Reduced Dye Probes for the Detection of Radical Oxygen Species
Reduced dyes, such as hydrocyanines, deuterocyanines, and/or other deuterated dyes, such as deuteraled leuco dyes, capable of detecting one or more reactive oxygen species are described herein The reduced dyes are generally prepared by reducing the oxidized dye with a reducing agent, such as sodium borohydpde or sodium borodeuteride The reduced dyes exhibit little or no fluorescence due to the disrupted 1t conjugation However, upon reaction with ROS, the reduced dyes are oxidized, regenerating the extended 1r conjugation and causing a substantial increase in fluorescence intensity In many case, the oxidized dye starting material is generally membrane impermeable However, upon reduction, many of the reduced dyes are membrane pemieable Thus, reduced dyes can accumulate in cells and/or tissue Once inside the cell or tissue, the reduced dye is reoxidized upon reaction with ROS, and the oxidized dye again becomes membrane impermeable, trapping the dye within the cell.
MURTHY NIREN [US]; TAYLOR W ROBERT [US]; KUNDU KOUSIK [US]; KNIGHT SARAH F [US]; LEE SUNGMUN [US]
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