Affinity Displacement Molecular Separation
Provided herein are methods for identifying molecules that compete with a ligand for binding to a protein. The method comprises contacting a ligand with a proteome to allow protein members of the proteome to bind with the ligand, followed by the removal of the unbound ligand. At least one test molecule is added to the mixture and after passing the mixture through an ultrafiltration membrane, the filtrate is assayed for the displaced ligand. The presence of the ligand within the filtrate indicates the molecule competes with the ligand for binding to at least one protein member within the proteome.
HAYSTEAD TIMOTHY A J [US]
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