Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes
Disclosed is a microarray of binary nucleic acid probes for the detection of one or a plurality of nucleic acid analytes in a complex sample in a single high throughput assay with extraordinary specificity under physiologic conditions Binary nucleic acid probes that generate a detectable signal when bound to analyte are suitable for use in the micro arrays, including binary deoxyribozyme or ribozyme probes, nonenzymatic binary probes for fluorescent detection, nonenzymatic binary dye-binding probes, and binary split enzyme peroxidase probes for visual detection of nucleic acids Nonenzymatic binary probes that bind to reporter oligonucleotides are also disclosed.
KOLPASHCHIKOV DMITRY [US]
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