumor agents. The invention also provides pharmaceutical compositions comprising a compound of the above formula or a salt thereof, intermediates useful for preparing a compound of the above formula, and therapeutic methods comprising administering a compound of the above formula or a salt thereof to a mammal in need thereof.
...

Modified Egfr Ectodomain

A protein that attenuates EGFR and/or EGFR family members comprises a modified EGFR ectodomain. The protein inhibits signaling via the EGFR and/or EGFR family members. The protein includes a portion of the EGFR (or EGFR family member) and the "U" region epitope of EGFR related protein (ERRP), wherein the portion of the EGFR is operable to bind a ligand of EGFR. Also included are nucleic acids encoding such proteins. Attenuating EGFR signaling can include inhibiting the EGFR and/or EGFR family me
...

Cyclic-glur6 Analogs, Methods of Treatment and Use

A composition which is reversible inhibitor of at least one neuron-specific PDZ domain comprising wherein structure (5), R is a molecular transporter with or without a linker amino acid; R1 is at least about one amino acid covalently bound; and, R2 is isoleucine, leucine, alanine, phenylalanine, or valine, and methods of use.
...

Hybrid 2-aminoterailin and aryl-substituted piperazine compounds and their use in altering CNS activity

Hybrid compounds containing an aminotetralin moiety or a heterocyclic and/or open chain analog thereof linked through an alkylene group to an aryl ring system-substituted piperidiene moiety exhibit high levels of CNS activity, in some cases exhibiting especially high relative binding efficiencies between D3 and D2 dopaminergic receptor subtypes.
...

Polyamines Useful As Anti-parasitic and Anti-cancer Therapeutics and As Lysine-specific Demethylase Inhibitors

Polyamine, polyamine/guanidino, and polyamine/biguanide compounds are disclosed. The compounds are useful as anti-cancer and anti-parasitic treatments. The compounds are also useful as inhibitors of the enzyme lysine-specific demethylase-1.
...

Method for Identifying a Subpopulation of Mammalian Cells with Distinctive Ribosome Translation Profiles

The present invention generally relates to subpopulations of mammalian cells with distinctive ribosome translational profiles, i.e. translational activities. The present invention further relates to methods for identifying and isolating such cells, kits comprising the same, or methods which utilize different translational activities of these subpopulations of mammalian cells.
...

Non-Natural Peptides as Models for the Development of Antibiotics

Described herein are methods of screening one of the RNA hairpins in the small ribosomal subunit of bacteria to identify peptides that bind to it. The RNA hairpin target may be the 970 loop (aka helix 31 (h31)) or a modified version thereof. The identified peptides may inhibit protein synthesis and, therefore, may be used as a model for new antibiotics.
...

Engineering Porous Particles of Water Soluble Therapeutics for Pressurized Metered-dose Inhaler Formulations

The subject matter disclosed herein relates to methods for engineering porous particles of water soluble therapeutics with enhanced physical stability and aerosol characteristics in hydrofluoroalkane (HFA)-based pressurized metered-dose inhaler (pMDI) formulations.
...

Homogeneous distribution of cellular suspension in porous carrier material for tissue engineering under using molded body with chamber having opening, comprises introducing the carrier material provid

The process for homogeneous distribution of a cellular suspension in a porous carrier material (3) for tissue engineering under using a molded body (1) with a chamber (2) having an opening for receiving the carrier material, comprises introducing the carrier material provided for colonization into the chamber. (contd)
...

Process for the Preparation of Cyclodextrins Composed of More Than Eight Glucose Units

A process for the preparation of large-ring cyclodextrins (LR-CD) with a degree of polymerisation (DP) of 9 - 21 from starch is described. LR-CD are obtained in a biocatalytic process in high yields by controlling the temperature and reaction time. The LR-CD mixtures obtained are separated from glucose and others reaction products in a precipitation process. After removal of smaller CD by complexation reactions enhanced by an ultrasonication treatment, CD9 to CD21 can then be obtained in high pu
...

Promotion of Peroxisomal Catalase Function in Cells

The molecular mechanisms of peroxisome biogenesis have begun to emerge; in contrast, relatively little is known about how the organelle functions as cells age. The present inventors characterized age-related changes in peroxisomes of human cells and showed that aging compromises peroxisomal targeting signal 1 (PTS1) protein import, with the critical antioxidant enzyme, catalase, especially affected. The number and appearance of peroxisomes are altered in these cells, and the organelles accumulat
...

Polyclonal Bispecific Antibody Compositions and Method of Use

The invention relates to compositions and methods for treating a host infected with a pathogen. The composition comprising a population of polyclonal bispecific binding molecules that can target and eliminate a host cell infected with the pathogen. Methods for activating and arming cytotoxic immune cells with the composition for use in treating a patient infected with a pathogen are also provided.
...

Compositions and Methods for Detecting and Modulating Cell Death By a Translation Regulated Gene Expression System

The technology relates to a nucleic acid expression cassette comprising a TR element encoding an mRNA molecule that is translated in stressed and/or dying cells, and a nucleotide sequence operably linked to the TR element, that is a first open reading frame (ORF) sequence and encodes a polypeptide or a fragment thereof and is co-translated with the TR element. The technology further relates to mammalian cells and a transgenic animal comprising such expression cassette. Further included are kits
...

Anti-cancer Therapeutic Strategy to Overcome Cancer Resistance and to Enable Tailoring Treatment to Patients

A gene construct comprising a programmed-cell-death executioner gene having a nuclear localization signal, a deleted signal peptide, an actin-resistant binding site, a promoter, and an activator. A method of making a gene construct, by modifying a programmed-cell-death executioner gene by adding a nuclear localization signal, deleting a signal peptide, mutating a binding site for an inhibitor to make it actin- resistant, adding a promoter for exclusive expression in selected cells, and adding an
...

Therapeutic Compositions and Methods for Delivery of Active Agents Cleavably Linked to Nanoparticles

Various embodiments of the present invention pertain to therapeutic compositions that comprise: (1) an active agent (e.g., paclitaxel); and (2) a nanoparticle (e.g., gold nanoparticle). In such embodiments, the active agent is covalently linked to the nanoparticle through a cleavable linker (e.g., a linker containing a hyadrazone species). Other embodiments of the present invention pertain to methods of treating a condition in a subject by administering the above-described therapeutic compositio
...

Methods of Fabricating Enhanced Tissue-engineered Cartilage

Compositions and methods for fabricating a tissue-engineered cartilage construct comprising: providing a cell sample comprising a plurality of chondrocytes; culturing the cell sample to produce a tissue-engineered cartilage construct; and treating the tissue-engineered cartilage construct, wherein treating the tissue-engineered cartilage construct comprises the use of a biochemical reagent, a mechanical force, hydrostatic pressure, or any combination thereof.
...

Systems and Methods for Magnetic Guidance and Patterning of Cells and Materials

Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method
...

Systems and Methods for Producing Plastid Proteins

Methods and systems that include a method comprising: providing a system comprising one or more plastid proteins comprising a signal sequence and one or more chloroplast processing enzymes; and allowing at least one of the one or more chloroplast processing enzymes to cleave at least a portion of a signal sequence from at least one of the one or more plastid proteins.
...

Spermicidal and Microbicidal Compositions

Provided herein are new spermicidal compositions that include one or more fluorous surfactants, e.g., non-ionic fluorous surfactants, anionic fluorous surfactants, cationic fluorous surfactants, and combinations thereof. Also provided are methods of preventing fertilization of an ovum, which include a step of contacting at least one spermatozoon with one or more fluorous surfactants. Additionally described herein are methods of preventing infection, which include a step of contacting at least on
...

Bisphosphonate-prostatic Acid Phosphatase Inhibitor Conjugates to Treat Prostate Cancer Bone Metastasis

The present invention concerns conjugate compounds comprising a bisphosphonate covalently bonded to a prostatic acid phosphatase inhibitor and compositions comprising such conjugates. Methods for treating and inhibiting prostate cancer bone metastases, and determining whether a conjugate is useful for such treatment are also provided. In some instances, the bisphosphonate is alendronate, and it is covalently bonded to either tartaric acid or glyceric acid.
...

Serum Markers Associated with Early and Other Stages of Breast Cancer

Methods for identifying disease-specific markers, in particular breast cancer markers, by electrophoretically separating serum albumin complexes in a biological sample on a membrane are provided. Electrophoretic separation profiles representing different diseases or different cancer stages can be produced, and used in the diagnosis, prognosis and treatment of these diseases. Methods for identification of a cancer peptide fragment comprising a cancer peptide motif are provided. Also provided are
...

Composite Bone Grafts, Particulate Bone-calcium Sulfate Constructs, and Methods of Treating Joint Injuries

A solid implantable bone construct (12) shaped like a cylinder, a cone, or a frustum, for anchoring ligament implants. The bone construct (12) can include a bone component and a biocompatible solid component. The bone component can include particulate bone of between 75 and 600 microns, powdered bone of 75 microns or smaller in size, or both. The biocompatible solid component can include calcium sulfate hemihydrate, a calcium phosphate product, or both. The bone component can be between 5 and 50
...

Antibacterial Peptides Active in Systemic Infections

Antimicrobial peptides of the formula are provided: X1-X2-PrO- X3- X4-PrO- Arg- Pro-Tyr-Leu-Pro- X5-Pro-Arg-Pro-Pro-Arg-Pro-Y, wherein X1 is a natural or non-natural amino acid having a free amino group or 1-amino-cyclohexyl carboxylic acid, X2 is Arg or N-methyl-Arg, X3 is Asp or GIu, X4 is Arg or Lys, X5 is Arg or Lys, and Y is Arg, Arg- NH2, N-methyl-Arg, N-methyl-Arg - NH2, Val-Arg, Val-Arg- NH2, Val-(N-methyl- Arg), or Val-(N-methyl)-Arg- NH2, and salts thereof.
...

Manipulating Sp1 Activity to Improve Therapeutic Cloning

The observed over-expression of Sp1 target genes has inspired inventors to formulate a specific strategy for correcting many gene expression defects, and thus improve clone development. The invention is based on the belief that manipulating Sp1 activity can improve cloning. Inventors believe that cloning is inefficient in large part because of the continued expression of Sp1 target genes in the early cloned embryos, which causes clones to be very unlike normal embryos, and so makes them very unh
...

An Assay for Screening/ranking Antioxidants Based on their Effects on Membrane Sterol Lateral Organization

A method of screening an antioxidant for potency and/or toxicity in vitro, the method includes contacting the antioxidant with a model system comprising a sterol superlattice formation capable of generating a detectible signal, wherein the detectible signal changes in a parameter representative of an integrity of the sterol superlattice formation; and detecting and/or measuring disruption of the sterol superlattice formation, wherein the disruption is caused by oxidation of sterol upon contactin
...

Use of Nibp Polypeptides

Methods for regulating NF-?B activation in cells comprising introducing into the cell a vector comprising a nucleic acid sequence encoding a NIK and IKK2 Binding Protein (NIBP) polypeptide, wherein the NIBP polypeptide is expressed in the cell, are provided. Also provided are methods for reversing the cancerous phenotype of a cancer cell and for modulating neuronal differentiation.
...

Triggerably Dissolvable Hollow Fibers for Controlled Delivery

Provided are tubular structures of a biocompatible, triggerably-dissolvable material such as cellulose or a copolymer having an LCST below physiological temperatures. The structures may be embedded within a cell growth scaffold. The tubular structures are useful in growing 3-dimensional tissue structures because nutrients, cytokines or other cell growth and/or differentiation compounds, as well as drugs, such as antibiotics and steroids, can be administered over time, and the tubular structures
...

Biosensors and Related Methods

Provided herein are biosensors that comprise a biological signal source linked to a substrate by a peptide nucleic acid spacer and methods of use of the biosensor. In one embodiment, the biosensor is used to detect prostate-specific antigen.
...

Micrornas in Idiopathic Pulmonary Fibrosis

The present invention relates to the discovery that certain microRNAs are differentially expressed in Idiopathic Pulmonary Fibrosis. The present invention provides for diagnostic methods, therapeutic methods, and kits related to these differentially expressed microRNAs.
...

Nitro Fatty Acids As Regiomers and Related Mimetics

Mimetics of nitro linoleic acid are potent agonists of PPAR-?, a receptor that regulates adipocyte differentiation, glucose homeostasis and insulin sensitivity. Thus, 10-LNO2 and 12-LN-NO2 are potent, selective activators of the PPAR-?, as are mimetics of LNO2 that bind in a manner similar to LNO2. High-resolution crystallographic data on the binding of LNO2 by PPAR-? thus inform the design of such mimetics.
...

Polypeptides and Uses Thereof

The invention provides a polypeptide having a sequence comprising from 10 to 24 consecutive amino acids from residues 215-233 of the sequence of human cyclin B1 protein or homolog thereof, as well as a nucleic acid molecule encoding the polypeptide, and compositions thereof. The invention also provides a method for using such polypeptides, nucleic acid molecules, or combinations thereof for treatment or prophylaxis of cancer in patients.
...

Cytotoxic T Cell Defined Egfr Peptide and an Optimized Derivative Peptide

The invention provides a polypeptide having a sequence of amino acids consisting of IXDFGLAKL (SEQ ID NO: 1), as well as a nucleic acid encoding the polypeptide, vector comprising the nucleic acid, cell comprising the vector, and compositions thereof. The invention also provides a method of inducing a T-cell response in a patient with epithelial cancer, and a method inhibiting epithelial cancer, wherein the methods comprise administering the composition of the invention.
...

Novel Biosensors for Live Cell Imaging of Intracellular Proteins

This invention is related to the field of imaging intracellular protein processing. Fluorescent probes capable of changing color over time are used to identify and track the synthesis, trafficking, and secretion of intracellular proteins. One such probe useful in this invention is a monomeric multifluorescent protein, specifically a mcTimer probe as a biosensor for secretory proteins. After expression, mcTimer sequentially changes from green to red over a twenty- four hour period. In particular,
...

Targeting an Hiv-1 Nef-host Cell Kinase Complex

Drug candidates for inhibition of HIV-I replication can target Src family kinases (SFK), such as Hck, that interact with Nef protein of the virus. Compounds characterized by such inhibitory activity were identified via an assay for kinase activity of an SFK in a Nef:SFK complex. Illustrative of inhibitors identified using the kinase assay are various 2,3- diaminoquinaxolines and furo[2,3-d]pyrimidines. The inventive inhibitors were found to arrest HIV-I viral replication in vitro.
...

Cancer Stem Cell Immortalization

The present invention relates to the preparation and use of immortalized cancer stem cells. The immortalized cancer stem cells of the invention may be used in assays to identify anti-cancer compounds as well as molecules critical to carcinogenesis. Further, cancer stem cells may be harvested from a patient and used, according to the invention, to select agents more likely to be effective in treating the cancer of that particular patient.
...

Early Prostate Cancer Antigen-2: a Novel Serum Specific Marker for Prostate Cancer Detection

Early Prostate Cancer Antigen-2 (EPCA-2) is a prostate cancer biomarker, newly identified via proteomics-based examination of alterations in nuclear structure. EPCA-2 shows a higher overall specificity than PSA. Thus, an immunoassay based on antibodies against an epitope of EPCA-2 more accurately differentiates between confined and non-confined disease than PSA or Gleason score.
...

Substance P and Analogs Thereof As a Cancer Immunogenic Composition Adjuvant

Disclosed herein are methods and compositions effective for treating, preventing, and ameliorating cancer that utilize Substance P or Substance P analogs and antigens. Also disclosed are methods for vaccinating a patient against cancer that utilize Substance P or Substance P analogs and antigens.
...

Methods of Labeling Biological Samples

Methods are provided for labeling specific oxidized proteins. Methods also are provided for determining the oxidation state of a cell. Such methods are useful as diagnostic, therapeutic and screening agents.
...

Adipose-derived Stem Cells and Lattices

The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The cells can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the cells can be expanded and cultured to produce hormones and to provide c
...

Cell Line

The invention provides a cell line complementing HSV ICP4, ICP27, and UL55 genes.
...

Therapeutic and Diagnostic Cloned Mhc-unrestricted Receptor Specific for the Muc1 Tumor Associated Antigen

The invention provides an isolated nucleic acid encoding a receptor, other than an immunoglobulin, wherein the receptor binds to a MUC1 tumor antigen independently of an major histocompatibility complex (MHC). The invention provides a method of activating a signaling pathway and/or killing a cancer cell using a receptor that is similar to or is a T cell receptor
...

Artificial Cell Constructs for Inducing Immunological Tolerance

The present invention contemplates induction of immunological tolerance thereby providing permanent allograft acceptance. This method obviates the need for a lifelong regimen of immunosuppressive agents which can increase the risk of infection, autoimmunity, and cancer. Immunological tolerance is thought to be mediated by regulatory T lymphocytes (Treg cells) with immunosuppressive capabilities. A therapeutically relevant platform comprising artificial constructs are contemplated comprising nume
...

Human Leukocyte Antigen Alleles Associated with Advanced Lung Diseases

The present invention relates to methods and compositions that may be used to predict the risk of an individual, for example a smoker, for developing chronic obstructive pulmonary disease ("COPD"), emphysema or idiopathic pulmonary fibrosis ("IPF"). The invention particularly relates to methods of evaluating the risk of an individual developing COPD or IPF comprising the detection of one or more of the Cw*07, Cw*0702, HLA-B*07, HLA-B*0702, HLA-A*03, DRB 1 * 1501, DQB 1 *0602, Cw*05, Cw*08, or Cw
...

Thermoresponsive, Biodegradable, Elastomeric Material and Uses Therefor

Provided are novel biocompatible copolymers and compositions comprising the copolymers. The copolymers are non-toxic and typically have an LCST below 37 DEG C. Compositions comprising the copolymers can be used for wound treatment, as a cellular growth matrix or niche and for injection into cardiac tissue to repair and mechanically support damaged tissue. The copolymers comprise numerous ester linkages so that the copolymers are erodeable in situ. Degradation products of the copolymers are solub
...

Small Molecule Inhibitors of Dusp6 and Uses Therefor

Compounds that stimulate fibroblast growth factor production, and thus cell growth are provided. Also provided are compositions comprising the compounds and methods of using the compounds. The compounds can be used to treat wounds, to expand cell populations, such as hematopoietic cells, or to grow tissue in vitro, among other uses.
...

Linked Myc-max Small Molecule Inhibitors

Provided herein are compounds and compositions for interfering with the association of Myc and Max. These compounds and compositions are useful in methods for inhibiting growth or proliferation of a cell. Methods of inhibiting growth or proliferation of a cell comprise contacting the cell with an amount of a compound that interferes with Myc and Max association effective to inhibit growth or proliferation of the cell.
...

Biologic Scaffold for Prevention of Pulmonary Fibrosis

Provided herein are methods of preventing, lessening or treating pulmonary fibrosis in a subject. The methods comprise delivering an amount of a powdered extracellular matrix (ECM)-derived material to the respiratory system of the subject effective to prevent, lessen or treat pulmonary fibrosis in a subject. Also provided is an apparatus for delivering the powdered ECM-derived material to a subject.
...

Muscle Derived Cells (mdcs) for Promoting and Enhancing Nerve Repair and Regeneration

The present invention describes methods involving the use of muscle derived cells (MDCs), preferably obtained from skeletal muscle, to support the innervation and repair of damaged tissues and organs, particularly associated with nerve damage or neuropathy. The invention relates to MDCs for use in methods for promoting or enhancing innervation of nerve cells, particularly in the peripheral nervous system, and their ability to contribute to the development of neuronal tissue when MDCs are introdu
...

Fstl-1 As a Biomaker of Inflammation

The invention provides methods and kits for diagnosing severity of particular types of inflammatory diseases, such as rheumatic diseases, by assessing protein levels of follistatin-like protein 1 (FSTL-1). The level of FSTL-1 protein present in the serum or synovial fluid of individuals suspected of having certain inflammatory disease (e.g, rheumatoid arthritis) is positively correlated with the severity of the disease.
...

Identification of markers in esophageal cancer

Methods for identifying expression of markers indicative of the presence of esophageal, a squamous cell cancer, a squamous cell cancer of the head and neck, colon cancer and melanoma are provided. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods.
...

Bone Augmentation Utilizing Muscle-derived Progenitor Compositions in Biocompatible Matrix, and Treatments Thereof

The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment non-soft tissue following introduction (e.g. via injection, transplantation, or implantation) into a site of non- soft tissue (e.g. bone) when combined with a biocompatible matrix, preferably SIS. The invention further provides methods of using compositions comprising muscle-derived progenitor cells with a biocompatible matrix for the augme
...

Targeted Nitroxide Agents

Provided herein are compositions and related methods useful for free radical scavenging, with particular selectivity for mitochondria. The compounds comprise a nitroxide-containing group attached to a mitochondria-targeting group. The compounds can be cross-linked into dimers without loss of activity. Also provided herein are methods, for preventing, mitigating and treating damage caused by radiation. The method comprises delivering a compound, as described herein, to a patient in an amount and
...

Biomarkers for Prognosis of Pulmonary Diseases

The present invention relates to biomarkers that may be used to evaluate the prognoses of patients suffering from pulmonary diseases and assist in the determination of appropriate therapeutic regimens. It is based, at least in part, on the discovery that a number of T-cell antigens are differentially expressed in chronic lung disease patients depending on the prognosis of the patient. Non-limiting examples of these antigens include CD28, CD4, CD25, CD45, CD27 and CCR7 and combinations thereof. U
...

Bioerodible Wraps and Uses Therefor

A tubular tissue graft device is provided comprising a tubular member and a restrictive fiber matrix of a bioerodible polymer about a circumference of the tubular tissue. The matrix may be electrospun onto the tubular tissue. In one embodiment, the tubular tissue is from a vein, such as a saphenous vein, useful as an arterial graft, for example and without limitation, in a coronary artery bypass procedure. 5 Also provided is method of preparing a tubular graft comprising depositing a fiber matri
...

Porous Biomolecule-Containing Metal-Organic Frameworks

The present invention relates to compositions including porous biomolecule-containing metal-organic frameworks and methods for their preparation. The porous biomolecule-containing metal-organic frameworks can include a metal component and a biomolecule component. The pores located within the frameworks have a pore space and said pore space is capable to adsorb materials therein. These compositions of the present invention are useful in a wide variety of applications, such as, but not limited to,
...

Oxygen Depletion Devices and Methods for Removing Oxygen from Red Blood Cells

An oxygen depletion device. The device has a cartridge; a plurality of hollow fibers extending within the cartridge from an entrance to an exit thereof; an amount of an oxygen scavenger packed within the cartridge and contiguous to and in between the plurality of hollow fibers. The hollow fibers are adapted to receiving and conveying red blood cells. There is another embodiment of an oxygen depletion device and method for removing oxygen from red blood cells.
...

Engineered Microparticles for Macromolecule Delivery

A method for making a modified release composition, comprising: selecting a desired active agent and polymer matrix for formulating into a modified release composition; assessing degradation effect on release of the active agent from the composition including plotting polymer molecular weight (Mwr) at onset of active agent release vs. active agent molecular weight (MwA); predicting performance of multiple potential formulations for the composition based on the degradation assessment and average
...

Detection of Nucleic Acids Using a Cantilever Sensor

Detection of miniscule amounts of nucleic acid is accomplished via binding of target nucleic acid to probe material, composed of nucleic acid, which is bound to a sensor configured to sense mass. The sensor is prepared by immobilizing a probe material to a surface of the sensor, wherein the probe material is known to bind to the target nucleic acid. The prepared sensor is exposed to the target nucleic acid. The target nucleic acid binds to the probe material. The mass accumulated on the sensor r
...

Electrowetting Microarray Printing System and Methods for Bioactive Tissue Construct Manufacturing

Apparatuses and methods for manufacturing three-dimensional, bioactive, tissue scaffold fabrications with embedded cells and bioactive materials, such as growth factors, using biomimetic structure modeling, solid freeform fabrication, biocompatible hydrogel material, and electrowetting on dielectric-based multi-microarray printing.
...

Bioadhesive Hydrogels

A method of forming and the resulting bioadhesive hydrogel composition for repairing or supplementing a nucleus pulposus of an intervertebral disc comprises at least a first component, a second component and a third component, wherein the first component, the second component and the third component are crosslinked. The first component comprises an amine-containing polymer component at a concentration from about 0.1% weight by weight to about 13.65% weight by weight. The second component compris
...

Encapsulation of Microbubbles Within the Aqueous Core of Microcapsules

The present invention includes a construct that comprises at least one microbubble encapsulated within the aqueous core of a microcapsule. The present invention also includes a pharmaceutical composition comprising a construct comprising at least one microbubble encapsulated within the aqueous core of a microcapsule. The present invention further includes a method of imaging a tissue or and organ in a subject, a method of delivering a therapeutic cargo to a tissue or organ in a subject, and a me
...

Plasma Treatment for Growth Factor Release from Cells and Tissues

Aspects of the present invention are related to methods comprising contacting an endothelial cell in an endothelial cell population with a non-thermal plasma to release an angiogenic growth factor. The released angiogenic growth factor may induce endothelial cell proliferation. In certain embodiments, the angiogenic growth factor is fibroblast growth factor-2. Preferably, the non-thermal plasma may be an atmospheric pressure dielectric barrier discharge. Additional aspects of the present inventi
...

Flow-based Enhancement of Specificity for Label-free Biochemical Assays

A label-free biochemical assay, in which label-free interrogation of a target-receptor layer is performed while the target-receptor layer is subjected to a relatively strong flow of an analyte-containing fluid. The volumetric flow rate for the assay is selected based on calibration data corresponding to the target substance, which advantageously results in fewer and/or smaller false-positive signals corresponding to non-target substances compared to those produced with the fluid being stationary
...

Compositions and Methods for Treating a Disorder Or Defect in Soft Tissue

The present invention encompasses methods and compositions for generating a biomimetic proteoglycan. The invention includes methods of treating a disease, disorder, or condition of soft tissue using a biomimetic proteoglycan.
...

Small Diameter Vascular Graft Produced By a Hybrid Method

The present invention relates to a hybrid graft and methods of generating the hybrid graft. The hybrid graft comprises an exterior surface and a luminal surface. The luminal surface comprises a micropattern of grooves to which cells adhere and orient along. The exterior surface comprises electrospun microfibers wherein the microfibers provide mechanical properties to the graft, The hybrid graft is capable supporting endothelial cell attachment, endothelial cell alignment, cell proliferation, and
...

Phase Transitioning Hydrogels

A method of forming and the resulting hydrogel composition comprising poly( vinyl alcohol) at a final concentration of about 20% (w/w) to about 65% (w/w) and polyethylene glycol at a final concentration of about 2% (w/w) to about 20% (w/w), wherein the hydrogel composition has a total polymer content, above about 30% (w/w), higher than the total polymer content of a precursor polymer solution formulated prior to the formulation of the hydrogel composition. The hydrogel composition may further co
...

Self-assembled proteins and related methods and protein structures

The present invention provides user-directed construction of novel specific homo- and hetero-dimeric, and multimeric assemblages of proteins. The present invention is comprised of gene sequences that transcribe peptide sequences that form links between proteins, where the peptide sequences produce a hook or loop which supports specific self-assembly of homo-dimers, hetero-dimers and multimers of the proteins to which they are attached. The hook or loop may have a short aliphatic repeat sequence
...

Methods of Modulating Immune Function

Presented herein are therapeutic agents that modulate one or more immune functions and uses of such therapeutic agents in the prevention, treatment and management of diseases. In one aspect, the therapeutic agents modulate one or more signal transduction pathways induced by the binding of B7-H7 to B7-H7CR, or the binding of B7-H2 to either ICOS, CD28, or CTLA-4. In another aspect, the therapeutic agents modulate the binding of B7-H7 to B7-H7CR, or the binding of B7-H2 to either ICOS, CD28, or CT
...

Measurement of Mitochondrial Membrane Potential to Assess Organ Dysfunction

The present invention relates to the field of organ transplantation. More specifically, the present invention provides methods for predicting organ function after transplantation. In certain embodiments, the method comprises measuring mitochondrial membrane potential from a biopsy sample from the donor organ. The present invention is also applicable to organ dysfunction in general. More specifically, the methods of the present invention may be useful in formulating prognoses for patients with ac
...

Programmable Multispectral Illumination System for Surgery and Visualization of Light-sensitive Tissues

An observation system for viewing light-sensitive tissue includes an illumination system configured to illuminate the light-sensitive tissue, an imaging system configured to image at least a portion of the light-sensitive tissue upon being illuminated by the illumination system, and an image display system in communication with the imaging system to display an image of the portion of the light-sensitive tissue. The illumination system is configured to illuminate the light-sensitive tissue with a
...

Biomarkers for Myocardial Ischemia

This invention relates, e.g., to a method for determining if a subject has myocardial ischemia, comprising (a) providing a blood sample obtained from a subject suspected of having myocardial ischemia; (b) determining in the sample the amount of one or more of the following proteins: (i) Lumican and/or (ii) Extracellular matrix protein 1 and/or (iii) Carboxypeptidase N; and (c) comparing the amount(s) of the protein(s) to a baseline value that is indicative of the amount of the protein in a subje
...

Compositions and Methods for Implantation of Adipose Tissue and Adipose Tissue Products

The invention provides compositions and methods for the preparation of biocompatible biomaterials from adipose tissue. Biocompatible biomaterials are cellular or acellular biomaterials. The invention further provides methods of use of the biocompatible biomaterials.
...

Potent Immunosuppressive Agents, Derivatives and Uses

Provided herein include compositions, all related stereoisomers as well as pharmaceutically acceptable salts provided as simplified analogs of pateamine A, in which the analogs generally are devoid of the C3-amino and C5-methyl groups, also referred to as desmethyl, desamino-pateamine A. Suitable analogs provide anticancer and antiproliferative effects in vivo and in vitro by a novel drugs mechanism of action described herein for pateamine A, including inhibition of eIF4A-dependent translation i
...

Method for detecting cellular proliferative disorders

A novel T-type calcium channel (CACNA1G) is provided, as are polynucleotides encoding the same. CACNA1G has been implicated in cellular proliferative disorders. More specifically, it has been observed that the methylation state of specific regions within CpG islands associated with the CACNA1G gene correlates with a number of cancerous phenotypes involving a variety of tissue and cell types. Also provided are methods for detecting cellular proliferative disorders by determining the methylation s
...

Induction of Thyroid Iodide-handling Gene Expression in Human Cancers

Dual suppression of the MAP kinase and PI3K/Akt pathways showed synergistic or greatly enhanced anti-melanoma cell effects, compared to suppression of a single pathway, including the inhibition of cell proliferation, transformation and invasion, induction of G0/G1 cell cycle arrest and, importantly, cell apoptosis. Remarkably, suppression of either pathway induces the expression of thyroid iodide-handling genes and dual suppression of the two pathways synergistically and robustly induces express
...

Brain Endothelial Cell Expression Pattern

PROBLEM TO BE SOLVED: To provide new techniques for isolating brain endothelial cells (ECs) and evaluating gene expression patterns to gain a better understanding of brain tumor angiogenesis. ; SOLUTION: When transcripts from brain ECs derived from normal and malignant colorectal tissues are compared with transcripts from non-endothelial cells, genes predominantly expressed in the endothelium are identified. Comparison between normal- and tumor-derived endothelium reveals genes that are specific
...

Biological pacemaker

Disclosed are methods and systems for modulating electrical behavior of cardiac cells. Preferred methods include administering a polynucleotide or cell-based composition that can modulate cardiac contraction to desired levels, i.e., the administered composition functions as a biological pacemaker.
...

Secreted and Cell Surface Genes Expressed in Benign and Malignant Colorectal Tumors

Serial analysis of gene expression (SAGE) was used to identify transcripts encoding secreted or cell-surface proteins that were expressed in benign and malignant tumors of the colorectum. A total of 290,394 tags were analyzed from normal, adenomatous and cancerous colonic epithelium. Of the 21,343 different transcripts observed, 957 were found to be differentially expressed between normal and adenoma or between normal and cancer. Forty-nine transcripts were elevated >/= 20-fold in adenomas, 40 t
...

Use of Biomarkers for Detecting Ovarian Cancer

The present invention relates to a method of qualifying ovarian cancer status in a subject comprising: (a) measuring at least one biomarker in a sample from the subject and (b) correlating the measurement with ovarian cancer status. The invention further relates to kits for qualifying ovarian cancer status in a subject.
...

Biomarker for Identification of Melanoma Tumor Cells

This invention relates to use of neuropilin-2 as a novel biomarker and therapeutic target for melanoma. The presence of neuropilin-2 can be used as a biomarker for diagnosing and detecting individuals suffering from or at risk for developing melanoma. Also described are methods of using neuropilin-2 to capture circulating melanoma cells. The present invention further relates to methods of treating an individual suffering from or at risk for developing melanoma with an agent that inhibits the act
...

Detection of Head and Neck Cancer Using Hypermethylated Gene Detection

Methods and kits for detection of a cell proliferative disorder, such as head and neck cancer are provided utilizing analysis of the methylation state of targeted genes or regulatory regions of genes in a saliva or serum sample are described. The presence of hypermethylation of the genes or their regulatory regions is indicative of the presence, or a stronger possibility of recurrence and or a poorer prognosis in subjects with cancer.
...

Site-specific Modification of Proteins

Methods and compositions for site-specific modification of amino acids, peptides, polypeptides and proteins are disclosed.
...

Compositions and Methods for the Treatment of Ocular Oxidative Stress and Retinitis Pigmentosa

Oxidative damage contributes to cone cell death in retinitis pigmentosa and death of rods, cones, and retinal pigmented epithelial (RPE) cells in ocular oxidative stress related diseases including age-related macular degeneration and retinitis pigmentosa. Oral antioxidants may provide modest benefits, but more efficient ways of preventing oxidative damage are needed. Compositions and methods are provided herein for the prevention, amelioration, and/ or treatment of early or late stage ocular dis
...

Circulating Mutant Dna to Assess Tumor Dynamics

DNA containing somatic mutations is highly tumor specific and thus, in theory, can provide optimum markers. However, the number of circulating mutant gene fragments is small compared to the number of normal circulating DNA fragments, making it difficult to detect and quantify them with the sensitivity required for meaningful clinical use. We apply a highly sensitive approach to quantify circulating tumor DNA (ctDNA) in body samples of patients. Measurements of ctDNA can be used to reliably monit
...

Biomarkers for Melanoma

The present invention relates to methods of determining melanoma status in a subject. The invention also relates to kits for determining melanoma status in a subject. The invention further relates to methods of identifying biomarkers and correlating biomarker expression to melanoma status or stage in a subject.
...

Lateral Flow Device for Diagnosing Microbial Infections

Fungal infections are difficult to diagnose. The most common filamentous fungal infection, aspergillosis, carries with it a high mortality. Culture of the organism is difficult and obtaining samples, e.g., though a lung biopsy, sometimes causes morbidity. Biomarkers that indicate 'early' infection in it development are sought after. One such biomarker is detection of galactomannan (GM), a polysaccharide that is attached to hyphal cell walls and secreted during growth of the organism. Galactomann
...

Hydrogel-grafted Degradable Nerve Guides

The present invention is directed to the compositions and methods of preparing hydrogel-grafted nerve guides for peripheral nerve regeneration. Particularly, the present invention describes the nerve guides and methods for preparation of hydrogel-grafted nerve guides with encapsulated neurotrophic factors and a nanofiber mesh lining the inner surface of the guide. The present invention also provides methods for peripheral nerve repair using these hydrogel-grafted nerve guides.
...

Compositions and Methods for Treatment of Cancer Using Tissue-specific Oncolytic Adenoviruses

The present invention includes the use of a nucleic acid sequence encoding an shRNA to target RNA interference against a cellular factor where such use can enhance oncolytic adenovius replication. The nucleic acid sequence encoding an shRNA can be introduced into an oncolytic adenovius construct via a recombination event, and such nucleic acid sequence encoding an shRNA can reside in either the El region or Fiber region of the oncolytic adenovius construct. In particular, the oncolytic adenovius
...

Albumin-bound Protein/peptide Complex As a Biomarker for Disease

Methods and kits for diagnosis and prognosis using biomarkers comprising albumin-bound protein/peptide complex (ABPPC).
...

Prodrug Activation in Cancer Cells Using Molecular Switches

The present invention features a novel protein engineering strategy by combining the domains of two independent proteins into a molecular switch. The invention features polypeptides comprising a prodrug activating enzyme and a protein that binds a cancer specific marker, polynucleotides encoding the polypeptides, and molecular switches for converting a prodrug into a toxin, comprising the polypeptides. The invention also features methods for converting a prodrug into a toxin, methods for treatin
...

Methods for Promoting Fusion and Reprogramming of Somatic Cells

The invention features methods for reprogramming somatic cells by treating the cells with one or more agents to induce de-differentiation, in particular by targeting demethylase and methyltransferase genes. The invention also features methods of monitoring somatic cell fusion and reprogramming and methods of identifying agents that alter somatic cell fusion and reprogramming. The invention also features reprogrammed cells and kits.
...

Pathways Underlying Pancreatic Tumorigenesis and an Hereditary Pancreatic Cancer Gene

There are currently few therapeutic options for patients with pancreatic cancers and new insights into the pathogenesis of this lethal disease are urgently needed. To this end, we performed a comprehensive analysis of the genes altered in 24 pancreatic tumors. First, we determined the sequences of 23,781 transcripts, representing 20,583 protein-encoding genes, in DNA from these tumors. Second, we searched for homozygous deletions and amplifications using microarrays querying ~one million single
...

Methods and Kits for Identification of Anti-excitotoxic Compounds

The instant invention provides methods and kits for screening for anti-excitotoxic compounds which increase the total amount of EAAT-I protein or the surface amount of EAAT-I protein.
...

Target and Method for Inhibition of Bacterial Rna Polymerase

Target and method for inhibition of bacterial RNA polymerase disclosed are targets and methods for specific binding and inhibition of RNAP from bacterial species.
...

Methods for Obtaining Bioactive Compounds from Phytoplankton

Phytoplankton represent a potential source of bioactive compounds. The present disclosure provides, inter alia, methods for identifying glycerolipids and apoptosis- inducing sphingosine-like lipids from virally-infected phytoplankton.
...

Graft Polymers for Enhanced Intracellular Delivery of Antisense Molecules

Innovative graft polymers designed for the efficient delivery of antisense molecules into biological cells and for maintaining the biological activity of these molecules while in serum and other aqueous environments are provided. Such polymers may comprise an anionic graft polymer comprising an anionic polymer backbone with pendant carboxylic acid groups and pendant chains comprising amphipathic or hydrophilic polymers covalently bonded to a portion of said pendant carboxylic acid groups. Antise
...

Preparation and Uses of Polyarylates

The present invention is directed to polyarylates comprising repeating units having the structure: as well as their preparation and use as cell growth substrates
...

High Molecular Weight Polymers, Devices and Method for Making and Using Same

Anhydride polymers that release active or activatable agent(s) have pre-selected properties such as molecular weight, flexibility, hardness, adhesiveness, and other valuable properties. The polymers are suitable for use in compositions, formulations, coatings, devices, and the like that benefit from the controlled release of an agent(s) over a period of time. The polymers are prepared by a process involving various alternative and sequential steps that allow the design a priori of products with
...

Lithium Stimulation of Cord Blood Stem Cell Proliferation and Growth Factor Production.

The present invention provides methods for expanding human umbilical cord blood stem cells and methods for stimulating growth factor production by cord blood stem cells using an in vitro cell culture system comprising a lithium salt. The present invention also provides in vivo methods for enhancing the survival and growth of transplanted cord blood stem cells by treating the cells with a lithium salt prior to transplantation. In vivo methods for reducing rejection of transplanted cord blood stem
...

Topoisomerase-targeting Agents

The invention provides compounds of formula I or formula II: wherein: the bond represented by <img id="CUSTOM-CHARACTER-00001" he="1.02mm" wi="4.23mm" file="US20090258890A1-20091015-P00001.TIF" img-content="character" img-format="tif"/> is a single bond or a double bond, and R1-R5, X, and Y have any of the meanings defined in the specification and their pharmaceutically acceptable salts. The invention also provides pharmaceutical compositions comprising a compound of formula I or II, processes f
...

Transgenic plants expressing L3 delta proteins are resistant to trichothecene fungal toxins

Disclosed are specific mutants of L3 and transgenic plants that produce them. The plants exhibit increased resistance to fungal toxins that target ribosomal L3 protein. Also disclosed are transgenic plants that co-produce L3 mutant and an RIP protein, and exhibit increased resistance to various fungal toxins and viruses, while reducing toxicity normally associated with production of the RIP. Uses of the L3 mutants in animals are further disclosed.
...

Non-cytotoxic PAP mutants

Disclosed are PAP mutants that are less toxic than wild type PAP and that exhibit depurination activity. Also disclosed are transgenic plants that produce the PAP mutants, and methods for preparing the plants. Further disclosed are bioconjugates containing the PAP mutants, pharmaceutical compositions containing the bioconjugates, and methods of administering the compositions to treat disease.
...

Soybean Oil Based Oxygen Carrying Emulsion for Massive Blood Loss

VivaSure is a new treatment for shock from massive blood loss. It is based on soybean oil and is therefore biodegradable. It refills lost vascular volume, protects from free radicals, absorbs toxins and provides nutrition to cells. Also it can deliver oxygen, nitric oxide or other gases to tissues. Efficacy has been demonstrated in mouse and rat models of severe hemorrhagic shock. In these tests VivaSure was superior to Ringer's Lactate and equal to blood. The market is 25 Billion dollars/year.
...

Toposiomerase Inhibitors

The invention provides compounds of formula (I): wherein A, B, W, Y, Z, R1, R3 and R4 have any of the meanings defined in the specification and their salts. The invention also provides pharmaceutical compositions comprising a compound of the invention, processes for preparing compounds of the invention, intermediates useful for preparing compounds of the invention, and therapeutic methods for treating cancer or a fungal infection using compounds of the invention.
...

Switch-region: Target and Method for Inhibition of Bacterial Rna Polymerase

The invention provides a target and methods for specific binding and inhibition of RNA polymerase from bacterial species. The invention provides methods for identifying agents that bind to a bacterial RNA polymerase, and that inhibit an activity of a bacterial RNA polymerase, through interactions with a bacterial RNA polymerase homologous switch-region amino-acid sequence. Said methods comprise preparing a reaction solution comprising the compound to be tested and an entity containing a bacteria
...

Media Conditioning for Improving Gene Delivery Efficiency to Differentiating Embryonic Stem Cells

The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from ~1.4% to ~3.7% of the population.
...

High-throughput Biomarker Segmentation Utilizing Hierarchical Normalized Cuts

A method and apparatus for obtaining segmented images of the stained regions may comprise quantifying the extent of the presence of staining of a biomarker in an original image of a sample, which may comprise selecting a domain swatch of data based upon a user specified domain knowledge; clustering the data within the original image by conducting a frequency weighted mean shift of the data within the original image to convergence, forming a hierarchical plurality of layers each having a differen
...

Monomers and Phase-separated Biocompatible Polymer Compositions Prepared Therefrom for Medical Uses

The invention relates to novel monomers of Formula (I) useful for preparation of phase-separated biocompatible polymers or polymer compositions. These polymers or polymer compositions may be bioresorbable and/or biodegradable and have desirable mechanical properties, such as fracture and/or fatigue toughness, which have not been a primary design criteria for such polymers previously. The polymers or polymer compositions are useful in a variety of medical applications, such as in the fabrication
...

Synthesis of Cyclic Diguanosine Monophosphate and Thiophosphate Analogs Thereof

The invention provides methods for the synthesis of cyclic dinucleotides and thiophosphate analogs thereof as well as a new family of analogs of cyclic diguanosine monophosphate that includes a series of seven phosphorothioate derivatives that includes diastereomers of mono-, di-, and trithiophosphates.
...

Sterospecific Carbonyl Reductases

Stereospecific carbonyl reductases SCR1, SCR2, and SCR3 are described herein as are nucleotide sequences that encode these reductases. These stereospecific carbonyl reductases have anti-Prelog selectivity and have specificities that are useful for fine biochemical synthesis.
...

Oregano and Mint Anti-inflammatory Compositions and Methods

The present invention relates to bioactivity-guided isolation and identification of bioactive compounds from oregano and mint plants, in particular, rosmarinic acid, oleanolic acid and ursolic acid, and use of these compounds or combinations thereof as anti-inflammatory agents for the treatment of conditions related to pain and inflammation and/or as ingredients of dietary supplements. The invention also relates to optimization of the methods for qualitative and quantitative analysis of the bioa
...

Interspecific cornus hybrid designated KF 111-1

An advanced generation interspecific hybrid of Cornus kousa and C. florida that is distinguished by its exceptionally high vigor, attractive foliage, very floriferous display of white floral bracts, high level of winter hardiness, tolerance of drought and shade, and high resistance to the incitants of Powdery Mildew and Dogwood Anthracnose.
...

Bioresorbable Polymers Synthesized from Monomer Analogs of Natural Metabolites

New bioresorbable polymers are synthesized from monomer analogs of natural metabolites In particular, polymers are polymerized from analogs of amino acids that contribute advantageous synthesis, processing and material properties to the polymers prepared therefrom, including particularly advantageous degradation profiles
...

Disease Resistant Transgenic Plants

Provided are transgenic plants, seed and plant parts which display increased resistance to fungal disease or mycotoxin. Also provided are methods of making transgenic plants with increased resistance to fungal disease or mycotoxin.
...

Methods for Obtaining Bioactive Compounds from Phytoplankton

Phytoplankton represent a potential source of bioactive compounds. The present disclosure provides, inter alia, methods for identifying glycerolipids and apoptosis-inducing sphingosine-like lipids from virally-infected phytoplankton.
...

Combination Therapy to Improve Drug Efficiency

The instant invention provides methods for increasing drug bioavailability and/or preventing multi-drug resistance through inhibition of ABCG2 protein by caffeine or caffeine analogs.
...

Modular Triple-helical Collagen-like Products

Recombinant bacterial triple-helical collagen-like proteins comprising two or more repetitive sequences of Gly-Xaa-Yaa yielding high-stability polymeric constructs without the need for post-translational modifications and which may incorporate one or more functional domains of biological or structural importance. The polymers are capable of high-yield production for a variety of applications
...

Cytokines and Genes Differentially Affected by TNF Blockers

The present invention is directed to cytokines and genes that are differentially affected by TNF blockers and the use of these genes and cytokines to help asses the TB risks of new immunosuppressive therapies, to help evaluate the effects of new TB vaccines, and to help assess TB susceptibility in persons exposed to Mycobacterium tuberculosis.
...

Proteins, Nucleic Acids Encoding the Same, and Associated Methods of Use

Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel h
...

Novel Human Lysosomal Protein and Methods of Its Use

The gene associated and causative of classical late infantile neuronal ceroid lipofuscinosis (LINCL), CLN2, has been identified and characterized. The translation product of this gene is a novel protease and a deficiency in this activity results in LINCL. Identification of CLN2 will not only aid in the prevention of LINCL through genetic counseling but provides strategies and test systems for therapeutic intervention. In addition, further characterization of this previously unknown lysosomal enz
...

Labeled Biomolecular Compositions and Methods for the Production and Uses Thereof

Disclosed herein is a set of E. coli single-protein production (SPP) technologies with protein NMR (SPP-NMR) for (i) using isotope-enriched membrane proteins produced with the SPP system in screening detergent conditions suitable for purification and/or three-dimensional structure analysis without the requirement for protein purification, (ii) producing 2H, 13C, 15N enriched proteins suitable for high throughput and membrane protein NMR studies, and (iii) labeling with 13C-15N specific peptide b
...

Type I Interferon Antagonists

Disclosed in certain embodiments is a method of preparing a Type 1 interferon antagonist comprising modifying a Type 1 interferon at the site of interaction with the interferon receptor subunit IFNAR-1 such that the binding affinity of the interferon to the IFNAR-1 subunit is reduced as compared to the native interferon, and corresponding compositions and methods of treatment thereof.
...

Novel Toxin-antitoxin System

Disclosed in certain embodiments is a method of inhibiting cell function comprising inducing the expression of a mRNA interferase that cleaves mRNA at GCU.
...

Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids

A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable s
...

Salivary Bioassay for Early Detection of Bone Loss

The present invention is directed to methods to detect and diagnose osteoporosis and periodontal disease using salivary biomarkers.
...

Methods and Compositions for Treating Cancer

The present invention provides methods of treating cancer using 2-amino-6-trifluoromethoxybenzothiazole (riluzole). In one aspect, the present invention provides methods of reducing cancer cell growth. In another aspect, the present invention provides a method of inducing apoptosis in a cancer cell. In another aspect, the present invention provides a method of reducing the growth of a glutamate-releasing tumor.
...

Novel Modification of Immunomodulatory Protein

Methods of inhibiting annexin I induced apoptosis by contacting a cell population containing a TRPM7/ChaK1 kinase with an effective amount of a composition containing an inhibitor for the kinase.
...

A Method of Using an Extracellular Matrix to Enhance Cell Transplant Survival and Differentiation

Provided is a matrix for promoting survival and differentiation of cells transplanted thereon, comprising a base matrix and a cell-made matrix thereon. Methods and means for making and using same are also provided.
...

Telomerase Reverse Transcriptase Variant

The present invention relates to nucleic and amino acid sequences of a novel variant of the telomerase reverse transcriptase. More particularly, the present invention is directed to a novel variant of human telomerase reverse transcriptase, which displays properties distinct from those of wildtype telomerase reverse transcriptase, and methods of use thereof.
...

Targeting Bacterial Suicide Pathways for the Development of Novel Antibiotics

The invention provides methods for identifying an agent which prevents or partially prevents an antitoxin from forming a complex with its cognate toxin, comprising contacting a potential agent with a labeled substrate in solution, whereby detection of the label indicates presence of an agent that prevents an antitoxin from forming complex with a toxin. The invention also provides agents capable of interfering with formation of a toxin-antitoxin complex. Such agents act as novel, non-conventional
...

The Use of Protein S Fusion for Protein Solubilization

The invention provides vectors containing a multiple cloning site comprising a PrS tag or a PrS2 tag from Myxococcus xanthus. Methods are provided for enhancing solubility of a target protein using Protein S tagged target fusion proteins. TM KIPO & WIPO 2009
...

Targeting Tumor Cells with Chemotherapeutic Agents Conjugated to Matriptase Antibodies

The present invention relates to matriptase antibodies and immunoconjugates of matriptase antibodies with cytotoxic agents and the use thereof for killing or inhibiting the growth of matriptase-expressing cancer cells, such as those of multiple myeloma and breast cancers. In particular, immunoconjugates comprising a matriptase monoclonal antibody and anticancer agents such as doxorubicin (DOX) are introduced, which are equipotent to anticancer agents used in free form but exhibit significantly r
...

IMAGING INDIVIDUAL mRNA MOLECULES USING MULTIPLE SINGLY LABELED PROBES

A method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, said target sequence including at least 30 non- overlapping probe binding regions of 15-100 nucleotides, comprising immersing said cell in an excess of at least 30 nucleic acid hybridization probes, each singly labeled with the same fluorescent label and each containing a nucleic acid sequence that is complementary to a different probe binding region of said target sequence; wa
...

Compositions Comprising Mg29 Nucleic Acids, Polypeptides and Associated Methods of Use

Disclosed herein are compositions and methods for treatment of muscle dysfunction (including sarcopenia) and other diseases involving skeletal musle, including age-related muscle dysfunction. In addition, the invention relates to therapeutic compositions comprising nucleotides and/or polypeptides of the invention in combination with a pharmaceutically acceptable carrier, wherein the composition facilitates the treatment of skeletal musle disorder, including those related to thr normal aging proc
...

Anti-sense Microrna Expression Vectors

The present invention relates to an alternative strategy for expressing the antisense sequence of a miRNA. This system allows for continuous production of the antisense sequence and subsequently complete knockdown of the targeted miRNA.
...

Immunotherapy for Unresectable Pancreatic Cancer

The present invention provides a novel cancer immunotherapy comprising a vaccination schedule of both intratumoral and systemic injections followed by peripheral boost injection. The immunotherapy can then be followed by other standard treatment as is known in the art for locoregional or metastatic pancreatic cancer. The present invention further provides a kit for administering the cancer immunotherapy described herein. The present invention further provides a method of decreasing the dose of c
...

Multiplex Screening for Pathogenic Hypertrophic Cardiomyopathy Mutations

This invention relates to a new method of screening for hypertrophic cardiomyopathy. In certain embodiments, the invention comprises a method of screening for hypertrophic cardiomyopathy comprising detecting the presence or absence of at least one pathogenic HCM mutation by mutation detection assay in a sample from a subject to be tested for hypertrophic cardiomyopathy.
...

Cold Shock Protein Compositions and Methods and Kits for the Use Thereof

The present invention provides cold shock protein-containing compositions for improved DNA synthesis reactions with improved reactivity, methods for synthesizing DNA using such compositions, kits for use in such methods, and DNA compositions yielded by such methods. The present invention further provides cold shock protein-containing compositions for the identification of endoribonuclease cleavage sites, methods for identifying endoribonuclease cleavage sites using such compositions, and kits fo
...

Compositions and methods for enzymatic detachment of bacterial and fungal biofilms

Isolated nucleic acid sequences and amino acid sequences for soluble -N-acetylglucosaminidase or active fragments or variants thereof which promote detachment of bacterial cells from a biofilm are provided. An isolated mutant bacteria which forms biofilm colonies which tightly adhere to surface but which are unable to release cells into the medium or spread over the surface is also provided. In additions, methods are described for modulating detachment of bacterial cells from biofilm by mutating
...

Tissue Engineered Fibrocartilage Replacement

Fibrocartilage implants characterized by circumferential fiber networks embedded in arcuate or torroidal scaffolds with orthogonal fiber networks embedded therein to prevent separation of the circumferential fiber networks. The fiber networks convert axial compressive forces on the scaffolds to tensile loads on the circumferential fibers. Artificial knee meniscus and vertebral disc implants are disclosed, as well as articular disc implants for joints such as the temporomandibular joint and wrist
...

Chemoprevention of Colorectal Cancer by Mesalamine/Sulfasalazine

A method for preventing or treating colorectal cancer in a patient possessing human tropomyocin isoform TC22 is presented. Also presented is a method for evaluating TC22 expression reduction activity of a compound.
...

Proteins Expressed By Mycobacterium Tuberculosis and Not By Bcg and their Use As Vaccines

The present invention is directed to proteins expressed by Mycobacterium tuberculosis and not by BCG and their use as vaccines.
...

Strategy for Homo- Or Hetero-dimerization of Various Proteins in Solution and in Cell

The present invention describes a chimeric polypeptide comprising a first portion comprising a receptor domain, wherein the receptor domain comprises an intracellular region and a transmembrane region; and a second portion comprising a dimerization domain. The present invention also describes a chimeric polypeptide comprising a first portion comprising a receptor domain, wherein the receptor domain comprises a receptor extracellular region; and a second portion comprising a dimerization domain,
...

Novel Compounds for Inhibiting Eef2 Kinase Activity

The present invention discloses novel compounds for inhibiting eEF2 kinase and methods of use thereof.
...

E2f As a Target for Treatment of Hormone Refractory Prostate Cancer

The instant invention provides amino acid sequences competing with E2F for DNA binding. Methods of using said amino acid sequences for treatment of hormone-refractory prostate cancer are also provided.
...

Structure of a Protein Phosphatase 2a Holoenzyme: Insights Into Tau Dephosphorylation

Embodiments of the present invention relate to crystals and atomic coordinates for PP2A, as well as methods for using these atomic coordinates to prepare modulators of PP2A and inhibitors prepared using such methods. Further embodiments relate to biochemical analyses of the interactions of PP2A alone or in complex with Tau. Further embodiments relate to compositions including mimetics and small molecules, optionally, secondary agents, which may be used to treat disorders in which PP2A activity a
...

Demethylation and Inactivation of Protein Phosphatase 2a

Embodiments of the present invention relate to atomic coordinates for PME-1 alone or in complex with PP2A, as well as methods for using these atomic coordinates to prepare inhibitors of PME-1 and/or PP2A and inhibitors prepared using such methods. Further embodiments relate to biochemical analyses of the interactions of PME-1 alone or in complex with PP2A. Further embodiments relate to compositions including mimetics and small molecules, optionally, secondary agents, which may be used to treat d
...

Nanoparticles for Photodynamic Therapy

Embodiments of the invention include nanoparticulate products of a flash nanoprecipitation process, the products comprising, in combination, pre-formed nanoparticles having properties of an upconversion phosphor and a hydrophobic organic compound having the properties of a photosensitizer, and a method of photodynamic therapy comprising the use of the nanoparticulate products is described.
...

Identification of Bacterial Autoinducer and Use in Treating Bacterial Pathogenicity

A bacterial autoinducer, CAI-I, was purified and its structure identified. Methods for synthesis of the autoinducer and its analogues were elucidated. Methods of using the autoinducer or its analogues for treating bacterial pathogenicity and biofilm formation are described. Methods for prevention and treatment of cholera are described. Synthetic (iS)-3-hydroxytridecan-4-one functions as well as natural CAI-I in repressing production of the virulence factor toxin co-regulated pilus (TCP). Strateg
...

Nano-particle Surface Modification

Oxide, oxysulfide, halide or phosphate host particles with a self-assembled organo- phosphonate monolayer covalently bonded thereto. Methods for coating the host particles and use of rare earth ion-doped particles in imaging methods and photodynamic therapy methods are also disclosed.
...

Modulators of Protein Phosphatase 2a and Pp2a Methyl Esterase

The disclosure relates to modulation of protein phosphorylation, including information derived from the structures and activities of the proteins designated protein phosphatase 2A (PP2A) and PP2A methyl esterase. The disclosure contained herein provides compounds and methods for identification of compounds that antagonize the function of PME, and thus reduce levels of PP2A demethylation activity. Over-expression or gain-of-function of PME contributes to a range of diseases such as cancer, thus i
...

Nanochannel arrays and their preparation and use for high throughput macromolecular analysis

Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.
...

Iap Binding Compounds

Compounds that bind cellular IAPs (inhibitor of apoptosis proteins) are disclosed. The compounds are mimetics of the N-terminal tetrapeptide of IAP-binding proteins, such as Smac/DIABOLO, Hid, Grim and Reaper, which interact with a specific surface groove of IAP. Also disclosed are methods of using these compounds for therapeutic, diagnostic and assay purposes.
...

Inhibitors of Mtor Kinase As Anti-viral Agents

The present invention provides methods and compositions for treating or preventing viral infections using modulators of host cell enzymes relating to mTOR. The invention also provides methods and compositions for treating or preventing viral infections using modulators of host cell enzymes relating to mTOR and modulators of the unfolded protein response.
...

SiRNA PHOSPHOLIPID CONJUGATE

siRNA-conjugated liposomes and micelles, methods of making such conjugates, and methods of using such conjugates, such as for the delivery of siRNA to cells to reduce expression of target polypeptides in such cells, are described.
...

Steroidal Anti-hormone Hybrids

Disclosed are novel compounds and compositions for inhibition of androgen and estrogen receptor signaling, methods for inhibiting androgen signaling, methods for inhibiting estrogen signaling, methods for inhibiting the interaction between a co-regulatory protein and an androgen or estrogen receptor, and methods for treating cancer.
...

Mixed Micelles Including Amphipathic Conjugates of Rna Agents, and Uses Thereof

Disclosed are improved pharmaceutical formulations for the delivery of RNA interference agents, such as antisense RNA, micro-RNA and siRNA. The formulations employ mixed micelles including amphipathic conjugates of the iRNA agents and amphipathic micelle- forming molecules with extended hydrophilic chains. Also disclosed are methods of using the pharmaceutical formulations to increase delivery of an iRNA agent to an intracellular target, and to decrease extracellular nuclease degradation of an i
...

Method of Preparing Adenosine-resistant Anti-tumor T Lymphocytes for Adoptive Immunotherapy

In the past, adoptive immunotherapy often failed because the transferred immune cells were inactive in vivo. This disclosure provides a method of producing immune cells that are highly active in vivo. The immune cells may be expanded in vitro in the presence of an adenosine receptor agonist or an antisense nucleic acid that downregulates expression of an adenosine receptor, for example. The immune cells may be tumor-infiltrating lymphocytes (TIL), cytotoxic T lymphocytes (CTL), natural killer (N
...

Imaging Neuroleptic Compounds

A method for identifying typical and atypical antipsychotics based on their ability to reduce neuronal/glial activity in specific brain regions upon dopaminergic neurotransmission is disclosed.
...

Collagen Fibrillar Construction

Methods and compositions are described for organizing collagen into fibrillar networks, e.g, short and long-range organization. Collagen produced by the disclosed methods can be used for tissue engineering.
...

Polycyclic Compounds for Use in Treating Ocular Neurodegenerative Diseases

Described herein are various compounds for treatment of ocular neurodegenerative diseases, including but not limited to glaucoma and diabetic retinopathy. The compounds described herein can act to attenuate and/or block calcium release from external neuronal environments as well as intracellular stores.
...

Methods of Treating Fungal Infections

Methods of identifying compounds that potentiate the activity of antifungal agents, potentiators identified by these methods, and methods of using potentiators are disclosed.
...

Methods of Targeting Cells for Diagnosis and Therapy

Methods of making bispecific binding complexes and nanopolymers coupled to detection and/or therapeutic agents are disclosed. Also disclosed are methods of using such bispecific binding complexes and nanopolymers for detecting and treating cells.
...

Self-assembling Micelle-like Nanoparticles for Systemic Gene Delivery.

Nanoparticles containing nucleic acid and suitable for use as in vivo delivery agents for nucleic acids are provided. The nanoparticles use a covalent conjugate of a polycation such as polyethylenimine and phospholipids. The final DNA- containing nanoparticle has a vesicular structure with a polyplex core surrounded by a mixed lipid/PEG- lipid monolayer envelope and offers simple preparation, high loading capacity, and in vivo stability. The nanoparticles have good in vivo stability and a prolon
...

Modulators of Nuclear Receptor Co-regulatory Protein Binding

Disclosed are novel compounds and compositions for inhibition of androgen and estrogen receptor signaling, methods for inhibiting androgen signaling, methods for inhibiting estrogen signaling, methods for inhibiting the interaction between a co-regulatory protein and an androgen or estrogen receptor, and methods for treating cancer.
...

Biomarkers for Diabetes, Obesity, and/or Hypertension

Methods of identifying subjects having, or at risk of developing, diabetes, obesity, and/or hypertension are disclosed, as well as methods of identifying biomarkers for diabetes, obesity, and/or hypertension, and biomarkers identified by such methods.
...

Methods and Compounds for Modulating Cannabinoid Activity

Disclosed are compounds and compositions that inhibit the action of monoacylglycerol lipase (MGL) and fatty acid amide hydrolase (FAAH), methods of inhibiting MGL and FAAH, methods of modulating cannabinoid receptors, and methods of treating various disorders related to the modulation of cannabinoid receptors.
...

T Cell Receptor-deficient T Cell Compositions

The invention is directed to modified T cells, methods of making and using isolated, modified T cells, and methods of using these isolated, modified T cells to address diseases and disorders. In one embodiment, this invention broadly relates to TCR-deficient T cells, isolated populations thereof, and compositions comprising the same. In another embodiment of the invention, these TCR-deficient T cells are designed to express a functional non-TCR receptor. The invention also pertains to methods of
...

Nutraceutical Composition and Methods for Preventing Or Treating Multiple Sclerosis

The present invention embraces nutraceutical compositions containing isolated Bacteroides fragilis capsular polysaccharide A for use in methods of preventing or treating multiple sclerosis.
...

Microrna-10 Antagonists and Microrna-10 Targets for Use in the Treatment of a Glioma

The present invention embraces microRNA-antagonists and activators of homeobox D10 protein; Zinc finger, MYND domain containing 11 protein; or RB1-inducible coiled-coil 1 protein for use in decreasing glial tumor cell proliferation and treating glioma.
...

Attenuated Uracil Auxotroph of an Apicomplexan and Use Thereof

Uracil auxotroph mutants of apicomplexans are provided which lack a functional carbamoyl phosphate synthase II (CPSII) enzyme. Also provided are T. gondii autoxtroph mutants which express exogenous antigens, and methods of protecting an animal against a T. gondii and non-T. gondii disease.
...

Compositions and methods for inhibiting the synthesis or expression of MMP-1

The present invention relates to the specific inhibition of matrix metalloproteinase 1 (MMP-1) using agents which inhibit the synthesis or expression of MMP-1. Such agents are useful for suppressing invasion or metastasis of a tumor cell and in the treatment, prevention and management of cancer.
...

Methods for diagnosing and treating neuroendocrine cancer

The present invention relates to a method for diagnosing neuroendocrine cancers via detecting the presence of N-methyl D-asparate-associated (NMDA) glutamate receptors type 1 and/or type 2. Methods for preventing and treating neuroendocrine cancers are also disclosed.
...

Cddo-compounds and Combination Therapies Thereof

CDDO-compounds in combination with other chemotherapeutic agents induce and potentiate cytotoxicity and apoptosis in cancer cell. One class of chemotherapeutic agents include retinoids. Cancer therapies based on these combination therapies are provided. Also provided are methods to treat graft versus host diseases using the CDDO compounds.
...

Compositions and Methods for Inhibiting the Interaction Between Cftr and Cal

The present invention features compositions and methods for increasing the cell surface expression of degradation-prone CFTR proteins and preventing or treating cystic fibrosis. The invention provides peptides and peptidomimetics that selectively inhibit the interaction between CAL and mutant CFTR proteins, thereby stabilizing the CFTR and facilitating transport of the same to the cell surface.
...

Selection of Cellulolytic Microbes with High Growth Rates

Methods for obtaining cellulolytic microbes with high growth rates are disclosed. For example, C. thermocellum colonies with growth rates higher than 0.17 hr " 1 have been obtained by the present methods. In realizing higher growth rates, better bioprocessing efficiency can be achieved resulting in increased economy.
...

Compositions and Methods for Diagnosis and Treatment of Pancreatic Ductal Cancer

The present invention includes compositions and methods for diagnosing and treating pancreatic cancer. These compositions and methods are based on the finding that 14-3-3s protein is secreted from pancreatic cancer cells and is therefore a specific biomarker protein.
...

Molecular Signatures for Diagnosing Scleroderma

The present invention features methods for classifying, determining severity, and predicting clinical endpoints of scleroderma based upon the expression of selected biomarker genes.
...

Prevention Or Treatment of Immune-relevant Disease By Modification of Microfloral Populations

The present invention is a method for preventing or treating an immune -relevant disease by modulating commensal microbiota populations via antibiotics, exogenous microbiota and/or probiotics.
...

Bioactive Molecules Produced By Probiotic Bacteria and Methods for Isolating and Using the Same

The present invention is a method for isolating bioactive molecules secreted by probiotic bacteria such as Lactobacillus rhamnosus, and methods for using such bioactive molecules to decrease replication of human immunodeficiency virus, expression of inflammatory cytokines and chemokines, expression of vasoendothelial growth factor, Erkl/Erk2 activation, and to inhibit HIV transmission.
...

Bis-carbazole DNA intercalating agents for antitumor therapy

The present invention is a DNA intercalating agent represented by the structure Y-Z-Y, wherein Y is an anti-tumor bis-carbazole and Z is a linear arrangement of multiple aromatic rings, containing at least two aromatic rings, or at least two alicyclic rings, said rings being linked in a 1,4 or 1,3 manner. Methods of inhibiting cancer cells and treating subjects having cancer with these agents are also provided.
...

Microrna Biomarkers for Human Breast and Lung Cancer

The present invention relates to novel molecular markers for diagnosis and classification of human breast cancer and lung cancer.
...

Method for Identifying an Agent That Inhibits Candida Albicans -mediated Host Cell Differentiation

The present invention features a method for identifying an agent that inhibits Candida albicans- mediated differentiation of keratinocytes. Agents identified by the screening assay of the invention find application in the prevention and treatment of candidiasis.
...

Caveolin 1-reporter Protein Knock-in Mouse

The present invention embraces a transgenic mouse whose genome harbors a nucleic acid molecule encoding caveolin-1 fused in-frame with a reporter. To control expression of the Cavl-reporter, the nucleic acid molecule further includes a selectable marker expression cassette flanked by recombinase target sites in such a manner that, upon exposure to recombinase, said selectable marker expression cassette is excised from said nucleic acid molecule.
...

Microrna-21 antagonists and its target PDCD4 for use in the treatment of a glioma

The present invention embraces microRNA-21 antagonists and activators of Programmed Cell Death 4 for use in decreasing glial tumor cell proliferation and treating glioma.
...

CELL CULTURE HYDROGEL WITH pH INDICATOR

The invention provides devices, compositions and methods for maintaining conditions in a cell culture and for measurement of conditions in the cell culture. In particular, the invention provides hydrogel materials, apparatus and methods for several non-invasive techniques of maintaining glucose and pH levels in cell cultures at near- optimal levels and the non-invasive measurement of pH levels in cell cultures.
...

Compositions and Methods for Treating Cancer with Dacarbazine Nanoemulsions

A uniform microfluidized nanoemulsion is disclosed containing an anti-cancer agent, such as dacarbazine. The microfluidized nanoemulsion improves the combination's cell membrane permeability by at least four-fold over conventional nanoemulsion compositions, which significantly increases the intracellular concentration of anti-cancer agents. As a nanoemulsion, dacarbazine has a greater anti-cancer efficacy than when applied as a free solution.
...

Synergetic Functionalized Spiral-in-tubular Bone Scaffolds

An integrated scaffold for bone tissue engineering has a tubular outer shell and a spiral scaffold made of a porous sheet. The spiral scaffold is formed such that the porous sheet defines a series of spiral coils with gaps of controlled width between the coils to provide an open geometry for enhanced cell growth. The spiral scaffold resides within the bore of the shell and is integrated with the shell to fix the geometry of the spiral scaffold. Nanofibers may be deposited on the porous sheet to
...

Structural/biological Implant System

A structural/biological implant and method of use. The implant being utilized as a single or multiple staged system that is designed to encourage new alveolar bone growth with or without the need to obtain autologous bone. The implant has an apical portion that is fastened into existing bone, with the remainder of the implant left outside of existing bone. The exposed portion of the implant may have an external shape or configuration with a variety of attached and/or integrally formed mechanical
...

Biosensor for Continuous Monitoring of Metabolites and Proteins and Methods of Manufacture Thereof

A biosensor comprises a substrate; a reference electrode; a working electrode; a counter electrode; and a plurality of permeability adjusting spacers. The reference electrode, the working electrode and the plurality of permeability adjusting spacers are all being disposed to be substantially parallel to each other to create a plurality of enzyme containing porous sections. The enzyme containing porous sections contain an enzyme; where the enzyme is operative to react with a metabolite to determi
...

Methods of Inhibiting Vascular Permeability and Apoptosis

Agonists of vascular endothelial sphingosine-1-phosphate receptors are described. Compounds such as FTY720 can be phosphorylated by sphingosine kinase-2 into the phosphorylated forms which serve as sphingosine-1-phosphate receptor agonists. The vascular endothelial sphingosine-1-phosphate receptor agonists are employed in methods of treating a mammal for vascular permeability disorders and unwanted vascular endothelial cell apoptosis, and for the growth of new blood vessels. The sphingosine-1-ph
...

Differentiation of Human Embryonic and Induced Pluripotent Stem Cells

The invention relates to culture systems, methods, and conditions that allow pluripotent undifferentiated hESCs or iPSCs to progressively and uniformly differentiate into cells of the chondrogenic lineage.
...

Fluorinated Lipids and Methods of Use

The invention provides methods and compounds for delivering agents to cells. The compounds can include a fluorinated lipid, a linker, and an agent for delivery to a cell.
...

Polymeric Microspheres

The invention features core-shell microsphere compositions, hollow polymeric microspheres, and methods for making the microspheres. The microspheres are characterized as having a polymeric shell with consistent shell thickness.
...

Methods and Compositions for Modulating Membrane Potential to Influence Cell Behavior

The present invention provides methods for controlling proliferation, differentiation, and/or migration of cells by modulating membrane potential through an endogenously expressed channel protein. The present invention also provides methods for identifying candidate instructor cells, as well as therapeutic agents that are useful for modulating (e.g., promote or inhibit) proliferation and differentiation, as well as promoting regeneration of a desired tissue type.
...

Measurement of Biological Targets

An integrated imaging system, e.g., a microsystem, for detecting activity of a biological target, for example, in response to a stimulus, includes an array of pixels arranged on a substrate, each pixel including a photodetector disposed to be in optical communication with a biological target on a surface of the substrate; and at least first and second electrodes arranged in electrical communication with the biological target.
...

Smad Proteins Control Drosha-mediated Mirna Maturation

The invention, in some aspects, relates to compositions and methods useful for modulating expression of miRNAs that are regulated by the TGF-ss/BMP signaling pathway. In some aspects, the invention relates, to oligonucleotides comprising a CAGRN-motif that modulate expression of miRNAs that are regulated by TGF-ss/BMP signaling pathway. The invention, in some aspects, relates to composition and methods useful for inhibiting microRNA processing. In some aspects, the invention relates to compositi
...

Anti-apoptotic Agents and Uses Thereof

The invention generally relates to the use of parasite-derived neurotrophic factor (PDNF), or a fragment of PDNF, to reduce cell apoptosis. The PDNF or PDNF fragment is provided in the cytoplasm of a cell so that it can bind to Akt kinase and induce Akt kinase activation.
...

Silk Biomaterial and Method of Use Thereof

The present invention provides an all-aqueous process and composition for production of silk biomaterials, e.g., fibers, films, foams and mats. In the process, at least one biocompatible polymer, such as poly(ethylene oxide) (PEO) (a well-documented biocompatible material), was blended with the silk protein prior to processing e.g., electrospinning. We discovered that this step avoids problems associated with conformational transitions of fibroin during solubilization and reprocessing from aqueo
...

Cationic Contrast Agents and Methods of Use Thereof

The present invention provides compounds useful as contrast agents, such as for the CT imaging of cartilage tissue. The contrast agents are generally iodinated organic molecules that are positively charged under physiological environments. Also provided are compositions containing contrast agents and methods of using the agents, including, for example, the monitoring of glycosaminoglycan content in cartilage tissue. The invention provides non-invasive analytical techniques for the diagnosis of o
...

Engineered Enzymatically Active Bacteriophage and Methods for Dispersing Biofilms

The present invention is directed to engineered enzymatically active bacteriophages that are both capable of killing the bacteria by lysis and dispersing the bacterial biofilm because they have been also engineered to express biofilm-degrading enzymes, particularly dispersin B (DspB), an enzyme that hydrolyzes beta-1,6-N-acetyl-D-glucosamine, a crucial adhesion molecule needed for biofilm formation and integrity in Staphylococcus and E. coli, including E. coli K-12, as well as clinical isolates.
...

Nanochannel-based Sensor System for Use in Detecting Chemical Or Biological Species

A sensor system for detecting a chemical or biological species includes a sensing element and a bias and measurement circuit. The sensing element includes nanochannels, each having an outer surface functionalized to chemically interact with the species to create a corresponding surface potential, and each having a sufficiently small cross section to exhibit a shift of a differential conductance characteristic into a negative bias operating region by a shift amount dependent on the surface potent
...

Alien Sequences

The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.
...

Method for Bacterial Lysis

The present invention is directed to a microfluidic device for lysis of cells, such as bacteria and microorganisms. In particular, the present invention relates to microfluidic devices and methods of manufacture of such microfluidic devices comprising a substrate with at least one channel packed with a polymer monolith embedded with carbon particles. The microfluidic devices and methods of the present invention are useful for cell lysis of cells within a biological sample, such as a untreated bi
...

High Magnification Spectral Reflectance Biosensing with Discrete Light Sources

A spectral reflectance imaging device for detecting biomolecular targets includes an illumination source that illuminates a substrate with a plurality of separate wavelengths of incoherent light. The substrate includes an oxide layer and a binding agent to selectively bind biomolecular targets to the substrate. An imaging device captures the light reflected from or transmitted through the substrate and an image processing system detects the biomolecular targets a function of the change in reflec
...

Bacteriophages Expressing Antimicrobial Peptides and Uses Thereof

The present invention is generally related to engineered bacteriophages expressing antimicrobial peptides or lytic enymes or fragments thereof for targeting a broad spectum of bacterial hosts, and for the long-term suppression of bacterial phage resistance for reducing bacterial infections. In some embodiments, bacteriophages express antimicrobial peptides or antimicrobial polypeptides (e.g. phage lytic enzymes) which are secreted from the host bacteria, or alternatively released upon lysis of t
...

Nucleic Acid Supported Protein Complementation

The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive polypeptide fragment are brought in close proximity during hybridization to a target nucleic acid, they re-associate into an active, detectable protein.
...

Isolation of Rna

Disclosed herein are methods for purification of RNA from a sample. The method involves preparing the sample in a solution of lysis buffer, depositing it into a first end of a lysis straw such that the sample solution flows through the matrix of the lysis straw and is eluted from its opposite end, and depositing the eluted material into a first end of a solid phase extraction (SPE) straw, such that the deposited solution flows through the matnx of the SPE straw towards its opposite end, and elut
...

Sequence Preserved Dna Conversion

Described herein are inexpensive high throughput methods to convert a target single stranded DNA (ssDNA) such that each nucleotide (or base) adenine (A), thymine (T), guanine (G) and cytosine (C) is converted to a pre-determined oligonucleotide code, with the sequential order preserved in the converted ssDNA, or RNA. The method does not require the use of DNA polymerases during the cycles and involves the use of an oligonucleotide probe library with repeated cycles of ligation and cleavage. At e
...

Nucleic Acid Cloning

The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product mole
...

Bacteriophages Expressing Amyloid Peptides and Uses Thereof

The present invention generally relates to engineered bacteriophages which express amyloid peptides for the modulation (e.g. increase or decrease) of protein aggregates and amyloid formation. In some embodiments, the engineered bacteriophages express anti-amyloid peptides for inhibiting protein aggregation and amyloid formation, which can be useful in the treatment and prevention of and bacterial infections and biofilms. In some embodiments, the engineered bacteriophages express amyloid peptides
...

Bioreactive Agents

This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled
...

Antimicrobial Ionic Liquids

Certain aspects of the present invention relate to compositions, formulations, and use of ionic liquids as antimicrobial agent (e.g. as antibacterial and/or antifungal agents). In certain embodiments, the ionic liquids can be pared with one or more singly charge species or with one or more multi-charged species.
...

Melanoma Biomarker and Methods of Uses

The present invention relates to the use of TROY, also called tumor necrosis factor receptor superfamily, member 19 (TNFRSF19) also called toxicity and JNK inducer (TAJ), in diagnosis and therapy of non-epithelial cancers, such as melanoma. Accordingly, the invention provides in vitro and in vivo diagnostic and/or prognostic methods for cancers, other than epithelial cancers, preferably melanoma, comprising analyzing TROY expression in a biological sample from an individual or in an individual,
...

Label-free Sensing of Pna-dna Complexes Using Nanopores

Embodiments disclosed herein relate to a method of detecting specific DNA sequences and the application of this method in the detection of pathogens, viruses, drug-resistant pathogens, genomic variations associated with disease/disorder susceptibility etc. based on specific signature sequences unique to the pathogens, viruses, drug-resistant pathogens or genomic variations. The method can also be used to distinguish a pool of same-sized dsDNA on the basis of sequence differences. The method uses
...

Chromosome Therapy

The present invention features compositions and methods for introducing, into cells, nucleic acids whose expression results in chromosomal silencing. The nucleic acids are targeted to specific chromosomal regions where they subsequently reduce the expression of deleterious genes, or cause the death of deleterious cells. Where the nucleic acid sequence is a silencing sequence, it may encode an Xist RNA or other non-coding, silencing RNA. Accordingly, the present invention features, inter alia, nu
...

Nucleic Acid Silencing Agent-protein Conjugates and Use Thereof for Treating Hcv-related Disorders

A method of using a complex or a molecule comprising a targeting moiety to deliver a second molecule effectively to HCV or to a cell being infected or having the potential to be infected by HCV, resulting in the prevention, immunization, inhibition or cure of HCV infection, or other beneficial effects related to HCV infection
...

Tryptophan As a Functional Replacement for Adp-ribose-arginine in Recombinant Proteins

A method is disclosed for producing a polypeptide with a modified activity or stability, by replacing an arginine residue capable of being ADP-ribosylated with a tryptophan or a phenylalanine. In one embodiment, compositions are provided that include polypeptides, such as alpha defensin, with arginine-to-tryptophan or arginine-to-phenylalanine substitutions, where the arginine residue is capable of being ADP-ribosylated. In another embodiment, methods are disclosed for modifying an immune respon
...

Stabilized Liposome Compositions and Related Methods of Use

Liposome compositions and related methods of preparation which can comprise one or a plurality of biopolymer components.
...

Measuring Tissue Oxygenation

Methods and systems for calculating tissue oxygenation, e.g., oxygen saturation, in a target tissue are disclosed. In some embodiments, the methods include: (a) directing incident radiation to a target tissue and determining reflectance spectra of the target tissue by measuring intensities of reflected radiation from the target tissue at a plurality of radiation wavelengths; (b) correcting the measured intensities of the reflectance spectra to reduce contributions thereto from skin and fat layer
...

Toll-like Receptor-based Biosensors

Compositions and methods for detecting Toll-like receptor binding to ligands and test compounds are disclosed herein.
...

Interleukin 6 and Tumor Necrosis Factor Alpha As Biomarkers of Jnk Inhibition

Biomarkers for JNK inhibition are described that can be used for monitoring effectiveness of JNK inhibitors and monitoring treatment with JNK inhibitors.
...

Enhanced Transport Using Membrane Disruptive Agents

Compositions and methods for transport or release of therapeutic and diagnostic agents or metabolites or other analytes from cells, compartments within cells, or through cell layers or barriers are described. The compositions include a membrane barrier transport enhancing agent and are usually administered in combination with an enhancer and/or exposure to stimuli to effect disruption or altered permeability, transport or release. In a preferred embodiment, the compositions include compounds whi
...

Methods and Compositions to Reduce Oxidative Stress

The invention relates to therapeutic applications for compositions that reduce the level of oxidative stress on cells in vivo or in vitro. The invention describes methods for improving the therapeutic properties of stem cells. The invention also provides combination therapies that are useful to balance the oxidative microenvironment of cells in vivo or in vitro.
...

Toll-Like Receptor 9 Modulators

TLR9 is localized to endoplasmic reticulum and upon stimulation with a TLR9 ligand, is transported to a tubular lysosomal compartment as is CpG-DNA. Furthermore, it is shown that TLR9 and CpG-DNA directly bind. It was also found that the MyD88 translocates in response to activation of TLR9-mediated signaling. Methods of identifying compounds that affect translocation and activity of TLR9 and MyD88 are described.
...

Methods for Microbial Production of Terpenoids

The invention relates to recombinant expression of terpenoid synthase enzymes and geranylgeranyl diphosphate synthase (GGPPS) enzymes in cells and the production of diterpenoids.
...

Methods and compositions for enhancing the efficacy and specificity of RNAi

A method of enhancing the efficacy of an siRNA duplex, or promoting entry of an antisense strand of an siRNA duplex into a RISC complex, the siRNA duplex comprising the antisense strand and a complementary strand, the strands each having 5' and 3' ends, the method comprising enhancing the asymmetry of the siRNA duplex by lessening the base pair strength between the 5' end of the antisense strand (AS 5') and the 3' end of the sense strand (S 3') relative to the base pair strength between the 3' e
...

Multipurpose Microfluidic Device for Mimicking a Microenvironment Within a Tumor

The present application relates generally to a novel microfluidic device for the in it propagation of neoplastic cellagregates under conditions that mimic the physiological microenvironment found in tumors. The invention also describes methods of screening for therapeutic test agents and protocols that target proliferating and quiescent neoplastic cells within tumors.
...

Complement Factor H-based Assays for Serum Bactericidal Activity Against Neisseria Meningitidis

Assays for detection of bactericidal anti-Neisserial antibodies using a factor H polypeptide having a human amino acid sequence that mediates binding to Neisserial factor H binding protein (fhBp) are provided, as well as non-human animal models of Neisserial infection.
...

Staphylococcus Detection Assays

Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of various Staphylococcus species and types. In particular, provided herein are assays that allow the detection of MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, and MRSA community strain by detecting the presence of absence of one or more target sequences selected from a list of target genes and sequences that include, but are not limited to: mecA, femA-SA, femA-SE, lukF-PV, lu
...

Nucleic Acid Processing Methods, Kits and Devices

Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single co
...

Primers, Probes and Methods for Nucleic Acid Amplification

Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-P
...

Reagents and Methods for Pcr

Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least 32 DEG C, and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified olgonucleotide being capable of binding to t
...

Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification

An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
...

Methods of Using Mir-199a As a Marker and Sequences of Mir-199a As a Therapeutic for Cancer

The invention relates to methods of measuring the level of expression of differentially expressed microRNAs (miRNAs), such as for example hsa-mir-199a, in a cancer cell as an indicator of the level of chemoresistance of the cancer cell. The invention further relates to methods of administering a nucleic acid, having a sequence based upon the sequence of a differentially expressed miRNA, such as for example hsa-mir-199a, to a cancer cell to diminish the chemoresistance of the cancer cell.
...

Use of the Combination of Phy906 and a Tyrosine Kinase Inhibitor As a Cancer Treatment Regimen

This invention provides herbal compositions useful for increasing the therapeutic index of chemotherapeutic compounds. This invention also provides methods useful for improving the quality of life of an individual undergoing chemotherapy. Furthermore, this invention improves the treatment of cancer by administering the herbal composition PHY906 in combination with one or more chemotherapeutic compounds to a mammal undergoing such chemotherapy.
...

Rock Inhibitors and Uses Thereof

The subject invention concerns compositions and methods for blocking cancer cell growth or proliferation and/or inducing cancer cell death. Compositions of the present invention are compounds that inhibit Rho-protein associated kinase function. Compounds of the invention include piperazinyl pyridines, piperazinylmethyl pyridines, piperazinyl ureas and carbamates, piperazinyl pyridines and quinoilines (including isoquinliones) as well as piperazinyl (including piperazinylmethyl) pyridines and qui
...

Methods and Compositions Related to Riboswitches That Control Alternative Splicing.

Disclosed are methods and compositions related to riboswitches that control alternative splicing.
...

Aav-mediated Delivery of Dna to Cells of Nervous System

PROBLEM TO BE SOLVED: To provide a method of delivering a DNA exogenous to both an adeno-associated virus (AAV) and a target cell of the mammalian nervous system. ; SOLUTION: The method includes the step of supplying AAV-derived vectors modified to comprise the DNA exogenous to both AAV and the target cell of the mammalian nervous system; and the step of transducing the vector into the cell. The exogenous DNA preferably contains a gene encoding a gene product useful for treatment of nervous syst
...

A Snp Marker of Breast and Ovarian Cancer Risk

The invention provides methods for predicting an increased risk or probability of developing breast or ovarian cancer in a patient based upon the patient's KRAS-Variant and BRCA status.
...

Tissue Engineering of Lung

The present invention relates to compositions comprising a decellularized tissue. The present invention also provides an engineered three dimensional lung tissue exhibiting characteristics of a natural lung tissue. The engineered tissue is useful for the study of lung developmental biology and pathology as well as drug discovery.
...

Novel Markers for Detection of Complications Resulting from in Utero Encounters

Described herein are biomarkers, such as protein biomarkers, which are diagnostic of and predictive for complications that result from an in utero encounter, such as an infection by the fetus, that can lead to premature birth (PTB). The biomarkers can be used to identify fetuses and newborns at risk for complications of PTB, such as (Early Onset Neonatal Sepsis) EONS, intra-ventricular hemorrhage (IVH) and other poor outcomes.
...

Compositions and Methods of Use of an Oncolytic Vesicular Stomatitis Virus

Oncolytic VSV viruses have been developed as a strategy for combating cancer. The present invention includes mutant VSV that have one or more mutations in the nucleic acid sequence encoding the viral genome that increase the oncolytic potential of the virus. Pharmaceutical compositions including oncolytic virus disclosed herein are also provided. Pharmaceutical compositions containing virus and one or more excipients may be for systemic or local administration. Methods of administering an effect
...

Detection, Isolation and Uses of Renalase (monoamine Oxidase C)

The present invention provides for the identification, isolation and uses of mammalian Monoamine Oxidase C (MAO-C), also known as renalase.
...

Identification of cancer protein biomarkers using proteomic techniques

The claimed invention describes methods to diagnose or aid in the diagnosis of cancer. The claimed methods are based on the identification of biomarkers which are particularly well suited to discriminate between cancer subjects and healthy subjects. These biomarkers were identified using a unique and novel screening method described herein. The biomarkers identified herein can also be used in the prognosis and monitoring of cancer. The invention comprises the use of leptin, prolactin, OPN and IG
...

Semaphorin 7A Plays a Critical Role in TGF-Beta1-Induced Pulmonary Fibrosis and Alveolar Destruction

The present invention provides methods of treating a subject diagnosed with, or at risk for developing, pathogenic fibrosis, particularly pulmonary fibrosis. The method of the invention comprises administering to the subject a compound or composition which inhibits semaphorin (SEMA) 7A, SEMA 7A receptors, or downstream effectors. A SEMA 7A inhibitor comprises an antibody, a soluble SEMA 7A receptor, an siRNA, a ribozyme, an antisense, an aptamer, a peptidomimetic, a small molecule, a soluble rec
...

Inhibitors of Receptor Tyrosine Kinases and Methods of Use Thereof.

The present invention provides moieties that bind to an Ig-like domain, e.g., D4 or D5, of a human receptor tyrosine kinase, e.g., the human Kit RTK or the PDGFR RTK, or the D7 domain of a type V receptor tyrosine kinase wherein the moieties lock the ectodomain of the receptor tyrosine kinase in an inactive state thereby antagonizing the activity of the receptor tyrosine kinase.
...

Methods and Compositions Related to Riboswitches That Control Alternative Splicing and Rna Processing.

Disclosed are methods and compositions related to riboswitches that control alternative splicing.
...

Riboswitches and Methods and Compositions for Use of and with Riboswitches.

Riboswitches are targets for antibiotics and other small molecule therapies. Riboswitches and portions thereof can be used to regulate the expression or function of RNA molecules and other elements and molecules. Riboswitches and portions thereof can be used in a variety of other methods to, for example, identify or detect compounds. Compounds can be used to stimulate, active, inhibit and/or inactivate the riboswitch. Riboswitches and portions thereof, both alone and in combination with other nu
...

Methylation Biomarkers and Methods of Use

Disclosed are methods and compositions of assessing one or more statuses of a subject. Also disclosed are methods and compositions of identifying status biomarkers associated with a status of a subject. Also disclosed are sets of one or more status biomarkers. Also disclosed are methods and compositions of producing status biomarker capture probes.
...

Identification of Cancer Protein Biomarker Using Proteome Technique

PROBLEM TO BE SOLVED: To provide a testing method capable of being used in order to diagnose the cancer of an ovary in an initial stage or an advanced stage. ; SOLUTION: There are disclosed a method for diagnosing the cancer or assisting the diagnosis of the cancer. The method is based on the identification of a biomarker especially fitted to discrimination between a cancerous subject and a healthy subject. The biomarker is identified using a novel original screening method. The identified bioma
...

Instruments, Methods and Systems for Harvesting and Implanting Cartilage Materials

The present disclosure provides instruments and systems for accessing and removing hyaline cartilage from desired donor sites. The present disclosure also provides instruments/systems for implantation of hyaline cartilage grafts, e.g., to fill osteochondral defects. The apparatus/systems may be used in connection with mapping techniques and systems. Thus, in exemplary embodiments of the present disclosure, a clinician may be guided in his use of the disclosed apparatus/systems by articular joint
...

Single Nucleotide Polymorphisms in Brca1 and Cancer Risk

The invention provides methods for identifying mutations, such as single nucleotide polymorphisms (SNPs), within breast and ovarian cancer associated genes that modify the binding efficacy of micro RNAs (miRNAs). In a preferred embodiment, methods of the invention identify a SNP that decreases expression of the BRCA1 gene by increasing or decreasing the binding efficacy of at least one miRNA. Alteration of miRNA binding to BRCA1 by the introduction of SNPs within miRNA binding sites modulates or
...

Jnk3 Modulator and Method for Using the Same

PROBLEM TO BE SOLVED: To provide a method for screening a molecule and compound reducing the expression or activity of the c-Jun NH<SB>2</SB>terminal kinase (JNK) group of a MAP kinase. ; SOLUTION: The method for identifying a JNK3 expression-modulating compound includes: incubating the expressing cells of the JNK3 protein in the presence of the compound; then measuring the JNK3 expression and comparing the expressed amount with that of control cells under the non existence of the compound. The
...

Compositions and Methods for Inhibiting Inflammation from and Rejection of Biomaterials and Other Methods

The present invention relates, inter alia, to methods and compositions for regulating the host response to biomaterials, including inhibiting inflammation from and rejection of biomaterials using salicylate compounds and/or TLR7/TLR9 antagonists as described herein.
...

Methods of Regulating Renalase (monoamine Oxidase C)

The present invention provides methods of using Monoamine Oxidase C (MAO-C), also known as renalase, as a therapeutic protein in its active and inactive forms. Administering inactive renalase protein to individuals with lower renalase levels can be used to provide them with an adequate pool of the protein that can be activated by the body as needed. Active renalase protein can be administered to individuals needing an immediate reduction of catecholamine levels. An inhibitor of renalase may be u
...

Regulation of oncogenes by microRNAs

Naturally occurring miRNAs that regulate human oncogenes and methods of use thereof are described. Suitable nucleic acids for use in the methods and compositions described herein include, but are not limited to, pri-miRNA, pre-miRNA, mature miRNA or fragments of variants thereof that retain the biological activity of the mature miRNA and DNA encoding a pri-miRNA, pre-miRNA, mature miRNA, fragments or variants thereof, or regulatory elements of the miRNA. The compositions containing nucleic acids
...

Immunization to Reduce Neurotoxicity During Treatment with Cytolytic Viruses

Methods for protecting the brain and other tissues from virus- associated toxicity are provided. Patients are treated with a first immunizing virus prior to administration of a second, therapeutic cytolytic virus. The immunizing virus is preferably administered peripherally, and initiates an adaptive immune response that is sufficient to reduce or prevent the cytovirulence and inflammation caused by the therapeutic virus. The immunizing virus is administered one or more times, preferably at leas
...

Toll-like Receptor 4 Deficiency and Downstream Effectors Cause Pulmonary Emphysema

The present invention provides compositions and methods for the detection, treatment, and prevention of emphysema/COPD. Compositions of the present invention comprise TLR4 activators, Nox3 inhibitors, and Cathepsin E inhibitors useful in the treatment or prevention of emphysema/COPD. Cathepsin E is a downstream effector of TLR4, wherein when cathepsin E is overexpressed in lung of an individual, the individual is at higher risk of developing emphysema/COPD. Cathepsin E is further identified as a
...

Targeting TGF-beta as a Therapy for Alzheimer's Disease

The invention includes compositions and methods for enhancing peripheral macrophage A[beta] phagocytosis activity. The invention includes inhibiting a component of TGF-[beta] signaling pathway in peripheral macrophages to promote central nervous system infiltration and beneficial cerebral A[beta] clearance. Inhibition of TGF-[beta] signaling in peripheral macrophages represents an advantageous anti-amyloid therapeutic approach for Alzheimer's disease.
...

Systems, Devices and Methods for Cartilage and Bone Grafting

Disclosed are systems, methods, devices and products to identify suitable donor sites for harvesting bone-cartilage grafts and to implant such bone-cartilage grafts. In some embodiments, a method includes providing a computer having access to a donor database, the donor database comprising information on each of a plurality of donor joint sites of the body, receiving first data relating to a defect of a joint of a patient, the defect comprising an area of bone, a portion of which includes at lea
...

Small Molecule Inhibitors of Autotaxin and Methods of Use

Autotaxin (ATX) is a prometastatic enzyme initially isolated from the conditioned media of human melanoma cells that stimulates a myriad of biological activities including angiogenesis and the promotion of cell growth, survival, and differentiation through the production of lysophosphatidic acid (LPA). ATX increases the aggressiveness and invasiveness of transformed cells, and ATX levels directly correlate with tumor stage and grade in several human malignancies. To study the role of ATX in the
...

Method for the Determination of Phospholipids in Amniotic Fluid Samples

The present invention refers to a fast method for the determination of phospholipids in amniotic fluid samples by HPTLC (high performance thin layer chromatography). This innovative method presents high sensitivity, reproducibility and resolution. It has also the advantage of being of easy laboratorial interpretation, allowing the elimination of false results due to the contamination of the sample with blood or meconium.
...

Braided Corrugated Textile Vascular Prosthesis and Process of Producing Same

The present invention relates to a braided corrugated vascular prosthesis with a textile structure consisting of at least two types of biocompatible fibres, of which one is absorbable by the human body, and to the process of producing same using conventional braiding equipment, by combining previously braided biocompatible threads so as to form strings, on a mandrel (4) and a (3), to ensure the necessary diameter and corrugation. The vascular prosthesis is homogeneously impregnated with a substa
...

Compositions Comprising Antioxidant and Mitoprotective Flavonoids with Neuroprotective Properties

The present invention discloses compositions comprising phenolic compounds present in plant extracts with therapeutic activity against neuronal excitotoxicity, oxidative stress and mitochondrial dysfunction. When used individually or in a formula composition quercetin, kaempferol and biapigenin significantly reduced neuronal death caused by excitotoxic insults or to exposure to amyloid-beta peptide. Moreover, it is also disclosed that biapigenin interferes with the ANT/mitochondrial transition p
...

EstÉrase Thermophile De Thermus Thermophilus

The invention relates to a thermophilic protein with esterase activity. Said protein, of the Thermus thermophilus organism, is expressed in recombinant yeasts and bacteria, in particular E.coli, K.lactis and S.cerevisiae. The esterase is expressed in fusion protein form with a signal sequence that enables secretion to the culture medium and, in addition, is operably linked to an inducible promoter. The recombinant strains are advantageous in that: (i) the cultivation thereof is much easier than
...

Biphasic calcium phosphate and method for obtaining same from fish bones

The present invention relates to a biphasic calcium phosphate comprising between 5 and 40% by weight of beta-tricalcium phosphate; between 0.1 and 0.6% by weight of Mg; between 0.2 and 0.8% by weight of Na; between 0.1 and 0.5% by weight of Sr; between 0.01 and 0.1% by weight of K; the remaining part being hydroxylapatite, to processes for obtaining same as well as to its use as a biocompatible material, particularly as a bone replacement material.
...

Use of a Casein-derived Peptide and Compositions Thereof As Antihypertensive

Use of a casein-derived peptide with antioxidant and ACE-inhibitory activity in vitro and antihypertensive activity in mammals, and compositions thereof. This peptide may be use as active ingredient in pharmaceutical, cosmetic and/or dermatological compositions, dietary supplements or as food ingredient. Also relates with composition comprising this peptide in specific concentrations, and fermented compositions, including fermented dairy product, that have enhanced stability under various storag
...

Trangenic mice used as model for human pathologies originating from stem cells

Transgenic non-human mammals that reproduce the human pathology that is of stem cell origin, for example, chromosomal anomalies associated with chronic myeloid leukaemia, B-cell acute lymphoblastic leukaemia, T-cell acute lymphoblastic leukaemia, or with the migration of haematopoietic or embryonic stem calls, using as a strategy the expression of the genes involved in said pathology in human beings by means of a promoter that directs the expression of a transgene in Sca-1<+> cells. Said transge
...

Cell-culture Flow Bioreactor

Cell-culture flow bioreactor that comprises an incubator (1), the receptacle (14) of which, to which access is gained via a hermetically sealed door (13), is defined by thermally insulating walls, and which is provided with a system for pumping fluid that circulates via the interior of a plurality of culture circuits where the cell-seeded scaffolds are housed, which comprises: - at least one motor (6, 7) for actuating at least one impulsion means (8, 20) arranged in the exterior of the incubator
...

Liposomes Covered with the Extracellular Domain of the Apo2l/trail Protein

The invention relates to the field of biotechnology and medicine. The invention specifically relates to a compound comprising a liposome covered or decorated at least with the extracellular domain of the APO2L/TRAIL protein, and to the use thereof for developing a medicament preferably for the treatment of a cancer or inflammatory or autoimmune diseases, such as rheumatoid arthritis.
...

Smart Packaging for Detecting Microorganisms

The present invention relates to novel smart packaging, designed using a novel material comprising a partially polar adsorbent solid base impregnated with a solution of vanillin, which allows the growth of microorganisms in different types of products to be detected visually without having to be in direct contact with the microorganism or with the medium containing same.
...

Vectors and Uses of the Mboumar Transposon

The invention relates to the recombinant production of a naturally active transposase of Messor bouvieri, transposon Mboumar-derived genetic constructions necessary for transgenesis and mutagenesis, and the uses thereof.
...

Novel Amperometric Biosensor, Production Method Therefor and Use of Same

The invention relates to a novel amperometric biosensor, the production method therefor and use of same. The invention is based on a combination of the laccase enzyme and sonogel-carbon materials. The invention can be used to take in situ measurements of analytes of interest in agri-food samples during the production, packaging and/or storage processes. In addition, given the high selectivity owing to the presence of biological substances, the same design can be adapted for a wide range of sampl
...

Method for Enhancing the Effectiveness of Bacterial Strains

A method for enhancing the pathogenicity of bacterial strains of the genus Bacillus, which comprises exposing the strain to temperatures below ambient temperature or other situations of stress, a composition of bacterial strains obtained by said method and the use of said composition in the biological control of the Ceratitis capitata pest are described.
...

Single-labelling Agents Based on Vinyl Sulphone

The invention relates to labelling agents containing a compound with a labelled molecule and a vinyl sulphone group. The invention also relates to the compounds, the method for obtaining same and the uses thereof in the marking of biomolecules and, more specifically, proteins.
...

Compound of Silica-vinylsulphone, Synthesis and Uses of the Same

Compound which comprises a medium of silica functionalized with vinylsulphone groups. In addition, the invention describes the method for production thereof and uses thereof as an immobilizer of biomolecules, and more specifically of enzymes such as invertase, lactase, peroxidase or thioredoxin h1 and other biomolecules such as glutathione, nitrosoglutathione or biotin.
...

Porous Biotin Carrier, and Associated Production Methods and Uses

The invention relates to a compound comprising biotin joined to a porous carrier by means of a linker containing the group 1, 2, 3-triazole. The invention also relates to the method for obtaining said compound and to the uses thereof for purifying avidin and biotinilated proteins, and for the fluorescent marking of avidin.
...

Strain Cect 7462 of Bacillus Pumilus and a Method for Increasing the Entomopathogenic Effects Thereof

The invention relates to a novel bacterial strain of Bacillus pumilus (CECT 7462 or B. pumilus TCC). Said strain can be treated with exposure to low temperatures in order to increase the entomopathogenic effects thereof. The invention also relates to compositions that include the original strain or the strain treated using the method of the invention and/or the crystalline structures thereof and to the uses of same for biological pest control and for the production of bioinsecticides. The invent
...

Antibodies for Treating Cancer

The invention relates to the use of an antibody or a fragment thereof, specific against substance P, for the manufacture of a medicament for therapeutic treatment of a cancer expressing NK1 receptor and/or a ErbB receptor family member (i.e. the epidermal growth factor receptors family), by direct administration to a mammal, including a human. The administration of an antibody against substance P to a panel of breast cancer cell lines, inhibits HER2 activation and expression, causing a downregul
...

GENOME SEQUENCES OF PRIMERS AND a PROBE FOR THE QUANTIFICATION OF PORCINE ADENOVIRUSES (PAdV)

The invention relates to a sensitive test for the detection of porcine adenoviruses in environmental samples, water and food. Specifically, the invention relates to a quantitative tool intended for the analysis of porcine adenoviruses as indicators of the presence of porcine contamination. The invention further relates to a kit comprising two sequences of primers and a probe sequence capable of detecting and quantifying the aforementioned virus by means of PCR. The test has been proven to have g
...

Cis Bicyclic Complexes of Platinum (ii) Having Anticancer Activity

The invention relates to compounds having formula (I), wherein: the broken line represents a single or double bond; X is N, O, S, or -(CH2)n-; n is an integer between 1 and 3; R1 is a radical selected independently from among (C1-C4) alkoxyl, phenoxyl, phenyl-(CH2)r-O; R2 and R'2 represent a radical selected independently from among hydrogen, hydroxyl, (C1-C4)-alkoxyl, phenoxyl, phenyl-(CH2)r-O, and O-Gp; r is an integer between 1 and 4; Gp is an alcohol protective group, whereby the radicals wi
...

Novel Chemotherapeutic Agents for Cancer Treatment

The invention relates to compounds having formula (15), shown below, in which R1 is H or I, R2 is a radical selected from (C1-C16)-alkyl, phenyl and benzyl, R3 is a (C2-C4)-alkyl group and the configuration of C in substituent (R2O)(R3COO)CH is R or S, which compounds have been shown to be active against a plurality of human cancerous cell lines, in particular against the cell lines of melanoma, leukemia, non-small cell lung cancer, renal cancer, ovarian cancer, colon cancer, CNS cancer, breast
...

Novel Chemotherapeutic Agents for Treating Cancer

The compounds of formula (I) where: R1, R2, R3 and R4 are independently H, or a radical selected from hydroxyl; -OR; and -OCOR; where R is a radical selected from (C1-C4)-alkyl, phenyl, -(CH2)n-phenyl with n =1 - 4, and a radical derived from the above-mentioned with benzene rings, by means of a mono-, di- or tri-substitution in the benzene rings thereof; the substitutes being a radical selected from halogen, (C1-C4)-alkyl, (C1-C4) alkoxyl and trifluoromethyl, R4 also includes halogen, and R1, R
...

Cranial Implant

An Implant, such as a cranial implant (3), for attachment to a defect (2) in a bone structure (1) having an edge (4) is described. The implant comprises a direction of extension and a rim (5) extending substantially perpendicular to the direction of extension of the implant. The rim is configured to be attached to the edge of the bone structure and comprises at least one mounting portion (6) configured to accommodate a fastener (7) such that at least a portion (7B) of the fastener is extendible
...

Use of Glycosaminoglycan for Restoring Glycocalyx

The invention is directed to the use of glycosaminoglycans for restoring glycocalyx functions or preventing glycocalyx damage. The effectiveness of glycosaminoglycans is directed in all pathologies where glycocalyx damage or this function may be etiologically cause of many pathologies such as: vascular disease, septic states, atherosclerosis syndrome, inflammation states and disease related to ischemia, peritoneal impairment, myocardial infarction, cerebrum- vascular events, alteration of enzyma
...

In vitro method for predicting whether a compound is genotoxic in vivo.

The invention is in the field of genomics and it provides an in vitro method for predicting whether a compound is genotoxic in vivo. It provides a method that employs the analysis of expression profiles of primary mouse hepatocytes as an in vitro system to discriminate false GTX compounds from true GTX carcinogens. It was found that differential expression of a number of genes could reliably predict whether a compound was a true genotoxic compound.
...

Homogeneous, Intrinsic Radiopaque Embolic Particles

The invention is directed to embolic material comprising spherical, homogeneous and substantially non-porous radiopaque polymer particles based on at least one hydrophilic monomer and at least one radiopaque monomer according to general formula wherein R is H, methyl or ethyl, and R1 is I, Br or wherein R2 is O, NH, O-[CH2-CH2-O]p-C(O)-, O-[CH2]m-O-C(O)-, O-[CH2]p-, NH-[CH2-CH2-O]p-C(O)-, NH-[CH2]m-O-C(O)- or NH-[CH2]p- wherein m>1 and p>=1, R3 is I or Br and n is 1, 2 or 3, the iodine and/or br
...

Method for determining the primary site of cup

The invention relates to a method for the classification of cancer using a specific multi-class tumor classifier comprising specific sets of genes for the interpretation of expression data obtained from tumor samples. More specifically, the method of the present invention provides an accurate, reproducible, robust, objective and easy to perform method for determining the primary site of a Cancer of Unknown Primary site (CUP). For this purpose, the method provides that a classifier parameter is d
...

Peptide Ligands for Targeting to the Blood-brain Barrier

The present invention relates to peptides comprising defined amino acid sequences, compositions comprising said peptides and a pharmaceutically acceptable substance, use of said peptides as a central nervous system targeting principle in the treatment, prophylaxis or diagnosis of a central nervous system disorders, combinations of said peptides with pharmaceutically acceptable substances, use of said combinations for the treatment, prophylaxis or diagnosis of a central nervous system disorders,
...

High Throughput Method and System for in Vivo Screening

Provided is a method and system for screening chemical compounds or compositions, wherein replicating entities are introduced into the yolk of an (un)fertilized egg or embryo. The method may be extended to elucidate the mechanism-of-action of functional chemical compounds or compositions in the same method and system. The method and system may also be employed for identifying marker genes, marker proteins or marker metabolites.
...

A Biological Microfluidics Chip and Related Methods

A biological microfluidics chip (100) comprising a substrate (102), a microfluidic inlet port (104) defining an opening in a surface of the substrate (102), and a microfluidic outlet port (106) defining an opening in a surface of the substrate (102). The biological microfluidics chip (100) also comprises a plurality of wells (108) extending from a top surface (110) of the substrate, wherein each well (108) is bounded by one or more walls, and an inlet opening (112) and an outlet opening (114) ar
...

Novel mRNA splice variant of the doublecortin-like kinase gene and its use in cancer diagnosis and therapy

Methods for treating neuroblastoma in a subject are provided. The methods include administering to the subject nucleic acid that is capable of causing a significant reduction of the amount of doublecortin like protein (DCL) in the subject. The nucleic acids include antisense oligonucleotides and small interfering RNA.
...

A Cell Cultivation Receptacle and Related Apparatuses

A cell cultivation receptacle (10) comprises a recess layer (11) having formed therein a downwardly extending recess (12) defining an opening (13) on an upper side of the recess layer (11). The recess (12) further defines one or more lateral boundaries (14) that taper inwardly in the downward direction, from the opening (13), to intersect the bottom (16) of the recess. The bottom (16) is defined by a bottom layer (17); and the receptacle (10) a removable closure (18), for the opening, including
...

Methods for Immobilizing Microvesicles, Means and Methods for Detecting Them, and Uses Thereof

The invention provides a method for immobilizing microvesicles to a coated surface using a laminar flow. Further provided are a chip mould for the preparation of microfluidic chips suitable for use in the method and uses thereof. Further provided is a microfluidic chip suitable for use of the method. Further provided is a coated surface suitable for use of the method and uses thereof. Further provided are means and methods for detecting microvesicles and uses thereof for detecting or determining
...

Future Cardiac Event Biomarkers

The present invention relates to the identification of chemokine biomarkers predictive of future acute coronary syndromes including unstable angina pectoris (UAP). The present invention also identifies particular chemokines as potential therapeutic targets for intervention in cardiovascular diseases.
...

Novel Cross-Linkers for Obtaining Structure Information on Molecule Complexes

The present invention describes a novel cross-linker, a method for preparing one or more cross-linked biomolecules, biomolecular complexes of two or more biomolecules, a method for preparing cross-linked fragments from such cross-linked biomolecules and/or biomolecular complexes, a method for cleavage and reduction of such cross-linked biomolecules and/or biomolecular complexes, a method for identifying cross-links in such cross-linked biomolecules and/or biomolecular complexes, as well as a met
...

Synucleinopathies

Present inventions demonstrates that NHE-1 inhibiting carbonylguanidine derivatives inhibit a-synuclein toxicity. Such compounds can be used in the treatment or prevention of synucleinopathies, such as Parkinson's disease (PD), dementia with Lewy bodies (DLB), pure autonomic failure (PAF), and multiple system atrophy (MSA).
...

Mutant Aox 1 Promoters

A mutant Pichia pastoris alcohol oxidase 1 (AOXl) promoter of the wild type Pichia pastoris AOXl promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of : a) a transcription factor binding site (TFBS) , b) nucleotides 170 to 235 (-784 to -719) , nucleotides 170 to 191 (-784 to -763) , nucleotides 192 to 213 (-762 to -741) , nucleotides 192 to 210 (-762 to -744) , nucleotides 207 to 209 (-747 to -745) , nucleotides 214 to 235 (-740 to -719) , nucleotides 304
...

Biomarker for osteoarthritis and/or other ageing-related diseases, and use thereof

The invention relates to the identification of a biomarker whose abundance in bodily fluids is changed in subjects with osteoarthritis and/or other ageing-related diseases. The biomarker has applications in the diagnosis of osteoarthritis and/or other ageing-related diseases, in determining the prognosis for an individual diagnosed with osteoarthritis and/or other ageing-related diseases, and in monitoring the efficacy of treatment for osteoarthritis and/or other ageing-related diseases.
...

Method for the Production of Recombinant Proteins By Green Microalgae

The present invention relates to a method for the production of recombinant proteins by algal cells, which are fresh water unicellular green microalgae belonging to the order of Sphaeropleales. The present invention further provides recombinant algal cells, wherein said algal cells are fresh water unicellular green microalgae belonging to the order of Sphaeropleales,and wherein said algal cells are capable of producing a recombinant protein. The invention is also directed to a method for selecti
...

Biomarker for Osteoarthritis And/or Other Ageing-related Diseases, and Use Thereof.

The invention relates to the identification of abiomarker whose abundance in biologicalsamplesis changed in subjects with osteoarthritis and/or other ageing-related diseases. Thebiomarker hasapplications in the diagnosis of osteoarthritis and/or other ageing-related diseases, in determining the prognosis for an individual diagnosed with osteoarthritis and/or other ageing-related diseases, and in monitoring the efficacy of treatment for osteoarthritis and/or other ageing-related diseases.
...

Aptamers Directed Against the Matrix Protein-1 of Type a Influenza Viruses and Uses Thereof

The present invention relates to nucleic acids that bind specifically to the matrix protein- 1 of type A influenza viruses and uses thereof for detecting such viruses in a sample of interest. More particularly, the present invention relates to a nucleic acid that binds specifically to matrix protein- 1 of type A influenza viruses characterized in that said nucleic acid comprises the following nucleotide sequence: 5'-N1-NS1-U-N3-A-NS3-NS5-NS7-NS6-CGCAU-NS4-C-N4-NS2-N2-3' wherein: - N1 consists of
...

Hiv-rev-multimerization Inhibiting Nanobodies

The present invention relates to an anti-HIV-Rev antibody. More specifically, the invention relates to a nanobody that is able to inhibit the multimerization of the Revprotein, and by this, it inhibits the nuclear export of single-spliced and unspliced HIV-1 m RNA, and can be used as an anti-HIV agent. The invention relates further to the use of the antibody in the screening of compounds that can inhibit HIV-Rev multimerization.
...

Enhancing the T-cells Stimulatory Capacity of Human Antigen Presenting Cells and their Use in Vaccination

With the current invention, we provide new methods of enhancing the T-cell stimulatory capacity of human dendritic cells (DCs) and their use in cancer vaccination. The method comprises the introduction of different molecular adjuvants to human DCs through transfection with at least two mRNA or DNA molecules encoding markers selected from the group of: CD40L, CD70, constitutively active TLR4 (caTLR4), IL-12p70, EL-selectin, CCR7 and/or 4-1 BBL; or in combination with inhibition of SOCS, A20, PD-L
...

Aqueous Coacervate Composition Comprising Poorly Water-soluble Biologically-active Agents

This invention relates to an aqueous gelatin-free, egg portion-free, two-phase coacervate composition comprising a coacervate phase and an equilibrium water phase, comprising, per 100 parts by weight, a mixture of: (a) from 2 to 30 parts by weight of a poorly water-soluble bio-active agent, (b) from 5 to 55 parts by weight of a polysaccharide-free tensio-active system with a HLB from 8 to 18 and consisting of non-ionic tensio-active agents, (c) from 2 to 40 parts by weight of one or more water-s
...

Plant Growth Promoting Protein Complex

The present invention relates to a plant growth promoting protein complex. More specifically, the invention relates to the use of specific proteins from the Anaphase Promoting Complex/Cyclosome for increasing shoot growth rates and/or enhancing cell division rates.
...

Inhibitors of F18+ E Coli Binding

The present invention relates to blood group A/B/H determinant on Type 1 Core glycosphingolipids chains as recognition point for the FedF protein of F18-fimbriated Enterotoxigenic and verotoxinogenic Escherichia coli and the use of compounds comprising such determinants for the treatment of F18+ E. coli infections in pigs and in screening methods.
...

Incorporation of Thermo-resistant And/or Pressure-resistant Organisms in Materials

The present application relates to methods for producing materials having viable micro-organisms incorporated therein. More particularly, the materials are produced under high temperature and/or high pressure conditions, and the micro-organisms are incorporated in the material before or during these conditions. Also provided are micro-organisms that remain viable under these conditions and have different applications, depending on the nature of the material.
...

Substituted 7-azabicyclo[2.2.1]heptyl Derivatives Useful for Making Pharmaceutical Compositions

This invention provides 1-substituted-7-azabicyclo[2.2.1]heptyl derivatives, intermediates and methods for producing them, which are therapeutic agents useful for the prevention and treatment of central nervous system disorders and diseases mediated by a Nicotinic Acetylcholine Receptor such as Alzheimer's disease, Parkinson's disease, schizophrenia, epilepsy, pain, nicotine addiction and dementia.
...

Cationic Liposomes for the Delivery of High Molecular Weight Compounds

The present invention relates to a specific type of liposomes, denominated as SECosomes, made from a cationic lipid such as 1, 2-dioleoyl-3-trimethylammonium propane (DOTAP), a surfactant such as sodium cholate (NaChol), a stabilizer such as cholesterol or a derivate thereof, and, an alcohol such as ethanol. Said liposomes are capable of delivering high molecular weight compounds, such as siRNAs, efficiently across tissues and into cells of tissues, such as skin.
...

Methods and Compositions in the Treatment of Coronaviruses

The invention relates generally to the field of virology, and relates to the identification and characterization of the targets involved in the evasion strategy of viruses and to the use thereof in methods to identify anti-viral compounds. More in particular to identify compounds which are modulators of myosin light chain kinase (MLCK), a target within said entry and immune-evasion strategy.; Other aspects of the invention are directed to anti-viral compounds identified using the models and meth
...

Liver-specific Nucleic Acid Regulatory Elements and Methods and Use Thereof

The present invention relates to nucleic acid regulatory elements that are able to enhance liver-specific expression of genes, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy.
...

Methods and Systems for Channel Selection

A system and method are described for estimating a property of a neural or cardial source using inverse problem solving including a forward numerical model. The system comprises a selection means for selecting at least one subset of a plurality of measurement channels, the selection taking into account the sensitivity of the measurement channel results to conductivity in the forward numerical model for the neural or cardial source. The system also comprises a calculation means for determining a
...

Methods for Producing Substantially Homogeneous Hybrid Or Complex N-glycans in Methylotrophic Yeasts

The present invention provides methods for effectively and efficiently converting methylotrophic yeast's heterogeneous high mannose-type N-glycosylation to mammalian-type N-glycosylation by disruption of an endogenous glycosyltransferase gene (OCH1) and step-wise introduction of heterologous glycosidase and glycosyltransferase activities. Each engineering step includes a number of stages: transformation with an appropriate vector, cultivation of a number of transformants, performance of sugar an
...

Cells Producing Glycoproteins Having Altered Glycosylation Patterns and Methods and Use Thereof

The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells and methods are glyco-engineered y
...

Minichromosome Maintenance Complex Interacting Protein Involved in Cancer

The present invention relates to a protein that is interacting with the minichromosome maintenance complex in eukaryotes. More specifically, the invention relates to the use of a protein, interacting with the minichromosome complex, and the gene encoding this protein in diagnosis, prognosis and treatment of cancer.
...

A Proteolytic Product of Histone H2A As a Marker for Cancer

The present invention relates to proteolytic products of histone H2A. In particular, the present invention discloses a peptide having the amino acid sequence VTIAQGGVLPNIQAV which can be used to determine cleavage of histone H2A. More in particular, the present invention relates to the latter peptide as a marker to diagnose or to determine prognosis of cancer such as chronic lymphocytic leukemia.
...

Novel Immunotherapy Strategy

The present invention relates to polyacetal-carboxylic acids, more in particular chlorite-oxidized oxyamylose (COAM) for use as a medicine, more in particular for the treatment or prevention of cancer and auto-immune disorders or relates to the use of polyacetal-carboxylic acids for the manufacture of a medicament for the prevention or treatment of cancer or auto-immune disorders. The invention provides also pharmaceutical compositions comprising said polyacetal-carboxylic acids and method of pr
...

Activators of the Autophagic Pathway

By present invention it has been clearly demonstrated that polyamine compounds of the groups consisting of putrescine, spermine, spermidine, cholesteryl spermine, spermidine trihydrochloride, spermidine phosphate hexahydrate, spermidine phosphate hexahydrate and 1,4-butanediamine N-(3-aminopropyl)-monohydrochloride or derivatives thereof or combinations thereof can induce deacetylation or can maintain a condition of hypoacetylation of histone H3 and can activate autophagy and can be used in a tr
...

HSC Self-renewal

The invention is related to methods for culturing stem cells, more particularly hematopoietic stem cells (HSC). The invention relates to methods for HSC expansion and the use of factors to increase the retention and/or expansion of KLS cells in vitro. The invention is also directed to cells produced by the methods of the invention. The cells are useful, among other things, for treatment of disorders or diseases (e.g. leukemia). The invention also relates to the development of small molecules tha
...

Functional Layers of Biomolecules and Living Cells, and a Novel System to Produce Such

The present invention concerns a new process for depositing a thick compact layer of biomolecules for instance such a layer with thickness in the [mu]m scale and, for depositing a thick compact layer of cells in the [mu]m scale. The deposited layer is made by application of an unbalanced (asymmetrical) alternating voltage polarization between two electrodes to a dissolved biomolecule or cell from low conductivity solutions. The process allows the rapid manufacturing of sensors and the coating of
...

Use of Nucleoside Analogs As Anti-koi Herpes Virus Molecules

The present invention relates to the use of substituted or unsubstituted bis(hydroxymethyl)-cycloprop-1'-ylmethyl nucleobase derivatives as active agents for the treatment or prevention of Koi Herpes virus infections in fish, especially in carps.
...

Insertion Sequence Detection Protocol

The present invention relates to a novel microarray-based methodology, referred to as 'Sliding Window Hybridization' (SWH), for reliable and convenient detection of short nucleic acid sequences in genomes of an organism.
...

Hepatocellular Carcinoma

Present invention concerns a kit and an in vitro method, for evaluating a biological stage of an HCC tumour in an individual, based on a sample from the individual, comprising: deriving from the sample a profile data set, the profile data set on a the gene expression panel with the markers CCNG2, EGLN3, EROlL, FGF21, MATlA, RCLl and WDR45L or a substantially similar marker, being a quantitative measure of the amount of a distinct RNA or protein constituent in the panel so that measurement of the
...

Novel Phosphate Modified Nucleosides Useful As Substrates for Polymerases and As Antiviral Angents

This invention provides phosphate-modified nucleosides represented by the structural formula: wherein W is O or S, and wherein B, R1; R3 and R2. are s defined herein. These compounds are useful as substrates for DNA/RNA polymerases, and as anti-viral agents in particular against HIV-1.
...

Indole Amide Derivatives and Related Compounds for Use in the Treatment of Neurodegenerative Diseases

This invention provides novel compounds and the novel compounds for use as a medicine, more in particular for the prevention or treatment of neurodegenerative disorders, more specifically certain neurological disorders, such as disorders collectively known as tauopathies, and disorders characterised by cytotoxic a-synuclein amyloidogenesis. The present invention also relates to the use of said novel compounds for the manufacture of medicaments useful for treating such neurodegenerative disorders
...

Stem Cell Aggregates and Methods for Making and Using

The invention is directed to compositions of cell aggregates and methods for making and using the cell aggregates where the aggregates comprise cells that are not embryonic stem cells but can differentiate into cell types of at least two of ectodermal, undo dermal, and mesodermal embryonic germ layers, e.g., stem cells.
...

New Endolysin Obpgplys

The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO: 1 and fragments or derivatives thereof. The present invention further relates to fusion proteins comprising said polypeptide and an additional peptide stretch fused to said polypeptide at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either
...

Antimicrobial Agents

The present invention relates to endolysin variants comprising an endolysin to which a peptide stretch with membrane or LPS disrupting activity is fused. Moreover, the present invention relates to nucleic acid molecules encoding said modified endolysin variant, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to a method for producing said endolysin variant. Further, the present
...

Method for Inactivating At Least One Pathogen in Human Blood Plasma Sample

The present invention relates to a method for inactivating at least one pathogen of a human blood plasma sample, including: adiabatically compressing said sample, the temperature of said plasma being, at a pressure P1, between -10 DEG C and -3 DEG C as a result of the compression; optionally maintaining the pressure P1 for a time of 110 to 130 seconds; and adiabatically expanding the sample. The present invention also relates to the use of the method of the invention and to the plasma obtained b
...

Rocaglaol Derivatives As Cardioprotectant Agents and As Antineoplastic Agents

The present invention discloses new rocaglaol derivatives and the use of rocaglaol derivatives to prevent or to limit the cardiotoxicity of an antineoplastic agent, in particular to prevent or to limit the apoptosis of cardiomyocytes induced by such agent.
...

Mechanically Responsive Supported Polyelectrolyte Multilayer Films

The invention concerns a mechanically responsive supported polyelectrolyte multilayer film, comprising: - an elastic substrate, on which are alternately coated: - at least one exponentially growing film; and - at least one mechanically responsive barrier film. It further concerns a process for its preparation and the use of such films notably for mechanically responsive mixing or delivery of active substances. It further concerns dosage forms of a drug, biosensors and implants comprising such fi
...

Biologically Active Compositions, Methods for Producing the Same and Biological Applications

Use of a product from plants comprising extracts, fractions and/or molecules as G-protein coupled receptor (TGR5) specific agonist, having minimum effective concentration (EC50) of greater/equal to TGR5 natural ligand comprising litocholic acid, not activate the farnesoid X receptor (FXR) nuclear receptor, inducing a reduction of weight gain induced by a fatty food to a model, who has metabolic syndrome, and a reduction of mass of fatty cell of epididymis in the models, and inducing a improvemen
...

Tocopherol Derivatives with a Long Hydroxylated Chain, which Can Be Used As Neurotrophics

La présente invention a pour objet tout composé isolé ou synthétique, et en particulier les composés de formule (I), provoquant la différentiation de cellules précurseurs d'oligodendrocytes, ainsi que la modulation de l'activation de la microglie. Elle concerne également les méthodes de préparation de tels composés ainsi que l'utilisation desdits composés dans le cadre de la préparation d'une composition pharmaceutique destinée à la prévention ou au traitement de maladies affecta
...

Monoclonal Anti-claudin 1 Antibodies for the Inhibition of Hepatitis C Virus Infection

The present invention provides monoclonal antibodies that specifically bind to the extracellular domain of human Claudin-1 on the cell surface, thereby inhibiting HCV entry into susceptible cells and preventing HCV infection of these cells; and hybridoma cell lines which produce such monoclonal antibodies. Also provided are reagents that comprise such antibodies, and pharmaceutical compositions comprising such antibodies. Methods of treating or preventing HCV infection by administration of an in
...

Thin-layer Photobioreactor with High Volume Productivity

The invention relates to a photobioreactor (1) for the culture of cells, comprising a runoff substrate (2) having a surface inclined at an average slope along a direction of inclination (I-I) on which a solution flows and including an upstream side (21) and a downstream side (22), said solution comprising cells in suspension. The invention is characterised in that the photobioreactor (1) comprises an upflow element (3) that conveys the solution from the downstream end (22) to the upstream end (2
...

Novel Melanoma Antigen Peptide and Uses Thereof

The present invention relates to novel melanoma antigen peptides and specific T lymphocytes directed to said peptides and the use thereof for treating melanoma.
...

Method for making biochips

A method for immobilizing biological polymers such as DNA or proteins, on a solid support, by ionocovalent bond, for making biochips, and the resulting chips obtained by the method.
...

Cellular Differentiation Process and Its Use for Blood Vessel Build-up

The present invention relates to the use of specific oxygen concentrations for implementing a process of differenti-ation of stem, provided that said stem cells are not humanembryonic stemcells, and seeded on a support, in an appropriate culture medium, wherein said differentiation leads to: -either a first group of specialized differentiated cells under normoxic conditions, and in an appropriate culture medium, -or a second group of specialized differentiated cells under hypoxic conditions, in
...

Mutated Hyperthermophilic Phosphotriesterases and Uses Thereof

A subject of the present invention is mutated hyperthermophilic phosphotriesterases (PTEs) possessing a lactonase activity, and their uses as bioscavengers within the context of the decontamination of the surfaces of materials, of the skin or mucous membranes, contaminated with organophosphorus compounds, or within the context of the preparation of medicaments which can be used within the context of the prevention or treatment of an external contamination or of an internal poisoning by ingestion
...

Therapeutic Peptides and Method

A polypeptide comprising one or more sequences derived from CDR2 or CDR3 of a TREM-1 protein, characterised by the ability to treat, ameliorate, or lessen the symptoms of sepsis, septic shock or sepsis-like conditions.
...

Peptide useful as an antimicrobial agent has a defined sequence of 14 amino acids

Antimicrobial peptide (I) has a defined sequence of 14 amino acids. Peptide (I) with antimicrobial activity has the amino acid sequence Lys Lys Lys Lys Pro Leu Phe Gly Leu Phe Phe Gly Leu Phe. INDEPENDENT CLAIMS are also included for: (1) polypeptide (II) comprising the above sequence; (2) polynucleotide coding for (I) or (II); (3) cloning or expression vector containing the polynucleotide; (4) nonhuman host organism transformed with the vector. - ACTIVITY : Antibacterial. - MECHANISM OF ACTION
...

C4d/C4b Standard for Quantitative Flow Cytometry of Humoral Transplant Rejection

The invention concerns an internal standard used to quantitative analysis of the risk of humoral (i.e. vascular) transplant rejection. The internal standard consists of a stable composition of the C4d complement bound to a carrier consisting of erythrocytes or microparticles. The invention also concerns a method for analyzing in vitro the risk of humoral organ transplant rejection, which consists in determining the amount of component of C4d component fixed on the erythrocytes contained in a blo
...

Use of Centrifugal Partition Chromatography for Purifying Galanthamine

The invention concerns the use of centrifugal partition chromatography in displacement mode for implementing a process for purifying galanthamine or its derivatives, from a starting composition, containing at least 20% of galanthamine or its derivatives. Said method comprises a step of centrifuging a combination of at least two solvents and said starting composition, for a time sufficient for purifying the galanthamine or its derivatives, said solvents being such that they form two non-miscible
...

Methods and Systems for Expanding AC133+ Cells and Directing Differentiation

The invention provides, among other things, methods and systems for expanding CDl 33+ cells. The invention further provides methods and systems for increasing the blood flow to an ischemic tissue in a subject in need thereof, such as to ischemic myocardium. The invention further provides methods and systems for directing differentiation of expanded CD 133+ cells. The invention further provides methods and systems for treating a subject with differentiated cells in a subject in need thereof. The
...

Method of Identifying Micro-organisms and their Species in Blood Culture

The invention relates to a method of identifying micro-organisms and their species in a blood culture by mass-spectrometry and to a device for implementing such a method.
...

4S-iota-carrageenan Sulfatase and Use Thereof to Obtain Alpha-carrageenan

The present invention relates to 4S-iota-carrageenan sulfatase and to the use thereof for partially or totally converting the iota-carrageenan into iota-/alpha-carrageenan or alpha-carrageenan. The invention also relates to the method for extracting said enzyme from a Pseudoalteromonas bacteria population or from red marine algae. Finally, the present invention relates to the use of 4S-iota-carrageenan sulfatase to prepare a texturizing agent containing alpha-carrageenan.
...

Method for Detecting a Circularized DNAa, and Use of Said Method for Detecting Mutations

The present invention relates to a method for detecting a circularized single-stranded DNA by means of the isothermal hyperbranched rolling circle amplification technique, in which the primers used comprise a detectable barcode sequence and, optionally, a spacer which blocks polymerization by DNA polymerase. The present invention also relates to the use of said method for detecting a genetic polymorphism of one or more base pair(s).
...

Leptin Antagonists

The invention relates to synthetic leptin antagonists in which at least two amino acid residues of the sequence LDFI/S of the hydrophobic binding site at positions 39-42 of a leptin polypeptide sequence are substituted with different amino acid residues such that the site becomes less hydrophobic, and fragments of said leptin antagonists.
...

Nucleic Acid Molecules and Methods for Exchanging Exon(s) By Transsplicing

The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosome mediated simple or double trans-splicing. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre- mRNA) and to mediate a simple or double trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA).
...

A Stimulatable Asymmetrical Polymersome

The invention relates to a polymer vesicle with an asymmetrical unilamellar membrane, said membrane being constituted by two distinct layers, a layer (A) and a layer (B), said layers comprising respective amphiphilic block copolymers with layer (A) comprising, in contrast to said layer (B), an effective quantity of amphiphilic block copolymer comprising at least one stimulatable hydrophobic polymer block, said block polymers provided by said copolymers being capable of adopting a new steric conf
...

Non-auxotrophic Selection Marker

A non-auxotrophic selection marker located in a gene cluster encoding a metabolite for an organism, such as a fungus, lacking said marker is provided. The selection marker gene can be used to detect and confirm the introduction of foreign genes without the use of exogenous drug-resistant genes to facilitate transformant selection. The selection marker gene can be applied to detect the presence of fungal infection and predict the likelihood of the development of disease or allergic response at an
...

Method for Detecting Phosphopantetheinyl Transferase Activity

The invention provides a method for detecting 4 -phosphopantetheinyl transferase (4 PPTase) activity, the method comprising the steps of: (a) immobilising a carrier protein domain or a functional fragment thereof on a solid support suitable for this purpose; (b) reacting the immobilised carrier protein domain or functional fragment thereof of step (a) with labelled coenzyme A and 4' PPTase to form a labelled carrier protein or functional fragment thereof; and (c) detecting the presence of the la
...

A Method for Screening Compounds

A method of screening compounds or molecules comprising the steps of: translating a sequence encoding the amino acid sequence comprising SEQ ID No. 29 in a translation system in the presence of a test compound or molecule; and analysing the translation product(s) for the presence of one or more of (a) a peptide comprising the amino acids Pro-Gly at the C terminus and a peptide comprising the amino acid Pro at the N terminus: or (b) a peptide comprising the amino acid sequence of SEQ ID No. 29.
...

Modulation of tissue fatty acid composition of a host by human gut bacteria

The current invention provides use of a CLA - producing bacterium for the in vivo conversion in the gut of polyunsaturated fatty acids to CLA. The CLA-producing bacterium is selected from one or more of the group consisting of propionibacteria, lactobacilli, lactococci and streptococci, and bifidobacteria.
...

Orally Administered Bacteria As Vehicles for Systemic Delivery of Agents

The current invention relates to the use of a bacterial species in the preparation of a composition adapted for oral administration for the delivery of an agent to a site in the body. The site in the body may be an organ or a tumour site. The bacterial species is a food grade, non-pathogenic, gram-positive bacteria capable of anaerobic growth.
...

Method for Opening Tight Junctions

The present invention is directed to a method and use of RNA interference (RNAi) for the transient, reversible and controlled opening of the tight junctions of the blood brain barrier and/or the blood retinal barrier. This method may be used in the treatment of many diseases and disorders which require the opening of the blood brain barrier and/or blood retinal barrier. Such methods generally involve the use of an RNAi-inducing agent, such as siRNA, miRNA, shRNA or an RNAi-inducing vector whose
...

Cytokines As Prognostic Markers of Respiratory-tract Infection Following Major Surgery

The invention relates to the use of a certain subset of cytokine markers as prognostic variables of infection status in an individual, and especially as prognostic markers of a patients developing severe infection such as pneumonia, and respiratory tract infection following surgery. The subset of cytokine markers consists of the interleukin cytokines IL-2, IL-7, IL-23, IL-27, and IL-IO, and Interferon-? (INF?) and Tissue Necrosis Factor-a (TNFa). The markers may be employed as individual prognos
...

A Method of Estimating Sepsis Risk in an Individual with Infection

A method of estimating sepsis risk in an individual with infection comprises a step of assaying a biological sample from the individual for an IL-2 or IL-7 mRNA value, and correlating the mRNA value with sepsis risk. The IL-2 and IL-7 mRNA values are quantified by absolute quantification of mRNA copy number, wherein the copy numbers are normalised to a house keeping gene and corrected against a calibration curve for serial dilutions of the IL-2 and IL-7 cDNA. The method generally involves a step
...

RNAi in Budding Yeast

The invention provides budding yeast that have a functional RNAi pathway. The invention provides RNAi pathway polypeptides derived from budding yeast that have an endogenous RNAi pathway. In some embodiments the invention provides functional budding yeast Dicer polypeptides and variants thereof. In some embodiments the invention provides functional budding yeast Argonaute polypeptides and variants thereof. Also provided are isolated nucleic acids encoding the polypeptides of the invention, vecto
...

Method for Producing Polyhydroxyalkanoate

A method for producing polyhydroxyalkanoate (PHA), comprising (i) culturing in a culture medium comprising terephthalic acid and/or a salt thereof and/or an ester thereof one or more bacterial strains which are capable of accumulating PHA from terephthalic acid or a salt or ester thereof and which are selected from Pseudomonas putida strain GO 16 having the accession number NCIMB 41538, Pseudomonas putida strain GO 19 having the accession number NCIMB 41537, and Pseudomonas frederiksbergensis st
...

Pseudomonas Putida Styrene Monooxygenase Variants

The present invention provides isolated polynucleotides derived from Pseudomonas putida, and encoding polypeptides having styrene monooxygenase activity. The polynucleotides of the present invention are derived from the styAB gene from Pseudomonas putida CA-3, using error prone PCR-based in vitro evolution. The polypeptides encoded by these polynucleotides are useful as biocatalysts for the synthesis of oxygenated compounds, as they exhibit improved properties, such as enzymatic activity and ena
...

A Method for Predicting Athletic Performance Potential

A method and assay for predicting athletic performance potential of a subject, such as a thoroughbred race horse, comprising the steps of assaying a biological sample from a subject for the presence of a single nucleotide polymorphism in one or more genes associated with athletic performance. The athletic performance genes may be selected from one or more of MSTN, COX4I2, PDK4, CKM and COX4I1.
...

A Method of Immobilising Biological Molecules to a Support and Products Thereof

A support comprising a substrate, wherein a surface of the substrate comprises functional amino or aldehyde groups, for the covalent binding of a biomolecule. The biomolecule may be a protein such as an affinity protein. The invention also provides a method for the covalent binding of a biomolecule to a surface of a support through its amino or carboxyl group. The method comprises the steps of: providing a support substrate; cleaning a surface of a support substrate; functionalising the support
...

Anti-sialic Acid Antibody Molecules

A method of generating and isolating a recombinant high affinity anti-sialic acid antibody molecule comprises the steps of immunising a host with an immunogen comprising a conjugate of sialic acid and a carrier protein to generate an anti-sialic acid polyclonal serum, isolating a sample of RNA from the immunised avian host, and generating and screening of a library of recombinant antibody molecules from the RNA sample, and isolating a recombinant high affinity anti-sialic acid antibody molecule.
...

A Multi-well Plate for Biological Assays

A multi-well plate (1) for a biological assay comprising a plate frame (4) attached to a support substrate (7) through a gasket (5) wherein the plate frame (4) defines side walls for a plurality of wells (2) and the gasket (5) comprises a plurality of holes (6), whereby the holes (6) of the gasket (5) are substantially aligned with the wells (2) defined by the plate frame (4) such that the support substrate (7) forms a base for the wells (2) and wherein the side walls of the wells (2) comprise a
...

TFF3 Hypomethylation As a Novel Biomarker for Prostate Cancer

The present invention relates to a TFF3 gene as a marker of prostate cancer. The present invention thus relates to methods for diagnosing prostate cancer or predicting or prognosticating the disease outcome comprising steps of determining the methylation status of a TFF3 gene and/or the transcriptional and translational expression levels of a TFF3 gene. The present invention further relates to methods for monitoring disease progression in an individual having contracted prostate cancer as well a
...

Selective Exosite Inhibition of PAPP-A Activity Against IGFBP-4.

The present invention relates in one embodiment to PAPP-A exosite(s) interactors such as antibodies which bind to a region comprising LNR3 of PAPP-A and efficiently inhibit proteolysis of IGFBP-4, but not -5. The region comprising LNR3 represents a substrate binding exosite, which can be targeted for selective proteolytic inhibition. Accordingly, the present invention relates in one embodiment to differential inhibition of natural protease substrates by exosite targeting.
...

Biocompatible Materials for Mammalian Stem Cell Growth and Differentiation

A biocompatible material, wherein at least a part of a surface of the biocompatible material is characterized by a micro or nano-meter scale topographical structure comprising a plurality of features where the structure is selected to promote the growth of undifferentiated pluripotent stem cells or serve to promote the uniform differentiated growth of stem cells. Furthermore, a biocompatible material is provided having a surface structure and composition that affects a cellular function, in part
...

Modified Peptides As Potent Inhibitors of the PSD-95/NMDA Receptor Interaction

The present invention is directed to the provision of small molecule inhibitors of the PSD-95/NMDA receptor interaction, employing an undecapeptide corresponding to the C-terminal of the NMDA as a template for finding lead candidates. A compound (NMDAR/PSD-95 inhibitor) of the invention includes a peptide or peptide analogue comprising at least four peptide bonded residues having the sequence YTXV or YSXV, wherein Y is selected from among E, Q, and A, or an analogue thereof, and X is selected fr
...

Use of Novel Biomarkers for Detection of Testicular Carcinoma in Situ and Derived Cancers in Human Samples

The present invention relates to methods and kits for identification of testicular carcinoma in situ (CIS), gonadoblastoma (a CIS-like pre-cancerous lesion found in dysgenetic gonads) and CIS-derived cancers based on at least one of the biomarkers included in the invention. It also relates to diagnosis of a subject's status of the testicular carcinoma in situ and the derived cancers based on the measurement of a relative abundance of one of the biomarkers.
...

Dna-based Methods for Clone-specific Identification of Staphylococcus Aureus

MRSA CC398 is a clone of S. aureus that has recently emerged in pigs and other domestic animals worldwide. As any other MRSA, the clone displays high levels of antibiotic resistance and poses a serious threat to human health because of the risk of antibiotic treatment failure in human patients. We developed a new diagnostic test for identification of MRSA CC398 using a single one-step PCR that is very easily performed within a few hours. The test is based on the principle that clonal differences
...

Biosynthetic Engineering of Glucosinolates

The invention provides methods and materials, such as newly characterised genes, and novel processes, for converting a host from a phenotype whereby the host is unable to carry out glucosinolate (GSL) biosynthesis or chain elongation from an amino acid GSL-precursor to a phenotype whereby the host carries out said biosynthesis or elongation.
...

Porcine polymorphisms and methods for detecting them

Identification of a pig as resistant or non-resistant to enterotoxigenic E. Coli (ETEC). Particularly, there is provided methods, probes and DNA molecules involved in identifying a pig as resistant or non-resistant to ETEC. There is also provided methods for breeding pigs using the information of resistance/non-resistance, mixed boar semen, and methods for developing drugs to compensate for non-resistance to ETEC.
...

A Nucleotide Vaccine

The present invention relates to a vaccine comprising a nucleic acid construct such as a DNA construct especially a nucleic acid construct comprising sequences encoding invariant chain operatively linked to antigenic protein or peptide encoding sequences. The vaccine stimulates an immune response, especially an immune response in an MHC-I dependent, but CD4<+> T-cell independent manner.
...

Thermostable DNA polymerase of the archaeal ampullavirus ABV and its applications

The present invention is directed to the thermostable DNA polymerase protein of the archaeal ampullavirus ABV (Acidianus Bottle-shaped virus) and the nucleic acid encoding said DNA polymerase. The invention also relates to method of synthesizing, amplifying or sequencing nucleic acid implementing said DNA polymerase protein and kit or apparatus comprising said DNA polymerase protein.
...

Flavin Monooxygenases and Transcription Factors Involved in Glucosinolate Biosynthesis

The invention provides methods and materials relating generally to plant derived flavin-containing monooxygenases (FMOs) capable of catalysing oxidation of a thio- to a sulphinyl- group during glucosinolate biosynthesis. It further relates to plant derived MYB factors capable of transcriptional regulation of biosynthetic genes. These have utility in the modification of glucosinolate biosynthesis.
...

Analysing Breath Samples for Volatile Organic Compound

The invention describes the use of a biomarker pentylfuran to detect bacterial and/or fungal pathogens. Pentylfuran is released from certain pathogens and detected in the headspace gas of an in vitro culture or in the breath sample of a patient. Pentylfuran is particularly useful in the detection of Aspergillusspecies, especially Aspergillus fumigatus which is a pathogen of humans.
...

Nitric Oxide Donors

The invention relates to novel NO donors which are targeted to the mitochondria. The NO donor compounds of the invention allow NO to be selectively provided to the mitochondria.
...

Detection of Volatile Compounds As Markers for Mycobacteria Tuberculosis

The invention relates to the use of biomarkers for identifying bacterial pathogens. In particular, the invention relates to the detection and identification of certain compounds as markers for mycobacterial infections in a patient or from a culture by analysis of a gaseous sample.
...

Anti-cancer Compositions Containing a Shark Meat Extract and a Mushroom Extract

The invention relates to anti-cancer compositions comprising synergistically effective amounts of an extract of shark meat and an extract of a mushroom, in particular, compositions containing a shark meat extract and an extract from the Korean mushroom Phellinus linteus. The invention also relates to a method of treating or preventing cancer, particularly melanoma, using a composition of the invention.
...

Fusion Polypeptides and Uses Thereof

The invention relates to fusion polypeptides comprising a polynucleotide-binding domain, such as a DNA-binding domain, and a ligase domain, such as a DNA ligase domain, methods for the production of such fusion polypeptides, and uses of the fusion polypeptides, for example in a range of molecular biological techniques as well as applications in the diagnostics, protein production, pharmaceutical, nutraceutical and medical fields.
...

Immunogenic polypeptide isolated from mycobacterium avium subspecies paratuberculosis and uses thereof

The present invention relates to an immunogenic polypeptide isolated from Mycobacterium avium subspecies paratuberculosis and variants of the polypeptide. The polypeptide and variants may be used in vaccines against Johne's disease and in methods for detection of the disease. Antibodies against the polypeptide or variants may be used in diagnostic tests for Johne's disease. Also included are polynucleotides encoding the polypeptide and variants, and methods for preparing these.
...

Membrane Protein Expression System and Its Application in Drug Screening

PROBLEM TO BE SOLVED: To provide a convenient in vitro membrane protein expression system using an inhibitor of drug efflux pump proteins and heterologous cells for testing other drug resistance-relating cell membrane proteins. ; SOLUTION: Provided is a protein expression system comprising an in vitro cell-based expression system for over-expression of a heterologous protein having efflux pump action relating to the drug resistance of a host cell membrane for drug screening applications, that is
...

Peptoid compounds

The invention relates to new peptoid compounds of formula (I), as well as their use in the treatment of bacterial infections, such as those caused by vancomycin resistant microorganisms, and to compositions thereof.
...

Crystal Structure of a Bacterial Enzyme and Uses Thereof

The present invention is predicated, in part, on the determination of the crystal structure of Staphylococcus aureus biotin protein ligase. The present invention provides, among other things, the crystal structure of Staphylococcus aureus biotin protein ligase complexed with biotinyl 5'-adenylate at a resolution of 2.6 Angstroms. Accordingly, the present invention also provides methods for the identification and/or rational design of antagonists and agonists of biotin protein ligase enzymes, and
...

Dual Functioning Ionic Liquids and Salts Thereof

Disclosed herein are ionic liquid compositions comprising active pharmaceutical, biological, and nutritional compounds, and methods of use. Further disclosed are compositions of matter including liquid ion pairs alone or in solution and their use; compositions of ionic liquids that are 'solvated,' for example, 'hydrated' and their uses.
...

A Neural Analysis System

A neural analysis system, including: a neural event extractor for generating Sp/Ap curve data and field potential data for background and initial response segments obtained from a person; a correlator for correlating the Sp/Ap curve data and field potential data with pathology data for a condition, and generating biomarker data points for axes of a biomarker display; and a display module for providing display data to generate the display with the points for use in assessing the person relative t
...

Navigable System for Catheter Based Endovascular Neurosurgery

A navigable system for catheter based endovascular neurosurgery including: a micro-motor (3) including a piezoelectric actuator (5) mounted to a free end of a guidewire or micro-catheter (2), the piezoelectric actuator (5) including a piezoelectric element (7), and an elongate transducer (9) mounted on the piezoelectric element (7) and extending therefrom, the transducer (9) being formed from an asymmetric hollow member, and an end member (15) located at a free end of the transducer (9); wherein
...

Testing Device for Identifying Antigens and Antibodies in Biofluids

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody
...

Anti-angiogenic agents and methods of their use

The present invention relates to the use of sFRP-4 and functional variants thereof as anti-angiogenic agents. It relates to methods of treatment and screening methods using sFRP-4 and functional variants thereof. Further, it relates to compositions, and in particular pharmaceutical composition, including sFRP-4 and functional variants thereof which may be used in the various methods provided herein.
...

A Collagen Scaffold for Cell Growth and a Method for Producing Same

A bioscaffold and method of manufacture is described. The bioscaffold comprised greater than 80% type I collagen fibers or bundles having a knitted structure providing tensile load strength. The method of manufacture comprises the steps of: (a) isolating collagen fibers or bundles; (b) incubating said fibers or bundles in a mixture of NaOH, alcohol, acetone, HCl and ascorbic acid; and (c) mechanical manipulation of said fibers or bundles to produce a knitted structure.
...

Method for Predicting Engraftment Potential

The invention relates to methods for the identification of proliferating stem cells in products for transplantation, and uses thereof. More specifically, the invention relates to methods for predicting the engraftment potential of stem cells following transplantation by determining their proliferative capacity.
...

Classification of Biological Samples Using Spectroscopic Analysis

A method and system is described for rapidly classifying a sample of a biological fluid, comprising obtaining a spectrum of the biological fluid in response to excitation of the sample in a specified frequency range, and applying a multivariate classifier to one or more spectral regions of the spectrum to classify the biological sample into one class in a set of classes, the classes comprising at least two disease states having similar clinical symptoms. Methods and systems for developing the cl
...

Injectable Biomaterials

Injectable biomaterial compositions formed from tropoelastin for tissue repair and restoration. The compositions include a coalescence-controlling agent in the form of a polysaccharide or polysaccharide derivative, in an amount effective for providing the substance with the properties of flow, enabling injection.
...

Therapeutic and Diagnostic Molecules

The present invention relates to methods for modulating angiogenesis, comprising administering to a subject, or cells or tissue derived therefrom: (i) one or more miRNA, or precursors or variants thereof, wherein at least one of said miRNA comprises a seed region comprising the sequence UCACAGU (SEQ ID NO:37) to inhibit angiogenesis; or (ii) one or more antagonists of a miRNA, wherein said miRNA comprises a seed region comprising the sequence UCACAGU (SEQ ID NO:37) to promote or induce angiogene
...

A Novel Regulator of Cellular Senescence

Provided herein are methods for regulating whether a cell undergoes apoptosis or senescence, comprising modulating the level of expression an/or activity of SENEX in the cell, wherein increasing the expression and/or activity of SENEX relative to normal endogenous levels induces cellular senescence and decreasing the expression and/or activity of SENEX relative to normal endogenous levels induces apoptosis. Methods for the diagnosis and treatment of senescence-associated and apoptosis-associated
...

Biocompatible Material and Uses Thereof

The present invention relates to a biocompatible ceramic material comprising Sr, Mg or Ba doped Hardystonite (Ca2ZnSi2O7 ), and a method for its synthetic preparation. The present invention also relates to an implantable medical device comprising biocompatible doped Hardystonite, and a method for its production. The present invention further relates to a method for improving the long term stability of an implantable medical device and an implantable drug delivery device comprising doped Hardysto
...

Enhancement of Microbial Colonization of the Gastrointestinal Tract

PROBLEM TO BE SOLVED: To provide a composition not only transporting probiotic microorganism to the large bowel but also containing a medium functioning to promote the growth of microorganisms when arriving at the large bowel. ; SOLUTION: The probiotic composition includes a carrier which will function to transport one or more probiotic microorganisms to the large bowel of animals or other regions of the gastrointestinal tract of an animal, the carrier comprising a modified or unmodified resista
...

Method of screening multiply transformed cells using bicistronic expression of fluorescent proteins

A method of screening multiply transformed/transfected cells to identify those cells expressing at least two peptides or proteins of interest. The method comprising: 1. Simultaneously or sequentially transforming a cell with at least two different expression cassettes in which the gene of interest is linked via an IRES to a fluorescent marker gene. Each marker gene is different. 2. Providing conditions in which expression of the genes will occur. 3. Identifying cells expressing proteins by detec
...

A Method of Transfection and Compositions Therefor

The present invention relates to the targeted delivery of molecules to cells expressing toll-like receptors (TLRs). Aspects of the invention provide compounds comprising a positively charged group linked to a TLR ligand. These compounds are useful for in vitro and in vivo methods of transfection of TLR-expressing cells. Other aspects of the invention relate to the use of such compounds for repression of gene expression and DNA vaccination approaches.
...

An Assay for Monitoring a Neurological Condition

The present invention relates generally to biomarkers of neurological conditions including monitoring disease progression and the clinical severity of symptoms. More particularly, the present invention provides an assay to facilitate diagnosis and monitoring of progression of neurological conditions and treatment response and to identify targets for therapeutic intervention. Kits and assays for diagnosis and identification of medicaments also form part of the present invention.
...

Polysaccharide Synthases (h)

The present invention relates generally to polysaccharide synthases. More particularly, the present invention relates to (1,3; 1,4)-ss- D-glucan synthases. The present invention provides, among other things, methods for influencing the level of (1,3; 1,4)-ss-D-glucan produced by a cell and nucleic acid and amino acid sequences which encode (1,3; 1,4)-ss- D-glucan synthases.
...

Immunomodulating Compositions Comprising Interleukin 13 Inhibitors and Uses Therefor

This invention relates generally to compositions and methods for modulating immune responses. More particularly, the present invention relates to the co-expression, co-location or co-presentation on host cells (e.g. antigen-presenting cells, leukocytes, etc) of an inhibitor of IL-13 function and an immune stimulator that stimulates an immune response to a target antigen in compositions and methods for stimulating protective or therapeutic immune responses to the target antigen. The compositions
...

UDP-galactopyranose Mutase from Haemonchus Contortus, and RNAi Agents That Inhibit It

The invention relates to the cloning of the Haemonchus contortus UDP-galactopyranose mutase gene, which is homologous to the C elegans glf-1. Also disclosed is the use of RNAi agents targeting this enzyme to treat or prevent a parasitic nematode infection, and the use of this enzyme to detect a nematode infection.
...

Inhibition of Degradation of Extracellular Matrix

This application realtes to a method of inhibiting the degradation of an extracellular matrix associated with islet beta cells, said method comprising contacting said extracellular matrix with an effective amount of a heparanase inhibitor.
...

Generation of cartilage using magnetizable particles

There is described a method of magnetically manipulating a cell in vivo which comprises the association of a magnetizable particle with a cell. More particularly, there is described a method of magnetically manipulating a cell which comprises the association of a magnetizable particle with a cell characterized in that the method comprises agonizing or antagonizing ion channels within a cell by the association of a magnetizable particle with a cell. There is also described the use of a magnetizab
...

Whitened exine shells

A whitened exine shell haying a degree of whiteness (L*) of greater than 65 measured under D 65 illumination. The shell can be used as a delivery vehicle for an active substance, or as an antioxidant. The invention provides a formulation containing the whitened exine shell together with an active substance; and a method for preparing the shell by isolating an exine shell from a naturally occurring spore under treatment conditions which do not include acetolysis, and treating the isolated shell o
...

Method of obtaining exine shells of narurally occurring spores

A method for obtaining exine shells of naturally occurring spores directly from harvested biological material, the method comprising the steps of (a) treating the material with a mixture of an acid anhydride and an acid catalyst and (b) separating the exine shells from the mixture after treatment.
...

Culture of Crypthecodinium cohnii and microorganisms derived therefrom

Crypthecodinium cohnii, or microorganisms derived from Crypthecodinium cohnii, are grown in a culture medium including propionic acid. The propionic acid increases the production of one or more of dry cell weight, total lipid and docosahexaenoic acid.
...

Acetic Acid and a Buffer

The present invention relates to a composition comprising : a) 0.01-20% wt/wt acetic acid and b) a physiologically tolerable buffer capable of maintaining acetic acid at a pH in the range of 2-7; and use of such a composition as an antimicrobial agent.
...

Compositions and Methods of Use of Standardized Mixtures

The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.
...

Methods and Compositions for Identifying Biomarkers Useful in Characterizing Biological States

The present invention relates to methods, compositions, and kits for identifying biomarkers useful in characterizing biological states. In particular, the invention relates to methods and compositions for molecular characterization of biological states by gene expression profiling. The invention also relates to assessing effects of DNA polymorphisms on regulation of transcription. The biomarkers and polymorphisms identified find use in diagnostic and treatment approaches, e.g., some embodiments
...

Biomarker

Described are gastrointestinal cancer specific biomarkers comprising the nucleic acid sequence of the Engrailed-2 (EN2) gene or the amino acid sequence of the encoded EN2 protein. Also described are uses of the biomarkers in the treatment, diagnosis, monitoring and imaging of gastrointestinal cancer.
...

Biomarker ER2 for Gynaecological Cancer

Described are gynaecological cancer specific biomarkers comprising the nucleic acid sequence of the Engrailed-2 (EN2) gene or the amino acid sequence of the encoded EN2 protein. Also described are uses of the biomarkers in the treatment, diagnosis, monitoring and imaging of gynaecological cancer.
...

Peptidic Conjugates Comprising Polyhistidine Sequence and Free Cysteine and their Uses in Imaging

Bioconjugates for use in imaging are disclosed include a linker sequence fused to a polypeptide capable of binding a target in a biological system. The linker sequence is designed so that it is capable of being radiolabelled, e.g. with a complex comprising a radionuclide, via a free cysteine residue and the polyhistidine sequence in the label that are both capable of simultaneously binding to a complex comprising the radionuclide. These interactions can improve significantly the rate and efffici
...

Micellar Compositions for Use in Biological Applications

This invention relates to a composition comprising a micelle for use in biological applications, the micelle comprising: a substantially water-insoluble conjugated polymer which exhibits luminescence or fluorescence from about 300 nm to about 1500 nm of the electromagnetic spectrum: a biocompatible surfactant and/or lipid: and a MRI active agent.
...

Electrically Conductive Dna Polymer Hybrid and Method of Manufacture

An electrically conductive DNA polymer hybrid, in particular a DNA polymer hybrid nanowire comprising a polymer having a first functional group that is complementary to a second functional group on a molecule, wherein the said functional groups arc reactable by a coupling reaction to bind the molecule to the polymer. The invention also relates to a method of manufacturing an electrically conductive DNA polymer hybrid conductive nanowire, a sensing device and an integrated circuit comprising the
...

Markers of Oocyte Viability

There is disclosed a method of determining the potential of individual oocyte to develop into a viable embryo, which method comprises establishing the concentration of any one or more of the markers identified in Table 2 in the follicular fluid of a single follicle from which said oocyte is derived and comparing the concentrations obtained to a reference concentration range that is established by comparing the concentration of said one or more markers from one or more follicles containing a fert
...

Scaffold

The present invention provides a polymeric scaffold containing an antibacterial photoactive drug and optionally comprising seeded cells such as stem cells. The invention also includes methods of using the scaffold for tissue regeneration, prevention or reduction of infection whilst tissue regeneration occurs, methods for improving graft or implant survival, promoting scaffold integration and tissue repair and wound healing.
...

An Agent for Transporting Nuclear Spin Order and for Magnetic Resonance Imaging

An agent for magnetic resonance studies, the agent comprising hyperpolarized 15N labelled N2O in solution or liquid 15N-N2O.
...

Tissue Analysis

The present invention provides a method of tissue analysis, disease modelling or drug development comprising the steps of providing a tissue sample comprising cells of at least two different cell types; exposing cells in the tissue sample to an agent ex vivo; exposing the cells to three or more detection means arranged to distinguish between three or more different cell types and/or different cell states; detecting the detection means in order to determine the different cell types and/or differe
...

A Method of Detecting a Subject At Risk Of, Or Having, an Indication Associated with Coronary Artery Disease

There are disclosed methods and biomarkers for detecting an indication associated with, or risk of, coronary artery disease In an embodiment, a method includes obtaining a sample including bone marrow-derived cells and a growth factor; measuring a ratio of cell count of the cells and concentration of growth factor, and determining, based on the ratio of the cells and the concentration of the growth factor, risk of coronary artery disease In another embodiment, a biomarker includes a ratio of a c
...

Fluidics Apparatus and Fluipics Substrate

A fluidics apparatus is disclosed for manipulation of at least one fluid sample, typically in the form of a droplet. The apparatus has a substrate surface with a sample manipulation zone for location of the fluid sample. A transducer arrangement such as an interdigitated electrode structure on a piezoelectric body provides surface acoustic waves at the substrate surface for manipulation of the fluid sample. The substrate surface has an arrangement of surface acoustic wave scattering elements for
...

Methods Concerning PPAR Delta and Antagonists Thereof

Non-human animals which overexpress PPARd or which express transgenic PPARd are useful as models for inflammatory skin conditions such as psoriasis. Test substances can be screened to assess their suitability for the treatment of inflammatory skin conditions such as psoriasis. Methyl 3-({[2-(methoxy)-4-phenyl]amino}sulfonyl)-2-thiophenecarboxylate can be administered topically for the prevention or treatment of psoriasis.
...

Apparatus and Method for the Detection of Cells

An apparatus and method for in-vivo detection of magnetically labelled biological molecules or cells, located in a flowing medium in a human or animal body such as the blood stream (13). The apparatus provides a magnetic field to a region of interest inside the body in order to attract the magnetically labelled biological molecules or cells (15) to the region of interest. A second magnetic field provides a variable magnetic field to the region of interest and which transfers energy to the magnet
...

Biomolecule Binding Ligands

The invention provides biomolecule binding ligands, collections of biomolecule binding ligands, and their use in the purification of biological mixtures and in the identification of ligands having an affinity for a substance. The ligand is a compound of formula (III) or a compound of formula (IV): wherein for compounds of formula (I) one of R1a, R1b, R2, R3 and R4 is a group comprising a linker attached to a support, and the others of R1a, R1b, R2, R3 and R4 are independently selected from optio
...

Supramolecular Handcuffs in Polymeric Architecture

This invention pertains generally to supramolecular polymers comprising a polymeric molecule linked to a first CB[8] guest molecule and an attachment compound linked to a second CB[8] guest molecule, wherein the first and second CB[8] guest molecules form a ternary host-guest complex with a CB[8] molecule which non-covalently links the polymeric molecule and the attachment compound in a supramolecular polymer. These polymers are useful as vehicles for delivery of a therapeutic compound for use i
...

Composite biomaterial formed by cooling a fluid composition on a porous solid and removing solidified crystals of the liquid carrier

A process for the preparation of a composite biomaterial comprising providing a first solid component comprising one or more of collagen, a glycosaminoglycan, albumin, hyaluronan, chitosan, and synthetic polypeptides comprising a portion of the polypeptide sequence of collagen, and optionally an inorganic material, the component having a surface portion that is porous; providing a fluid composition comprising one or more of collagen, a glycosaminoglycan, albumin, hyaluronan, chitosan, and synthe
...

Protein Aggregation Prediction Systems

We describe methods for identifying aggregation-prone regions in structured-that is folded-proteins. Embodiments of the method use a local propensity for aggregation (Ai) at an amino acid position, this being determined by a combination of a hydrophobicity value, an [alpha]-helix propensity value, a [beta]-sheet propensity value, a charge value and a pattern value for the amino acid position. This is combined with local structural stability values for the amino acid positions to identify one or
...

Agents for Detecting and Imaging Cell Death

This invention relates to molecular imaging agents comprising an S78C mutant synaptotagmin I C2A domain. These agents may be useful in detecting or assessing cell death in vitro and in vivo, for example in tumours following cancer treatment.
...

TRX1 Antibodies

Humanised antibodies, TRX1 include modified constant regions and light and heavy chain framework of human antibody and CDR regions derived from a mouse monoclonal antibody. TRX1 binds to an epitope of a CD4 antigen on CD4 positive human T-cells or monocytes. The antibodies are designed such that they may or may not include (i) CDRs that are free from glycosylation sites or (ii) do not bind the Fc region of the receptor. Also claimed is a method of treating a graft transplant patient by administe
...

Galvanic micro-pump e.g. for generating small micro-flows in bio-technology, uses partitioning wall with high resistance for convective flow of electrolyte fluid

A galvanic micro pump has two electrodes (1,2) and at least one working chamber containing an electro-liquid and with each working chamber joined via an ion-conductive partitioning wall to a second chamber (6) which is provided with a DC voltage source, and a connection of the working chamber (8) and the second chamber (6) exists with a DC voltage source. The partitioning wall (4,5) has a high resistance for convective flow of the electrolyte fluid touching it and on the basis of the choice of e
...

Material layer and electro-surgery system for electrosurgical tissue fusion...

The invention relates to a biocompatible material layer for use as an intermediate layer between tissue surfaces to be fused with a fusion of body tissue. The layer of material is such that it causes the body to be fused between tissue surfaces areas of relatively higher electrical resistance and areas of relatively lower electrical resistance.
...

Cell Separation Device, Cell Separation System and Cell Separation Method

A cell separation device which can perform a continuous processing without bonding fluorescent molecules or magnetic particles to the surface of the cell membrane, a cell separation system, and a cell separation method, wherein when a sample cell suspension containing the desired target cells is supplied continuously from a sample inlet and physiological saline is supplied continuously from a physiological saline inlet, the sample cell suspension flows together with the physiological saline in a
...

The reduction of redox-sensitive substances by bacteria of the genus Alteromonas or Shewanella

Bio-treatment of aqueous effluents, in particular to remove colour therefrom, is carried out in a Continuous Oscillatory-flow Baffle Reactor. Redox-sensitive substances are reduced by means of bacteria of the genus Alteromonas or Shewanella, or other facultative anaerobes, in a reaction under substantially anaerobic conditions. The redox-sensitive substances that may be reduced include heavy metals, radionuclides, and organic dyes, in particular azo dyes. An electron mediator is introduced, whic
...

Automated Vitrification System

Market Opportunity Cryopreservation of biological materials by vitrification has made significant inroads in displacing the more conventional slow-freeze process. However, technicians must conduct a laboriously manual and technique-driven process to vitrify biological materials. Manual vitrification is a non-scalable low-throughput process. Vitrification is practiced on small specimen volumes, (∼1 μL), limiting its application to discrete cells such as oocytes, embryos, and blastocysts
...

Laplace Pressure Trop for Microfluidic Droplet Formation from Asynchronous Sources and Different Inlets

Tech ID: 21232 Brief Description Researchers have developed a Laplace pressure trap that can fuse droplets from different inlets and fuse droplets generated at different frequencies. The device traps and fuses droplets passively by balancing the driving hydrostatic pressure with increasing Laplace pressure imposed by the device’s design geometry. Video frames showing the Laplace pressure trap and of a single droplet fusion event at the Laplace trap. * Frame A - Reference droplet can
...

Device for High Efficiency Cell Encapsulation using Novel On-Demand Droplet Generation and Impedance-Based Detection

Tech ID: 21236 Brief Description Researchers have developed a novel microfluidic device that is capable of encapsulating cells at a very high efficiency. The device integrates impedance measurement with a novel on-demand droplet generation process to enable the selective generation of droplets that contain encapsulated cells only when a cell is present. This ensures that a high percentage of cells are encapsulated rather than droplets that do not contain cells. The device consists of two
...

Colloidal Self-Assembly of Droplets for High Density Microfluidic Micro-Reactor Arrays with High Throughput Functionality

Tech ID: 21274 Brief Description Researchers have developed a simple method for the rapid self-assembly of predictable high density droplet-reactor arrays for high throughput microfluidic applications in biology and chemistry. By controlling the ratio of the chamber height to droplet diameter, the resulting self-assembled 3D colloidal, lattice droplet pattern formations can be selectively tuned for optimal real-time and/or long-term 2D visualization and image capture of reactions occuring in
...

Microfluidic Device using Dielectrophoresis Separation of Heterogeneous Cell Populations

Tech ID: 21272 / UC Case 2011-181-0 Brief Description Researchers have developed an automated microfluidic device that traps different cell populations in different chambers based on the cells’ dielectric properties. The device consists of one main channel with individual sets of electrodes in three or more different chambers. Each set of electrodes generates a non-uniform electric field that traps and therefore separates a heterogeneous cell population at different frequency ranges due to
...

Facile Method for Modifying Retrovirus Envelopes to Purify Retroviruses and/or to Enhance Gene Delivers

Tech ID: 21217 Brief Description Researchers have developed a novel and convenient method to molecularly tune the exterior surface of retroviruses. The glycoproteins on the surface of viruses and virus-producing cells were activated via oxidation and were further bioorthogonally conjugated with desired functional molecules. These modified retroviruses were able to transduce cells at a high efficiency. Full Description By using the method described, the retroviral envelope was shown to
...

High Strength Nanotechnology-based Inorganic Binder

A technology platform that permits the creation of inorganic solids with very high compressive strength, which permits the creation of light weight, fire resistant, mineral foam composites. These structural mineral foam products can be used to form fire resistant insulation, rigid foam products, casting mixes and also create structural insulated panels. An alternative formation can utilize waste agri-fiber to create fire resistant composite panels - which have demonstrated superior fire resi
...

Chitosan Green Tea Polyphenol Complex for Wound Healing

In recent years, oxidative stress has been implicated in a variety of degenerative process and diseases, including acute and chronic inflammatory conditions such as wound healing. Green tea polyphenols have shown anti-oxidant property. The present study discussed the application of chitosan green tea polyphenol complex on the wound healing.In my research, chitosan polyphenol complex has enhanced the healing of incision wounds by increasing the breaking strength of the wounds. In excision wound
...

Label-Free Protein Detection

Background Conventional methods to detect the presence or quantity of specific proteins or other molecules in biological samples rely on the use of target-specific probes such as antibodies, which can be detected via chemical tags or fluorescent labels. However, conjugating labels to proteins can alter the functional properties of a probe and thus a number of label-free methods to detect proteins such as the use of functionalized silicon nano-wires or carbon nano-tubes have been developed. How
...

Superior Liquid Uptake Resins for Polymer-supported Synthesis (SLURPS)

Background Solid phase synthesis, or polymer-supported synthesis, is a technique for chemical transformations in which molecules are bound to a solid, such as a polymer bead, during the reaction and hence are easier to separate afterwards. The main benefit lies in the fact that these supports, by being insoluble, allow the easy separation of bound product from soluble reagents and contaminants. When used to support catalysts (including enzymes), they provide a means of recovering and recycl
...

Cucumber Mosaic Virus Inducible Viral Amplicon (CMVIVA) Expression System

Tech ID: 11438 / UC Case 2006-640-0 Abstract A Chemically Inducible Cucumber Mosaic Virus Amplicon Expression System for Production of Recombinant Proteins in Plant-Based Systems Full Description Plant cell cultures and transgenic plants have been developing as a potential platform for production of recombinant proteins, particularly playing an increasingly important role in production of human therapeutics. However, efficient expression systems that can be tightly regulated are curre
...

Cost-Efficient, Biodegradable Microspheres with Control of Diameter, Density, Color and Surface Chemistry

BACKGROUND: Currently commercialized microspheres for flow measurement and bioscience applications are manufactured via polymerization processes that are costly or impossible to customize and require handling of hazardous materials. Further, the resulting microspheres contain hazardous or non-biodegradable components which often prohibit their release to the public environment. These issues drive manufacturing cost up and limit the size of markets for the product. DESCRIPTION: Researchers at
...

A New Method to Enhance Nerve Growth

BACKGROUND: Stroke and CNS injuries together cost Americans an estimated $75 billion a year in medical and rehabilitation expenses and lost productivity. Currently, there are no effective, clinically approved methods that promote nerve regeneration and subsequent restoration of CNS function. New research from the University of California has identified a potential therapeutic target for modulating neuron outgrowth. An antigen, present on most cells but long thought to be absent from neurona
...

Method to Diagnose Giant Cell Arteritis

BACKGROUND: Giant cell arteritis is an inflammatory disease that primarily affects arteries in the head and neck. In the U.S. alone, it is estimated that 200 per 100,000 people over the age of 50 develop giant cell arteritis. If not diagnosed and treated immediately, this disease can rapidly cause blindness. Currently, the most definitive method of diagnosis is biopsy of the temporal artery. This method, however, is not only undesirable due to its invasive nature, but is also not completely reli
...

End-Specific Antibody to Detect Apoptosis

BACKGROUND: Programmed cell death or apoptosis is a critical event in normal cellular differentiation and development as well as in degenerative diseases, cancer and aging. Currently, the most widely used assay for detecting apoptosis is DNA fragmentation. However, since DNA fragmentation can occur in a variety of situations without apoptosis, is a late stage nuclear event in apoptosis, and increases with postmortem time, it is not a reliable indicator of apoptosis. DESCRIPTION: Researchers
...

Expression and Purification of ATM Protein using Vaccinia Virus

BACKGROUND: Ataxia telangiectasia (AT) is a genetic recessive disorder involving a large range of symptoms including telangiectasia (dilation of blood vessels) on the eyes, face, and shoulders, ataxia (loss of balance), cerebellar degeneration, radiosensitivity, cancer predisposition, immunodeficiency and premature aging. At a cellular level, AT cells display cell cycle checkpoint defects, chromosomal instability and sensitivity to ionizing radiation. The protein mutated in AT, ATM (Ataxia Tela
...

New Factor involved in Lipid Disorders

BACKGROUND: Fat cells, or adipocytes, are a specialized cell type that synthesize and store fat (triglycerides) in periods of nutritional abundance, and hydrolyze these fats when needed to meet demand for energy. The size and distribution of adipose tissue stores in humans and animals clearly influences metabolism and development of diseases including obesity and diabetes. Most of the development of mature adipocytes from precursor cells, a process known as differentiation, occurs shortly befor
...

Diagnostic Test for Mutations in the AT Gene

BACKGROUND: Ataxia telangiectasia (AT) is a genetic recessive disorder involving a large range of symptoms including telangiectasia (dilation of blood vessels) on the eyes, face, and shoulders, ataxia (loss of balance), cerebellar degeneration, radiosensitivity, cancer predisposition, immunodeficiency and premature aging. At a cellular level, AT cells display cell cycle checkpoint defects, chromosomal instability and sensitivity to ionizing radiation. The protein mutated in AT is ATM (Ataxia Te
...

Technique for Expressing any RNA in Curcular Form

Antisense mRNA and ribozymes (catalytic RNA) have great potential for controlling gene expression. A major road block remains: many linear anti-sense or ribozyme RNAs are unstable when expressed in living cells. Due to this instability, these RNAs do not accumulate enough to provide an adequate level of inhibition for practical control of gene expression. The most promising method to stabilize RNA transcripts involves making them circular. Existing methods of circularizing RNA either have lo
...

Glucanase Regulation of Plant Growth and Development

Hydrolysis of the polysaccharides that compose plant cell walls is critical to cell expansion, and is therefore a key process in governing the growth and development of plants. Specifically, beta-glucan polymers form a ubiquitous matrix in plant cell walls, forming a molecular fabric around cells that must be loosened and broken down before a cell can expand. Since there is a dynamic balance between beta-glucan polymerization and depolymerization, subtle changes in the glucanase enzymes that cat
...

Commercial Auxin Substitute

A University of California researcher has identified a low-cost, non-toxic compound that can be exogenously applied to plants as foliar sprays to annual vegetable and perennial tree crops under commercial production to elicit an auxin response. Currently, various auxin derivatives are used for this purpose (notably 2,4-D), but they are mutagenic and carcinogenic, which has made the use of such compounds in agricultural applications unacceptable to consumers in a number of European and Pacific-ri
...

Multiplexing Protein Binding Microarrays for Genetic Analysis

BACKGROUND: Our ability to understand and practically benefit from the control logic encoded in the human genome is currently limited by a lack of accurate information regarding promoter regulation. Gene regulatory proteins play critical roles in converting the genome into the complex ensemble of expressed genes which define variations in cell types and human health. Currently available antibody and surface Plasmon resonance-based methods have provided unprecedented insights into the promoter-sp
...

Safe and Potent Synthetic Lung Surfactant for Treating RDS and Acute Asthma

BACKGROUND: The use of lung surfactant dispersions, based on lipid and protein components extracted from animal lung tissue, has significantly reduced mortality and morbidity from respiratory distress syndrome (RDS) in premature infants in the past decade. However, existing lung surfactants are not potent enough or cost effective enough to treat adult RDS and acute asthma, both of which represent far larger indications than neonatal RDS. Growing concerns of possible viral and prion contamination
...

Enzyme for Antimicrobial and Vaccine Development

Scientists at the University of California have shown that a specific enzyme is an essential regulator of Salmonella virulence. This enzyme is prevalent in many organisms including most enterobacteria, certain gram negative and gram positive bacteria, as well as archaebacteria. It is expected that this enzyme will be a major virulence factor for many types of infection, including cholera (Vibrio cholerae), the plague (Yersinia), brain infections (Haemophilus influenzae), typhoid fever (Salmonell
...

Method and Probes for the Identification of Microbial Genes specifically induced during Host Infection

BACKGROUND: Pathogenic microbes express certain virulence properties only during the infection of a host. Attempts to identify these virulence factors and to develop methods to control infections by these pathogens have been limited by the inability of researchers to accurately mimic the in vivo conditions of the host cell in vitro. A new method, IVET (in vivo expression technology), has recently provided researchers with the means for discovering the genetic source of such virulence factors in
...

Novel Antisense Agent

University of California scientists have synthesized novel oligonucleotide analogs for use in antisense and related applications. Unlike ordinary DNA and RNA strands, the U.C. analogs incorporate modifications to the sugar-phosphate backbone that alter electrostatic properties of the strand. These changes enable U.C. analogs to resist attack by nucleases, restriction enzymes, and topoisomerases, thus making the analog much more stable in vivo than existing antisense agents. Also, these changes e
...

Inexpensive, Sensitive, Rapid and Selective Detection of GHB (Gamma-Hydroxybutyrate)

Researchers at the University of California have invented a rapid, selective, sensitive, and inexpensive enzyme-based colorimetric assay for GHB that will provide means by which: * People can protect themselves from unknowingly ingesting GHB; * Law enforcement can obtain probable cause to detain persons suspected of possessing GHB; * Forensic laboratories can screen suspect samples before definitive testing; * Clinical researchers can inexpensively monitor GHB levels in bodily fluids inclu
...

Use of Vanadium Bromoperoxidase as a Signal-Generating Enzyme for Chemiluminescent Systems: Test Kits and Analytical Methods

PATENT ABSTRACT: Aqueous compositions, test kits and methods can be used to detect hydrogen peroxide or vanadium bromoperoxidase by generating a chemiluminescent signal in the presence of the analyte. Signal generation as well as reaction kinetics are improved by using a composition which comprises a 2,3-dihydro-1,4-phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing or pseudohalogen-providing source; and a peroxide or peroxide-generating reagent composition. REFERENCE
...

Tough, Self-Healing Silicone Materials

Siloxane- and organosiloxane-based materials (collectively termed "silicones") are ubiquitous in commercially available goods and high technology applications, often in the form of oils, rubbers, resins, and hard solids. Their general usefulness is due to their broad and tunable range of beneficial properties, such as their low dielectric constant, high thermomechanical stability, high biocompatibility, high optical transparency, and controllable hardness, porosity, and hydrophilicity. Moreover,
...

Initiators for Block-Copolypeptide Synthesis

BACKGROUND: Amino acid-N-carboxyanhydride (NCA) polymerizations employing conventional initiators (e.g. hexylamine or sodium methoxide) are plagued by chain-breaking transfer and termination reactions which prevent the formation of block copolymers. The mechanisms of NCA polymerization have been under intensive study to find a method for enhancing control over chain growth in these reactions. These investigations have been severely hindered by the complexity of the polymerizations, which can pro
...

Moisture-Resistant Adhesive Polypeptides

BACKGROUND: Surgical tissue adhesives provide an alternative to suturing, packing, or stapling planes of tissue together. Use of adhesives for wound closure is desirable since adhesives can be very fast acting and assure complete closure. Marine adhesives (such as those used by barnacles) can function over wide temperature ranges, fluctuating salinities, humidities, and under strong currents. These glues are able to rapidly form permanent bonds to a wide variety of substances with complex and of
...

Edible Coating for Crispy Texture in Processed Food

University of California researchers have discovered that a sweet-corn extract can be used to modify the texture of processed foods to improve their crispiness. The extract may be applied as an edible coating or added directly to the food item prior to processing. After application of the extract, the item may be fried, toasted, dried, baked, and soaked while retaining the desired textural properties. This invention will be particularly useful in coating fried foods, where they remain crispie
...

Recombinant Prolactin Antagonist

The hormone prolactin (PRL) exerts various effects in a wide range of physiological processes. Research suggests that PRL may promote cellular proliferation in both breast cancer and prostate cancer cells. University of California researchers have developed a modified PRL analog (S179D) that acts as a strong antagonist to PRL in cell proliferation assays. S179D consists of a wild-type PRL with a single amino acid substitution. This substitution alters the activity of the molecule such that it
...

Identification of Sortase

BACKGROUND: Hospital-borne bacterial infections present a major challenge in patient care due to the rising number of strains resistant to multiple antibiotics in recent years. Gram-positive bacteria, such as Staphylococci, Streptococci and Pneumococci, are the most common cause of these often fatal infections and are particularly more difficult to treat in immunocompromised patients. Therefore, there is an urgent need for identifying novel targets sites in these pathogens for the development of
...

Identification of Sortase

BACKGROUND: Hospital-borne bacterial infections present a major challenge in patient care due to the rising number of strains resistant to multiple antibiotics in recent years. Gram-positive bacteria, such as Staphylococci, Streptococci and Pneumococci, are the most common cause of these often fatal infections and are particularly more difficult to treat in immunocompromised patients. Therefore, there is an urgent need for identifying novel targets sites in these pathogens for the development of
...

Detection of ATM Mutations and Polymorphisms with MEGA-SSCP

BACKGROUND: Ataxia telangiectasia is an autosomal recessive disorder characterized by progressive cerebellar degeneration, immunodeficiency, growth retardation, premature aging, chromosomal instability, acute sensitivity to ionizing radiation, and a predisposition to cancer, particularly breast cancer. It is caused by mutations in the ATM gene which lead to defects in the DNA repair process and cell cycle control. Given the severity of the disease, there is a need for efficient and accurate diag
...

Phosphorylation of Histone H2B as a Marker for Cell Proliferation and Differentiation in Development and Disease

BACKGROUND: Determining the cell cycle state of human cells can provide an important method for early detection of cancerous growth, especially the detection of cells at the G2/M phase of beginning cell division, or mitosis. However, current cell cycle detection methods cannot be correlated unambiguously with mitosis. Many cell cycle regulators are expressed during the entire cell cycle and are activated posttranslationally during cell division by modifications such as phosphorylation, so antibo
...

Chemokine and Peptide Derivatives for Would-Healing, Fibrotic Disease Treatment, and Tumor Supression

A University of California researcher has characterized an avian chemokine that acts both directly and as an agent for various growth factors in wound-healing. The gene that encodes this protein is an inducible chemokine gene that is expressed at low levels in tissue of mesenchymal origin, but is expressed at high levels shortly after wounding and persists throughout the period of granulation tissue formation. It is also highly expressed in tissue surrounding tumors induced by Rous-sarcoma virus
...

Vectors for the Recombinant Expression of Human Immunoglobulins

Monoclonal antibodies (mAbs) are an essential tool in numerous research, therapeutic, and diagnostic applications, as mAbs can be customized to bind a desired antigen. While this is highly advantageous in generating very high binding specificity, the actual process of producing mAb-producing hybridomas is difficult and time-consuming, and involves mAb protein chains (immunoglobins) derived from animal sources that are not always suitable for use in humans. Researchers at the University of Cal
...

Monoclonal Antibodies and Method for Detecting Dioxins and Dibenzofurans

Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are toxic pollutants which pose persistent threats to both the human population and to the biosphere in general. Although the risk posed by these contaminants has ultimately come to be appreciated, there has been no economically viable detection system suitable for either identifying the sites of pollution or the populations at risk. An obvious need exists. Exploiting modern techniques of molecular biology and i
...

Polypeptides and Antibodies Associated with HTLV

BACKGROUND: Human T-lymphotropic viruses (HTLV) are part of a family of retroviruses called oncogenic retrovirues. HTLVs were originally identified for their ability to infect human lymphocytes, particularly T cells, resulting in their transformation to lymphomas and leukemias. More recently, they have been associated with "autoimmune-like" neurological and rheumatic disorders, such as Tropical Spastic Paraparesis. HTLVs have a highly conserved X region, containing five overlapping open reading
...

Tissue-Specific Gene Inactivation of Beta-1 Integrin

BACKGROUND: Beta-1 integrin is a critical member of the large family of integrin proteins necessary for cell-extracellular matrix adhesion and bi-directional signaling across the cell membrane. Conventional deletion of beta-1 integrin from the mouse genome results in embryonic death soon after implantation of the blastocyst. Thus, analysis of beta-1 integrin function beyond post-implantation embryogenesis is not possible in a classic deletion model. DESCRIPTION: Researchers at the University
...

High-Speed, High-Resolution Impedance Sensor

BACKGROUND: While there has been extensive work in developing radio-frequency probes for measuring proximal impedances in air, these probes have been limited to applications using good conductors as their proximal impedance source. For electrolytic and biological samples, lower frequency probes have generally been employed, but they are limited to a relatively low signal-to-noise ratio and slow signal response due to the stray capacitances and large resistances often encountered in such samples.
...

Suspended Carbon Nanotubes as Mulecular Sensors

BACKGROUND: Relatively large surfaces areas and great sensitivity to environmental conditions make carbon nanotubes (CNTs) very promising candidates for sensing elements. However, all existing CNT sensing methods based on electronic or acoustic techniques require complicated nanotube architectures involving multiple fabrication steps. Such complexity can increase the probability of material defects, and often requires CNTs of very high purity. Such problems have blocked the commercialization of
...

Platform for Chemical and Biological Sensing by Surface Enhanced Raman Spectroscopy

BACKGROUND: Surface-Enhanced Raman Spectroscopy (SERS) is an optical analysis technique that can provide molecular identification and quantification by recording a spectrum that displays characteristic vibrational fingerprints of molecules or parts of molecules. SERS is potentially among the most sensitive analysis techniques with molecular identification capabilities and has the benefit of extraordinary sensitivity. In the past, however, SERS platforms have been highly technical preparations.
...

Hybrid Energy Transfer for Nucleic Acid Detection

BACKGROUND: The need for efficient assays to detect DNA sequences is enormous and constantly growing. The recent increases in the number of sequenced genomes for bacterial and viral pathogens as well as humans provide the potential for improved medicine and diagnostics. Molecular beacon-based nucleic acid detection platforms are actively being pursued in basic research and are now emerging as the basis of powerful new sensing and diagnostic methods. However, optical detection requires high beaco
...

Electronic Detection of Molecular Targets, Including Proteins, Oligonucleotides and other small Molecules

BACKGROUND: While many assays exist for the detection of DNA, RNA, proteins and other molecular targets, most sensors require sample purity and rigorous controls available only under ideal laboratory settings. These constraints significantly dampen the effectiveness of most reported sensing technologies for real world applications. Rapid, accurate and cost-effective sensors that can quickly identify and quantify targets within contaminated samples would provide a critical tool for diagnostics,
...

One-Step Manufacture of Nanowire Biosensors

Electrochemical biosensors based on nanotubes or nanowires could be widely used in diagnostic, research, and security applications. However, the problems associated with functionalizing and placing these nano-elements on a device have prevented the practical application of this technology. University of California scientists have developed a simple one-step methodology for the synthesis of functionalized nanowires that act as biosensors. Nanowires of 100 to 200 nm have been tested that can ac
...

Tunable, Full-Color Electroluminescent Array

BACKGROUND: A light-emitting diode (LED) is a semiconductor device that emits visible light when an electric current passes through it. Traditionally, LEDs were made with inorganic materials. However, inorganic semiconductor LEDs lack ease of processibility on large and flexible substrates. In the last decade, Organic LEDs (OLEDs) have become very popular with their diversity, easy processibility and possibility of fabrication on flexible and large area displays. But organic LEDs suffer from fu
...

Oxide-Supported Metal Nanoparticle Catalyst

BACKGROUND: Gold nanoparticles, particularly with dimensions less than 10 nm, exhibit unexpectedly high catalytic activities toward different types of reactions, a property not revealed in bulk gold. In order to obtain high catalytic activity, gold nanoparticles are generally dispersed on support materials such as oxides. The overall performance of a supported gold nanoparticle catalyst greatly depends on the size and shape of the gold nanoparticles, the structure and properties of oxide suppor
...

Novel Supramacromolecular Polymer Complexes Providing Controlled Nitric Oxide Release for Healing Wounds

Technology To overcome these deficiencies, UofToronto researchers have developed a novel, stable and biocompatible system for generating a durable release of NO. This technology is based on the discovery that a supramacromolecular complex, based on interacting GSNO conjugated to 2 polymers through intermolecular hydrogen bonding, can stabilize the conjugated GSNO and provide a controlled release of NO for an extended duration, not achievable by any currently available system. Backg
...

Nano-Composite Polymeric Membrane System for Drug Delivery

The Background Then human body has its own homeostatic mechanisms to regulate a variety of physiologic parameters within a certain range. In instances of disease, however, these parameters may be affected due to various mechanisms. Regulated drug delivery is a concept in which drugs are delivered at an appropriate rate in response to stimuli. Disease states may cause a change in some parameters of the body and this can be used as a stimuli or trigger for the onset and offset of the
...

Readily Available Unprotected Amino Aldehydes

The Technology A new class of compounds, containing two functional groups, an aldehyde and an unprotected amine has been developed. This finding has solved a significant problem in complex amine chemistry. Construction of complex biologically active molecules is now feasible with minimal use of protecting group manipulations. These novel molecules contain both a secondary amine functionality and an aldehyde functionality that are stable towards condensation. Accordingly, t
...

A Novel Proangiogenic Compound

Background Angiogenesis, the formation of new blood vessels from a preexisting vascular bed, plays a critical role in tissue growth and repair. Angiogenesis is regulated by a complex collaboration between endothelial and mesenchymal cells and the intricate signaling between multiple angiogenic growth factors. The Angiopoietins, a cluster of angiogenic growth factors, are ligands to Tek/Tie2, a receptor tyrosine kinase that is highly expressed in endothelial cells. Converging lines o
...

Newly Described Peptide with Actin Stabilizing Activity

The Background The cellular cytoskeleton functions to maintain the spatial shape and volume of mammalian cells; it is crucial for functions such as locomotion (migration), endocytosis, and phagocytosis. Actin is one of the key proteins of the cytoskeleton, and the physiologic assembly and disassembly of actin filaments in response to regulatory signals maintains homeostasis of cellular functions and responds to external stress to protect the cell. Cells move by remodeling actin filaments at t
...

Thermal Dependent Injectable Polymer Matrix

Technology The technology is a novel drug delivery polymer matrix comprising an inverse thermal gelling polymer with an anionic polymer. This polymer matrix is faster gelling than each of the separate components and it provides a drug delivery vehicle for many applications. The anionic polymers interact highly with the solvent, thereby decreasing the gelation temperature of the inverse thermal gelling polymer. This results in a more mechanically stable gel that is injectable due to the sh
...

PoLi Implant System for Targeted and Controlled Drug Delivery

Technology The PoLi Implant Systems have several advantages over other synthetic polymer drug delivery systems. They are made of natural, fully biocompatible and biodegradable materials. The components are physically crosslinked, thus preventing the need for toxic chemical crosslinking usually required in the preparation of polymeric matrices. All components and degradation products are G RAS. The implants are resistant to a host’s immunological response, thereby preventing encapsulatio
...

Canine Embryonic Stem Cells

Background The establishment of embryonic stem (ES) cell lines has brought great promise and opportunities for regenerative medicine and pharmaceutical research. Embryonic stem cells are derived from embryonic sources and are pluripotent i.e. they possess the capability of developing into a wide variety of different cell types, and tissues and organs. Procedures for the development of embryonic stem cell lines for different species poses challenges due to differences in the pattern of embr
...

Unique Treatment for Type 2 Diabetes and Islet Cell Transplantation

Background Deposition of islet amyloid polypeptide (IAPP or amylin) fibrils is associated with over 90% of cases of type 2 diabetes, and is present regardless of the obesity of the patient. 1 As with other amyloid disorders such as Alzheimer’s disease, IAPP aggregates and fibrils are cytotoxic and are considered to be a significant contributor to β-cell loss and the impairment of islet function which in turn prevents the release of insulin in response to high glucose levels. R
...

Bioreactor Suite ()

Abstract: A major drawback of conventional reactors has been gravity-induced sedimentation of the microcarrier beads. As cell density becomes greater, the beads require more stirring, resulting in high shear levels. Batch cultures are limited to the number of cells that can be grown and maintained. Fixed matrix reactor vessels do not allow for homogenous distribution of nutrients or control of the sheer regimes. But the microgravity bioreactor can grow the cells in 3-D spatial freedom while cau
...

Method for Inhibiting, Arresting and Possibly Reversing Non-enzymatic Glycation

Non-enzymatic glycation and cross-linking (Maillard reaction) are well known processes which accelerate the aging of key biological molecules. For example, non-enzymatic glycation of macromolecules such as proteins and certain phospholipids (e.g., phosphatidylethanolamine and phosphatidylserine) appears to play an important role in the evolution of diabetic complications. Moreover, glycation is involved in aging of structural proteins such as collagen andelastin and in food spoilage. The first
...

Method for Inhibiting, Arresting and Possibly Reversing Non-enzymatic Glycation in Diabetic Patients

Non-enzymatic glycation and cross-linking (Maillard reaction) are well known processes that accelerate the aging of intracellular proteins and extracellular structural proteins such as collagen and elastin. Numerous studies indicate that non-enzymatic glycation appears to play an important role in the evolution of diabetic complications. Consequently, preventing, arresting or reversing this process in diabetics would of great significance. The first two intermediates formed in the Maill
...

Compositions and Methods for Regulating Rna Translation Via CD154 Ca-dinucleotide Repeat

The expression of CD154 (CD40 ligand), a member of the Tumor Necrosis Factor gene family, by activated T lymphocytes is critical in the development of humoral and cell-mediated immunity. CD154 blockade retards the development and progression of immune responses in an array of transplantation and autoimmune disease models ranging from Systemic Lupus Erythematosus to Rheumatoid Arthritis to Multiple Sclerosis. Overexpression of splice isoforms of polypyrimidine tract binding protein differentially
...

method for IDENTIFYING AGENTS WHICH MODULATE gtpASE ACTIVITY INVOLVED IN INSULIN-STIMULATED glut4 TRANSLOCATION

Insulin treatment of fat and muscle cells causes a rapid increase in glucose transport. The basis for this effect is an increase of glucose transporters of the GLUT4 type at the cell surface. This translocation of GLUT4 is achieved by modification of AS160 (Akt substrate of 160-kDa) which has a GTPase activating protein (GAP) domain for members of the Rab G protein family. AS160 is phosphorylated by the insulin-activated protein kinase Akt, Evidence indicates that phosphorylation of AS160 inhibi
...

Regulatory T Cell MEDIATOR PROTEINS and uses thereof

Regulatory T cells (Treg) play an important role in constant and inducible control of T cells responses in vitro and in vivo. Treg cell depletion results in the induction of autoimmune pathology and exacerbation of T cells responses to foreign and self-antigens, including heightened anti-tumor responses as well as ablation of transplantation tolerance and rapid graft rejection. Dartmouth researchers have now found a novel member of the PD-L1 family expressed by Treg cells. This novel pro
...

Nucleic acid sequences encoding luciferase for expression in filamentous fungi

Bioluminescence is the light produced in certain organisms as a result of luciferase-mediated oxidation reactions. The luciferase genes, e.g., the genes from luminous beetle and, in particular the luciferase from Photinus pyralis, are very useful luminescent reporter genes. Despite its utility as a reporter, native luciferase is not generally useful in many organisms, including the filamentous fungi, because of low expression levels. Dartmouth researchers in collaboration with their coll
...

Compositions and Methods for Inhibiting the Synthesis Or Expression of MMP-1 for Treating, Preventing, Or Managing

Overexpression of MMP-1 is associated with several pathological conditions, including the irreversible degradation of cartilage, tendon, and bone in arthritis and the degradation of collagens I and III in tumor invasion and metastasis. Patients with tumors that express MMP-1 have an overall poorer prognosis than patients with tumors that do not express this protein. Dartmouth researchers have now found that the expression of a MMP-1-specific short interfering RNA molecule in a breast can
...

Compositions and Methods for Preventing and Treating Cancer Via Modulating UBE1l And/or ISG15

Individuals with acute promyelocytic leukemia (APL)(FAB M3) express oncogenic promyelocytic leukemia (PML)/retinoic acid receptora (RARa). All-trans-retinoic acid (RA) treatment causes complete remission of APL through induction of leukemic cell differentiation. A hallmark of the RA response in APL is PML/RARa degradation that reverses PML/RARa oncogenic effects. Proteasomal inhibitors prevent PML/RARa proteolysis, despite RA treatment, which is indicative of a proteasome-dependent pathway in th
...

Novel Immunotherapy for Ovarian Cancer

Dartmouth investigators and their colleagues at the University of Kansas have discovered a novel method for rescuing cytotoxic T lymphocytes from destruction by ovarian carcinoma cells in vitro using an animal model system. Ovarian cancer causes more deaths than all other gynecologic cancers combined. Approximately 19,000 new cases of ovarian cancer are reported and 12,000 deaths occur annually. Ninety percent of ovarian cancers are ovarian carcinoma, i.e. epithelial cell-derived. Ova
...

A Reliable, Non-radioactive Method for Monitoring Accumulation of Cholesterol Inside Mammalian Cells After Loading with Low Density Lipoprotein (ldl)

Agents identified as cholesterol inhibitors are useful in preventing and treating cardiovascular and neurodegenerative diseases associated with over accumulation of cholesterol in cells. These agents are expected to slow the development of atherosclerosis and the accumulation of amyloid beta-peptides in the brain, thereby slowing down the symptoms of Alzheimer’s disease. Therefore, methods for detecting and monitoring cellular cholesterol is of critical importance to human health. Dar
...

Identification and Uses of a Novel Transcriptional Activator That Uses Visible Light As Its Inducer

Dartmouth researchers are contributing to the rapidly emerging field of regulatable gene expression. Recent research at Dartmouth has identified a protein complex that activates gene expression in response to light. This may provide a means to induce gene expression using the reliable, readily available, inexpensive, non-toxic, and non-pleiotropic inducer, light. Current methods of controlling gene expression such as inducible promoters, heat shock, hormones, or drugs such as tetracycli
...

Use of Monocyte Chemoattractant Protein-1 (MCP-1) for Treatment of Neuropathic Pain

Chronic pain is a dominant health problem. It is the most common reason to seek a physician's care with nearly 40 million office visits scheduled annually. Pain costs the U.S over $100 billion each year in health care costs and lost productivity (NIH Guide, Vol. 24, 1995). Chronic pain is the most difficult pain to treat, especially chronic pain that follows nerve injury, known as neuropathic pain. These neuropathic pain syndromes include deafferentation pain, diabetic, cancer and ischemic ne
...

Novel Method of Monitoring Angiogenesis

Angiogenesis is the formation of new capillary blood vessels as outgrowths of pre-existing vessels. This tightly regulated process plays a vital role in many physiological processes, such as embryogenesis, wound healing, and menstruation. Angiogenesis is also important in certain pathological events, such as solid tumor growth and metastasis, arthritis, psoriasis, and diabetic retinopathy. Vascular endothelial growth factor (VEGF) is a mitogenic factor that stimulates pro-angiogenic properties
...

Novel Use of Aspirin for Colon Cancer Prevention

The American Cancer Society estimates that colorectal cancer will be diagnosed in 147,000 Americans in 2003 and will cause 57,100 deaths, making it the second-leading cancer killer. A seven-year randomized, double-blind study lead by Dartmouth researchers determined that daily low dose of aspirin can prevent the development of precancerous polyps in patients with an elevated risk for colorectal cancer. The study looked at 1,100 patients with previously diagnosed colorectal adenomas. Som
...

Delineation of a Novel Pathway That Regulates Human CD154 (CD40 Ligand) Gene Expression

The expression of CD154 (CD40 ligand) by activated T lymphocytes plays a central role in humoral and cellular immunity. The fundamental importance of this protein in mounting an immune response has made it an attractive target for immunomodulatory strategies. Several studies have demonstrated that CD154 expression is regulated at the level of mRNA turnover in a manner distinct from other cytokine genes. We report characterization of the cis-trans components of this important regulatory pathwa
...

Methods of Diagnosing, Prognosticating and Treating Collagenase-1 Related Diseases

Degradation of the extracellular matrix is part of the pathological process associated with many diseases: e.g., joint destruction in arthritis, invasion and metastasis in cancer, bone dissolution in periodontitis, and plaque rupture in athererosclerosis. Most of this connective tissue degradation is accomplished by a family of enzymes called the Matrix Metalloproteinases, or MMPs, a family of enzymes that, collectively, degrade most matrix components. A sub-family of MMPs is the interstitial
...

Compositions and Methods for Treating and Identifying Therapeutics for Cystic Fibrosis

Cystic fibrosis (CF) is one of the most common autosomal recessive diseases in the human population. CF as a disease typically involves several different epithelial tissues. However, the principal clinical problem resulting in 90% of all CF deaths is that of defective lung function. This disease is a result of mutations in a single gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. CFTR is a member of the ATP-binding cassette (ABC) family of transmembrane
...

Powerful Research Tool for Screening and Testing of Cholesterol Reducing Compounds

Accumulation of cholesteryl esters as cytoplasmic lipid droplets within cells of human aortic tissue is a characteristic feature of early lesions of atherosclerotic plaque. These cholesteryl esters are produced through biochemical reaction catalyzed by the enzyme acyl coenzyme A:cholesterol acyltransferase (ACAT). In intestines of vertebrate animals, including human, the extent of absorption of dietary cholesterol can be shown to be significantly reduced by inhibiting intestinal ACAT activity.
...

New Treatment for Low Back Pain

Low back pain is a major clinical problem second only to the common cold in its financial and symptomatic impact on human suffering. It has been estimated that 20 percent of the adult population has consulted a heath care provider for low back pain and currently 5 million Americans are partially disabled by low back pain. Therefore, the commercial potential of a novel treatment is quite significant. The study of low back pain has been hindered, until recently by the absence of an animal
...

Identification and Uses of a DNA Digesting Enzyme Potentially Involved in the Pathway of Apoptosis and Cell Death and Useful in Conditions Such As Cystic Fibrosis

Dartmouth researchers are contributing to the rapidly emerging field of apoptosis, which describes a pathway of cell death that is essential for normal human development and that can, when aberrant, lead to many pathological disorders including cancer, neurodegeneration, and auto-immune diseases. Recent research at Dartmouth has identified a protein that is cleaved in human cells undergoing apoptosis. This information leads to a potential marker for apoptosis that might be of value in diagnosti
...

Discovery of a New Class of Antitumor Agents

Dartmouth researchers have synthesized a novel class of antitumor agents. These potential new drugs were designed to bind more tightly to cancer cell DNA than many conventional anticancer drugs by a process called bis-intercalation or "double-binding," much like a molecular staple. Such binding stops the growth of the cancer cell and it dies. Improving upon the concept of bis-intercalation, the Dartmouth chemists have utilized a unique semi-rigid tether connecting the two intercalating
...

Alternative Splicing of the MCI-1 Gene Useful in Cancer Therapy

MCL1 is a member of the BCL2 gene family which is involved in controlling cell viability and immortalization. Because of their ability to keep cells alive, these gene products can contribute to cancer. Recent data demonstrate that, in addition to the full length MCL1 gene product, MCL1 can undergo alternative splicing to a truncated derivative, MCL1s/deltaTM. The shorter alternatively spliced gene product lacks sequences critical to the viability-promoting function of MCL1, but retains a sequ
...

Anti-microbial Protease Inhibitor

Human morbidity and mortality due to bacterial infections has become a problem of great concern due to the well-publicized dramatic increase in the frequency of infections caused by bacteria that are resistant to most, or even all, known available antibiotics. Since even modern state-of-the-art antibiotics can be ineffective for treating disease caused by multi-drug resistant "superbugs" there is an urgent need for the development of new drugs for the treatment of bacterial infections. Researche
...

A Novel Method to Enhance the Activity of Routinely Used Anticancer Agents

Dartmouth researchers have discovered a new methodology that can enhance the activity of routinely used anticancer agents. In particular, this method appears to be selective for tumors that are defective in the p53 tumor suppressor gene, and thereby should spare any additional toxicity to the non-tumor tissue. Much of the past success in cancer therapy has involved the use of cytotoxic anticancer drugs, including many that directly damage the cells DNA (for example, cisplatin, mitomycin C, t
...

Novel High-throughput Screening Assay for Identification of Cholesterol Inhibitors

Dartmouth scholars developed a high-throughput screen comprised of a cell-based assay for identification of chemical inhibitors which block internalization of plasma membrane cholesterol from entering the cell interior. Two novel cell lines originally developed at Dartmouth are used in this screen. Agents identified as cholesterol inhibitors in accordance with this method can block the internalization of plasma membrane cholesterol from entering the cell interior thereby causing cholest
...

Methods for Immunization Or Treatment of Humans and Animals Against Viruses (HIV Including), Infectious Agents, Cancer and Other Genetically Treatable Maladies

Apicomplexan parasites are significant pathogens of humans and animals, and cause diseases such as toxoplasmosis, malaria, cryptosporidiosis, theileriosis, and many others. For these reasons various pharmaceutical companies are in the process of developing specific chemical reagents to inhibit these parasites, or various formulations which may confer immunity to these pathogens. While the de novo pyrimidine biosynthetic pathway is believed to be essential for all apicomplexan parasites this
...

Microwave Powered Sterile Access Port (MSC-22802)

Abstract: This technology was developed to provide a less expensive, easy-to-use method for eliminating potential contamination during transfer of materials into and from bioreactors or other sample environments.
...

Mouse Null for Pigment Epithelium-derived Factor (PEDF) (21077)

Investigators at Northwestern University created an animal model useful for analyzing the role of pigment epithelium derived factor (PEDF), an endogenous inhibitor of angiogenesis, in the onset and progression of diseases of the prostate, eye and brain. This heterozygous PEDF mouse represents the only currently available biological model for benign prostatic hyperplasia. Furthermore, the PEDF null mice show abnormalities in multiple tissues including the prostate, retina, kidney, and cerebellum.
...

Bioreactor (Cartridge) for in vitro Pharmacokinetic/Pharmacodynamic System

Abstract (Set) JHU scientists have developed a special bioreactor (cartridge) that permits growth of protozoan parasites for studies on the pharmacokinetic/pharmacodynamic (PKJPD) relationships of existing drugs and of new agents in development. The unit may be modified by changing the nature of the semi-permeable barrier, by adding extra ports for modulating the environment within the cartridge and may be miniaturized in size to reduce reagent requirement and permit the entire system to be run
...

Screening Method for Smith-Lemli-Opitz Syndrome

Using this method it is possible to determine, from a small blood sample taken from a newborn, whether there are abnormalities in the sterol content of the blood. The overabundance of certain sterols and the lack of others is a reliable test for Smith-Lemli-Opitz syndrome, a hereditary condition which leads to mental retardation and which can be ameliorated if detected early. Additional information available upon request.
...

Precision Genetic Regulatory Elements

New versions of the LacI repressor protein with increased DNA binding affinity. The lac repressor protein (LacI) is a genetic regulatory protein widely used to control the expression of cloned genes and is the prototype for negative control of transcription initiation in E. coli. The lac system has been used for many commercial purposes that benefit from the inducible control of recombinant protein expression. In the production of pharmaceuticals, LacI is used to regulate the expression of desi
...

Cloning and Expression of Ginkgo biloba Levopimaradiene Synthase

Ginkgo biloba leaf extracts have been used for centuries to treat cerebrovascular and cardiovascular diseases. Recently, the American Medical Association endorsed the Chinese herb as a viable alternative to traditional approaches in the treatment of Alzheimer�s Disease. Recent studies report that the extract delayed the progression of dementia in approximately one third of the patients studied. The beneficial pharmacological effects imparted by the extract are believed to be due, in part, to t
...

Diterpene Production in Recombinant Yeast

Through genetic modification of Saccharomyces cerevisiae and defined culture conditions, the production of diterpenes through fermentation has been achieved. This provides an opportunity to synthesize rare or novel diterpenes in a cost-effective manner. Additional information available upon request
...

TGF-Beta Conjugated Growth Scaffolds

TGF-Beta Conjugated Growth Scaffolds can be used to increase the mechanical strength of the cultured tissues by increasing extracellular matrix production. As the growth factor remains on the scaffold, at the site of tissue growth, the resulting tissues demonstrate increased mechanical integrity as compared to tissues grown with unlinked TGF-Beta, without sacrificing cell adhesion. In addition to tissue engineering and regeneration, this technology is also useful for wound healing applications u
...

Prefabricated Tissue Engineering Bone Plug for Bone Defect Repair

Composed of materials for engineering bone tissue, this plug may be inserted into a bone defect and then used to inject and hold in place polymeric materials that enhance bone regeneration. Other applications for this base technology include dental implants, vertebral fusion, and bone anchor implants for attachment of prosthetics.
...

Encapsulation of Radioisotopes in Carbon Nanocages for BioMedical Applications

Abstract Encapsulation of Radioisotopes in Carbon Nanocages for BioMedical Applications Technology ID: #23017 Investigator: Prof. Boris Yakobson Delivery of radioactive isotopes to specific targets in the body is important in the diagnosis and treatment of a wide array of diseases. Major problems with radiopharmaceuticals, however, are the chemical reactivity of these compounds, and the relatively high level of toxicity they possess. One approach to dealing with these problems is to encap
...

Non-Invasive Bone and Joint Damage Detection Device

Radiography is an important diagnostic tool in medicine but unfortunately suffers from certain limitations. X-ray detection equipment is not totally portable, cannot detect certain types of injuries and fractures, and requires patients be monitored for and protected from exposure to ionizing radiation. Also, imaging modalities like computed tomography (CT) for imaging hard tissue and magnetic resonance imaging (MRI) for analyzing soft tissue in order to detect damage, have their complications. B
...

Development of a Novel Confocal Laser Scanning Microscope for Functional Recording of Fast Neuronal Activity

Dendrites of neurons in the central nervous system are the principal sites for excitatory synaptic input. These neuronal dendrites are known to possess active computational properties also. Currently, these properties are mainly studied using well-known �patch clamp� techniques. However, the small number of sites that can be simultaneously accessed limits this approach. The inventors have developed an imaging system that allows for high-speed, multi-site optical recording from single neurons
...

Alternating Recombination Method for Assembling DNA Fragments

A process for recombination assembly of a series of cloned DNA fragments into a large ordered arrangement. Potential uses of this technology include: . Analysis of function of large interrupted coding regions . Construction of gene sets involved in complex metabolic processes . Chromosome engineering . Extensive genetic reprogramming of microbes for optimal production processes (metabolic engineering) . Large scale "editing" of known genomes based on engineering optimization considerations
...

Abstract Method for Assembling PCR Fragments of DNA

A process for assembling a series of DNA fragments generated by PCR into an ordered circular arrangement for replication and genetic work in cells. The method uses the 3' overhangs created by excision repair enzymes to direct the sequential ligation of PCR fragments on a solid scaffold. The resulting assembled DNA is removed with a site-specific recombinase. Potential uses for this technology include: . Assembly of large DNA fragments . Automated assembly of DNA fragments . Construct deve
...

Nitric Oxide-Producing Hydrogel Materials for Preventing Arterial Blockage

Endothelial cells, normally present as a monolayer in the arterial wall, are believed to play an important role in the regulation of smooth muscle cell proliferation in vivo. Endothelial cells are seriously disrupted by most forms of vascular injury, including by angioplasty and similar procedures. Approximately 35 to 50% of patients treated by percutaneous transluminal coronary angioplasty experience significant re-narrowing of the artery, termed restenosis, within six months of the initial tre
...

Metabolically-Enhanced Microbial Strain

The key element in this new technology is that metabolic energy which would normally be diverted away from biosynthetic processes is recaptured in the form of NADH. During fermentation with this strain, significant increases in many metabolites have been observed. Also, the strain behaves as if it is under anaerobic conditions even when oxygen is present, opening the way to producing anaerobic fermentation products under aerobic conditions.
...

Bacteriophage Genes as the Basis for New Antibiotics

Discovery of wide-spectrum bacteriocidal proteins creates new opportunities for (a) improving phage therapy and (b) identifying important bacterial drug targets and developing new antibiotics. These bacteriocidal proteins are of interest due to the fact that the bacterial cells do not lyse (burst) upon dying, a factor which makes their use against strains that harbor endotoxins much safer for the infected patient.
...

MUC7 20-mer Peptide: Novel and Promising Antifungal and Antibacterial Therapeutic Agent

This invention encompasses peptide derivatives of a naturally occurring protein found within the human body. These peptides have been shown to posses potent antifungal and antibacterial activity comparable to that of currently used antimicrobial age nts. The microbes used within those studies include but are not limited to both resistant and non-resistant strains of C. albicans, C. glabrata, C. neoformans, S. mutans, E. coli, and P. aeruginosa. Preliminary data demonstrates that these peptides p
...

Recombinant Baculovirus that Produces Human Topoisomerase I

Introduction Eukaryotic topoisomerase I (Topo I) is capable of relaxing both negatively and positively supercoiled DNA. The enzyme catalyzes changes in the superhelical state of duplex DNA by transiently breaking a single strand. Technology description Dr James Champoux’s laboratory has generated recombinant baculovirus that express wild type and active-site mutant (Y723F) versions of the full-length human Topo I and a 70-kDa protein (Topo70) which is missing the first 174 NH2-terminal amino a
...

Cell line expressing functional alpha1D-adrenergic receptors

Introduction The alpha1D-adrenergic receptor (AR) is a major regulator of cardiovascular and central nervous system function. Targeting AR is thus of major interest to the pharmaceutical industry for developing new medicines to treat hypertension and neurological disease. However, it has been impossible to screen for potential new α1D–AR ligands because of the inability to obtain sufficient functional expression in cells. Technology description To overcome the lack of stable in vitro express
...

Continuous Production of Microcellular Fibers

Introduction In microcellular plastic foams, inert gas is dissolved in thermoplastic under high pressure. The forced evacuation of this gas during processing creates pores (or cells) within the final plastic. The pores are small enough that clarity of the plastic is unaffected, and because of the pores, much less starting material is required in microcellular plastics than in conventional plastics, greatly reducing weight and cost. As a wide range of pore densities is achievable, this technology
...

Microcellular Foaming of Cyclic Olefin Copolymer (COC)

Introduction Cyclic olefin copolymer (COC) is a commercially available thermoplastic. It possesses exceptional clarity in its natural form, similar to that of glass, and it has high moisture resistance and low absorption rates. This thermoplastic’s many qualities make it an exceptional candidate for many applications, ranging from optics to packaging. The ability to create microcellular plastic foams using COC would further increase its range of applications with a significant reduction in mat
...

Keratinocytes Derived from Human Embryonic Stem Cells

Summary The investigator has discovered a method for isolating and propogating keratinocytes from human embryonic stem (ES) cells. Production of an isolated keratinocyte culture proceeded step-wise from ES cell implantation in scid mice, followed by colony fragmentation of the embryoid body, circumferential migration and detection of temporal biomarkers in-vitro. During keratinocyte differentiation, biomarkers that could be detected included transcription factor p63 followed by biomarkers of a m
...

A Novel Class of HIV Therapeutics: Monoclonal Antibodies that Neutralize Previously Resistant HIV Primary Isolates

Summary Background: Molecular studies of the genomic organization of HIV-1 show that it encodes a number of genes common to all retroviruses, such as the gag, pol, and env genes. The env gene of HIV-1 produces a cleaved envelope glycoprotein (gp120) that is expressed on the exterior viral envelope surface. The binding and fusion of HIV-1 viruses with cells is mediated by specific interactions between the external subunit of gp120 and the CD4 receptor on the target cell surface. Studies have show
...

Small Molecule Transcriptional Activators: Lead Agents for Gene Therapy

Summary One difficulty that has been encountered with the use of transcriptional activation systems in gene therapy is that overexpression of transcriptional activators in cells typically inhibits gene expression-a process known as squelching. Researchers at Harvard University have overcome this problem by developing novel transcriptional activators that, when bound to a specific DNA binding domain, stimulate the transcription of specific in vivo target genes without attenuating the expression o
...

PTP-NP, a New Member of the Receptor Protein Tyrosine Phosphatase Family, Implicated in Development of Nervous System and Pancreatic Endocrine Cells

Summary The Flanagan Lab discovered a new member of the protein tyrosine phosphatase (PTP) family, called PTP-NP (for neural and pancreatic). The regulation of protein tyrosine phosphorylation is an important mechanism for developmental control. The cDNA sequence indicates a receptor-type transmembrane molecule with a single tyrosine phosphatase domain. PTP-NP was initially identified in a screen for potential cell-cell signaling molecules involved in early development of the nervous system. In
...

A New p53 Cell-Based Assay

Summary Harvard researchers developed a reliable, high-throughput screening assay to identify activators and inhibitors of p53. This cell-based luminescence assay measures the level of a reporter gene product under the control of a promoter containing a p53-binding site. P53 is a transcription factor that can activate multiple genes involved in cell cycle regulation, DNA repair and programmed cell-death. It is often described as the "gatekeeper" of cellular integrity and plays a central role
...

Modular Conditional Knockout: Precise Temporal Control of Protein Degradation in Living Cells

Summary Polyubiquitinated proteins in eukaryotes are directed to the proteasome for degradation. It is generally accepted that proteasome-mediated proteolysis depends strictly upon ubiquitination, as disruption of that process has been observed to halt proteolytic activity of the proteasome. Researchers at Harvard Medical School have made the striking observation that a non-ubiquitinated protein, if linked to the proteasome complex, will undergo efficient degradation. The invention provides meth
...

Novel Gadolinium nanoparticles for Magnetic Resonance Lymphatic Imaging

Introduction Gadolinium (Gd3+) is chelated with diethylenentriaminepentaacetyl (DTPA) to provide contrast in magnetic resonance (MR) imaging to identify pathogenic tissues. Unfortunately, the Gd3+-DTPA complexes approved for clinical use are cleared within a few minutes and do not provide sufficient concentrations or time in lymphoid tissues. Many pathogenic conditions, including the spread of cancer, require high-resolution lymphoid tissue images. Technology description Rodney Ho’s laboratory
...

Nanocellular Foaming of Ultra High Molecular Weight Polyethylene using Ultrasound

Introduction Ultra high molecular weight polyethylene (UHMWPE) is a thermoplastic consisting of molecules of incredibly long chains with molecular weights of several million. It has the highest impact strength of any thermoplastic, has a high resistance to corrosion, moisture, and abrasion, and has a friction coefficient similar to that of Teflon. The ability to create nano-structured UHMWPE materials could yield many useful and unique properties, including mechanical and optical properties, and
...

Production of Palladium Nanostructures with Shape Selection by Adding Bromide

Introduction Single-crystal, one-dimensional nanostructures of palladium are attractive as interconnects for fabricating nanoscale electronic devices. Polycrystalline, mesoscopic wires made of palladium have been utilized for resistance-based detection of hydrogen gas. However, polycrystalline wires containing gaps between adjacent grains often shrink after initial exposure to hydrogen and may cause random, irreversible changes to the resistance of a sensing device. It should be possible to over
...

Copper Chloride-mediated Synthesis of Silver Nanowires

Introduction Owing to the high electrical and thermal conductivity of bulk silver, the synthesis of Ag nanowires has been and continues to be an area of active research. The properties and applications of the nanostructures are determined by the shape, size, structure, and composition of the nanostructures. The presence of various ions has been shown to influence the shape and size of metallic nanostructures produced via the polyol method. Technology description Younan Xia’s laboratory has inv
...

A Microfluidic Device and Methodology for Rapid, Quantitative Determination of Analyte Concentration in a Fluid Sample

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications, especially for biosensors and environmental monitoring. One recent area of interest is point-of-care diagnostic devices. These devices provide fast, accurate, and inexpensive sample analysis. Recent advances have enabled a universal platform that is adaptable to a potentially limitless array of analytes and could lead to affordable health care devices, including for the developing worl
...

Platelet Clotting Force Array

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications, such as point-of-care diagnostic devices. These microscale diagnostic platforms will enable faster, cheaper, and more accurate patient evaluation for a wide array of parameters. One such parameter is the coagulation capacity of blood, for abnormalities in which can be indicative of a wide variety of pathologies. The ability to more quickly and easily assess platelet function would grea
...

Programmable Generation and Transport of Droplets on Microscale Surface Texture Ratchets

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications. Currently, many laboratory processes can be combined on a single chip commonly only millimeters in size while manipulating liquid volumes of picoliter size or less. In order to effectively perform the many processes that may be required of a microfluidic system, various tools are required, such as pumps, valves, mixers, and ratchets. In particular, the development of ratchets and other
...

Cell and Microparticle Separator Employing Patterned Grooves

Introduction Microscale enrichment and separation are important for various research, biomedical, and industrial uses. Conventional means for these processes typically require specific labels— either inherent to the structure or appended to it. Unfortunately, not every important substrate has a convenient label of its own, such as stem cells, and it costs additional time and money to develop and append appropriate and effective labels, such as antibody tags and magnetic beads. Sometimes, all
...

Signal Amplification Method for SPR-based Chemical Detection

Introduction Surface plasma resonance (SPR) is a general spectroscopic method for sensing refractive index changes near the surface of a metal film. Its sensitivity to these changes provides a versatile platform for the observation and quantitation of chemical reactions and intermolecular binding at the metal/solution interface. The generality of the technique has led to its application to a variety of chemical systems, including biological interactions and reactions. Technology description Rese
...

3-D Tissue Culture Chamber

Introduction Many of the seminal findings in cell and molecular biology have come from cultures of cells grown in two dimensions but the limitations of biology in just 2D are becoming clear. Cells in tissue are not just autonomous masses but consist of many interdependent cell types and their surrounding extracellular materials in which the complex and dynamic interplay between them determine of affect the tissues’ function, development and physiological balance. Researchers at the University
...

Active Biomimetic Cilia Using Nanowires

Introduction Critical challenges in emerging bio-fluidic devices lie in bio-compatible transport of small sample volumes and bio-reaction enhancement without damaging biomolecules. In nature, biological cilia are hair-like structures whose rhymic beating provides motility for cells and microorganisms. Mimicking the action of biological cilia for gentle fluid mixing could solve the problem of small volume mixing. Technology description University of Washington researchers have developed a system
...

Purified Compositions of Stem Cell Derived Differentiating Cells

Introduction Stem cells have a capacity for both self-renewal and the generation of differentiated cell types. Embryonic Stem (ES) cells are derived from cultures of inner cell mass (ICM) cells, and have the property of participating as totipotent cells when placed into host blastocysts. The development pathways that endogenous ICM cells or transferred ES cells take to tissue formation and organogenesis has led many to hope that these pathways can be controlled for the development of tissue and
...

HLA Homozygous Cells

Introduction Although stem cells have great potential in regenerative medicine, they can only be used effectively in recipients that have the same histocompatibility type or the cells will be rejected and eliminated by the host immune system. The histocompatibility type of an individual is determined by many genes located in the human leukocyte antigen (HLA) locus on chromosome 6. There are several different versions of the genes in the HLA locus, and since each individual has two copies of eac
...

Microfabricated Thermocouples for Microfluidics and Microreactors

Introduction Continued strong growth is projected within the biochip products and services marketplace worldwide. As the number and complexity of reactions and separations that are carried out using biochip products goes up, the need for precise temperature monitoring and control within the microchannels increases. The ability to directly incorporate sensitive and fast temperature measurement into a biochip device may lead to better separations and reaction control. Technology description The in
...

Profiled Regulatory Sites Useful for Gene Control

Introduction The invention provides methods for quantitative profiling of chromatin sensitivity to a DNA modifying agent. The invention also relates to methods for identifying regulatory sites in a genomic locus and to methods for determining chromatin architecture in a genomic locus. The invention also relates to the use of profiled regulatory sites, databases comprising the same, and their use in regulating gene expression, disease diagnosis and therapy, and identification of therapeutic drugs
...

Methods and Compositions for Diagnosing Hepatocellular Carcinoma

Introduction Currently, it is difficult to diagnose HCC. Methods employed generally rely on imaging techniques such as MRI, CT, and ultrasound and are of little use in detecting the disease in its earliest stages. As with most cancers, early detection of HCC would leave physicians with more treatment options and patients with a better prognosis. Technology description This invention detects HCC by assaying patient samples such as tissue, plasma, serum, etc. for the presence and level of specific
...

CNS Tissue-selective Transgenic Animal Mouse Model

Introduction A central nervous system (CNS) specific knockout system has been unavailable thus far due to technical limitations. Recent advances have provided exciting data based on the use a liver-specific promoter to provide deletion of specific genes only in that tissue. This has successfully translated the concept, "tissue-targeted gene function deletion" into practice and could be considered as proof-of-principle data for a tissue-specific knockout system. The next challenge will be develop
...

Scn1A knockout mouse-a genetic animal model for human epilepsy

Introduction Epilepsy is one of the most common neurological disorders. Its pathophysiology is poorly understood and the available pharmacotherapies are effective only in a fraction of patients with frequent adverse side effects. Severe myoclonic epilepsy of infancy (SMEI), also known as the Dravet syndrome, is caused by mutations that inactivate one of the alleles of the SCN1A gene encoding the sodium channels Nav1.1. Children with SMEI have seizures during the first year of life that ultimatel
...

BCRP expressing cell lines

Introduction The breast cancer resistance protein (BCRP) is an ATP-binding cassette efflux transporter originally identified by its ability to confer multidrug resistance in cancer cells. BCRP mediates the efflux transport of a broad spectrum of substrates, including many drugs routinely administered to pregnant women to treat various diseases. Given its tissue localization pattern, BCRP is expected to play an important role in absorption, distribution, and elimination of drugs. Indeed, the impo
...

Dact1 (Dpr1/Frodo1) Conditional Knockout Mice

Introduction Wnts are secreted signaling proteins important in development and disease in multicellular animals. These signaling proteins help regulate cell fate specification, cell morphology, cell proliferation, cell migration, cell polarity, tissue patterning, and therapeutic processes such as stem cell biology and wound healing. Misregulation of Wnt signaling in people is linked to a wide variety of diseases including polycystic kidney disease, ulcerative colitis, schizophrenia, many forms o
...

M1 Muscarinic Acetylcholine receptor Knockout Mice

Introduction Muscarinic acetylcholine receptors are members of the G protein-coupled receptor superfamily expressed in neurons, cardiomyocytes, smooth muscle, and a variety of epithelia. The M1 receptors are predominantly expressed in the CNS and are the most abundant muscarinic receptor subtype in the cerebral cortex. They are extensively involved in cortical cognition and learning-induced or experience-dependent cortical plasticity. Technology description Dr Neil Nathanson’s laboratory has g
...

A Mouse Model for Osteoporosis

Introduction Osteoporosis, an age-related disease in which loss of bone mass causes skeletal deformities and fractures, affects nearly 20 million people in the US, mostly women. A major complication is a fracture of the thigh bone, in which case 20-30% of patients die in the year following the fracture. Half of the survivors remain disabled to some degree. Development of new therapies for this disease requires the appropriate animal model, one of which is described here. Technology description S
...

Type III Adenylyl Cyclase Gene Knockout Mice

Introduction The olfactory sensory system has the remarkable capacity to detect and discriminate among a wide array of odorants and translate them into cognitive representations. Cyclic nucleotide–gated ion channels in olfactory sensory neurons (OSNs) are hypothesized to play a critical role in olfaction. Type III adenylyl cyclase (AC3) is obligatory for olfactory signaling and this enzyme is likely the primary source of cAMP required for olfactory signaling. Technology description Dr Daniel S
...

Zinc Transporter-3 Knockout Mouse

Introduction The presence of histochemically reactive zinc within synaptic vesicles defines a subset of neurons that have been termed zinc-enriched (ZEN) neurons. Because ZEN neurons are prevalent throughout the limbic system and zinc is capable at physiological concentrations of exerting neuromodulatory effects on receptors thought to be involved in learning and memory, synaptically released zinc has been considered to play an important role in memory formation. Zinc transporter, ZnT3 is a defi
...

PDL2-Ig Fusion Protein

Introduction T cell activation and tolerance are critically regulated by costimulatory molecules, especially those in the B7 and CD28 superfamilies. PD-1, a novel member of the CD28 family, is expressed on activated T cells and B cells. PD-1 has been shown to be a negative regulator of T cell activation and is crucial for maintaining immune tolerance. PD-1 deficiency in mouse results in spontaneous autoimmune diseases. Moreover, PD-1 deficiency or blockade accelerated autoimmune diabetes on NOD
...

B7-H-Ig Fusion Protein

Introduction T cell activation and differentiation require not only T cell receptor recognition of the antigen–MHC complex but also costimulation through the interaction of accessory molecules on APC and their corresponding receptors on T lymphocytes. CD28 is the most important costimulatory receptor on T cells. It binds to B7.1 (CD80) and B7.2 (CD86) on activated APC, that are highly regulated after innate activation. Technology description B7-H-Ig fusion protein was generated in Dr Chen Dong
...

Type I Adenylyl Cyclase Knockout Mouse

Introduction The type I adenylyl cyclase (I-AC) is neurospecific and expressed in areas of the brain that show long-term potentiation. This enzyme is thought to play an important role in the development of the central nervous system, circadian rhythm, and memory formation. Technology description Dr. Daniel R. Storm’s laboratory has generated I-AC knockout mice. A gene-targeting vector encoding the disrupted N-terminal of I-AC was constructed and used for homologous recombination in AB1 embryon
...

A Yeast Ligand Sensor

Introduction The yeast ligand sensor is a novel approach for the detection and quantification of ligandbinding, an issue of great interest in drug discovery and other areas. The approach is a protein-derived biosensor in which the readout is a change in growth of temperaturesensitive yeast. The yeast lack dihydrofolate reductase (DHFR) and are complemented by mouse DHFR containing a ligand-binding domain inserted in a flexible loop. The yeast grow poorly and are temperature-sensitive, due in lar
...

NPY-Deficient mice

Introduction NEUROPEPTIDE Y (NPY), a 36-amino-acid transmitter distributed throughout the nervous system, is thought to function as a central stimulator of feeding behaviour. NPY has also been implicated in the modulation of mood, cerebrocortical excitability, hypothalamic– pituitary signalling, cardiovascular physiology and sympathetic function. Technology description Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical
...

SirT1 Conditional Knockout Mice

Introduction SirT1 is a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase capable of modifying the activity of many transcription factors that have been implicated in the physiology and pathology of multiple organs. Researchers at the University of Washington have developed a strain of SirT1 conditional knockout mice (SirT1co/co mice). By crossing SirT1co/co mice with CMV-Cre transgenic mice, they generated SirT1-deficient mice and uncovered the function of SirT1 in modulation of est
...

Thrombospondin 2-null mice

Introduction Thrombospondin 2 (TSP2), a matricellular protein with a primary role in modulating cell matrix interactions, has been implicated in tissue repair and foreign body responses. Technology description TSP2-null mice were produced by targeted disruption of the thrombospondin 2 gene in embryonic stem cells by homologous recombination. Correctly targeted ES cells were microinjected into C57BL/6 mouse blastocysts and the resulting male chimeras were mated with C57BL/6 females. Agouti progen
...

SV2B Knockout Mouse

Introduction Synaptic vesicle protein 2 (SV2) is a membrane glycoprotein found only in the secretory vesicles of neural and endocrine cells. It appears to be unique to vertebrates, suggesting that it evolved as a part of complex signaling systems. In mammals, there are three SV2 genes denoted A, B, and C. The molecular mechanism by which these proteins regulate secretion is not clear. Based on predicted structure and amino acid sequences, the SV2 proteins belong to the major facilitator superfam
...

Cyclic nucleotide phosphodiesterase 7B (PDE7B)

Introduction Cyclic nucleotide phosphodiesterases (PDEs) are enzymes hydrolyzing the second messengers adenosine and guanosine 3',5'-cyclic monophosphates (cAMP and cGMP). The human genome encodes 21 PDE genes that are categorized into 11 families. Selective inhibitors against individual PDE families have been developed as therapeutics for treatment of various human diseases. Technology description This technology provides the mouse cDNA clone for PDE7B, a cAMP specific PDE. In mice, PDE7B trans
...

SV2A/B Double Knockout Mouse

Introduction Synaptic vesicle protein 2 (SV2) is a membrane glycoprotein found only in the secretory vesicles of neural and endocrine cells. It appears to be unique to vertebrates, suggesting that it evolved as a part of complex signaling systems. In mammals, there are three SV2 genes denoted A, B, and C. The molecular mechanism by which these proteins regulate secretion is not clear. It has been proposed that the SV2s perform a redundant function, an argument supported by the apparent co-locali
...

Fluorescent Microglial Cell Lines

Introduction Microglial cells are the resident immune cells of the central nervous system. Upon brain damage, these cells are rapidly activated and transform from a resting state into activated microglia. They share many properties with peripheral tissue macrophages, one of them being their inherent difficulty to be transfected or transduced. Currently there is no stable transgenic microglia cell line at all. Researchers at the University of Washington have developed a viral-based transduction s
...

A Microfluidic Device and Methodology for Rapid, Quantitative Determination of Analyte Concentration in a Fluid Sample

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications, especially for biosensors and environmental monitoring. One recent area of interest is point-of-care diagnostic devices. These devices provide fast, accurate, and inexpensive sample analysis. Recent advances have enabled a universal platform that is adaptable to a potentially limitless array of analytes and could lead to affordable health care devices, including for the developing worl
...

Platelet Clotting Force Array

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications, such as point-of-care diagnostic devices. These microscale diagnostic platforms will enable faster, cheaper, and more accurate patient evaluation for a wide array of parameters. One such parameter is the coagulation capacity of blood, for abnormalities in which can be indicative of a wide variety of pathologies. The ability to more quickly and easily assess platelet function would grea
...

Tissue-Engineered Urinary Bladder Using Autologous Cells

Introduction There is a variety of reasons that a person’s bladder may fail or require removal, including bladder acontractility, cancer, and cystitis. Unfortunately, conventional artificial bladders tend to suffer from critical complications, such as inadequate performance, infection, and adverse host response. Ideally, a new bladder could simply be formed from the patient’s own cells. Technology Description Professor Ratner at the University of Washington has developed a technique to creat
...

Advanced Biomimetic Surfaces for Surface-Enhanced Raman Spectroscopy (SERS)

Introduction Raman spectroscopy characterizes molecules by their Raman scattering. As incoming light scatters off a molecule, most does so elastically, neither gaining nor losing energy, and this is known as Rayleigh scattering. Some of this light, however, about 1 in 10 million photons, is the subject of an energy exchange with the molecule and scatters inelastically, possessing more or less energy than it started with, and this is known as Raman scattering. Raman scattering acts as a vibration
...

Respiratory Detection System (RDS)

Introduction Respiration rates are an important indicator of cellular and metabolic states in cells ranging from mammalian to bacterial, from individuals to large populations. Unfortunately, conventional techniques have significant drawbacks: for instance, electrochemical techniques have low sensitivity and consume oxygen and optical techniques are difficult to integrate. With more effective monitoring techniques, the detection of microorganisms, the characterization of disease states, and the c
...

New biocompatible and biodegradable polymers

Introduction Porous biodegradable scaffolds are ideal materials on which, new tissue may be grown. These new tissues are used as graft material to replace damaged tissue removed during surgery. These polymers may also be used in drug delivery systems and for medical device coating. Technology description Researchers at the University of Washington have developed a new family of biodegradable, biocompatible polymers made of repeating subunits of amino acid monomer units and low molecular weight p
...

Fluid flow assisted dielectrophoretic alignment and sorting of dielectrophoretic alignment and sorting of

Introduction The accurate and precise sorting and deposition of nano/bio materials within a nano- or micro-environment is critical for the functionality of devices such as biochips. Fluid flow in a microchannel is one method of controlling long-range transport and electric fields may be employed to control short-range positioning of nano/bio materials. Technology description Researchers at the University of Washington have developed an alignment and sorting method of nano/bio materials using flu
...

Clutter Rejection Filters for Doppler Optical Coherence Tomography

Introduction Doppler optical coherence tomography (DOCT) is a recently developed technology that can image blood flow with high special and temporal resolution. Technology description Researchers at the UW have developed techniques to improve the sensitivity of DOCT for real-time flow imaging by using cutter rejection filters. The filters reduce the signal scattering effect of moving blood cells and vessel walls. Business opportunity Results have shown that the accuracy in determining the small
...

Flexible Fiber-Optic Scanning Multiphoton or Harmonic Miniature Imaging Probe

Introduction Endoscopes are increasingly involved in performing common surgical procedures and in diagnosing cancer and other medical conditions. New technologies can demonstrate significant cost savings by moving these procedures from an inpatient to an outpatient setting. Technology description Researchers at the UW have developed a new miniature, flexible, fiber-optic scanning probe for real-time multiphoton fluorescence or other non-linear (such as second or higher harmonic generation) imagi
...

Dynamic Change of Focus for True 3-D, Scanned Retinal Displays

Introduction Generating true 3-D computer displays will enable the creation of more realistic and informative images, allowing improved functionality when relying on visual display data. Technology description Researchers at the UW have developed the use of optical elements to dynamically adjust the wavefront of scanning beams of light for scanned retinal displays. These elements adjust the phase of the wavefront spatially across the beam, changing the focal point of the beam. The virtual retina
...

Design of Surface Plasmon Biosensor Based on Self-Referencing and Digital Window System

Introduction Surface Plasmon resonance (SPR) has attracted strong attention as a biosensor in the past two decades because it can provide label-free, real-time detection with high sensitivity. Technology description Researchers at the UW have developed an SPR biosensor with increased accurate referencing. In this design sensing and referencing materials are prepared adjacently in one channel so the bulk affect for the sensing and referencing surface are almost the same. As a result, a more preci
...

Intracellular Delivery Vector

Introduction Some drugs require cellular uptake to be effective and methods to increase cellular uptake are desired to improve on the efficacy and reduce the side-effects of certain drugs. One way to increase cellular uptake of a drug is by attaching it to a cellpenetrating peptide (CPP). CPPs, such as the TAT peptide from HIV, have been used extensively in the study of cellular uptake. Constructing fusions between CPPs and the target drug can be difficult and time consuming; additionally, it ap
...

Thin-film Bioreactor

Introduction The large scale production of biological materials requires the use of bioreactors. Bioreactors can also be used in biologically mediated chemical conversions and waste management. Traditionally, bioreactors are difficult to fully automate and require labor intensive maintenance to function normally over long periods of time. Technology description A novel design bioreactor and process for automating protein production has been invented by researchers at the University of Washington
...

Protein Microarray Fabrication with Novel Functionalization

Introduction Protein microarrays are enjoying increasing use in both research and diagnostic applications. One of the key challenges in protein microarray manufacture is the fixation of the protein or the protein ligands of interest on the microarray surface with a robust linkage that doesn’t alter the protein properties. Technology description The inventors have developed a method to functionalize metallic gold surfaces that allow proteins to be covalently coupled to the surface in pre-select
...

Targeted Gene Modification by Parvoviral Vectors

Introduction Though the development of integrating vectors based on eukaryotic viruses has made possible the efficient introduction of genes into mammalian chromosomes, there are many situations where it would be preferable to modify specific chromosomal sequences. This ability could eliminate unwanted chromosomal genotypes and avoid position effects on gene expression. The need for such an ability to modify a preexisting locus is particularly acute in gene therapy, where mutant genes can have d
...

A method for quantitative, proteome-wide analysis of protein phosphorylation

Introduction Protein phosphorylation is one of the most important regulatory events in cells. The state of activity of numerous enzymes and processes and the association of specific proteins into functional complexes are frequently controlled by reversible protein phosphorylation. Because cellular proteins are coordinately phosphorylated to control specific biological processes, the complex mechanisms that control biological systems by protein phosphorylation are difficult to investigate using c
...

Carbon Nanotubes by Electrospinning with a Polyelectrolyte and Vapor Deposition Polymerization

Introduction Ever since the discovery of carbon nanotubes they attracted a great deal of attention due to the myriad of possible applications, such as in fuel cells, lithium-ion batteries, and supercapacitors. Nevertheless, the synthesis of well-defined carbon nanotubes of 50 to 100-nm diameter has been a long standing goal of material chemistry. Technology description To reach this goal, UW researchers have developed a method that allows fabrication of carbon nanotubes with well-controlled diam
...

Carbon Nanotubes by Electrospinning with a Polyelectrolyte and Vapor Deposition Polymerization

Introduction Ever since the discovery of carbon nanotubes they attracted a great deal of attention due to the myriad of possible applications, such as in fuel cells, lithium-ion batteries, and supercapacitors. Nevertheless, the synthesis of well-defined carbon nanotubes of 50 to 100-nm diameter has been a long standing goal of material chemistry. Technology description To reach this goal, UW researchers have developed a method that allows fabrication of carbon nanotubes with well-controlled diam
...

Solution Processable n-Type polymers

Introduction Conjugated polymers and organic semiconductors promise great advances in electronics because of the benefits they offer over silicon, including being cheaper with less demanding manufacturing requirements and allowing for larger, more flexible, and more robust materials that are more responsive to chemical and biochemical stimulus. A critical roadblock in developing this technology, however, is the lack of easily synthesized n-type polymers and, subsequently, the lack of effective p
...

Microporous Micromolding

Introduction In microcellular plastic foams, inert gas is dissolved in thermoplastic under high pressure. The forced evacuation of this gas during processing creates pores (or cells) within the final plastic. These cells are small enough that clarity of the plastic is unaffected, and because of the cells, much less starting material is required for creating low-density microcellular plastics than for creating conventional plastics, greatly reducing weight and cost. As there is a wide range of po
...

3-D Tissue Culture Chamber

Introduction Many of the seminal findings in cell and molecular biology have come from cultures of cells grown in two dimensions but the limitations of biology in just 2D are becoming clear. Cells in tissue are not just autonomous masses but consist of many interdependent cell types and their surrounding extracellular materials in which the complex and dynamic interplay between them determine of affect the tissues’ function, development and physiological balance. Researchers at the University
...

A Novel Catalyst Technology for the Polymerization of ?-Olefins

The Invention A novel catalyst technology for the polymerization of alpha -olefins was developed including several families of pre-catalysts based on early transition metal complexes of amine-phenolate ligands. These pre-catalysts, when activated by various co-catalysts under mild reaction conditions, exhibit a broad range of reactivities in polymerization of a variety of alpha -olefin monomers. These include: Highly active catalysts for atactic polimerization of long chain alpha -olefin mo
...

Increased Fungal Biomass for Bio Ethanol Production

The Invention Dr. Amir Sharon and his team from Tel Aviv University have developed a transformation-based approach through which fungi can be modified to be less sensitive to external conditions and environmental stresses, have increased sustainability in culture during fermentation, and have both enhanced growth rate and spore production. As a result, the fungal cultures exhibit a dramatic increase in fresh and dry biomass production, reduced sensitivity to stress conditions, enhanced spore pr
...

A Potential Target for Discovery and Development of Novel Antiviral Agents Against Porcine Endogenous Retrovirus

The Invention Researchers in Tel Aviv University have cloned and characterized Reverse Transcriptase (RT) of PERV-B - a variant that infects human cells and, hence, might infect human recipients of porcine tissues. The recombinant PERV-RT is expressed in bacteria, giving large amounts of soluble and highly active enzyme, which is relatively insensitive to nucleoside analogues inhibitors (NRTIs) and non-nucleoside analogue inhibitors (NNRTIs). The availability of an active, purified recombinan
...

Self Assembled Peptide Hydrogel

The Invention A breakthrough Self Assembled Peptide Hydrogel (SAPH) has been developed by Professor Ehud Gazit and his team at Tel Aviv University. SAPH is a safe, self assembled, biocompatible, remarkably strong hydrogel with modulated stability that is suitable for various medicinal and cosmetic applications The Need SAPH is an outstanding rigid self assembled peptide hydrogel based on the highly efficient self-assembly of the short aromatic peptides diphenylalanine. The discovery and dev
...

Self Assembling Peptide Nanotubes

The Invention Aromatic dipeptides have been demonstrated to self-assemble into discrete nanotubes that are, upon design, either sensitive or resistant to enzymatic degradation. Such nanotubes are homogeneous, with micron-scale persistence length and can serve as molds for conductive nanowires suitable to various applications. Design of the building-blocks allows the assembly of peptide into closed-cage nanospheres. The Need There is an acute need for a reduction in scale in areas as diverse a
...

Edible Anti-Adhesion Material for the Treatment of Oral Bacterial

The Invention A high-molecular-weight, non dialyzable material (NDM) isolated from Cranberry juice, specifically inhibits adhesion and dissociates co-aggregates of oral bacteria. NDM act preferably on co-aggregates that include Gram-negative anaerobes and inhibit, in-vitro, biofilm formation by mutans streptococci. In a clinical study, salivary mutans streptococci count, as well as total bacterial count, was significantly reduced by NDM containing mouthwash as compared to control group using pl
...

Edible Anti-Adhesion Material Derived from Cranberry for the Treatment of Helicobacter Pylori Infection

The Invention An edible, high-molecular-weight, non dialyzable material (NDM) isolated from Cranberry juice inhibits specific adhesion of H. Pylori to immobilized human mucus and to cultured gastric epithelial cells. No relationship was observed between the resistance to antibiotics and the anti-adhesion effect of NDM. The anti-adhesion activity of NDM provides a novel approach for the treatment of gastric ulcers caused by H. Pylori. The Need Helicobacter Pylori (H. Pylori) is the major ca
...

Over-Expression of Membrane Proteins in Bacterial System

The Invention The invention provides for an increase in the expression of heterogeneous membrane proteins as well as crystallization ability. This is achieved by addition of hydrophilic alpha-helical segments to the desired membrane protein, via a cloning procedure. Two different helices enable construction of eight derivatives of the desired protein. The helices facilitate crystallization by providing the necessary hydrophilic surfaces for crystal formation. The Need Membranes are the site f
...

Targeted filamentous bacteriophages as therapeutic agents

The Invention Targeted drug-carrying filamentous bacteriophages have been developed as a novel therapeutic approach for the treatment of infectious diseases and cancer. The phages are genetically-modified to display an antibody or a targeting peptide as a targeting moiety on their surface and are used to deliver a cytotoxic drug to the target. The drug is linked to the phages by means of chemical conjugation through a labile linker subject to controlled release. In the conjugated state the cyto
...

Novel baby bottle nipple that mimics breast feeding

The Invention A new nipple for baby bottles has been developed that mimics breast feeding. The structure of the new nipple is based on the functional architecture of the breast nipple anatomy, thus enabling mimicking the physiological suckling pattern of milk from the mother's nipple. The breastfeeding-like feature of the new nipple allows milk suckling by the baby without complete sealing of the lips around the nipple in order to generate the suction pressure gradient. Potential Applications
...

Novel oral formulation of Insulin

The Invention Gagomers are novel particles (neither liposomes nor microspheres/nanospheres) composed of hyaluronan shell which is crossed linked to phospholipids and can be generated either as micro or nano particles. The research team at Tel Aviv University has demonstrated that: (1) Gagomers efficiently encapsulate fibrillar Insulin (2) gagomers are protecting the insulin in the GIT (3) Due to the hyaluronan shell Gagomers are targeting the Insulin to intestinal mucosa and show muco-adhesiv
...

Collagen fibers from a soft coral

A novel source of collagen manifested as fibers derived from certain species of soft corals has been isolated. The New Collagen fibers are strong and flexible compared to commercial collagen. The fibers have a high reversible extensibility compared to mammalian collagen fibers and can be reversibly stretched to a strain 2-3 fold greater than known collagen fibers. The fibers extend without collapsing up to 20% of their original length as opposed to other collagens that may reach up to only 8-1
...

Novel Antimicrobial Peptides

BACKGROUND Ai2 Limited is a University of Manchester spinout company that aims to become a significant player in the global anti-infectives market. The company’s focus is on developing novel anti-microbial products, for out-licensing to healthcare companies across a range of sectors. THE TECHNOLOGY The core technology stems from research into a class of human proteins involved in innate immunity, whose role in fighting infection had not previously been identified. Specific regions of these pro
...

Corticosteroids with Reduced Side Effects

BACKGROUND Glucocorticoids (GCs) have been used therapeutically for many years to treat a range of inflammatory conditions as immunosuppressants and in the treatment of certain cancers. These compounds are the most potent and effective anti-inflammatory agents known. Their use has been limited however by their multiple and often devastating side effects. As anti-inflammatory agents, GCs have two primary modes of action. One is responsible for the unwanted side effects and the other for the desir
...

Opticin a Treatment for Cancer and Angiogenesis

BACKGROUND Opticin is a protein present in the eye (in the vitreous humour) and was discovered at University of Manchester by Dr Paul Bishop. Uniquely Opticin has been found to exhibit both cytotoxic AND anti-angiogenic (blood vessel formation) effects on a range of cell lines from different cancers. Currently there is considerable interest in developing drugs derived from natural endogenous molecules with cytotoxic/anti-proliferative effects for treating cancer, as these are less likely to have
...

A Functional Food with Indications for Peptic Ulcers

BACKGROUND 1 in 10 people are affected by peptic ulcers over the course of their lifetime. Incidence of the condition is set to increase as the population ages. Current treatment relies on combination antibiotic therapy. However, a 20% failure rate and increasing antibiotic resistance highlight the need for a new therapeutic paradigm. THE TECHNOLOGY Prof Ian Roberts’ group at the University of Manchester are developing natural extracts for the treatment and prevention of peptic ulcers that spe
...

Novel Tissue Marker for Stratifying

BACKGROUND Research at the University of Manchester has identified a new tissue marker with potential use in the stratification of breast cancer patient groups and improved targeting of treatment for each patient. THE TECHNOLOGY The human homolog of the suppressor of deltex protein (SDRP) is aberrantly expressed in breast cancer cell lines. SDRP is thought to be a ubiquitin ligase and is a component of the Notch signalling pathway, which plays a key role in regulating cell proliferation. Express
...

Spinal Disc Nucleus Replacement

BACKGROUND In the last 10 years commercial interest in treatment of spinal degeneration in relation to back pain has increased with a large number of devices competing in a market estimated to be worth more than $3.5bn and growing at 15 - 20% pa. This technology being developed at the University of Manchester has a number of advantages over competitors. THE TECHNOLOGY Nucleus replacement or repair: this technology will replicate the function of the natural nucleus of the spinal disc whilst maint
...

Production of Novel Human Collagens

BACKGROUND A powerful new platform technology for the production of human collagens is being developed in the Kadler Centre for Cell-Matrix Research within the Department of Life Sciences at the University of Manchester. The collagens most closely mimic human collagen structure, giving a far superior product to that produced by the harsh conditions necessary to extract animal collagens and can be tailored to incorporate binding sites for growth factors and cell attachment to promote integration
...

eLUNSERS: a SOFTWARE TOOL FOR MONITORING THE SIDE-EFFECTS OF NEUROLEPTIC DRUGS

BACKGROUND Neuroleptics are the drugs of choice in the treatment of schizophrenia, bipolar mania and related disorders but suffer from troublesome side-effects such as drug induced parkinsonism, restlessness, facial tics etc. The severity and patient acceptability of these effects varies widely. Research by Dr Jennie Day into the side-effects of neuroleptic drugs has led to the development of a widely recognised questionnaire-based tool, the LUNSERS Scale, for monitoring patients on such medicat
...

Collaboration/Licence Opportunity Size and Permeability Selective Encapsulation and Delivery of

BACKGROUND Although there is considerable research being carried out in the area of hollow sub-micron constructs for the purposes of encapsulation of useful therapeutic entities, most of the processes developed to date for the preparation of such constructs are incompatible with maintaining the functionality of sensitive biomolecule payloads. In addition in many clinical conditions it would be useful to be able to deliver and maintain biological activity of the therapeutic agent proximal to the
...

Novel Anti-helminthic

BACKGROUND Gastrointestinal nematode infections are amongst the commonest infections of man and animals worldwide. Collectively 1 in 3 people on the planet are infected by at least one species and approximately 50% of these are school age children In the developing world they affect the well being of children and their susceptibility to other disease. Researchers at the University of Manchester have shown that treatment of animals infected with parasitic worms with a known pharmaceutical compoun
...

Exciplex™ a Highly Sensitive Nucleic Acid Fluorescent Detection System

BACKGROUND Conventional fluorescent dyes used in nucleic acid detection methods suffer from a number of limitations. High backgrounds result in false positives and a reduction in sensitivity. The small Stokes shift (difference between excitation and emission wavelengths) often requires optical filters to be used that further reduce sensitivity. Several detection techniques (Molecular Beacons, Sniper etc) also require the use of long oligonucleotide sequences whose design complexity can limit the
...

Enzyme Targetted Therapeutics

BACKGROUND Enzymes, especially proteases play essential roles in disease specific processes and are valuable targets for therapeutic intervention. A number of methods of targeting enzymes have been used to date. These include specific enzyme inhibitors and polymeric carriers which disintegrate to release pro-drug in response to disease related enzymes. Dr. Rein Ulijn of the School of Materials at the University of Manchester has developed bio-responsive hydrogels that selectively expand on conta
...

Kenaf plants with elevated fiber content

The Invention Transgenic Kenaf plants (Hibiscus cannabinus L.) have been developed by modifying the endogenous concentration of gibberellins using anti-sense technologies. These novel Kenaf plants have elevated fiber content. Furthermore, the fibers in these plants are of higher quality, substantially exceeding those of the methods used today. Potential Applications Paper Cordage (cords and rope) Textiles Advantages Kenaf is a fast growing annual closely related to cotton (Gossypiu
...

ZNF206: a Novel Regulator of Embryonic Stem Cell Self-Renewal and Pluripotency

ZNF206: A Novel Regulator of Embryonic Stem Cell Self-Renewal and Pluripotency
...

Life Extension Through Neurofibromin Mitochondrial Regulation and Antioxidant Therapy for Neurofibromatosis-1 in Drosophila...

Background: Anti-oxidant and anti-aging compounds have been screen using other animal models. However, there was no clear understanding of the molecular basis for how the screening worked and the systems used were not robust. Our system specifically targets the mitochondria in a well defined manner. Technology: Drosophila that are deficient in the Neurofibromatosis 1, a tumor suppressor gene, have a shortened life span. We have shown that this is due to reduced CAMP production which decrease
...

Mitochondrial DNA Variants Associated with Metabolic Syndrome

Background: Over the past 25 years, the Wallace's laboratory discovered that the variation in the maternally-inherited human mtDNA forms a single mtDNA tree in which the branches of the tree are region specific. The mtDNAs in each major branch of the tree are thus a group of related haplotypes, called a haplogroup. These regional branches contain functional mtDNA mutations that permitted ancestral humans to adapt to different environments, and it is Dr. Wallace's belief that these "adaptive" va
...

Energy-Saving Technological Method for Long-Term Storage of Bio-Degradable Tissue

Background: Currently the critical storage of bio-degradable tissue for long periods of time is done at -70" C. This temperature does provide nearly unlimited storage time. Storage at -70' C is substantially more expensive as compared with that at -40" C Technology: The crucial part of this proposal is based on the idea that while the initial cooling to the temperature where the flip-flop will be accomplished (to or below approx. -70" C) is obligatory, this low temperature is not obligatory
...

Photo-electric Device and Method for High Throughput Activation, Guidance and Poration of Targeted Cells with High Spatial Resolution

Background: In biomedical research, controlled modulation of physiological functions of various excitable cells such as skeletal, cardiac and neuronal cells is important. The information derived from activation of these excitable cells under different chemical environments can lead to the evaluation of therapeutic drug efficacy. Further, there is a need for controlled poration of exogenous materials/genes into living cells. Various electrical, chemical and optical methods are recently being pur
...

Mouse Model of Cardiac and Muscle Disease and Cancer Caused by a Mitochondrial DNA Point Mutation

Background: It is becoming increasingly clear that mitochondrial DNA (mtDNA) base substitution mutations are a common cause of age-related degenerative diseases as well as cancer. However, no mouse model has been generated in which a mtDNA base substitution has been stably introduced into the mouse germline and resulted in an inherited predisposition to and age-related disease or cancer. Technology: UCI researchers report the introduction of a mtDNA harboring a missense mutation in the COI g
...

Integrated Biomedical Multimodality Imaging Probe - Optical Coherence Tomography/Ultrasound/Photoacoustics Imaging System

Background: In clinical applications for the examination of cardiovascular, cerebrovascular, leg and other vessels, ultrasound imaging (USI) and optical coherence tomography (OCT) are two of the most widely used imaging modalities. USI and OCT both measure cross-sectional tissue profile. OCT measures tissue surface profile and cross-sectional image within a few millimeter depth range under the skin with a superior image resolution of 10 micrometers; high frequency USI also measures cross-sectio
...

Method and System of Elasto-Optical Tomography for Breast Cancer Imaging

Background: Currently, X-ray mammography is the widely accepted method for the detection of changes in the breast that may be cancer. However, this screening technique lacks specificity to determine whether detected abnormalities are either benign or malignant. A significant number of suspicious masses referred by mammography for surgical biopsy are in fact, found to be benign. These false-positive mammograms may cause patient anxiety and lead to increase healthcare costs. Technology: To ove
...

Accurate Classification of MLH1/MSH2 Missense Mutations with MAPP-MMR

Background: During genetic testing of the human MLHl and MSH2 genes, missense variants are sometimes encountered. It is difficult to tell if these variants are benign or deleterious. Technology: This project is a bioinformatic algorithm that interprets whether or not a genetic variant is a mutation or not for the Human genes MLHl and MSH2. Application: Screening for somatic mutations - cancer diagnostics
...

APE/REF-1 as a Drug Target for Acne and Development of Potential APE/REF-1 Inhibitors for Prevention and Therapy of Acne Vulgarsis

Background: Acne vulgarisis the most common skin disorder, and while it usually appears in adolescence, adults can get it too. The pathogenesis of acne is complex and multifactorial. According to the American Academy of Dermatology, the four basic mechanisms contributing to acne are hormones, increased sebum production, changes inside hair follicles, and bacteria. Acne occurs when hair follicles and the sebaceous glands inside the follicles are inflamed. Sebaceous glands make an oily substance
...

A Robust Reporter Ssystem to Identify Bone Morphogentic (BMP) Activity

Background: Bone Morphogentic Proteins (BMP)s are involved in various developmental processes, but are most famous for the activity to stimulate bone formation. Technology: Through phylogenetic footprinting and deletion analyses, UCI researchers identified a conserved BMP responsive element that specifically responds to BMP signaling, initially from the promoter of Xvent2, and subsequently from the promoter of Id3. These elements were multimerized and linked to a minimal promoter. These synt
...

Fiber Optic Probe and Method for Optical Trapping, Ablation, Excitation and Detection of Microscopic Objects

Background: Manipulation of microscopic objects by laser methods has been used since the 1970s. Both laser tweezers (used to hold microscopic objects in place) and optical scissors (used to conduct delicate surgery on the object in question) allow the scientists and engineers to perform minimally invasive manipulation of micro/nano objects which include single molecule, living cells and their organelles. Further, changing properties of light beam (e.g. circular polarization), the optical tweeze
...

Method and Apparatus for Performing Qualitative and Quantitative Analysis of Wounds Using Spatially Structured Illumination

Background: Use of instruments to assess etiology and status of chronic wounds is still in an embryonic state. Clinicians rely primarily on clinical features such as wound size, location, depth, and infection in order to make treatment decisions regarding the patient care. Several array of medical devices under investigation for wound care include Doppler ultrasound, Doppler perfusion imaging, transcutaneous measurement of tissue oxygen and near-infrared spectroscopy. One common drawback to the
...

Method to Develop Mutant Protein Library

Background: Current technologies used to develop novel protein and antibody libraries have been met with limited success. Specified and controlled distribution of mutations relative to the parent protein cannot be achieved through the use of oligonucleotides with degenerate base regions. Also DNA oligonucleotide synthesis using DNA base triplets requires specialized DNA synthesis machinery which is may not be efficiently utilized in miniaturized high-through-put screening. Technology: Univer
...

Novel Antenna Angiography Catheter

Background: Cardiac catheterization is typically performed with stiff-tipped catheters. In order to engage a blood vessel orifice, the stiff tip must be positioned at a precise location, and in the correct orientation. During the relatively extensive manipulation sometimes required for target vessel cannulation, the stiff catheter may cause vessel trauma to the wall of the blood vessel or the cardiac chamber. In addition, the opening of a target blood vessel may be difficult to locate with the
...

Fast Lysis Cell Traps

Background: Chemical cytometry involves the use of high-sensitivity analytical tools, such as mass spectrometry, electrochemistry and capillary or microfluidic separation methods to characterize single cells. In order to accomplish this analysis, it is desirable to selectively apply cells to predetermined regions of a substrate, also know as cell patterning. Chemical cytometry employing an electrophoretic separation has been used to measure a wide range of cellular properties; for example, quan
...

A Model of Heterogeneous Ventilation and Nitric Oxide Production in the Lungs

Background: Asthma is a pulmonary disorder characterized by chronic airway inflammation and airflow obstruction that is widespread, but variable in space and time. Although airflow obstruction is routinely monitored with spirometry, assessing inflammation in a non-invasive manner is more difficult. The concentration of nitric oxide (NO) is elevated in the exhaled breath of asthmatics, and has tremendous potential to be a non-invasive marker of inflammation. NO exchange in the lungs exhibits
...

Novel Method Using Laser in Assays to Induce Mechanotransduction

Background: Mechanotransduction, the process by which cells convert mechanical stresses to a biochemical signal, influences many cell functions including cell motility and proliferation and also modulates cell signaling events in the regulation of sensory neurons and in various molecular pathways such as the Src family of nonreceptor tyrosine kinases, ion channels, and the extracellular signal-regulated kinases. Mechanotransduction plays a role in the regulation of blood pressure and the progre
...

Ergonomic Tracheal Suction System

Background: Tracheal suction catheters used today by respiratory therapists have a number of drawbacks. First, the therapist operating the tracheal suction catheter is directly exposed to the patient's secretions and mucus due to the catheter's design. Second the design of the catheter causes the therapist to operate the valves with repetitive motions that may injure and/or damage the therapist's hand. Therefore a safer system of suction regulation and delivery is desirable. Technology: Univ
...

Nanosecond Laser Cell Lysis in Microfluidic Devices

Background: To date, cell lysis in microfluidic devices for analytical purposes has been achieved using chemical, electrical, and optical means. Chemical cell lysis occurs on time scales of several to tens of seconds and is adequate for analytes whose chemical states change on such time scales. However many biochemical processes within cells occur on sub-second to second time scales including the production of second messenger and signaling lipids or the activation of kinases and phosphatases.
...

Methods of Manufacturing Microdevices in Laminates, Lead Frames, Packages, and Printed Circuit Boards

Background: Microelectrical-mechanical systems (MEMS) are miniature mechanical devices intended to perform non-electronic functions such as sensing or actuation. These devices are typically built from silicon using lithographic techniques borrowed from the semiconductor industry. This manufacturing technique is expensive and limited. Furthermore, almost all micromachined devices must eventually be placed in a protective housing so that electrical connections can be made to the devices, and to p
...

Rescanning Laser Scanning Confocal Microscope

Background: The laser scanning confocal microscope (LSCM) is widely used as a confocal microscopy technique for imaging fluorescent objects in a manner that removes out of focus fluorescent light and thus provides increased axial resolution and better imaging compared to a standard fluorescence microscope. A laser is used since it can generate a very intense spot of light on the specimen. A problem with this detection method is that it requires measuring a rapidly changing low level of light an
...

Optimizing Sound to Suppress Tinnitus

Background: Tinnitus is the perception of sound in the absence of corresponding external sounds. Although known for its high-pitched ringing, tinnitus is an internal noise that varies in pitch and frequency. Tinnitus affects approximately 50 million people in the United States. Treatments such as drugs, surgeries and masking exist, but none are consistently effective and may have significant side effects. UC Irvine researchers have found that optimized sounds complementary to tinnitus can be us
...

Construction of Single Molecule Electronic Devices, with Applications to Biosensing

Background: "Molecular Electronics" (ME) generally refers to a class of novel electronic devices in which the electronic response and function are entirely controlled by single molecules. In principle, a wide range of devices can result from the incorporation of different types of molecules (e.g. molecules that switch, store charge, absorb light, or otherwise participate in reactions). Covalently linking a single molecule of interest between two electrical conductors enables the electrical inte
...

Prokineticin 2 and Prokineticin Receptor 2 as a Target to Treat Epilepsy and Seizures

Background: PK2 belongs to a family of secreted peptides that regulate diverse biological functions including serving as a regulatory molecule for circadian rhythms. The signaling of PK2 is mediated through two cognate G-protein coupled receptors Prokineticin Receptor 1 and Prokineticin Receptor 2 (PKR1 and PKR2). Technology: Researchers at the University of California have found that PK2 is an important regulator of seizure onset and epilepsy. A PKR2 modulator would thus help treat epilepti
...

Micromachined Passive Programmable Drug Delivery Systems

Background: The ability to deliver drugs locally to the site of need and over a prolonged period of time is important as a therapeutic method for many ailments and diseases. Many drugs are more effective if delivered at a specific site since they can be delivered in concentrated dosages at the point of interest, while maintaining an overall low dosage within the total body. Some drugs require delivery in places that are inconvenient for injection. For example, the highly invasive nature of the
...

Method and Apparatus for Performing Qualitative and Quantitative Analysis of Produce (Fruit, Vegetables) Using Spatially Structured Illumination

Background: The use of wide-field, broadband, spatially illuminated imaging of turbid media has the potential for simultaneous surface and sub-surface mapping of media structure, function and composition. This method can be applied with no contact to the medium over a large area and can be used in a variety of applications that require wide field image characterization. Technology: Scientists at the University of California, Irvine have developed a method that employs modulated imaging. This
...

High Sensitivity Optical Coherence Tomography

Background: Optical coherence tomography (OCT) is a non-invasive sub-surface optical imaging modality with high axial resolution and high signal to noise ratio compared to other imaging modalities such as ultrasound and MRI. Depending on the tissue's scattering and absorption, usually the imaging depth can be 1-2mm sub-surface in turbid tissue. A high sensitivity OCT system is desirable for better imaging depth and more imaging details. Technology: University of CA Researchers have developed
...

A New Mouse Model of Sjogren's Syndrome

Background: Sjogren's Syndrome is a grave autoimmune disease in which destruction of the exocrine glands leads to severe dryness of the eyes and mouth, with additional systemic complications. The pathogenesis of Sjogren's Syndrome is unclear, and treatment options are limited. Development of good animal models of the disease might lead to effective treatments for human patients, as well as a greater understanding of the cellular and molecular alterations that underlie disease progression. Tec
...

The Arhgap18 Gene and Schizophrenia

Background: Correlation of brain images and genotypes for diagnostics of neuropsychiatric disorders and drug discovery Technology: The invention includes general methods for identifying markers that correlate with neuropsychiatric disorders such as schizophrenia and bipolar disorder. The methods include measuring a brain image under different functional conditions, assigning statistical difference, and correlation of this statistical difference with genotype difference. Brain images may be p
...

Beta-Amyloid and Neurofibriallary Tangle Imaging Agents

Background: Positron emission tomography (PET) is a non-invasive test that helps doctors diagnose abnormalities, determine the extent of disease, prescribe treatment, and track progress. The patient is given a positron-emitting radiopharmaceutical and the PET scan locates and measures radioactivity, thereby distinguishing the "hot spots" for brain activity related to the specific radiotracer. Imaging agents using PET can greatly enhance chances of early diagnosis of Alzheimer's disease, whi
...

Dynamic Skin Cooling with Non-Toxic Cryogen with Minimal Global Warming Potential

Background: An important issue in laser treatment of cutaneous lesions is to protect the epidermis from thermal damage. This heating, which is primarily caused by light absorption in the melanosomes, can easily bring the temperatures of the basal layer above the threshold damage value of 65-70 degrees C. Pre-cooling of the epidermal basal layer from the ambient value of 35 degrees C to 0 degrees C increases the optical radiant exposure that can be safely delivered by a factor of two. Currently,
...

Restoring Cough Using Microstimulators

Background: Respiratory complications are the most common cause of morbidity and mortality following spinal cord injury. Patients with such injuries often suffer a disruption in the neuromuscular central nervous system control of the expiratory muscles. Such disruption or loss of control leads to direct loss of the ability to cough or produce an inner effective inner to clear airway secretions. This inability to cough or to clear airways results in respiratory tract infections and other related
...

Integrated Direct Electron Imaging Sensors

Background: Conventional charged particle imaging sensors use film and charged couple device (CCD). CCD has poor radiation tolerance and records images indirectly while the film is an analog recorder, unsuitable for high throughput digital imaging. What is needed then, is a direct, digital imaging sensor for high throughput digital imaging. Technology: University of CA researchers have developed a CMOS sensor dedicated to and optimized for direct imaging in electron microscopy. The invention
...

Gene Encoding CRAC Channel

Background: CRAC stands for Ca2+-Release Activated Ca2+. When Ca2+ from the endoplasmic reticulum (a major store of Ca2+) is depleted, a CRAC channel, is activated to slowly replenish the endoplasmic reticulum. The CRAC channel is critical to the activation of T lymphocytes, mast cells and other hematopoietic cells, as it provides the primary route for the influx of calcium into these cells. Inhibition of the CRAC channel could, therefore, provide one of the most direct means of modulating the
...

CAP/SORBS1 Gene and Diabetes

Background: Diabetes Mellitus (DM) is one of the leading causes of death in the United States (Bassett). The molecular defects underlying the development of most human Type 2 DM are not yet clear. But we know a combination of insulin resistance and islet cell failure brings on Type 2 DM. Insulin actions are mediated through insulin receptor. Recent transgenic studies have shown that defects in insulin receptor signaling may lead to both insulin resistance and islet cell dysfunction in experimen
...

Flexible Endoscopic Tube

Background: In performing, through an anatomical channel inside the body, a diagnostic or surgical procedure, the surgeon or operator may need to rotate with precision the distal end inside the body, from the proximal end outside the body. Precise transfer of angular rotation from the proximal to the distal end, without loss, or amplification, of angular rotation, would give the surgeon, or operator, the ability to orient the distal end in space inside the body. That way, they can rotate the in
...

Intragastric Balloon for Treatment of Obesity

Background: The BioEnterics Intragastric Balloon System™ is a non-surgical, non-pharmaceutical alternative for the treatment of obesity. The balloon system is designed to provide loss therapy to reduce health risks related to obesity or risks prior to vital supervised weight loss program. Endoscopically placed and inflated with saline, the balloon system (made of high-quality silicone) partially fills the stomach to induce the feeling of fullness and support patients in reducing food intake a
...

Microfluidic Device for Forming Monodisperse Lipoplexes

Background: The determinant factor for the successful applications of delivering drugs is to develop a non-viral and efficient carrier. Cationic lipid based liposomal carriers are the most attractive non-viral solution. Advantages of liposomal vectors include safety, lack of immunogenicity, ability to package large DNA molecules and ease of preparation. However, the conventional processes for catatonic lipids and DNA complex formulation are normally irreproducible. Technology: A microfluidic
...

Technique to Characterize NIitric Oxide Exchange in the Lungs

Background: It has been reported that the concentration of Nitric oxide (NO) in the exhaled breath was substantially elevated in patients with asthma who were not treated with corticosteroids, suggesting that exhaled NO was a potential noninvasive index of assessing lung and airway inflammation. Because NO exchange dynamics are significantly different from other well-studied gases such as carbon dioxide, new models and analytical methods are needed to understand the underlying physiology and ga
...

Methodology of Elasto-Mammography

Background: It has been well recognized that the tissue stiffness plays an important role for diagnosis of breast cancers, as tumors are stiffer than the surrounding breast tissues, and malignant tumors are much stiffer than benign ones. In other words, in vivo identification of the elastic moduli of normal and abnormal breast tissues, which describe the stiffness, should improve the accuracy for breast-cancer diagnosis. There have been elastography studies based on either ultrasound or MRI bre
...

Methodology of Elasto-Mammography

Background: It has been well recognized that the tissue stiffness plays an important role for diagnosis of breast cancers, as tumors are stiffer than the surrounding breast tissues, and malignant tumors are much stiffer than benign ones. In other words, in vivo identification of the elastic moduli of normal and abnormal breast tissues, which describe the stiffness, should improve the accuracy for breast-cancer diagnosis. There have been elastography studies based on either ultrasound or MRI bre
...

A Novel 7 Base Deletion in the Gene on Chromosome 21 for Diagnostic of Keratoconus

Background: Keratoconus is a noninflammatory progressive corneal thinning disorder leading to mixed myopic and irregular astigmatism and is implicated as a major cause of cornea transplants in the Western world. Genetic factors have been suggested as a cause of keratoconus. The stromal thinning of keratoconus corneas is related to increase in degradative enzyme activities. In addition, keratoconus corneas have an abnormal accumulation of cytotoxic byproducts. This may be due to alterations in t
...

Wafer Scale Glass Blowing

Background: Large scale confinement chambers have been created in the past using traditional glass-blowing techniques. However, conventional glass-blowing can only be used to create large components and requires the components to be made one at a time. Micro-glass spheres have previously been fabricated by letting glass particles fall through a temperature-controlled drop tower. While it is possible to create hollow spheres by introducing a blowing agent in the glass, these micro-spheres are no
...

An Integrated Microfluidic Platform with Lateral Dielectrophoresis (DEP) Based Cell/Particle Separation

Background: Most of the current techniques used for Dielectrophoresis separation use planar electrodes which sit at the bottom of the channel. With this design the electric field decreases dramatically as the height increases. Correspondingly, the gradient of the electric filed which is denoted as the direction of the DEP force will be along the height of the channel. This phenomena is implemented in field flow fraction (FFF) DEP to balance the gravity of the cells so that different cells will
...

Protein Nanocapsule for Drug Delivery

Technology: University of California researchers are investigating a protein scaffold system for molecular transport, namely, the E2 protein of Bacillus stearothermophilus in the pyruvate dehydrogenase complex. This model system has many features that make it attractive as a generalizable scaffold for drug delivery. Although it is a large complex, it self-assembles from smaller subunits that are easily heterologously expressed in E. coli. Since it is derived from a thermophilic organism, it is
...

Hybrid Binding Proteins Against Pathological Protein Aggregates in the Central

Background: The pathological protein aggregates such as beta-amyloids, alpha-synuclein and prion protein deposits in the CNS can be removed by vaccination, i.e., immunization using specific antibodies andlor synthesized antigens. Studies have demonstrated that clearance of these pathological protein aggregates in brain can attenuate brain cell loss and ameliorate brain function recovery. The disadvantage of existing methods is causing severe inflammatory response in host and involved in complic
...

Wafer-Level Micro-Glass Blowing

Background: Large scale confinement chambers have been created in the past using traditional glass-blowing techniques. However, conventional glass-blowing can only be used to create large components and requires the components to be made one at a time. Micro-glass spheres have previously been fabricated by letting glass particles fall through a temperature-controlled drop tower. While it is possible to create hollow spheres by introducing a blowing agent in the glass, these micro-spheres are no
...

Generation of Stable Concentration Gradients in 2D and 3D Environments Using a Microfluidic Ladder Chamber

Background: In the chemical, biomedical, and pharmaceutical industries, it has become increasingly desirable to perform large numbers of chemical operations in a highly parallel fashion. For example, cell culture methods are a commonly used research techniques that allows the systematic manipulation of a growth condition of cells. In cell culture the culture media and substrate can be varied under controlled conditions. With well known culturing techniques the entire cell is exposed to the same
...

Development of Diagnostics and Therapeutics for Cornelia de Lange Syndrome

Background: Cornelia de Lange Syndrome (CdLS) is a moderately common multisystem birth defects disorder that poses severe medical and social challenges for affected children and their families. Diagnosis of Cornelia de Lange Syndrome (CdLS) is based on clinical signs, including abnormal upper limbs, characteristic facial features, cardiac defects, cognitive retardation, and hearing loss. The syndrome varies widely in severity, and mildly affected cases may be difficult to diagnose. As many as h
...

Methods and Reagents for Screening New Drugs and Treating Ion Pump Associated Disorders

Background: Sodium/potassium ATPases (Na+/K+-ATPases), a family of multi-subunit ion pumps, are the most important active transporters in animal cells. They are required for maintaining the electrochemical gradient responsible for resting membrane potentials in neuronal cells and for the function of other transport proteins in a variety of cell types. The important regulatory activities of Na+/K+-ATPases make them an attractive therapeutic target for the treatment of neurodegenerative, cardiac,
...

Fabrication of Renewable RNA Microarrays and Methods

Background: Numerous methods and devices are available to detect and/or quantify target DNA in a test sample. In almost all of these methods, detection and/or quantification are performed utilizing a detectable label such as radiolabels, fluorescent and luminescent labels. Methodologies employing labels are costly and laborious. RNA microarrays for sensitive target DNA detection not requiring labels have been described. The major issue with creating RNA microarrays is that the attachment of pro
...

A Microfluidic Device for Enabling the Controlled Growth of Cells and Methods Relating to Same

Background: In many neurodegenerative diseases and in spinal cord injury, axons and synapses are damaged; a cell culture model is useful for investigation into these areas of research. In typical cell culture it is difficult to distinguish axon from dendrite, and fairly impossible to simulate microenvironments encountered along axons, dendrites or synapses. In a Petri dish for example there is no way to prevent cells from combining and hence it is difficult and in some cases impossible for even
...

Biomarker-Guided Prediction of Patient Adherence to Medications

Background: Adherence is one of the pivotal determinants of treatment outcomes for many medical disorders. It is estimated that 50% or more of patients with chronic conditions are noncompliant with medications at some time during their illness. Although there have been numerous attempts to develop approaches to evaluate adherence to drug therapy, including electronic dosing monitors, quantitative assessment of adherence remains a formidable challenge. Quantification of adherence to drug adminis
...

LEF-1 Internal Ribosome Entry Site

Background: Full length Lymphoid Enhancer Factor-1 (LEF-1) is a transcription factor that promotes cell growth via its interaction with the WNT signaling mediator beta-catenin. Truncated LEF-1 lacks the beta-catenin binding domain and opposes WNT signaling as a competitive inhibitor for WNT response elements. Numerous types of cancers derive from overactive WNT signaling. University of California researchers studied the post-transcriptional regulation of Lymphoid Enhancer Factor-1. Initiatio
...

Purification of Laminin 5

Background: Lamin-5, a protein produced by skin, mammary and other epithelial cells, may be involved in the progression of cancer. Purified human laminin-5 is not commercially available, and we have developed a purification procedure that may make it more available to the research community. Technology: The novel method method yields 1-2 mg of pure laminin 5. This process is unique because it produces pure, soluble non-denatured laminin-5. Application: The proposed purification is improve
...

Microfluidic Production of Monodispersed Submicron EmulsionsThrough Filtration and Sorting of Satellite Drops

Background: In the past decade, droplets have been intensively used by the industries as an agent for drug preparations, for plastic polymerizations, and chemical processing. Recent advancements in microfluidic droplet technology has enabled the precise sampling and processing of small volumes of fluids (picoliter to femtoliter) by the controlled viscous shearing in microchannels. Microfluidic technologies has transformed droplets to be used as liquid reaction vessels for screening protein crys
...

New Therapy to Inhibit Plasma Cells in Autoimmune Diseases

Background: In patients suffering from multiple sclerosis (MS) B cells, stimulated by axon antigens, infiltrate central nervous system. Technology: The inventors discovered a new class of antibodies that specifically binds to axons in early stage of acute lesions in multiple sclerosis (MS) brains. Further the inventors engineered a single chain-variable domain antibody fragment (scFv) from clonally expanded B cells from MS patients. This scFv antibody specifically binds to axons in acute
...

Medical Spectroscopy

DOMAIN Aging of injuries on a victim’s body is an important aspect of forensic medicine. Currently, visual assessment and colorimetry based on empirical criteria are the most common techniques for this task, although the results are uncertain. The aim of this study was to develop a mathematical model to describe blood diffusion within bruised skin, and to use this method to retrieve the age of a bruise from measured skin reflectance. DESCRIPTION NTNU researchers have developed a new method
...

Diagnosis and prognosis of cancers in GI tract

DOMAIN Although the incidence of gastric carcinoma (tumour in stomach) is declining, this disease is still the second most frequent cause of cancer death worldwide. Gastric carcinomas are often not detected until at an advanced stage; consequently, the 5-year survival rates are low and most often in the order of 10-20%. Variables such as size, microscopic differentiation and growth pattern, depth of infiltration as well as metastases in regional lymph nodes or in remote organs and tissues, all p
...

Alginate - Drug Delivery System

DOMAIN The mucosal surfaces of the body (such as GI tract, respiratory tract, eye, and genitourinary tract) constitute the major target for drug and gene delivery. As such, mucous secretions can have a significant influence on drug/gene complex uptake. These effects of mucus are usually more pronounced for larger drugs, such as protein molecules, particulates and colloidal systems, and for gene delivery complexes. DESCRIPTION In order to penetrate the mucus barrier, drug formulations may be m
...

Rapid prediction of gene expression levels using spectroscopy

DOMAIN The study of gene expression in cells is increasingly being used to understand their biological function. The genes are up- and down-regulated by a complex protein regulatory network, which responds to the biochemical changes originating from within and outside the cell. One of the most popular methods for performing gene expression studies of a large number of genes today is the micro-array technique. Expression is here measured through the amount of RNA strings produced for the differen
...

A Microfabrication Process for Vertical Microelectrodes inside Polymer Microchannels

Background: For microfluidic applications, different electrodes inside the microfluidic channels may be patterned by metal evaporation methods such as thermal evaporation, electric beam evaporation, and sputtering. Since most of the deposition methods only deposit very thin layers of metal (e.g., usually thousands of Angstrom), while the microfluidic channels have dimensions of 10-100um, this means the electrodes are often deposited at the bottom of the microchannel. Unfortunately, electrodes d
...

Wall-Less Microfluidics with On-Site Magnetohydrodynamic (MHD) Pumps for Continuous Flow Control

Background: A disadvantage of closed microfluidic channels are that they are made from different materials. Also, most of the microchannels are fabricated from lithography techniques which require the utilization of cleanroom environments, increasing the fabrication costs. Another disadvantage of the closed microchannel is that, after several rounds of fluid flow through, the channels become contaminated and difficult to clean. Technology: Researchers at the University of California have dev
...

Optimal selection of flurophores for bioimaging using hyperspectral cameras

DOMAIN In many situations it would be beneficial to have an instrument that can show In biology and medicine, fluorescent labelling is used to detect the location and concentration of various molecules. This can be used to discover how molecules are interacting in relation to diseases and biological functions. Often, it is of interest to study the distribution of multiple marker molecules in a tissue simultaneously. This, however, is made difficult by the fact that most fluorophore labelling met
...

Real time hyperspectral imaging

In many situations it would be beneficial to have an instrument that can show directly the spatial distribution of various chemical components in real time of a surface. Typical application areas are forensic and military where investigators can rapidly locate the presence of blood, gunpowder, toxic chemical and explosive residues on surfaces. To accomplish this, rapid (real-time) scanning is needed. In addition to that, the results have to be intuitively visualised. To detect the concentration
...

Fiber optic bundle based optical coherence tomography

Background: Optical coherence tomography (OCT) has been used for high resolution optical imaging in many areas of medicine, especially ophthalmology. "Conventional wisdom" was that OCT could not be done through flexible fiber bundles. Indeed, the use of flexible fiberoptic bundles to deliver OCT directly to a tissue sample has not been previously achieved. Technology: University of California researchers have developed a method and apparatus for performing fiber-optic bundle based OCT. Altho
...

ThirdEye – early detection of cerebral palsy (CP)

DOMAIN An early and accurate detection of cerebral palsy (CP) enables correct treatment at an early age to the infant at risk. Today’s methods for detecting CP are unable to establish a diagnosis until late in the first year of life, in some cases even later. Experts are currently doing analysis of infant spontaneous movements at 4 months of age. Opportunities exist for early and better diagnostic procedures. DESCRIPTION NTNU research scientists are developing a novel computer-based tool fo
...

Methods and Compostions for the Treatment of Immune Disorders and Regulation of Cytokine and Growth Factor Signaling

Categories: Pharmaceuticals: Central Nervous System Disorders, Immune and Inflammatory Disorders Biotechnology: Assays, Diagnostics, Model Systems Background: Mgat5 is an enzyme that glycosylates T-cell receptors to modulate T-cell activation and autoimmune response. Mgat5 Null PL/J Mice were developed as part of a study performed by scientists at Mount Sinai Hospital and the University of California, Irvine to establish an understanding of the role of Mgat5 modified glycans in the regula
...

MEMRI (manganese enhanced MRI)

DOMAIN Within an ageing population, ischemic heart disease (IHD) and secondary heart failure represent major challenges underlining a demand for diagnostic imaging to identify IHD in its different stages and forms. There is also a need to diagnose heart failure developing after myocardial infarction, to identify other forms of heart failure, and to monitor the efficacy of drug treatment. The seriousness and the epidemic nature of ischemic heart disease call for effective diagnostic methods to
...

Phage-displayed Peptide Library with Affinity for Bacterial Elongation Factor Tu

Background: The highly abundant GTP binding protein elongation factor Tu (EF-Tu) fulfills multiple roles in bacterial protein biosynthesis. EF-Tu also binds other ligands, including four structurally distinct families of antibiotics. The lack of sequence homology among the identified EF-Tu ligands demonstate promiscuous peptide binding by EF-Tu. Technology: A peptide library with a diversity of ~ 4.7X1011 different peptides was constructed containing both linear and disulfide-constrained pep
...

High Density Micromachined Electrode Arrays Usable for Auditory Nerve Implants and Related Methods

Background: Auditory prostheses using microelectrode arrays suffer from a number of limitations. Issues that were not resolved are electrode size, the need for electrical wires to communicate with and transfer power to the arrays, and the need for hand assembly of the devices. Technology: Researchers at the University of California have developed an auditory implant prosthesis consisting of a form-fitted microelectrode array and on-chip wireless circuitry. Key advantages of this invention in
...

Determining leakage through a heart valve

Determining the severity of a leakage through a heart valve is important. There are several parameters that are clinically interesting in this regard: the area of the lesion, the flow or flow rate through the lesion and the geometry of the lesion. These three parameters are not easy to measure. Current practices comprise mainly invasive techniques with catheters or non-invasive ultra- sound techniques. It is desirable to avoid invasive techniques, as they may be uncomfortable for the patient, an
...

Glycosylase enzymes

DOMAIN Glycosylases have found use in a number of methods aimed at analyzing DNA for the presence of altered nucleobases that could result from ionizing radiation or chemical oxidants, identifying mismatched base pairs, studying repair of abasic sites in double-stranded DNA and as a means to prevent carry- over contamination of PCR reactions by products from previously generated amplicons. Several different glycosylases have been identified, most of which are specific for a certain type of alter
...

Method and Apparatus of Using 3D Conductive Structures in Dielectrophoretic Separation Applications

Background: Researchers estimate that 80% of all machine failures are due to wear and that 1.3~1.6% of the GNP is lost to degradation of machinery due to friction-related wear. The abnormal abrasive wear due to lubricant contamination in marine diesel engines eclipses that of normal wear and the gap becomes wider with time. It was found that although oil filters used in automotive engines are designed to filter particles in the 15-30 µm range, particles with diameters below 10 µm caused 44% o
...

Rapid Bio-Molecules Detection by Adjacent Impedance Probing (AIP) Techniques

Background: Today, most detection schemes for DNA, proteins and other large biomolecules involve luminescent measurements using a variety of labels (i.e. fluorescent and/or chemiluminescent probes, photoproteins, etc.) and the binding of these large molecules to their complementary molecular structures (e.g., complimentary oligo-nucleotide hybridization and ligand binding protein or antibody binding). While spectroscopic methods afford the necessary sensitivity for most of these measurements, t
...

Fabrication of Suspended Carbon Micro and Nano-scale Structures

Recent attention has focused on high aspect ratio carbon micro-electromechanical (C-MEMS) because of the many applications possible, such as micro-electrodes in electromechanical sensors and miniaturized energy storage/energy conversion devices. Further, suspended micro/nano carbon structures exhibit a wide electrochemical stability window which makes them interesting for integration in mechanical, electrical, and electromechanical measurements. One of the biggest advantages of suspended micro/n
...

Method to Characterize Exhaled Nitric Oxide from the Airways Using a Sequence of Breathhold Maneuvers

Background: Exhaled nitric oxide (NO) arises from both airway and alveolar regions of the lungs, which provides an opportunity to characterize region-specific inflammation. Current methodologies rely on vital capacity breathing maneuvers and controlled exhalation flow rates, which can be difficult to perform, especially for young children and individuals with compromised lung function. In addition, recent theoretical and experimental studies demonstrate that gas-phase axial diffusion of NO has
...

Total Ankle Arthroplasty

Background: Total ankle arthroplasty (TAA) is the process of replacing a diseased ankle with a prosthetic ankle. Over the years there have been several efforts to provide a total ankle replacement solution. However, all such current solutions have significant drawbacks, including complications relating to aseptic loosening, delayed wound healing, wound dehiscence, and prosthetic subsidence. Other problems include prosthesis migration and loosening, and osteolysis at the tibial component. One com
...

Methods for Assessing the Temperature of Deep Tissue in vivo Using Non-ionizing Radiation

Background: Measuring temperature in tissue is highly relevant to treatments applied to tissue in which the tissue becomes heated. This includes methods that treat tissue with RF (Radio Frequency) such as radio frequency ablation of cancer, hyperthermia treatments of cancers and cosmetic skin treatments. Monitoring the instrument being used to heat tissue allows the instrument to adjust to the energy being deposited in the tissue and increase or decrease the tissue temperature. Current methods
...

Microscope Immersion Fluid Applicator

Background: Microscopy is an important tool used by all researchers in the scientific community. Often, a microscope user will first scan the specimen with a low power dry objective and then wish to switch to an oil, water or glycerin immersion objective to increase optical resolution. In other cases, a user might want to scan a large area, i.e. a multiple-well plate, and would need to replace the immersion media as it is sheared away from the objective lens. In the case of inverted microscopy,
...

A New Tandem-Affinity Tag for Two-Step Protein Purification under Fully Denaturing Conditions

Background: Preservation of posttranslational modifications during purification is crucial for successful mass spectrometric analyses of protein modifications. Current tandem-affinity purification strategies require native conditions and are therefore susceptible to loss of posttranslational modifications during cell lysis and purification because modifying as well as de-modifying enzymes remain active under these conditions. Technology: University of California, Irvine researchers have deve
...

Transgenic Mouse Model for Screening Analgesic Agents

Background: UC Case No.: 2004-692 Patent Application Publication No.: WO/2006/017293 International Application No.: PCT/US2005/024697 This invention is an animal model and various cell-based models to determine nociception, pain transduction, and pain threshold. Researchers at the University of California, Irvine, discovered that overexpression of voltage-gated calcium channel alpha-2-delta-l subunit in neural tissue, and especially increased expression in spinal cord and dorsal root gan
...

New Polymeric Biomaterials

Technology: The invention is on new polymeric biomaterials. The new biomaterials were created by chemical synthesis with carbohydrates and amino acids as building blocks. The biopolymers have a specific alternating structure between carbohydrate and peptide units. Application: The new biomaterials can also be used for many other biomedical and biotechnology applications such as: implants, prosthesis, tissue engineering, drug delivery, sensors, substrates for enzyme-linked immunosorbent assay
...

Cell Encapsulation on a Microfluidic Platform

Background: Cell encapsulation is a highly useful tool in cell culturing, assay, and cell-based therapy applications. Encapsulation has traditionally been accomplished by extrusion through a nozzle, forming an air/water emulsion, into a bath containing a polymerizing agent. However, this batch processing technique is characterized by its inability to trap cell droplets before or without polymerization and non-uniform polymerization times across droplet population. Furthermore, minimum droplet s
...

A Diffusive Probe for Quantification of Optical Properties of Superficial Layers

Background: Diffuse optical spectroscopy (DOS) has been successfully employed for quantitative determination of in vivo optical properties and chromophore concentrations of deep tissues such as breast and brain. This invention is a method and probe design for obtaining quantitative optical properties and chromophore concentrations of tissue components "in-vivo" at superficial depths and "short" source detector separations. This domain, in which the source and detector are in relatively close pr
...

Use of Laser-Induced Fluorescence for Determination of Root Canal Pathology and Localization of Pathological Tissues to Facilitate Endodontic Therapy

Background: Currently endodontic therapy is based upon radiographic information and tactile feedback. Direct detection and localization of infected dentin and pupal tissue has not been possible. It has been determined that when excited at appropriate wavelengths of light, the fluorescence emission of healthy dentin, decayed dentin, bone, vital pulp, non-vital pulp, and pus have unique spectra. Some endodontic organisms can also be detected using this approach. Technology: Researchers at the
...

Nrf1 Deficient Mice as a Model for Liver Cancer and Non-Alcoholic Steatohepatitis

Background: Antioxidant and xenobiotic metabolizing enzymes are critical in the protection against chemically induced oxidative/electrophilic stress in cells that cause damage, DNA mutation, apoptosis, and cancer. Transcription of these cytoprotective genes and xenobiotic metabolizing genes is regulated through cis-active sequences known as antioxidant response elements (ARE). Coordinated induction of these genes mediated through the ARE is regulated by a signal transduction system that is stil
...

Invention of New Dendritic Amphiphiles

Background: Amphiphilic compounds are of general interest in numerous disciplines in life sciences, especially in pharmacology and general chemistry, and numerous amphiphilic compounds are known in the art. However, traditional amphiphilic compounds usually forms micelles or aggregates that are often not stable, or tend to interfere with the integrity of reagents that are mixed with such compounds. Moreover, many of the well known amphiphilic compounds have further disadvantages, including limi
...

Regulated Growth Factor Delivery for Nerve Regeneration

Background: Options for successful repair of peripheral nerves after traumatic injury remain suboptimal. Current strategies are limited to transfer of donor nerves from functional areas, with substantial donor-site morbidity and variable outcomes. The development of tissue-engineering strategies offers significant hope to patients facing functional impairment and cosmetic problems after such injuries. Tissue-engineered constructs used to bridge nerve gaps have the potential to improve options f
...

Automatic Detection and Diagnostics of Diabetic Retinopathy

Background: Diabetes is a condition that affects blood vessels throughout the body, particularly in the kidneys and eyes. Diabetic retinopathy is a complication of diabetes and is the leading cause of new blindness in the United States. Diabetic retinopathy results when diabetes affects vessels in the eyes, producing abnormalities such as microaneurysms and hemorrhages. These abnormalities are the same color as that of blood vessels, causing some areas of the normal blood vessel system in the r
...

A Microfluidic Dynamic Vapor Control System

Background: Controlling the chemistry of small drops is troublesome owing to the difficulty in handling and metering small volumes. To change the chemistry of a 50nl drop by 10% would require adding or removing a 5nl quantity - very difficult. As a result, most nanovolume assays are set up once and never modified afterwards. Large environmental systems have been built to provide vapor controlled change of chemistry. However, these are far too bulky and inconvenient to be of use to a typical res
...

Polymeric Valves for Controlled Drug Delivery

Background: Biochips can be used in vitro or in vivo to deliver small, controlled amounts of chemicals or other molecules at controlled times and rates. Current delivery release mechanisms include electrochemical dissolution of thin anode membranes covering microreservoirs filled with drug. The metal valves, which are irreversible, consist of thin suspended non-porous layers that can be electrochemically dissolved or disintegrated by water electrolysis. Another mechanism involves sphincter conf
...

Methods for Treating Port Wine Stain Birth Marks, Hemangiomas and Other Conditions Caused By Aberrant Blood Vessels

Background: Cutaneous vascular lesions including port wine stains (PWS) and hemangiomas are frequently treated with laser and light devices. The pulsed dye laser (PDL) is the current standard means of treatment care for PWS and achieves effect by selectively targeting tissue vasculature. However, few PWS patients (< 20%) achieve complete blanching of their lesion and multiple treatments (5 -30 or more) are generally required. One primary factor limiting vascular lesion blanching for many patien
...

Neuropeptide S (NPS) as a Treatment for Anxiety, Sleep Disorders, Attention Deficit Hyperactivity Disorder, Attention Deficit Disorder, and Asthma

Background: Patent "WO02/31145" discloses a newly deorphanized GPCR system, Neuropeptide S (NPS), the endogenous ligand, and its cognate GPCR. However this patent does not detail the pharmacological or physiological function of NPS and its GPCR. University of California, Irvine researchers have characterized NPS's function in the CNS. Technology: University of California, Irvine researchers have shown that central administration of NPS increases locomotor activity in mice and decreases parad
...

Method for Quantitative Digital Color Imaging of Objects

Background: In many disciplines, quantitative measurements of color are required to evaluate nondestructively the state of an object (e.g., quality of produce). This characterization is typically performed using contact point measurement devices. A limitation of these devices is that multiple measurements are required to characterize an entire object; if multiple objects must be characterized, then this process may be time consuming. Furthermore, these devices interrogate both superficial and d
...

High-Isolation Tunable MEMS Capacitive Switch

Background: For a typical MEMS switch, the resonant frequency is around 20 to 30 GHz which can not be employed by commercial wireless systems operating at a frequency band of 2 to 8 GHz. This resonant frequency can be tuned by changing the inductance of the switch membrane. When inductance is increased, the resonant frequency is moved to a lower frequency band at which location, high isolation is achieved. There have been many published solutions to this problem but it has been found to be diff
...

Reagents and Methods for Diagnosis of Attention Deficit Hyperactivity Disorder

Background: Attention Deficit Hyperactivity Disorder (ADHD) is the most prevalent disorder of early childhood. It affects an estimated 3-6% of elementary school children. ADHD consists of developmentally inappropriate inattention, impulsivity, and hyperactivity with early onset (before the age of 7). Evidence of a strong genetic component of ADHD has come from a variety of twin, adoption, and family studies. Candidate gene studies have documented an association of ADHD with the dopamine rec
...

Microfluidic Devices for Controlled Viscous Shearing and Formation of Amphiphilic Vesicles

Background: Amphiphilic vesicles are artificial cells with applications in drug delivery (including biomolecular nanomedicine such as DNA, peptides, proteins), combinatorial chemistry, nanoscale chemical reaction chambers, biomolecular devices (power, optical, electrical), and various biosensors. Technology: Researchers at the University of California, Irvine, have developed a microfluidic device (i.e. ‘Lab-on-a-chip’) that controls viscous shearing of oil-water droplets on a micro- and
...

An Antibody-based Method for Reducing Ocular Inflammation which Can Aid in Corneal Transplantation

HSV keratinitis is characterized, in part, by scarring of the eye as a result of HSV reactivation. To date, corneal transplantation is the standard treatment method. A significant obstacle preventing successful corneal transplantation is inflammation that occurs within the eye (ocular inflammation). Researchers at University of California, Irvine and the University of Oklahoma have identified an antibody that prolongs survival in HSV infected mice and reduces inflammation within the eyes of infe
...

A New Burr Design

Background: One of the great limitations in spine surgery is the difficulty with removing bone without damaging adjacent soft structures (such as the spinal cord, nerves, and durra). This difficulty has significantly restricted the ability to do effective endoscopic surgery around the spine. Shielding of the cutting tool makes the tool too large to work in the limited space given, and makes the tool too dangerous and inefficient. Using any of the current cutting tools without complete visualiza
...

New Clinical Bymixer for Fast-Response, Adjustable Measurement of Mixed Expired Gas Fractions in the Anesthesia Circle Circuit

Background: The volume of O2 consumption (VO2) and the volume of CO2 production (VCO2) can give vital information about a patient under anesthesia. For example, monitoring VO2 can detect non-steady state critical events and changes in tissue metabolism in anesthetized patients. However, VO2 and VCO2 are typically NOT measured during anesthesia because collection of mixed expired gas is nearly impossible in the circle circuit. Instead, an inline bypass mixing chamber, or bymixer measures mixed V
...

Novel, Stand-alone, Closed Ventilating Circle Circuit with CO2 Absorption and Sensitive Spirometry Measures Pulmonary O2 Uptake During Anesthesia

Background: The volume of O2 consumption (VO2) and the volume of CO2 production (VCO2) can give vital information about a patient under anesthesia. For example, monitoring VO2 can detect non-steady state critical events and changes in tissue metabolism in anesthetized patients. However, no accurate calibrating measurement of VO2 is available during anesthesia. Technology: University of California, Irvine researchers have developed a novel, closed circle, ventilating circuit which can be a ca
...

High-Resolution X-Ray Crystal Structures of Tritrichomonas Foetus Inosine Monophosphate Dehydrogenase as Lead Compound for Structure-Based Drug Design

Background: Trichomoniasis is a venereal disease of cattle caused by a protozoan parasite, Tritrichomonas foetus. The disease has been reported worldwide with an estimated economic loss to the U.S. beef industry exceeding $100 million annually due to reduced conception rates, lowered weaning weights and increased culling. Currently there is no treatment for T. foetus. Technology: University of California, Irvine researchers utilized high-resolution x-ray crystallography to study the structur
...

A Polymeric Micro-Cantilever Resonator Array and Its Implications in Auditory Prostheses

Background: Ten percent of the general population suffers from hearing loss and that number rises to thirty five percent for people over sixty-five years of age. However, current digital hearing aids and cochlear implants to ameliorate hearing loss have their disadvantages. Some of these include high power consumption and long processing time associated with a large number of channels which increases its cost and size and limits its utility. Technology: University of California, Irvine resea
...

Selective Inhibition of Enzyme Isoforms of Nitric Oxide Synthetase

University of California, Irvine researchers have discovered a method to design and use selective inhibitors against a family of nitric oxide synthetase isoforms that control the production of nitric oxide (NO). X-ray crystallography was utilized to generate isoform specific NOS-inhibitor complexes with unique structural characteristics. An overproduction of NO has been linked to several diseases, conditions and addictions, including: ischemic injury and other brain damage after stroke, migraine
...

Methods for Assessing the Condition of Bone In-Vivo Using Non-Ionizing Radiation

Background: The ability to assess the health and condition of bone tissue is important since bone tissue viability is closely linked to composition, structure, and physiology. Traditional methods for assessing the condition of bone involve the use of X-rays (via electron density) and ultrasound (via tissue density). Several methods are available to measure bone density, the most widely used technique is DEXA (Dual Energy X-ray Absorptiometry). Newer techniques such as ultrasound appear to be a
...

Vascularized Artificial Tissue

Background: There exists a need for thick artificial tissues to cover large tissue defects following trauma or surgical resection of tumor. The primary limitation in the design of these tissues as well as other complex thick tissues is a vascular supply to deliver oxygen and essential nutrients. Technology: University researchers have developed a technique to prevascularize a thick tissue in vitro using a biodegradable microfluidic network, for subsequent implant. The ultimate shape of the t
...

hKCa3/KCNN3 Calcium Activated Potassium Channel: a Diagnostic Marker and Therapeutic Target for Heritable, Neurological and Psychiatric Diseases

University of California, Irvine researchers have discovered SKCa3-1b and SKCa3-1c, two novel isoform variants of the SK3 (also known as KCNN3) gene. The SK3 gene is located on human chromosome 1q21 in a region containing a major susceptibility locus for familial schizophrenia and familial hemiplegic migraine associated with permanent cerebellar ataxia. Researchers have also found that these two novel isoforms dominantly-negatively suppress SKCa3-1A currents. Suppressing SKCa3-1A currents create
...

Novel Cytodiagnostic Markers for Cancer

Background: Cervical cancer is one of the leading causes of cancer-related death in women worldwide. Approximately 500,000 new cases are diagnosed each year and pre-cancerous abnormalities of the cervix occur in far greater numbers of women each year. Cervical carcinomas develop progressively from hyperplastic tissue changes over the course of years or even decades. The molecular mechanisms that regulate the induction and progression of neoplasias to cancer are poorly understood and supports th
...

Wnt, Fz, and BMP-6 Receptors for the Treatment and Detection of Colon Cancer

Background: Ectopic activation of the Wnt signaling pathway leads to increased cellular growth and division in experimental organisms and mutations in the Wnt pathway. Wnt pathway genes are tightly linked to the genesis of certain cancers in humans, such as colon cancer. Technology: University of California, Irvine, researchers have found that factors of the Wnt signaling pathway are associated with colon carcinogenesis and may therefore be useful for prognosis, diagnosis or treatment of col
...

Platelet Aggregation Inhibitors

Thrombin is an enzyme in the blood that plays a key role in platelet formation during injury. While blood coagulation is essential for a surface wound, platelet activation underlies various pathological situations such as unstable angina pectoris, myocardial infarction and stroke. Thrombin is mediated by protease activated receptor-1 (PAR-1) which is expressed in the nervous system and in platelets. Once activated by thrombin, PAR-1 induces rapid and dramatic changes in cell morphology that is c
...

ADP Glucose Receptor as a Target for Disorders Involving Platelet Aggregation

Background: Recently, activation of the P2Y12 G-protein coupled receptor (GPCRs) has been shown to be central to platelet aggregation. Drugs preventing platelet aggregation are being tested, but one that would be specific to the P2Y12 receptor would capture a large market share. Developing drugs for the P1Y12 receptor is difficult, because it is a receptor that is naturally activated by ADP. Since practically every cell expresses ADP-activatable receptors, developing a drug screening program di
...

A New Process of Adding Alkyl Groups to Organic Substrates Using TmI2(MeOCH2CH2OMe)3

Background: In the pharmaceutical industry, synthetic chemists often alkylate starting compounds to generate compounds that have more desirable properties. However, the current reagents used in this modification process can be expensive or harmful to the chemist. Technology: Researchers at the University of California, Irvine, have discovered a compound that allows a variety of important chemical transformations to be accomplished using cheaper materials and eliminates the use of some carcin
...

Use of Infectious and Pathogenic Clone of a Lung Cancer-Inducing Retrovirus for Generation of Therapeutic Reagents and Diagnostic Tests

Background: Jaagsiekte sheep retrovirus (JSRV) causes broncheolo-alveolar carcinoma in sheep and is a significant veterinary problem world-wide. Clinically and histopathologically, this disease has strong resemblance to broncheolo-alveolar carcinoma (BAC) in humans which accounts for approximately 25% of human lung cancers. Technology: Researchers at the University of California, Irvine have isolated the first infectious molecular clone of JSRV and have developed techniques for production of
...

Donor Organ Preservation for Transplantation Using a Hemoglobin Based Solution

During heart transplantation, the heart must be preserved while it is transported from donor to recipient. Standard techniques call for the heart to be immersed and cooled in cardioplegia or saline solution. Unfortunately, this method does not deliver necessary oxygen, nutrients, and electrolytes to the heart. Newer methods of preservation have focused on creating blood-like substitutes. At the University of California, Irvine, researchers have formulated a solution that significantly lengthens
...

Modulation of Cell Growth and Growth Factor Signaling Through Removal of Glypicans

Background: Membrane associated heparin sulfate proteoglycans (HSPGs) have been shown to play important roles in many aspects of cell behavior, including cell-cell and cell-extracellular matrix adhesion and growth factor signaling. Glypicans, members of the HSPGs, are a family of polypeptides that appear to carry the majority of the heparin sulfate on mammalian cells. These glypicans are attached to the plasma membrane via glycosylphophatidylinositol (GPI) anchors. Technology: Glycosylphosph
...

Novel and Selective Inhibitors of HIV-1 Integrase

In the ongoing battle against the human immunodeficiency virus (HIV), researchers at the University of California, Irvine, have synthesized novel compounds that inhibit the protein HIV Integrase and prevent the virus from replicating, thus providing an alternative to currently available AIDS therapy. Current therapies target the HIV proteins Reverse Transcriptase and Protease, but both these therapies have toxic side effects. Application: These novel, proprietary compounds are potent HIV Inte
...

Diagnostic Marker and Novel Therapeutic Target for Neurodegenerative Disorders

Scientists at the University of California, Irvine, have isolated a candidate gene that may be responsible for increasing the risk of schizophrenia and bipolar disorder. This elusive gene encodes for a potassium ion channel that acts like an "off switch" by dampening electrical activity in neurons. It has been known that street drugs such as PCP create schizophrenia-like symptoms by blocking NMDA receptors in neurons. Over-activity of this potassium ion channel blocks the same NMDA receptors, wh
...

Enhancement of Host Defense Via Receptor(s) for C1q

Background: As the development of protein products and peptide mimetics becomes more prevalent in the biotechnology and pharmaceutical industries, the ability to mimic the protective and modulate the inflammatory effects of the recognition protein of the classical complement cascade in blood and body fluids will increase. Technology: Researchers of the University of California, Irvine, have discovered and sequenced a novel receptor molecule which can be used to develop agonists that will pro
...

Humidity and Temperature Sensor Device for the Determination of Oxygen Consumption in Medical Applications

Background: The determination of oxygen consumption is an important monitor of normal cardiopulmonary and tissue function in anesthesia and critical care medicine. This requires accurate measurement of inhaled and exhaled volumes. Traditionally, it has been very difficult to measure the extra volume of exhalant (compared to inhalant) because of the difficulty in measuring the extra warmth and humidity of exhaled air. If it were possible to continuously and immediately measure temperature and hu
...

A New Polymerization Method for Polymethylene

Background: The most common method for manufacturing polyolefins and their derivatives is by polymerization of olefin monomers with Ziegler-Natta catalysts or by the use of free radical, nucleophilic, or electrophilic initiators. Although one can achieve high molecular weights with these methods, the resulting products are often polydisperse. Many types of polymers are very difficult, if not impossible to manufacture by olefin polymerization. Technology: Researchers at the University of Cali
...

Transgenic Animal Models for Alzheimer's Disease

The present invention provides non-human, transgenic animals for use as a model for the study of Alzheimer's Disease. The animals exhibit pathology associated with Alzheimer's Disease. The phenotype is conferred in the animals by the introduction of an amyloid-containing polypeptide fragment of amyloid precursor protein, through the introduction of the DNA into the germ and somatic cells of the animal by transgenic methods.
...

Isolation of Biological Materials

Currently, flow cytometry is the only method available for the purification of specific chromosomes. The current flow cytometry method requires a long time to sort large quantities of cellular starting material for a single chromosome. Furthermore, species with similarly sized chromosomes, such as the mouse, make separating individual chromosomes by DNA content alone often impossible. Also, the debris and crosscontamination in sorted preparations can compromise their final utility. Technol
...

Method of Inhibiting the Transport of L-glutamate

Background: L-Glutamate is one of the most abundant excitatory neurotransmitters in the mammalian brain and, as such, is responsible for many synaptic transmissions in the central nervous system. Yet, while other neurotransmitters are degraded rapidly to terminate the transmitter signal, L-glutamate is removed from the synaptic cleft by high affinity transport. Furthermore, little is known about the conformational requirements of substrate binding to this transport system. Technology: Resear
...

Assay for and Treatment of Autoimmune Disease

The invention is directed to prognosis, diagnosis and treatment of autoimmune diseases, including SLE (systemic lupus erythematosus), type-1 diabetes, experimental allergic encephalomyelitis (EAE) and other, which demonstrate uniquely the presence abnormal double-negative (CD4- and CD8-) T cells expressing large numbers of type 1 K+ ion channels. Such channel expression parallels the ability to induce autoimmunity and therefore the invention is directed to an assay for, and consequent method of
...

Position-Specific Insertion Vectors and Method of Using Same

Background: Retrovirus vectors infect cells at high efficiency and are capable of integrating DNA copies of themselves into a host genome. Such vectors are, in many ways, desirable for introduction and expression of exogenous DNA sequences in animal cells. In gene therapy or in the production of transgenic animals, prolonged, stable expression of exogenously introduced genes is a requirement in order for the treatment to become effective. This requirement stems from certain pitfalls or ineffici
...

Biomimetic Agents for Replacement of NAD+/NADH in Enzymatic Reactions

APPLICATION OF TECHNOLOGY: - Preparation of chiral, pharmaceuticals, or specialty organic compounds - Biosensor technology for medical, chemical and biological sensing ADVANTAGES: - Co-factors are stable in air and water - Co-factors are designed without sugar, phosphate, or adenine groups - Co-factors can be used with a system that prevents co-factor loss downstream - The technology enables cost-effective co-factor regeneration ABSTRACT: Richard H. Fish, John B. Kerr, and H. Christine
...

Berkeley Ergonomic Arm

Berkeley Ergonomic Arm is a practical ergonomic intervention and support system to improve productivity and reduce work place ergonomic-related injuries. Berkeley Ergonomic Arm dynamically enhances musculoskeletal support and comfort during sitting tasks at the computer or standing at a bench top or manufacturing station. The aim is to reduce the likelihood of cumulative trauma injuries to the upper extremities. Business Opportunity: This is a ground floor opportunity with enormous upside ma
...

Apolipoprotein A-1 Variants for Reducing Cardiovascular-Related Risks

Single dose drug therapy for preventing restenosis and reducing the effects of acute phase response ADVANTAGES: - Promises to help prevent restenosis and thereby reduce the frequency of repeated invasive procedures to clear arterial blockages - Potentially inhibits the harmful oxidative effects of acute phase response - Increases paraoxonase activity by up to 30% ABSTRACT: Trudy Forte and Michael Oda have developed functional apolipoprotein A-1 (apoA-1) mutant proteins and their encoding
...

Antibodies for Monitoring and Phosphorylation Sites for Inhibiting DNA Double-Strand Break Repair

APPLICATIONS OF TECHNOLOGY: - Developing therapeutics for the radiosensitization of cancer cells - Assessing the efficacy of cancer treatments - Cancer drug screening and development - Research on the in vivo activity of DNA-PK ADVANTAGES: - Identifies two major DNA-PKcs autophosphorylation sites which are involved in DNA double strand break repair - First technology to enable the in vivo monitoring of DNA-PK response to DNA damage - Offers an antibody for drug screening and development
...

An Apolipoprotein that Strongly Modulates Plasma Triglyceride Levels

APPLICATIONS OF TECHNOLOGY: - Creation of new lipid-reducing drugs - Large scale genetic screening of individuals for cardiovascular disease susceptibility - Delivery of the ApoAV protein to reduce high triglyceride and VLDL levels in humans - Gene therapy potential for this liver-specific gene - Direct plasma administration of the protein - Examination of constructed mouse lines to study ApoAV’s function and to test therapies. - Transgenic mice have 70% reduced triglycerides - Knockout mice
...

Aligned Crystal Growth at Polymerized Membranes

APPLICATIONS OF TECHNOLOGY: - Semiconductor crystals - Magnetic materials ADVANTAGES: Low-cost, low-energy method works at room temperature ABSTRACT: LBNL researchers, Deborah Charych and Amir Berman, have developed a low cost, low energy method of growing oriented, co-aligned crystalline materials under aqueous, room temperature conditions. The low temperature method uses a highly oriented polymer that serves as a 'template' for growth of inorganic crystals. The crystals grown on this
...

Assays Based on the Role of Dystroglycan and Alpha-Dystroglycan Proteolysis in Tumor Cell Growth

PATENT SUMMARY: The present invention relates to methods and compositions for the diagnosis and treatment of cells lacking normal growth arresting characteristics. This characteristic is referred to as "tumorigenicity," which means the properties of a cell normally associated with tumor forming properties, especially, growth arresting properties, normal cell arrest, and appearance in the 3D-BM assay. Normal, non-tumorigenic cells will be polarized and, in the case of mammary epithelial cells, f
...

Using Novel Enzymes to Produce Advanced Biofuels

APPLICATIONS OF TECHNOLOGY: Biosynthesis of carboxylic acids and lactones for biofuel production ADVANTAGES: - Provides biofuel alternatives to ethanol - The new enzymes can be modified to tailor-make a wide variety of lactones and carboxylic acids - Controlled placement of ester linkages may result in enhanced combustion properties - Esters and lactones are more hydrophobic than their corresponding alcohols, offering compatibility with the current fuel infrastructure - Esters and lactones
...

Low-resistivity Photon-transparent Window Attached to Photo-sensitive Silicon Detector

AVAILABLE TECHNOLOGIES Low-resistivity Photon-transparent Window Attached to Photo-sensitive Silicon Detector APPLICATION OF TECHNOLOGY: - Medical Imaging - Other Digital Scanning technologies ADVANTAGES: - Inexpensive and efficient - High quantum efficiency without thinning - Backlighting in the blue wavelenths; improved red wavelength performance - Ease of fabrication Stephen Holland at Berkeley Lab has developed an invention that comprises a combination of a low resistivity, or
...

High Quantum Efficiency Charge-Coupled Device

APPLICATIONS OF TECHNOLOGY: Low-level light imaging (Astronomical research, Raman Spectroscopy) ADVANTAGES: - Back illuminated - High near-IR quantum efficiency without fringing - Excellent broadband wavelength response - Fully depleted for improved spatial resolution - Simplified fabrication - Superior radiation hardness ABSTRACT: Stephen Holland at Berkeley Lab has developed a p-channel CCD with high quantum efficiency in the blue and near infrared wavelengths by combining a high-resi
...

Genes Encoding Telomere-Associated Proteins

APPLICATIONS OF TECHNOLOGY: - Molecular biology research - Medical research ABSTRACT: Telomeres are believed to be critical regulators of the genome, and telomere stability important for preventing cancer and ageing. Regulation of telomere length is believed to be controlled by multiple telomere-associated proteins. Using a previously cloned human telomere binding protein, Berkeley Lab researchers Sahn-ho Kim and Judith Campisi cloned two novel human telomere-associated proteins, TANK-2 and
...

Full Karyotype Analysis for Increasing Pregnancy Rates per IVF Cycle

APPLICATIONS OF TECHNOLOGY: - Screening all 24 chromosomes for numeric chromosome abnormalities during Preimplantation Genetic Diagnosis - Diagnosing disease states requiring cytogenetic analysis - Monitoring the effects of accidental or occupational exposure to environmental hazards ADVANTAGES: - Allows full karyotype analysis of cells in interphase - Highly adaptable and flexible assay design - Works with a variety of cell types EXPECTED OUTCOMES: - Fewer spontaneous abortions - Fewe
...

Fast Scintillation Detectors Using Direct-Gap Semiconductors

APPLICATION OF TECHNOLOGY: - Detection of ionizing radiation (e.g. x-rays, gamma rays, neutrons) - Nuclear materials detection - Nondestructive materials evaluation - Nuclear medical imaging - Electromagnetic shower counters for high energy physics and astrophysics - Oil well logging ADVANTAGES: - Doping strategy is designed to transform a direct-gap semiconductor into a fast, luminous scintillator - The CdS(In, Te) scintillator has good luminosity and a response time of 3 ns, 10 to 100 ti
...

Comprehensive Prognostic Markers and Therapeutic Targets for Drug-Resistant Breast Cancers

APPLICATIONS OF TECHNOLOGY: Under a microscope, fluorescent markers (right) reveal amplified genes in the chromosomes of a breast tumor’s cells. - Identifying patients with breast cancers that are resistant to conventional chemotherapies and developing new treatment strategies for them - Screening patients with chemo-resistant tumors for clinical trials - Using gene-specific inhibitors to treat chemo-resistant breast cancers - Researching cancer progression and remission ADVANTAGES: -
...

Color and Shape Changing Polymeric Ribbons and Sheets

PATENT APPLICATION ABSTRACT: The present invention provides the design, synthesis and characterization of compositions comprising asymmetric bolaamphiphilic lipids that form extended polymeric ribbons and wide sheets. These compositions may be doped, or interspersed, with various compounds to fine-tune the fluidity and rigidity of the bolaamphiphilic lipid composition, and promote other morphologies of the composition, including fluid vesicles and truncated flat sheets. Upon an increase in pH t
...

Electrospray Emitters for Integrating Mass Spectrometry with Microfluidic Devices

APPLICATIONS OF TECHNOLOGY: - Multinozzle electrospray emitters for proteome-on-a-chip - Mass spectroscopy of peptides and biomolecules with a wide range of weights - Process for fabricating complex silicon-based bioanalytical tools ADVANTAGES: - First monolithic interface between mass spectrometry and silicon based microfluidic technologies - Can be massed produced from silicon wafers - Promises to be up to 20 times less expensive than current state-of-the-art tips - Stability and sens
...

Termite Microbial Enzymes for Energy Efficient Biofuel Production

APPLICATIONS OF TECHNOLOGY: Converting plant lignocellulosic biomass to simple sugars for fermentation into biofuels, and hydrogen. ADVANTAGES: - Promises to be more energy and chemically efficient than the fungal enzymes currently in use - Combinations of the enzymes could be optimized for a particular feedstock ABSTRACT: Researchers at Berkeley Lab’s Joint Genome Institute, California Institute of Technology, and Verenium Corporation have discovered and sequenced over 300 microbes i
...

High Efficiency Micro-Scale Light-Concentrating Arrays for Digital Imaging

IB-2279 APPLICATIONS OF TECHNOLOGY: • Digital imaging • Digital cameras, image scanners, and pixelated detectors • Applicable to visible, ultraviolet and infrared cameras ADVANTAGES: • Maximum efficiency of light collection in pixels on the micron scale • Ease and simplicity of manufacture; low cost • Reduces fabrication of lenses to a single step in the lithographic process • Works well across the chromatic spectrum • Greatly reduces cross-talk between adjacent pi
...

Co-registration of PET and Transrectal Ultrasounds (TRUS) Images for Improved Prostate Imaging and Biopsy

IB-2420 Dual PET-TRUS imaging co-registers positron emission tomography and transrectal ultrasound images taken during the same patient exam. The simulated “merged PET-TRUS image” (central image), helping to pinpoint any cancer present, will be used to guide diagnosis and potential treatments, as indicated at right. APPLICATIONS OF TECHNOLOGY: • Detects prostate cancer using accurately co-registered positron emission tomography (PET) and transrectal ultrasound (TRUS) imag
...

SATB1: a Fundamental Prognostic Marker and Therpeutic Target for Metastatic Breast Cancer

IB-2186 Published in Nature, March 2008. See link below. APPLICATIONS OF TECHNOLOGY: Screening for metastatic-prone breast cancers Using SATB1-specific inhibitors to treat breast cancer Designing assays to detect SATB1 expression in aggressive breast cancer cells Creating therapies for genes that express SATB1 ADVANTAGES: Provides a fundamental and powerful target for breast cancer therapy, since SATB1, once it is expressed in breast cancer, reprograms and orchestrates the e
...

Non-Transgenic Cell Lines for Identifying Target Premalignant Genes

IB-2023 Berkeley Lab's S3 cell lines display partial loss of differentiation in 3DirBM Cultures. Human breast epithelial cell lines were derived from a patient with fibrocystic breast disease. The epithelial tissue gave rise to non-tumorigenic S1 cells, from which more aggressive S2 cells were derived. Using colony size in three-dimensional laminin-rich basement membrane (3DlrBM) as an initial screening tool, the Bissell lab isolated three new cells lines, S3-A (mini), S3-B (midi), a
...

Method for Identifying New Breast Cancer Stem Cells and Targeted Therapies

JIB-2395 APPLICATIONS OF TECHNOLOGY: Development of new breast cancer therapies and diagnostic assays Researching cancer progression and remission ADVANTAGES: Specifically isolates human mammary stem and progenitor cell candidates from primary mammary tissue May identify new mammary stem cell markers Differentiates major cell types of the mammary gland from each other Advances stem cell research without the need for embryonic stem cells ABSTRACT: Mina Bissell of Berkeley La
...

Electrostatic-based DNA Microarray Technique

JIB-2397 Press Release, Berkeley Lab, June 30, 2008 The dream of personalized medicine — in which diagnostics, risk predictions and treatment decisions are based on a patient's genetic profile — may be on the verge of being expanded beyond the wealthiest of nations with state-of-the-art clinics. A team of researchers with the U.S. Department of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab) has invented a technique in which DNA or RNA assays — the key to genetic profi
...

Using Novel Enzymes to Produce Advanced Biofuels

EJIB-2540 APPLICATIONS OF TECHNOLOGY: Biosynthesis of carboxylic acids and lactones for biofuel production ADVANTAGES: Provides biofuel alternatives to ethanol The new enzymes can be modified to tailor-make a wide variety of lactones and carboxylic acids Controlled placement of ester linkages may result in enhanced combustion properties Esters and lactones are more hydrophobic than their corresponding alcohols, offering compatibility with the current fuel infrastructure Esters and l
...

Altering Bone Density by Modulating SOST Expression via Tissue-specific Enhancers

B-1944 APPLICATIONS OF TECHNOLOGY: Developing therapies for treating bone related conditions such as osteopenia, osteoporosis, fractures, and sclerosteosis Diagnosing and treating Van Buchem’s disease Conducting pharmacogenomics of current available osteoporosis treatments such as parathtroid hormone (PTH) Studying bone mineral density related diseases using transgenic and knockout animals ADVANTAGES: Enables the development of therapies for bone related diseases for which no ef
...

Synthesis of Colloidal Upconverting Monodisperse Nanocrystals for Biological Applications

Description of the technology: Synthesis, characterization, and spectroscopy of upconverting lanthanide-doped NaYF4 nanocrystals (NCs). Monodisperse cubic NaYF4 NCs were synthesized via a thermal decomposition reaction. Colloidally stable solutions of NaYF4 NCs in nonpolar organic solvents can be obtained. Benefits: - The synthetic method uses technical-grade solvents and ligands - Produces highly luminescent and uniform NCs; (27.6 nm ± 1.6). - The colloids of the lanthanide-doped NCs exhi
...

Silica Nanoboxes

Microporous crystalline materials (e.g. zeolites, aluminophosphates), also called molecular sieves, are widely used as catalysts, adsorbents, and membranes. Two factors largely determine the utility of such materials: their regular nanoscaled size of pores and cavities, and their structural rigidity and stability under quite harsh working conditions. However, the maximum pore size of these materials is about 0.8 nm. Extending the application of porous crystalline materials to encompass the contr
...

74.1, Vertebrate Cytoplasmic Dynein

This monoclonal antibody is for one of the polypeptides of vertebrate cytoplasmic dynein. This antibody is an IgG and is therefore more useful than IgM; it is also prepared with mammal (cow) antigen, not chicken. This clone is useful for western immunoblots, immunocytochemistry and immunoprecipitation. The MAb has affinity for eleven of the isoforms of the 74kD polypeptide of purified bovine brain cytoplasmic dynein resolved by two dimensional gel electrophoresis, and cross-reacts with cytoplasm
...

Method and Apparatus for Monitoring and Restoration of Shunt Function

General Information Hydrocephalus is a condition where cerebrospinal fluid (CSF) in the brain accumulates in the ventricles and increases pressure within the skull. It is routinely treated through insertion of a catheter in the brain connected to a shunt that acts to redirect CSF to other areas of the body to be reabsorbed, such as the abdomen or heart. Often however, the catheter becomes blocked with debris, either tissue from the insertional scar or others, resulting in shunt-failure. Curre
...

Liquid-phase Reactor for Catalytic Production of Biomarkers from Biological Materials

Liquid-phase Reactor for Catalytic Production of Biomarkers from Biological Materials - A method for rapid identification of biological materials such as Anthrax. General Information In 2001, just a week after the terrorist attacks on the World Trade Center in New York City, high quality anthrax spores (caused by the gram positive Bacillus anthracis bacteria) were sent to the offices of two U.S senators and several news media offices resulting in the sickening of seventeen Americans and the
...

Notify me of New Entries on this Page

You can receive an email whenever a new patent is added to this category. You can unsubscribe at any time.