High Affinity Recombinant Sea Lamprey Antibodies Selected By a Yeast Surface Display Platform
The present invention relates to a Yeast Surface Display (YSD) vector for expression of VLR proteins by yeast, wherein the vector includes nucleotide sequences encoding segments of yeast flocculation proteins FloIp, such as the leader and C-terminal segments, a homologous recombinant cassette and a geneticin/kanamycin resistance gene. The vector can be used for expression of VLR that may be effective in diagnostic applications (e.g., protein chip, immunohistochemistry, flow cytometry), immunoaffinity purification, and for engineering novel fusion proteins.
PANCER ZEEV [US]; MARIUZZA ROY A [US]; TASUMI SATOSHI [JP]; VELIKOVSKY CARLOS A [US]
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