LuSIV Cells: a Highly Sensitive Detection System for Infectious HIV and Related Retroviruses

JHU researchers constructed a reporter cell line, derived from human CEMx174 cells, that can be used to readily identify cells that are infected with HIV and related retroviruses. This cell line, designated LuSIV, contains a reporter construct comprising the firefly luciferase reporter gene under the control of a minimal SIV long terminal repeat (LTR) and infection is detected through a straightforward, quantitative luciferase assay. This cell line is highly sensitive to infection by a broad range of primary and laboratory strains of HIV/SIV and is capable of detecting a single round of HIV/SIV infection in 24 hours or less. Description (Set) Proposed Use (Set) This cell line provides a highly sensitive and quantitative detection system for infectious HIV and related retroviruses, and can be used for high throughput assays to screen for anti-virals and other inhibitors of infection

Inventor(s): Clements, Janice E.

Type of Offer: Licensing



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