Medical Patents for Sale or License

Detection of Signaling Molecules in a Biological Environment

This Patent focus on the detection of bacteria and other pathogens within the medical or agricultural areas. The detection uses a unique method of detecting in real-time, in situ, the Quorum Sensing signaling molecules rather than the bacteria itself.
[PURCHASE DETAILS HERE]


Sleep Apnea Oral Device

A fully adjustable oral device employing a palate lifter and tongue retainer to maintain an airway during sleep for those afflicted with obstructive sleep apnea and snoring.
[PURCHASE DETAILS HERE]


Methods for Prevention and/or Treatment of Capsular Contracture

Prevention and/or treatment of capsular contracture utilizing a repositioned topical ointment. The patent also encompasses the use of the product on fibrotic tissue with other etiologies as its formative basis.
[PURCHASE DETAILS HERE]


Detection of Signaling Molecules in a Biological Environment

This Patent focus on the detection of bacteria and other pathogens within the medical or agricultural areas. The detection uses a unique method of detecting in real-time, in situ (within the field or on a person), the Quorum Sensing signaling molecules rather than the bacteria itself. These signaling molecules are much smaller than the bacteria, diffuse much more readily within the environment and identify only live bacteria. This technology could also be useful for Quorum Quenching methods whe
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Methods for Prevention and/or Treatment of Capsular Contracture

J&E Solutions holds a US patent for the prevention and/or treatment of capsular contracture utilizing a repositioned topical ointment. The patent also encompasses the use of the product on fibrotic tissue with other etiologies as its formative basis. The topical composition is comprised of a pharmaceutically acceptable keratolytic, a pharmaceutically acceptable protein denaturant, a hydrating agent, and combinations thereof which are disclosed. Our firm, founded by a registered nurse and plas
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Genotypic tumor progression classifier and predictor

Actively dividing tumors appear to progress to a life threatening condition more rapidly than slowly dividing tumors. Assessing actively dividing tumors currently involves a manual assessment of the number of mitotic cells in a histological slide prepared from the tumor and assessed by a trained pathologist. Disclosed is a method for using cumulative information from a series of expressed genes to determine tumor prognosis. This cumulative information can be used to categorize tumor samples into
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Omni-directional Remote-controlled Mobility Apparatus

An omnidirectional mobility apparatus. The current invention is an omni-directional, remote-controlled mobility apparatus, similar to a wheelchair, which allows the transport of an individual or object(s). The device includes a cubic-shaped base with wheel wells on each vertical side of said base. Within each wheel well is a wheel set, such that the front and rear wheel sets face each other and the left and right wheel sets face each other. The circumference of each wheel set includes a pluralit
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Auto-regulation System for Intraocular Pressure

A device and system for controlling pressure via fluid exchange in the body is presented. The device and system may be used to control intraocular eye pressure. The system is comprised of four main components: a cannula, a sensor, a controller and a pump. The system works to engage in a fluid exchange which slowly increases/decreases intraocular pressure until a desired level is reached. Once the desired level is reached, the intraocular pressure is held steady and any external pressure perturba
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Osteotome System

A plurality of osteotomes that are linked together via a recess and a matching projection such that the osteotomes remain linked when used to manipulate bone.
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Endoscope System with Disposable Sheath

Listing Purpose We already have the product manufactured. Now we are seeking partners to license our product. Background Current conventional digestive endoscopy carries risk of deadly cross infections, and the reprocessing procedure is time consuming. To solve the problem, we developed an anti-infection digestive endoscopy device which comes with flexible endoscopes (gastroscope and colonoscope) and disposable sheath system so that all contaminated parts during endoscopy could be discarde
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Materials and Methods for Reduction of Protein tau and Treatment of Neurodegenerative Diseases

The subject invention provides a myricanol compound that is in predominant form of (+)-.alpha.R,11S-myricanol as compared to (-)-.alpha.S,11R-myricanol. In one embodiment, the (+)-.alpha.R,11S-myricanol is isolated from Myrica cerifera, and is in about 86% enantiomeric excess of (-)-.alpha.S,11R-myricanol. The subject invention also pertains to therapeutic compositions and methods for treatment of neurodegenerative diseases, in particular, neurodegenerative diseases associated with abnormal accu
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"Composition and Manufacturing Processes of a Toxicity Free Botanical Drug for Curative Treatment of Chronic Diseases"

This invention discloses composition and manufacturing processes of a toxicity free botanical drug formulation for curative treatment of chronic inflammatory diseases.
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Skin patch instrument for treating pain

A therapeutic patch for treating pain capable of compressing a tender area up to a boundary thereof and/or an entire induration with metallic grains or crushed stones by a treatment method in accordance with a classification determined after clarifying a site of pain by pressing a skin with a pressing bar or fingers (first to fourth fingers) to classify the cause of pain, thereby finding the presence of a geographical tender area and/or the presence of an induration on a skin as the cause of pai
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Medical Transcription System including Automated formatting means and associated method

I am looking to sell my ( Utility Patent) for Medical Transcription System including Automated Formatting Means and Associated Method ( For an upfront fee of $1 Million U.S. dollars plus a % of Royalties from all profits made.) This is a technology that automates Medical Transcription (of any form of Transcription so therefore can be used by teachers, lawyers, court reporters, business people, etc. ) It formats the report based on what type of report you are doing and makes the report in real ti
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Full Pot - the Pot That Does the Lot

Introducing the ‘Full-Pot’. The unique design of the ‘Full-Pot’ bottle permits the use of the entirety of the contents, ensuring no waste. “The pot that does the lot” has an array of purposes: ideal for nail polishes, medicine, perfumes and essential oils. As a result, the multifunctional ‘Full-Pot’ is compatible with a variety of fitments, including: brushes, pipettes, droppers and syringes. This is how it works: The bottle consists of two chambers: the lower chamber i
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Methods for resection of a luminal structure

Provided is a method of resecting a luminal structure, such as a colon. A first luminal structure and a second luminal structure are defined by severing the original luminal structure. The second luminal structure is secured to the distal end of an elongate body disposed within the second luminal structure. Refraction of the elongate body forms a circumferential fold in the second luminal structure and isolates a tissue mass between the circumferential fold and the distal end of the elongate bod
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Asymmetric Disc Distracting Cage

An inter-vertebral cage with a wedge shape eccentrically located on the leading end of the cage. The ectopically shaped leading edge of this embodiment facilitates easier interbody spacer placement with less risk of nerve injury, as well as with likely decreased risk of destruction of the end plate during the distraction process when the interbody cage is impacted.
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Extending Tissue Preservation

A method of sustaining cells is provided. The method can include providing a non-perfluorocarbon cell storage medium, providing a pre-oxygenated liquid perfluorocarbon in contact with the storage medium, and placing the cells in contact with the storage medium but not in contact with the perfluorocarbon. Additionally, the method can result in increased corneal cell viability compared to corneal cells placed in a non-perfluorocarbon cell storage medium without being in contact with a pre-oxygenat
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Human Immunosuppressive Protein

A composition of an immunosuppressant protein (HISP) which is achieved by the steps of obtaining supernatant from hNT neuronal cells; exposing the supernatant to preparative polyacrylamide gel; placing the active isoelectric fraction on a Blue Sepharose column to bind albumin; and collecting the free fraction containing the concentrated, isolated HISP. The HISP is anionic, has a molecular weight of 40-100 kDa, an isoelectric point of about 4.8 and is obtained from the supernatant of hNT cells. H
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Salmonella-based anti-cancer vaccine

We offer a technology of vaccine-based cancer therapy developed at the Jagiellonian University (Krakow, Poland). The immunotherapy is based on genetically modified bacterial strain of Salmonella VNP20009 that is able to target and preferentially colonise tumour tissue. Tumour targeting of VNP20009 was enhanced by single chain antibody fragments specific to carcinoembryonic antigen, which are expressed on the surface of the bacteria. Further genetic modifications were introduced to the strain to
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Device for supporting a person in a sitting position.

Description of my product(what it is in non-technical terms):LAKLIFT(tm)is a device to relieve pain caused by spinal pressure while sitting. It works when nothing else would seem to be effective except surgery. LAKLIFT(tm) relieves back pain by reducing weight and pressure on the spine naturally.
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Topical Anesthetic for Expedited Wound Healing and Pain Relief

Heals cuts, burns, acute wounds, acute diabetic wounds, herpatic lesions, shingles rash, insect bites and stings, stomatitis, and radiation burns. Most lesions heal in 24 to 48 hours without infection or reported pain. University studied with pre-clinical and clinical trials. Topical has been published and presented to major national organizational synopsis conventions. Stable for over 24 hours and grows no bacteria. Over 60,000 patients through prescription have received the topical with n
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Quick-E Emergency ECG Electrode Chest Pad

A disposable ECG electrode chest pad that has pre-wired electrodes in the proper positions which can be applied in one step and easily connected to nearly any ECG machine with pre-existing adapters. It can be used in any setting an ECG would be used, in addition to being used to monitor patients in the hospital setting by easily removing the unnecessary V leads. It can also be adapted with defibrillator pads for emergency situations.
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Device for Giving Liquid Medicine to Children

We own one US Patent #7654977 that we are interested in selling/auctioning. The protected device product has been developed and sold in Europe for delivering liquid medicines to infants and toddlers under the mark “MEDICINUS”. Please see some pictures below.
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Real Time Dose Verification in Radiotherapy

A team from the Universities of Bristol and Swansea together with University Hospitals Bristol NHS Foundation Trust has developed a novel radiation detector device for determining the dose from Intensity Modulated Radiotherapy (IMRT) in real time. The detector is positioned upstream of the patient (between radiation source and patient) and is a very thin silicon detector camera system.
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“Deep” needle holder

Introduction: A number of open urological procedures demand deep and narrow surgical fields. This fact in correlation with special circumstances (like hemorrhage) makes many times the manipulation of a curved needle with a conventional needle holder very difficult, even for an experienced surgeon. This invention is designed in purpose to increase the flexibility and safety in grasping and suturing with a curved needle in difficult circumstances, where the conventional needle holders are ins
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Computer peripherals sterilization system

A KEYBOARD ADD-ON THAT PROVIDES A RULES-BASED, CONTROLLED PROCESS OF ELIMINATING BACTERIA AND GERMS FROM ANY EXISTING KEYBOARD, MOUSE, CELLPHONE, IPAD OR SIMILAR DEVICES IN 10 SECONDS OR LESS. EXISTING KEYBOARD CAN BE DISABLED TO GUARANTEE THE NEXT USER A FULLY STERILE ENVIRONMENT. FULLY USB CONTROLLED, IN IDLE MODE THE ADD-ON PROVIDES A UNIQUE ORGANIZER / USB HUB FUNCTIONALITY. VARIOUS MODES AS WELL AS FULL USAGE REPORTS ARE AVAILABLE FOR TRACKING. IDEAL FOR PUBLIC USE OR MEDICAL FACILITIES
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Special Bed for prevention of Bedsores

It is a BED for the totally INVALID person prone to BED SORES. The paraphrased abstract reads as follows: A specialized bed will prevent bedsores, heal existing bedsores, with no repositioning of person required. The bed is provided with a series of BANDS that can be made to be TAUT and horizontal, or relaxed to be LIMP and shaped like a downward arc. The person is supported on the bands that are taut. Alternate bands are taut and the others are limp. After a brief period, the the bands
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Lined Balloon Mounted Stent

A stent that is implanted by catheter to narrow or occlude the lumen of any vessel in a graded reversible pattern as needed. May be used to perform patent ductus arteriosus closure in neonates, pulmonary artery banding, reverisble control or occlusion of bleeding aneurysms..etc in all age groups.
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Serotonin Transporter Gene and Treatment of Alcoholism

The gene responsible for encoding SERT has a functional polymorphism at the 5'-regulatory promoter region, which results in two forms, long (L) and short (S). The LL-genotype is hypothesized to play a key role in the early onset of alcohol use. The present invention discloses the differences in treatment and diagnosis based on the L or short genotypes as well as on a single nucleotide polymorphism of the SERT gene, the 3' UTR SNP rs 1042173. The present invention demonstrates the efficacy of usi
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Use of S-Nitrosothiol Signaling to Treat Disordered Control of Breathing

The present invention is directed to a method of treating disordered control of breathing including the treatment of apnea and hypoventilation associated with congenital or acquired brain stem abnormalities. Specifically the invention is directed to treating disordered control of breathing by administering an S-nitrosylating agent selected from the group consisting of ethylnitrite, glutathione, nitric oxide, S-nitrosocysteine, S-nitrosoglutathione, S-nitroso-L-cysteinyl glycine, N-acetyl cystein
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Combination of HGF Inhibitor and PTEN Agonist to Treat Cancer

The present invention is directed toward a method of treating cancer by administering to a patient an inhibitor of Hepatocyte Growth Factor and an agonist of PTEN.
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Method and System for Fusing a Spinal Region

A method and system for fusing a region in the spine involve the use of at least one guide tube to pass instruments and substances into the spinal region in a minimally invasive manner. In the preferred practice of the method, a guide tube is anchored to a vertebra and the guide tube is moved to thereby position the vertebra. A steerable drilling tool is passed through the guide tube and steered into position to abrade at least a portion of an intervertebral disc and thereby create a cavity in t
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Compositions and Methods for Treatment of Leukemia

The invention relates generally to effective treatment leukemia. In particular, the present invention provides compositions and methods to inhibit the interaction of menin with MLL and MLL-fusion oncoproteins, and well as systems and methods to screen for such compositions.
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Use of pramipexole to treat amyotrophic lateral sclerosis

The present invention is directed to compositions comprising pramipexole and the use of such compositions treat neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). As shown in Fig. 6B the mean + 1 -SEM serum 2,3-DHBA levels for the 12 ALS participants decreased significantly after pramipexole treatment.
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Inhibiting CAV3 isoforms and the delta25B splice varients for the diagnosis and treatment of cancer

The present application is directed to an antibody that specifically binds to a Cav3 isoform or its '25 splicing variants thereof.
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L- OddC Prodrugs for Cancer

The main drawback in the use of most nucleoside anticancer agents originates from their hydrophilic nature, of which property requires a high and frequent dosage for an intravenous administration. Unlike other nucleoside anti-tumor agents, troxacitabine appears to predominantly enter tumor cells by passive diffusion rather then by using nucleoside transporters, although this may be model dependent. Accordingly, in the present work, a small library of twenty troxacitabine prodrugs has been synthe
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Hybridoma Cell Lines and Monoclonal Antibodies Recognizing Anthrose Saccharide of Bacillus Anthracis

The present invention includes hybridoma cell lines and monoclonal antibodies that bind to the anthrose tetrasaccharide of Bacillus anthracis and methods using said antibodies.
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Antimicrobial Compositions and Methods of Use

Disclosed herein are antimicrobial compositions, kits, and articles of manufacture. Further disclosed herein are methods for treating surfaces, including tissue, inter alia, wounds, with the disclosed compositions.
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No-modified hemoglobins and uses therefor

Nitrosylhemoglobin can be produced by introducing gaseous NO into an aqueous solution of hemoglobin. It has been demonstrated that nitrosylhemoglobin in aqueous solution can be converted to SNO-hemoglobin upon introduction of oxygen to the solution, as is postulated to occur in the lungs. Nitrosylhemoglobin can be used in methods to produce the physiological effects of NO, for example, to reduce vasoconstriction and to inhibit platelet aggregation.
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Dual Specific Immunotoxin for Brain Tumor Therapy

We tested the in vitro and in vivo efficacy of a recombinant bispecific immunotoxin that recognizes both EGFRwt and tumor-specific EGFRvIII receptors. A single chain antibody was cloned from a hybridoma and fused to toxin, carrying a C-terminal peptide which increases retention within cells. The binding affinity and specificity of the recombinant bispecific immunotoxin for the EGFRwt and the EGFRvIII proteins was measured. In vitro cytotoxicity was measured. In vivo activity of the recombinant b
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Anti-Tumor Antibodies

Chondroition sulfate proteoglycans represent excellent targets for anti-tumor immunotherapy. Antibodies which target such proteoglycans can be used alone, in combinations, armed with a cytotoxic payload or unarmed. Combinations of such antibodies can target different epitopes of the proteoglycans. Internalization of the antibodies can increase the toxicity of the payloads. Single chain variable regions are especially advantageous for such anti-tumor immunotherapy.
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Vaccine Vectors

The present invention relates, in general, to recombinant viral constructs expressing a protective antigen and to vaccine compositions comprising same. The invention further relates to a method of inducing an immune response, for example, to human immunodeficiency virus (HIV).
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Inhibiting Smad signaling promotes neuron regeneration

Regeneration of a lesioned CNS axon of a mature neuron, determined to be subject to regeneration inhibition by Smad2/3 signaling, is promoted by contacting the neuron with an inhibitor of Smad2/3 signaling sufficient to promote regeneration of the axon.
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Method for Augmenting the Effects of Serotonin Reuptake Inhibitors

A method of treating a subject for a serotonergic neurotransmission dysregulation disorder, comprises administering the subject a serotonin enhancer (e.g., a serotonin reuptake inhibitor) in an amount effective to treat the disorder; and concurrently administering the subject 5-hydroxytryptophan in an amount effective to enhance the activity of the serotonin enhancer, (e.g., serotonin reuptake inhibitor). In preferred embodiments the disorder is depression, anxiety, or substance abuse.
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Compositions and Methods for the Treatment of Seborrhea

Methods and compositions are provided for treating dermatological diseases, disorders, and pathologies, including seborrheic dermatitis, in a subject in need thereof. In some embodiments, methods are provided for the treatment of a dermatological disease, disorder, or pathology comprising, topically administering to a subject in need of dermatological treatment a composition comprising a therapeutically effective amount of bupropion, lithium or lithium salicylate, or combinations thereof.
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Compositions for Tissue Repair/regeneration

The invention provides compositions and methods for cardiac repair and/or regeneration of tissues such as myocardium.
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Anti-CD19 Antibody Therapy for Autoimmune Disease

The invention relates to immunotherapeutic compositions and methods for the treatment of autoimmune diseases and disorders in human subjects using therapeutic antibodies that bind to the human CD19 antigen and that preferably mediate human ADCC. The present invention relates to pharmaceutical compositions comprising human or humanized anti-CD19 antibodies of the IgG1 or IgG3 human isotype. The present invention relates to pharmaceutical compositions comprising human or humanized anti-CD19 antibo
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Chronic Lymphocytic Leukemia Prognosis and Treatment

Provided herein are methods for identifying a subject afflicted with chronic lymphocytic leukemia who is responsive to treatment with a chemotherapeutic agent by detecting the presence or absence of at least one APOE4 allele in the subject, the presence of an APOE4 allele identifying the subject as responsive to the treatment. Also provided are methods of treating a subject afflicted with chronic lymphocytic leukemia, including administering an estrogenic agent, an androgen withdrawal agent, an
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Anti-CD19 Antibody Therapy for Transplantation

The invention relates to immunotherapeutic compositions and methods for the treatment and prevention of GVHD, humoral rejection, and post-transplantation lymphoproliferative disorder in human subjects using therapeutic antibodies that bind to the human CD19 antigen and that preferably mediate human ADCC. The present invention relates to pharmaceutical compositions comprising human or humanized anti-CD19 antibodies of the IgG1 or IgG3 human isotype. The present invention relates to pharmaceutical
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Compositions and Methods for Treating Hair Loss Using Non-naturally Occurring Prostaglandins

A method for treating hair loss in mammals uses compositions containing prostaglandin F analogs. The compositions can be applied topically to the skin. The compositions can arrest hair loss, reverse hair loss, and promote hair growth.
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Methods and Compositions to Identify Increased Risk of Breast Cancer by Detection of CPG Island Methylator Phenotype (CIMP)

The present invention is directed to kits and methods for identifying a female subject as having an increased risk of developing breast cancer, comprising detecting a CPG island methylator phenotype (CIMP) in nucleic acid of the subject.
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Cancer Therapy

PROBLEM TO BE SOLVED: To provide cancer therapy. ; SOLUTION: A method for preventing or treating cancer is provided. In the method, low molecular weight antioxidants [e.g., mimetics of superoxide dismutase (SOD)] are generally used in cancer therapy, especially as an active agent or as a chemo- and/or radio-protectant. Compounds and suitable compositions for such a method are also provided. In addition, agents including methine (i.e. meso) substituted porphyrin and tetrapyrrole and suitable for
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Reagent for Autoimmune Disease and Treatment

PROBLEM TO BE SOLVED: To provide a reagent for the treatment of autoimmune diseases and a method. ; SOLUTION: This invention relates to a treatment method using an anti-CD22 monoclonal antibody having specific physiological characteristics. Especially, this invention relates to a method of treating B cell malignancies and autoimmune diseases by the administration of blocking anti-CD22 monoclonal antibody which is bound specifically to the first two Ig-like domains or an epitope in the first two
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Xanthine Derivatives for the Treatment of Vascular Depression

Methods and compositions are provided for treating vascular depression. The methods involve administering to a subject in need thereof a xanthine derivative in a therapeutically effective amount to treat vascular depression, particularly the xanthine derivatives pentoxifylline or propentofylline. The methods may further include administration of an additional therapeutic agent in combination with the xanthine derivative selected from the group consisting of a selective serotonin reuptake inhibit
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Methods of Subclassification of Ductal Carcinoma of the Breast

Provided herein are methods of determining the aggressiveness or indolence of a ductal carcinoma in situ lesion. Also provided are methods of developing treatment plans for subjects with a ductal carcinoma in situ lesion based on the aggressiveness of the lesion.
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Prevention of Flap Necrosis in Plastic Surgery

PROBLEM TO BE SOLVED: To provide a method for resolving or preventing vasoconstriction and preventing depletion of blood flow or restoring blood flow in a pedicle flap or any other microsurgery. ; SOLUTION: The method for preventing necrosis in a pedicle flap or in any microvascular surgery comprises topically applying to pedicle or other source of blood supply, a therapeutically effective amount of a vasodilator composition containing an NO or NO donor or a prodrug that causes formation of nitr
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Peripheral Reticular Pigmentary Change and Age-Related Macular Degeneration

Examination of 956 age-related macular degeneration cases showed that the complement factor H variant (Y402H, C allele at rs1061170) increases risk for the development of peripheral reticular pigmentary change. AMD phenotypes of 796 carriers of the CFH Y402H variant were compared to the AMD phenotypes of 160 non-carriers. Of 34 phenotypic features analyzed, only peripheral reticular pigmentary change (PRPC) was associated with this CFH variant (P-value 0.0006). The proportion of AMD cases with P
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Implants with membrane diffusion controlled active ingredient release

Implant having a structure for implantation in a human or animal body, comprises implant base body, primer layer applied at least partially to the surface of the implant, active ingredient layer consisting of one or more active ingredients applied to at least a portion of the surface of the primer layer and diffusion-controlling layer applied to at least a portion of the active ingredient layer, where the diffusion of the active ingredients of the active ingredient layer is controlled. An indepe
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Stabilization of Cells By Ionic Liquids

The present invention relates to the use of ionic liquids and/or compatible solutes for storing and stabilizing eukaryotic cells, above all blood cells such as leukocytes, platelets, or erythrocytes. The invention further relates to compositions which comprise eukaryotic cells and ionic liquids and/or compatible solutes, to medical products having ionic liquids and/or compatible solutes, and to a method for storing eukaryotic cells. The cells can be contained in cell structures (cell mixtures, t
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Implant and Bone Screw Having Interlocking Cams

The invention relates to a fixation device for bones, consisting of an implant (1; 11), having at least one through hole (2; 12), to be fastened to the bone, and of at least one bone screw (3; 13) to be fastened in said through hole (2; 12), fastening elements (5; 15) being arranged in the through hole (2; 12) and at the top end of the bone screw (3; 13) and engaging with each other. In order to allow various angular positions of the bone screw (3; 13) relative to the implant (1; 11) without col
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Use of Plant Extracts Comprising Polyphenol for Treating Adiposity and Diabetes Mellitus Type 2

The present invention relates to the use of plant extracts comprising polyphenol for treating adiposity and diabetes mellitus type 2. The extracts comprise specific polyphenol compounds, in particular procyanidin A3 isomers. The invention further relates to methods for producing extracts of said kind from plants, in particular from leaves and stems. The plants are preferably Ericaceae, for example vaccinium species, in particular lingonberry (Vaccinium vitisidea), blueberry (Vaccinium myrtillus)
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Bioequivalence Dialysis

The present invention relates to a method for treating blood disorders and blood disorders compensation. This includes steps in which one blood or blood plasma of a patient outside the body brings with a second liquid (bioequivalent) in contact with the second liquid blood or blood is modified, which includes at least granulocytes, platelets and red blood cells.The invention further relates to devices for implementing this method and the use of such a device or modified by blood or blood that gr
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Combination of an antibody and a bacterium, which expresses Fc-receptors or proteins, which bind the light chain of an antibody, useful for the treatment of tumors and their precursors, preferably col

Combination of at least one antibody and a bacterium, which expresses Fc-receptors or proteins, which bind the light chain of an antibody, is claimed, where at least one antibody is bound via its Fc-region to an Fc-receptor of the bacterium or via its light chain to protein of the bacterium, which binds the light chain of an antibody, where in more antibodies the light chains are same or different. - ACTIVITY : Cytostatic. - MECHANISM OF ACTION : None given.
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Bioequivalence Dialysis

The present invention relates to a method for treating blood composition disorders and blood function disorders. Said method comprises steps, in which blood or blood plasma of a patient is brought into extracorporeal contact with a second fluid (bioequivalent), wherein said second fluid is blood or modified blood, which comprises at least granulocytes, thrombocytes, and erythrocytes. Furthermore, the invention relates to devices for performing said method and to the use of such a device or blood
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Use of Opioids Or Opioid Mimetics for the Treatment of Resistant Cancer Patients

The use of opioids or opioid mimetics is suggested for the manufacture of a medicament for the treatment of resistant cancer patients.
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Inhibiting GSNOR

Treatment of myocardial infarction to reduce infarct size and organogenesis is provided by administration of agents selected from the group consisting of:
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Method of Providing Neuroprotection Using Substituted Porphyrins

Described herein are methods of treating ischemic injury comprising administering to a subject in need thereof a therapeutically effective amount of a substituted porphyrin compound. Also disclosed are methods of providing neuroprotection, methods of treating subarachnoid hemorrhage, methods of treating traumatic brain injury and methods of treating spinal cord injury using substituted porphyrins.
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3,28-disubstituted Betulinic Acid Derivatives As Anti-HIV Agents

Compounds according to Formula (I) are described along with compositions containing the same and methods of use thereof for the treatment of viral infections.
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Methods of Treating Lung Disease and Other Inflammatory Diseases

A method of treating an inflammatory disease in a subject in need thereof is carried out by administering the subject a gastrin-releasing peptide (GRP) inhibitor in a treatment effective amount. Suitable GRP inhibitors include compounds of the general formula (I) and pharmaceutically acceptable salt or prodrugs thereof.
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Predicting Coronary Artery Disease and Risk of Cardiovascular Events

Methods of assessing the risk of cardiovascular disease in a subject by detecting the level of at least one metabolite in a sample from the subject are disclosed herein. The level of the metabolite is indicative of the risk of cardiovascular disease in the subject. The metabolites may be acylcarnitines, amino acids, ketones, free fatty acids or hydroxybutyrate. The cardiovascular disease may be risk of a cardiovascular event, presence of coronary artery disease or risk of development of coronary
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Prevention of Receptor Desensitization

PROBLEM TO BE SOLVED: To provide a method for treatment of diseases relating to G protein receptor kinase activity or patients under pathological conditions of the same. ; SOLUTION: The desensitization of a receptor controlling the diseases can be prevented by inhibition of the G protein receptor kinase. This is applicable to patients with, e.g., cardiac failure, attached with a left ventricle cardiac device or a cardiac pump after surgical operation or those immediately before the surgical oper
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Methods and Compositions for Regulating HDAC6 Activity

The present invention provides methods and compositions for inhibiting Hsp90 activity in a cell, comprising contacting the cell with an inhibitor of histone deacetylase 6 (HDAC6)
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Treatment of glycogen storage disease type II

Methods of treating glycogen storage disease type II, by administering acid alpha -glucosidase, are described, as are compositions for use in treatment of glycogen storage disease type II.
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Compositions and Methods for Modulating S-nitrosogluthione Reductase

Disclosed herein are methods and compositions for modulating the levels and/or activity of S-nitrosoglutathione reductase (GSNOR) in vivo or in vitro. Specifically disclosed are GSNOR deletion constructs, host cells and non-human mammals comprising GSNOR deletions, and methods of screening employing GSNOR deletion mutants. Also specifically disclosed are reagents and procedures for measuring, monitoring, or altering GSNOR levels or activity (as well as nitric oxide and S-nitrosothiol levels) in
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Use of Hasf As a Protective Agent Against Ischemic Tissue Damage

A method of inducing a protective response against ischemic tissue damage is carried out by administering to subject a composition comprising an HASF polypeptide. The composition is administered prior to an ischemic event such as myocardial infarction to reduce tissue damage associated with such an event.
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Neuroactive Steroid Compositions and Methods of Use Therefor

Provided are methods for ameliorating a symptom of a neuropsychiatric disorder in a subject. Also provided are methods for ameliorating at least one physical symptom or at least one psychological symptom resulting from tobacco cessation in a subject, methods for ameliorating a symptom of Alzheimer's disease or other cognitive disorder in a subject, methods for ameliorating a symptom of schizophrenia, schizoaffective disorder, or other psychotic disorder in a subject, methods for ameliorating a s
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Compositions and Methods for Promoting Nasal Patency and Treating Neurogenic Bladder Using Prostaglandins

The invention provides methods for maintaining or promoting patency (e.g., nasal patency) comprising administering to a subject in need thereof an effective amount of at least one prostaglandin F analog (PGF) as described herein, methods for relieving nasal congestion comprising administering to a subject in need thereof an effective amount of at least one PGF as described herein, methods of treating neurogenic bladder comprising administering to a subject in need thereof an effective amount of
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Forming Iron Nitrosyl Hemoglobin

Contrary to current opinion that nitrite in general oxidizes hemoglobin with elimination of active nitric oxide, deoxygenated hemoglobin reacts with low concentration inorganic nitrite to produce very stable iron nitrosyl hemoglobin which on delivery into the body is converted to a hemoglobin capable of nitric oxide delivery and provides vasodilator and antiplatelet activity. This provides basis for ameliorated risk blood product transfusions.
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Method of Treating Glycogen Storage Disease

The present invention relates, in general, to Glycogen Storage Disease and, in particular, to a method of treating Glycogen Storage Disease-type-III and to compounds and compositions suitable for use in such a method.
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Improved Device and Method for Delivery of a Medicament

The disclosure relates to an improved method of enhancing nicotine concentrations in a gaseous carrier. The methods are adaptable to the delivery of nicotine for therapeutic effect in various diseases, in particular nicotine for tobacco product use cessation, substitution and/or harm reduction. The disclosure further relates various devices and device design principles for practicing these methods.
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Treatment of Wnt/frizzled-related Diseases

Methods of treating Wnt/Frizzled-related diseases, comprising administering niclosamide compounds are provided. Methods of predicting whether a disease will respond to treatment with a niclosamide compound are also provided.
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Smoothened Receptor Modulators

The present invention relates, in general, to the Smoothened receptor and, in particular, to a method of modulating Smoothened receptor activity and to compounds and compositions suitable for use in such a method.
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Compositions and Methods for Inhibiting Hair Growth

A method for inhibiting hair growth in mammals using compositions containing FP receptor antagonists (e.g., prostaglandin F analogs that are block activation of the FP receptor). The compositions can be applied topically to the skin and/or hair. The compositions can arrest hirsutism or hypertrichosis, reverse hirsutism and hypertrichosis, and further prevent hair growth. These compositions can also be used to protect hair from chemical or radiation-induced alopecia or hair loss. These compositio
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Use of an Agent That Restores Tissue Perfusion and Oxygenation

The presently disclosed subject matter provides methods for increasing perfusion in hypoxic regions of tissues in subjects. Also provided are methods for treating diseases and/or disorders associated with hypoxia in subjects, methods for increasing sensitivity of tumors to radiation and/or chemotherapy treatments, methods for delaying tumor growth in subjects, and methods for inhibiting tumor blood vessel growth in subjects. In some embodiments, the presently disclosed methods involve administer
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Inhibition of HIF-1 activation for anti-tumor and anti-inflammatory responses

The presently disclosed subject matter generally relates to methods and compositions for inhibiting the expression and/or activation of hypoxia-inducible factor 1 (HIF-1) genes in a cancer cell, tissue or tumor. More particularly, the methods disclosed herein relate to inhibition of HIF-1 activation in a tumor, increasing sensitivity of a tumor cell to radiation and/or chemotherapy, delaying tumor growth, inhibiting tumor blood vessel growth, inhibiting inflammatory responses in a cell through t
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Compositions and Methods for the Treatment of Radioresistant Glioma Stem Cells

Compositions and uses of gamma secretase inhibitors (GSIs) for the treatment and inhibition of gliomas in subjects in need thereof are provided. Methods for administering gamma secretase inhibitors in association with radiation for the treatment of radiation resistant gliomas are described. Compositions for the inhibition of Notch signaling and activity are provided.
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Stem Cell Derived Factors for Treating Pathologic Conditions

A purified paracrine factor of a mesenchymal stem cell, such as a Secreted frizzled related protein (Sfrp) is useful to reduce cell death an/or tissue injury associated with ischemic condtions.
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Substituted porphyrins and their therapeutic use

The present invention relates, in general, to a method of modulating physiological and pathological processes and, in particular, to a method of modulating cellular levels of oxidants and thereby processes in which such oxidants are a participant. The invention also relates to compounds and compositions suitable for use in such methods.
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Novel Medical Uses for No and No Donor Compounds

A body part is preserved using nitric oxide and/or a nitric oxide donor that does not directly release nitric oxide or a red blood cell nitrosylating agent, preferably ethyl nitrite to facilitate oxygen supply. A subject at risk for developing high altitude illness is administered a red blood nitrosylating agent in gaseous form that does not directly release nitric oxide, preferably ethyl nitrite.
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Inhibition of Inter-alpha Trypsin Inhibitor for the Treatment of Airway Disease

It is disclosed herein that blockade of inter-alpha-trypsin inhibitor (IaI) prevents the development of airway hyperresponsiveness in animal models of asthma and chronic obstructive pulmonary disease. Provided herein are methods of treating an airway disease or disorder in a subject by administering to the subject a therapeutically effective amount of an inhibitor of IaI. Also provided is a method of preventing or reducing airway hyperresponsiveness in a subject by administering to the subject a
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Methods and Compositions for Treating Urinary Tract Infections Using Agents That Mimic Or Elevate Cyclic Amp

Methods and compositions are provided for treating a urinary tract infection (UTI). The methods involve administering to a subject in need thereof a cAMP elevator or agent that mimics cAMP, particularly a labdane diterpene such as forskolin or a derivative or analog thereof in a therapeutically effective amount to treat a UTI. The methods may further include administration of at least one cAMP elevator in combination with one or more additional active compounds from other classes of therapeutic
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Systems and Methods for Determining Hemoglobin Concentration Utilizing Diffuse Reflectance At Isosbestic Wavelengths

Systems and methods for determining hemoglobin concentration utilizing diffuse reflectance at isosbestic wavelengths are provided. In one embodiment, the method includes emitting light onto a tissue mass, measuring diffuse reflectance from the tissue mass, and calculating a ratio of diffuse reflectance intensities at two isosbestic wavelengths using the measured diffuse reflectance. The method also includes determining a hemoglobin concentration associated with the tissue mass by applying the ca
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Inhibiting GS-FDH to Modulate No Bioactivity

Patients needing NO donor therapy or inhibition of pathologically proliferating cells or increased NO bioactivity are treated with a therapeutically effective amount of an inhibitor of glutathione-dependent formaldehyde dehydrogenase.
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Anti-lipid Antibodies

The present invention relates, in general, to anti-lipid antibodies and, in particular, to methods of inhibiting HIV-I infection using anti-lipid (e.g. anti-phospholipid) antibodies.
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A Method of Detecting Antibodies and Antibody-hiv Virion Complexes

The present invention relates, in general, to HIV and, in particular to a method of detecting anti-HIV antibodies and antibody-HIV virion complexes.
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Treatment for Diseases Relying on Discovery That Thioredoxin Mediates Nitric Oxide

Patients having a disease associated with high level of thioredoxin system activity or a requirement for nitric oxide, e.g. large cell lymphoma or restenosis, are treated with a thioredoxin reductase inhibitor, e.g. auranofin or arsenic trioxide, and a nitric oxide donating compound, e.g. isosorbide mononitrite or isosorbide dinitrite or nitroglycerin or S-nittrosothiol. Patients having a disease associated with nitric oxide synthase overexpression or increased activity, e.g. Parkinson's disease
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Reversible Platelet Inhibition

The present invention relates, in general, to receptors and to platelet aggregation and, in particular, to a method of inhibiting platelet aggregation using an aptamer that binds to and inhibits the activity of a receptor, such as glycoprotein IIb/IIIa (gpIIb/IIIa), and to aptamers suitable for use in such a method. The invention also relates to antidotes to antiplatelet agents and to methods of using such antidotes to reverse aptamer-induced platelet inhibition. The invention further relates to
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Therapeutic Agents for Neurodegenerative Diseases

Methods of treating diseases involving protein aggregation are provided. Methods of identifying and selecting an autophagosome/lysosome fusion enhancer are also provided.
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Methods of Treating Disease-induced Ataxia and Non-ataxic Imbalance

Methods for treatment of disease-induced ataxia and non-ataxic imbalance are disclosed. The methods involve treating a patient with a compound having nicotinic acetylcholine receptor activity.
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Method of Treating Chronic Myelogenous Leukemia Cells

Here, the inventors disclose the treatment of imatinib mesylate resistant chronic myelogenous leukemia cells with a cotreatment of vorinostat (SAHA, suberoylanilide hydroxamic acid) and dasatinib, a dual Abl/Src kinase (TK) inhibitor. Combined treatment of cultured human CML and BaF3 cells with vorinostat and dasatinib induced more apoptosis than either agent alone, as well as synergistically induced loss of clonogenic survival, which was associated with greater depletion of Bcr-Abl, p-CrkL and
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Delta-tocotrienol Treatment and Prevention of Pancreatic Cancer

Methods are disclosed for treating neoplastic disorders, such as pancreatic cancer, using tocotrienols; namely, gamma-tocotrienol and delta tocotrienol. The antitumorogenic effects of these compounds are shown both in vitro and in vivo using several human pancreatic cancer cell lines and MIA-PAC A2 human pancreatic cancer cells xenografted in nude mice. Also disclosed are methods of testing the efficacy of potential chemotherapeutic agents by measuring their effect on surrogate endpoint biomarke
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Materials and Methods for Treating Allergic and Inflammatory Conditions

The subject invention provides for the utilization of bone-marrow derived stem cells in the treatment of allergic and inflammatory diseases. In one embodiment, the invention provides for treatment of asthma. Bone-marrow derived stem cells can be used for decreasing inflammation and alter the course of immune response in the lung.
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Urodilatin Cancer Treatment

Urodilatin, a peptide formed in the kidney, has significant anticancer effects eliminating two-thirds of cancer cells within 24 hours.
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Bone marrow-derived neuronal cells

Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC administered into the ventricle did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most orderly integration of BMSC was in the laminar distribution of cerebellar Purkinje cells, where the BMSC-derived cells took on the Purkinje
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Prevention, Treatment, and Diagnosis of Alzheimer's Disease Through Electromagnetic Field Exposure

According to a first embodiment the invention includes a method of treating a neurological disorder, such as Alzheimer's Disease, in a subject in need thereof by positioning an electromagnetic field emitting source proximal to the subject and exposing the subject to an electromagnetic field having a predetermined frequency for a predetermined absorption period. Preferably, each individual treatment (comprising exposure to the predetermined frequency for the predetermined absorption period) is co
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Compositions and Methods for Enhancing Neuroprotection Via Administration of Stem Cells and Blood Brain Barrier Permeabilizers

The present invention provides compositions and methods for enhancing the neuroprotective effect of umbilical cord blood cells. More particularly, the present invention provides methods of treating neurodegenerative disorders by administering umbilical cord blood cells and a substance capable of permeabilizing the blood brain barrier. In one embodiment, the blood brain barrier permeabilizer is mannitol. In another embodiment, the blood brain barrier permeabilizer is Cereport.
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Hucbc Treatment of Amyloid Associated Disease

Administration of human umbilical cord blood cells (HUCBC) or HUCBC-derived plasma is used to treat amyloid-based diseases, such as Alzheimer's disease, Huntington's disease, cerebral amyloid antigopathy, and type-II diabetes. Modulating inflammatory reactions by infusing HUCBC resulted in a marked reduction of amyloid plaques and immune-associated cellular damage. HUCBC infusion also significantly reduced cerebral amyloid angiopathy in mice models. These effects were associated with suppression
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Reelin Rescues Cognitive Function

Disclosed are methods of influencing, and enhancing, cognitive function by increasing, and/or preventing interference with, Reelin levels as well as Reelin signaling. Cognitive function is improved, in a subject in need thereof, by administering a therapeutically effective amount of Reelin, a Reelin-specific modulator or an agonist of a lipoprotein receptor to the subject. The lipoprotein receptor can be selected from candidates such as ApoER2 and VLDLR. As disclosed herein, agonists of the lipo
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Inhibitors of Akt/PKB with Anti-tumor Activity.

The subject invention concerns materials and methods for inhibiting the Akt/PKB pathway. In one embodiment, a compound of the invention inhibits kinase activity and/or phosphorylation levels of Akt proteins. The subject invention also concerns methods for inhibiting or killing a cancer cell or other cell in which expression of an Akt protein is elevated or constitutively active, comprising contacting the cell with an effective amount of a compound of formula I. The subject invention also concern
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Control of Nk Cell Function and Survival By Modulation of Ship Activity

Inhibition of dendritic cell function in solid organ grafts or allogeneic bone marrow transplants prior to or during engraftment by blocking SH2-containing inositol phosphatase (SHIP) expression or function is taught as a method of abrogating immune rejection and thereby increasing the efficacy of engraftment of an allogeneic bone marrow transplant or solid organ allograft or xenograft. Also disclosed is a transgenic mouse having the genotype SHIP-/- which exhibits enhanced survival following mi
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Methods of Diagnosing and Treating Cancer.

The invention relates to methods of determining an appropriate cancer therapy for a subject based on intratumoral expression levels of Ribonucleotide Reductase M1 (RRM1) and thymidylate synthase (TS) gene expression, as well as to methods of predicting clinical outcome (prognosis) based on cytoplasmic levels of TS protein. Compositions and kits useful for the methods are also provided.
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Neurodegenerative Disease Treatment Using JAK/STAT Inhibition

The invention relates to treatment of neurodegenerative diseases with JAK/STAT pathway inhibitors to eliminate extracellular cell signaling events leading to cell cycle abrogation and/or apoptosis. Primary neurons were administered neurotoxic proteins, such as gp120, Tat, or gp120 and Tat, with or without IFN-[gamma] added, resulting in neuronal death, and simulated neurodegenerative diseases. The neurodegenerative disease is treated using a JAK/STAT pathway inhibitor, including (-)-epigallocate
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Materials and methods for diagnosis, prevention and/or treatment of stress disorders and conditions associated with abeta peptide aggregation

The subject invention concerns materials and methods for treating and/or preventing diseases associated with accumulation of A[beta] peptide in neural tissue. The subject invention also concerns materials and methods for treating and/or preventing stress disorders, such as post-traumatic stress disorder (PTSD). In one embodiment, a method of the invention comprises administering a therapeutically effective amount of cotinine, or a pharmaceutically acceptable salt thereof, to a person or animal i
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Methods of Detecting and Treating Colon Disorders

The subject invention pertains to a method of detecting colon disorders including inflammatory bowel disease, such as Crohn's disease and ulcerative colitis, based on underexpression of Lipopolysaccharide Responsive Beige-like Anchor (LRBA). Advantageously, the invention may be used to test for inflammatory bowel disease using a blood sample from a subject before a more invasive test for colon disorders is employed. The invention also pertains to methods of treating a colon disorder in a subject
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N,n'-di-p-bromophenyl Guanidine Treatment for Stroke At Delayed Timepoints

1,3 di-o-tolylguanidine (DTG) was examined as anti-stroke drug with a broad therapeutic window. DTG activates sigma 1 and 2 receptors. Administration of DTG at 24 hours post- stroke to rats reduces neurodegeneration by 85%; this is the only pharmacological agent that has been used successfully at this delayed timepoint. Treatment with DTG provides protection of neurons exposed to hypoxia and blocks activation of immune cells that are responsible for delayed neurodegeneration associated with stro
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Methods of Treating Stroke Using Stem Cell-like Menstrual Blood Cells

A cell type that is a complete match of the transplant recipient appears as an optimal scenario to open treatment options to a large patient population with minimal complications. The use of autologous bone marrow or umbilical cord blood has been proposed as a good source of stem cells for cell therapy. Menstrual blood is found to be another important source of stem cells. Assays of cultured menstrual blood reveal that they express embryonic like-stern cell phenotypic markers and neuronal phenot
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A Method and Composition Using a Dual Specificity Protein Tyrosine Phosphatase As an Antimalarial Drug Target

Phosphotyrosine phosphatase (PTP) encoded by PF13_0027 is a desirable drug target for the human malaria parasite Plasmodium falciparum. This PTP is critical for intraerythrocytic parasite development and invasion of erythrocytes by malaria merozoites. Mutation of the PF13_0027 gene or blocking expression of PTP function to create a PTP-null parasite severely attenuates the malaria parasite's ability to survive, making the PTP-null parasite suitable as an attenuated blood-stage parasite vaccine.
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CT Atlas of the Brisbane 2000 System of Liver Anatomy for Radiation Oncologists

The method includes the steps of obtaining atlas data in an atlas coordinate set from a computer-readable atlas of hepatic anatomical information including three orders of division. The three orders of division include a first order separating the liver into two (right and left) hemi-livers, a second order transverse to the first order and dividing the liver into (anterior, posterior and medial) sections, and a third order (segments) transverse to the second order and approximately parallel to t
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Individualized Cancer Treatments

The invention provides for compositions and methods for predicting an individual's responsitivity to cancer treatments and methods of treating cancer. In certain embodiments, the invention provides compositions and methods for predicting an individual's responsitivity to chemotherapeutics, including platinum-based chemotherapeutics, to treat cancers such as ovarian cancer. Furthermore, the invention provides for compositions and methods for predicting an individual's responsivity to salvage ther
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Combination of Insulin and Ascorbate to Enhance Wound Healing

Provided is a method of stimulating collagen synthesis and proteoglycan (lumican and keratocan) accumulation. Collagenase isolated keratocytes were cultured with or without insulin with or without ascorbate. Insulin stimulates the synthesis of collagen but does not affect the accumulation of lumican and keratocan. Insulin plus ascorbate, however, stimulates the synthesis of collagen and increased the accumulation of these proteoglycans. The accumulation of PGDS, a KSPG that does not interact wit
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Method of Rapidly Visualizing Essential Neural Pathways

A method of optimizing pre-operative procedures in the field of neurosurgery. The method facilitates the identification of essential fiber tract pathways that must be avoided when planning and performing an operation, thereby increasing surgical safety and avoiding complications. The method includes the combined use of four variables in Diffusion Tensor Imaging (DTI)-Fiber Tracking (FT) of projection fibers in the brain. These variables include Jain-BremTM fractional anisotropy constant for trac
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Materials and Methods for Prevention and Treatment of RNA Viral Diseases

The subject invention concerns a method of inhibiting an RNA virus infection within a patient by increasing the amount of 2-5 oligoadenylate synthetase (2-5 AS) activity within the patient. Preferably, the preventative and therapeutic methods of the present invention involve administering a nucleotide encoding 2-5 AS, or at least one catalytically active fragment thereof, such as the p40, p69, p100 subunits, to a patient in need thereof. The present inventors have determined that overexpression
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Leukemia Treatment Method and Composition

A method of inducing high anti-leukemia activity responsive to the combination of hydroxamic acid analogue histone deacetylase inhibitors and PKC412 against human acute leukemia characterized as expressing phosphorylated (p)FLT3 kinase by a novel flow cytometry-based assay.
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Synergistic Modulation of Microglial Activation by Nicotine and THC

Treatment of microglial cells with nicotine and THC synergistically attenuate the microglial activation. Using microglial activation, the combination of THC and nicotine interact synergistically reduced LPS induced TNF-[alpha] release, showing that the combination of THC and nicotine clinically have greater efficacy in reducing neuroinflammation with less side effects than either drug given alone. CD40 signaling was found critically involved in pathological activation of microglial cells. This i
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Materials and Methods for Capture of Pathogens and Removal of Aurintricarboxylic Acid from a Sample

The present invention concerns methods and materials for extracting infectious pathogens from a sample, such as blood, comprising the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The fibrin lysis reagent is preferably composed of plasminogen and streptokinase frozen in coincident relation until the fibrin lysis reagent is needed whereby streptokinase enzymatically reacts with plasminogen to form plasmin
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Methods of Treating Cognitive Impairment

The subject invention concerns materials and methods for treating a person or animal having cognitive impairment. In one embodiment, the method comprises administering an effective amount of one or more inflammatory mediator(s), for example, fms-related tyrosine kinase 3 (Flt3) ligand, interleukin-6 (IL-6), macrophage migration inhibitory factor (MIF), interleukin-1 (IL-1), interleukin-3 (IL-3), erythropoietin (EPO), vascular endothelial growth factor A (VEGF-A), hypoxia-inducible transcription
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Tissue Classification Method for Diagnosis and Treatment of Tumors

The present invention discloses an informational computation method for classifying objects Specifically, the invention is a system, method, and computer-readable media for classifying tumors using a nonparametric statistical classifier in conjunction with an artificial neural network. The invention classifies unknown tumor types based on the correlation of unknown tumor's genetic expression compared to the genetic expression of know tumor types by first performing a nonparametric statistical an
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Ship-deficiency to Increase Megakaryocyte and Platelet Production

A method of increasing megakaryocytes and platelet numbers in a patient comprising the step of inhibiting SHIP expression, including SHIP's enzymatic activity and signaling functions, whereby normal blood clotting is induced.
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Anticancer Combination Therapy Including Triciribine

This invention encompasses combination therapies including TCN, TCN-P, TCN-PM and/or related compounds and one or more additional anti-cancer agents, for example, taxanes a molecule that modulates the HER2/neu (erbB2) receptor, anthracyclin compounds, epidermal growth factor receptor inhibitor compounds, one or more platinum compounds and bortezomib and derivatives thereof and compositions with reduced toxicity for the treatment and prevention of tumors, cancer, and other disorders associated wi
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Method of Treating Peripheral Nerve Sensory Loss Using Compounds Having Nicotinic Acetylcholine Receptor Activity

Methods for treatment of peripheral nerve sensory loss are disclosed. The methods involve treating a patient with a compound having nicotinic acetylcholine receptor activity.
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Sterile Inflatable Drape

A sterile inflatable drape includes a base having a size and shape adapted to cover a surgical site. A peripheral border surrounds the base. The base and the peripheral border have a first side covered with a releasable adhesive adapted to releasably engage a patient's skin when the base and peripheral border are placed over the surgical site prior to an incision. An expandable housing is mounted to a second side of the border in surrounding relation to the base and a hollow interior is defined
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Reatment of Suicidal Ideation Or Behavior Using Inhibitors of Nicotinic Acetylcholine Receptors

The invention concerns methods of treating suicidal ideation or behavior in a subject in need thereof, comprising decreasing endogenous nicotinic acetylcholine receptor (nAChR) activity in the subject; therapeutic packages for treating suicidal ideation or behavior; and methods for determining the efficacy of a treatment for suicidal ideation or behavior. In some embodiments, the treatment methods comprise administering to the subject an effective amount of an inhibitor of a nAChR, such as a lit
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Cancer imaging and treatment

A compound, or a pharmaceutically acceptable salt or ester thereof, comprises a ligand for the chemokine receptor CXCR4 and a detectable label, the ligand having a binding affinity for the CXCR4 receptor, measured as IC50 in the presence of <125>I-CPCR4, of 25OnM or lower, wherein the ligand comprises a cyclic oligopeptide moiety having the motif B-Arg or B- (Me)Arg within the cyclic moiety, and wherein B is a basic amino acid; a derivative thereof, or phenylalanine, provided that the motif is B
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Trocar Tube, Trocar, Obturator, Or Rectoscope for Transluminal Endoscopic Surgery Via Natural Body Cavities

The present invention relates to a trocar tube, trocar, obturator, rectoscope for transluminal endoscopic surgery via natural body cavities. A trocar tube (2) according to the invention is provided, having a distal section (22) for positioning at least in the region of or outside the body cavity, and a proximal section (21) for positioning within the human or animal, wherein at least the proximal section has a substantially rigid or inflexible curvature. The invention further provides an obturat
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Radiolabeled Monomeric and Multimeric Cyclic Oligopeptides Binding to the CXCR4 Receptor for Cancer Imaging and Treatment

A compound, or a pharmaceutically acceptable salt or ester thereof, comprises the structure: [(P1-S1j)p - L - (S2q-P2)r]t wherein: P1 and P2, which may be the same or different, are cyclic oligopeptide moieties, at least one of P1 and P2 having the motif B-Arg or B-(Me) Arg within the cyclic moiety, wherein B is a basic amino acid, a derivative thereof, or phenylalanine or a derivative thereof; S1 and S2 are spacer groups, which may be the same or different; L is a linker moiety containing at le
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Fibroblast growth factor (fgf23) and methods for use

The invention relates to nucleic acids encoding a fibroblast growth factor-23 (FGF23) and proteins encoded thereby, mutations in which are associated with autosomal dominant rickets (ADHR). The invention further relates to methods of diagnosing and treating hypophosphatemic and hyperphosphatemic disorders comprising inhibiting or stimulating, respectively, the biological activity of FGF23 in a patient. The invention also relates to methods of treating osteoporosis, dermatomyositis, and coronary
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Minimized Small Peptides with High Affinity for Factor VIII and Factor VIII-like Proteins

The present invention relates to the composition of small molecules and their use in the field of protein isolation, purification, stabilizing and/or enhancing its activity. In particular, the present invention relates to the synthesis and optimization of compounds comprising small peptides and peptide derivatives with affinity to coagulation Factor VIII and/or Factor VIII-like polypeptides and/or domains thereof. These compounds are useful for labeling, detecting, identifying, isolating and pre
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Trocar Tube, Trocar, Obturator, Or Rectoscope for Transluminal Endoscopic Surgery Via Natural Body Cavities

The present invention relates to a trocar, trocar, obturator, Rektroskop for the transluminal endoscopic surgery via natural orifices. A trocar according to the invention is provided with a distal portion for positioning at least in the region or outside the body opening and a proximal portion for positioning within the human or animal, wherein at least the proximal portion has a substantially dimensionally stable andverbiegefeste curvature. Continue to advocate the obturator adapted and customi
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Multiple N-methylated Cyclic Hexapeptides for Treatment of Neurogenic Inflammation

The present invention relates to cyclic hexapeptides containing two or more N-alkyiations in their backbone. The described cyclopeptides are active as anti-inflammatory agents and/or analgesics and may be used for the treatment of neuropathic pain and/or neurogenic inflammation.
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Use of an Ozone/oxygen Mixture As Primary Anticancer Therapy Through Intraperitoneal Insufflation

Squamous cell carcinomas of the ENT area (HNSCC) are a group of metastatic tumors with a high mortality rate in humans and animals. Since then discovered that the biomolecule ozone inhibits the growth of various cancer cells in vitro, we here applied the highly aggressive and lethal VX2 carcinoma HNSCC tumor model of New Zealand rabbits to test whether ozone exerts anti-tumor effects in vivo.Therapeutic insufflation of a medical Ozon-/Sauerstoff (O3/O2)-gas mixture in the peritoneum (O3/O2-Pneum
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Methods for the Treatment of Viral Conditions

The invention is directed to a method of stimulating PRF in a viral cell by administering an aminoglycoside antibiotic to said cell. In another embodiment, the invention is directed to a method of inhibiting viral replication by administering an aminoglycoside antibiotic to a viral cell. The invention is also directed to method of treating a viral infection in a patient suffering therefrom comprising administering to said patient an aminoglycoside antibiotic.
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Methods and Compositions for Facilitating Tissue Repair and Diagnosing, Preventing, and Treating Fibrosis

Compositions and methods for facilitating tissue repair by enhancing the actions of BIGH3 or at least one of its downstream effector molecules in injured tissue, and for the diagnosis, prophylactic and therapeutic treatment of fibrosis by inhibiting the actions of BIGH3 or at least one of its downstream effector molecules, such as PU.1 transcription factor and MMP14. Other disclosed methods include methods of screening and/or identifying compounds useful for facilitating tissue repair, treating
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Aminoflavone (nsc 686288) and Combinations Thereof for Treating Breast Cancer

Disclosed are methods of treating breast cancer by administering to a mammal aminoflavone, and optionally one or more additional anti-cancer agents. According to example embodiments, the methods include methods of treating cancers resistant to endocrine therapy.
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Methods of Treating a Microbial Infection By Modulating RNase-L Expression And/or Activity

The invention relates to methods and compositions for treating a microbial infection. In the present invention, RNase-L activity has been shown to play an integral role in innate immunity and for defense against invading microbes. The present invention is drawn to exploiting the role of RNase-L in innate immunity for methods of treating a microbial infection. The present invention is also drawn to exploiting the role of RNase-L in innate immunity for methods of treating an immune related disease
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Small Molecule Inhibitors of Lck SH2 Domain Binding

The present invention provides, materials and methods for modulating an immune response. The materials and methods may be used to treat diseases associated with an aberrant immune response. In some embodiments, materials and methods of the invention may be used to treat autoimmune diseases, for example, rheumatoid arthritis.
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Inhibitors of NCCa-ATP Channels for Therapy

Methods and compositions are provided that are utilized for treatment and/or prevention of intraventricular hemorrhage or progressive hemorrhagic necrosis (PHN), particularly following spinal cord injury. In particular, the methods and compositions are inhibitors of a particular NCca-ATP channel and include, for example, inhibitors of SURl and/or inhibitors of TRPM4. Kits for treatment and/or prevention of of intraventricular hemorrhage or progressive hemorrhagic necrosis (PHN), particularly fol
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Potent Inhibitory Effect of Zinc in Combination with Sulforaphane on Cancer Cell Growth

This invention relates to compositions comprising zinc and sulforaphane which can be used in the treatment or prevention of cancer. The invention also relates to methods of treating or preventing cancer, including prostate cancer, which comprises the administration of zinc and sulforaphane to a patient in need thereof.
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Methods for Regulating Urinary Potassium

The internalization sequence at the C-terminal end of the ROMK protein has been identified and sequenced. It has been discovered determined that endocytosis is triggered by binding of the ROMK internalization sequence to ARH protein, which is co-localized and expressed with ROMK. New methods of treating or preventing hyperkalemia have been discovered that include administering to a patient who is at risk of developing hyperkalemia or who has hyperkalemia, a therapeutically effective amount of an
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Compositions and Methods of Inducing Endoplasmic Reticulum Stress Reponse

The present invention provides methods of inducing cell cycle arrest and/or cell growth inhibition, with the methods comprising administering to the cells an effective dose of the compounds of the present invention.
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Docosahexaenoic Acid for the Treatment of Heart Failure

There is currently no completely effective treatment for heart failure. Considering the need for, and current void in the medical field for, a treatment for heart failure, the invention is drawn to treating heart failure. In particular aspects, the invention is drawn to the discovery that certain polyunsaturated fatty acids (PUFAs) and doses thereof are useful for treating heart failure. In other particular aspects, the invention is drawn to the discovery that certain PUFAs and doses thereof are
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Methods of Treating a Diabetic Embryopathy

Maternal diabetes can lead to a developmental malformation of an embryo. A developmental malformation caused by maternal diabetes is commonly referred to as a diabetic embryopathy. There is currently no effective treatment for reducing or inhibiting a diabetic embryopathy. To this end, the present invention is drawn to novel methods of treating a diabetic embryopathy.
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Methods for Treating Central Pain Syndrome and Other Pain Related Pathologies

Central pain syndrome (CPS) is a debilitating condition that affects a large number of patients with a primary lesion or dysfunction in the central nervous system. Despite its discovery over a century ago, the pathophysiology underlying the development and maintenance of CPS is poorly understood. The present invention is drawn to novel methods of treating CPS. In certain aspects, the invention is drawn to the novel discovery of the role of the zona incerta (ZI) in CPS and methods of exploiting t
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EGRF and PAR2 Regulation of Intestinal Permeability

The present invention provides a method of treating an autoimmune disease, comprising the steps of increasing transepithelial electrical resistance leading to decreased cell permeability. Further provided is ajnethod of treating celiac disease in an individual in need of such treatment, comprising the steps of: administering an antibody directed against single chain zonulin thereby inhibiting epidermal growth factor receptor and inhibiting PAR2.
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Derivatives of APF and Methods of Use

Derivatives of a novel antiproliferative factor comprising a glycopeptide is disclosed. In specific embodiments, the novel antiproliferative factor is associated with the bladder. Compositions, diagnostic kits and reagents, and methods of using the compounds for identifying and/or treating interstitial cystitis and cancer are disclosed. In particular embodiments, the glycopeptide comprises D-proline.
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In Vivo Biofilm Infection Diagnosis and Treatment

The present invention relates to a method for in vivo detection of a biof?lm infection residing in a mammal, the method comprising (i) administering to the mammal a diagnostic-effective amount of a biofilm-specific probe, wherein the probe comprises a bio film-targeting moiety and a paramagnetic nanoparticle core; and (ii) imaging the mammal to detect the presence of the biof?lm infection by observing the mammal using a magnetic resonance diagnostic technique after the biofilm-specific probe has
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Method of Treating Organophosphorous Poisoning

The present invention is directed to various methods for treating organophosphorus poisoning in an animal that is at risk of exposure to an organophosphorus compound or preventing organophosphorus poisoning in an animal that has been exposed to an organophosphorus compound, by administering a therapeutically effective amount of galantamine or a salt thereof, or a biologically active analog, derivative, fragment or variant thereof.
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Derivatives of Apf and Methods of Use

Derivatives of bladder epithelial antiproliferative factor and methods of using them are disclosed. In specific embodiments, the glycopeptide compositions are useful for the treatment and/or prevention of medical conditions, including cancer. In other embodiments, there are compositions and methods related to treatment of bladder conditions. In particular embodiments, the glycopeptide comprises D-pipecolic acid or L-pipecolic acid.
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Sur1 Inhibitors for Therapy

Methods and compositions are provided that are utilized for treatment and/or prevention of intraventricular hemorrhage or progressive hemorrhagic necrosis (PHN). In particular, the methods and compositions are inhibitors of a particular NCca-ATP channel and include, for example, inhibitors of SUR1 and/or inhibitors of TRPM4. Kits for treatment and/or prevention of intraventricular hemorrhage or progressive hemorrhagic necrosis (PHN), particularly following spinal cord injury, are also provided.
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Monoclonal Antibodies for Treatment of Cancer

The present invention provides antibodies useful as therapeutics for treating and/or preventing diseases associated with cells expressing GT468, including tumor-related diseases such as breast cancer, lung cancer, gastric cancer, ovarian cancer, and hepatocellular cancer.
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Monoclonal Antibodies Against Claudin-18 for Treatment of Cancer

The present invention provides antibodies useful as therapeutics for treating and/or preventing diseases associated with cells expressing CLDl 8, including tumor-related diseases such as gastric cancer, esophageal cancer, pancreatic cancer, lung cancer, ovarian cancer, colon cancer, hepatic cancer, head-neck cancer, cancer of the gallbladder, and the metastases thereof.
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Identification of Tumor-associated Markers for Diagnosis and Therapy

The invention relates to genetic products the expression of which is associated with cancer diseases. The invention also relates to the therapy and diagnosis of diseases in which the genetic products are expressed or aberrantly expressed, in particular cancer diseases.
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Use of RNA for Reprogramming Somatic Cells

The present invention provides methods for de-differentiating somatic cells into stem-like cells without generating embryos or fetuses. More specifically,-the present invention provides methods for effecting the de-differentiation of somatic cells to cells having stem cell characteristics, in particular pluripotency, by introducing RNA encoding factors inducing the de-differentiation of somatic cells into the somatic cells and culturing the somatic cells allowing the cells to de-differentiate.
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Methods Involving MS4A12 and Agents Targeting MS4A12 for Therapy, Diagnosis and Testing

The invention relates to methods involving MS4A12 and agents targeting MS4A12 which are useful for therapy, diagnosis and testing of disease states, in particular cancer diseases. In particular, the invention relates to agents which reduce or inhibit expression or activity of MS4A12, compositions containing these agents, and methods for reducing, determining, or increasing the responsiveness of a cell towards EGF.
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Use of Flt3 Ligand for Strengthening Immune Responses in Rna Immunization

The invention relates to supplying vaccine RNA to cells. The invention relates in particular to a common use of vaccine RNA and Flt3 ligand for inducing, creating or enhancing an immune response when administered to animals (including humans).
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Methods and Compositions for Diagnosis and Treatment of Cancer

The present invention relates to the identification of nucleic acid and amino acid sequences that are characteristic of tumor tissues such as ovarian tumor and lung tumor tissues and which represent targets for therapy or diagnosis of tumor diseases in a subject.
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Identification of Tumor-associated Markers for Diagnosis and Therapy

The invention relates to genetic products the expression of which is associated with cancer diseases. The invention also relates to the therapy and diagnosis of diseases in which the genetic products are expressed or aberrantly expressed, in particular cancer diseases.
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Monoclonal Antibodies for Treatment of Cancer

The present invention provides antibodies useful as therapeutics for treating and/or preventing diseases associated with cells expressing GT468, including tumor-related diseases such as breast Cancer, lung Cancer, gastric Cancer, ovarian Cancer, hepatocellular Cancer, colon Cancer, pancreatic Cancer, esophageal Cancer, head & neck Cancer, kidney Cancer, in particular renal cell Carcinoma, prostate Cancer, liver cancer, melanoma, sarcoma, myeloma, neuroblastoma, placental choriocarcinoma, cervica
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Vaccine Composition Comprising 5'-cap Modified RNA

The present invention relates to modification of RNA with 5 '-cap analogs in order to improve the stability and increase the expression of said RNA, in particular in immature antigen presenting cells. The present invention provides a vaccine composition comprising said stabilized RNA, immature antigen presenting cells comprising said stabilized RNA, and methods for stimulating and/or activating immune effector cells and for inducing an immune response in an individual using said stabilized RNA.
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Antibodies Specific for Claudin 6 (CLDN6)

The present invention provides antibodies useful as therapeutics for treating and/or preventing diseases associated with cells expressing Claudin-6 (CLDN6), including tumor-related diseases such as ovarian cancer, lung cancer, gastric cancer, breast cancer, hepatic cancer, pancreatic cancer, skin cancer, malignant melanoma, head and neck cancer, sarcoma, bile duct cancer, cancer of the urinary bladder, kidney cancer, colon cancer, placental choriocarcinoma, cervical cancer, testicular cancer, an
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Delivery of Transthyretin Across the Blood-brain Barrier As a Treatment for Alzheimer's Disease

The invention provides a composition comprising transthyretin and an ICAM-1 targeting agent, wherein the transthyretin and ICAM-1 targeting agent are coupled together, as well as methods of preparing such compositions. The invention further provides a diabody capable of binding specifically to ICAM-1 and transthyretin. The invention also provides a method of use of such composition in the manufacture of a medicament for treating an amyloid-ss related neurodegenerative disease, comprising adminis
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Advanced Functional Biocompatible Foam Used As a Hemostatic Agent for Compressible and Non-compressible Acute Wounds

A sprayable polymeric foam hemostat for both compressible and non-compressible (intracavitary) acute wounds is disclosed. The foam comprises hydrophobically-modified polymers, such as hm-chitosan, or other amphiphilic polymers that anchor themselves within the membrane of cells in the vicinity of the wound. By rapidly expanding upon being released from a canister pressurized with liquefied gas propellant, the foam is able to enter injured body cavities and staunch bleeding. The seal created is s
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Identification of tumor-associated antigens for diagnosis and therapy

The invention relates to genetic products the expression of which is associated with cancer diseases. The invention also relates to the therapy and diagnosis of diseases in which the genetic products are expressed or aberrantly expressed, in particular cancer diseases.
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Methods and Compositions for Inhibiting Angiogenesis

The present invention relates to methods and compositions for modulating angiogenesis. In particular, the present invention relates to Pigment Epithelial-derived Factor (PEDF) fragments for use in modulating angiogenesis and treating angiogenesis mediated disease.
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Compositions and Methods for Treatment, Research and Therapeutic Applications for Malaria

The present invention provides anti-Plasmodium immunogenic compositions comprising EVP1 (PFD0495c) or an antigenic portion thereof, as well as methods of immunizing against malaria employing these compositions. In other embodiments, the present invention provides methods of identifying Plasmodium infection employing agents that bind to EVP1 or an antibody generated thereto.
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Method of Regulating the Heat Shock Response

The present invention is directed to method of modulating a heat shock response in a first cell of a multicellular organism comprising stimulating or inhibiting an HSR signaling activity of a second cell, wherein the second cell is a neuronal cell that regulates heat shock response activation in the first cell and that does not directly innervate the first cell.
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Compositions and Methods for Treating Atrial Fibrillation

The present invention provides compositions and methods for treating heart conditions. In particular, the present invention provides compositions and methods that block G protein coupled receptor mediated signaling for treating atrial fibrillation.
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Compositions and Methods for Treating Bone Formation Disorders

The present invention relates to compositions and methods for the detecting, treating, and empirically investigating disorders associated with bone formation (e.g., osteoporosis). In particular, the present invention provides compositions and methods for using maspin and targets of maspin in the diagnosis, treatment, and empirical investigation of disorders associated with bone formation (e.g., osteoporosis).
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Delivery Scaffolds and Related Methods of Use

The present invention relates to delivery systems. In particular, the present invention provides microporous scaffolds having thereon agents (e.g., extracellular matrix proteins, exendin-4) and biological material (e.g., pancreatic islet cells). In some embodiments, the scaffolds are used for transplanting biological material into a subject. In some embodiments, the scaffolds are used in the treatment of diseases (e.g., type 1 diabetes), and related methods (e.g., diagnostic methods, research me
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Methods and Compositions for Modulating Calcium Channels

The present invention relates to methods and compositions for modulating calcium channels. In particular, the present invention provides methods and compositions for modulating (e.g., disrupting) Cav1.3a calcium channels for research and therapeutic methods (e.g., treating dopaminergic diseases and conditions).
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Use of SCF and G-CSF in the Treatment of Cerebral Ischemia and Neurological Disorders

The present invention relates to the use of stem cell factor (SCF) polypeptide, alone and in combination with granulocyte colony stimulating factor (G-CSF) polypeptide, in the prevention or treatment of injury to the brain after cerebral ischemia or neurological disorder. More particularly, the invention provides methods of improving neurological function and outcome after stroke by the administration of SCF polypeptide, alone and in combination with G-CSF polypeptide. This treatment can be used
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Cardiac Pacing Lead and Delivery Sheath

The present invention provides compositions and methods related to epicardial pacing systems. In particular, the present invention provides a novel delivery sheath and cardiac pacing lead to be deployed in the pericardial space via a percutaneous approach.
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Method for Removal Or Inactivation of Heparin

The present invention relates to the use of immobilized RAGE or portions thereof for removal of heparin and low molecular weight heparin from a fluid sample or from a patient in need of neutralization of anticoagulant activity. The invention provides a method for removal or inactivation of heparin and low molecular weight heparin, as well as a device which utilizes this method.
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Pathogenecity Islands of Pseudomonas Aeruginosa

Disclosed are Pseudomonas aeruginosa Genomic Island nucleic acid sequences referred to as PAGI-5, PAGI-6, PAGI-7, PAGI-8, PAGI-9, PAGI-10, and PAGI-11. These nucleic acid sequences may be useful in methods for identifying virulent strains of Pseudomonas bacteria.
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Cardioprotective Role of Hepatic Cells and Hepatocyte Secretory Factors in Myocardial Ischemia

The present invention provides methods, compositions, and systems for treating a subject at risk for, with, or suspected of having, myocardial ischemia using hepatocyte secretory factors (e.g., AGP2, BMPER, FGF21, NRG4, and/or TFF3) or using factors that promote liver cell migration to ischemic myocardial tissue (e.g.,IL-6 and/or MMP-2).
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Sealants for Membrane Repair

The present invention provides a method for preventing or repairing damage to a fetal membrane. In one embodiment, the method comprises contacting a fetal membrane with a composition comprising a four-armed catechol-terminated polyethylene glycol (cPEG) and a biocompatible oxidant, hi one embodiment, the four-armed cPEG and the biocompatible oxidant are initially contained in separate solutions, and the solutions are mixed to form the composition just prior to or at the same time that the compos
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Polyvalent Polynucleotide Nanoparticle Conjugates As Delivery Vehicles for a Chemotherapeutic Agent

The present invention is directed to compositions and methods of delivering a chemotherapeutic agent via a polynueleotide-functionalized nanoparticle (PN-NP).
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Methylation Profile of Neuroinflammatory Demyelinating Diseases

The present invention relates to compositions and methods for diagnosing neuroinflammatory demyelinating diseases, including but not limited to, multiple sclerosis. In particular, the present invention provides methods of identifying methylation patterns in genes associated with neuroinflammatory demyelinating diseases.
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Compositions and Methods for Urinary Bladder Regeneration

The present invention provides compositions and methods for the regeneration of tissue. In particular, the present invention provides mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) for bladder regeneration.
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Live-attenuated Compositions for Bacterial Infections

The present invention relates to compositions and methods for disease treatment and prevention through administration of a live attenuated composition. In particular, the present invention provides compositions and methods for the treatment and prevention of urinary tract infection by administration of a live attenuated compositions lacking O-antigen ligase activity.
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Compositions and Methods for Treating Conditions Associated with Neuronal Dysfunction

The present invention relates to compositions and methods for the treating and empirically investigating conditions associated with neuronal dysfunction (e.g., chronic pain, epileptic neuronal activity). In particular, the present invention provides compositions and methods for using flufenamic acid in the treatment and empirical investigation of conditions associated with neuronal dysfunction (e.g., chronic pain, epileptic neuronal activity).
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Surface Independent, Surface-Modifying, Multifunctional Coatings and Applications Thereof

The invention provides surface treatments that reduce or eliminate marine biofouling of various surfaces. A surface that is to be subjected to a marine environment can be treated with a mPEG-DOPA. The treated surface is thus rendered less susceptible to fouling of the surface.
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Method for Reducing Scarring During Wound Healing Using Antisense Compounds Directed to CTGF

This invention provides a method for reducing hypertropic scarring resulting from dermal wound healing in a subject in need which comprises administering to the subject an antisense oligonucleotide which inhibits expression of connective tissue growth factor (CTGF) in an amount effective to inhibit expression of CTGF and thereby reduce hypertrophic scarring.
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Methods for Scar Prevention

Provided herein are compositions and methods for preventing or reducing scar formation (e.g., hypertrophic scars). For example, provided herein are methods of administrating HMG-CoA-inhibiting agents for preventing or reducing scar formation.
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Compositions and Methods for Treating Lymphoma

Provided are compositions comprising PX-478, or metabolites, analogs or derivatives thereof for treatment of lymphoma.
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Use of ECDI-fixed Cell Tolerance As a Method for Preventing Allograft Rejection

The present invention provides methods, systems, and compositions for inducing donor-specific tolerance. In particular, the present invention provides methods of administering ECDI-treated cells before, during, and/or after administration of donor transplant cells or a donor allograft in order to induce tolerance for the cells and/or allograft in a recipient.
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Methods, Compositions and Articles of Manufacture for Contributing to the Treatment of Cancers

Methods, compositions and articles of manufacture for contributing to the treatment of cancers, including solid tumors, are disclosed. The methods, compositions and articles of manufacture can utilize an endothelin B agonist (ETB) to enhance the delivery and resulting efficacy of a chemotherapeutic agent.
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Anticoagulant Antagonist and Hemophilia Procoagulant

A method for treating a coagulation deficient patient comprises administering an amount of polyP to the patient sufficient to reduce the PT Test value or Dilute PT Test value of the plasma of the patient.
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Minimally-invasive Measurement of Esophageal Inflammation

The methods and apparatus of the present invention allow the evaluation of inflammation of the esophagus. Measurements may be utilized, for example, to diagnose a disease of the esophagus, to monitor inflammation of the esophagus, or to access the treatment of a disease of the esophagus. In one embodiment, the invention comprises a method for measuring esophageal inflammation comprising deploying a device into the esophagus of a subject, removing the device after a predetermined period of time,
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Fibrin Sealant

A fibrin sealant, comprises (a) thrombin, (b) fibrinogen, (c) polyP, and (d) calcium. The thrombin and the fibrinogen are separated prior to application.
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Encapsulated Materials and Methods for Encapsulating Materials

A system that incorporates teachings of the present disclosure may include, for example, an apparatus having an outer nozzle operable to discharge an outer stream of a shell solution, and an inner nozzle operable to discharge an inner stream of a core solution intermixed with a plurality of materials. The outer stream can substantially surrounds the inner stream, thereby forming a combined stream. A plurality of capsules can be formed responsive to a force applied to the combined stream. At leas
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Compositions and Methods for Treating Pulmonary Hypertension

Provided herein are methods and materials for treating pulmonary hypertension (PH) in a subject. Also provided herein is a method of diagnosing whether a has PH by detecting a PH marker. A PKG pulmonary hypertension marker has been identified and may be useful in predicting PH disease progression and assessing a subject's response to PH therapy.
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Compositions Comprising Decitabine and Tetrahydrouridine and Uses Thereof

Compositions comprising decitabine and tetrahydrouridine for the treatment of blood disorders and hematological and solid malignancies are described.
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Functional Role for Cannabinoids in Autonomic Stability During Sleep

The present invention relates generally to pharmacological methods for the prevention or amelioration of sleep-related breathing disorders via administration of cannabimimetic agents and combinations thereof.
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Method of Treating Neurological Diseases

A method of treating neurological diseases, like Alzheimer's Disease by administering a compound that activates protein kinase C and inhibits histone deacetylases to an individual suffering from the neurological disease. The method utilizes benzolactam compounds that reduce Ass production and blocks oxidative stress in the treatment of Alzheimer's disease. The benzolactam compounds and compositions containing the benzolactam compounds also are disclosed.
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Phospholipid Micellar and Liposomal Compositions and Uses Thereof

The invention generally relates to compositions and methods for the reduction or neutralization of toxins associated with a bacterial, mycobacterial, fungal, viral, or protozoal agent. More particularly, the invention is directed to sterically stabilized phospholipid micellar and liposomal compositions, which interact with the toxins to decrease or neutralize their toxicity. Additionally, the invention includes the use of sterically stabilized phosholipid micellar compositions comprising one or
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Modulation of Platelet Aggregation

Methods and compositions for inhibition of platelet cell aggregation are described. In particular, compositions comprising cell permeant RGT peptides, such as RGT bound to a lipid moiety are provided. Compositions may be used in the treatment and prevention of clot related diseases such as stroke and myocardial infarction.
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Methods and Compositions for the Inhibition of Thrombus Formation

The present invention is directed to compositions comprising inhibitors of GPIb-IX-VWF binding and methods of treating or preventing thrombosis associated with sepsis or endotoxemia in a mammal.
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Anti-bacterial Compositions and Methods Including Targeting Virulence Factors of Staphylococcus Aureus

This disclosure relates to compositions and methods including for the inhibition, prevention, and/or treatment of microbial infections, including infections from such pathogens as Staphylococcus aureus.
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Method and Composition for Treating Alzheimer's Disease and Dementias of Vascular Origin

A composition and method of treating Alzheimer's disease or a dementia of vascular origin are disclosed. The composition and method utilize an endothelin antagonist as the active agent to treat Alzheimer's disease or a dementia of vascular origin in mammals, including humans.
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Compositions and Methods for Treating a Disease Mediated By Soluble Oligomeric Amyloid Beta

The present invention relates to methods for restoring fast axonal transport in a cell affected by oligomeric amyloid beta and for treating an oligomeric amyloid beta- mediated disease such as Alzheimer's disease using a Casein Kinase 2 inhibitor and, in some embodiments, a Glycogen Synthase Kinase 3 inhibitor.
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Compositions and Methods for the Treatment of Myocardial Dysfunction Associated with Sirs Or Sepsis

Provided are compositions and methods for the treatment of myocardial dysfunction associated with SIRS or sepsis, which methods comprise the administration to a patient in need thereof of a composition comprising one or more adenosine deaminase (ADA) inhibitor and/or one or more xanthine oxidase (XO) inhibitor. Exemplified herein are methods for the treatment of myocardial dysfunction, which methods comprise the administration of a composition comprising the ADA inhibitor pentostatin and/or a co
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Method for Detecting the Existence of Renal Calculi And/or Inflammation of the Excretory Urinary Tracts

The invention relates to methods for detecting diseases of the excretory urinary tracts. The aim of the invention is to provide a method for detecting the existence of diseases of the excretory urinary tracts, which can be carried out non-invasively, thus allowing first indications of disease, e.g. in relation to the existence of renal calculi and/or inflammation (e.g. after urinary tract infections) of the excretory urinary tracts, to be rapidly and cost-effectively detected, in order to resolv
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Method of Determining Sensitivity of Human Or Non-human Animal Cells to an IAP Antagonist

cFLIP serves as a biomarker for efficacy of treatment with IAP antagonists, including Smac peptidomimetics.
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X-ray Marker for Visually Comparing and for Controlling the Image Processing in Digital X-ray Recordings, in Particular for a Thorax X-ray Recording for a Patient Lying Down

The invention relates to an X-ray marker (1) that is also to be X-rayed in an X-ray recording of an organ of a patient (3) to be examined. The X-ray marker (1) has such a geometric X-ray weakening structure, so that the associated X-ray image (R) approximately shows the expected X-ray image structure of the organ to be examined after photographic development of an X-ray film, after digitalization and digital image processing of an X-ray storage film (6), or after digital recording and image proc
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Device for Determining Vertebral Distances of the Spinal Column of a Patient

The invention relates to devices for determining vertebral distances of the spinal column of a patient. The devices are characterized in particular in that vertebral distances of the patient while walking in a building or outdoors can be easily detected. For this purpose, at least two marks are applied to the skin of the patient at the height of the transverse processes while the patient is at rest, each mark being at least two lined representations arranged at a distance from each other. Furthe
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Modulation of MLCK-L Expression and Uses Thereof

In various aspects and embodiments the invention provides methods and reagents for controlling gene expression, and for treating disorders and diseases. Embodiments provide methods and reagents specifically for the regulation of MLCK expression and for the use thereof in treating disorders and diseases. Various embodiments provide methods and reagents for specifically down regulating the expression of MLCK-L more efficiently than that of MLCK-S, and for the use thereof in treating disorders and
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Methods of Treating Diastolic Dysfunction and Related Conditions

The invention provides a method of treating diastolic dysfunction, e.g., diastolic dysfunction with preserved ejection fraction, in a subject. The method comprises administering to the subject in an amount effective to treat the diastolic dysfunction a cardiac metabolic modifier comprising a structure of Formula I, as described herein. In some embodiments, the diastolic dysfunction is characterized by (i) a lack of increased late INa in cardiomyocytes, (ii) an increase in myofilament calcium sen
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Compositions and Methods for the Treatment of Krabbe and Other Neurodegenerative Diseases

Provided are compositions and methods for the treatment of Krabbe and other neurodegenerative diseases, including storage diseases such as GMl gangliosidosis, Niemann-Pick disease, Tay-Sachs disease, Sandhoff disease, metachromatic leukodystrophy, Canavan disease, Pelizaeus-Merzbacher disease, and storage conditions facilitated by aging of lysosomal functions, which are associated with psychosine (and/or other storage material)- -mediated axonal degeneration. Compositions and methods employ (1)
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Method and Composition for Potentiating an Opiate Analgesic

Composition and methods of treating pain and reducing or reversing tolerance to opiate analgesics are disclosed. The composition and method utilize an opiate analgesic and an endothelin antagonist as active agents to treat pain in mammals, including humans.
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Hdac Inhibitors and Therapeutic Methods Using the Same

Histone deacetylases inhibitors (HDACIs) and compositions containing the same are disclosed. Methods of treating diseases and conditions wherein inhibition of HDAC provides a benefit, like a cancer, a neurodegenerative disorder, a neurological disease, traumatic brain injury, stroke, malaria, an autoimmune disease, autism, and inflammation, also are disclosed.
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Antimicrobial Therapy for Bacterial Infections

The disclosure provides compounds and methods to treat bacterial pathogenesis, and demonstrates that the S. aureus pigment is a virulence factor and potential novel target for antimicrobial therapy.
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Compositions and Methods for Inhibiting Entry of a Hepatic Virus

The present invention embraces Niemann-Pick C1-like 1 protein antagonists and agents that inhibit hepatic virus infection for use in the prevention and treatment of a hepatic virus infection.
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Materials and Methods for Making Improved Micelle Compositions

Provided are methods of treatment of many different diseases and disorders using micelle and sterically stabilized crystalline compounds of the invention.
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Polymerized Hemoglobin Media and Its Use in Isolation and Transplantation of Islet Cells

Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells
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Self-assembled Toroidal-spiral Particles and Manufacture and Uses Thereof

Toroidal-spiral shaped particles, their method of manufacture, and uses thereof are disclosed. The toroidal-spiral particles can contain at least one active agent, such as a drug, and provide a controlled, sustained release of the active agent.
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Methods and Compositions for Treating Brain Diseases

The present disclosure provides targeting peptides and vectors containing a sequence that encodes targeting peptides that deliver agents to the brain.
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Bioactive Sutures for the Treatment of Cancer

The present invention relates to compositions and method for the treatment of cancer using a suture or dressing providing a plurality of tumor cell antigens and/or an immunostimulatory agent to a subject in need of such treatment.
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RCA Locus Analysis to Assess Susceptibility to AMD and MPGNII

The invention relates to gene polymorphisms and genetic profiles associated with an elevated or a reduced risk of alternative complement cascade deregulation disease such as AMD and/or MPGNII. The invention provides methods and reagents for determination of risk, diagnosis and treatment of such diseases. In an embodiment, the present invention provides methods and reagents for determining sequence variants in the genome of a individual which facilitate assessment of risk for developing such dise
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Prenylated Bisphosphonates As Anti-tuberculosis Agents

The invention provides methods to treat a mycobacterium infection and methods to inhibit mycobacterial polyprenyl pyrophosphate synthesis with a compound of formula I. The invention also provides novel compounds of formula I as well as salts and prodrugs thereof.
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Diagnosis and Treatment of Age-Related Macular Degeneration

The invention relates to Factor H gene polymorphisms and haplotypes associated with an elevated or a reduced risk of AMD. The invention provides methods and reagents for diagnosis and treatment of AMD.
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Method and Compositions for Treating and Preventing Seizures By Modulating Acid-sensing Ion Channel Activity

This invention provides novel methods and compositions for treating and preventing seizures by administration of ASIC1a receptor activating compounds. A novel method of assaying ASIC1a receptor activating compounds is included in the present invention. According to the invention applicants have demonstrated that seizure duration, intensity, and progression may be modulated by administration of an ASIC1a receptor activator which acts to increase the endogenous activity of ASIC1a receptors to medi
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Repeated Administration of Lentiviral Vectors to Respiratory Cells

The invention provides methods for administering lentiviral vectors to the respiratory system of a patient to treat a disease.
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Ankle Prosthesis Methods

Methods of implanting an ankle prosthesis in a subject having an ankle joint in need of replacement.
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Treatment of Hepatitis C Virus Infections

This present invention provides for a new class of HCV NS3/4A protease inhibitors as additional therapeutics for hepatitis C virus. The proposed compounds, bilverdin, bilirubin, and derivatives thereof, are based on natural enzymatic products of heme metabolism that may be more stable, better tolerated, and more resistant to mutations than present prototypic protease inhibitors.
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Use of unmethylated CpG oligonucleotides for the treatment of allergy

An immuostimulatory nucleic acid containing an unmethylated CpG dinucleotide for use in a method of treating or preventing an allergy in a subject, wherein an effective dose of the immunostimulatory nucleic acid alone is to be administered to the subject.
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Vaccine, therapeutic composition and methods for treating or inhibiting francisella tularenis

The present invention is directed to compounds and methods for treating a mammal exposed to Francisella tularensis.
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Methods of Treating Pulmonary Diseases and Disorders by Modulating Calcium/Calmodulin Dependent Protein Kinase II Activity

Disclosed are methods of treating or preventing a pulmonary disease or disorder in a subject, the methods comprising administering an effective amount of an compound that inhibits Calmodulin Kinase II (CaMKII) activity, either directly or indirectly, thereby treating or preventing the pulmonary disease or disorder in the subject.
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Method for Precisely Determining the Fluorescence in a Layer System, Such As the Eye

The invention relates to a method for precisely determining the fluorescence in a layer system, such as the eye. The object of the invention is to provide a method that is as simple and requires as little effort as possible for evaluating the fluorescence in a layer system, by means of which the summary decay behavior of the fluorescence can be evaluated very precisely, and by means of which the points of origin of the individual fluorescences of the layer system can be determined at the same ti
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Treating Cancer with Desthiazolyl Ritonavir

Treatment of cancer includes administering a compound of formula I, for example desthiazolyl ritonavir, to a subject. In particular, treatment of breast cancer is described.
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Catalytic Topoisomerase II Inhibitors

The invention provides methods and compositions useful for treating cancer, for selectively inhibiting topoisomerase II, and for inducing apoptosis.
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Ischemia/reperfusion Protection Compositions and Methods of Using.

The invention provides for ischemia/reperfusion protection compositions having one or more ketone bodies and melatonin. The invention also provides for methods of using such compositions to reduce or prevent ischemia/reperfusion injury due to blood loss, stroke or cardiopulmonary arrest or surgery.
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DNA Cytosine Deaminase Inhibitors

Cytosine deaminase inhibitors and methods for identifying inhibitors of the anti-retroviral activity of APOBEC3G are described.
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Methods of Treating HIV Patients with Anti-fibrotics

The invention relates to methods of treating patients infected with human immunodeficiency virus (HIV) with a therapeutic that has anti-fibrotic effects, for example, pirfenidone and analogs thereof.
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Decellularization and Recellularization of Organs and Tissues

The invention provides for methods and materials to decellularize an organ or portion thereof and to recellularize such a decellularized organ or portion thereof to thereby generate an organ or portion thereof.
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Methods and Materials for Isolating Isogenic Islet Cells

Compositions and methods for isolating and purifying islet cells are described. Islets obtained using such compositions and methods can be transplanted into diabetic patients.
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Methods and Systems for Treating and Preventing Cardiac Injury in Dystrophic Subjects

The present invention provides methods for treating and preventing cardiac deficiencies and injuries (e.g., cardiac injury and ventricular dilation) in dystrophic subjects using poloxamer containing compositions. In some embodiments, the polaxamers are administered to dystrophic subjects prior to cardiac stress, such as that caused by anaesthesia. In other embodiments, the present invention provides systems comprising poloxamer compositions and at least one anaesthetic agent.
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Bioresorbable Embolization Microspheres

The present disclosure is generally directed to an embolic material which is in the form of a microsphere or a plurality of microspheres. The embolic material generally comprises carboxymethyl chitosan (CCN) crosslinked with carboxymethyl cellulose (CMC). In some embodiments, the embolic material may further comprise a therapeutic agent, such as doxorubicin.
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Receptor-targeting Reagents

The present disclosure features, inter alia, receptor-targeting reagents (e.g., immunotoxic receptor-targeting reagents), which are useful in, e.g., methods of binding a receptor-targeting reagent to a cell and methods for treating a variety of disorders such as, but not limited to, cancers and inflammatory disorders. Also featured are methods, compositions, and kits useful for selecting an appropriate treatment modality for a subject (e.g., a subject with a cancer or inflammatory disorder) and/
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Method for Identifying Individual Viruses in a Sample

The problem of the invention consisted in identifying individual viruses in an arbitrary sample quickly, unambiguously and reliably, and with the least possible preparation-related and technology-related expenditure, without necessitating immobilization using antibodies and without requiring an indication or at least a suspicion of potentially present viruses. According to the invention, the height profile of the sample is scanned, from which scanning sites suspected of containing viruses are se
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Method for Identifying Individual Viruses in a Sample

The problem of the invention consisted in identifying individual viruses in an arbitrary sample quickly, unambiguously and reliably, and with the least possible preparation-related and technology-related expenditure, without necessitating immobilization using antibodies and without requiring an indication or at least a suspicion of potentially present viruses. According to the invention, the height profile of the sample is scanned, from which scanning sites suspected of containing viruses are se
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Compositions and Methods for Repair of Tissues

Biomaterials providing sustained release of growth factor for repair of tissues such as bone and cartilage are disclosed. The biomaterials comprise a proteoglycan derived from domain I of perlecan and a growth factor, and, optionally, collagen.
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Pan-Antagonists for the Androgen Receptor and Androgen Receptor Mutants Associated with Anti-Androgen Withdrawal

Disclosed herein are novel antagonists of the androgen receptor and androgen receptor mutations associated with clinical failure of currently prescribed anti-androgens and use of said antagonists in the treatment of conditions associated with inappropriate activation of the androgen receptor.
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Methods of Administering Porcine B-domainless FVIII

The present invention provides a method of administering porcine B-domainless factor VIII (OBI-1) to a patient having factor VIII deficiency to provide more rapid and effective protection against bleeding episodes, compared to formerly available methods, or to provide more effective protection to such patients during non-bleeding periods. This invention is based on the discovery that the recombinant B-domainless porcine fVIII, termed OBI-1, has greater bioavailability compared to the natural por
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Modified Factor VIII

Methods of treating patients with Factor VIII deficiency by administration of modified porcine factor VIII are disclosed. The particular modified porcine factor VIII is one in which most of the B domain has been removed through genetic engineering. This modified factor VIII is particularly useful for treatment of hemophiliacs, especially those undergoing bleeding episodes.
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Compositions and Methods for the Treatment of Infections and Tumors.

PD-1 antagonists are disclosed that can be used to reduce the expression or activity of PD-1 in a subject. An immune response specific to an infectious agent or to tumor cells can be enhanced using these PD- 1 antagonists in conjunction with an antigen from the infectious agent or tumor. Thus, subjects with infections, such as persistent infections can be treated using PD-1 antagonists. In addition, subjects with tumors can be treated using the PD-1 antagonists. In several examples, subjects can
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Nucleoside Derivatives for Treatment of Caliciviridae Infections, Including Norovirus Infections

A method and composition for treating a host infected with a Caliciviridae virus, such as a Norovirus, comprising administering an effective treatment amount of a described modified nucleoside or a pharmaceutically acceptable salt thereof, is provided.
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Vascular Access to Extra-vascular Space

Provided are methods for accessing tissue in the vicinity of a blood vessel. Also provided are devices for use in a lumen of a patient's organ and methods for shunting interior volumes of organs.
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Systems and Methods for Preventing Mistransfusion

In some embodiments, a system and a method for preventing mistransfusion of a patient pertain to obtaining a sample of the patient's blood, obtaining a sample of blood that is about to be infused into the patient, mixing the samples together, determining whether the samples are compatible, and if the samples are not compatible, automatically preventing infusion of the blood into the patient.
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Methods of Determining Renal Function using Technetium-99m Tricarbonyl-nitrilotriacetic acid

This disclosure relates to the methods of imaging the kidneys and measuring renal function using the renal tracer 99mTc(CO)3(NTA) (technetium-99m tricarbonyl-nitrilotriacetic acid). The disclosure encompasses methods of imaging the kidneys and measuring effective renal plasma flow (ERPF) in an animal or human subject using renal scintigraphy, comprising administering to an animal or human subject an amount of a renal tracer, wherein the renal tracer comprises 99mTc-(CO)3(NTA). Another aspect of
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Compositions and Methods for Treatment Or Prevention of Post-operative Organ Or Tissue Inflammation

Compositions, methods and kits for treatment or prevention of post operative organ or tissue inflammation are provided. The compositions contain an effective amount for local delivery, of an anti-inflammatory agent, alone or in combination with other pharmacologic agents, embedded within a polymeric matrix or gel. The polymeric matrix or gel may be a formed from natural or synthetic precursor components. The compositions are applied locally to an organ or tissue for the treatment or prevention o
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Methods for the Treatment of Infections and Tumors

PD-I antagonists are disclosed that can be used to reduce the expression or activity of PD-I in a subject. An immune response specific to an infectious agent or to tumor cells can be enhanced using these PD-I antagonists in conjunction with an antigen from the infectious agent or tumor. Thus, subjects with infections, such as persistent infections can be treated using PD-I antagonists. In addition, subjects with tumors can be treated using the PD-I antagonists. In several examples, subjects can
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Treatment of EBV and KHSV Infection and Associated Abnormal Cellular Proliferation

A method and composition for the treatment, prevention and/or prophylaxis of a host, and in particular, a human, infected with Epstein-Barr virus (EBV), is provided that includes administering an effective amount of a 5-substituted uracil nucleoside or its pharmaceutically acceptable salt or prodrug, optionally in a pharmaceutically acceptable diluent or excipient.
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Treating Various Disorders with 7,8-dihydroxyflavone and Derivatives Thereof

Novel compounds and methods related to the activation of the TrkB receptor are provided. The methods include administering in vivo or in vitro a therapeutically effective amount of 7,8-dihydroxyflavone or derivative thereof. Specifically, methods and compounds for the treatment of disorders including neurologic disorders, neuropsychiatric disorders, and metabolic disorders (e.g., obesity) are provided. For example, a first method is provided of treating or reducing the risk of depression, anxiet
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Methods for the Treatment of Graft-versus-host Disease

Methods are disclosed for treating or preventing graft versus host disease in a subject. The methods include selecting a subject in need of treatment for graft versus host disease; and administering to the subject a therapeutically effective amount of a TLR5 agonist such as a flagellin polypeptide, or a polynucleotide encoding the flagellin, thereby treating or preventing graft versus host disease in the subject. Methods are also disclosed for reducing susceptibility to an opportunistic infectio
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Methods for the Treatment of a Traumatic Central Nervous System Injury

Methods of treating a subject with a traumatic central nervous system injury, more particularly, a traumatic brain injury, are provided. The methods comprise a therapy comprising a constant or a two-level dosing regime of progesterone. In one method, a subject in need thereof is administered at least one cycle of therapy, wherein the cycle of therapy comprises administering a therapeutically effective two-level intravenous dosing regime of progesterone. The two-level dosing regime comprises a fi
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Methods for Preventing Oxidative Stress Injury of a Tissue By a Folate-tempol Conjugate

The present disclosure is generally related to methods of preventing oxidative stress injury induced by renal ischemia-reperfusion by a Folate-TEMPOL conjugate.
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Xenotropic Mulv-related Virus (XMRV) Compositions and Methods of Use

The invention relates to compositions and methods for analyzing and treating a disease, disorder, or condition associated with an XMRV infection.
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Small Molecule Ligand-drug Conjugates for Targeted Cancer Therapy

The present invention describes small molecule ligand-drug conjugates and methods of using the small molecule ligand-drug conjugates for targeted treatment of cancer in a patient in need thereof. Further described are methods of sterilizing circulating tumor cells and determining drug concentration in cancer tissue.
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Microparticles for Delivery to Cells And/or Tissues

Delivery of bioactive molecules (BAMs) to organelles, cells, tissues, or animals with high efficiency is desirable. Novel compositions for highly efficient delivery of bioactive molecules by biolistic methods are described. The novel compositions include novel microparticle cores, microparticle core/BAM complexes, microparticle core/nanoparticle complexes and multilayer-microparticle core/BAM complexes. Any one of these may be further modified to include targeting agents.
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Mucosal Immunization

Methods and compositions for eliciting an immune response to an antigen are disclosed. In certain aspects, these methods concern eliciting an immune response in a subject by administering to the mucosa of the subject a composition comprising a virus-like particle ("VLP") and Murabutide.
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Bispecific Nanobodies As a Therapeutic for Alzheimer's Disease

The present invention provides for the treatment of Alzheimer's disease. More specifically, a recombinant bispecific antibody fragment that simultaneously blocks beta-secretase activity while also promoting alpha-secretase activity, comprising a first portion and a second portion, wherein the first portion blocks beta secretase activity and the second portion promotes alpha-secretase activity.
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Recombinant Bacterium Capable of Eliciting an Immune Response Against Enteric Pathogens

The present invention relates to a recombinant bacterium that is capable of eliciting an immune response against at least two enteric pathogens, without substantially inducing an immune response specific to the serotype of the bacterium. The invention also relates to methods of making such a bacterium and vaccines and methods of using such a bacterium.
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Methods and Compositions for Treating Or Preventing Pruritis

The invention features therapeutic compositions comprising agents useful for the treatment or prevention of pruritis, and methods useful for identifying such agents.
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Compositions and Methods for Treating Or Preventing Radiation Injury

The invention provides compositions and methods featuring Nrf2 activators for treating or preventing radiation-associated tissue damage.
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Treatment of Chronic Inflammatory Respiratory Disorders

This invention relates, e.g., to a method for treating a subject having a chronic inflammatory respiratory disorder, comprising administering to the subject an effective amount of an inhibitor of the expression of and/or the activity of VEGF-A and/or VEGFR1 and/or VEGFR2 and/or NP1, or a combination thereof. Also described are screeing assays for agents for treating a subject having a chronic inflammatory respiratory disorder, and kits for performing one of the methods of the invention.
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Compositions and Methods of Treating Endothelial Disorders

Compositions and methods of treating endothelial disorders, including: asthma, erectile dysfunction, pulmonary hypertension, cardiovascular disorders, and other disorders, using a synergistic combination of at least one arginase inhibitor and at least one phosphodiesterase (PDE1), (PDE2) and/or (PDE5) inhibitor are described.
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Treating cancer by increasing intracellular malonyl CoA levels

A method of killing cancer cells by acute elevation of cellular malonyl Coenzyme A (Malonyl CoA) which leads to apoptosis. Elevation of malonyl CoA may be induced by inhibition of fatty acid synthase (FAS), or by other manipulation of fatty acid metabolism. Alternatively, growth of tumor cells may be inhibited by FAS inhibition in conjunction with inhibition of carnitine palmitoyltransferase-1 (CPT-1). Combination therapy with drug(s) that inhibit fatty acid synthesis by inhibiting acetyl CoA ca
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Compositions and Methods for Treating and Preventing Heart Tissue Degeneration and Uses Thereof

The present invention provides compositions useful for cardiac therapy comprising a cyclin-associated agent. The present invention also provides kits for use in delivering a cyclin-associated agent to cardiac cells in a subject, comprising the composition of the present invention and a catheter. The present invention additionally provides a methods for enhancing cardiac function; promoting regeneration of cardiac tissue; inducing endogenous myocardial regeneration; and preventing or treating hea
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Sustained Release Formulation of Melatonin

The present invention relates to low-dose formulations of melatonin, and methods of use thereof, which provide a sustained release of melatonin so as to rapidly increase plasma levels of melatonin, maintain a relatively high level (which mimics the endogenous level of a young subject) for approximately 5-6 hours, and then decrease so as to achieve low levels by early morning (rapid washout), thereby avoiding a "hangover effect". The sustained release formulations of the invention may be used to
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Inflammation-regulating Compositions and Methods

The various embodiments provide a composition that provides local control over inflammation. The composition localizes the activities of the cytokine-neutralizing antibodies to the site of inflammation through covalent attachment to hydrophilic matrices. The various embodiments including a hydrophilic polymer, a ligand binding moiety covalently attached to the polymer, and optionally, a cellular adhesion peptide covalently attached to the polymer. The hydrophilic polymer may be a glycosaminoglyc
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Antibodies and Conjugates for Modulators of Angiogenesis

We provide methods and compositions for the treatment of dysregulation of blood vessel growth by regulation of neovascularization. Embodiments accomplish this by restricting the diffusion and transport of therapeutic agents through conjugating them to polymers or polymer constructs while retaining the binding affinities and functions of the therapeutic agents.
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Method of Assessing a Body Part

A method of assessing a body part includes obtaining two or more types of data through a combination of two or more of the following four imaging modalities: (1) obtaining surface topography data of the body part, (2) obtaining thermographic data of the body part, (3) obtaining range of motion data for the body part, and (4) obtaining spectral data of light reflected from the body part. The two or more types of data are integrated to allow them to be interacted with simultaneously, and the body
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Synthetic Osteochondral Composite and Method of Fabrication Thereof

An osteochondral composite (10) including a hydrogel (24) with implanted biological cells (26) joined to a porous supportive base (12) at a mechanically-interlocked interface is disclosed. A method of fabricating the osteochondral composite (10) and a method of treating damaged or diseased articular cartilage by implanting an osteochondral composite (10) are also disclosed.
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Treatment of Type 1 Diabetes Before and After Expression of Predisposition Markers

The present invention relates generally to the use of compounds, compositions, combinations, kits, and methods for the effective endocytic presentation of immunosuppressive factors for the down regulation of diabetogenic T cells for the treatment of type 1 diabetes.
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Rotator Cuff Bone-tendon Allograft

The invention provides a biomaterial and a method of selecting a biomaterial for a rotator cuff repair procedure. The biomaterial includes a bone-tendon allograft and the use of the biomaterial in rotator cuff repairs may provide immediate bone-tendon integrity and function, which may result in lower failure rates and enhanced clinical success.
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Calcium Phosphate Bodies and a Process for Making Calcium Phosphate Bodies

Among the various aspects of the present invention is a process for making calcium phosphate bodies comprising amorphous calcium phosphate, hydroxyapatite or calcium triphosphate, the bodies themselves and the use of such bodies in any of a variety of applications.
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Bacillus Based Delivery System and Methods of Use

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.
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High-throughput and Non-invasive Method to Vitrify Porcine Embryos

The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the IVP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos a
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Methods for Ameliorating Adverse Effects Associated with Transfusion of Aged Red Blood Cells

The present invention provides, inter alia, methods for ameliorating an adverse effect in a patient caused by an acute transfusion into the patient of a composition containing aged red blood cells using an iron chelator. Apparatuses and kits for ameliorating such adverse effects are also provided.
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Modulation of Phospholipase D for the Treatment of Neurodegenerative Disorders

The present invention relates to methods of treating neurodegenerative diseases comprising administering, to a subject in need of such treatment, one or more agent that inhibits or reduces the action, including the catalytic activity, of an enzyme of the phospholipase D family, such as phospholipase D1 and/or phospholipase D2. The present invention also relates to cell-based assays which may be used to identify agents that inhibit or reduce the activity of enzymes of the phospholipase D family a
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Antimicrobial Compositions Containing Low Concentrations of Botanicals

The present invention relates to a preservative or antimicrobial compositions with broad spectrum antimicrobial activity comprising low concentrations of essential oil (and/or one or more component thereof) and a botanical extract in synergistic combination with a fruit acid and alkanediol, and optionally a solvent. The compositions of the invention may be used in personal care products such as creams or soap products.
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Polyamine Inhibitors for the Treatment and Prevention of Parkinson's Disease

Disclosed herein are methods for treating a disease involving a-synucleic aggregation using (1) a compound which reduces the amount of polyamines in an amount effective to reduce a-synucleic aggregation; (2) a compound which inhibits polyamine synthesis in an amount effective to reduce a-synucleic aggregation; or (3) a compound which inhibits a- synucleic aggregation in an amount effective to reduce a-synucleic aggregation. Also disclosed are methods for reducing the amount of a-synucleic aggreg
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Shh Regulation and Methods Thereof

The invention provides for methods of upregulating endogenous GDNF by inhibiting Shh signaling. The invention further provides a method for increasing the production of cholinergic neurons and dopamine neurons by subventricular zone (SVZ) neurogenesis in a subject. The invention further provides methods for treating a neurodegenerative disorder in a subject.
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Treatment of Diseases with Altered Smooth Muscle Contractility

The present invention provides, inter alia, methods and compositions for treating or ameliorating the effects of a disease characterized by altered smooth muscle contractility, such as e.g., asthma.
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Synergistic Transcription Modules and Uses Thereof

The invention provides for methods for treating nervous system cancers in a subject. The invention further provides methods for treating nervous system tumor cell invasion, migration, proliferation, and angiogenesis associated with nervous system tumors.
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Methods of Diagnosing, Preventing and Treating Bone Mass Diseases

The present invention provides methods and therapeutic agents for lowering or increasing serum serotonin levels in a patient in order to increase or decrease bone mass, respectively. In preferred embodiments, the patient is known to have, or to be at risk for, a low bone mass disease such as osteoporosis and the agents are TPHl inhibitors or serotonin receptor antagonists.
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Compositions and Methods of Treating Chronic Pain By Administering Propofol Derivatives

Compositions and methods for managing or treating chronic pain are provided. More particularly, methods are provided for managing or treating chronic pain by administering to a patient in need thereof an effective amount of propofol or a propofol derivative having limited anesthetic properties. Methods of modulating HCN channel gating are also provided. Pharmaceutically acceptable compositions for, e.g., modulating HCN channel gating are further provided.
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Regeneration of endogenous myocardial tissue by induction of neovascularization

This invention provides a method of treating a disorder of a subject's heart involving loss of cardiomyocytes which comprises administering to the subject an amount of an agent effective to cause cardiomyocyte proliferation within the subject's heart so as to thereby treat the disorder. This invention further provides the instant method wherein the agent is human endothelial progenitor cells, G-CSF, GM-CSF, SDF-1, and IL-8. This invention also provides methods of determining the susceptibility o
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Coronary Sinus Cannula with Left Ventricle Lead and Pressure Tent

Techniques for biventricular pacing include a rigid shaped stylet approximating curves of a coronary sinus and branch vein. Some techniques include a parasternal coronary sinus cannula comprising an outer sheath and an obturator. The obturator is removeably disposed inside the outer sheath from a device end of the hollow shaft. The obturator includes a flexible stem that fits snugly inside the hollow shaft, a malleable core disposed inside the flexible stem, a tapered tip that extends beyond a c
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Methods for Assessing the Immune System in a Patient

Methods of determining the onset or susceptibility of an immunological disease are provided herein. Also provided are immunoassay techniques for carrying out such methods.
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Compositions and Methods for Wound Treatment

Provided herein are compositions, methods, systems, and kits for wound healing. As shown herein, CCN2/CTGF stimulated mesenchymal progenitor cells can form aSMA- fibroblasts. Further, TGF was shown to stimulate further differentiation of aSMA- fibroblasts to myofibroblasts associated with fibrosis. One aspect provides a composition including CCN2/CTGF and a TGF inhibitor, a P38 inhibitor, or a tyrosine kinase inhibitor. Another aspect provides a method of treating tissue wounds with CCN2/CTGF-co
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Treatment of Ophthalmic Conditions with Fluorenone Derivatives

Provided are compositions and methods for treatment of ophthalmic conditions, such as retinal detachment and age-related macular degeneration. Various fluorenone derivatives described herein can stimulate fluid removal from the subretinal space and down-regulate reactive gliosis. Administration of compounds described herein can provide an alternative or an adjunct to an invasive procedure to reattach the retina.
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Methods and Compositions for Treating T-cell Leukemia

The present invention relates to compositions and methods that may be used to diagnose and treat cancer, particularly T-cell leukemia. According to one preferred embodiment of the present invention, methods are provided for determining whether reducing or blocking NOTCH-1 activation will be effective to treat, prevent, or ameliorate the effects of a cancer in a patient, including T-cell leukemia, myeloleukemia, neuroblastoma, breast cancer, and ovarian cancer. The methods generally include deter
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Compounds and Methods for Treating and Preventing Exercise-induced Cardiac Arrhythmias

The present invention provides a method for limiting or preventing a decrease in the level of RyR2-bound FKBP12.6 in a subject, a method for treating or preventing exercise-induced cardiac arrhythmia in a subject, and a method for preventing exerciseinduced sudden cardiac death in a subject. Also provided are uses of JTV-519 in these methods. The present invention further provides methods for identifying agents for use in preventing exercise-induced sudden cardiac death, as well as agents identi
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Compounds and Methods for Inhibiting Serotonin Synthesis

Compounds having the structures below that are TPH1 inhibitors are provided: The compounds are useful for lowering serum serotonin levels in a patient in need thereof, e.g., to increase bone mass. In preferred embodiments, the patient is known to have, or to be at risk for, a low bone mass disease such as osteoporosis.
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Rage Regulates Rock Activity in Cardiovascular Disease

A method is provided for treating a RAGE-related disorder in a subject afflicted therewith comprising administering to the subject a therapeutically effective amount of an antagonist of rho-associated protein kinase 1 (ROCK1) so as to thereby treat the RAGE-related disorder. A method is also provided for treating a ROCK1-related disorder in a subject afflicted therewith comprising adminxstering to the subject a therapeutically effective amount of antagonist of receptor for advanced glycation end
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Turkey Viral Hepatitis Virus and Uses Thereof

The invention is directed to a isolated a Turkey Viral Hepatitis picornavirus-like viruses associated with Turkey viral hepatitis, and isolated nucleic acids sequences and polypeptides thereof. The invention also relates to antibodies against antigens from Turkey Viral Hepatitis picornavirus-like viruses. The invention also relates to iRNAs which target nucleic acid sequences of the Turkey Viral Hepatitis picornavirus-like virus. The invention is related to methods for detecting the presence or
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Use of a melanoma differentiation associated gene (mda 7) for reversing a cancerous phenotype

This invention provides a method for reversing the cancerous phenotype of a cancer cell by introducing a nucleic acid having the melanoma differentiation associated gene (mda-7) into the cell under conditions that permit the expression of the gene so as to thereby reverse the cancerous phenotype of the cell. This invention also provides a method for reversing the cancerous phenotype of a cancer cell by introducing the gene product of the above-described gene into the cancerous cell so as to ther
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Ngal-binding Siderophores and Use Thereof to Treat Iron Deficiency and Iron Overload

The invention provides compositions comprising a lipocalin, such as NGAL, and a mammalian siderophore that are useful as iron chelators and iron donors. The invention also provides mammalian siderophore compounds of Formula (I): The invention further provides, methods of treatment and methods of diagnosis.
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Human Notch3 Based Fusion Proteins As Decoy Inhibitors of Notch3 Signaling

This invention provides a fusion protein comprising a signal peptide, EGF repeats 1-X of the extracellular domain of human Notch3 receptor protein wherein X is any integer from 12 to 34, and an Fc portion of an antibody bound thereto. This invention also provides a method for treating a subject having a tumor, a method for inhibiting angiogenesis in a subject, a method for treating a subject having ovarian cancer, and a method for treating a subject having a metabolic disorder, comprising admini
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Di- und Triphosphat-Prodrugs, insbesondere Nukleosiddi- und -Triphosphat-Prodrugs

The invention relates to a propharmaceuticals useful compounds, pharmaceutical compositions produced therefrom, and drug and pharmaceutical dosage forms and a process for preparing the compounds of the invention. The invention provides bioreversible masked nucleoside diphosphates (NDP) and triphosphates (NTP) and related nucleoside analogues, which are particularly suitable for treating viral diseases such as AIDS.
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Novel ansamitocin derivatives

This invention relates to the field of biologically active compounds and specifically to novel ansamitocin derivatives, pharmaceutical compositions comprising these novel ansamitocin derivatives, methods for the production of the ansamitocin derivatives of the general formula (I): and their use for the treatment of cancer.
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Novel Ansamitocin Derivatives

Provided are among other things ansamitocin derivatives, pharmaceutical compositions comprising these novel ansamitocin derivatives, methods for the production of the ansamitocin derivatives and their use for the treatment of cancer.
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Treatment and Prophylaxis of Epilepsy and Febrile Seizures

Provided are methods for treatment and prophylaxis of convulsive disorders and seizures, such as epilepsy and febrile seizures, by modulating TRPVl channel activation.
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Diagnosing, Prognosing, and Early Detection of Cancers By Dna Methylation Profiling

A method of employing DNA methylation analysis for the diagnosis, prognosis, and prediction of cancer.
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Compositions and Methods for Cardiac Tissue Repair

The invention features compositions comprising agents having cardiac protective activity isolated from epicardial progenitor cells and derivatives thereof, and methods for the use of such compositions.
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Compositions and Methods for Tissue Repair

The invention features compositions comprising mesenchymal stem cells or multipotent stromal cells, agents secreted by such cells in culture, and methods featuring such cells for the repair or regeneration of a damaged tissue or organ. The present invention is based, at least in part, on the discovery that agents secreted by bone marrow mesenchymal stem cells or multipotent stromal cells (MSCs) were useful for the treatment or prevention of tissue damage related to ischemic injury (e.g., cerebra
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Ribonuclease and Thiazolidinedione Compounds and their Use in Methods to Treat Cancer

The present invention relates to methods and kits for the treatment of cancer. A novel drug combination comprising a ribonuclease compound and a thiazolidinedione compound has been identified as producing a synergistic cytotoxicity effect in cancer cells. Methods and kits pertaining to the co-administration of these compounds are discussed herein.
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Ccl18 and Ccl3 Methods and Compositions for Detecting and Treating Cancer

The invention relates to methods of treating cancer by administering one or more chemokines that are downregulated in cancerous cells. More specifically, the invention provides methods for treating or preventing cancers such as malignant melanoma by administering a chemokine such as CCL18 and/or CCL3. The invention further provides methods for diagnosing cancer such as melanoma.
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Prosthetic Lung

A prosthetic lung for receipt by a lung space of a patient includes a mass exchange apparatus for use in blood/air mass exchange, an air sac and an air vessel. The mass exchange includes plural blood flow conduits for defining blood flow and a plural air flow conduits for defining air flow. The plural air flow conduits and the plural blood flow conduits at least partially include gas-permeable membrane material and the conduits are arranged relative to each other to enable transfer of oxygen fro
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Androgen Receptor Related Methods for Treating Bladder Cancer

Disclosed are compositions and methods for treating bladder cancer.
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Methods for the Treatment Or Prevention of Hemorrhagic Conditions

The present invention relates to a method for treatment or prevention of a hemorrhagic condition in a patient by administering plasminogen activator inhibitor-1 ("PAI-1") and/or an inhibitor of deubiquitinating enzyme CYLD ("CYLD") to a patient. Pharmaceutical compositions that include one or both of PAI-1 and an inhibitor of CYLD are also disclosed.
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Protecting and Repairing Cartilage and Musculoskeletal Soft Tissues

Provided herein are compositions and methods related to promoting protection or repair of articular cartilage and/or musculoskeletal soft tissue by contacting the cartilage, tissues or cellular components thereof with a parathyroid hormone/parathyroid hormone-related protein (PTH/PTHrP) receptor agonist or releasing factor.
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Targeting of Histone Deacetylase 2 and Protein Kinase Ck2 for Treatment of Inflammatory Diseases

Methods for the treatment or prevention of diseases which are caused by the degradation of histone deacetylase 2 (HDAC2) in cells are described. The diseases which may be treated by the methods of the invention include chronic obstructive pulmonary disease (COPD) and asthma. The invention provides methods for treating or preventing of diseases caused by the degradation of HDAC2 by providing to the subject in need of treatment or prevention a molecular compound capable of preventing the degradati
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Methods and Compositions Related to Aglycosidic Esculentoside A

Disclosed are compositions related to water soluble selective COX-2 inhibitors and methods of using the inhibitors (including h-EsA and derivatives thereof.)
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Methods and Compositions for the Treatment Or Prevention of Pathological Cardiac Remodeling and Heart Failure

The invention relates to methods of treating or preventing pathological cardiac remodeling and/or preventing heart failure. These methods include the administration of a PDEl inhibitor to a patient under conditions effective to treat or prevent pathological cardiac remodeling, and therefore heart failure that occurs as a result of such remodeling. Pharmaceutical compositions and delivery vehicles that can be used in the methods of the present invention are also disclosed herein.
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Methods and Compositions for Treating Inflammatory Conditions

The present invention is directed to methods of treating an inflammatory condition in a patient. This method includes providing a therapeutic agent that is a vascular endothelial growth factor receptor-3 (VEGFR-3) agonist or a nucleic acid molecule encoding a VEGFR-3 agonist. The present invention is further directed to pharmaceutical compositions and therapeutic systems for treating an inflammatory condition.
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Molecular Targets for Treatment of Inflammation

Molecular targets and methods for treating inflammatory disorders are described. The expression and/or functionality of molecular targets RelB or SIRT1 is modified in order to treat inflammatory disorders. The expression of RelB or SIRT1 may be increased, or the activity of RelB or SIRT1 may be increased in order to inhibit transcription factors which activate genes involved in inflammation. Inflammatory disorders such as chronic obstructive pulmonary disorder, rheumatoid arthritis, asthma and i
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Use of Electrophilic Compounds for Inducing Platelet Production Or Maintaining Platelet Function

The present invention is directed to a method of inducing platelet production that includes contacting a megakaryocyte with an electrophilic compound under conditions effective to induce platelet production by the contacting megakaryocyte. Methods of treating a patient for low platelet levels, increasing the circulating half- life of platelets, and improving the quality (activity) of platelets are also disclosed herein, which involve administering the electrophilic compound to a patient an effec
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Parathyroid Hormone Treatment for Enhanced Allograft and Tissue-engineered Reconstruction of Bone Defects

Methods of improving an outcome of a bone allograft procedure in a subject suffering from a massive bone defect are described, including providing a bone allograft to the subject and intermittently providing said subject with parathyroid hormone (PTH); where the PTH is provided in an amount effective to enhance in or adjacent to a bone allograft, relative to a patient not provided the PTH, at least one of callus bone volume, callus mineral content, callus bridging, graft stiffness, graft incorpo
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Methods and Compositions Related to Tr4 Ligand

Disclosed are compositions and methods related to TR4 and aging.
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Use of Biliverdin Reductase (bvr) and Bvr Peptide Fragments to Treat Coronary Disorders

The present invention relates to methods and compositions for treating coronary disorders and preventing cardiac cell death that involve the use of biliverdin reductase ("BVR") or BVR peptides to stabilize the expression and activity of intracellular HO-2 in cardiac cells. The present invention also relates to methods and compositions for inducing cancer cell death in a patient having cancer that involve the use of inhibitory BVR peptides to destabilize intracellular HO-2 expression and activity
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Methods of Modifying P53 Acetylation and Treating Cancer Using Avra

The present invention relates to methods and compositions for the treatment of cancer. The methods and compositions involve the use of the Salmonella effector protein AvrA, as well as variants or fragments thereof, or encoding nucleic acids. The AvrA effector protein is demonstrated to enhance p53 acetylation, disrupt the cell cycle progression in treated cells, and enhance the killing of cancer cells. In this way, the methods and compositions can treat cancerous conditions either alone or in co
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Aberration Control By Corneal Collagen Crosslinking Combined with Beam-shaping Technique

Corneal collagen crosslinking is used to alter a characteristic of the cornea, such as thickness or refractive index, to correct wavefront aberrations, including higher-order aberrations. A scanning laser or the like is used to perform the corneal collagen crosslinking by locally altering the optical path length (thickness, refractive index, or both).
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Methods of Diagnosing and Treating Fibrosis

The present invention is directed to methods of diagnosing and treating a fibrotic condition in a mammalian subject. These methods involve measuring the levels of trimethylation at lysine residue 27 of histone-3 and/or measuring the expression levels of EZH2 or YY-1. Agents useful for treating fibrosis or a fibrotic condition are also disclosed.
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Compositions that inhibit proliferation of cancer cells

The present invention relates to compositions and methods for reducing the proliferation of cancer cells through targeted interactions with integrin.
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Fibroblast Growth Factor (fgf) Analogs and Uses Thereof

The invention relates to novel peptide comprising FGF-P and methods of use thereof.
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Method for Modifying the Refractive Index of Ocular Tissues

A method for providing vision correction to a patient. The method includes: (a) measuring the degree of vision correction needed by the patient and determining the location and shape of refractive structures that need to be positioned within the cornea to partially correct a patient's vision; (b) directing and focusing femtosecond laser pulses in the blue spectral region within the cornea at an intensity high enough to change the refractive index of the cornea within a focal region, but not high
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Methods of Treating Inflammatory Intestinal Disease and Managing Symptoms Thereof

Methods and products are disclosed for treating an inflammatory intestinal disease in a mammalian subject in need thereof, or preventing or reducing a symptom of inflammatory intestinal disease. These method include administering to the subject a therapeutically effective dose of (i) an isolated AvrA protein or polypeptide fragment thereof or (ii) a nucleic acid molecule encoding the isolated AvrA protein or polypeptide fragment. Preferred inflammatory intestinal diseases include Inflammatory Bo
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Method of Generating ?0 Cells

Described is a method of the generation of ? DEG cells using a mitochondrial targeted restriction endonuclease. This method comprises (a) transfecting cells with an expression vector containing a gene encoding a fusion protein comprising a mitochondrial targeting sequence (MTS) and a restriction endonuclease operatively linked to a suitable promoter; (b) culturing the transfected cells over a sufficient period of time; and (c) selecting ? DEG cells, e.g., via FACS analysis.
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Method and Kit for the Ex Vivo Evaluation of the Response of a Tumor to Conditions to Be Tested

The invention concerns a method and kit for the ex vivo evaluation of the response of a tumor to conditions to be tested, in particular a tumor treatment regime, in an isolated tumor sample. The diagnostic method is suitable for the use in medicine and in pharmacy, in particular for the pretherapeutical evaluation of the responsiveness of a tumor to a tumor treatment regime and the preclinical evaluation of a tumor treatment regime. The method comprises: a.) Placing a freshly isolated tumor samp
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Cancer Treatment with Gama-secretase Inhibitors

Provided are methods for treating cancer in a patient, comprising administering to a patient in need thereof a therapeutically effective regimen, the regimen comprising administering a gamma-secretase inhibitor, wherein the regimen results in a reduction in the cancer cell population in the patient. In some embodiments of the methods, the therapeutically effective regimen stabilizes, reduces or eliminates the cancer stem cell population. Also provided are compounds of the formula I or a pharmace
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Immunotherapy for Contact Dermatitis Using Co-signal Regulation

Compounds, compositions and methods for immunotherapy in contact dermatitis. Compounds and compositions (e.g. monoclonal antibodies) that stimulate the LAIR-1 expression pathway in LAIR-1 expressing immune cells and are useful for preventing or treating contact dermatitis are provided.
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Inhibitors of Hedgehog Signaling Pathways, Compositions and Uses Related Thereto

The present invention makes availables assays and reagents inhibiting paracrine and/or autocrine signals produced by a hedgehog protein or aberrant activation of a hedgehog signal transduction pathway, e.g., which involve the use of a steroidal alkaloid or other small molecule.
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Compositions and Methods for Treating Hepatic Neoplasia

The present invention provides compositions and methods featuring miR-26 microRNA polynucleotides for the diagnosis, treatment or prevention of hepatic neoplasia.
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Peroral transgastric endoscopic techniques

Methods and apparatus for accessing the peritoneal cavity and for ligation of fallopian tubes. A representative method includes using an endoscope to orally access a gastric wall. The gastric wall is punctured to provide access to a peritoneal cavity. The endoscope is advanced into the peritoneal cavity through the puncture. A fallopian tube is located and ligated. The endoscope is removed.
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Methods of Promoting Muscle Growth

The present invention relates to use of the interaction of myostatin with the activin type II receptors, and more specifically to use of soluble ACVR2 to increase muscle growth in a subject. As such, methods of increasing muscle mass in a subject are provided. In addition, methods of ameliorating the severity of a pathologic condition characterized, at least in part, by a decreased amount, development or metabolic activity of muscle are provided.
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Compositions and Methods for Enhancing Antigen-specific Immune Responses

Methods for treating or preventing recurrence of hyper proliferating diseases, e.g., cancer, are described. A method may comprise priming a mammal by administering to the mammal an effective amount of a nucleic acid composition encoding an antigen or a biologically active homo log thereof and boosting the mammal by administering to the mammal an effective amount of an oncolytic virus comprising a nucleic acid encoding the antigen or the biologically active homolog thereof.
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Compositions and Methods for Inactivation of Pathogens At Genital Tract Surfaces

The present disclosure includes compositions and methods of inactivating pathogens of a genital tract of a female. The compositions include L-lactic acid substantially free of D-lactic acid. In particular, an intravaginal ring for sustained release of L-lactic acid out of a reservoir containing multi-gram quantities of L-lactic acid, with minimal osmotically induced swelling or pressurization of the reservoir during prolonged use is provided.
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Target for Therapy of Cognitive Impairment

PROBLEM TO BE SOLVED: To provide a method of identifying a gene associated with a desired behavior in a mammal. ; SOLUTION: The method of identifying a gene associated with the desired behavior in a mammal comprises the steps of: (a) providing a test population of mammals having the desired behavior; (b) providing a control population of mammals lacking the desired behavior; (c) isolating and pooling expressed RNA from neural tissue of the test population; (d) isolating and pooling expressed RNA
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Methods for Protecting the Skin from Radiation Insults

The present invention relates to methods and compositions for the protection of skin and mucous membranes from undesirable side effects of ionizing radiation in a patient undergoing ionizing radiation therapy. In particular, the application describes compositions and methods comprising the topical use of Nrf2 inducers.
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Cancer Immunotherapy with a Viral Antigen-defined, Immunomodulator-secreting Cell Vaccine

A human cell line, which lacks major histocompatibility class I (MHC-I) antigens and major histocompatibility class II (MHC-II) antigens and which has been modified to comprise and express (i) a nucleotide sequence encoding an immunomodulator and (ii) a nucleotide sequence encoding a viral antigen, and a method of inducing or stimulating an immune response in a human to a viral-associated disease or cancer comprising administering to the human (i) the aforementioned human cell line in an amount
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Photoinitiated Tissue Filler

Visible light-activated polymer cosmetic filler preparations useful in a variety of applications are provided. In some embodiments, the photo-activated polymer composition comprises a conventional polymeric material, such as HA, together with a modified, cross-linkable polymer, such as PEG or PEODA, to permit the formation of crosslinks within the polymer matrix in situ on exposure to a visible light source, such as an IPL device. The preparations provide for a more stabilized composition that i
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Gold (iii) Complexes with Oligopeptides Functionalized with Sulfur Donors and Use Thereof As Antitumor Agents

The invention concerns Au(III) complexes of the type [AuIIIX2(Pdtc)] (X = halogen, pseudo-halogen; pdtc = peptide-/esterified peptidedithiocarbamato) which are able to both maintain the antitumor properties and the lack of nephrotoxic side-effects of the previously reported Au(lll)-dithiocarbamato complexes, together with an improved bioavailability through the peptide-mediated cellular internalization. The Au(III) complexes described have shown a significant biological activity on human tumor c
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Methods of Treating Cancer with Cd11b Antibodies

Methods and compositions for treating cancer with CD11b antibodies are disclosed. The antibodies may be WT.5 antibodies or compete with WT.5 antibodies, and induce apoptosis in SKOV, MDAH-2774, or BxPC-3 cells
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Erythrocyte Atp-release Modulators

Erythrocyte ATP-release modulators and composition and methods for their use to potentiate serum glucose clearance and vasodilation; methods for prophylactic or palliative therapy of glucose processing or vascular disorders; processes for preparing the modulators and compositions comprising them; and kits therefor.
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Monophosphorylated Lipid a Derivatives

The present invention provides for monophosphorylated lipid A derivatives and carbohydrate derivatives that are useful as agents in the treatment of diseases and conditions, including cancers. Also provided are pharmaceutical compositions comprising one or more compounds of Formula I-IV. In addition, methods for the treatment of cancers are provided.
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Light Therapy Treatment

An exemplary method includes selecting at least one light source configured to generate light at a particular wavelength and applying the light to tissue following an ischemic event. Applying the light to the tissue inhibits cytochrome c oxidase activity. Another exemplary method includes selecting at least one light source configured to generate light at a particular wavelength and applying the light to tissue following an ischemic event and prior to either reoxygenation of the tissue or clinic
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Medicament for Treating Problems Relating to Fertility and Pregnancy, and Autoimmune Diseases, and for Inducing an Immunological Tolerance in Transplant Patients, and Method for Producing Said Medicam

A medicament for treating pregnancy disorders or for inducing an immunological tolerance in patients with autoimmune diseases or transplantation processes, contains at least one each of a) a precursor hCG beta subunit of the human choriongonadotropine (hCG) selected from hCG beta6 according to SEQ ID NO 1 or SEQ ID NO 2 and hCG beta7 according to SEQ ID NO 5 or a mature hCG beta subunit selected from hCG beta6 according to SEQ ID NO 3 or SEQ ID NO 4 and hCG beta7 according to SEQ ID NO 6 or glyc
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Cell culture medium and method for culturing stem cells and progenitor cells

The invention relates to a stem cell culture medium that is deficient in both glucose and inositol. As expected most cells die rapidly as in media deficient in both glucose and inositol. However, in particular somatic stem cells and germ line stem cells are surprisingly capable of survival and growth under these conditions, while maintaining their regenerative potential Thus, the cell culture medium according to the invention allows not only culturing and amplifying but also purification and enr
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Use of the Opposite Cell Differentiation Program (ocdp) for the Treatment of Degenerated Organs in the Pathological State

The present invention relates to a method for the treatment of organs which are degenerative and /or in the pathological state by means of the use of cells, which are phenotypically stably differentiating or differentiated but not necessarily ultimately predetermined with respect to development, from a donor organ selected according to the principles of the opposite cell differentiation program (OCDP) and also to the use of cells of this type for the treatment or for the production of a drug for
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Pharmaceutical Agent for the Treatment of Fertility and Gestation Disorders, Immunological Diseases, and Transplantation for Use in Veterinary Medicine, Particularly in Horses, and Methods for the Pro

The invention relates to pharmaceutical agents for the treatment of fertility and pregnancy disorders, and immunological diseases (autoimmune diseases) and transplantation, and a method for the production thereof for use in veterinary medicine, particularly in horses. According to the invention, a protein constituent of equine chorionic gonadotropin (eCG) or a nucleic acid sequence (ecg) coding for a protein constituent of equine chorionic gonadotropin is utilized for the treatment or diagnosis
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Aurora Kinase Inhibitors Compounds.

The present invention relates to novel compounds of formula (I) that inhibit cell proliferation, in particular by inhibiting selectively Aurora A kinase activity and to their use in medicine, in particular for treating, ameliorating or preventing hyperproliferative diseases. The invention further relate to methods of their preparation, pharmaceutical compositions including such compounds.
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New Rac1 Inhibitors As Potential Pharmacological Agents for Heart Failure Treatment

The present invention finds application in the field of medicine and, in particular, to new compounds particularly active in inhibiting Rac1 member of the Rho family. Pharmaceutical preparations containing them useful for the treatment and/or prevention of heart failure are disclosed as well.
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Treatment and Diagnostic Methods for Fibrosis Related Disorders

The present invention relates to the ability of SAP to suppress fibrocytes. It also relates to the ability of IL-12, laminin-1, cross-linked IgG and IgG aggregates to suppress fibrocytes. Methods and compositions for suppressing fibrocytes using these proteins are provided. These methods are useful in a variety of applications including treatment and prevention of fibrosing diseases such as scleroderma, pulmonary fibrosis and asthma. Finally, the invention includes assays for detecting the abili
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Water-soluble Carbon Nanotube Compositions for Drug Delivery and Medicinal Applications

Compositions comprising a plurality of functionalized carbon nanotubes and at least one type of payload molecule are provided herein. The compositions are soluble in water and PBS in some embodiments. In certain embodiments, the payload molecules are insoluble in water. Methods are described for making the compositions and administering the compositions. An extended release formulation for paclitaxel utilizing functionalized carbon nanotubes is also described.
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Water-soluble Carbon Nanotube Compositions for Drug Delivery and Medical Applications

Compositions comprising a plurality of functionalized carbon nanotubes and at least one type of payload molecule are provided herein. The compositions are soluble in water and PBS in some embodiments. In certain embodiments, the payload molecules are insoluble in water. Methods are described for making the compositions and administering the compositions. An extended release formulation for paclitaxel utilizing functionalized carbon nanotubes is also described.
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System and method for intra-body communication

A method comprising generating a mechanical signal in a mammal, the mechanical signal having a frequency no more than 50,000 Hz, transmitting the mechanical signal through the musculoskeletal system in the mammal, and sensing the mechanical signal from the musculoskeletal system. A method of triggering an internal event comprising generating a mechanical signal internal or external to a mammal, transmitting the signal through the musculoskeletal system of the mammal, detecting the mechanical sig
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Method for Measuring Disease Probability

Methods comprising providing a tissue sample, providing a first imaging device comprising a white light imaging device; providing a second imaging device comprising at least one imaging device selected from the group consisting of: a fluorescent imaging device, a narrowband reflectance imaging device, and a polarized reflectance imaging device; obtaining a first image of the tissue sample with the first imaging device, wherein the first image comprises a plurality of pixels; obtaining a second i
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A Decellularization Method for Scaffoldless Tissue Engineered Articular Cartilage Or Native Cartilage Tissue

Methods for fabricating a tissue-engineered construct comppsing: providing a tissue-engineered construct, wherein the tissue-engineered construct is derived from a xenogenic source; and decellularizing the tissue-engineered construct.
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Scaffoldless Constructs for Tissue Engineering of Articular Cartilage

A process for culturing chondrocytes to form constructs which contain higher percentages of cells that retain the chondrocytic phenotype are disclosed. The tissue engineered constructs may be formed into neocartilage-containing compositions for a variety of<i
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Unique Dual-action Therapeutics

A new family of therapeutics which provides a controlled-release delivery platform for non-steroidal anti-inflammatory agents on an ester or an ester-carbonate backbone is disclosed herein. These agents are reversible inhibitors of acetylcholinesterase and are thus useful for clinical conditions benefiting from inflammation suppression and cholinergic intervention. These compounds are of the general formula wherein n = 0, 1; X = C, Si, and N+ and NSAID = ibuprofen, naproxen, indomethacin and dic
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Treatment of Disorders Associated with G Protein-coupled Receptor 35 (gpr35)

Compounds are provided having agonistic activity against G protein- coupled receptor 35 (GPR35). The compounds are useful for providing antinociception, providing neuroprotection in case of stroke or ischemia, or treating gastric inflammation.
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Leptin Antagonist and Methods of Use

This invention provides compounds that are true antagonists of the leptin receptor, in the presence and the absence of native leptin or another leptin receptor binder. These compounds may be used to inhibit leptin receptor activity, promote growth arrest or death of leptin receptor-positive cancer cells, or monitor or detect a leptin receptor-positive cell, in vitro or in vivo. These compounds may also be used to treat a weight-loss nutritional disorder, osteoporosis, rheumatoid arthritis, osteo
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Angiocidin Inhibition of Tumor Cell Growth

Methods are presented for the therapeutic administration of angiocidin in the treatment of cancers such as glioma, breast cancer, and leukemia. Methods are also presented for inducing growth arrest and/or apoptosis of tumor cells, as well as inducing differentiation of tumor cells to inhibit tumorigenicity and to confer a non- tumor or healthy phenotype.
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Treatment of proliferative disorders with amino-substituted (E)-2,6- dialkoxystyryl 4-substituted benzylsulfones

Methods of treating cancer using compounds according to Formula (I) are disclosed herein, wherein X, X1, X2, Ra, R1, R2, R3, R4, R5, R6, R7, g, M, y, a, b, d, e, V, W, Z and Q are as defined herein.
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Leptin Agonist and Methods of Use

Peptides are provided having leptin receptor agonist activity. The peptides are useful for treating obesity, insulin resistance, lipodystrophy and hypothalamic amenorrhea, anorexia-related infertility, among other diseases and conditions related to leptin deficiency and/or leptin resistance.
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Blood Monocyte Cd163 Expression As a Biomarker in Hiv-1 Infection and Neuroaids

The invention provides a method for detecting CD163+/CD16+ cell population in peripheral blood mononuclear cells in a biological sample from a subject infected with HIV-1 which comprises contacting the biological sample with an anti-CD163 antibody, so that levels of CD163+/CD16+ peripheral blood mononuclear cells in the biological sample can be quantified. The method of the present invention is particularly useful for monitoring the course of HIV-1 infection and/or HIV Encephalopathy
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Engineered Dendritic Cells and Uses for the Treatment of Cancer

This invention provides the field of therapeutics. Most specifically present invention provides methods of generating in vitro engineered dendritic cells conditionally expressing interleukin-12 (IL- 12) under the control of a gene expression modulation system in the presence of activating ligand and uses for therapeutic purposes in animals including human.
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Virus Derived Antimicrobial Peptides

Described herein are peptides having antimicrobial activity (antimicrobial peptides). The antimicrobial peptides, designated LBU, WLBU and WR, are analogs of the Lentivirus Lytic Peptide 1 (LLP1) amino acid sequence. The antimicrobial peptides are monomers or multimers of peptides referred to as the Lytic Base Unit (LBU) peptides, derived from the LLP1 analogs and also having antimicrobial activity. Also decribed herein are using the peptides in a variety of contexts, including the treatment or
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Nitrated-fatty Acids Modulation of Type Ii Diabetes

Nitro oleic acid and related metabolites are agonists of PPAR-?. Surprisingly, nitro oleic acid is a more potent agonist of PPAR-?, relative to nitro linoleic acid. Thus, nitro oleic acid and its metabolites, as well as their pharmaceutically acceptable salts and prodrug forms, are candidate therapeutics for the treatment of type-2 diabetes, which results from insulin resistance accompanying the improper functioning of PPAR-?.
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Methods for Heat Shock Protein Dependent Cancer Treatment

The present invention provides a method of treating an Hsp70 dependent cancer, including: providing at least one Hsp70 dependent cancer cell; contacting the at least one cell with a sub-effective concentration of a dihydropyrimidinone compound; and contacting the at least one cell with a sub-effective concentration of a proteasome inhibitor, wherein the sub-effective concentration of the dihydropyrimidinone compound and the sub-effective concentration of the proteasome inhibitor have a synergist
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Surfactant-based Antimicrobial Solution for Inhalation

A surfactant can be added, safely and effectively, to a drug solution containing any antimicrobial agent, such as an antibiotic like tobramycin, that is suitable for administration to the lungs via inhalation. Thus, when an aerosolized drug solution includes surfactant, Marangoni flows cause the drug particles, once deposited in the lungs, to spread over a wider surface area, thereby ensuring greater antimicrobial efficacy. A solution that contains, for example, an antibiotic and tyloxapol or an
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Radioprotective Agents

Provided herein are methods, for reducing, preventing, mitigating and treating damage caused by radiation. The methods comprise delivering a compound, as described herein, to a patient in an amount and dosage regimen effective to prevent, mitigate or treat damage caused by radiation or to mitigate apoptosis. The compounds comprise glyburide or other sulfonylurea hypoglycemic compounds or potassium channel inhibitors.
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Administration of an Adsorbent Polymer for Treatment of Systemic Inflammation

The invention provides a method of ameliorating systemic inflammation in a patient involving administering to the patient a therapeutically effective dose of composition including polystyrene divinyl benzene copolymer and a polyvinyl pyrrolidone polymer. More particularly, the method relates to using these polymers as an enteral sorbent preparation to remove inflammatory mediators, such as cytokines, from the intestinal lumen. The polymers can be in the form of a preparation of polystyrene divin
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Methods and Compositions for Regulating Th2 and Th17 Responses

Methods are provided for treating diseases, such as asthma, inflammatory bowel disease, autoimmune disease and cancers in which IL-6 production by dendritic cells is elevated. Dendritic cell c-Kit signaling is implicated herein in the production of IL-6 and an elevated TH17 response, such as an elevated TH2/TH17 response. Inhibition of c-Kit activity can reduce the elevated IL-6 production via a pathway that involves stem cell factor (KIT ligand), PI3 kinase p110d and the Notch ligand Jagged-2.
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Lymph Nodes As a Site for Regeneration

Methods of transplanting cells, such as hepatocytes, are presented herein. Such methods are useful for treating liver disease as well as other disorders.
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Vocal Cord Augmentation Utilizing Muscle-Derived Progenitor Compositions, and Treatments Thereof

The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment vocal cord tissue following introduction (e.g. via injection, transplantation, or implantation) into the vocal cords. Also provided are methods of isolating muscle-derived progenitor cells, and methods of genetically modifying the cells for gene transfer therapy. The invention further provides methods of using compositions comprising muscle
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Methods of Treating Disorders Associated with Protein Polymerization

The present invention relates to methods of treatment of clinical disorders associated with protein polymerization comprising administering, to a subject, an effective amount of carbamazepine, oxcarbazepine or another carbamazepine-like compound. It is based, at least in part, on the discovery that, in cells having a genetic defect in a1-antitrypsin, carbamazepine was able to decrease levels of the mutant protein. Furthermore, carbamazepine reduced the hepatic load of mutant a1 -antitrypsin and
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Fatty Acids As Anti-inflammatory Agents

Compounds of formula I and their metabolites are potent mediators of an inflammatory response: (I) where a, b, c, d, e, f, V, W, X, Y, Ra, Ra', Rb, Rb', Rc, and Rc'are defined herein. In particular, the compounds of the invention are candidate therapeutics for treating inflammatory conditions.
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Removal of Oxygen from Biological Fluids

A system for reducing the concentration of oxygen in a fluid including red blood cells includes a housing, a plurality of hollow tubes extending within the housing and adapted for flow of the fluid therethrough, wherein each tube includes an inlet and an outlet, and a carrier system that reduces the concentration of oxygen at an exterior surface of the tubes to facilitate transport of oxygen from the fluid flowing through the tubes to an exterior of the tubes.
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Estrogen Antagonists As Treatments for Sclerosing Disorders

The present invention relates to methods and compositions for treating a subject suffering from a sclerosing disorder, comprising administering, to the subject, an effective amount of an estrogen receptor antagonist ("ERANT"), wherein said ERANT has essentially no estrogen receptor agonist activity under physiologic conditions.
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Use of Targeted Nitroxide Agents in Bone Healing

Provided herein are compositions and related methods useful for accelerating bone healing and growth. The compounds comprise a nitroxide-containing group attached to a mitochondria-targeting group. The compounds can be cross-linked into dimers without loss of activity.
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Blood Vessel Segmentation with Three-dimensional Spectral Domain Optical Coherence Tomography

In the context of the early detection and monitoring of eye diseases, such as glaucoma and diabetic retinopathy, the use of optical coherence tomography presents the difficulty, with respect to blood vessel segmentation, of weak visibility of vessel pattern in the OCT fundus image. To address this problem, a boosting learning approach uses three-dimensional (3D) information to effect automated segmentation of retinal blood vessels. The automated blood vessel segmentation technique described here
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Constitutive Androstane Receptor (car) As a Therapeutic Target for Obesity and Type Two Diabetes

The invention provides a method of controlling obesity or type two diabetes in a human. In accordance with the inventive method, the constitutive androstane receptor (CAR) is agonized within the human, which effectively controls obesity or type two diabetes.
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Anti-hmg Monoclonal Antibodies and Compositions Containing the Same

Compositions and methods are disclosed for inhibiting the release of a proinflammatory cytokine from a vertebrate cell, and for inhibiting an inflammatory cytokine cascade in a patient. The compositions comprise a vertebrate HMG A box, and an antibody preparation that specifically binds to a vertebrate HMG B box. The methods comprise treating a cell or a patient with sufficient amounts of the composition to inhibit the release of the proinflammatory cytokine, or to inhibit the inflammatory cytok
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Stable Lipid-comprising Drug Delivery Complexes and Methods for their Production

Novel stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described. The biological activity of the complexes produced are comparable to the formulations prepared according to the prior art admixture method and upon purification, the complexes produced by the method of this invention are 50 to 500 fold more concentrated than the complexes formed by admixture. The method described herein provides for the large sc
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Urinary Biomarkers for Predicting Long-term Dialysis

This invention is related to the field of the prevention and treatment of kidney disease. The treatment of kidney disease may be tailored depending upon the need for, or expectation of, long-term dialysis. For example, prediction of long-term dialysis treatment can be determined by monitoring urine biomarkers related to the development of chronic kidney disease. For example, a normalized time course of hyaluronic acid can be used to determine whether a patient having suffered acute kidney injury
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Methods of Treating Disorders Associated with Protein Aggregation

The present invention relates to methods of treatment of clinical disorders associated with protein aggregation comprising administering, to a subject, an effective amount of an anti- protein aggregate ("APA") compound selected from the group consisting of pimozide, fluphenazine (e.g., fluphenazine hydrochloride), tamoxifen (e.g., tamoxifen citrate), taxol, cantharidin, cantharidic acid, salts thereof and their structurally related compounds. It is based, at least in part, on the discovery that
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Compositions and Methods for Detecting Cancer

The present invention provides methods and compositions involving detecting the presence of and/or assessing the risk of cancer in a subject. These methods include methods of detecting and diagnosing cancer in an individual; methods of identifying individuals at risk of developing a cancer; and methods of staging a cancer. The methods generally involve detecting a palladin gene nucleotide sequence alteration that has been found to be associated with cancer and/or detecting a level of a palladin
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Use of Endostatin Peptides for the Treatment of Fibrosis

C-terminal endostatin polypeptides are disclosed herein. These polypeptides include or consist of at least at least 40 consecutive amino acids of the amino acid sequence set forth as amino acids 133-180 of SEQ ID NO: 2 or SEQ ID NO: 13 with at most 5 amino acid substitutions, wherein the polypeptide has anti-fibrotic activity and wherein the polypeptide does not comprise amino acids 1-92 of SEQ ID NO: 2 or SEQ ID NO: 13. Polynucleotides encoding these polypeptide, host cells transformed with the
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Matricryptic Ecm Peptides for Tissue Reconstruction

Chemoattractant polypeptide compounds for progenitor cells and compositions and drug products comprising the compounds are provided herein. Methods for attracting progenitor cells to a location in or on a patient also are provided along with methods of growing and repairing bone.
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Devices, Systems and Methods for Cell Modification

A method of modifying cells includes removing fluid including cells from a patient, contacting the removed fluid from the patient with at least one surface upon which at least one agent to interact at least one cell receptor is immobilized to modify cells in the fluid, and returning the fluid to the patient. The agent can, for example, be immobilized via covalent bonding or ionic bonding to the at least one surface. The fluid can, for example, be blood or a blood fraction. The agent can, for exa
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Non-invasive Method to Treat Urological and Gastrointestinal Disorders

Provided herein are methods and devices useful for inhibiting or treating urological conditions, such as overactive bladder (OAB) symptoms including bladder overactivity, urinary frequency, urinary urgency, urinary incontinence, interstitial cystitis (IC), urinary retention, and pelvic pain; gastrointestinal conditions, such as fecal incontinence, irritable bowel syndrome (IBS), and constipation; and sexual conditions, such as premature ejaculation, erectile disorder, and female sexual arousal d
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Method for Treating Alcoholic Hepatitis

Disclosed is a method for treating acute alcoholic hepatitis in a subject. The method comprises the step of administering to the subject an effective amount of an ester of an alpha-ketoalkanoic acid or an amide of an alpha-ketoalkanoic acid.
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Wortmannin Analogs and Method of Using Same

Wortmannin analogs and their use in inhibiting PI-3-kinase activity in mammals and the treatment of cancer and tumour formation in a subject are described herein. The wortmannin analogs have utility in the treatment of cancer, in particular, lung cancer.
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Bone Substitute Compositions, Methods of Preparation and Clinical Applications

The present invention relates to bone substitute compositions and methods of their preparation, and their use in a wide variety of clinical applications. The compositions include calcium phosphate, acidic calcium salt, basic calcium salt, sodium hydrogen phosphate and porogen. The compositions further include a mixing liquid. The compositions can optionally include biological signaling molecules and/or a growth compound. Further, the compositions can optionally include a plasticizer.
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Functional Near-infrared Spectroscopy As a Monitor for Depth of Anesthesia

Disclosed are methods and devices for measuring a state of anesthesia in a noninvasive manner. Optical techniques may be used to measure changes in a functional near-infrared (fNIR) signal, where the fNIR signal is received in response to directing wavelengths of light in a near-infrared range on a patient. The optical density change may be used to obtain a change in deoxyhemoglobin (deoxy-Hb) concentration and/or a change in an oxyhemoglobin concentration (oxy-Hb). The changes in the deoxy-Hb a
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Tissue Joining Device and Instrument for Enabling Use of a Tissue Joining Device

A tissue joining device and a surgical instrument for employing the tissue joining device are provided. The tissue joining device may be designed such that a first member may be inserted into a second member in discrete increments so that two lumen structures can properly be combined.
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Local Delivery System for the Chemotherapeutic Drug Paclitaxel

The present invention provides methods for producing a semi-degradable polymeric composite drug delivery device for localized delivery of chemotherapeutic agents to be used in conjunction with total vertebral body replacement surgery that requires placement of a vertebral replacement cage for the treatment of a spinal neoplasm.
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Dual Inhibitors of HIV-1 GP-120 Interactions

Compounds, which inhibit the binding of gp120 to CD4 as well as 17b and methods for their use in inhibiting the HIV fusion process, are provided.
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Methods and Devices for Treating Surfaces with Surface Plasma

Methods and devices for treating surfaces of objects using a non-thermal plasma are disclosed herein. The non-thermal plasma is generated through the use of an apparatus configured to generate a non-thermal plasma on its surface. The apparatus is comprised of a substrate that contains one or more electrodes of different polarity. The electrodes are placed within a layer of the substrate acting as a dielectric layer. The apparatus may also have additional layers to contain the dielectric layer. W
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Articles and Methods for Repairing Damaged Nervous Tissue

The invention concerns substrates comprising a biocompatible gel and at least one of a plurality of cells, said cells being capable of producing at least one therapeutic agent. Other aspects of the invention concern methods of making such substrates and methods of repairing and regenerating damaged nervous tissue with the substrates.
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Physical Therapy Systems and Methods

The invention is directed to a physical therapy system including a glove with a set of acoustic sensors and a positive feedback system and method for teaching finger extension. The system may also include a pair of gloves with a set of acoustic sensors and a positive feedback system to teach clapping. In another aspect, the system may include a glove with an acoustic sensor, an object with a corresponding sensor and a positive feedback system for teaching arm extension. The invention may be part
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Tricyclic Compounds Having Cytostatic And/or Cytotoxic Activity and Methods of Use Thereof

The present invention provides tricyclic compounds having cytostatic and cytotoxic activity in a single molecule having receptor tyrosine kinase(s), dihydrofolate reductase, thymidylate synthase and/or dihydroorotate dehydrogenase inhibitory activity, which are useful as anti-angiogenic and anti-tumor agents. Also provided are methods of utilizing these inhibitors to treat tumor cells and other proliferative diseases and disorders.
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Methods for treating cancer by inhibiting mitosis using pyrrolo[2,3-d]pyrimidines

A compound having the following formula: wherein X is selected from the group consisting of a C1 to C6 alkyl group, a heteroalkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heteroaryl group, an arylalkyl group, and a heteroarylalkyl group; wherein R 1 is selected from the group consisting of hydrogen, a C1 to C6 alkyl group, a heteroalkyl group, a substituted or unsubstituted aryl group, a substituted or unsubstituted heteroaryl group, an arylalkyl group, an
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Antiretroviral Drug Formulations for Treatment of Children Exposed to Hiv/aids

The present disclosure provides fast disintegrating formulations for the treatment of human immunodeficiency virus (HIV) and acquired immune deficiency syndrome (AIDS) in patients such as neonatal, perinatal and pediatric children. Neonatal and perinatal formulations provide for the prevention or reduction of incidence of mother to child transmission of HIV. Also provided are formulations and methods for treating pediatric children having HIV/AIDS. The orally administered fast disintegrating for
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Cornea Reshaping

A corneal reshaping kit may include a mold defining a concavity that is sized and shaped for receiving an outer corneal surface, such that a corneal surface pressed against the concavity will adopt a convex shape complementary to the concavity, and a biocompatible, flowable material suitable for introduction into a space within a cornea.
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Method and Device for Treating Cardiac Arrhythmias

The present invention provides both methods and devices for termination of arrhythmias, such as ventricular or atrial tachyarrhythmias. The device and method involves application of alternating current (AC) for clinically significant durations at selected therapeutic frequencies through the cardiac tissue to a subject experiencing arrhythmia. Methods are also provided to minimize or eliminate pain during defibrillation.
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Inhibitors of Methionine Aminopeptidases and Methods of Treating Disorders

The invention is directed towards novel naphthoquinone and naphthothiazole compounds, and methods of treating disorders related to MetAP, including tuberculosis and bacterial infection, using various naphthoquinone, hydroxyquinonline, and naphthothiazole compounds.
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Methods for Enhancing Antigen-specific Immune Responses

Methods for delivering naked DNA vaccines to enhance immune responses, by improving transfection efficiency without safety concerns associated with live viral vectors, are described. A method may comprise administering to a mammalian subject an effective amount of a papillomavirus pseudovirion, wherein the papillomavirus pseudovirion comprises at least one papillomavirus capsid protein encapsidating a naked DNA vaccine, wherein the naked DNA vaccine comprises a first nucleic acid encoding at lea
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Truncated Activin Type Ii Receptor and Methods of Use

The present invention provides a substantially purified growth differentiation factor (GDF) receptor, including a GDF-8 (myostatin) receptor, as well as functional peptide portions thereof. In addition, the invention provides a virtual representation of a GDF receptor or a functional peptide portion thereof. The present invention also provides a method of modulating an effect of myostatin on a cell by contacting the cell with an agent that affects myostatin signal transduction in the cell. In ad
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Novel Liver-targeting Agents and their Synthesis

This invention provides novel liver targeting agents and their synthetic methods. A liver targeting agent, with a lysine based nitrilo triacetic acid structure as backbone which acquires multivalency with saccharide groups, to bind with a galactosamine chain or lactose chain is disclosed. In particular, only one amino acid L-lysine is involved to provide trivalency. All carboxyl groups in Ne-benzyloxycarbonyl-Na-dicarboxymethyl-L-lysine can be conjugated with three glycosides of ahGalNAc or ahLa
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Consensus Sequence for Influenza a Virus

Pandemic A(H1N1) continues its global spread, and vaccine production is a serious problem. Protection by current vaccines is limited by the mutational differences that rapidly accumulate in the circulating strains, especially in the virus surface proteins. New vaccine strategies are focusing at conserved regions of the viral internal proteins to produce T cell epitope-based vaccines. T cell responses have been shown to reduce morbidity and promote recovery in mouse models of influenza challenge.
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Reprogramming Blood Cells to Pluripotent and Multipotent Stem Cells

The present invention is based on the seminal discovery that cord blood (CB) and adult bone marrow (BM) CD34+ cells can be reprogrammed to early stem cells. The invention provides the reprogramming of CB and adult bone marrow (BM) CD34+ cells from subjects without any pre-treatment. Provided are methods for reprogramming blood cells of a subject. Also provided are methods of disease modeling and methods of generating subject- specific differentiated cells. In addition, the invention provides met
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Non-invasive Methods and Systems for Assessing Cardiac Filing Pressure

Featured are methods and systems for assessing cardiac filing pressure non- invasively. Such methods include, inter alia, arranging a photoplethysmography (PPG) transducer on a finger of a patient and fluidly coupling a pressure transducer to the patient's mouth so that the pressure transducer measures expiratory pressure. The PPG transducer provides an output of a pulse volume signal of cardiac circulatory flow. Such methods also including determining a pulse amplitude ratio, using the pulse vo
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The Fungal Srebp Pathway As a Target for Anti-fungal Agents

Inhibition of Srel activity and/or expression has great potential for antifungal therapeutics. Disclosed herein are novel methods and compositions useful for the identification of novel inhibitors of Srel activity and/or function and the treatment of fungal infections.
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Lysine-specific Demethylase Inhibitors

Polyamine, polyamine/guanidino, and polyamine/biguanide compounds bearing allene, propargyl, cyclopropyl, and other reactive moieties are disclosed. The compounds are useful as irreversible inhibitors of the enzyme lysine-specific demethylase-1 and for the treatment of cancer.
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Method for Identifying and Validating Dominant T Helper Cell Epitopes Using an HLA-DM-Assisted Class II Binding Assay

Rational design of immunotherapeutics relies on clear knowledge of the immunodominant epitopes of antigens. Current methods for identifying kinetically stable peptide-MHC complexes are in many cases inadequate for a number of reasons. Disclosed herein is a reductionistic system incorporating known participants of MHC class II antigen processing in solution to generate peptide pools from antigens, including those for which no immunodominant epitope has yet been identified, that are highly enriche
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Combination Therapy for Tuberculosis

The present invention relates to methods of treating tuberculosis, including multi not drug resistant varieties and latent tuberculosis. More particularly, the present invention relates to a method of treating tuberculosis in a mammal comprising administering to said mammal in need thereof an effective amount of a compound of formula (I), (S)-N- [[3-[3-fluoro-4-(4-thiomorpholinyl)phenyl]-2-oxo-5-oxazolidinyl]methyl]acetamide, or a pharmaceutically acceptable salt thereof in combination with at l
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Multitype Hpv Peptide Compositions and Methods for Treatment Or Prevention of Human Papillomavirus Infection.

Embodiments of the invention are directed to methods and compositions of multitype HPV polypeptides.
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Methods for Treating Or Preventing Brain Infections

The invention is directed to methods of treating organ specific infections in a host organism by administering compounds that target host receptors and/or host cellular signaling molecules to prevent a pathogen from infecting the organ. For example, the administration of a compound to prevent a pathogen from crossing the blood-brain barrier to prevent a brain infection.
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Universally Deployable and Expandable Bone Screw Anchor Assembly

A self-expanding bone screw anchor assembly adapted to allow the screw body to engage the bone and the anchor to attach thereto. The anchor assembly is adapted to be universally deployed with a variety of installation tools.
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Lrrk2-mediated Neuronal Toxicity

Leucine-rich repeat kinase-2 (LRRK2) mutations are a common cause of Parkinson's disease. Inhibitors of LRRK2 kinase that are protective in in vitro and in vivo models of LRRK2-induced neurodegeneration were identified. The presently disclosed subject matter establishes that LRRK2-induced degeneration of neurons in vivo is kinase dependent and that LRRK2 kinase inhibition provides a potential new neuroprotective paradigm for treating Parkinson's disease.
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Membrane Associated Tumor Endothelium Markers

To gain a better understanding of tumor angiogenesis endothelial cells (ECs) were isolated and gene expression patterns were evaluated. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed differentially expressed genes, including many that were specifically elevated in tum
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Methods of Synthesis and Use of Chemospheres

The present invention provides, in general, compositions comprising a hydrogel and an agent, for example a therapeutic agent or an imaging agent, for locoregional delivery. In certain preferred embodiments of the invention, the hydrogel compositions are detectable by Magnetic Responance and CT Scan and are used for locoregional delivery of therapeutic agents, for example chemotherapeutic agents. The invention also features polymer matrix compositions comprising nanoparticles that can be loaded a
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Histone Deacetylase Inhibitors, Combination Therapies and Methods of Use

The invention relates to histone deacetylase (HDAC) inhibitors to treat proliferative diseases. The present invention provides novel class III histone deacetylase inhibitors, in particular SIRT1 inhibitors, to reverse the silencing of hypermethylated genes, in combination with one or more other agents, in proliferative diseases such as cancer. The present invention provides methods of activating genes that are silenced by methylation in a subject by administering a HDAC inhibitor in combination
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Methods and Compositions for Treating and Preventing Autoimmune Diseases

Provided are methods and compositions for treating and/or preventing an autoimmune diseases, including inflammatory bowel disease, rheumatoid arthritis, diabetes mellitus, celiac disease, autoimmune thyroid disease, autoimmune liver disease, Addison's Disease, Sjögren's Syndrome, transplant rejection, graft vs. host disease, or host vs. graft disease.
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Hydrogel-based Vascular Lineage Cell Growth Media and Uses Thereof

A medium for growing vascular lineage cells is described. The vascular lineage cell growth medium includes an oligosaccharide-based hydrogel and a growth factor that promotes vascularization by vascular lineage cells.
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System and Method for Macro-micro Distal Dexterity Enhancement in Micro-surgery of the Eye

A system for operating within an interior region of the eye, or other organ, includes a delivery channel having a proximal portion located exterior to the eye and a distal portion positionable within the interior region of the eye, wherein the distal portion of the delivery channel defines an outer diameter that is smaller than or equal to about 18 gauge, and a micro-robot extendable from the distal portion of the delivery channel, wherein the micro-robot is remotely operable to change shape wit
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Methods of Treating Neurological Autoimmune Disorders with Cyclophosphamide

escribed herein are methods for treating neurological autoimmune disorders in which the treatment method includes administering an immunoablative agent to eliminate most or essentially all maturing and mature elements of the immune system in an affected individual. Following this step, the individual is administered agents to reestablish the ablated immune system.
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Detection and Prognosis of Lung Cancer

Methods and tools are provided for detecting and predicting lung cancer. The methods and tools are based on epigenetic modification due to methylation of genes in lung cancer or pre-lung cancer. The tools can be assembled into kits or can be used seperately. Genes found to be epigentically silenced in association with lung cancer include ACSL6, ALS2CL, APC2, ART-S1, BEX1, BMP7, BNIP3, CBR3, CD248, CD44, CHD5, DLK1, DPYSL4, DSC2, EDNRB, EPB41L3, EPHB6, ERBB3, FBLN2, FBN2, FOXL2, GNAS, GSTP1, HS3S
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Growth differentiation factor receptors, and methods of using same

The present invention provides an activin type IIB receptor (ActRIIB) for use in the treatment or prevention of a muscle disease or disorder in a mammal. The muscle disease or disorder may be muscular dystrophy, muscle atrophy or a muscle wasting disorder.
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Method for Late Introduction of the (8r)-hydroxyl Group in Carbapenem Beta-lactam Antibiotic Synthesis

The presently disclosed subject matter demonstrates that ThnG and ThnQ enzymes encoded by the thienamycin gene cluster in Streptomyces cattleya oxidize the C-2 and C-6 moieties of carbapenems, respectively. ThnQ stereospecifically hydroxylates PS-5 giving N-acetyl thienamycin. ThnG catalyzes sequential desaturation and sulfoxidation of PS-5, giving PS-7 and its sulfoxide. The ThnG and ThnQ enzymes are relatively substrate selective, but give rise to the oxidative diversity of carbapenems produce
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Treatment Methods Utilizing Stem Cell Mobilizers and Immunosuppressive Agents

The present invention relates to the field of organ transplantation. In one aspect, the present invention provides methods of treating an organ transplant recipient comprising administering to the recipient a therapeutically effective amount of a stem cell mobilizer and an immunosuppressive agent. In particular embodiments, the present invention provides a method of treating an organ transplant recipient comprising administering to the recipient a therapeutically effective amount of an agent tha
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Anti-cytomegalovirus Activity of Artemisinin-derived Dimers

Artemisinin-derived monomers and artemisinin dimers are shown to exhibit in-vitro anti-cytomegalovirus (CMV) activity. Artemisinin dimers effectively inhibited CMV replication in human foreskin fibroblasts and human embryonic lung fibroblasts with no cytotxicity at concentrations required for complete CMV inhibition. Artemisinin dimers were found to be potent and non-cytotoxic inhibitors of CMV replication, which indicates their use as therapeutic agents for the treatment of CMV infection in hum
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Sr-bi As a Predictor of Human Female Infertility and Responsiveness to Treatment

Methods of diagnosis and treatment of diseases and disorders related to de novo synthesis of cholesterol, based on allelic variants of the scavenger receptor class B type I receptor, and kits for use therein.
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Citrullination of Human Peptidylarginine Deiminase 4 (pad-4) Regulates Its Function and Immunogenicity

The present invention provides peptidylarginine deiminase 4 (PAD-4) polypeptides comprising one or more citrullinated Arginine sites. In one embodiment, a PAD-4 polypeptide comprises a citrulline residue at the following sites: Arg-205, Arg-212, Arg-218, Arg-372, Arg-374, Arg-383, Arg-394, Arg-495, Arg-536, and Arg-544. The present invention also provides methods for detecting the presence of autoantibodies to citrullinated PAD-4. The methods of the present invention may also be used to qualify
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Psma-binding Agents and Uses Thereof

Prostate-specific membrane antigen (PSMA) binding compounds having radioisotope substituents are described, as well as chemical precursors thereof. Compounds include pyridine containing compounds, compounds having phenylhydrazine structures, and acylated lysine compounds. The compounds allow ready incorporation of radionuclides for single photon emission computed tomography (SPECT) and positron emission tomography (PET) for imaging, for example, prostate cancer cells and angiogenesis.
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Methods and Compositions for Improving Cognitive Function

This invention relates to treating age-related cognitive impairment. This invention in particular relates to the use of inhibitors of synaptic vesicle protein 2A (SV2A), such as levetiracetam, seletracetam, and brivaracetam, in improving cognitive function in subjects that exhibit age-related cognitive impairment or are at risk thereof, including, without limitation, subjects having or at risk for Mild Cognitive Impairment (MCI), Age-related Cognitive Decline (ARCD) or Age-Associated Memory Impa
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Compositions and Methods for Enhancing Transport Through Mucus

The invention generally relates to compositions and methods for transporting substances across mucosal barriers. The invention also relates to methods of making and using such substances.
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Compositions and Methods for Treating Cardiovascular Conditions

The invention provides amphiphilic macromolecule encapsulates that are useful for treating cardiovascular diseases including conditions related to or emanating from atherosclerosis.
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Nontoxic Shiga-like Toxin Mutant Compositions and Methods

Disclosed are nontoxic mutants of Shiga-like toxin (Stx1 or Stx2), nucleic acids encoding them, compositions containing the mutants and methods of using the mutants in connection with hemolytic euremic syndrome (HUS). Also disclosed are methods of treating HUS using L3 protein fragments, the nontoxic Stx1 or Stx2 mutants, or combinations thereof.
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System and Method for Liver Cell Culture and Maturation

The present invention relates to systems and methods for maturation, proliferation and maintenance of function in cells presenting hepatocyte characteristics and differentiated from stem cells. The cells of the present invention may be generated from stem cell grown in collagen sandwich configuration in the presence of a morphogen (e.g. S-NitrosoAcetylPenicillamine (SNAP) or Oncostatin-M (OSM) ).
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Ampicillin and Amoxicillin-based Poly(anhydride-amides)

In one aspect the invention relates to an antibacterial compound of formula II: wherein n is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12; R is H or OH; or a salt thereof. In other aspects the invention also provides devices that include the compound as well as methods for producing an antibacterial effect with the compound or its salt.
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Endorphin Therapy Compositions and Methods

In certain embodiments, the invention provides methods of isolating and culturing neuronal stem cells from hypothalmi, methods of differentiating the neuronal cells into beta-endorphin neurons, and methods of treatment of various diseases comprising administering agents to differentiate endogenous neuronal stem cells into beta endorphin neurons.
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Hcv E2 Construct Compositions and Methods

The invention provides a construct comprising the ectodomain of the Hepatitis C virus (HCV) E2 sequence and a mammalian expression system therefore. More particularly, the invention relates to a construct comprising the CMV promoter, prolactin signal sequence, the ectodomain of HCV E2 sequence truncated at aa 664, a thrombin cleavage site and the human Fc domain. The invention also relates to an expression system for the construct, which is stably expressed in human embryonic kidney cells 293T.
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Inhibition of Dcps

The disclosure relates to methods and compositions (e.g., compounds and pharmaceutical compositions thereof) useful for increasing expression of SMN in a cell (e.g., in vitro or in vivo). As a deficiency in SMN can result in the development of an SMA condition in a subject, the methods and compositions described herein can also be used to, e.g., treat, or prevent, an SMA condition in a subject.
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Compositions and Methods for Treating Ophthalmic Diseases

Degradable polymeric compositions containing water-insoluble drugs blended with copolymers of biocompatible diphenol compound monomer units with pendant carboxylic acid groups polymerized with biocompatible diphenol corn-pound monomer units with pendant carboxylic acid ester groups and poly(alkylene oxide) blocks, wherein the molar fraction in the copolymer of biocompatible diphenol compound monomer units with pendant carboxylic acid groups and poly(alkylene oxide) blocks relative to the weight
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Controlled Release of Actives in Skin.

Topical compositions are provided in which active compounds for topical delivery through the stratum corneum are complexed with nanospheres of a triblock copolymer having an A-B-A structure wherein each A end block is a water-soluble, hydrophilic and non-toxic polymer or oligomer; and the hydrophobic middle B block is a hydrophobic polymer or oligomer with the same or different repeating units having the structure according to formula:(I) wherein X is - C - R - C - or - C -; Z is between 2 and a
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Compositions and Methods for Modulating Visceral Sensation and/or Gastrointestinal Reflex Activity

The invention relates to compositions capable of modulating visceral sensation and/or gastrointestinal activity obtained from botanical sources. More specifically, the invention relates to compositions comprising an extract product of an Asclepias plant, and methods of modulating visceral sensation and/or gastrointestinal activity in an individual using same.
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Micelle Assemblies

The invention encompasses micelle assemblies, compositions having micelle assemblies, and methods for preparing micelle assemblies and compositions thereof. Also, the invention encompasses compounds of the formula: A-X-Y-Z-R1 wherein A is a carboxy group or is absent; X is a polyol, Y is -C(-O)-, -C(-S)-, or is absent; Z is O, S, or NH; and R1 is a polyether, wherein one or more hydroxy groups of the polyol are acylated with a fatty acid residue, wherein the compounds form micelle assemblies. Th
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Treatment of Obesity

The present invention relates generally to a method of increasing lipid oxidation in a mammal and to agents useful for same. More particularly, the present invention relates to a method of increasing lipid oxidation in a mammal by administering a ligand which interacts with the IL-6 receptor and signals via interaction with a gp130/LIF receptor heterodimer. In a related aspect, the present invention provides a method of increasing insulin sensitivity in a mammal. The method of present invention
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Method for Determining the Quality of Heparin

The invention relates to a method for determining contamination of heparin with other sulfated glycans, comprising the steps: incubating a predetermined amount of heparin together with a predetermined amount of an enzyme that specifically digests heparin for a predetermined duration, wherein the amount of the enzyme corresponds approximately to the amount of the enzyme that would completely degrade an approximately identical amount of contaminant-free heparin within the predetermined duration, o
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Pharmaceutical Compositions for Treating Infections with Drug Resistant Mycobacteria

In a first aspect, the present invention relates to pharmaceutical compositions for the treatment of infections with mycobacteria wherein said mycobacteria are resistant to at least one of the following drugs: rifampicin, isoniazid, pyrazinamide, or ethambutol, in particular, said mycobacteria are Mycobacterium tuberculosis (Mtb) complex strains. That is, the present invention relates to pharmaceutical compositions comprising as an active ingredient hydrazone compounds that have high activity ag
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Neuroprotective Compositions and Methods

This disclosure concerns novel methods for preventing or treating neurological or neurodegenerative diseases or disorders by using kombo butter acid enriched extracts derived from the seeds of African nutmeg, the main active compounds isolated from the extracts, namely sargaquinoic acid, sargachromenol, and sargahydroquinoic acid, or synthetic analogs or derivatives thereof, as neuroprotective agents. The methods for preparing the kombo butter or kombo butter acid extracts and the methods for is
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Therapeutic Devices for Patterned Cell Growth

The invention provides therapeutic devices comprising a polymeric anti-inflammatory agent that biodegrades to release anti-inflammatory agents. The therapeutic devices are useful for repair and regeneration of a variety of injured tissues.
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Process for Designing Inhibitors of Influenza Virus Structural Protein-1

Disclosed are methods and compositions useful in identifying inhibitors of influenza virus, such as influenza A and B virus. Also disclosed are methods for preparing compositions for administration to animals, including humans infected with or to protect against influenza virus.
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Compositions and Methods for Treatment of Neuropathic Pain

The present invention relates to compounds, compositions, methods, systems and kits for treating neuropathic pain regulated by SIP30. The present invention provides SIP30 antagonists for the treatment of neuropathic pain.
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Methods to Treat Cancer

The invention provides methods and pharmaceutical compositions for treating certain cancers with compounds of formula (I) wherein A, B, W, Y, Z, and R1 have any of the meanings defined in the specification and their pharmaceutically acceptable salts and prodrugs.
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Dressing Compositions and Methods

Described is a spray-on hydrogel comprising water-soluble PEG polymers that cross-link in situ to form a hydrogel such that the cross-links are reversible. The hydrogel can be useful as a drug delivery composition, wound dressing or surgery adjuvant. Polyethylene glycol polymer and cross-linker solutions are sprayed simultaneously through a common orifice. Cross-linking via formation of thioether or disulfide bonds is initiated upon mixing, providing rapid gelation. The hydrogel components can b
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Compounds, Compositions and Methods for Treatment of Protozoan Infections

Provided are compounds, compositions and methods for treating protozoan infections.
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Solubilized Topoisomerase Poisons

The invention provides compounds of formula I: wherein A, B, W, Y, Z, and R1 have any of the meanings defined in the specification and their pharmaceutically acceptable salts. The invention also provides pharmaceutical compositions comprising a compound of formula I, processes for preparing compounds of formula I, intermediates useful for preparing compounds of formula I, and therapeutic methods for treating cancer using compounds of formula I.
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Treatment of Cutaneous Hemangioma

This invention concerns a method of treating hemangiomas with a beta blocker by applying the beta blocker onto the hemangiomas directly. The invention also concerns a combination therapy by using a beta blocker along with a corticosteroid or an alpha adrenergic receptor agonist for the treatment of hemangiomas.
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Hiv-1 Gp41 Neutralization Domain and Use Thereof

The invention relates to a region of the HIV-I gp41 protein that contains, at least in part, an epitope that allows potent neutralization of resistant virus particles. This site is present in the C-terminal heptad repeat region of gp41 (HR2), and adjacent to, but distinct from the MPER. Vaccines containing this region as well as monoclonal antibodies which specifically bind to this region and neutralize an HIV-I primary isolate are also provided as is a method for stimulating the formation of an
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Antitoxin Destabilization Technology

Disclosed are methods for purifying toxin proteins which avoid denaturation and renaturation procedures.
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Benzo [c] Phenanthridines As Antimicrobial Agents

The present invention provides compounds of formula I: formula (I) wherein X1- X4 and R1-R12 have any of the values defined in the specification, as well as salts and prodrugs thereof, which inhibit major molecular mechanisms associated with bacterial cell division and proliferation so as to be useful for the treatment and/or prevention of bacterial infections. The invention also provides compositions comprising these compounds as well as methods for using these compounds to inhibit bacterial ce
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Methods and Compositions for Treating Obesity and Related Disorders

The invention provides methods for, and compositions effective for, treating obesity, inhibiting weight gain, treating diabetes mellitus, inhibiting atherosclerosis and treating related disorders and conditions comprising administering a pharmaceutically effective amount of at least one compound capable of inhibiting AC5 to a patient. The compound capable of inhibiting AC5 may be administered singly or in combination with another agent. In some embodiments, the AC5 inhibiting compound is 9-ss-D-
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Bacterial Helicase Inhibitor Compounds and Uses Thereof

Organic compounds are described that inhibit bacterial helicase activity and growth of bacterial cells on surfaces.
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Inhibiting Obesity Progression By Inhibiting Adipocyte Differentiation with a Pre-adipocyte Autophagy Inhibitor

The present invention relates to methods of mitigating, preventing or treating weight gain or obesity in patients by administering one or more autophagy inhibitors, thereby, preventing the differentiation process of pre-adipocyte cells into a mature adipocytes. Specifically, the present invention relates to the surprising discovery that autophagy is critical for the cellular remodeling required during pre-adipocyte differentiation into mature adipocyte. By targeting and inhibiting one or more me
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Methods and Compositions for Treating Alzheimer's Disease

A method is disclosed for inhibiting the build-up of amyloid plaques in the brain of a patient with at least one risk factor for, or a diagnosis of, Alzheimer's Disease by administering to the patient an amount of one or more compounds effective to inhibit the phosphoryla-tive activity of eEF2K, thereby inhibiting amyloid plaque deposition.
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Type 5 Adenylyl Cyclase Inhibitors for Providing Cardiac Protection

The invention provides a method of treating a cardiac disease by administering a pharmaceutically effective amount of at least one compound capable of inhibiting AC5 to a patient. The compound capable of inhibiting AC5 may be administered singly or in combination with another agent, such as, for instance a ss-blocker. In some embodiments, the AC5 inhibiting compound is 9-ss-D-arabinofuranosyladenine (AraAde). The compound may be administered in an amount of about 1 to about 200 mg/kg/day, about
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Amniopunch and Uses Thereof

A tissue, such as an amniotic or chorionic membrane, harvesting device that integrates a system facilitating subsequent storage of tissue samples. The device cuts a sample of the target tissue and automatically deposits the sample in a storage vessel. A method of collecting and storing a sample from a target tissue using a tissue harvesting device. A kit for collecting and storing samples of the target tissue.
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Compositions and Methods to Modulate Cell Membrane Resealing

Disclosed herein are compositions and methods for repairing cell membranes. In addition, the invention relates to therapeutic compositions comprising nucleotides and/or polypeptides of the invention in combination with a pharmaceutically acceptable carrier, wherein the composition facilitates the repair of cell membranes. Moreover, the invention relates to the treatment and/or prevention of pathological conditions associated with cell membrane damage.
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Mechanism-based Small-molecule Parasite Inhibitors

Methods for preventing or treating an Apicomplexan parasite infection in a patient administering to a patient in need thereof an effective amount of a compound of Formulas I- IV.
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Use of Stem Cells for Wound Healing

Cells, compositions, and methods of cell therapy for administering a therapeutically effective amount of stem cells or cell concentrate to achieve accelerated wound healing of normal and chronic wounds, while minimizing the formation of scar tissue.
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Closed System Separation of Adherent Bone Marrow Stem Cells for Regenerative Medicine Applications

A method for isolating and processing bone marrow derived stem cells, including the steps of: (a) collecting a biological sample containing adherent bone marrow stem cells in a receptacle with interior walls coated with a cell-adherent substrate; (b) incubating the bone marrow cells on the adherent substrate so that a layer of adherent bone marrow stem cells adheres to the substrate; (c) washing any non-adherent cells from the substrate; and (d) collecting the bone marrow stem cell layer. Isolat
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Method for Treating Sepsis Or Septic Shock

The present invention is a method for treating sepsis or septic shock using Lipoxin A4.
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Treatment of Hiv Infection

This invention concerns compositions and methods of treating, inhibiting, or controlling HIV infection.
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Treatment and Diagnosis of Inflammatory Disorders

This invention concerns compositions and methods of treating or diagnosing inflammatory disorders and other disorders.
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Method for Treating Chronic Nerve Tissue Injury Using a Cell Therapy Strategy

A method for treating a degenerative or traumatic injury to a nerve tissue or the brain by administering at or near the injury site a composition containing adherent bone marrow stem cells suspended in a pharmaceutically acceptable liquid in an amount effective to elicit axonal regeneration or re-myelination at the site of injury.
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Medical use of mRNA interferase

The present invention is directed to the discovery of a novel family of enzymes designated herein as mRNA interferases that exhibit endoribonuclease activity. The novel finding of the present inventors, therefore, presents new applications for which mRNA interferase nucleic and amino acid sequences, and compositions thereof may be used to advantage. The invention also encompasses screening methods to identify compounds/agents capable of modulating mRNA interferase activity and methods for using
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Hemostatic Effects of Glucono-Delta-Lactone

Glucono-delta-lactone (GDL) can be used to mediate the human blood coagulation process. In particular, GDL can be used in an assay to determine an individual's risk potential for accelerated blood clotting or as a treatment for conditions related to accelerated clotting potential and/or inflammatory states.
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Method of Treatment for Acute Myelogenous Leukemia

This invention relates to a method of treating acute myelogenous leukemia with TPA along with Dexamethasone and choline magnesium trisalicylate.
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Compositions and Methods Modulating Mg29 for the Treatment of Diabetes

Disclosed herein are compositions and methods for treatment of muscle dysfunction, including diabetes. In addition, the invention relates to therapeutic compositions comprising nucleotides and/or polypeptides of the invention in combination with a pharmaceutically acceptable carrier, wherein the composition facilitates the treatment of skeletal musle disorders. Moreover, the invention relates to the treatment and/or prevention of pathological conditions associated with altered intracellular Ca2+
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Rna Targeting in Alpha-synucleinopathies

Therapies and assays to screen for small molecules that can have therapeutic use in the control of neurodegenerative diseases such as Parkinson's and other alpha-synucleinopathies.
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Inhibition of Quorum Sensing-mediated Processes in Bacteria

Methods are provided for identifying molecules that can be used to positively and negatively manipulate quorum-sensing-mediated communication to control bacterial behavior. Small-molecule antagonists that disrupt quorum-sensing-mediated activities are identified. Methods are provided for disrupting detection of acyl- homoserine lactone autoinducer in Gram-negative bacteria by contacting the bacteria with the antagonists. Methods of inhibiting quorum sensing-mediated activity in Gram-negative bac
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Treatment of Viral Infections By Modulation of Host Cell Metabolic Pathways

Alterations of certain metabolite concentrations and fluxes that occur in response to viral infection are described. Host cell enzymes in the involved metabolic pathways are selected as targets for intervention; i.e., to restore metabolic flux to disadvantage viral replication, or to further derange metabolic flux resulting in "suicide" of viral-infected cells (but not uninfected cells) in order to limit viral propagation. While any of the enzymes in the relevant metabolic pathway can be selecte
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Methods for Predicting Disease Outcome in Patients with Colon Cancer

Closures for containers and methods for using same are provided. In a general embodiment/ the present disclosure provides a closure having a top portion (12), a bottom portion (14) and a side portion (16), an aperture (18) extending though the closure, a projection (20) extending from the closure and at least two rib members (36) on an interior of the projection. The projection may also include a cover (22). In another embodiment, a method for using a closure includes inserting a. spike member i
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Inhibition of Glycerol-3-phosphate Acyltransferase (gpat) and Associated Enzymes for Treatment of Viral Infections

A method of treating or preventing a viral infection in a mammal by administering a compound or pharmaceutically acceptable derivative thereof that inhibits a phosphatidic acid synthesis enzyme
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Angiogenic Resorcinol Derivatives

Novel resorcinol derivatives and methods of preparation and use are presented. These compounds can stimulate angiogenesis as a biological function triggered by the activation of one cannabinoid receptor distinct from CB1 and CB2. Thus, these compounds are specific ligands for one cannabinoid receptor distinct from CB1 and CB2. The invented compounds, when administered in a therapeutically effective amount to an individual or animal, results in a sufficiently high level of that compound in the in
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Antimicrobial Compounds

This disclosure relates to compositions including certain compounds identified by a quantitative, high throughput assay to be effective in inhibiting the ability of a bacterium to kill a host organism, as well as methods for using these compounds for treating bacterial infections.
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Methods of Treating Fungal Infections.

Methods of identifying compounds that potentiate the activity of antifungal agents, potentiators identified by these methods, and methods of using potentiators to treat fungal infections are disclosed.
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Synthetic Triterpenoids and Methods of Use in the Treatment of Disease

The present invention concerns methods for treating and preventing renal/kidney disease, insulin resistance/diabetes, fatty liver disease, and/or endothelial dysfunction/cardiovascular disease using synthetic triterpenoids, optionally in combination with a second treatment or prophylaxis.
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Method for Selectively Inhibiting the Activity of Acat1 in the Treatment of Alzheimer's Disease

The present invention features methods for decreasing the size and density of amyloid plaques, decreasing cognitive decline associated with amyloid pathology, and treating Alzheimer's disease by selectively inhibiting the activity of Acyl -CoA: Cholesterol Acyltransferase 1, but not Acyl -CoA: Cholesterol Acyltransferase 2.
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Female Reproductive Tract and Anal Prophylaxes

The present invention provides methods for boosting mucosal immunity in the female reproductive tract of pre- and post -menopausal women using a TGF-beta inhibitor, a Selective Estrogen Receptor Modulator, and/or a recombinant commensal bacterium that expresses endogenous microbicides into the intestinal tract or reproductive tract of a subject. It also provides methods for boosting innate and adaptive immunity by providing a glucocorticoid. Methods for preventing sexually transmitted infections
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System and Method for Providing Patient Registration Without Fiducials

A method for providing patient registration without fiducials comprises the steps of: spatially placing an ultrasound image of a patient randomly at different starting positions relative to a preoperative image; creating an independent registration corresponding to each different starting positions, by optimizing a spatial transformation between the preoperative image and the ultrasound image to provide a first batch of registrations; executing a second registration to fine-tune the alignment be
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Compositions and Methods for Treating Cancer

The present invention embraces bis-acridine and bis- quinoline intercalators having a bis (4-aminophenyl) ether tether, and diglycolate salts thereof, for selectively decreasing colon cancer and glial cell proliferation and preventing or treating colon cancers and gliomas.
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Compositions and Methods for Treating Pancreatic Cancer

The present invention provides a method of treating pancreatic cancer by inhibiting the activity cyclin D1 activity in tumor cells. The invention is based on the finding that cyclin D1 shRNA molecules are capable of attenuating tumor growth and interfering with tumor angiogenesis.
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Compositions and methods for preventing or treating a viral infection

The present invention is a composition for enhancing the immunogenicity of viral vaccine. The composition encompasses a viral vaccine in combination with at least one toll-like receptor and, in particular embodiments, an anti-CD40 antibody. The compositions of the instant invention find application in the prevention or treatment of a viral infection.
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Monocyclic Cyanoenones and Methods of Use Thereof

The present invention features monocyclic cyanoenone compositions and methods for using the same in the treatment of diseases such as cancer, inflammatory diseases and neurodegenerative diseases.
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Methods for diagnosing and treating neuroendocrine cancer

The present invention relates to a method for diagnosing neuroendocrine cancers via detecting the presence of N-methyl D-asparate-associated (NMDA) glutamate receptors type 1 and/or type 2. Methods for preventing and treating neuroendocrine cancers are also disclosed.
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Animal model and compounds identified via this model for treatment of chronic pain

An animal model for chronic pain, and in particular lower back pain, is provided. Methods of identifying agents and reducing chronic pain with identified agents such as methotrexate are also provided.
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Absorbable Bone Adhesive Applicator

The instant invention provides of a novel method for repairing bone defects using a polymer blend as well as apparatus for performing such a method. The method includes dispensing a polymer blend either directly on the bone tissue as the fixation means; or secondly in combination with a bone plate, the polymer acting as an adhesive
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System and Method for Identification of Fingerprints and Mapping of Blood Vessels in a Finger

In accordance with an embodiment of the invention, there is provided an apparatus for characterizing and identifying a human. The apparatus comprises a light imaging device that images topography of a surface of a portion of human anatomy, and an infrared imaging device that images infrared radiation of the same portion of human anatomy. The light imaging device and the infrared imaging device are rotatable about at least one axis, each of the at least one axis extending through the portion of t
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Polymeric antioxidants

Antioxidant polymers of the present invention comprise repeat units that include one or both of Structural Formulas (I) and (II): wherein: R is -H or a substituted or unsubstituted alkyl, acyl or aryl group; Ring A is substituted with at least one tert-butyl group or substituted or unsubstituted n-alkoxycarbonyl group; Ring B is substituted with at least one -H and at least one tert-butyl group or substituted or unsubstituted n-alkoxycarbonyl group; Rings A and B are each optionally substituted
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Inhibitors of Cyclic Amp Phosphodiesterases

Recombinant fission yeast cells and methods of using them are described, which provide for identification of chemical and biological inhibitors or activators of a target exogenous phosphodiesterase (PDE). The invention provides, in some aspects, compounds that inhibit cAMP PDE activity and compositions that include such compounds. The invention, in part, also includes methods of using cAMP PDE-inhibiting compounds in the treatment of cAMP PDE-associated diseases and/or disorders.
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Sh2 Domain Profiling to Characterize Tyrosine Phosphorylation Signaling in Cancer

A phosphoproteomic method termed SH2 profiling to characterize phosphotyrosine (pTyr) signaling in lung cancer. This method provides quantitative values for the phosphorylated binding sites for Src Homology 2 (SH2) domains, which the cell uses to relay signals from tyrosine kinases. Lung cancer cell lines with known mutational status of the epidermal growth factor receptor (EGFR) and Ras were profiled. Changes in SH2 domain binding were characterized in response to the EGFR inhibitor erlotinib,
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Protein-based Artificial Retinas

Multilayer protein films are provided, which comprise native bacteriorhodopsin and/or specialized bacteriorhodopsin mutants as the photoactive element. Also provided are artificial subretinal and epiretinal implants carrying such bacteriorhodopsin films, as well as methods for making and using the same, for example, to treat retinal diseases and conditions.
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Dialysis Adapter Cell and Method of Use

A dialysis adapter cell includes a housing comprising a plurality of pillars extending between and attached to a top plate and a bottom plate to form a hollow receptacle, wherein the top plate comprises an aperture configured to provide access to the hollow receptacle; a dialysis membrane disposed about the housing and sealingly engaged to the top plate and the bottom plate; and a platform in physical communication with the bottom plate and configured to position the dialysis adapter cell in a s
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Therapeutic Applications of Fatty Acid Amide Hydrolase Inhibitors

Fatty acid amide hydrolase (FAAH) is an enzyme responsible for the degradation of oleamide (an endogenous sleep-inducing lipid) and anandamide (an endogenous ligand for cannabinoid receptors). Disclosed herein are potent inhibitors of FAAH and methods for their use for treating a variety of disorder, including hypertension and cardiac hypertrophy.
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Methods of Identifying Insect-specific Spider Toxin Mimics.

Disclosed herein are methods of identifying a candidate molecule that mimics at least a portion of the three-dimensional structure of a rU-ACTX-Hvla insecticidal toxin, the method comprising providing a molecular model made from the atomic co-ordinates for the rU-ACTX- HvIa insecticidal toxin as disclosed herein, using the molecular model to identify a candidate molecule that mimics the three-dimensional structure of the rU-ACTX-Hvla insecticidal toxin; and providing the candidate molecule that
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Complexes of alpha (2) macroglobulin and antigenic molecules for immunotherapy

The present invention relates to complexes of alpha (2) macroglobulin associated with antigenic molecules for use in immunotherapy. The invention relates to methods for using such compositions in the diagnosis and treatment of immune disorders, proliferative disorders, and infectious diseases.
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Novel Albumin-free Factor Viii Formulation

PROBLEM TO BE SOLVED: To provide a factor VIII formulation for a treatment that is stable in the absence of albumin or other proteins. ; SOLUTION: The factor VIII composition formulated without adding albumin comprises the following excipients in addition to the factor VIII: 4-10% of a filler selected from the group consisting of mannitol, glycine and alanine; 1-4% of a stabilizer selected from the group consisting of sucrose, trehalose, raffinose and alginin; 1-5 mM of a calcium salt; 100-300 m
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Factor Viii Formulations

A Factor VIII (FVIII) composition formulated such that NaCl is not present in the final formulation or is present in trace amounts, which allows for a concomitant reduction in the lyophilization cycle time and increased stability of the lyophilized FVIII.
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Methods and Compositions for Inhibiting Atherosclerosis and Vascular Inflammation

Disclosed herein are compositions and methods for reducing inflammation associated with atherosclerosis and/or vascular inflammatory disease. The methods include administering to a subject in need of treatment for atherosclerosis and/or vascular inflammation a pharmaceutically effective amount of an inhibitor of the receptor activity of the S1P2 receptor. Also included are compositions including an S1P2 receptor antagonist and a pharmaceutically acceptable excipient.
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Compounds for Lysosomal Modulation and Methods of Use

Compounds useful for promoting lysosomal processes and thereby ameliorating the disruption of cellular and functional integrity induced by Ass and other protein and glycoconjugate species are provided. Methods for the treatment of neurodegenerative diseases that involve protein accumulation and aggregation in the brain, such as Alzheimer's, Parkinson's and Huntington's Disease, are also provided.
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Compositions and Methods for the Inhibition of Hepatitis C Viral Replication with Structural Analogs

Compositions and methods for the inhibition of viral replication are provided. In some embodiments, the compositions include a nucleic acid sequence that is identical to a region of the hepatitis C virus (HCV) genome. In other embodiments, the compositions may include a nucleic acid sequence that has at least about 45% to about 95% sequence identity to the native HCV sequence and that has a similar secondary and tertiary structure to the native HCV sequence. In other embodiments, the composition
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Using Heat Shock Proteins to Improve the Therapeutic Benefit of a Non-vaccine Treatment Modality

The present invention relates to methods of improving a treatment outcome comprising administering a heat shock protein (HSP) preparation or an alpha-2-macroglobulin (alpha2M) preparation with a non-vaccine treatment modality. In particular, an HSP preparation or an alpha2M preparation is administered in conjunction with a non-vaccine treatment modality for the treatment of cancer or infectious diseases. In the practice of the invention, a preparation comprising HSPs such as but not limited to,
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Method for Obtaining Human Microglial Precursor Cells from Pluripotent Stem Cells

The present invention relates to a method for obtaining human microglial precursor cells, comprising: (a) providing a ceil population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like
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Sensor Arrangement

The invention relates to a sensor arrangement for detecting electromagnetic radiation whose wavelength in or near the infrared range, with at least one measuring chamber (2), which is filled with the radiation to be measured selectively absorbing fluid, the measuring chamber (2) by a windows (3) sealed entry port for the radiation and part of the Messkammerwandung having a flexible membrane (M) is designed such that, when a volume change in thethe measuring chamber (2) contained fluid is deforme
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Anticancer Agent

The present invention relates to a pharmaceutical composition for the therapeutic and/or prophylactic treatment of cancer and/or metastasis thereof comprising an amino acid sequence related to a Wech protein and/or a nucleic acid sequence of the gene encoding for the Wech protein.
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Apparatus and Method for Detecting Triacetone Triperoxide

The present invention relates to an apparatus and method for fast and secure online detection of triacetone (TATP).
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INHIBITORS OF THE NITRATION OF AMYLOID ss PEPTIDES AND THEIR USES IN THE DIAGNOSIS AND TREATMENT OF ALZHEIMER'S DISEASE

The present invention relates to a method for identifying an inhibitor of the aggregation of amyloid-ss peptide (Ass), comprising the steps of a) contacting at least one Ass-peptide and/or the nitrated forms thereof with at least one candidate inhibitor that potentially specifically binds to a region in said Ass-peptide capable of being nitrated, and b) detecting said inhibitor specifically binding to said region in said Ass-peptide through detecting a lack of or a reduced aggregation of said at
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Cytohesin Inhibitors

The present invention relates to a pharmaceutical preparation comprising as an active ingredient a primary amide according to the general formula (1) and/or a secondary amide according to the general formula (2) and their use as a medicament.
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New nucleic acid molecules and polypeptides involved in lipid metabolism

The present invention relates in a first aspect to polypeptides useful for the prevention or treatment of various diseases. In particular, the present invention relates to a polypeptide containing a homeodomain for the prevention or treatment of lipid-based metabolic diseases, cancer, (neuro)degeneration, autoimmunity, sepsis, cystic fibrosis, diabetes, or for modulating the immune system in an individual or as an anti aging compound. In another aspect, the present invention relates to nucleic a
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PPAR gamma agonists for treating illnesses with pathophysiological participation of TH17 lymphocytes

Use of one or more peroxisome proliferator-activated receptor (PPAR)-gamma agonists for treating a disease in which T helper 17 (TH17) cells have a pathophysiologically relevant function, is claimed. - ACTIVITY : Antiarthritic; Antirheumatic; Antipsoriatic; Neuroprotective; Antiinflammatory; Gastrointestinal-Gen.; Antiulcer; Dermatological; Immunosuppressive; Cerebroprotective; Antiasthmatic; Cardiant; Vasotropic. - MECHANISM OF ACTION : PPAR-gamma agonist; ROR-gamma t factor inhibitor.
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Adenine Receptor Ligands

The invention relates to adenine receptor ligands useful for treating, alleviating and/or preventing diseases and disorders related to adenine receptor function as well as pharmaceutical compositions comprising such compounds and methods for preparing such compounds. The invention is further directed to the use of these compounds, alone or in combination with other therapeutic agents, for the alleviating, preventing and/or treating diseases and disorders, especially the use as antinociceptive or
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Anti-HSV antibody

The invention relates to an anti-HSV antibody as defined in the claims, a pharmaceutical composition comprising of an effective amount of the said antibody, an expression vector comprising of a nucleotide sequence which encodes the said antibody, a host cell comprising of the said nucleotide sequence, a hybridoma cell capable of producing the said antibody and the use of the said antibody as a drug, in particular to use for the manufacture of a drug for the prophylactical or therapeutical treatm
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Pharmaceutical Composition Containing Cannabinoid-receptor Antagonists

The present invention relates to the use of cannabinoid-receptor 2 (CB2R)-antagonists for the prophylaxis and/or treatment of pathogen, in particular, parasitic protozoa, caused or induced cerebral diseases, disorders or conditions including secondary and accompanying diseases, disorders or conditions, like encephalitis. In a preferred embodiment, said CB2R-antagonist is a 3-pyrazole-carboxamide derivative. In a further aspect, the present invention provides pharmaceutical compositions comprisin
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In-vivo Stimulation Measuring Probe

The invention relates to a measuring probe and a system for the targeted optical in-vivo stimulation of nerve cells and for the electrical measurement of nerve cell pulses. The measuring probe comprises at least one measuring needle for automatically controlled, motorized in-vivo insertion into a brain of a vertebrate, there being mounted on the needle at least one electrical measuring pick-up for contacting at least one brain nerve cell so as to measure its activity. Moreover, the measuring pro
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Androgenetic Alopecia

The present invention relates to methods for testing for a predisposition or presence of androgenetic alopecia comprising testing a sample obtained from a prospective patient or from a person suspected of carrying a predisposition for androgenetic alopecia. The invention also relates to a genetic marker for androgenetic alopecia characterised in that the genetic marker is located in a chromosomal region of human chromosome 20p11 and wherein the genetic marker is selected from the group consistin
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POI Prevention

A method is provided, comprising administering at least one guanylhydrazone or salt thereof or a combination thereof to a subject to prevent or ameliorate in said subject at least one of postoperative intestinal inflammation, postoperative ileus, ischemia reperfusion injury, or a combination thereof.
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Methods and Compositions for Promoting Regeneration By Increasing Intracellular Sodium Concentration

The invention provides methods and compositions for increasing the intracellular sodium concentration in a cell.
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Method for Assessing Potential for Tumor Development and Metastasis

The present invention generally provides methods for assessing the potential of tumor formation and/or metastasis using a combination (e.g., a ratio) of the number of circulating tumor cells and the number of circulating cells exhibiting autofluorescence within a selected wavelength region (e.g., red autofluorescence). In one aspect, it is directed to a method for providing likelihood of occurrence of a primary and/or a metastatic cancerous tumor in an animal, which comprises inoculating the ani
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Atoxic Recombinant Holotoxins of Clostridium Difficile As Immunogens

Atoxic Clostridium difficile toxin proteins were expressed in an endotoxin-free Bacillus system top develop a vaccine to reduce incidence and severity of C. difficile infection (CDI). Immunogens evaluated as potential vaccine candidates are mutated toxin A (encoded by TcdA) and toxin B (TcdB), and a rationally designed chimeric protein containing full-length TcdB protein except that the receptor binding domain is replaced with that of TcdA (designated as cTxAB). A small deletion (97 amino acids)
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Inhibitors of Extracellular Hsp90

The present invention describes the inhibitors of extracellular Hsp90. The inhibition of extracellular Hsp90 leads to a reduction of the invasiveness of the tumor cells. Furthermore, the invention relates to the use of molecules inhibiting extracellular Hsp90 function for the manufacture of a medicament for the treatment or prevention of invasion and/or metastatic potential of cancer cells.
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Wound Healing Peptides and Methods of Use Thereof

Methods and compositions for the treatment of wounds in a mammalian subject are provided. Particularly, novel polypeptides and encoding nucleic acids that stimulate keratinocyte and endothelial cell motility and/or proliferation are provided.
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Methods, compositions and kits for vegetative cell-based vaccines and spore-based vaccines

Methods for immunizing a subject to an antigen of an infectious agent, a tumor, or an allergen are provided, using vegetative cytoplasmic expression of the antigen or spore surface display of the antigen, and contacting the subject with a composition including a spore or a vegetative cell or both with or without an adjuvant. Also included are thermally-stable vaccine compositions using the method described above and kits including the compositions.
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Matrix for the production of ligaments, tendons and other tissue

The present invention provides a novel silk-fiber-based matrix having a wire-rope geometry for use in producing a ligament or tendon, particularly an anterior cruciate ligament, ex vivo for implantation into a recipient in need thereof. The invention further provides the novel silk-fiber-based matrix which is seeded with pluripotent cells that proliferate and differentiate on the matrix to form a ligament or tendon ex vivo. Also disclosed is a bioengineered ligament comprising the silk-fiber-bas
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Multivalent Vaccine Vector for the Treatment and Inhibition of Viral Infection

The present invention features compositions and methods for the treatment or inhibition of one or more viruses using a recombinant viral vector that encodes and expresses at least one viral gene from two or more viruses. The viral genes encoded within the recombinant viral vector may be gene sequences from different viruses or may be sequences from different strains of the same virus.
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Compositions and Methods for Treating Ebola Virus Infection

The compositions and methods of the invention described herein provide treatments against Ebola virus infection by expressing gene(s) from the Ivory Coast ebolavirus (ICEBOV) species in a recombinant viral vector.
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Pre-or Post-exposure Treatment for Filovirus Or Arenavirus Infection

The compositions and methods of the invention described herein provide pre- or post-exposure treatments against filovirus or arenavirus infection by expressing one or more genes (e.g., two ore more genes) from filoviruses or arenaviruses in a delivery vehicle (e.g., a recombinant viral vector or a liposome).
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Polymeric Biolubricants for Medical Use

The present invention provides branched polymers which can be used as lubricants or shock absorbers in vivo. For example, the inventive polymers can be used as viscosupplements, viscoelastics, tissue space fillers, and/or anti-adhesive agents. Also provided are pharmaceutical compositions comprising the inventive polymers and methods of using them including, for example, in the treatment of arthritic and sport-injured knee joints; in reconstruction or cosmetic procedures, intervertebral disc rep
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Supramolecular Bioconjugates

The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to a sequence within said plurality of first nucleic acids. To assemble a supramolecular bioconjugate, one or more sets of bioreactive agents are coupled to the plurality of mediators, forming a plurali
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Metabolic and Cardioprotection By the Myokine Follistatin-like 1 Polypeptide

Described herein are methods and compositions related to the discovery that the Follistatin-like 1 protein (Fstl-1) has metabolic and cardioprotective effects in vivo. Fstl-1 and portions and derivatives or variants thereof can be used to treat or prevent metabolic diseases or disorders and to treat or prevent cardiac damage caused by interrupted cardiac muscle blood supply.
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Identification of Novel Pathways for Drug Development for Lung Disease

The invention provides the identification of oncogenic pathways activated in cytologically normal airway cells of individuals having or at risk of having lung disease, as well as specific gene expression patterns (biomarkers) associated with pathway activation. These biomarkers and pathways may provide prognostic and/or diagnostic indicators in lung disease, e.g., lung cancer. Additionally, these pathways and biomarkers may provide therapeutic targets for the treatment of lung disease, as well a
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Knee Brace with Expandable Members and Method of Using the Same

A knee brace (300) has a body (310) supporting first, second, and third expandable members (330). When expanded, the first, second, and third expandable members are positioned on the brace body to provide a counteracting force to the adduction moment of the knee.
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Reca Inhibitors and their Uses As Microbial Inhibitors Or Potentiators of Antibiotic Activity

The present invention provides RecA inhibitors, compositions containing them, systems for identifying or characterizing them, and methods of using them.
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Cell-specifically Effective Molecules on the Basis of Sirna and Application Kits for the Production Thereof and Use Thereof

1. Cell-specifically effective molecules on the basis of siRNA and application kits for the production thereof and use thereof. 2.1. The aim of the invention is to create a link between siRNA and one or more peptides, wherein the siRNA is activated after peptide separation, without the remaining linkers and/or remaining peptide residues significantly impairing the biological efficacy of the siRNA activated in the cell. 2.2. According to the invention, the peptide or peptides (3) are coupled to t
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Method and Device for Rapid Detection of Bacterial Antibiotic Resistance/susceptibility

Described herein is a method and a device for expediting delivery of an agent to a damaged bacterial cell. In one embodiment, the methods and devices are useful for screening candidate antibiotics. In another embodiment, the methods and devices described herein are used to determine susceptibility of bacteria to an antibiotic. The methods also provide a method for determining an appropriate antibiotic to treat an individual having a bacterial infection.
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System for Assessing the Efficacy of Stored Red Blood Cells Using Microvascular Networks

A system for assessing the microvascular fitness of a sample of stored red blood cells. The system has a network device having at least one network unit. The network unit has a single inlet and a single outlet for the sample and a plurality of microchannels. The plurality of microchannels receive the sample from the single inlet and drain the sample into the single outlet. The network unit includes an aspiration pressure means for providing movement of liquid sample through the at least one netw
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Apparatus and Method of Analyzing Arterial Plaque

A method of identifying arterial plaque analyzes arterial plaque using one or more non-invasive tests to determine if the plaque has any of a plurality of hallmarks that are predictive of disruption. The one or more tests do, in fact, test the plaque for the plurality of the hallmarks. The method then formulates a vulnerability quantity as a function of the determined hallmarks. The vulnerability quantity identifies whether the plaque is vulnerable to disruption.
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Pimap39 Modulates Inflammatory Response

The present invention relates to a novel peptide sequence named PIMAP39 (herein referred to as SEQ ID NO.: 1 ) and methods of use of the novel sequence and functional variants thereof. The present invention also relates to methods for reducing and/or modulating inflammatory responses by administration of the peptide of the present invention. Furthermore, the present invention relates to the modulation of the expression of cytokines effected as part of an inflammatory response by administration o
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Tetrahydropyranonaphthyridines Derivatives, Pharmaceutical Compositions and Therapeutic Treatment Thereof

This invention relates to tetrahydropyranonaphthyridines derivatives having formula (III) or IV: and analogues of the tetrahydropyranonaphthyridines derivatives. The invention also relates to pharmaceutical compositions comprising these compounds and methods for treatment of tuberculosis using these compounds.
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TREATMENT OF IgE-MEDIATED DISEASE

The methods and compositions described herein are based, in part, on the discovery of a polypeptide of soluble CD23 (sCD23) that binds and sequesters IgE. Thus, the sCD23 peptides, polypeptides and derivatives described herein are useful for treating conditions or disorders involving increased IgE levels such as e.g., allergy, anaphylaxis, inflammation, lymphoma, and certain cancers.
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Delivery of H2 Antagonists

The present invention provides improved systems and methods for the local delivery of H2 antagonists. The inventive methods include topical administration of an effective amount of a H2 antagonist encapsulated in liposomes. In certain embodiments, the H2 antagonist, for example, Cimetidine, is encapsulated into paucilamellar liposomes, such as NOVASOME TM microvesicles. Also provided are pharmaceutical compositions comprising liposome-encapsulated H2 antagonists. The methods and compositions of
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Thods and Compositions for Preventing Adhesion

This invention provides methods and compositions for preventing post-surgical adhesion formation based on use of an interleukin-16 (IL-16) antagonist, including an IL-16 antagonist peptide and/or an IL-16 antagonist antibody.
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S Isomers of Alpha-methyl Hydrocinnamic Acid for the Treatment of Blood Disorders

The present intervention generally relates to compositions comprising S-isomer of alpha-methyl- hydrocinnamic acid for the treatment and/or prevention of blood disorders and blood deficiencies, as well as methods for treating blood disorders and/or blood deficiency in a subject by administering a composition comprising S-isomer of alpha-methyl-hydrocinnamic acid to the subject to ameliorate at least one symptom associated with the blood disorder. Another aspect relates to administration of an S-
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Small Molecule Inhibitors of Hepatitis C Virus

The invention provides derivatives and analogues of triazatetracyclo[7.7.0.01,13,.02,7]-hexadeca-2,4,6,10,12-pentaenes, 5,7,11-triazatetracyclo[8.7.0.01,6.012,17] -heptadeca- 6,8,12,14,16-pentaenes, pharmaceutical compositions comprising these compounds and methods for treatment of hepatitis C viral infections using these compounds.
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Glutamine Endopeptidases D'espÈce Rothia Et Utilisation De Celles-ci

Disclosed are glutamine endopeptidase enzymes from Rothia sp. bacteria that are naturally associated with the oral cavity, formulations comprising the glutamine endopeptidase enzymes and the use thereof for the treatment, prevention of allergic reaction and diagnosis of gluten allergy related diseases such as Celiac Sprue, gluten allergy and/or dermatitis herpetiformis
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Methods for Treating Viral Disorders

Disclosed are methods of treating viral disorders via the administration of an inducing agent and an anti-viral agent. In one embodiment, the inducing agent and the anti-viral agent are administered for about five days, and the anti-viral agent is subsequently administered without the inducing agent for an additional period of about sixteen days for a total cycle of about 21 days.
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Compositions for the treatment of blood disorders

The invention relates to compositions containing chemical compounds and compositions containing steel factor which stimulate the expression of hemoglobin or globin protein such as embryonic or fetal globin, or the proliferation of hemoglobin expressing and other cells. These compositions can be used to treat or prevent the symptoms associated with anemia, sickle cell diseases, thalassemia and other blood disorders. The invention also relates to methods for administering these compositions to pat
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Lipoxin Analogs and Methods for the Treatment of Periodontal Disease

This invention provides new lipoxin analogs, compositions containing analogs, and methods of using these compounds and compositions for treating and preventing oral inflammation, including gingivitis, periodontitis, and other forms of periodontal disease. The invention also provides for methods of treating and preventing oral inflammation, including gingivitis, periodontitis, and other forms of periodontal disease with compositions containing COX-2 inhibitors. Further, the invention provides met
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Circulating Mirnas As Non-invasive Markers for Diagnosis and Staging in Prostate Cancer

The present invention relates to a method for staging prostate cancer in a subject suffering from prostate cancer, said method comprises (a) determining the amount of at least one miRNA selected from the group consisting of miRNA-375, miRNA-141, miRNA-200b, miRNA-516a-3p and miRNA9*, or the amount of a precursor molecule in a sample from said patient and (b) comparing the, thus, determined amount with a reference amount. Moreover, the present invention is concerned with a method for identifying
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Human Antibodies Against Hepatitis C Virus (hcv) Uses Thereof.

Isolated human monoclonal antibodies which bind to hepatitis C virus (HCV), and related antibody-based compositions and molecules, are disclosed. The human antibodies can be produced in a transfectoma or in a non-human transgenic animal, e.g., a transgenic mouse, capable of producing multiple isotypes of human monoclonal antibodies by undergoing V-D-J recombination and isotype switching. Also disclosed are pharmaceutical compositions comprising the human antibodies, non-human transgenic animals,
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Spinal Muscular Atrophy (sma) Treatment Via Targeting of Smn2 Splice Site Inhibitory Sequences

The present invention is directed to methods and compositions capable of blocking the inhibitory effect of a newly-identified intronic inhibitory sequence element, named ISS-N1 (for "intronic splicing silencer"), located in the SMN2 gene. The compositions and methods of the instant invention include oligonucleotide reagents (e.g., oligoribonucleotides) that effectively target the SMN2 ISS-N1 site in the SMN2 pre-mRNA, thereby modulating the splicing of SMN2 pre-mRNA to include exon 7 in the proc
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Novel Metastasis Suppresor Genes and Uses Thereof

The invention relates to methods for detecting a metastatic cancer, methods for inhibiting tumor metastatic properties, methods for treating tumor metastasis, methods for identifying agents for the treatment of tumor metastasis, and methods for screening for modulators of tumor metastatic properties.
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Lipid-amino Acid Conjugates and Methods of Use

N-fatty acid-amino acid conjugates and J2 prostanoid-amino acid conjugates are disclosed along with methods for making such conjugates and methods of using these conjugates in the treatment of conditions that involve dysfunctional lipid metabolism, insulin sensitivity, glucose homeostasis, and/or inflammation.
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Methods for Treating Endoplasmic Reticulum (er) Stress Disorders

The present invention provides methods for treating ER stress disorders and for identifying compounds for treating ER stress disorders.
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Type Iii Secretion Inhibitors, Analogs and Uses Thereof

The invention, in some aspects, relates to compounds and compositions useful for inhibiting Type III secretion systems in pathogenic bacteria, such as Yersinia Pestis. In some aspects, the invention relates to methods for discovering inhibitors of the Type III secretion system and uses of such inhibitors in the treatment and prevention of disease.
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Human Antibodies Against Rabies and Uses Thereof

Human monoclonal antibodies that specifically bind to rabies virus, antigen binding portions thereof, and methods of making and using such antibodies and antigen binding portions thereof for treating rabies virus in a subject, are provided herein.
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Methods and Compositions for the Treatment of Sterile Inflammation

Described are methods and compositions that inhibit IL-1 signalling for the treatment of acute inflammatory response to cell necrosis, and the attendant collateral tissue damage.
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Methods of Monitoring Treatment of Aviremic Hiv-infected Patients

Methods of monitoring the efficacy of intensified highly active anti-retroviral therapy (HAART) treatment in aviremic Human Immunodeficiency Virus (HIV)-infected patients.
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Methods for Identifying Modulators of the Pp2a B56 Beta Regulatory Subunit

The present invention is based at least in part on the discovery that the PP2A B56 beta regulatory subunit plays a role in regulating insulin signaling by directly regulating phosphorylation of AKT-I. Accordingly, the present invention features methods of identifying modulators of the PP2A B56 beta regulatory subunit in assays featuring organisms and/or cells. Further featured are therapeutic methods for the use of PP2A B56 beta regulatory subunit modulators to enhance longevity, to prevent or t
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Methods and Compositions for Reducing Inflammation and Treating Inflammatory Disorders

Methods of treating an inflammatory disorder and inhibiting inflammation by administering an inhibitor of a pH-activated protease are provided.
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Compounds for Modulating Rna Binding Proteins and Uses Therefor

The invention relates to compositions and methods for inhibiting RNA binding proteins (e.g., MEX-3, MEX-5 and POS-1), as well as methods for treating and preventing disorders associated with parasitic infections and inflammatory disorders.
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Methods and Compositions for Treating Neurological Disease

This invention relates to methods and compositions for treating neurological disease, and more particularly to methods of delivering iRNA agents to neural cells for the treatment of neurological diseases.
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Compounds for Modulating Tlr2

The present invention is directed to methods, kits, and uses of inhibitors of LCMV mediated NF-?B activation to treat viral infections and inflammatory conditions.
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Methods for Treating Glioblastoma

Described are methods for treating cancers, e.g., glioblastoma, including administering an inhibitor of Notch signalling, e.g., a gamma secretase inhibitor, in combination with a chemotherapeutic agent.
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Tumour-specific P450 Protein

The discovery that CYP1B1 protein is detectable in a wide range of human cancers of different histogenetic types, but is not detectable in non-cancerous tissues, gives rise to diagnostic methods for detecting tumours based on this protein as a marker, and to the possibility of tumour therapies involving the protein. A diagnostic method may include the steps of: (a) obtaining from a patient a tissue sample to be tested for the presence of cancer cells; (b) producing a prepared sample in a sample
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Detection of Human Immunodeficiency Virus Co-receptor Tropism in Aviremic Subjects

Methods for detecting human immunodeficiency virus (HIV) co-receptor tropism or replication-competent virus in aviremic subjects, and methods of selecting optimal therapies for aviremic subjects.
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Methods for the Treatment Or Prevention of Diabetes Mellitus and Other Metabolic Imbalances

The invention relates to methods for treatment and prevention of a metabolic imbalance, including diabetes mellitus and other related diseases or disorders, using an extract from a fruit of genus Elaeis.
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Irf As a Tumor Suppressor and Uses Thereof

The invention relates to methods for treating BCR/ ABL mediated disorders. The methods of the invention also include monitoring progression of or sensitivity to treatment of BCR/ ABL mediated disorders as well as identifying subjects for the treatment methods of the invention. Screening assays and related products and kits are also encompassed within the invention.
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Microna Compositions in the Treatment of Vegf-mediated Disorders

The invention provides methods of treating diseases caused by the over-production of a VEGF polypeptide by administering miRNA or miRNA inhibitor compositions to decrease at least one activity of a VEGF polypeptide.
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A Method for Preventing Or Alleviating the Noxious Effects Resulting from Toxicant Exposure

The present invention provides a method of using agents which can modulate TRPAl function as counteragents to inhibit the physical effects of chemical irritants/toxicants when given prior to exposure or to lessen the physical effects when administered post exposure, and more specifically, to a method for counteracting the acute physical noxious effects of toxicants, including but not limited to, tear gases, chlorine, hydrogen peroxide, ammonia, phosgene, chloropicrin, isocyanates and mustard gas
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Chimeric Small Molecules for the Recruitment of Antibodies to Cancer Cells

The present invention relates to chimeric chemical compounds which are used to recruit antibodies to cancer cells, in particular, prostate cancer cells or metastasized prostate cancer cells. The compounds according to the present invention comprise an antibody binding terminus (ABT) moiety covalently bonded to a cell binding terminus(CBT) through a linker and optionally, a connector molecule.
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Treatment and Prevention of White Matter Injury with Katp Channel Activators

The present invention includes a method of treating or preventing a CNS white matter injury in a patient in need thereof. The invention also includes a method of stimulating proliferation of a CNS cell in a patient in need thereof.
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Compounds and Methods for Treating Viral Encephalitis

The invention includes compositions, methods, and techniques for influencing or otherwise altering or regulating signaling that emanates from ligands, receptors, or pathways of TLR7, and/or IL- 12, and/or IL-23, and/or IL- 17, singly or in any combination. The invention includes inhibiting ligands, receptors, or pathways of TLR7, and/or IL- 12, and/or IL-23, and/or IL- 17 singly or in any combination using pharmacologic means, which includes agonist binding, antagonist binding and subsequent eff
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Methods and Compositions for the Detection and Treatment of Preeclampsia

Methods, kits and compounds are provided that relate to the diagnosis, treatment, and/or prevention of preeclampsia.
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Activated Wnt-beta-catenin Signaling in Melanoma

The present invention is directed to a method of determining the prospects for survival of a melanoma patient. This method involves providing a biological sample from a patient diagnosed with melanoma, determining the level of an indicator of Wnt/ss-catenin activation in the sample, comparing the level of the indicator of Wnt/ss-catenin activation in the sample against a standard level of the indicator of Wnt/ss-catenin activation correlated to survival of melanoma, and determining a patient's p
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Modulation of Sirt1 Expression

Methods are provided for reducing SIRT1 expression by administering a SIRT1 inhibitor. Also provided are methods for treating cardiovascular and metabolic disorders in a subject or delaying or preventing risk factors thereof through the reduction of SIRT1 expression. The present invention is also directed to methods of decreasing lipid levels in a subject or for preventing or delaying the onset of a rise in lipid levels in a subject, comprising administering to said subject a SIRT1 inhibitor.
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Apoptosis-Based Evaluation of Chemosensitivity in Cancer Patients

Induction of apoptosis in target cells is a key mechanism by which chemotherapy induces cell killing. An in vitro system has been established for determining carboplatin and paclitaxel (Taxol) chemosensitivity of epithelial ovarian cancer cells, where measurements of caspase-3 activation are surrogate markers for activation of chemotherapy-induced programmed cell death. To validate the assay as a predictor of clinical chemotherapy-induced programmed cell death. To validate the assay as a predict
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Method and composition for treating immune complex associated disorders

The present invention provides methods and compositions for treating immune complex associated diseases (ICAD), such as SLE, rheumatoid arthritis, and hepatitis-C related immune complex disease (e.g., cryoglobulinemia) in a subject having an ICAD or at risk for developing ICAD. The invention is based upon the surprising finding that chromatin-containing immune complexes activate autoreactive B cells and dendritic cells by a dual receptor engagement process which, in both cell types, involves a T
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C/CLP Antagonists and Methods of Use Thereof

The invention relates to the novel discovery that antagonizing a C/CLP can be useful for the treatment of diseases associated with the upregulation of one or more C/CLP such as Th2-driven and/or IL-13 mediated inflammatory diseases. Accordingly the present invention provides C/CLP antagonists and also provides compositions and methods for the prevention, management, treatment or amelioration of an inflammatory condition associated with the upregulation of a C/CLP or one or more symptoms thereof
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Methods of Treating Hepatitis

The present invention relates to a method of treating hepatitis in a patient, which includes administering a pharmaceutical composition that includes carbon monoxide to the patient.
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Diagnosis and Treatment of Age Related Macular Degeneration

Methods, compositions and kits for diagnosis and treatment of age related macular degeneration.
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Method for suppressing immune system response to transplanted tissue or cells

Methods are provided for suppressing the immune system response in recipients of transplanted organs, tissues or cells. An extracorporeal quantity of blood from the intended transplant recipient is treated to induce monocytes contained in the blood to differentiate and form dendritic cells. The maturation of the dendritic cells is truncated at a stage where the dendritic cells can inactivate T cell clones which would otherwise generate an undesired immune system response. The immature dendritic
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Compositions and Methods for Treating Or Inhibiting Liver Injury

The present invention relates to the discovery that acetylsalicylic acid (ASA or aspirin), salicylic acid (SA) and related salicylate esters and their pharmaceutically acceptable salts, when coadministered in effective amounts with a drug or other bioactive agent which typically (in the absence of the salicylate compound) produces significant hepatotoxicity as a secondary indication, will substantially reduce or even eliminate such hepatotoxicity. Favorable therapeutic intervention results from
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Herbal Composition Phy906 and Its Use in Chemotheraphy

This invention provides herbal compositions useful for increasing the therapeutic index of drugs, including those used in the treatment of disease, especially viral infections and neoplasms of cancer. This invention provides methods useful for improving the quality of life of an individual undergoing chemotherapy. Furthermore, this invention improves the treatment of disease by increasing the therapeutic index of chemotherapy drugs by administering the herbal composition PHY906 to a person under
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An Objective, Quantitative Method to Predict Histological Subtype in Non-small Cell Lung Cancer

The invention provides a method for determining the histotype of a non-small cell lung cancer tumor which comprises (a) determining the levels of expression of each of thyroid transcription factor-1 cytokeratm 5 cytokeratm-13 and epidermal growth factor receptor in a sample from the non-small cell lung cancer tumor (b) calculating a score based on the levels of expression determined m step (a) and (c) comparing the score obtained m step (b) with a predetermined reference score associated with hi
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Anti-viral nucleoside analogs and methods for treating viral infections, especially hiv infections

The present invention relates to novel compounds according to the general Formulae (I, II, III, IV or V); wherein B is nucleoside base according to the structure Formula (VI); R is H, F, Cl, Br, I, C 1 -C 4 alkyl (preferably CH 3 ), -C N, -C C-R a , Formula (VII); X is H, C 1 -C 4 alkyl (preferably CH 3 ), F,Cl,Br or 1; Z is 0 or CH 2 with the proviso that Z is CH 2 and not O when the compound is according to general formula II, R 3 is -C C-H and R 2 is H or a phosphate, diphosphate, triphosphat
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Nogo Receptor Binding Small Molecules to Promote Axonal Growth

The present invention provides a method for identifying compounds which modulate the interaction of Nogo and Nogo receptor (NgR). The present invention also provides compounds that modulate the interaction of Nogo and Nogo receptor (NgR), the use of such compounds and compositions in the treatment or amelioration of conditions diseases or disorders, such as spinal cord injury, traumatic brain injury, stroke, multiple sclerosis, ALS, Huntington's disease, Alzheimer's disease, Parkinson's disease,
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Methods for Modulating Expression of Creb

Methods are provided for modulating CREB by administering a CREB-specific modulator. Also provided are methods for treating cardiovascular and metabolic disorders in a subject or delaying or preventing risk factors thereof through the modulation of CREB. The present invention is also directed to methods of decreasing lipid levels in a subject or for preventing or delaying the onset of a rise in lipid levels in a subject, comprising administering to said subject a CREB-specific inhibitor.
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Survivin, a protein that inhibits cellular apoptosis, and its modulation

The present invention provides the amino acid of a protein that inhibits cellular apoptosis, herein termed the Survivin protein and nucleic acid molecules that encode Survivin. Based on this disclosure, the present invention provides isolated Survivin protein, isolated Survivin encoding nucleic acid molecules, methods of isolating other members of the Survivin family of proteins, methods for identifying agents that block Survivin mediated inhibition of cellular apoptosis, methods of using agents
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Neovascular-Targeted Immunoconjugates

Immunoconjugates for treating diseases associated with neovascularization such as cancer, rheumatoid arthritis, the exudative form of macular degeneration, and atherosclerosis are described. The immunoconjugates typically consist of the Fc region of a human IgG1 immunoglobulin including the hinge, or other effector domain or domains that can elicit, when administered to a patient, a cytolytic immune response or cytotoxic effect against a targeted cell. The effector domain is conjugated to a targ
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Reactivation of Axon Growth and Recovery in Chronic Spinal Cord Injury

Disclosed are methods of treating chronic nervous system diseases or injuries, e.g., chronic spinal cord injury, using Nogo receptor antagonists, including Nogo receptor-1 (NgR1) polypeptides, Nogo receptor-1 antibodies and antigen-binding fragments thereof, soluble Nogo receptors and fusion proteins thereof, and polynucleotides. Also disclosed are methods of noninvasively monitoring axonal growth during and after treatment with an axonal growth promoting agent.
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Compositions and Methods for Treating Cancer with Attenuated Oncolytic Viruses

Compositions including attenuated oncolytic viruses and methods of their use for the treatment of cancer are disclosed. Some attenuated virus exhibit potential as tumor therapies by exhibiting characteristics including, but not limited to, high selectivity, infectivity, cytotoxicity, or replication index for tumor cells, and/or low infectivity, cytotoxicity, or replication index for normal cells. In preferred embodiments, the ratio of replication of virus in normal cells-tumor cells is about 1:1
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Aqueous Extract of Pieris Brassicae Larvae and Its Use As Antioxidant

The present research respects to the aqueous extract of Pieris brassicae larvae fed with tronchuda cabbage, which possesses antioxidant activity against radical species prejudicial to health and has capacity to inhibit xanthine oxidase, an enzyme involved in gout development. The compounds present in this extract are rare in nature and hard to be synthesized in the laboratory, due to their complex chemical structures. Thus, the present invention proportionate the obtainment of standardized aqueo
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Hydroxyapatite and Bioglass-based Pellets, Production Process and Applications of Thereof

The present invention refers to hydroxyapatite and bioglass-based pellets of homogeneous size and spherical shape, whose interconnective porous structure, in the micrometer range, allows for an enhanced osteoconductivity and osteointegratioh, with specific application as a synthetic bone graft and to the respective production process. The production process is based on the pharmaceutical technology of extrusion and spheronization employing a porogenic agent and applying a sinterization stage. in
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Urinary Gm2 Activator Protein As a Marker of Acute Renal Failure Or the Risk of Developing Acute Renal Failure

The invention relates to a method of determining the risk of developing acute renal failure (ARF) in an individual, or of determining an ARF, and a method of predicting the progression of an ARF, by detecting and/or quantifying the protein Ganglioside GM2 Activator Protein (GM2AP). The failure can be due to the administration of at least one nephrotoxic agent, wherein the nephrotoxic agent can be an aminoglycoside antibiotic as for example gentamicin.
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Glycosylated Indolecarbazoles, Method for Obtaining Same and Uses Thereof

Glycosylated indolecarbazoles, method for obtaining same and uses thereof. This invention relates to rebeccamycin and staurosporine derivatives obtained by means of fermentation of recombinant bacterial strains. The invention also relates to the methods used to obtain the recombinant strains and to produce rebeccamycin and staurosporine derivatives. The invention also relates to bacterial strains that are of use for the production of rebeccamycin and staurosporine derivatives. Lastly, the rebecc
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Oviedomycin Derivatives, Method for Obtaining Same and Use Thereof

The invention relates to oviedomycin derivatives, the method for obtaining same and the use thereof. The invention relates to oviedomycin derivatives obtained by fermentation of recombinant bacterial strains. The invention also relates to the methods used to obtain the recombinant strains and to produce the oviedomycin derivatives. The invention further relates to bacterial strains that can be used to produce oviedomycin derivatives. Said oviedomycin derivatives can be used in the field of human
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Therapeutic Use of the Encoding Sequence of the Carboxy-Terminal Domain of the Heavy Chain of the Tetanus Toxin

The present invention relates to the therapeutic use of the encoding sequence of the carboxy-terminal domain of the heavy chain of the tetanus toxin and of the polypeptide encoded by said sequence, preferably for the treatment of amyotrophic lateral sclerosis (ALS).
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Use of Catecholamine for Differentiation of Stem Cells Into Cardiomyocytes

The present invention relates to the use of catecholamine for differentiation of stem cells into cardiomyocytes and their maturation and a method for obtainment and maturation of these cardiac cells. In addition catecholamine may be used for the preparation of a medicament destined for treatment of cardiac damage.
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USE OF eNOS INHIBITORS IN NERVOUS REGENERATION

The invention relates to the use of inhibitors of the activity and/or expression of the endothelial isoform of the NO synthase enzyme (eNOS) in the preparation of a medicament for increasing the regeneration speed of damaged peripheral nerves in a patient requiring such treatment. The invention also relates to the use of vectors guiding the expression of the eNOS inhibitors in the production of said medicaments, enabling the acceleration of the recovery of neuromuscular communication following a
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Use of Maslinic Acid for Treating Nociceptive, Inflammatory and Neurogenic Pain

Use of maslinic acid for treating nociceptive, inflammatory and neurogenic pain. The present invention relates to the use of maslinic acid and any of the derivatives thereof for treating painful pathological processes of a (1) nociceptive, (2) inflammatory or (3) neurogenic nature by any galenically acceptable means and very especially topically, including compositions that contain maslinic acid, any of the derivates thereof, or natural, synthetic or semi-synthetic mixtures rich in maslinic acid
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Method for Generating a Virtual Simulated Patient

The invention relates to a virtual simulated patient (talk bot) simulating the behaviour of a patient during a medical consultation such that the responses are as realistic as possible, corresponding to the symptoms of at least one real illness, based on diffuse logic and Markov chains.
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Use of Maslinic Acid for the Treatment of Diseases and the Symptoms Thereof By Means of Cox-2 Inhibition

The invention relates to the use of maslinic acid or natural, synthetic or semi-synthetic maslinic-rich mixtures or of a composition containing said acid for the treatment of pathological processes associated with COX-2 activation, intended, inter alia, for the symptomatic and/or regenerative treatment of arthrosis, rheumatoid arthritis, fibromyalgia, sciatica and other articular disorders that are difficult to diagnose. The invention also relates to the topical administration thereof.
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Use of Virgin Olive Oil Polyphenolic Compounds in Cancer Treatment

The invention relates to the use of polyphenol compounds present in extra virgin olive oil for the treatment of cancer, as well as to pharmaceutical compositions containing same and to the use thereof in the production of drugs.
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Composition for Preventing Or Treating Medical Disorders Associated with Angiogenesis and Cell Proliferation

The invention relates to a composition which includes a statin and an agent activating the vascular endothelium. Said composition can be used for producing drugs or pharmaceutical compositions, preferably for preventing or treating medical disorders associated with abnormal cell proliferation and with angiogenesis due to a disease or unwanted angiogenesis such as cancer and arteriosclerosis.
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Use of the Masp-52 Protein for the Diagnosis, Treatment and Prevention of Chagas Disease

The invention relates to the use of the MASP-52 protein, from the mucin-associated surface protein (MASP) family, for the diagnosis, treatment and prevention of Chagas disease, including a method for detecting the presence of the T. cruzi parasite in individuals, a method for obtaining data that can be used for diagnosing Chagas disease and a diagnostic kit containing the elements necessary for analysing the quantity of MASP-52 protein in a biological sample.
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Synthetic Analogues of Merosesquiterpenes and Related Compounds Having Antitumoural Activity

The invention relates to antitumoural properties in respect of solid tumours, such as breast, neuroblastomas, lung, colorectal, hepatic and pancreatic cancers both in humans and in animals, of synthetic compounds having a merosesquiterpenic structure related to marine metabolites of puupehenone type.
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Novel (rs)-7- Or 9-(1,2,3,5-tetrahydro-4,1-benzoxazepin-3-il)-7h Or 9h-purines Having Antitumour Activity

The invention relates to compounds intended for use in the treatment of human and animal tumours. Experimental results obtained on breast tumour and non-tumour cells lines suggest that the selected compounds have a specific selective antitumour activity, rendering same excellent candidates for future use in the treatment of different types of cancers.
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Aqueous Solution for the Preservation of Tissues and Organs

It is provided an improved aqueous solution for the preservation of tissues and organs comprising carvedilol, tacrolimus, and trimetazidine. A synergistic effect is observed for this preservation solution which is particularly effective in marginal organs, such as steatotic livers.
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Method and Kit for Predicting the Chances of Survival of Patents with Mantle Cell Lymphoma

Mantle cell lymphoma (MCL) is a distinct subtype of B-cell non-Hodgkin's lymphoma (NHL-B). The invention provides a method and a kit for predicting the chance of survival of a patient suffering from MCL, based on measuring the level of expression of at least four genes selected from the group comprising: MYC, RAN, POLE2, SLC29A2 and TNFRSF10B in a sample taken from the patient. The method and kit are simple as they involve a minimum number of genes, are quantitative, can be used in routine diagn
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Protein Product for Treatment of Infectious Diseases and Related Inflammatory Processes

The inventors have found that CD6, a member of the Scavenger Receptor Cysteine-Rich (SRSR) superfamily expressed on human lymphocytes binds to Gram-positive and Gram-negative bacteria, as well as to other microbial structures. Thus, a CD6 product is useful for the manufacture of a medicament for therapeutic and/or preventive treatment of an infectious disease or of an inflammatory condition related to an infectious disease or to the presence of a product derived from an infectious agent in a mam
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Method for the Prediction of the Severity of Nuclear Envelope-related Diseases.

Nuclear envelopathies are diseases caused by a malfunction of nuclear envelope associated proteins or lamins. Nuclear envelopathies are heterogeneous group of diseases, ranging from muscle and lipid dystrophies to progeria. Genetic screening does not warrant a proper assessment of the impact of the mutation(s) due to a highly variable penetrance between affected individuals. Here we present a novel method to assess and predict clinical outcome by screening the loss of nucleo-cytoplasmic compartm
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Method for Screening Compounds for the Treatment Or Prevention of Colon Cancer

The invention is in the field of molecular medicine, more in particular in the field of identifying new compounds for the treatment or prevention of colorectal cancer. The invention provides a method for the screening and/or identification of compounds that are capable of preventing or ameliorating the adverse effects of reactive oxygen species on eukaryotic cells. More in particular, the invention provides a method for the identification of an active therapeutic compound for preventing or ameli
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Method for the Treatment of Ischemia Reperfusion Injury

The invention is in the field of thrombosis, haemostasis, cardiology, medicinal chemistry and/or pharmacology. More particularly, it relates to the use of a mutant activated protein C (APC) for the treatment of ischemia reperfusion injury. A method is provided for treating or preventing reperfusion injury following an ischemic event that comprises administering to a subject a pharmaceutical preparation comprising a mutant APC or a precursor thereof. Compositions for use in the present invention
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Diagnostic and Therapeutic Tools for Diseases Altering Vascular Function

The invention relates to diagnostic and therapeutic tools and applications, particularly useful in diseases that affect vascular health and in inflammatory diseases. In particular, said diagnostic and therapeutic tools employ suitable detection or modulation of endothelial glycocalyx.
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Means and Methods for Diagnosing And/or Treating a Subject At Risk of Developing Heart Failure

The present invention relates to a method for identifying a subject at risk of developing heart failure, comprising: (a) determining the level of one or more biological markers in a biological sample of said subject; (b) comparing the level of said biological marker to a standard level of the same biological marker; and (C) determining whether the level of the marker is indicative of a risk for developing heart failure, wherein the biological marker is Krüppel Like Factor 15 (KLF-15) and/or lys
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Two Component Bone Cement Composition for Vertebroplasty

The invention is directed to an intrinsically radio-opaque two component bone cement, comprising a first component which contains at least one acrylate monomer and a second component which contains at least one initiator for the polymerisation of said acrylate monomer, wherein the at least one iodine containing radio-opacity providing polymer is present in at least one of the two components and wherein the cement, when mixed into a slurry has a viscosity, which is sufficiently low to allow injec
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Treatments Involving Glutaredoxins and Similar Agents

The present invention generally relates to treatments involving glutaredoxins. In one aspect, systems and methods of the invention can be used to treat a subject having an oxidative stress condition, for example, airway inflammation or asthma. In some embodiments, a glutaredoxin may be used to treat a subject. Also provided in certain aspects of the present invention are kits for therapies involving glutaredoxins, methods for promoting such therapies, and the like.
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Disease susceptibility

The invention provides a method of assessing the susceptibility of a subject to an anxiety disorder or depression, the method comprising determining whether the subject has a haplotype comprising rs3216799, rs6814934, rs765e048, rs2070950 and rs2070951 with respective alleles '+CT', 'C', 'T', 'C' and 'C'. The invention provides a kit of parts or solid substrate for use in assessing the susceptibility of a subject to an anxiety disorder or depression, the kit comprising or the solid substrate hav
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Organ Protection

The present invention provides compositions, medicaments and methods for use in preventing and/or alleviating the damaging effects of hypoxia and/or reperfusion injury.
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Sphingosine- 1- Phosphate (s1p) Receptor Compounds

The invention relates to compounds, in particular 2-amino- 3, 4, 5, -trisubstituted thiophenes, pharmaceutical compositions containing them and the uses of said compounds and compositions for diseases related to sphingosine-1-phosphate (S1P) receptors, predominantly SlP3 receptors. The diseases include cardiovascular diseases, atherosclerosis, cancer, pulmonary oedema, autoimmune disorders and Adult Respiratory Distress Syndrome.
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Use Larch Wood Material for Treating Inflammation

The application of wood material from a tree of the genus Larix for treatment and prophylaxis of inflammation in humans or animals is described. The present invention is based on the use of raw larch wood material as a medicament and an anti-inflammatory food/ feed supplement for animals and humans, wherein preferably larch sawdust, a by-product of wood industry, is used.
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Method for Detecting a Wound Infection

he present invention relates to a method for detecting a wound infection comprising the steps of : - contacting a sample obtained from a wound with at least two substrates for at least two enzymes selected from the group consisting of lysozyme, elastase, cathepsin G and myeloperoxidase, and - detecting a wound infection when a conversion of the at least two substrates with said at least two enzymes are determined.
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Targets for Retrovirus Associated Diseases

The invention generally relates to retrovirus associated diseases, including diseases caused by human immunodeficiency virus (HIV) or human T-cell leukaemia virus (HTLV). In particular, the invention discloses host proteins targeted by viral proteins in retrovirus associated diseases, and teaches related products and methods useful for the study, assessment and treatment of said diseases.
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Surgical procedure for the treatment of female urinary incontinence: tension-free inside-out transobturator urethral suspension

A new, quick, simple, efficient, safe, and reproducible surgical technique for the treatment of female urinary incontinence, in which the posterior urethra is suspended using a tape passed through the obturator orifices from inside (underneath the urethra) to outside (thigh folds). A variety of specifically designed surgical instruments are utilized to perform this operative procedure.
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Novel isoform of versican and use in diagnosis and therapy

An isolated and purified nucleic acid molecule having a nucleic acid sequence encoding the V4 isoform of versican, or a fragment thereof of at least 30 nucleotides comprising the exon boundary between exons 8a and 9 and a method and a kit for determining the presence and/or status of a tumor in a tissue of a patient by determining the presence and/or level of gene transcription of the V4 isoform of versican in such tissue. The V4 isoform of versican or a fragment thereof can also be used as a me
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Water Soluble Curcumin Compositions for Use in Anti-cancer and Anti-inflammatory Therapy

The present invention relates to the medical field. In a first aspect the present invention relates to novel water soluble cyclodextrin-curcumin complexes having a pharmacological activity, in particular an anti-tumour and/or anti-inflammatory activity, and improved physico-chemical properties. In a second aspect, the present invention relates to a method for preparation of said water soluble curcumin derivatives. The invention further relates in a third aspect to a pharmaceutical composition co
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Combined method for the sequential measurement of (1) the enzymatically active fraction and (2) the total amount of an enzyme

The present invention is related to a combined method and kit (or device) for the sequential measurement of the enzymatically active fraction and the total amount of an enzyme [(such as myeloperoxidase (MPO)] in a sample, and that find improved applications in veterinary and human health fields.
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Method for Generating Islet Beta Cells from Dedifferentiated Exocrine Pancreatic Cells

The present invention relates to an in vitro method for generating insulin-producing beta cells from a population of mammalian cells comprising dedifferentiated exocrine pancreatic cells. The method comprises the step of culturing said dedifferentiated exocrine pancreatic cells in a culture medium in the presence of at least one agent that is able to inhibit the Notch 1 signaling pathway in said dedifferentiated exocrine pancreatic cells, and at least one ligand of the gp130 receptor and/or at l
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Generation of pancreatic progenitor cells

The invention relates to in vitro methods for producing pancreatic progenitor cells and cell populations from cells having definitive endoderm germ layer phenotype. The invention further encompasses so-obtained pancreatic progenitor cells and cell populations, compositions comprising the same, and further uses of said pancreatic progenitor cells and cell populations, such as inter alia for producing functional, insulin-producing beta cells. The invention is of particular relevance for medical ap
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Carboxyethylated Cyclodextrin Polysulfates Useful As Medicaments

This invention relates to novel ss-cyclodextrin polysulfate compounds comprising from one to three 2-carboxyethyl substituents, and at least two sulfates groups per glucopyranose unit, which are useful as active ingredients for the treatment and/or prophylaxis of degenerative joint diseases, osteoarthritis, articular rheumatism, arthrosis or degenerative arthritis, or for the treatment of heparin-induced thrombocytopenia, or for cartilage repair or connective tissue repair.
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The Use of Topoisomerase Type I Inhibitors to Treat Viral Infections

The present invention relates to the use of compounds, inducing isgylation to treat viral infections. More specifically, the invention relates to topoisomerase type I inhibitors, such as camptothecin and camptothecin derivatives to treat viral infections, preferably infections by RNA viruses. The invention relates further to the measurement of the level of ISG15 and or the level of isgylation during or after treatment as a method to measure the efficacy of the antiviral treatment.
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Mucosal Membrane Receptor and Uses Thereof

The invention is based on the identification of aminopeptidase N (APN) as the receptor for F4 fimbriae of enterotoxigenic E. coli (ETEC). Based on the observation that oral administration of F4 fimbriae induces a protective intestinal mucosal immune response against a subsequent challenge with F4 ETEC, and the observation that the internalization of said F4 fimbriae is clathrin-mediated, the present invention provides the characterization of APN as a target useful in: in an in vitro assay to scr
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Synephrine Derivatives for the Treatment of Cancer

This invention relates to a group of synephrine derivatives represented by the structural formula (I), a stereoisomer thereof, a solvate thereof, or a salt thereof, for use in the treatment of cancer, possessing unexpected desirable pharmaceutical properties, in particular for the use in cancer therapy or tumor treatment, especially for glucocorticoid-resistant treatment.
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Compounds for Use in Therapy

A compound of formula (I) for use in the treatment of a disease of the immune system; wherein R is an optionally-substituted alkyl, alkenyl, alkynyl, aryl, arylalkyl or alkylaryl moiety having from 1 to 50 carbon atoms; R1 is an optionally-substituted alkyl, alkenyl, alkynyl, aryl, arylalkyl or alkylaryl moiety having from 1 to 40 carbon atoms; each of R2, R3 and R4 is independently selected from an optionally-substituted alkylene, alkenylene, alkynylene, arylene, arylalkylene or alkylarylene mo
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Viral Inactivation Process

The invention relates generally to the field of virology. More particularly, the present invention relates to methods for determining the effect of a viral inactivation procedure on the antigenicity of the inactivated virus. In particular for a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, in particular for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). The invention further provides methods to determine the antigenicity of an inactivated viru
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Mycobacterium Mutants for Vaccines with Improved Protective Efficacy

Tuberculosis (TB) is a major health problem and currently, the only licensed TB vaccine is Mycobacterium bovis Bacille Calmette-Guerin (M. bovis BCG). In the present invention, mutation of mycobacterial components reportedly involved in phagosome maturation inhibition was evaluated for vaccine purposes, as such mutations should result in better vaccine antigen processing and presentation. Thus, BCG mutants in genes coding for ManLAM capping a-1,2-mannosyltransferases and the PI3P phosphatase Sap
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L-plastin Antibodies and their Use

The present invention relates to antibodies, especially camelid antibodies, binding to the tandem actin binding domain in L-plastin, but not to the single actin binding domains. It further relates to the use of those antibodies to limit cell invasion and metastasis in cancer.
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Vitamin D3 Analogues for the Prevention and Treatment of Bone Disorders

Novel vitamin D analogues are useful for making pharmaceutical compositions for the prevention or treatment of bone disorders such as osteoporosis
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Method for Alleviating Intestinal Problems and Novel Bacterial Strains Therefor

The present invention relates to butyrate producing bacterial strains related to the species Buty?coccus pullicaecorum to be used in the prevention and/or treatment of intestinal health problems. The present invention therefore provides methods and compositions that overcome the problems associated with the currently used methods for administering butyric acid in the treatment of intestinal health problems in humans and/or animals.
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Blood Serum Marker for Detection and Diagnosis of Alzheimer's Disease

The present invention relates to a blood marker for early detection and diagnosis of Alzheimer's disease. More specifically, it relates to an analysis of the protein glycan profile in the blood, and the identification of specific peak ratio's when compared to healthy aged matched subjects that are typical for Alzheimer's dementia.
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Use of SIP1 As Determinant of Breast Cancer Stemness

The present invention relates to the diagnosis and treatment of cancer. More specifically, it relates to the use of SIP1 nucleic acid and/or protein for the detection of breast cancer stem cells, and the repression of the gene and/or the inactivation of the protein to repress the differentiation of cells into cancer cells and to inhibit metastasis of breast cancer tumors.
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Inhibitors of Malt1 Proteolytic Activity and Uses Thereof

The present invention relates to inhibitors of MALT1 proteolytic and/or autoproteolytic activity. More specifically, it relates to compounds such as, but not limited to peptide derivates such as Z-LSSR-CHO, Z-LSSR-CMK, Z-GASR-CHO, and Z-GASR-CMK,and small compounds such as 5-{[5-(3-chloro-4-methylphenyl)-2-furyl]methylene}-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione and variants thereof, and the use of those compounds for the preparation of a medicament. The invention relates further to a method
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Inhibition of alpha synuclein toxicity

Present inventions demonstrates that alpha synculein toxicity such as [alpha]-synuclein mediated cell death, alpha synuclein induced reactive oxygen species (ROS) in a cell requires the proapoptotic endonuclease G and that the deletion of the endonuclease G or suppressing of the endonuclease G apoptotic pathway attenuates or counteracts such alpha synuclein toxicity. The present invention compositions and methods for inhibition of [alpha]-synuclein toxicity. The inhibiting [alpha]-synuclein toxi
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Methods and Preparations for Protecting Critically Ill Patients

The present invention relates to the use of a polyamine or a salt, solvate, or derivative thereof, such as spermine or spermidine for the treatment or prevention of a life threatening condition, such as multiple organ failure, in a critically ill patient with a non-infectuous disorder.
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Novel Inhibitors of Flavivirus Replication

The present invention relates to a series of novel compounds, methods to prevent or treat viral infections by using the novel compounds, processes for preparation of the compounds, their use to treat or prevent viral infections and their use to manufacture a medicine to treat or prevent viral infections, particularly infections with viruses belonging to the family of the Flaviviridae and more preferably infections with Hepatitis C virus (HCV). The present invention also relates to the novel comp
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Inhibitors of Lentiviral Replication

The present invention provides peptides having lentiviral replication inhibiting properties, more in particular having antiviral activities with respect to HIV (Human Immunodeficiency Virus). The invention further relates to the use of said peptides as a medicine and in the manufacture of a medicament useful for the treatment of subjects suffering from a HIV infection, as well as for treatment of other lentiviral infections and to the treatment of animals suffering from FIV or lentiviral infecti
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Bace1 Inhibitory Antibodies

The present invention relates to antibodies with a specificity for BACE1. More specifically, the invention provides monoclonal antibodies which bind to BACE1 and are capable of inhibiting the activity of BACE1 and methods producing these antibodies. Said antibodies can be used for research and medical applications. Specific applications include the use of BACE1 specific antibodies for the treatment of Alzheimer's disease.
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Thieno [2, 3-B] Pyridine Derivatives As Viral Replication Inhibitors

The present invention relates to a series of compounds of formula (A) having antiviral activity, more specifically HIV (Human Immunodeficiency Virus) replication inhibiting properties. The invention also relates to methods for the preparation of such compounds, as well as to novel intermediates useful in one or more steps of such syntheses. The invention also relates to pharmaceutical compositions comprising an effective amount of such compounds as active ingredients. This invention further rela
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Targeted Radiotherapy

The present invention concerns a therapeutically labeled napthodianthrone or phenanthro[1,10,9,8-opqra]perylene-7,14-dione compound, which comprises a chemical element or an isotope that has an unstable nucleus and emits radiation during its decay to a stable form sufficient to destroy neighboring cells or tissues for use in a targeted radiotherapy to enhance curability of a warm-blooded animal that has been subjected to a necrosis-inducing antitumor therapy. A particular advantage of present in
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Novel Viral Replication Inhibitors

The present invention relates to a series of novel compounds having antiviral activity, more specifically HIV (Human Immunodeficiency Virus) replication inhibiting properties. The invention also relates to methods for the preparation of such compounds, as well as to novel intermediates useful in one or more steps of such syntheses. The invention also relates to pharmaceutical compositions comprising an effective amount of such compounds as active ingredients. This invention further relates to th
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Anti-cancer Combination Therapy

The present invention relates to a combination of therapeutic agents comprising: (a) a cytosine-based anti-cancer drug and/or a purine-based anticancer drug and (b) a therapeutic agent selected from the group consisting of thymidine phosphorylase inhibitors, and antibiotics against Mollicutes bacteria. The present invention also relates to the simultaneous, separate or sequential use of said combination for the treatment of cancer in mammals, especially in humans. The present invention also rela
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Near Full-genome Assay of HCV Drug Resistance

The invention relates to assays for characterization of genotypic mutations of Hepatitis C Virus (HCV) showing a resistance to anti-HCV drugs.
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Inhibition of Tumour Growth and Metastases

The instant invention relates to compounds of formula 1 and their applications as an anti-cancer agent and/or as an angiogenesis inhibitor in mammals, preferably in humans; the instant invention also includes a method of treatment of metastases of prostate cancers using said compound and also a method of treatment of conditions in which angiogenesis must be inhibited. Said compound of formula 1 wherein R1 is a C1-C6 alkyl group, substituted or not, linear or branched and R2 represents a halogen
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Novel Antiviral Agent

The present invention relates the use of a composition including or consisting of at least one element selected from among: a predetermined protein including or consisting of the amino acid sequence SEQ ID NO: 5, and a homologous protein of said predetermined protein, for preparing a drug for preventing or treating diseases related to a viral infection or inflammation.
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Macrocyclic Prodrug Compounds Useful As Therapeutics

The present invention includes macrocyclic prodrug compounds, pharmaceutical compositions containing them. The present invention also includes use of these compounds in the treatment of various diseases including an autoimmune disease, an inflammatory disease, a neurological or neurodegenerative disease, cancer, a cardiovascular disease, allergy, asthma, a hormone-related disease, and tumors or symptoms resulting from neurofibromatosis.
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Anti-aging Compositions Comprising Menyanthes Trifoliata Leaf Extracts and Methods of Use Thereof

An anti-aging composition comprising a skin-beneficial amount of actives identified in Menyanthes trifoliata leaf, wherein the actives are inhibitors of one or more of MMP-1, 2 and 9 and/or scavengers of peroxynitrite. Also disclosed are methods of using such a composition, which include treating the skin for signs of chronological or pre-mature aging.
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Calcium/sodium Salt of Inositol Tripyrophosphate As an Allosteric Effector of Hemoglobin

The present invention relates to mixed calcium/sodium salt of inositol tripyrophosphate, methods of preparing and methods of use. The mixed calcium/sodium salt may be a monocalcium tetrasodium salt of inositol tripyrophosphate. Methods of use include administering the above salts in an effective amount to treat diseases caused by hypoxia or other conditions associated with inadequate function of the lungs or circulatory system, such as various types of cancer and Alzheimer's disease.
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Irreversible Inhibitors Useful for the Treatment of Kinase-related Pathologies

The present invention provides new compounds (I) having a kinase inhibitory activity and useful for treating cancer.
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Synthesis of Resorcylic Acid Lactones Useful As Therapeutic Agents.

Disclosed are macrocyclic compounds of formulae I, I', II, II', III, III', IV, and V, which are analogs of the pochonin resorcylic acid lactones, pharmaceutical compositions comprising the compounds, and methods and uses comprising the compounds for the treatment of diseases mediated by kinases and Heat Shock Protein 90 HSP90.
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Method of Reducing Multi-drug Resistance Using Inositol Tripyrophosphate

Inositol trisphosphate (ITPP) causes normalization of tumor vasculature and is a particularly effective cancer therapy when a second chemotherapeutic agent is administered following partial vascularization. ITPP also treats, alone or in combination, multi-drug resistant cancers. ITPP can also be used to reduce the amount of a second chemotherapeutic drug required for anticancer activity. In addition, ITPP enhances immune response and treats hyperproliferative disorders.
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Methods and Compositions for Treating Cancer

The present invention relates to uses or methods for treating proliferative diseases, in particular cancer, implementing ruthenium compounds, as well as to compositions containing same.
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Calcium/sodium Salt of Inositol Tripyrophosphate As an Allosteric Effector of Hemoglobin

The present invention relates to mixed calcium/sodium salt of inositol tripyrophosphate, methods of preparing and methods of use. The mixed calcium/sodium salt may be a monocalcium tetrasodium salt of inositol tripyrophosphate. Methods of use include administering the above salts in an effective amount to treat diseases caused by hypoxia or other conditions associated with inadequate function of the lungs or circulatory system, such as various types of cancer and Alzheimer's disease.
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Hydroxy-bisphosphonic Acid Derivatives As Vector for Targeting Bone Tissue

The present invention relates to hydroxy-bisphosphonic acid derivatives corresponding to general formula (I): in which: - n and m denote, independently of one another, an integer ranging from 1 to 4, - X denotes an oxygen atom or an N-R3 group, - R1 and R3 denote, independently of one another, a linear or branched C1 to C6 alkyl group, and - R2 denotes a residue of a molecule of therapeutic or diagnostic interest, or pharmaceutically acceptable salts of said derivatives, and also the method for
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Modified phosphocalcic compound, injectable composition containing same

A phosphocalcic compound modified by a gem-bisphosphonic acid or one of its salts, a method for preparing same, as well as its use for preparing an injectable composition. The modified phosphocalcic compound is obtained by adding a gem-bisphosphonic acid or one of its alkali metal or alkaline earth salts to a suspension of a precursor phosphocalcic compound in ultras-pure water, while stirring the reaction medium at room temperature, then in recovering by centrifuging the formed compound. The co
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Injectable Calcium-phosphate Cement Releasing a Bone Resorption Inhibitor

The present invention relates to a macroporous, resorbable and injectable apatitic calcium-phosphate cement with a high compressive strength useful as bone cement and releasing a bone resorption inhibitor, preparation method and uses thereof.
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Macroporous and Highly Resorbable Apatitic Calcium-phosphate Cement

The present invention is directed to a novel cement powder comprising an organic component consisting of one or more biocompatible and bioresorbable polymers and an inorganic component consisting of one or more calcium phosphate compounds. The invention also relates to the apatitic CPC resulting from the mixing of said cement powder with a liquid phase and setting.
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Treatment of Tumours Using T Lymphocyte Preparations

The invention relates to the treatment of a tumour in a patient, by injecting T lymphocytes depleted of regulatory T lymphocytes, and expressing a molecule enabling the specific destruction thereof, wherein the patient receives, beforehand, a non-myeloablative or myeloablative lymphopenia-inducing treatment.
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Method for Optimizing Blood Cell Transplants

The invention relates to the use of allogenic T lymphocytes for the preparation of a composition intended to be injected into a recipient patient as conditioning for a hematopoietic stem cell transplant, said allogenic T lymphocytes expressing a molecule enabling the specific destruction thereof.
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Tumour-cell-fixing Cells

The present invention relates to an isolated cell capable of fixing tumour cells, expressing the CD10 protein and expressing at least one MDR protein, and to the use of this cell for screening for anti-tumour compounds. Figure: none.
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Use of Interfering RNA for Treating an HIV Infection

The present invention relates to interfering RNA, in particular miRNAs capable of specifically blocking the replication of an HIV virus strain resistant to an antiretroviral compound, and to the use thereof for treating infections by said type of virus.
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Compounds for Preventing and Treating Plasmodium Infections

The present invention relates to the use of at least one compound of formula (I) for the manufacture of a medicament intended for preventing or treating infections by a Plasmodium parasite in an individual, by inhibiting the pre-erythrocytic development stage of said Plasmodium parasite.
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Treatment of Mood and Anxiety Disorders

The invention relates to a selective inhibitor of EIk-1 or MSK-1 activation for use in the prevention and/or treatment of mood and anxiety disorders.
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Tyrosine Kinase Receptor Tyro3 As a Therapeutic Target in the Treatment of Cancer

The present invention concerns new methods for treating cancer by using TYRO3 inhibitors and methods for identifying new molecules of interest for treating cancer.
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Bone Substitute, and Method for the Preparation Thereof

The invention relates to a material that can be used as a bone substitute and to a method for the preparation thereof. The material comprises an organic phase (I) comprising striated collagen fibrils constituted of collagen I triple helices, said fibrils being organized over a large distance according to a 3D geometry associating aligned domains and cholesteric domains, and also isotropic domains where they are not organized; and a mineral phase (II) comprising apatite crystals having a hexagona
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Treatment of Retinal Degeneration

The use of a compound having a gon-4-ene nucleus, said nucleus having attached thereto, in the 13 position, a polycarbon-alkyl radical having 2 to about 16 carbon atoms, said compound containing at least 19 and up to 40 carbon atoms in its carbon-carbon skeleton, in the manufacture of a medicament for the treatment or prevention of a disease or condition characterised by apoptosis or degeneration of mammalian cells.
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A Method for the Treatment of Cancer

The invention is based on the surprising finding that treatment with a chemotherapeutic agent such as 5-fluorouracil (5-FU) and an autophagy inducer effectively inhibit the continued growth of, and prevent the recovery following drug withdrawal, of cancer cells. In vivo, drug resistance from a failure to adequately engage in apoptotic programmed cell death leads to a recurrence of cancer and tumours can remain dormant for periods of time before re-emerging as drug resistant metastases. It has be
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Composition and Method for Treatment of Preterm Labor

The invention provides a method for treating or preventing preterm labor in pregnant female subjects. The method comprises the step of administering a therapeutically effective amount of a Toll-like Receptor 9 antagonist sufficient to prevent the activation of Toll-like Receptor 9 by fetal DNA. The invention further provides compositions comprising a Toll-like Receptor 9 antagonist for use in the methods of the invention. Said compositions and methods enhance gestation and therefore improve neon
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Treatment of Infections

The present invention is directed to improved microbial antigen vaccines, pharmaceutical compositions, immunogenic compositions and antibodies and their use in the treatment of microbial infections, particularly those of bacterial origin, including Staphylococcal origin. Ideally, the present invention is directed to a recombinant staphylococcal MSCRAMM or MSCRAMM-like proteins, or fragment thereof, with reduced binding to its host ligand, for use in therapy.
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Compounds and Methods for the Treatment of Autoimmune and Inflammatory Disease

The present invention provides a method and composition for the treatment and prevention of an autoimmune disease such a multiple sclerosis which is mediated by autoreactive T cells. The administration of a NOD-1 agonist is shown to mediate an anti-inflammatory immune response. NOD-1 agonists suitable for use in the methods and compositions of the invention include diaminopimelic acid (DAP)-containing muropeptide compounds such as Tri-DAP and M-TriDAP.
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Targeting Prodrugs and Compositions for the Treatment of Gastrointestinal Diseases

Provided herein are compounds, compositions and methods for preventing or treating gastrointestinal cancer in a mammal, wherein the method comprises delivering an effective amount of a COX-2 or a similar sulfonamide inhibitor as a prodrug or derivative thereof to the colon, wherein the COX-2 or similar inhibitor is released in- vivo.
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Compositions and Methods for Modulating Toll-like Receptor Activity

The present invention relates to compositions and methods for use in the treatment of conditions such as septicaemia and septic shock. The invention further provides compositions and methods for the suppression of Toll-like Receptor 14 interaction with CD14 during Toll-like Receptor mediated signalling. The invention further provides screening assays to identify compounds which have utility in preventing the association of Toll-like Receptor 14 and CD14.
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Treatment of Microbial Infections

The present invention is directed to improved microbial antigen vaccines, pharmaceutical compositions, immunogenic compositions and antibodies and their use in the treatment of microbial infections, particularly those of bacterial origin, including Staphylococcal origin. Ideally, the present invention is directed to a recombinant staphylococcal MSCRAMM or MSCRAMM-like proteins, or fragment thereof, with reduced binding to its host ligand, for use in therapy.
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Method and Apparatus for Stimulating Pelvic Floor Muscles

A method of and apparatus for stimulating pelvic floor muscles in a patient comprises applying at least one electrode externally to each side of the patient's body in the region of the pelvis, and energising the electrodes to apply a muscular stimulation current which flows laterally across the patient through the patient's pelvic floor. Preferably a plurality of electrodes are applied symmetrically on each side of the body. The electrodes may be incorporated in a garment.
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Causal Therapy of Diseases Or Conditions Associated with CNS Or PNS Demyelination

The invention broadly relates to the use of the Active in the causal treatment of a disease caused by axonal demyelination, in which the Active maintains the integrity of myelination (for example by promoting remyelination, and/or preventing demyelination, of the axonal sheaths). The invention is particularly directed to the causal treatment of CNS demyelination diseases, for example MS, especially primary progressive MS and/or relapse remitting MS, and PNS demyelination diseases, for example Ch
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Detection and Treatment of an Invasive Cancer Phenotype

A method for the inhibition, prevention or treatment of invasive/metastatic cancer in an individual in need thereof, comprises a step of treating the individual with an agent capable of attenuating the activity of protein selected from the group consisting of: STIPl; and ALDHlAl. Suitably, the invasive/metastatic cancer is pancreatic cancer, typically a metastases selected from the group consisting of: bone metastases; lung metastases; liver metastases; bone marrow metastases; breast metastases;
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A Method of Assessing Cancer Status in an Individual

The invention relates to a method of screening patients to identify patients at risk of having a lung squamous cell carcinoma. A sample of serum from the individual is assayed for the abundance of a protein selected from the group consisting of: SEQUENCE ID NO'S: 1 to 11 relative for a control abundance for that protein, and the relative abundance obtained is correlated with risk of lung SCC cancer. The diagnosis may involve a single diagnostic variable or a panel of diagnostic variables. For ea
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A Method of Predicting Response to Thalidomide in Multiple Myeloma Patients

A method of predicting response to thalidomide, or thalidomide analogs, in an individual with cancer, especially cancers for which thalidomide has been implicated as a treatment, such as Multiple Myeloma (MM) employs one or more of a panel of biomarkers that have been shown to be differentially expressed in cancer patients that respond to thalidomide (hereafter "Responders") relative to cancer patients that do not respond to thalidomide (hereafter "Non-responders). The method involves assaying a
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HIV-1 Envelope Polypeptides for HIV Vaccine

Immunogenic HIV-1 envelope polypeptides are provided, wherein specificamino acid residues are mutated to repress immunosuppression in GP41, thereby boosting the immune response against HIV-1. Specifically, mutation of those specific residues does not affect the fusogenic propertiesof the viral particleand/or the overall protein structure of the viral envelope protein.The invention further provides peptides and antigens based on the immunogenic HIV-1 envelopes as well as nucleic acid sequences an
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Nanoparticle-mediated Treatment for Inflammatory Diseases

The present invention provides nanoparticles for treatment of inflammatory diseases. The nanoparticles preferably comprise chitosan and a siRNA targeting a mRNA encoding a pro-inflammatory cytokine, such as e.g. tnf-alfa. A preferred route of administration of the nanoparticles is by injection intraperitoneally.
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Method for Early Detection of Cancer

The present inventors have demonstrated that circulating auto-antibodies to cancer antigens hold promise as specific and sensitive biomarkers for the early detection of cancer. The present invention thus relates to methods of detecting cancer in a sample, comprising utilising a glycopeptide bait derived from human mucins with different cancer-associated O-glycans. Detected antibodies were demonstrated as glycopeptide specific, and it could be discriminated between e.g. colorectal cancer patients
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PDZ Domain Modulators

This invention relates to compounds useful as PDZ domain modulators, in particular the PDZ domain of PICK1. In other aspects the invention relates to the use of these compounds in a method for therapy and to pharmaceutical compositions.
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Methods of Treating Thromboembolic Disorders

The field of the invention relates to methods for dissolving a thrombus using inhibitors of platelet contractility. More particularly, the present invention relates to the use of an inhibitor of platelet contractility in combination with one or more thrombolytic agents and optionally one or more anticoagulants for inhibiting platelet contraction and consolidation in the developing thrombus.
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Compositions and Methods for the Treatment of Disease

The present invention relates to pharmaceutical compositions for the treatment and/or prophylaxis of disease associated with fibrosis in a vertebrate, said composition comprising at least one activin antagonist, and optionally a pharmaceutically acceptable carrier, adjuvant and/or diluent. The invention also relates to methods of treatment of disease associated with fibrosis in a vertebrate, as well as methods for diagnosing such conditions, and kits therefor.
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Ectopic Pregnancy Treatment

The invention pertains to methods for treating ectopic pregnancy. More particularly, the present invention relates to methods for treating unruptured ectopic pregnancy using a non-surgical method comprising the administration of an EGFR inhibitor alone or in combination with an anti-metabolite e.g. methotrexate (MTX). The methodology is potentially applicable to treatment of unruptured ectopic pregnancies of all sizes.
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A Method for Treating Obesity

A method and pharmaceutical composition for treating a disease or condition associated with elevated hypothalamic T cell protein tyrosine phosphatase (TCPTP) such as obesity, weight gain, type 2 diabetes mellitus, insulin sensitivity, impaired glucose tolerance, and inflammation is disclosed. The method involves administering a subject with an agent which inhibits the activity or expression of TCPTP, wherein the agent is administered, adapted and/or formulated in a manner ensuring that an effect
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STAT3 Inhibitors for the Treatment of Fibrosis

A method of medical treatment or prevention of an interstitial lung disease (ILD) in a subject in need thereof, the method including administering a therapeutically effective amount of a signal transducer and activator of transcription 3 (STAT3) inhibitor to the subject is provided. In other aspects uses of STAT3 inhibitors for treatment and/or prevention of an ILD, or for preparation of medicaments for treatment or prevention of an ILD are provided. The method or use may further include couplin
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Antisense Molecules and Methods for Treating Pathologies

An antisense molecule capable of binding to a selected target site to induce exon skipping in the dystrophin gene, as set forth in SEQ ID NO: 1 to 59.
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Method for Preventing Transport of a Neurotropic Virus

The present invention relates to viruses, particularly to cellular transport of neurotropic viruses and the viral and cellular proteins involved with cellular transport of neurotropic viruses.
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Use of Vitamin D Receptor Agonists and Precursors to Treat Fibrosis

This application relates to methods of treating, preventing, and ameliorating fibrosis, such as fibrosis of the liver, kidney, or pancreas. In particular, the application relates to methods of using a vitamin D receptor agonist (such as vitamin D, vitamin D analogs, vitamin D precursors, and vitamin D receptor agonists precursors) for the treatment of fibrosis. Also disclosed are methods for screening for agents that treat, prevent, and ameliorate fibrosis.
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Detection of Anti-parasitic Compounds

The invention relates to screening compounds for anti-parasitic activity, especially for activity against parasitic apicomplexans using Chromerida.
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TREATMENTS FOR EXCITOTOXICITY AND Ass-MEDIATED TOXICITY AND AGE-ASSOCIATED NEURONAL DYSFUNCTION

The invention relates to compounds for preventing, inhibiting or antagonising fyn from phosphorylating an amino acid residue in a molecule having an amino acid sequence shown in SEQ ID NO: 1. Typically the compound is capable of binding to fyn, to prevent fyn from migrating to a region of a nerve cell cytosol adjacent a post synaptic membrane and in one embodiment the compound holds or otherwise sequesters fyn within the nerve cell soma. The invention also relates to the treatment or prevention
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Novel Metabolic Disease Therapy

The invention relates to the prevention and treatment of metabolic abnormalities characterized by abnormal glucose metabolism, including diabetes mellitus and new onset diabetes mellitus through the use of fibroblast activation protein (FAP) selective inhibitors.
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Method of Determining Response to Treatment with Immunomodulatory Composition

The present invention provides a method for accurately determining the likelihood that a subject will respond to treatment with an immunomodulatory composition comprising detecting one or more markers in a sample from the subject, wherein at least one markers is linked to a single nucleotide polymorphism (SNP) set forth in Table 1 or 3-5, and processes for selecting suitable subjects for therapy or for continued therapy, and for providing appropriate therapy to subjects, based on the assay resul
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Translocator Protein Ligands

The present invention relates to compounds and methods for imaging translocator protein (18 kDa) (TSPO) expression in a subject. This invention also relates to compounds and methods for the treatment of neurodegenerative disorders, inflammation or anxiety in a subject.
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A Method of Delivering Functional Agents Across the Blood-brain Barrier

The present invention provides a method of delivering a functional agent across the blood brain barrier so that the functional agent is delivered into the brain of the subject, the method comprising the step of administering a polymeric capsule associated with said functional agent to the subject whereby the polymeric capsule crosses the blood brain barrier and wherein the polymeric capsule is from 300 nm to 2000 nm in diameter and comprises a polymeric shell defining an internal cavity. Further
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Method of treatment and Agents useful for same

The present invention relates generally to a method for the treatment and prophylaxis of inflammatory conditions. The present invention is predicated in part on the identification of cells of the monocyte/macrophage lineage being critical for inflammation and, in particular, chronic inflammation. In accordance with the present invention, it is proposed that the reduction in levels of monocyte/macrophage-type cells and/or a reduction in the production of inflammatory and pro-inflammatory mediator
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Treatment of Chronic Inflammatory Conditions

A method for the treatment of a chronic inflammatory condition in a patient which comprises administration to the patient of an agent which blocks or inhibits IL-3 signalling events in the patient.
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Antigenic complex for the diagnosis and treatment of porphyromonas gingivalis infection

Disclosed is a purified multimeric complex from P. gingivalis, the complex comprising at least one domain from each of RgpA, Kgp and HagA, and having a molecular weight greater than about 300 kDa. Also disclosed are methods for obtaining the antigenic complex and its use to treat Porphyromonas gingivalis.
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Viral Polypeptides and Methods

The invention is directed to polypeptides and polypeptide fragments from HIV- 1 envelope (Env) proteins following the characterisation of Env structures from environments where HIV isolates expose conserved neutralisation- sensitive Env structures. There is provided a polypeptide being all or a fragment of an HIV-1 envelope protein from a transmission strain of HIV-1, the polypeptide having neutralisation sensitive epitopes that are accessible.
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Pain treatment

The present invention relates generally to a method for the treatment and prophylaxis of pain. In accordance with the present invention, it is proposed that antagonists of GM-CSF are effective in the treatment of pain. Antagonists of GM-CSF include, but are not limited to, antibodies which are specific for GM-CSF or the GM-CSF receptor. The present invention further provides transgenic animals, such as a GM-CSF knock-out mouse, useful for testing antagonists in certain disease models.
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Osteoarthritis treatment

The present invention relates generally to a method for the treatment and/or prophylaxis of osteoarthritis (OA). In accordance with the present invention, an antagonist of GM-CSF can be effective in the treatment of osteoarthritis. An antagonist of GM-CSF includes, but is not limited to, an antibody that is specific for GM-CSF or the GM-CSF receptor. The present invention further provides transgenic animals, such as a GM-CSF knock-out mouse, useful for testing antagonists in certain disease mode
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Treatment of Fibrosis

The present invention relates to methods and compositions for treating fibrosis. In one aspect the invention provides the use of an agent capable of increasing the number of stem cells and/or progenitor cells available to and/or engraftment at a site of fibrosis in the manufacture of a medicament for use in conjunction with an anti- fibrotic agent for the treatment of fibrosis. The anti-fibrotic agent can be supplied to the subject before, at the same time, or after the agent capable of increasi
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Tissue Culture Method Using Magnetically Generated Mechanical Stress

PROBLEM TO BE SOLVED: To provide a culturing method of forming connective tissues such as bone, cartilage, ligament and tendon as tissues functioning like mechanical response, and to provide a culturing device. ; SOLUTION: A method for mechanically stimulating tissue forming cells so as to be applied to tissue engineering and regeneration by exposing cells in mechanical stress generated by a magnetic body generating force in response to applied magnetic field and transmitting the force to the ce
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DEVICES AND METHODS FOR DETECTING ss-HAEMATIN AND HAEMOZOIN

In the application, the change in the magnetic state of the haemoglobin caused by the malarial infection is exploited by detecting suitable properties of haemozoin which are dependent on the application of a magnetic field. Figure 1 shows apparatus, shown generally at (10), for performing magneto-optical detection using photo-acoustic techniques. The apparatus (10) comprises a light source (12), producing a beam of optical radiation (14) which passes through a polariser (16), a variable LC retar
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Treatment of Inflammatory Disorders

The present disclosure relates to the treatment of inflammatory disorders. In particular, although not exclusively the present disclosure relates to agents which inhibit a Mas related gene (Mrg) receptor for use in the treatment or prevention of a disorder associated with inflammation. Also included in the present disclosure are methods of treatment, assays and other subject matter.
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Material Comprising Nm23 Linked to a Substrate for Use in Treating Neuron Disorders

Provided are materials for promoting neuron growth, the materials comprising Nm23 protein, or a biologically effective fragment or derivative thereof, linked to a substrate capable of supporting neuron growth. The Nm23 may preferably be immobilised on the substrate. The materials provided may be suitable for implantation at a site of neuronal disruption. The materials may be adapted to guide neuron growth. The invention also provides an Nm23 protein, or a therapeutically effective fragment or de
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Method for Diagnosing and Treating Muscular Dystrophy

The invention describes a method for identifying an individual exhibiting symptoms of, or having a propensity to develop muscular dystrophy comprising determining the level of expression in a tissue sample from the individual of one or more proteins selected from pax-7, caveolin-3 and/or fast-myosin. The use of caveolin-3 to treat muscular dystrophy and compositions containing the compound are also claimed.
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Use of VAP-1 Inhibitors for Treating Fibrotic Conditions

The present invention relates to inhibitors of VAP-1 and their use as medicaments in treating fibrotic conditions. Furthermore, the present invention relates to a method of diagnosing a fibrotic condition on the basis of elevated level of soluble VAP-1 or SSAO activity in a bodily fluid, and to a kit for use in said diagnostic method.
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Methods of Treatment Using Modified Plasminogen Activator Inhibitor Type-1 Molecules

The present invention generally relates to methods of treating bleeding diseases and disorders and to promote wound healing. The methods comprises administering a procoagulant and a modified plasminogen activator inhibitor type-1 (PAI-1) molecule that displays an increased in vivo half-life of the active form of the PAI-1 molecule. The invention also relates to methods of treating respiratory diseases using a modified PAI-1 molecule.
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Compositions and Methods for Perioperative Bladder Instillation

Polyphenol- and especially catechin-containing compositions (e.g., Polyphenon E) are instilled in the bladder of a subject that previously underwent surgical resection or removal of bladder cancer to reduce cancer growth and/or recurrence.
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Muscarinic Agonists for Neurological Disorders and Methods of Making the Same

Muscarinic receptors with agonist activity and methods of making the same are disclosed. The compound have a thiadiazole compound having a having a quinuclidine ring, a tetrahydropyridine ring, an azobicyclo-heptane ring; or, an azobicyclo-octane ring, wherein a linking group replaces an alkyl sulfonate moiety of the thiadiazole compound.
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Na/K-ATPase Expression As an Indicator for the Treatment of Cancer

Methods for regulating the expression of Na/K-ATPase and uses thereof, including uses in the diagnosis/prognosis and treatment of cancer, are disclosed.
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System for Concurrent Delivery of Thermobrachytherapy in the Treatment of Cancers

A system combines hyperthermia and radiation treatments in a single treatment modality by using a radioactive seed having magnetic properties.
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A PEG-albumin Composition Having At Least One Protected Thiol Region As a Platform for Medications

This composition comprises a polyethylene glycol-albumin composition having at least one protected thiol region wherein the composition comprises PEG-AlbCys34 with Cys-34 preserved as a thiol. The albumin is linked to medications such as, antioxidants with a reduced sulfhydryl group through the Cys 34 residing on the albumin. The compositions are useful in treating patients.
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Soluble Guanylyl Cyclase A1 and A-8r Peptide As Diagnostic Markers of and Therapeutic Targets for Prostate Cancer

The present invention provides materials and methods useful to treat various sGCal- expressing cancers. Materials include peptides which interfere with sGCal's pro-survival functions, thereby resulting in apoptosis of sGCal -expressing cells. In addition, the present invention provides screening assays, diagnostic assays, methods to prognose, methods to treat, and kits.
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Non-invasive Ocular Analyte Sensing System

A noninvasive method and apparatus for determining analyte concentration (e.g., glucose) in a subject that includes measuring light refraction from at least a portion one or more structures. One example of such structure is the subject's iris.
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Peptide Manipulators of Regulatory T Cell Activity

The invention relates to peptide inhibitors of Treg cells. Peptides according to the invention are derived from the FOXP3 protein and inhibit its interaction with NFAT. Such peptides are effective to block the interaction between FOXP3 and NFAT and cause apoptosis in mature regulatory T cells. Such peptides show promising potential for manipulating immune responses in man.
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Anti-inflammatory and Wound Healing Effects of Lymphoid Thymosin B-4

The invention relates to a method of treating inflammatory conditions in a subject comprising administering to a subject a composition comprising a lymphoid thymosin-beta4 polypeptide or a functional lymphoid thymosin-beta4 polypeptide variant. The invention also provides a method of promoting wound healing in a subject comprising administering to the subject a composition comprising a lymphoid thymosin-beta4 polypeptide or a functional lymphoid thymosin-beta4 polypeptide variant. The invention
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Male Contraceptive

A compound having formula I. R1, R2, R3 and R4 are independently H or lower alkyl. R5 is aryl, heteroaryl, cycloalkyl, heterocycloalkyl, aryloxy, heteroaryloxy, cycloalkyloxy, heterocycloalkyloxy, arylamino, heteroarylamino, cycloalkylamino, heterocycloalkyl amino, arylthio, heteroarylthio, cycloalkylthio, heterocycloalkylthio, or cyclic olefin, any of which may be substituted or non-substituted; or substituted alkyl, substituted alkyl oxy or substituted alkyl amino wherein the substituent is an
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Compositions for Use in Cardioplegia Comprising Esmolol and Adenosine

The invention related to a composition for use in cardioplegia, said composition comprising (i) esmolol; and (ii) adenosine, wherein in use the concentration of said esmolol is in the range 0.3-1.5mM, and wherein in use the concentration of said adenosine is in the range 0.1-1.5mM. The invention also relates to methods of making and using such compositions.
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Antithrombotic Compounds

A soluble compound for preventing or reducing blood coagulation comprising an antithrombotic agent and a membrane binding element, wherein the antithrombotic agent has a weight of less than about 5,000 daltons. Also disclosed is a soluble compound for preventing or reducing blood coagulation comprising an anticoagulant joined to a membrane binding element via a joining element, wherein the joining element between the anticoagulant and the membrane binding element is less than about 10,000 dalton
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Treatment of Central Nervous System Damage

The invention provides a method of promoting neuronal plasticity in the CNS of a mammal, the method comprising administering to the CNS of the mammal an agent that reduces the inhibitory properties of chondroitin sulphate proteoglycans. Preferred agents are chondroitinases and sulfatases, e.g. chondroitinase ABC. Also provided are methods of identifying further agents.
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Drug Delivery System Comprising Matrix Metalloproteinase Inhibitors

A composition for topical application to a subjects skin comprising a matrix metalloproteinase inhibitor, more particularly an inhibitor selected from the group consisting of : TIMP-I, TIMP-2, TIMP-3, TIMP-4, Ilomastat, MMP inhibitor 111, CL82198, GM1489, and FN-439 is used for the prevention or treatment of soft skeletal tissue extracellular matrix degeneration, more particularly wherein the soft skeletal tissue is a tendon or ligament, and more particularly where the tendon is -selected from t
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Antibodies Specific for Misfolded Proteins and Methods for their Production

Methods are provided for producing an antibody specific for a target antigen, in particular, antibodies capable of binding to prion proteins. The methods include adding a target antigen stabilising agent to a sample containing a target antigen, allowing the stabilising agent to stabilise the target antigen, incubating the stabilised target antigen with a conventional antibody immobilised on a solid support to form a complex, wherein the immobilised antibody is specific for the target antigen, im
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Carrier

Carriers are provided for transport of a substance across the blood-brain barrier. The carriers comprise an antibody specific for a target antigen, in particular, antibodies capable of binding to prion proteins. The antibodies are produced via methods which include adding a target antigen stabilising agent to a sample containing a target antigen, allowing the stabilising agent to stabilise the target antigen, incubating the stabilised target antigen with a conventional antibody immobilised on a
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Methods and Compositions

A method of inducing relaxation of the cervix of a female mammal, the method comprising administering at least one pharmaceutical agent selected from follicle stimulating hormone or a beta-subunit thereof, luteinizing hormone or a beta- subunit thereof, thyroid stimulating hormone or a beta-subunit thereof, and chorionic gonadotrophin or a beta-subunit thereof, topically to the cervix of the mammal. The method can be used to facilitate artificial insemination of the female mammal. Also provided
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Method and Apparatus for Identifying the Viability of Ischemic Myocardium of a Patient

A method for identifying the viability of is chemic myocardium of a patient. The method includes the steps of measuring in real-time the is chemic myocardium of a beating or non-beating heart, and determining in real-time whether the is chemic myocardium of the beating or non-beating heart is stunned or is nonviable. An apparatus for identifying the viability of is chemic myocardium of a patient's heart is further disclosed, including an electrode array having at least four electrodes for electr
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System, software, and method for detection of sleep-disordered breathing using an electrocardiogram

A system to form and store an electrocardiogram (ECG) signal derived from a cardiac electrical signal that includes an apparatus having a pair of the electrodes to connect to a patient to detect the cardiac electrical signal. A signal sampler samples the cardiac electrical signal to form the ECG signal. A data storage device stores the ECG signal. A computer communicates with the data storage device to retrieve the ECG signal for analysis by software stored in the memory of the computer. The sof
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Dry Powder Drug Delivery Formulations, Methods of Use, and Devices Therefore

The present disclosure relates to systems, methods, and formulations for the pulmonary administration of one or more therapeutic agents, in dry powder form, in a single, large dose quantity. These formulations, methods, and systems are useful in the treatment of patients suffering from toxic or harmful gas exposure, such as nerve gas exposure, as well as in the treatment of patients suffering from diseases of the pulmonary system, including tuberculosis, cystic fibrosis, and chronic obstructive
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Hollow Gold Nanospheres (Haunss) and Haunss-loaded Microspheres Useful in Drug Delivery

A near-infrared mediated drug delivery system comprising a plurality of microspheres made of polymeric material, each sphere containing a plurality of hollow gold nanospheres together with drug product, wherein upon NIR irradiation, the drug product is released from the microsphere.
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Combinational Multidomain Mesoporous Chips and a Method for Fractionation, Stabilization, and Storage of Biomolecules

A new fractionation device shows desirable features for exploratory screening and biomarker discovery. The constituent MSCs may be tailored for desired pore sizes and surface properties and for the sequestration and enrichment of extremely low abundant protein and peptides in desired ranges of the mass/charge spectrum. The MSCs are effective in yielding reproducible extracts from complex biological samples as small as 10 III in a time as short as 30 minutes. The MSC-based device may serve as a d
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Use of Riboflavin in the Treatment of Hypertension.

The invention provides the use of riboflavin in the manufacture of a medicament for the treatment or prophylaxis of elevated blood pressure in a subject homozygous or heterozygous for the MTHFR C677T polymorphism. The invention also provides a pharmaceutical product for the treatment or prophylaxis of elevated blood pressure in a subject homozygous or heterozygous for the MTHFR C677T polymorphism, comprising a pharmaceutically effective amount of an anti-hypertensive agent and riboflavin, and th
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Method of Stratifying Breast Cancer Patients Based on Gene Expression

The present invention assists in prospectively predicting the metastatic likelihood, and thereby, the likely clinical outcome of breast cancer patients, based on the genotype of the patient, in particular, by determining the relative expression level of a set of genes, or subsets thereof. The present invention provides use of an expression level of a gene set for the identification of animals, optionally patients, likely to progress to an invasive phenotype, the gene set comprising at least some
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Methods for Reducing Meiotic Non-disjunction

The present invention relates generally to methods for reducing chromosome non-disjunction and aneuploidy during meiosis in mammals by administering to a gamete an effective amount of cdh1, a derivative, variant or homologue thereof, or an agent capable of increasing the expression or production of cdh1. The method may be used for increasing pregnancy rates resulting from assisted reproductive technologies by administering such an agent to the gamete of a female mammal undergoing assisted reprod
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Methods of Inhibiting Fibrogenesis and Treating Fibrotic Disease

The present invention relates to the discovery of an epigenetic relay pathway that controls hepatic stellate cell activation and the wound-healing response in fibrogenesis, including fibrogenesis of the injured liver. Methods of inhibiting fibrogenesis, including liver fibrogenesis and secondary disease states and conditions thereof, and in treating liver damage, including cirrhosis of the liver (which may be caused by viruses or chemicals, including alcohol), are aspects of the present inventio
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The Use of Lipid Emulsions for Binding Hepatitus C Virus

The present invention relates to compositions, methods and apparatus for use in the treatment of Hepatitis C Virus infection. The methods and apparatus are all based upon the use of very low density lipid particles which comprise a triglyceride core surrounded by a phospholipid layer that have been found by the inventors to bind to Hepatitis C Virus particles.
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Method of Inhibiting Clostridium Difficile By Administration of Oritavancin

Glycopeptide antibiotics, such as oritavancin, demonstrate significant activity against both a vegetative form of C. difficile and C. difficile spores. Methods for the treatment, prophylaxis and prevention of C. difficile infection and disease in animals, including humans, are described.
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Compositions and Methods for Reducing Macrovascular Complications in Diabetic Patients

The present invention relates to compositions and methods for reducing or ameliorating macrovascular complications in diabetic patients using C-peptide. The compositions and methods of the present invention are for use in controlling or reducing intimal hyperplasia and for reducing the risk of graft failure in diabetic patients undergoing coronary artery bypass surgery in addition to ameliorating primary atherosclerotic disease in diabetic patients. The invention also includes the use of C-pepti
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Methods and Compositions for Treating Leukemia

A combination of a BCR-ABL inhibitor and a hedgehog pathway inhibitor for the treatment of leukemia.
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Medical Instrument for Grasping an Object, in Particular Needle Holder

A medical instrument for grasping an object, in particular a surgical needle holder, including an elongated shaft, two jaw parts arranged at a distal end of the shaft, at least one of the jaw parts being pivotable with respect to the other of the jaw parts between a grasp state for grasping the object between the jaw parts and a release state for releasing the object. A handle is arranged at a proximal end of the shaft and has at least one operating element for moving the at least one pivotable
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Treatment for keratinizing dermatological disorders by reduction in keratin expression

Compounds and a method for screening for a compound to reduce the expression of keratin proteins in keratinizing dermatological disorders are disclosed. Preferably keratin expression is reduced by the upstream targeting of the transcription factor stat1 using statins (HMG CoA inhibitors), such as Compactin, Mevastatin, Simvastatin, Atorvastatin, Cerivastatin, Fluvastatin, Lovastatin, Pitavastatin, Pravastatin or Rosuvastatin. Alternatively keratin expression may be reduced using RNAi strategies.
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Modulation of Rsk

The present invention relates to specific compounds for use in controlling inflammatory cell function and their potential use in modulating an immune response or inflammation. The present invention also relates to the identification of an alternative RSK pathway in, for example, dendritic cells and the development of assays for the identification of compounds capable of modulating the alternative RSK associated pathway in dendritic cells/macrophage cells and their potential use in enhancing, or
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Sigma Ligands and IKK / NF - KB Inhibitors for Medical Treatment

The present invention relates to agents that act in concert with sigma receptor ligands to enhance their effectiveness in the treatment of cancer and inflammatory disease. It also relates to methods whereby agents can be identified that will be of use in combination with sigma ligands in the treatment of the aforesaid diseases.
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Protein Product for Modifying Cardiovascular Health

A composition for use in the treatment or prophylaxis of conditions mediated by endothelial function in mammals is provided. The composition includes a milk protein hydrolysate generated by treating milk or milk whey protein with a proteolytic enzyme having subtilisin or subtilisin-like activity and/or glutamyl endopeptidase or glutamyl endopeptidase-like activity. The composition is useful in the management of vascular conditions. The invention relates to a method for beneficially modifying
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Diagnosis and Treatment of Cell Proliferation and Differentiation Disorders Based on the FMN2 Gene

The present invention provides methods for diagnosing cell proliferation and/or differentiation disorders, compounds and methods for treating the same and methods for identifying agents potentially useful in the treatment of cell proliferation and/or differentiation disorders.
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Treatment Or Prophylaxis of Proliferative Conditions

The disclosure relates to novel compounds for use in the treatment or prophylaxis of cancers and other proliferative conditions that are for example characterized by cells that express cytochrome P450 1B1 (CYP1B1) and allelic variants thereof. Also provided are pharmaceutical compositions comprising one or more such compounds for use in medical therapy, for example in the treatment of prophylaxis of cancers or other proliferative conditions, as well as methods for treating cancers or other condi
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Angina Treatment

A xanthine oxidase inhibitor for use in the treatment of angina is described. The angina may be stable angina pectoris and the treatment may be to eliminate, reduce or alleviate one or more symptoms of angina pectoris (such as chest pain), or may be prophylactic. The xanthine oxidase inhibitor may be used in combination with one or more additional therapeutic agents, such as anti-anginal agents. Pharmaceutical compositions, medicaments and kits for the treatment of angina are also described.
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Methods

The invention relates to a method of detecting an altered behaviour in a population of cells, said method comprising determining at least one of the following characteristics of the population of cells; (i) the proportion of stem cells, proliferating cells and differentiated cells in said cell population; or (ii) the size of stem cell clusters in said cell population; or (iii) the separation of stem cell clusters in said cell population; and comparing said at least one characteristic to a refere
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Mannosylated Butyrophilin Tumour Markers

This invention relates to the finding that butyrophilins (BTNs) are mannosylated when expressed by cancer cells. Methods and compositions relating to the detection and treatment of cancer cells expressing mannosylated butyrophilins (BTNs) are provided.
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Formulations of Viable Cells for Oral Delivery

This invention relates to solid formulations for the oral delivery of live microbial cells which comprise dried viable cells and small amounts of a bile acid binding agent, for example, an anion exchange resin such as cholestyramine. The presence of bile acid binding agents in the formulation significantly increases the survival of the cells in the intestinal tract and facilitates delivery of the viable cells to the intestine.
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Methods for Predicting Autoimmune Disease Risk

The invention relates to means and methods for determining whether a subject is at high or low risk of autoimmune disease progression by determining the CD8 or CD4 cell subtype of the subject. Autoimmune diseases of particular interest include vasculitis, systemic lupus erythematosus (SLE), rheumatoid arthritis, multiple sclerosis, and inflammatory bowel disease. The invention also relates to means and methods for determining the CD8 or CD4 cell subtype of a subject, e.g. for predicting response
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Treatment of Diseases Related to Hyperactivity of the Complement System

Raising the level of Factor I above physiological levels can be used to treat diseases in which the underlying pathology is linked to overactivity of the C3b-feedback cycle and the generation and pro-inflammatory effects of iC3b. Methods, agents, and compositions for treatment of such diseases are described.
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Modulation of T-cell Mediated Immune Responses

This invention relates to the finding that the binding of SKAPl to RapL plays a role in the LFA-I mediated adhesion of T cells. Methods are provided for the identification of compounds which alter this binding and which may therefore be useful in the modulation of T-cell immune responses.
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Methods for Treating Inflammatory Arthritis

Provided herein are, inter alia, methods of treating subjects suffering from inflammatory arthritis and identifying candidate compounds for treating inflammatory arthritis.
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Visual Perimeter Measurement System and Method

In one example of the invention a patient display is provided on which a fixation graphic or video is shown in substantially the centre of the display, and then target graphics or video are shown at different positions on the display about the centre. A test subject is positioned in front of the patient display a known distance therefrom, and a camera captures an image of the test subject's eyes, to allow gaze direction determination to be performed. The captured images are displayed to a clinic
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Treatment of Pre-term Neonates

This invention relates to the treatment of pre-term neonates with paraoxonase 3 (PON3) or serum amyloid A like protein 3 (SAA3) to reduce or prevent the morbidity and mortality associated with prematurity.
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Composition

The present invention relates to a composition for improving stem cell integration. The present invention also relates to a method of improving stem cell integration and kits for improving stem cell integration.
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Neural Interface

This invention relates to the neural interface comprising one or more electrode-coupled microchannels. Each electrode-coupled microchannel comprises a microchannel for accommodating regenerating nerve axons; and one or more electrodes exposed to the interior of said microchannel. The one or more electrodes may comprise (i) a stimulation array suitable for generating an extracellular stimulus current which induces an action potential in an axon in the microchannel; and/or (ii) a recording array w
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Improved Reprogramming of Mammalian Cells, and Cells Obtained

Expression of reprogramming factors such as Sox2, klf4, c-myc, Nanog, LIN28 and Oct4 followed by culture in a MEK inhibitor and a GSK3 inhibitor reprograms tissue cells. The invention provides new uses of these inhibitors, for example in inducing completion of the transcriptional resetting of so-called pre-pluripotent (pre-iPS) stem cells, for example as obtained from mammalian neural stem cells or epiblast stem cells treated with single or combinations of the reprogramming factors, expressed tr
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Methods of Using Anti-inflammatory Compounds

The invention relates to the use of 3-(2',2'-dimethylpropanoylamino)-tetrahydropyridin-2-one for preparing a medicament intended to prevent or treat inflammatory disorders.
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Treatment of central nervous system damage

The invention provides a method of promoting neuronal plasticity in the CNS of a mammal, the method comprising administering to the CNS of the mammal an agent that reduces the inhibitory properties of chondroitin sulphate proteoglycans. Preferred agents are chondroitinases and sulfatases, e.g. chondroitinase ABC. Also provided are methods of identifying further agents.
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SCD Fingerprints

This invention relates to methods of testing, diagnosing, monitoring, prognostically stratifying and classifying disease, disorders and other medical conditions and physiological states through the detection and analysis of soluble CD antigens in a body fluid sample.
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Processing retinal images using mask data from reference images

Generating output data indicating areas of a retinal image that represent lesions comprises, receiving candidate date associated with a retinal image indicating areas of the image representing candidate lesions; receiving mask data indicating areas of the image that do not represent lesions, the mask data may be produced using a reference image; and processing the candidate data and mask data to generate output data indicating areas of a retinal image representing lesions.
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Handling blood or plasma by EM radiation - in wavelength region of 200 to 320 nanometres at radiation intensity of 1 joule to 1 kilo-joule per sq. metre

The blood or plasma is irradiated directly after and/or before the material exchange process (haemo-dialysis or plasmapheresis) without direct contact through the conveying system (1). The inner surface of the material transfer system is subjected to a radiation intensity of 1J to 1KJ/sq.m. The start and stop of the radiation are set by a timer circuit. The whole radiation regime is carried out by a microcomputer (8). A hemispherical reflector (3) is fixed to a holder for an u.v. radiator with f
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Filter for selective leucocyte removal from blood etc. - has packing material of plant cell walls, not altering concn., function etc. of erythrocytes

Filter for selective elimination of leucocytes from blood, blood preparations or other leucocyte-contg. biological liq. comprises a sieve-like filter base and as column packing an adsorbent made from a framework of plant cell-walls. USE/ADVANTAGE - The method is esp. useful in transition medicine and haematology (removal of leucocytes avoids problems of non-haemolytic febrile reactions). It is simple and economical and the compsn. of the cellular and acellular blood components is not changed by
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Polymerase-inhibiting virustatic agent - extracted from root of Cochlospermum angolense as carotenoid, effective against HIV reverse transcriptase, influenza replicase, etc.

The inhibitor contains a compound (I) from the root of Cochlospermum angolense, with appropriate carriers and adjuvants. (I) is a carotinoid drug extract, obtained with water or alcohol from the finely powdered root. The extract is used in crystalline or amorphous form in tablets or capsules, or as drops. Dose is 100-200 micro-g/ml per unit, corresponding to 100-200 mg/kg body weight per day. USE/ADVANTAGE - The inhibitor is useful in the treatment of virus infections. It inhibits HIV reverse tr
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Antiprotozoal compsns. - contain root extract of Cochlospermum angolense

Antiportozoal compsns. contain an active ingredient derived from the roots of Cochlospermum angolense. USE/ADVANTAGE - The compsns. are active against a wide range of human- and animal-parasitic protozoa, including Plasmodium, Naegleria, Giardia, Trichomonas, Leishmania and Crithidia spp..
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Anthelmintic agent to treat diseases due to worms - contg. carotenoid drug extracted from roots of cochlospermum angolense

Antihelminth agent contains pharmacologically effective doses of the root contents of Cochlospermum angolense and conventional carriers and auxiliaries. Active ingredient is pref. a carotinoid drug extract form the roots of Cochlospermum angolense, esp. in crystallised or amorphic form (in which case the agent is in the form of tablets of capsules) or an aq. or alcoholic extract (in which case the agent is pref. in the form of drops). Pref. the carotinoid root extract is in max. amts. of 100-200
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Procedures and cutlery for the digestion of cells and the preservation and extraction of nucleic acids from living cells or tissues

The invention relates to a method for sample fixation, sample preservation, for cell disruption and extraction of nucleic acids in living cells, cell aggregates and tissue samples, and a working set for the implementation of the procedure. The method is to use living cells or tissue fragments of a nearly water-free to equilibrate with water-miscible volatile organic liquid and catch up then, the dehydrated cells through generation of mechanical stimuli in the volatile organic liquid to separate
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Fluorescent probe comprises two complementary hybridization sequences that are separated by a peptide with a recognition sequence for a target molecule, which also separates a donor chromophore from a

Fluorescent probe comprises two complementary hybridization sequences that are separated by a peptide with a recognition sequence for a target molecule, which also separates a donor chromophore from an acceptor chromophore. The length of the peptide is such that the hybridization sequences can hybridize with each other. Independent claims are also included for: (1) array on which probes as above are immobilized; (2) kit for detecting a target molecule in vitro, comprising a probe as above, a rea
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Podophyllotoxin

PROBLEM TO BE SOLVED: To provide a prodrug of podophyllotoxin having sufficiently reduced adverse effects by the administration to a patient. ; SOLUTION: There is provided a compound in which the prodrug and the like of the podophyllotoxin are bound. In chemical formula (IV), R<SB>1</SB>and R<SB>2</SB>are each 1-10C alkyl; or R<SB>1</SB>and R<SB>2</SB>, together with carbon to which they are bound, represents 5-6C cycloalkyl; or R<SB>1</SB>is H; and R<SB>2</SB>is selected from the group consisti
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Material layer and electro-surgery system for electrosurgical tissue fusion...

The invention relates to a biocompatible material layer for use as an intermediate layer between tissue surfaces to be fused with a fusion of body tissue. The layer of material is such that it causes the body to be fused between tissue surfaces areas of relatively higher electrical resistance and areas of relatively lower electrical resistance.
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Methods for Producing Hair Microfollicles and De Novo Papillae and their Use for in Vitro Tests and in Vivo Implantations

The present invention relates to a method for producing hair microfollicles comprising the steps of: a) providing de novopapillae, b) providing other cell populations selected from the group of fibroblasts, keratinocytes and melanocytes, and co-culturing the de novopapillae with at least one other cell population in non-adherent culture vessels.The present invention relates also to methods of producing de novo papillae usable in said method for producing hair microfollicles.
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Combined Use of TGF-Beta Signaling Inhibitor and Antitumor Agent

The combined use of a TGF-beta signaling inhibitor and an antitumor active substance or an imaging agent modified by a drug-encapsulating macromolecular micelle, or the like, is provided. The selective delivery capability of the antitumor active substance or the imaging agent to the target is improved, increasing the antitumoral activity in the target. The present invention relates to use of transforming growth factor [beta] (TGF-[beta]) signaling inhibitor, for increasing leakiness neovascul
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Contrast Medium-containing Organic-inorganic Hybrid Particle

PROBLEM TO BE SOLVED: To provide a carrier for stably delivering an MRI contrast medium to a target site. ; SOLUTION: The organic-inorganic hybrid particle comprises a block copolymer containing an unchargeable hydrophilic polymer chain segment and a polymer chain segment containing a repeating unit having a carboxylate ion group on a side chain, a calcium ion (Ca<SP>2+</SP>), a phosphate ion (PO<SB>4</SB><SP>3-</SP>) or a carbonate ion (CO<SB>3</SB><SP>2-</SP>) or a mixture of these anions, and
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Polyion Complex Comprising PHD2 Expression Inhibiting Substance

Disclosed is a safer and more sustainable gene delivery system or the like, which is effective for treatment of ischemic diseases and the like. Specifically disclosed is a pharmaceutical composition which contains, as an active ingredient, a polyanionic substance that inhibits the expression of PHD2, and which contains a polyion complex of the polyanionic substance and a polycationically charged polymer.
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Polymer/metal Complex Composite Having Mri Contrast Ability and Mri Contrasting And/or Antitumor Composition Using the Same

The present invention provides a polymer-metal complex composite, which comprises a block copolymer capable of serving as a constituent member of a polymeric micelle and a metal complex having MRI contrast ability, accumulates in a tumor-specific manner, achieves high image contrast even in a small amount, and has reduced side effects and a long retention time in blood. The polymer-metal complex composite of the present invention comprises a block copolymer (A) represented by general formula (a)
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Compositions for Cancer Prevention, Treatment, or Amelioration Comprising Papaya Extract

The components of a brew/extract of leaves or other parts of the papaya plant (Carica papaya) or fractionated components thereof are effective in the prevention or treatment of stomach cancer, lung cancer, pancreatic cancer, colon cancer or other solid cancers, or lymphoma, leukemia or other blood cancers, and are very safe with few side effects, making their medical significance extremely high. In addition, food compositions comprising a papaya plant (Carica papaya) extract component as an effe
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Method of Treating Malignant Mesothelioma

The present invention relates to a therapeutic agent for malignant mesothelioma comprising a substance which inhibits binding of CD26 to extracellular matrix such as an siRNA targeting CD26 cDNA or an anti-CD26 antibody. The present invention also relates to a method of treating malignant mesothelioma, which comprises administering the substance to a patient in need thereof. The present invention relates to a therapeutic agent for malignant mesothelioma and a method of treating malignant meso
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Endoplasmic Reticulum Targeting Liposomes

Provided are compositions that include lipid particles, such as liposomes, that can fuse with the ER membrane of a cell. The lipid particles can also deliver a cargo, such as a therapeutic or an imaging agent, encapsulated inside the particles inside the ER lumen of the cell. The compositions can be useful for treating and/or preventing diseases or conditions caused by or associated with a virus, such as viral infections, including HIV and HCV infections. The present application claims priori
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Material for Preventing Tissue Adhesion and Material for Preventing Joint Contracture

The present invention provides a tissue adhesion prevention material preparable at an affected area at the time of surgical procedure by producing a three-dimensional polymeric structure having a flexible structure and high solute permeability in a medium comprising water as the main component under mild conditions appropriate for body tissue components (i.e., at ordinary temperature and pressure) without conducting a chemical reaction or employing a physical procedure such as heating or light o
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Ultrasonic Probe Supporting Device

PROBLEM TO BE SOLVED: To move an ultrasonic probe along a kneecap to obtain ultrasonic echo information for the cartilage of a knee joint. ; SOLUTION: An attaching unit 16 is positioned by an attaching unit frame 58 to abut to the thigh with the knee in the bending state and a knee abutting part 62 to abut to the knee. The attaching unit has a probe support frame 60 for moving the ultrasonic probe along the kneecap. The probe support frame is rotated around the mechanical center of the knee join
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Knee Acoustic Matching Box for Ultrasonic Probe

PROBLEM TO BE SOLVED: To achieve acoustic matching between the kneecap and an ultrasonic probe when the ultrasonic probe is moved along the kneecap for obtaining ultrasonic echo information for the cartilage of a knee joint. ; SOLUTION: The acoustic matching box includes a water bag 64 filled with water and a water bag support frame 66 curved along the kneecap to hold and support the water bag from the left and right sides of the knee. The water bag is formed in a curved plate-like shape along t
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Imaging Technique

The present invention relates to a method of characterising tissue function in a subject in need of such characterisation. The method comprises performing an imaging technique, on a voxel defined within a tissue space of interest, wherein image data is generated over a time period during which the subject inhales gases with at least two different partial pressures of a paramagnetic gas. A compartmental model algorithm is applied to the image data generated for the voxel to provide information on
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Tissue Repair Scaffold

A tissue repair scaffold comprising a secondary fibre bundle, the secondary fibre bundle comprising a plurality of primary fibre bundles, each primary fibre bundle comprising a plurality of fibres, wherein the fibres comprise a biocompatible polymer. In embodiments the biocompatible polymer is polycaprolactone (PCL) (also known as poly-[epsilon]-caprolactone) and the average diameter of the fibres is less than 1 [mu]m. The scaffold is particularly adapted for tendon repair. Conventional appro
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Treatments and Prevention of Hydrocephalus

The present invention relates to the prevention or treatment of hydrocephalus. In one embodiment, the invention includes the use of one or more bioavailable folate derivatives, or salts thereof, for the prevention or treatment of hydrocephalus. Examples of more bioavailable folate derivatives that may be used include any combination of: folinic acid, tetrahydrofolate, thymidine, 10-formyltetrahydrofolate or methyltetrahydrofolate, or salts thereof. The invention also relates to a composition com
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Laser Imaging System to Assess the Vitality of Pulpal Chamber of Teeth

Brief Description Researchers have developed a laser imaging system that accurately assesses the pulp vitality of a tooth. This system can assess and image the pulp vitality without pain to the patient and the method used by the system is non-invasive. Advantages Pulp vitality test is crucial in monitoring the state of health of dental pulp especially after traumatic injury. Traditionally, thermal and electrical pulp testing methods have yielded false positive or false negative readings on
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Portable, Foldable Mirror

The present invention relates generally to mirrors and, more specifically, to a portable mirror having a plurality of foldable mirror panels, each hingedly attached to a main mirror panel. A light fixture is provided and sits at the top of the device, flush with the folding panels when in the closed position. A sensor is provided between the main panel and the foldable mirror panels and is activated when the panels are moved to an open position. The device provides easy viewing of feet for peopl
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Digital Staining for Histopathology

Background 99.9 % of cancer cases are diagnosed and monitored by histopathological assessment of a biopsy sample. The presence, concentration and distribution of biological molecules (such as nucleic acid, protein or lipids for example) can be determined by selecting a specific combination of chemical stains and fixatives. A commonly used stain in histopathology is hematoxylin and eosin (H&E) which detect nuclei and cytoplasmic components of tissue, respectively (Figure 1). A well-trained hist
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Mouse Models of Rhinovirus-induced disease

Background Rhinoviruses cause the common cold, hospitalization of infants, pneumonia in the immunosuppressed and the majority of acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD). These diseases generate hundreds of billions of dollars in healthcare costs, and the morbidity and mortality attributable to rhinovirus infections is enormous. No effective treatment or vaccine is available. Attempts to develop small-animal models of rhinovirus infection in many specie
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Bioresponsive Nanoparticle Aggregates

Background Bioresponsive materials offer a promising commercial opportunity and demonstrate great potential in the medical diagnostics and drug delivery sector. Compared with conventional chemistry, they are more sensitive, simple to use and more compatible with biological samples. Enzymes in patient samples such as blood and urine can be diagnostic of a number of disease conditions including HIV, Hepatitis C, various cancers, as well as Candida and MRSA infections. Hence bioresponsive materia
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Finger Fracture Fixation

Fractures of the finger bones are a common pathological issue which could lead to temporary or permanent loss of normal function. Fractures close to the joint lead to further complications, often resulting in permanent loss of the affected finger. The kinds of fractures encountered are i) incomplete: bone is not fully broken; ii) complete: clean fracture into 2 pieces; iii) multiple: bone is broken into more than two pieces; iv) comminuted: bone is shattered into several bits; v) transverse, obl
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Adjustable Knee Ligament Fixator

The need for a technique that allows post-fixation graft re-tensioning after anterior cruciate ligament (ACL) reconstruction is indicated by clinical observations of excessive anterior laxity in the immediate and early postoperative period in some people who undergo knee surgery. This may occur in cases where grafts are secured in both the femur and tibia by interference screws. The causes may include viscoelastic graft stretching and fixation failure. Soft tissue grafts may also slip past their
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Treatment of Mast Cell Related Disorders

Isis Project No 3566 CRAC channel blockers provide a novel therapeutic strategy for treating allergic rhinitis and nasal polyposis. Marketing Opportunity Allergy is characterised by an inflammatory response to seemingly harmless substances and it is likely that one in three people will suffer from the condition during their lifetime. Mast cells are pivotal to this inflammatory response. Indeed, aberrant mast cell activation is linked to a variety of allergic diseases including nasal polypos
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Efficient Cell Cryopreservation Media

Isis Project No 4026 Oxford researchers have developed cell cryopreservation media with well-defined, serum free agents for preserving adult stem cells, human and animal primary cells. Marketing Opportunity The market size of stem cell research products is estimated to be $800M and expanding through double-digit growth each year (Stem cell research products, by Bharat Book Bureau, 2008). The market of cell therapy including stem cell therapy is even greater. Oxford researchers have develope
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Prostate Cancer Treatment

New prostate cancer treatment that targets prostate cancer cells that have an elevated level of homologous recombination (a DNA repair pathway). Marketing Opportunity Most of the currently available treatments have significant side effects associated with them so quality of life is a major consideration when making treatment decisions. There is a real need to find more targeted approaches to treatment with fewer side effects. Prostate cancer is the most commonly occurring cancer in men i
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Amidobetaine solution for oral care

The patent includes an aqueous composition for oral care use comprising an amidobetaine solution. The solution includes the following five advantages: 1) The process of the present invention gives an amidobetaine composition that is most suitable for oral care applications (mouth rinses and tooth pastes) due to its significantly less bitter taste compared to commercially available CAPB for personal care applications. 2) The amidobetaine of the present invention allows use of a number of
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A Novel Therapeutic Strategy for HIV Infection

BACKGROUND: Tat is an HIV regulatory protein that is essential for viral replication and HIV disease progression. It critically regulates HIV transcription and allows the generation of full-length transcripts necessary for the formation of virus particles. Tat is also found in the circulation and can enter uninfected cells in an active form. Thus, Tat plays a role in the immune dysregulation and death of infected and uninfected T cells, helping to explain why the number of T cells that die each
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Human Osteoblastic Cell Line with Stably Knocked-Down Vitamin D Receptor

Vitamin D3 is implicated in a myriad of biological responses, many of them mediated via the vitamin D receptor (“VDR”). Researchers at the University of California have developed a human osteoblastic cell line in which VDR activity is substantially and stably reduced. The new VDR knockdown cell line provides an important tool for researchers studying vitamin D-mediated signaling and bone formation This cell line also allows for the rapid screening of synthetic vitamin D analogs to disting
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Reversible Inhibitors of Cytosine-C5 DNA Methyltransferase

BACKGROUND: In eukaryotes, the methylation state of a gene specifically affects its transcription. Proper function of the methylation enzyme is crucial for viable development and normal cellular activity. Increased activity of the enzyme cytosine-C5 DNA methyltransferase (DCMTase) has been directly associated with neoplastic development and excess methylation by DCMTase may constitute a key step in carcinogenesis. Currently, the drugs available for inhibiting DCMTase bind irreversibly to the enz
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Rapid Antimicrobial Susceptibility Assay

BACKGROUND: The rising incidence of infectious bacterial strains that are resistant to multiple broad-spectrum antibiotics poses serious complications to the treatment of infectious diseases. Absent knowledge of a given infectious agent's susceptibility to various antibiotics, physicians face the dilemma of prescribing an antibiotic that may prove to be ineffective, or prescribing an antibiotic capable of treating the most resistant strains but, if used unnecessarily, may accelerate the breeding
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A device for radiation therapy of rectal cancer

Colorectal cancer patients primarily undergo surgery for treatment followed by chemotherapy. The surgical procedure leads to development of a scar in the region of the body lower to the pelvic diaphragm and between the legs, known as perineal scar. During the radiation treatment, this perineal scar resulting from removal of rectum and anus, must also be included. The present invention relates to a novel device for pushing the scar towards the anterior section and properly positioning it during r
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Highly Hypoxia Specific Gene Expression System By the Combination of the Erythropoietin Enhancer and the Erythropoietin 3'-Untranslated Region for Ischemic Disease Gene Therapy

Gene therapy with VEGF (vascular endothelial growth factor) is a new potential treatment for ischemic heart disease. Currently, VEGF is the most effective therapeutic gene for neo-vascularization and there is a need for it to be regulated. This invention relates to gene therapy and to the compositions and methods for treating ischemic diseases and other diseases by localized hypoxic conditions. A highly hypoxia specific gene expression system has been developed by the combination of the erythrop
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An Integrated Wireless Neural Interface for Chronic Recording and Stimulation

The envisioned system integrates, wirelessly, a neural electrode array with a signal processor and a RF-communication module. The neural array can talk simultaneously with many individual neurons on the brain and the entire system can be implanted chronically inside the body thus eliminating the need for supplemental wiring and intermediate electrical and mechanical interfaces. <i>Benefits</i> Existing neural interfaces are implanted in the tissues with wires that come out and across the
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Cone-Beam Reconstruction Using the Backprojection of Locally Filtered Projections

Technology for X-ray detection in cone-beam geometry is rapidly improving and offers more and more potential for the construction of robust computed tomography systems for high-resolution volume imaging. In order to optimally build such systems, problem of cone-beam image reconstruction needs to be understood. Our technology consists of a new methodology for reconstructing images from cone-beam projection data. This methodology facilitates exact reconstruction with limited measurements and do
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Polymeric Compositions and Methods of Making and Using Thereof

Hydrogels are polymeric compositions, i.e. three-dimensional polymer networks that are capable of absorbing large amounts of water, and swell in water or aqueous fluids without dissolving. The wide variety of medical applications for polymeric compositions demonstrates the need for the development of different types of compositions with varying physical properties for use in various medical applications. It would also be desirable in some instances to have polymeric compositions that can be prep
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Drug Delivery System That Targets Bone and Boosts Availability and Solubility of Therapeutic Drugs

In healthy individuals, bone resorption and formation are well balanced, with the bone mass maintained in a steady state. Disturbance of such balance may lead to a number of bone diseases such as osteoporosis, Paget�s disease, osteoporosis and bone cancer. With the worldwide prevalence of osteoporosis alone estimated at approximately 96 million people in 2004, there is increasing need for effective therapeutics to delay bone disease progression, reverse disease effects, and improve the quali
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Novel Allele-Specific Primer Design for Quantitative, Sensitive, Allele Frequency Determination

The present invention provides a novel primer design for real-time quantitative allele-specific PCR (AS-PCR), which results in improved sensitivity, specificity and reproducibility of detection. The utility of this approach has been demonstrated by using quantization of JAK2 G88526T SNP somatic mutation under more than one kinetic PCR platform. <i>Benefits</i> The use of this primer design in quantitative kinetic PCR technique has very broad applications. Several examples are: � Detectio
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Metal-based Membranes with Reduced Autofluorescence to Improve Detection and Imaging of Surface Analytes

Increasing the concentration of cells and microorganisms by filtration for solid phase imaging and counting is a rapid, sensitive tool. In addition, filtration is used for purification of genomic DNA from biological samples, which is a requisite initial step in the conducting many molecular diagnostic assays. Fluorescent analysis of these small particles directly on the filtration membrane without a transfer step would increase efficiency and reduce cost. However, this is typically limited by m
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Process and Formulation to Improve Viability of Stored Human Cells and Tissue

There are more than 750,000 tissue and body-part transplants conducted annually, according to the American Association of Tissue Banks. There are many applications that utilize skin grafting: reconstruction after surgery; tissue replacement in burn victims and patients with epidermolysis bullosa (EB); treatment of chronic ulcers; and hair restoration to areas of alopecia. Fresh skin can only be stored for 7 days before cryopreservation is required. As such, most skin transplanted in the U.S. is
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Drug Delivery System for Macromolecular Treatment of Arthritis and Other Inflammatory Diseases

The technology in this invention is a water-soluble polymeric drug delivery system for the improved treatment of rheumatoid arthritis and other inflammatory diseases. The delivery system consists of a water-soluble polymer backbone, targeting moieties, and antirheumatic drugs. The polymeric background and the targeting moieties would direct the delivery system specifically to the disease lesions. The uniquely designed linkages that connect the drug to the polymer backbone will be cleaved spec
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A Family of Intracellular Cholesterol Transport Proteins: Potential Drug Targets for Modulation of Cholesterol Homeostasis and Treatment of Coronary Heart Disease and Assay for Activity

This invention provides for a novel therapeutic target for diseases characterized by defects in cellular cholesterol homeostasis. The inventors have identified a member of a family of proteins that catalyzes the incorporation of cholesterol into cellular membranes. This protein represents a novel target for the development of drugs to treat coronary heart disease and other defects of cholesterol homeostasis. An assay for the protein is also included which could be used for diagnostic and drug di
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Strategies for Engineering Precise Folding of Proteins, Peptides, and Antibodies

Peptide cyclization is a common strategy to engineer better polypeptide-based therapeutics, or other industrial proteins/peptides. Cyclization and crosslinking are achieved in nature using disulfide bridges. This invention consists of methods to create noncovalent crosslinks in peptides in a manner which simplifies chemical synthesis and guides polypeptide folding while simultaneously creating labeled compounds that can be traced in live animals using imaging techniques. <i>Benefits</i> Cy
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Alpha Conotoxin peptides and derivatives as potential therapeutics for nerve hypersensitivity and neuropathic pain

This invention includes alpha-conotoxin peptides isolated from the venom of predatory snails and related synthetic derivatives which antagonize the a9a10 nicotinic acetylcholine receptor, which has been associated with neuropathic pain. These peptides are more specific for this receptor subtype than currently available antagonists and represent a previously unrecognized mechanism for treatment of nerve hypersensitivity and neuropathic pain, including sciatica and diabetic neuropathic pain . .
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Method for Oral Decorporation of Metals

Several new diseases involving heavy metal poisoning and radionuclide exposure have been introduced to mankind due to the emergence of new technologies. In addition, the potential for exposure is expected to increase with the continuing industrialization of society. The administration of chelation agents represents the only therapeutic approach, other than symptomatic treatment, for the amelioration of metal and nuclide toxicity. Ethylenetriamine pentaacetic acid (EDTA) and diethylenetriamine
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�O Conotoxins, including MrVIB, with potent and long-lasting analgesic effects

This invention relates to peptides derived from cone snail venom, including MrVIA and MrVIB. These peptides block specific sodium channel subtypes which mediate pain signals in dorsal root ganglion neurons, indicating they may be useful treatment for neuropathic and inflammatory pain. <i>Benefits</i> ? Chronic neuropathic pain is estimated to affect up to 8% of the world�s population. ? Currently, no highly effective treatment is available. In 2005, the European and US neuropathic pain
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Proton-Conductive Nanoporous Opal Membranes

Well studied perfluorosulfonated ionomers such as Nafion� show decreased proton conductivity and degradation above 100oC, methanol permeation and swelling in methanol and water, which limits fuel cell operation to the narrow temperature range. This technology features an inorganic-organic composite membrane which possesses proton conductivity as a result of nanopore surface modification with organic molecules carrying acid functionalities. Self-assembled opal membranes allow a simple and power
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Raman Spectroscopy Apparatus and Method for Continuous Chemical Analysis of Fluid Streams

The present invention provides apparatus and methods for analyzing the chemical composition of fluid streams using Raman spectroscopy. The apparatus includes a laser source for producing light having an excitation wavelength. The light is introduced into a bundle of optical fibers connected to a tubular Raman enhancement cell. A transparent optical element (lens and/or window) acts as a barrier element to isolate the flowing sample stream from the optical components. The Raman enhancement cell i
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Steroid Profiling in Ovarian Follicular Fluid, Importance for Diagnosis, Prognosis, and Determining Strategies for Treatment

Very sensitive assays for assaying concentration and profile of steroid biomarkers in small volumes of ovarian follicular fluid have allowed identification of specific steroid profiles which are useful for diagnosis of specific endocrine disorders, and assessing ovarian function. Steroid profiles can allow definitive diagnosis of a variety of endocrine disorders affecting ovarian function and fertility, and will be useful for determining which modes of treatment might be effective. For e
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Steroid-Targeted Therapeutics for the Treatment of Cancer

Cancer is the second leading cause of death by disease in the U.S. It is essential to develop anticancer agents that demonstrate good efficacy without causing systemic toxicity. The cytotoxicity of anticancer agents can be increased by targeting them specifically to the tumor cells. It can be further augmented by directing the anticancer agent to specific subcellular compartments where the drug exerts its action. Dr. Kopecek and colleagues have created a system where drugs are first passively
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Targeted Nanoparticles Conjugated to Chelators as Imaging Agents and for Improved Targeting in Chelation Therapy

Excessive iron stored in the body is a powerful catalyst for the generation of highly toxic free radicals that can damage all molecular classes found in vivo. The progression of many diseases such as atherosclerosis, cancer, diabetes, and neurogeneration disorders can be arrested by depleting the excessive iron. The invention uses nanoparticle-conjugated chelators that are delivered to diseased organs to sequester or deplete iron. The conjugated nanoparticles can be biodegradable or non-biodegra
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Molecule Enhanced Optical Detection by Laser Action

There are numerous florescent molecules used as tags in both medical and biological research. These tags are very specific to the other molecules or sites to which they attach. When excited, the tags emit photons, which are then detected. This technology uses stimulated emission to increase the efficiency of detection by requiring a smaller number of molecules to have a detectable signal. <i>Benefits</i> Generally, the photon emissions from tags are spatially broad, making the collection of
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Medicinal Drugs and Health Products from Natural Sources for Treating a Wide Range of Diseases

Areas of rich biodiversity, such as among the tropical forests and coral reefs of the South Pacific, are home to a wide pharmacopoeia of natural molecules which have evolved to regulate the interactions of an organism with its neighbors. Drs. Chris Ireland and Louis Barrows of the University of Utah are leading an international collaborative research effort to assess these biological interactions in order to identify novel medicinal compounds. In conjunction with numerous investigators at an a
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Ligation-During-Amplification (LDA) for DNA modification

Polymerase chain reaction has traditionally been used for in vitro modification and amplification of linear DNA. On the other hand, circular closed loop DNA is often the form replicated in host cells. In order to modify host cell DNA constructs, the traditional method has been to work with linearized DNA that must then be cloned back into a circular form before its reintroduction into the host cell. Ligation-during-amplification (LDA) represents a novel approach for the in vitro amplification
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GST polymorphic allele assay

Cancer is the second largest cause of death in developed countries, accounting for nearly one in five of all deaths. Polymorphisms of glutathione S-transferase (GST) enzymes have been correlated with altered risk of several cancers, as well as altered response and toxicity from cancer chemotherapy. GST�s are a family of detoxifying enzymes which inactivate both environmental pollutants and many medications, such as chemotherapeutic agents. Dr. Keller and colleagues have developed a low cost
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Vaccine Candidate Genes Directed Against Virulent Group B Streptococcus

Group B Streptococcus algalactiae (GBS) is the most common cause of bacterial infection in human neonates. Type III GBS is responsible for most bacterial meningitis and a large proportion of other invasive infections. Over 90% of type III infections are caused by a genetically related group of organisms (type III-3). Using subtractive techniques, the inventors have pulled out two genes, Spb1 and Spb2, unique to type III-3 GBS which code for probable for surface proteins. Adhesion to and invasion
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Siah-1 as a Drug Target for Colorectal Cancer Chemotherapeutics

Colorectal cancer is the second-leading cause of cancer death in the U.S. The American Cancer Society estimates that 56,730 people will die from colorectal cancer in 2004. Sporadic colon polyps and carcinomas carry mutations in the Wnt signaling pathway, which is responsible for degradation of �-catenin. Increased �-catenin promotes cell proliferation and transformation and inhibition of apoptosis; making the Wnt pathway a good target for possible chemotherapeutics. The inventors have sh
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Specific Covalent Immobilization of Proteins through Dityrosine Crosslinks: New Chemistry to Improve Protein Arrays

This invention provides a new immobilization chemistry that combines the versatility of specific protein immobilization with the advantages of site-specific covalent crosslinking. Target proteins are first reversibly surface associated through the well-known interaction of His6-peptide tags and NTA-Ni(II) complexes. Upon addition of an oxidant, a co-localized peptide-Ni(II) complex catalyzes covalent crosslinking between tyrosine residues strategically placed adjacent to the His6 peptide tag an
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Supported Opal Membranes

Membranes with nanopores are important in molecular transport, molecule-surface interactions at the nanoscale, and in separations. This invention relates to suspended opal membranes formed by the self-assembly of sub-micron sized silica spheres. The colloidal solutions of 440 nm or 170 nm diameter silica spheres is evaporated over vertically oriented 0.3 mm silicon wafers with square openings. Robust 5 � 5 mm membranes, with regular (111) packing and three-dimensional pores (75 nm or 40 nm in
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Homogenous bioassays and biosensors.

This invention discloses a novel system that involves the use of a short peptide antigen doubly-labeled with fluorescent dye for use in homogenous bioassays and biosensors. Fluorescent energy transfer dyes, which are capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reag
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Compositions and Kits for Fluorescence Polarization Assay of Large Molecules

This invention describes a methodology for determination of high molecular weight antigens based on the principles of fluorescence polarization immunoassay (FPI). An improved oligopeptide composition for use in a fluorescent polarization immunoassay for a high molecular weight analyte is disclosed, along with a kit and a method using the composition. The composition comprises an oligopeptide selected by a screening procedure in which a plurality of different oligopeptides having respective amin
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Bioresponsive Polymers Effective in Contraception, Hormone Delivery, and Protection against STDs

Women across the globe face a serious health threat from preventable STD and HIV infections due to the lack of compliance from partners in condom use. Development of a safe and effective method for prevention has a broad market. Currently available methodologies, such as the female condom, sponge etc., are only marginally effective. This technology would provide a better and more effective alternative for women. This is a biopolymer that would be applied to the vagina or anus precoitus and u
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Novel Approaches for the Treatment of Nephrogenic Diabetes Insipidus (NDI)

This invention discloses a novel set of interactions between the prostaglandin and purine signaling pathways that reveal a new class of drug targets for future NDI therapies. <i>Benefits</i> Nephrogenic Diabetes Insipidus (NDI) can be acquired as a result of the use of medicines such as lithium for bipolar disorders, or others such as cisplatin, puromycin, adriamycin, calcium channel blockers, and amphotericin B. Conditions such as chronic kidney disease, electrolyte disorders (high calci
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Method of High-Throughput Drug Screening

The development of effective drugs remains a challenging, risky and expensive endeavor. Currently it takes an estimated 7-10 years to develop and market a drug at a cost that exceeds $800 million. In addition, advances in combinational chemistry are resulting in a large supply of chemical compounds that need to be screened for their efficacy in fighting disease. However, high-throughput drug screening (HTS) is emerging in the field as pharmaceuticals and biotechnology companies compete to becom
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Noninvasive Thermal Triggering of Hybrid Hydrogels

It is now well recognized that when systemic chemotherapy is used in the treatment of solid tumors, it is almost impossible to achieve therapeutic levels of drug at the tumor site without damaging healthy organs and tissues. One solution to this problem is to encapsulate the drug in a biocompatible material that can be injected into the blood stream with the intention of delivering drug to a diseased site, (e.g., solid tumor tissue). The single biggest challenge now facing drug delivery (for lip
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Hydroxy Alkyl Celluloses that Melt at Body Temperature for Food Preparation and Drug Delivery Applications

Cellulose derivatives, owing to their excellent properties and physiological safety, are used widely as thickeners, adhesives, binders and dispersants, water-retention agents, protective colloids, stabilizers and suspension, emulsifying and film-forming agents. Dr. Kiser and his colleagues have discovered that a series of biocompatible GRAS (generally regarded as safe) anions allow certain cellulose derivatives to melt and go from a gel below body temperature to a liquid at body temperature. T
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Novel Genetic Mutations Affecting Intelligence

Decreased mental acuity can stem from multiple sources; i.e., from head trauma, age related diseases, and inherited genetic defects. An estimated 4.5 million Americans have Alzheimer�s disease or some other form of dementia requiring extensive medical and family care. Mental retardation affects approximately 1 to 3% of the general population and has a large social impact from increased incarceration rates, increased unemployment and increased reliance on welfare. Certain forms of mental retar
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Inhibitors of Ubiquitin Isopeptidases as Pharmaceuticals

For a cell, protein degradation is as essential as protein synthesis. One important intracellular mechanism for protein breakdown is the ubiquitin-proteasome pathway, which functions widely in intracellular protein turnover. In preventing cancer, it is important that inactive tumor suppressor proteins be destroyed to allow for the normally active proteins to elicit their function of inhibiting tumor growth. Defects in this pathway can lead to deregulation of these controls, and thus inhibitor
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Improved Antithrombin III for Circulatory and Cardiovascular Diseases

Blood vessels are lined with heparin-like molecules that serve an anticoagulant function and help to keep blood flowing through the circulatory system. Many artificial vessels and medical devices also feature heparin-coated surfaces that prevent blood clot formation and sustain flow. Heparins require the binding of antithrombin III (ATIII), an endogenous plasma protein, to express their anticoagulant properties. Under inflammatory conditions, endogenous ATIII can break down, allowing clotting
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Circular, Hairpin, Circular/Hairpin, Lariat, and Hairpin-Lariat Hammerhead Ribozymes

Ribozymes are RNA molecules that can catalytically cleave nucleic acids, thereby suppressing expression of unwanted gene products such as disease causing proteins. This technology includes various configurations of the hammerhead-class of ribozymes that contain circular, hairpin, circular-hairpin, lariat, and hairpin-lariat ribozyme domain structures. They are catalytically active and provide advantages not present in the wild-type hammerhead configurations. Their production can be accomplish
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Erythropoietin as a Tissue Regenerating Cytokine for Acute Renal Failure and Other Kidney Diseases

Acute Renal Failure (ARF), in the context of multi-organ failure and in high risk patients, remains a common, and often fatal (~50% mortality), complication. Preventative measures are only poorly effective, and dialysis therapy is expensive and time consuming. ARF is characterized by cell injury and death by necrosis and apoptosis in the tubular epithelium. Consequently, recovery from ARF depends on the reestablishment of cells in the damaged area via processes that require surviving cells to
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Assay and Drug Target for Ewing's Sarcoma

Ewing�s sarcoma is a highly aggressive bone or soft tissue-associated tumor of children and young adults. The tumor frequently progresses undetected until it metastasizes, whereupon the mortality of the disease greatly increases. This technology reveals a new molecular marker, NKX2.2, which has high specificity and sensitivity for Ewing�s sarcoma. In addition to being a diagnostic marker, NKX2.2 plays an active role in the progression of Ewing�s sarcoma and its pharmaceutical targeting
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Cancer Systems Biology Website

As biological knowledge continues to grow, searching for how physiological systems function and how they relate to a particular disease becomes ever more difficult. In order to improve the efficiency whereby potential research targets might be identified, this work features an internet portal providing access to information for directing cancer research in a methodical, systems-based approach. The database portal includes interactive tutorials and guides to link physiological systems as well a
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Hydrogels Formulated for in situ Gelation Effective for Drug Delivery and Wound Healing

The high water content and soft consistency of hydrogels make them similar to natural living tissue with numerous applications across the medical field. Under certain conditions it is desirable to prepare a hydrogel at the site of use; unfortunately most polymers must be preformed due to the extreme reaction conditions, which would not be compatible with the environment of use. Kiser and Roberts have overcome this problem with the creation of in situ gelling hydrogels. Two liquid-state prepolyme
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In Vivo Use of GST-activated Nitric Oxide Donors to Treat Cancer and the Multidrug Resistance Phenotype

A class of enzymes known as Glutathione-S-Transferases (GSTs) is over expressed in many forms of leukemia and solid tumors. This over expression is a major mechanism whereby malignant cells acquire drug resistance. Nitric oxide (NO) is a major biologic effector that inhibits tumor cell growth. A major problem with widespread in vivo use of NO is its non-specific action on non-cancerous cells including its effects on vascular tissue resulting in hypertension. This invention defines a number o
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Biodegradable Elastomers for Medical Use

Polyurethanes are widely used in the medical field due to their wide range of physical properties and biocompatibility. However, they do not have the inherent ability to biodegrade. This invention addresses this by placing linkages of biodegradable esters between poly (urethane) blocks to create a biodegradable poly (ester-urethane). Biodegradation of the elastomer upon exposure to physiological conditions results in products biocompatible with a patient, such as lactic acid, glycolic acid, a
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Mycoplasma Arthritidis Mitogen (MAM) Superantigen: a Unique Immunomodulator

MAM is a superantigen (SAg) produced by M. arthritidis, a bacterial species that causes murine arthritis, toxic shock and dermal necrosis. MAM has very little sequence homology with any other SAg, but it is a potent immunomodulator of murine and human T-cells, B-cells, NK cells and macrophages. In this invention, the MAM molecule was expressed in E. coli, and the inventors found that MAM is not notably toxic when injected into mice, but a single minute does profoundly influences the immune syste
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Selective Inhibitors/Activators of PDE3 for Treatment of Heart Failure and Vascular Disease

Nonselective cyclic nucleotide phosphodiesterase (PDE3) hydrolyzes cAMP and regulate its content in cardiac myocytes. PDE3 inhibitors are already used clinically in the treatment of heart failure and peripheral vascular disease. The increased morbidity and mortality seen with the long-term administration of these agents in patients with heart failure severely limits the benefits that can be achieved through their use. Dr. Movsesian has identified new PDE3 isoforms in human myocardium that are
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Feedback Dose Control in Thermal Therapy

The American Cancer Society predicted that 1.372 million Americans would be diagnosed with cancer in 2005 and 570,280 would die of it. Improved treatment is imperative for reducing this staggering death rate. Hyperthermia is a promising noninvasive cancer treatment modality that involves the use of elevated temperatures. It can be used for tissue ablation alone or used in conjunction with radiation and chemotherapies. Studies have shown a significant reduction in tumor size when hyperthermia
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Quercetin: a Natural Supplement for Treating and Preventing High Blood Pressure (Hypertension)

This invention defines a nutritional supplement formulation containing an effective amount of quercetin that is specifically dedicated to ameliorating, delaying and/or treating hypertension. An effective dose of quercetin may be administered over the course of multiple time periods or once per day. <i>Benefits</i> High blood pressure (hypertension) is known to cause cardiac hypertrophy; i.e., abnormal growth and remodeling of the heart. The stimulus for abnormal cardiac growth is an incr
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Methods and Apparatus for Minimally Invasive Transverse Aortic Banding: a Useful Tool in the Study of Pathophysiology and Treatment of Pressure-Overload Cardiac Hypertrophy

The present invention provides a curved suture applicator to enable a surgeon to efficiently encircle a target tissue such as a blood vessel with a suture or thread, and a minimally invasive ligation method that utilizes the curved suture applicator to efficiently band the transverse aorta of small mammals for producing left ventricular (LV) pressure overload. With the use of the invented methods and apparatus, one can quickly and reproducibly band the transverse aorta with low surgical morbidit
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A New Target for Allergy and Asthma Treatment

Eosinophils are a tissue dwelling hematopoietic cell type that likely play a role in parasitic immunity and allergic disease, such as asthma. Activated eosinophils secrete toxic basic proteins such as Major Basic Protein (MBP) which are postulated to cause bronchial hyper-reactivity, damage of the bronchial mucosa, and remodeling of the airways. Our inventorsat the University of Utah have identified a nested RNA gene that regulates human eosinophil development. They have been able to design a
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Rational Design of Anxiolytic and Anticonvulsant Drugs

The balance between neuronal excitation and inhibition is critical for information processing in the brain. When this balance is disrupted, epilepsy and anxiety disorders may result. This balance depends on inhibitory neurotransmission mediated primarily through GABAA receptors. These receptors can be potently modified by endogenous neurosteroids. However, existing drugs have serious side effects because they are relatively indiscriminate, affecting many GABAA receptor subtypes. A subtype-s
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Central Medical Image Storage Respository

This invention outlines the establishment of a central digital medical image repository that can be used by multiple institutions within a region or multi-site radiological imaging network. The invention streamlines and reduces the cost of imaging storage and retrieval when compared to traditional transfer of images to silver halide film for interpretation. <i>Benefits</i> The development of PACS (picture archiving and communication systems) has begun to eliminate the need to produce conve
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Scanning Probe Microscopy Based Diffusion of Dopant and Impurities

It is common to see devices shrink in size with time, especially electronic devices such as computers, cell phones, mobile music players, pagers, calculators etc. There are, however, some important working components in these devices that cannot be replaced by an alternate component but have to be designed in such a way that they fit the shrunk size. As the device size is reduced, conventional lithography techniques used for making these devices become hard to implement (in some cases useless).
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Computerized Medical Record for Labor, Delivery, Postpartum, & Nursery

This invention, nicknamed OB.exe, comprises a computerized medical record and a database system that actively manages information on normal laboring women and term newborns. The database captures 800 data elements representing more than 90% of the patient�s clinical data (excluding fetal monitoring). These data elements are entered and viewed via graphical user interface (GUI) forms or screens. Most of the data entry is via picklists that facilitate rapid, organized information retrieval an
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Method for producing Beta-Carotene on a Large Scale for use in the Medical, Food and Cosmetics Industry

Carotenoids are antioxidant micronutrients reported to protect against the adverse activity of free redicals. Among the carotenoids, Beta-carotene is the most recognized and widely known carotenoid. Flavobacterium multivorum ATCC 55238 is best known for zeaxanthin production, but it was culturally manipulated under the influence of salts such as urea and sodium carbonate to produce a high amount of Beta-carotene. The amount of Beta-carotene accumulated was the highest ever reported from bacteria
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Image Guided Interstitial Ultrasound Therapy

The Technology The probe’s delivery system includes five independent channels, each capable of producing up to 50W of RF power. An MRI-compatible motor has also been developed to control the rotation of the heating applicator inside the bore of a clinical 1.5T MR scanner. The capability to perform real-time quantitative thermometry and control during heating with these applicators has been demonstrated with MRI. Initial in-vivo trials show very promising results. The Challenge Cli
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Mitochondria Penetrating Peptides

The Background Recent advances in the field of mitochondrial medicine have provided researchers with further incite into the unique structural and functional roles of the mitochondria. Converging lines of evidence have demonstrated the potential role of the mitochondria in a variety of diseases including cancer, cardiovascular disease, diabetes and neurodegenerative diseases. Consequently, new directives in the field are aiming to develop novel targeting strategies and therapeutics.
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Improved Enzymatic Synthesis of Oligosaccharides

Enzymatic synthesis of oligosaccharides, while a great improvement over chemical methods, remains expensive due to the difficulty in producing the nucleotide diphosphate sugars (NDPG’s: e.g. uridine diphosphoglucose, UDPG) that are the substrates for the glycosyl transferases. The Withers group at the University of British Columbia has developed methods of using simple, readily made, glycosyl fluorides, in the presence of the appropriate nucleotide diphosphate, as alternate substrates for NPDG
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Human Lipase Cell Lines

Human hepatic lipase cell line – Chinese hamster ovary (CHO) cells have been stably transfected with a human hepatic lipase cDNA to overexpress this enzyme in cell culture. CHO-HL-Lec1 – This cell line secretes human hepatic lipase in a form with uniform glycosylation to facilitate downstream crystallography studies and structural analysis of human hepatic lipase. This is achieved by using a mutant strain of Chinese hamster ovary cells which is associated with a mutation in carbohydrate proc
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ABCA1 Monoclonal Antibodies

ATP-binding cassette subfamily A member 1 (ABCA1) plays a pivotal role in the production high-density lipoprotein (HDL) and is a member of the ATPase cassette superfamily of transporters. ABCA1 mutations are associated with impaired cholesterol efflux and reduced plasma HDL which are characteristic features of Tangier disease and familial hypoalphalipoproteinemia. Conversely, the overexpression of ABCA1 in animal models has been shown to be protective against atherosclerosis. Researchers at The
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Retinitis Pigmentosa Animal Models

Retinitis pigmentosa is a human genetic disorder that causes blindness due to death of photoreceptors. Many cases of retinitis pigmentosa are caused by a mutation in the rhodopsin gene, rhodopsin P23H. This disorder has been modeled in transgenic Xenopus laevis by expressing the bovine rhodopsin cDNA containing the P23H mutation. An assay has been developed which allows quantification of photoreceptor death using an antibody that detects total rhodopsin. The expression of the bovine P23H rhodops
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Laser Technique for the Evaluation of Erosion Resistance of Insulating Materials

Dry band arcing is a phenomenon encountered due to the prolonged exposure of high voltage insulation to wet/polluted environment and it is the principal factor that is responsible for degrading the insulating materials by tracking and erosion. Predicting the lifetime of insulators is therefore important to engineers and also to the manufacturers of insulating materials. The conventional ASTM D2303 is an inclined plane test method usually used for evaluating the tracking and erosion resistance of
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In-torch Vaporization (itv) Sample Introduction System for Inductively Coupled Plasma Spectrometers

The University of Waterloo has developed a revolutionary sample introduction system for ICP spectrometers that provides dramatically more efficient and significantly increased analysis sensitivity of liquid samples using as little as nano-litre volumes (eg. a single cell). The system also enables the uniquely novel capability to analyse solid samples down to nano-gram weights (eg. one grain of pollen). ICP spectrometry is a well established routine technique used in the multi-billion dollar
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Apparatus and Method for Cell Culture

Typically, cells are cultured in vitro by growing cells in a fluid or gel-like growth media in a sterile container under controlled temperature, pH and perhaps dissolve oxygen. In many stem cell cultures, for example, cells are grown as free-floating aggregates in suspension in a media. However, in such cultures, the immediate vicinity of each cell, its microenvironment, is constantly changing such that a cell is exposed to a variable and uncontrolled local environment. This heterogeneity result
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Electric Field Treatment Chamber

The electro-mechanical instability of cell membrane to electrical field is a phenomenon that has been shown to inactivation of micro-organisms in liquid media. This has led to the development of a method referred to as cold-pasteurization for the treatment of liquid foods. The technique has many advantages over conventional heat treatment methods in that it preserves the natural quality of the foods. Since the method is based on the use of pulsed electric fields (PEF), it is energy efficient.
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Patient Initiated Emergency Response System (PIERS)

More than one million Americans will suffer an acute myocardial infarction (AMI) this year. Within the first 60 minutes of chest pain, 250,000 will die. Before reaching any medical attention, a total of 375,000, (one-third) will die. Current studies strongly suggest that time-to-treatment is an important predictor of mortality in AMI and efforts aimed at reducing the delay between AMI onset and therapy can reduce mortality significantly. Thus, strategies having even a small relative benefit in t
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Polygraph Sensor Simulation and Testing System

Polygraph testing invloves the measurement of four physiological responses: blood volume in the arim; respiration rate and volume in the thoracic and abdominal areas; and skin conductivity. Automated polygraph scoring algorithms have been developed to assist polygraph examiners to evaluation polygraph examinations. Each algorithm is designed for use with a specific signal interface usit because differences exist in the way that sensor signals are processed and sampled between different polygraph
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Computer-Controlled Neurological Stimulation System

Implanted electrical stimulation devices have been employed in the management of chronic intractable pain for over twenty years. The most common electrode implantation site has been the dorsal spinal epidural space. Spinal cord stimulation, like peripheral nerve or thalamic stimulation, evokes paresthesias at amplitudes that produce analgesia; the location of these paresthesias varies with electrode location. Empirically, it has proved to be necessary for therapeutic effect that these paresthesi
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A Microfabricated Array Sensor for the Electrochemical Detection of Biological Molecules

The ability to detect ultra low concentrations of heme and hemoglobin in bodily fluids has great value in clinical and medical diagnostic applications. Clinical screening for many diseases, such as colorectal cancer and malaria, is predominantly related to the concentrations of hemoglobin or heme in different biofluids. The detection of porphyrins in urine, saliva, cerebrospinal fluid and swabs from the nasopharyngeal cavity may also be used to indicate infection, trauma or other frank diseases.
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Cortisol Sensor for Soldier Stress and Fatigue Monitoring

The hormone cortisol, which is produced by the adrenal gland and is released in the body during stressed or agitated states, has gained widespread attention as the so-called "stress hormone." But this hormone is more than a simple marker of stress levels- it is necessary for the functioning of almost every part of the body. Excesses or deficiencies of this crucial hormone also lead to various physical symptoms and disease states. This compound has been studied as a biomarker for many diseases su
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Airborne Pathogen Neutralization

So far there is no effective way to disinfect or neutralize airborne pathogens in large volumes of contaminated air in real time to protect citizens against a terrorist attack using biological weapons, or to disinfect air in hospitals. The present inventions provide an apparatus and methods for neutralizing airborne pathogens in large volumes of ventilated air in real time, which is effective against airborne pathogenic bacteria, spores and viruses. The technology is based on the formation of
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Noninvasive Magnetic Sensing of Human Prostate Tumor

Despite the ability of noninvasive electromagnetic bioimpedance methods to measure low contrast changes in tissue, a perennial problem has been the precise localization of the change. Generally, the interrogation or sampling volume is gauged by the diameter of the coil used to impart the electrical signal to the tissue. Because of the intervening tissue between the coil and the region under study (e.g., skin, muscle, bone), it is difficult to precisely locate the depth of the physiological chang
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Enhancer of Luminol-mediated Chemiluminescence

The NADPH oxidase of phagocytic leukocytes generates superoxide anion, a toxic oxygen radical important to bacteriostatic and paracitocidal inflammatory responses. Ongoing studies of the oxidase, the components that comprise it, and the signaling pathways leading to its activation are of interest to basic and clinical research, and to clinical diagnoses. At present, the most sensitive method for measuring rates of superoxide production in leukocytes is luminol-mediated chemiluminescence.
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TH1 Vaccines Based on Replication Attenuated Avirulent Uracil Auxotrophs of an Obligate Intracellular Apicomplexan

Toxoplasma gondii is an obligate intracellular parasite capable of infecting most warm-blooded vertebrates and most nucleated cell types. Parasite transmission occurs primarily by oral ingestion of tissue cysts in undercooked meat or sporozoites in contaminated soil, food, or water. Natural infection typically results in an asymptomatic primary infection that leads to a chronic latent infection. Dartmouth researchers have now generated a replication attenuated avirulent uracil auxotroph
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CD161 Ligand, Pilar, for Modulating Activiation and Proliferation of T Cells

CD161 is a C-type lectin receptor initially associated with murine NK cells. Unlike murine lymphocytes, the expression of CD161 in human T cells identifies mostly memory lymphocytes and only a small proportion of invariant NK T cells. CD161 engagement in humans has been recently associated with the enhancement of IFN-γ and TNF-α production in the context of a T cell receptor (TCR) signal, while inducing the production of IL-12 by dendritic cells. The only previously identified ligand of CD161
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Compounds for Inhibiting, Arresting and Possibly Reversing Non-enzymatic Glycation in Skin

Non-enzymatic glycation and cross-linking (Maillard reaction) are well known processes which accelerate the aging of key biological molecules. For example, non-enzymatic glycation of macromolecules such as proteins and certain phospholipids (e.g., phosphatidylethanolamine and phosphatidylserine) appears to play an important role in the evolution of diabetic complications. Moreover, glycation is involved in aging of structural proteins such as collagen and elastin. The first two intermed
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Methods for Determining Collateral Artery Development in Coronary Artery Disease

Coronary artery disease (CAD) is the most common cause of morbidity and mortality in industrialized societies. Collateral arteries function as “natural bypasses” effectively restoring the blood flow to compromised tissues and play an important physiologic role in promoting survival and protecting tissues from ischemic damage. Circulating monocytes have been suggested to play a role in the collateral formation. Abnormalities in monocyte function then may be one of the factors responsible for
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Prognosis and Treatment of Breast Cancer

Spot 14, also known as thyroid hormone-responsive Spot 14 (THRSP) or S14, is a primarily nuclear protein that is abundant in tissues active in long chain fatty acid synthesis, including the lactating mammary gland. It has been shown that the S14 gene is located on chromosome 11q13 and is overexpressed in most breast cancers. Dartmouth researchers have now found that S14 is a marker for aggressive breast cancers. High-level S14 expression exhibits a strong positive correlation with tumor
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compositions and methods for inhibiting sonic hedgehog activity

Lung cancer is the most common cause of cancer in men and women in the United States. Lung cancer can be divided into two distinct classes, non-small cell lung carcinoma (NSCLC) and small cell lung carcinoma (SCLC). While constitutive Hedgehog signaling has been demonstrated in SCLC, this class represents only 20% of all lung cancers. Dartmouth researchers have now found that the Hedgehog pathway is constitutively active in a subset of NSCLC. Moreover, it has been found that overexpress
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methods for increasing cardiac MAss and performance

Congestive heart failure (CHF) is a chronic, degenerative condition that impairs the heart's ability to pump blood at normal filling pressures to adequately meet the energy requirements of the body. Multidrug treatment regimens that include diuretics, vasodilators and inotropic agents such as angiotensin-converting enzyme inhibitors, can slow the progression of CHF and reduce the number of acute episodes. However, treatment remains directed at symptoms and is most effective in the early stages o
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Method for Detecting Cardiac Collateral Formation

Coronary collateral circulation has been recognized as an alternative source of blood supply to an ischemic myocardial area. Collateral artery formation is associated with smaller infarcts, less ventricular aneurysm formation, improved ventricular function, fewer future cardiovascular events and improved survival. Dartmouth researchers have now found that there is a strong positive correlation between increases in the presence or formation of collateral arteries and N-terminal probrain n
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compositions and methods for preventing or treating variola virus infection

Variola virus, the most virulent member of the genus Orthopoxvirus, specifically infects humans and causes smallpox. Although smallpox was eradicated in 1980, it remains a potential agent for bioterrorism. As a category A biological weapon, its potential to devastate populations is a concern. Moreover, despite the efficacy of a live vaccinia virus vaccine, the level of toxicity is deemed unacceptably high due in part to the fact that a live vaccine can be lethal to immunosuppressed individuals s
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Transgenic Non-human Animal Model of Lung Tumorigenesis

Lung cancer is the leading cause of cancer mortality for men and women in United States. Key molecular changes, especially at early stages of carcinogenesis, represent potential pharmacological targets for lung cancer chemoprevention and therapy. Aberrant expression of cyclin E has been frequently observed in premalignant lung lesions, indicating that this is an early step in lung carcinogenesis. Overexpression of cyclin E also occurs in overt non-small cell lung cancers (NSCLCs) and has prognos
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methods for diagnosing and treating neuroendocrine cancer

Neuroendocrine cancers generally arise from the neuroendocrine system, a diffuse system in which the nervous system and the hormones of the endocrine glands interact. Neuroendocrine cancers also arise from non-endocrine cells which acquire some of the properties of neuroendocrine cells through an oncogenic process such as Selective Tumour gene Expression of Peptides essential for Survival (STEPS). Most of the well-described adult neuroendocrine tumors are distinctive and arise from a known prima
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Novel Inhibitor of Hedgehog Dependent Tumors

Sonic Hedgehog (Shh) plays a critical role in the development of numerous structures. Overexpression of Shh has also been implicated in a number of common human tumors, including lung and pancreatic cancer. Tumor cell lines appear to be dependent upon Shh for their survival with Shh acting as a mitogen for these cell lines, as recombinant Shh is sufficient to induce cell division. Dartmouth researchers in collaboration with the colleagues from Children’s Hospital Medical Center in Cin
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A Novel Class of Modulators of the Abc Transporter Family to Improve the Outcomes of Treatment for Cancer Therapy, Secretory Diarrhea, and Cystic Fibrosis

ATP-binding cassette (ABC) transmembrane proteins function to transport small molecules such as drugs across membranes. Dartmouth researchers have identified a protein, which is secreted by the bacterium Pseudomonas aeruginosa, which selectively reduces plasma membrane expression of ABC transmembrane proteins. Inhibition of the expression of these ABC transmembrane proteins in the plasma membrane by this protein or active fragments or mimetics thereof is expected to be useful in promoting delive
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Chimeric Nk Receptor and Methods for Treating Cancer

Natural killer cells are innate effector cells serving as a first line of defense against certain viral infections and tumors. Natural killer cells have the ability to recognize and kill a variety of different tumor types using specific cell surface receptors to recognize tumors. These activating NK cell receptors that recognize tumor cells have been identified in mice and humans natural killer cells. Dartmouth researchers have now found that a chimeric NK receptor molecule composed of
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Drug Resistance and Gene Expression in Biofilms

Biofilms are communities of microorganisms attached to a surface. Biofilm formation in clinical settings, particularly on a variety of medical implants (such as catheters) results in antibiotic resistant infections. These implant-based infections cause prolonged hospital stays and increase in health care costs approaching $1 billion dollars annually in the U.S. The mechanism of biofilm resistance developed by biofilm-grown bacteria differs from the resistance mechanisms utilized by free-swi
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Methods of Prevention and Treatment of Diseases Caused By the Fungal Pathogen Candida Albicans

The yeast Candida albicans is the most common fungal pathogen of humans. Most adult females experience sporadic incidents of vaginitis during the course of their reproductive lives and recurrent disease is not unusual. Oral lesions termed thrush regularly afflict infants and immunosuppressed adults with HIV or other forms of immunosuppression. Mucosal disease may serve as a conduit for the organism to invade the vasculature, enter the bloodstream, seed internal organs to cause extensive morbid
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Compositions and Methods for Inhibiting the Synthesis Or Expression of MMP-1 for Treating, Preventing, Or Managing

Overexpression of MMP-1 is associated with several pathological conditions, including the irreversible degradation of cartilage, tendon, and bone in arthritis and the degradation of collagens I and III in tumor invasion and metastasis. Patients with tumors that express MMP-1 have an overall poorer prognosis than patients with tumors that do not express this protein. Dartmouth researchers have now found that the expression of a MMP-1-specific short interfering RNA molecule in a breast can
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A Novel Pharmacological Pathway which Destabilizes Lysosomes and Targets Oncogenic Or Other Aberrant Proteins for Destruction

Lysosomes are acidic organelles that contain hydrolytic enzymes (such as proteases) that are required for maintaining normal cellular physiology. Lysosomal enzymes participate in diverse cellular functions from degradation of endogenous abnormal or normal proteins to activation or regulation of essential cellular processes. Interfering with lysosomal function can lead to cell death as found in the case of treatment of malarial parasites. The rise in pH of acidic food vacuoles (lysosomes in eu
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Compositions and Methods for Preventing and Treating Cancer Via Modulating UBE1l And/or ISG15

Individuals with acute promyelocytic leukemia (APL)(FAB M3) express oncogenic promyelocytic leukemia (PML)/retinoic acid receptora (RARa). All-trans-retinoic acid (RA) treatment causes complete remission of APL through induction of leukemic cell differentiation. A hallmark of the RA response in APL is PML/RARa degradation that reverses PML/RARa oncogenic effects. Proteasomal inhibitors prevent PML/RARa proteolysis, despite RA treatment, which is indicative of a proteasome-dependent pathway in th
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Composition and Methods for Enhancing and Detecting Prion Protein

Prion diseases such as Creutzfeldt Jakob Disease (CJD), bovine spongiform encephalopathy (BSE), chronic wasting disease (CWD), and scrapie are fatal, infectious, neurodegenerative diseases that occur in humans and other mammalian species. The conversion of a normal host prion protein (PrP) into a protease-resistant state (PrPres) is the most specific biochemical alteration in individuals affected by prion disease. A rapid and sensitive diagnostic test for early detection of prion disease is ur
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Novel Immunotherapy for Ovarian Cancer

Dartmouth investigators and their colleagues at the University of Kansas have discovered a novel method for rescuing cytotoxic T lymphocytes from destruction by ovarian carcinoma cells in vitro using an animal model system. Ovarian cancer causes more deaths than all other gynecologic cancers combined. Approximately 19,000 new cases of ovarian cancer are reported and 12,000 deaths occur annually. Ninety percent of ovarian cancers are ovarian carcinoma, i.e. epithelial cell-derived. Ova
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Use of Monocyte Chemoattractant Protein-1 (MCP-1) for Treatment of Neuropathic Pain

Chronic pain is a dominant health problem. It is the most common reason to seek a physician's care with nearly 40 million office visits scheduled annually. Pain costs the U.S over $100 billion each year in health care costs and lost productivity (NIH Guide, Vol. 24, 1995). Chronic pain is the most difficult pain to treat, especially chronic pain that follows nerve injury, known as neuropathic pain. These neuropathic pain syndromes include deafferentation pain, diabetic, cancer and ischemic ne
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Implantable Biotelemetry System for Preterm Labor and Fetal Monitoring (ARC-14280)

Abstract: The Fetal Treatment Center (FTC) at UC San Francisco has developed a revolutionary surgical procedure to treat fetuses suffering from diaphragmatic hernia, a condition in which a hole in the diaphragm allows internal organs to shift from the abdominal cavity into the chest cavity.
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Rapid Generation of Pulse Flash Free Radicals to Kill Human Cancer Cells, Recycle Human Urine, and Mineralize Organic Chemicals in Wastewater by Using Metallic Cations and Hydrides

Description This invention relates to use the reactions between cationic metals and hydrides to generate free radicals and undergo subsequent oxidation to destroy organic chemicals and kill human cancer cells. Applications The inventors have demonstrated the following novel applications of the processes described herein which generate free radicals, including, but not limited to: Industrial organic wastewater treatment Recycling of human urine Killing human cancer cells Main Advantages
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Human Consumable Essential Oil Highly Toxic to Pest Tephritid Fruit Flies

Description We discovered that an essential oil and its constitutes are very toxic to pest tephritid fruit flies including Ceratitis capitata (Wiedemann) (Mediterranean fly), Bactrocera dorsalis (Hendel) (oriental fly) and B. cucurbitae (Cocquillett) (melon fly). Pest tephritid fruit flies directly damage crops and are quarantine species. The insecticidal action of the essential oil or its constitutes is very fast (lethal time of 90% population, 15-38 min) and follows a very steep dose-response
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Method and Device for Biochemical Sensing

Description Medical diagnostics using chemical sensing of antibody, antigens, DNA fragments and other biochemicals are addressed using semiconductor sensors and supportive biochemical processes. This successful marriage of semiconductor sensors and biochemistry address a diversity of potential biochemical diagnostics and measurements including detection and quantification of human, animal and plant diseases. Target biochemical molecules attach specifically to one or more preselected, sensor-atta
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Laser/Fiber Optic, Piezoelectric, Scanning Confocal Microscope

Description The design includes piezoelectric bimorph bender elements that scan the aperture in the confocal image plane to create a compact, relatively inexpensive, rugged confocal microscope which can be remotely deployed on a fiber optic cable and miniaturized for in situ analysis. The scanning unit for the microscope allows applications not possible with the conventional confocal microscope. Applications Biological and medical research on tissue samples in cytological and neurological appli
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Monoclonal Antibody DZ-1 for M. leprae (leprosy bacillus)

Description The DZ-1 monoclonal antibody detects specific antigen for the leprosy bacillus, Mycobacterium leprae. The recognized antigen is Phenolicglycolipid-I (PG-I). The monoclonal antibody reacts with the innermost sugar (0-3-methylrhamnose) of the trisaccharide antigenic component of the PG-I. Applications Leprosy, also known as Hansen's disease, is an infectious disease commonly manifested by cutaneous and neural lesions. The antibody response of the DZ-1 monoclonal antibody to the PG-I a
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Detection and Identification of Enteric Pathogens

Description Two pairs of DNA primers have been designed to be used in the Polymerase Chain Reaction System (PCR) to consecutively detect both Salmonella and Shigella. The method used to detect the bacteria involves isolating and purifying nucleic acids and using the primers in the PCR system to amplify Salmonella and Shigella-specific sequences which are then identified using gel electrophoresis. Applications The current method can be used for the detection of enteric pathogens in environmental
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Serotype-Specific Probes and Nucleotide Sequences of Listeria Monocytogenes Cluster IIB (4b-4d-4e)

Description Two genes which are unique to serotype cluster IIB (4b - 4d - 4e) have been characterized for Listeria monocytogenes. Serotype 4b is known to be the primary pathogenic species causing listeriosis. The serotype-specific region has been sequenced in its entirety from both strands. The sequences themselves correspond to novel genes, which are involved in the expression of unique surface antigens of L. monocytogens cluster IIB. In tests of 150 strains of Listeria, the unique sequence cou
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Silkworm/Baculovirus Expression System for Production of MSP1 Malaria Vaccine

Description Malaria is a tropical disease that annually infects 200 million people in developing countries, with an estimated 2 -2.5 million people, mostly children, that die of this disease. Globally, over one billion people are at risk of contracting malaria infections. Malaria has continued to exert a tremendous toll on the health, social and economic well being of affected countries. Today, most experts agree that an efficacious and cost effective vaccine will be the key to control this drea
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Optimization of Expression Construct and Purification Protocols for a MSP1.42 Malaria Vaccine

Description The invention consists of two parts which both contribute to an enhanced feasibility of commercial production of this potential malaria vaccine: (1) optimization of the MSP1.42 gene construct for enhanced levels of production in the baculovirus/insect cell culture expression system, and (2) development of a simplified, non-denaturing purification scheme which enhances recovery and purity of the MSP1.42 polypeptide. Applications Malaria vaccine Main Advantages Approximately 5-10
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Baculovirus Produced P. falciparum Vaccine

Description The technology covers the construct consisting of the baculovirus polyhedron promoter, the yeast flg5 leader sequence, and the p42 MSP1 sequence with the transmembrane domain deleted cloned at the University of Hawaii from the Uganda-Palo Alto isolate of P. falciparum. The patent application also considers the production of other, related p42 constructs based on allelic and variant sequences expressed by other isolates of P. falciparum. Applications Malaria vaccine. Main Advant
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Taccalonolide Microtubule-Stabilizing Agent

Description This new microtubule-stabilizing agent is an effective cytotoxin against many cancer cells in vitro. Bioassay-directed purification of the crude lipophilic extract yielded a steroid with paclitaxel-like microtubule-stabilizing activity. Non-transformed cells treated with the agent exhibited interphase arrest and extensive microtubule bundling. Many of the microtubule bundles were located in the cell periphery, apparently nucleated independent of the microtubule-organizing center. The
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Method of Visualizing Chromosomes of Somatic Cells that Do Not Divide

Description Examination of mitotic chromosomes is essential for cytogenetic research, in particular for the chromosomal mapping of the genes responsible for some genetic disorders and cancers linked with the chromosomal alterations. Till today, no methods have been developed to visualize mitotic chromosomes of postmitotic cells (e.g., neurons). Chromosomes become visible only during cell division. Adult brain cells never divide and therefore their chromosomes have never been examined. This is a
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Diagnosis and Treatment of Pre-Term Labor

Description Pre-term birth is a serious medical problem in the U.S. with almost half a million babies born prematurely each year. In spite of all recent medical advancements, the premature birth rate continues to climb and the reasons remain unknown. Pre-term birth is the leading cause of neonatal death, although even the smallest babies have a better chance of surviving than in the past. Babies born prematurely often have many health problems, some of which persist into adult life. Despite a ma
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Monoclonal Antibodies against Human Flightless-I Protein

Description The Flightless-I Protein was originally identified in the vinegar fly Drosophila but it is conserved in most multicellular organisms, including humans. Studies have shown that mutations in this gene result either in abnormal muscle function or in arrested development and embryonic lethality. The protein sequence indicates that Flightless-I may be a regulator of the cytoskeleton and may have an important role during cell division. Adding to its essential role in early development,
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Novel Fluorescent Nanosensor Proteins

Description Two innovative sensor-protein platforms have been developed by UH researchers. These sensors are designed for highly sensitive fluorescence resonance energy transfer (FRET)-based molecular assays. Both sensors are suited for homogeneous assays, with one of the sensors designed for noncompetitive assays, and the other for competitive assays. A wide range of molecular recognition domains (e.g., peptide epitopes) can be incorporated into these nanosensor proteins using recombinant DNA t
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A Novel Optical Sensor Configuration

Description A novel optical-fiber sensor configuration has been developed for use in parallel bioreactor arrays and other potential applications. A major advantage of this configuration (as opposed to the commonly applied backscattering configuration) is that it minimizes or eliminates the effect of exterior light absorption/scattering on the sensor measurement, while allowing the detector to be positioned in a way that is amenable for sensor multiplexing, enabling substantial reduction in the c
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Urine Preservative with Combined Antibacterial and Antioxidant Properties (MSC-22695)

Abstract: The Urine Preservative with Combined Antibacterial and Antioxidant Properties preserves urine analytes at ambient temperatures for extended periods of time. The product does not require refrigeration and does not alter urinary pH. It also eliminates or reduces the primary causes of analyte destruction in urine and bacterial contamination and oxidation. The new preservative exhibits antimicrobial and antioxidant properties by combining a known topical anti-infective agent with a known
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Fiber Optic Device for Sensing the Presence of Gas

Technology Description A fiber-optic device for sensing the presence of a gas in an environment is provided. The device comprises a light source for directing a light beam to a layer system having a first surface and a second surface opposite the first surface. The first surface is exposable to the light beam and the second surface is exposable to the environment. A first light portion encounters and reflects from the first surface at an angle of incidence free from optical wave guide resonance
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Humanized Model for Spontaneous Autoimmune Myocarditis

Description: Myocarditis is a major cause of sudden death in children and young adults and is often a precursor of dilated cardiomyopathy, a common indication for cardiac transplantation. Myocarditis presents a challenging clinical problem because it is hard to diagnose and the treatment options are limited. It has been widely thought that autoimmune myocarditis is of infectious etiology, and to date, all animal models have involved experimental induction of disease, either by infection wi
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Mouse Obesity Model with Modified Leptin Receptor

Description: The hormone leptin regulates food intake, metabolic rate and endocrine function. Disruption of leptin or its receptor molecule abolishes normal regulatory mechanisms, leading to obesity and endocrine dysfunction. The leptin receptor activates certain signal transduction pathways that mediate its normal roles in the cell. The inventors have discovered that certain signaling pathways of the leptin receptor play a role in control of body weight, while distinct pathways control ot
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Insulin Receptor Substrate Knockout Mice

Description: The IRS proteins are the downstream substrates of the insulin receptor in the early steps of the signal transduction pathways triggered by insulin’s binding to its receptor. Insulin Receptor Substrate-1 (IRS-1) is a substrate for tyrosine phosphorylation by the activated insulin receptor B subunit following receptor activation. The IRS-2 protein is part of a family of proteins which are substrates for the insulin receptor, as well as the IL-4 and IL-15 receptors.
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MCK Cre Mice

Description: This technology is an improvement to the cre-lox system for making targeted gene knockouts in mice. Clinical & Commercial Utility: The available technology features a line of mice harboring the Cre DNA recominbase gene under the control of the muscle creatine kinase promoter. These mice can be used to create muscle-specific knockouts using the cre-lox system. Patent Status: The creation of this mouse line, and a description of its us
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Insulin Receptor Knockout Mice

Description: The Insulin/IGF family of ligands and receptors controls mammalian growth, metabolism and reproduction, and insulin signaling at the insulin receptor is implicated in important signal transduction pathways. Interactions at this receptor can either amplify, diversify or terminate insulin action. One fruitful strategy for studying insulin receptor action is the creation of mouse models in which the gene encoding the insulin receptor has been ablated or knocked out.
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Melanocyte Concentrating Hormone Knockout Mice.

Description: Melanocyte concentrating hormone (MCH) is a cyclic peptide found in fish and mammals. Although involved in regulation of color change in fish, it is localized in the ventral aspect of the Zona Incerta and the lateral hypothalamus in mammals, and was suspected to play a role in complex mammalian behaviors. MCH has been shown to regulate eating behavior in mammals. Specifically, MCH is known to stimulate feeding in rodents, while animals lacking the MCH gene are lean. This molec
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Diagnosing and Treating Retinal Vascular Permeability

Description: Diabetic retinopathy (DR) is the leading cause of vision loss in working adults. Although its incidence and progression can be reduced by intensive glycemic and blood pressure control, nearly all patients with type 1 diabetes mellitus and over 60% of those with type 2 diabetes eventually develop retinal microvascular abnormalities, and 20% to 30% of these patients advance to active proliferative diabetic retinopathy (PDR) and/or diabetic macular edema. Although surgical option
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Markers for Pancreatic Cancer and Cell Growth

Description: Pancreatic cancer has one of the highest mortality rates of any malignancy, and the 5-year-survival rate of patients is 4%. Approximately 28,000 patients with pancreatic cancer are diagnosed each year, and nearly all of these patients will die of their disease. The poor prognosis of this malignancy is a result of the difficulty of early diagnosis and poor response to current therapeutic methods. In particular, no tumor markers are currently identified that allow reliable scree
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Methods of Diagnosing Cardiovascular Disease

Cardiovascular diseases like atherosclerosis are major causes of mortality and often occur as a complication of diabetes. Vascular dysfunction and atherosclerosis parallel the progression of diabetes and may be accelerated by factors such as hyperglycemia in individuals whose disease is poorly controlled. The inventions are based on the inventors’ discovery of single nucleotide polymorphisms (SNPs) in two genes that correlate with increased risk of cardiovascular disease. In one invention (JDP
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Diagnosing and Treating Diabetic Retinopathy

Description: Diabetic retinopathy (DR) is the leading cause of vision loss in working adults. Although its incidence and progression can be reduced by intensive glycemic and blood pressure control, nearly all patients with type 1 diabetes mellitus and over 60% of those with type 2 diabetes eventually develop retinal microvascular abnormalities, and 20% to 30% of these patients advance to active proliferative diabetic retinopathy (PDR) and/or diabetic macular edema. Although surgical option
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Methods of Diagnosing Cardiovascular Disease

Cardiovascular diseases like atherosclerosis are major causes of mortality and often occur as a complication of diabetes. Vascular dysfunction and atherosclerosis parallel the progression of diabetes and may be accelerated by factors such as hyperglycemia in individuals whose disease is poorly controlled. The inventions are based on the inventors’ discovery of single nucleotide polymorphisms (SNPs) in two genes that correlate with increased risk of cardiovascular disease. In one invention (JDP
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Methods of Diagnosing Renal Disease

Description: Diabetic kidney disease, also known as diabetic nephropathy, will develop in 25-40% of individuals with Type 1 diabetes mellitus, often leading to early death. The development of diabetic nephropathy generally consists of progression from normoalbuminuria (normal Urinary Albumin Excretion, or UAE) through three successive clinical stages: microalbuminuria (MA), characterized by a small increase in UAE; overt proteinuria, characterized by abundant UAE and the beginnings of rena
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Retinal Imaging System

Description: The invention overcomes certain problems inherent in traditional systems for retinal imaging through pupils that have not undergone pharmacological dilation (mydriasis). This is of particular importance in teleophthalmology, where pupil dilation can be a barrier for retinal eye examinations, particularly in patients where natural pupil dilation in low ambient light is small. To reduce haze and glare in the image of the retina, it is necessary to keep the retinal illuminating
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Predictors of Renal Disease

Description: Diabetic kidney disease, also known as diabetic nephropathy, is an important cause of excess morbidity and premature mortality in individuals with Type 1 diabetes mellitus, 25% to 40% of whom will ultimately develop diabetic nephropathy. The most serious long-term effect of diabetic nephropathy is kidney failure leading to end stage renal disease (ESRD), a condition in which there is a permanent and almost complete loss of kidney function. Other causes of ESRD include high blo
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Type 2 Diabetes Mellitus Gene

Description: Type 2 diabetes mellitus is a highly prevalent metabolic disease characterized by hyperglycemia (high blood sugar) as a result of defective insulin action which manifests as insulin resistance, defective insulin secretion, or both. Due to the severity of some of its complications, early detection of diabetes is critical. The majority of patients with diabetes have Type 2 diabetes, the non-insulin dependent form. The invention is based on the discovery of a gene correlated with
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Protein Kinase C Monocyte Assay

Description: Recent studies have shown that hyperglycemia induces de novo diacylglycerol synthesis and activates Protein Kinase C (PKC), which leads to many kinds of vascular abnormalities (e.g. abnormalities of the retina or cardiovascular tissues). A means to measure PKC activity in vascular tissues is desired, but such samples are not easy to repeatedly obtain, especially from diabetic patients. The invention stems from the discovery that PKC activity in vascular tissues is correlated w
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Repeat Polymorphism Gene and Uses Thereof

Description: This invention is based on the finding that there is a link between risk for non-insulin-dependent diabetes mellitus (NIDDM) and frataxin, a gene relating to an ataxia disorder. This disorder is caused by a long GAA expansion in this gene, creating a defective protein. However, patients having an intermediate expansion of GAA nucleotides in this gene do not have the ataxia phenotype but instead are at higher risk for developing NIDDM, making this a useful gene for diagnostics
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Antigen Associated with Type I Diabetes Mellitus

Description: This invention relates to a protein, designated PM-1, that is expressed in human pancreatic islet cells. Autoantibodies to this protein have been found in serum of some prediabetic individuals who later developed diabetes, while such autoantibodies have not been found in serum of nondiabetic individuals. This evidence suggests that these autoantibodies are correlated to the development of diabetes, and possibly to a predisposition to the disease. This leads to possible strateg
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Diabetogene RAD

Description: This invention relates to a protein whose expression is correlated with certain diabetes disease states, and may therefore play a key role in the etiology of the disease, or may be a marker that is symptomatic of certain forms of diabetes. The Rad gene encodes a novel member of the ras/GTPase gene family, which shares a 45-55% homology at the nucleotide level and a 33% homology at the amino acid level in the GTPase domain with other members of this family. The expression of Ra
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Improving Glycemic Control

Description: In differential gene expression studies of human tissue, the inventors have found that expression of genes regulated by the transcription factor SRF (serum response factor) is increased in individuals with type 2 diabetes. SRF is a highly conserved transcription factor that binds the CArG box, and it is known that knockouts of SRF are lethal. Genes whose transcription is activated by SRF were found to have elevated expression in human subject with insulin resistance compared t
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ARNT2: a Novel Target for Diabetes

Description: The invention is based on the discovery of new targets not previously known to be linked to diabetes. Microarray analysis of human pancreatic islets from people with Type 2 diabetes compared to controls with normal glucose tolerance showed that the transcription factor aryl hydrocarbon nuclear receptor translocator (ARNT) was down-regulated in the diabetic individuals. This factor was expressed in all of the control individuals but expression was not detected in microarray an
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ARNT: a Novel Target for Treatment of Diabetes

Description: The invention is based on the discovery of a new target, not previously known to be linked to diabetes. Microarray analysis of human pancreatic islets from people with Type 2 diabetes compared to controls with normal glucose tolerance showed that the transcription factor aryl hydrocarbon nuclear receptor translocator (ARNT) was down-regulated in the diabetic individuals. This factor was expressed in all of the control individuals but expression was not detected in microarray a
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SKN-1 and Oxidative Stress Resistance

Description: The transcription factor SKN-1 specifies formation of the feeding and digestive system during early stages of C. elegans embryogenesis. The inventor and his colleagues have shown that in larvae and adults SKN-1 regulates the Phase 2 detoxification response, a conserved defense against oxidative stress, and that skn-1 is important for stress resistance and longevity. In addition, the investigators have found that glycogen synthase kinase-3 (GSK-3) is a regulator of SKN-1. C. el
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Insulin Related Transcription Factor

Description: The invention pertains to the identification and cloning of the gene encoding a previously-unknown beta-cell specific transcription factor controlling insulin gene expression. There are known to be at least three critical enhancer elements that regulate expression of the gene encoding insulin, and the transcription factors that bind two of these elements have previously been identified. Although the existence of the third glucose responsive transcription factor has been implic
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Methods of Modulating Angiogenesis

Description: The invention is based on the finding that the delta isoform of Protein Kinase C can modulate pathways leading to angiogenesis or cell growth. Specifically, PKC-delta was found to regulate endothelial cell differentiation into tubes. Agonists of PKC-delta can be used to promote tube formation to reduce permeability, while PKC-delta antagaonists can be used to prevent or reduce tube formation, thereby inhibiting angiogenesis. The inventions can be practiced using known antagoni
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Methods for Identifying Compounds for Treatment of Insulin Resistance

Description: This invention arises from the discovery that aspirin’s effect on insulin metabolism is a result of its action against the IKK-beta kinase pathway. IKK-beta activation causes insulin resistance under conditions of obesity, high fat diet and lipid infusion. Aspirin (salicylate) inhibits the catalytic subunit of IKK-beta and this enhances signaling through signal transduction cascades arising from the insulin receptor. This can lead to reversal of insulin resistance. Heterozyg
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Diabetogene RAD

Description: This invention relates to a protein whose expression is correlated with certain diabetes disease states, and may therefore play a key role in the etiology of the disease, or may be a marker that is symptomatic of certain forms of diabetes. The Rad gene encodes a novel member of the ras/GTPase gene family, which shares a 45-55% homology at the nucleotide level and a 33% homology at the amino acid level in the GTPase domain with other members of this family. The expression of Ra
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Insulin Receptor Substrate Portfolio

This portfolio of issued patents covers several proteins (and the genes encoding them) that are the downstream substrates of the insulin receptor in the early steps of the signal transduction pathways triggered by insulin’s binding to its receptor. Patent No. 5,260,200 covers DNA encoding the IRS-1 protein and Patent No. 5,621,075 covers the IRS-1 protein itself, that is a substrate for tyrosine phosphorylation by the activated insulin receptor B subunit, following receptor activation. Patent
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Insulin Receptor Substrate Knockout Mice

Description: The IRS proteins are the downstream substrates of the insulin receptor in the early steps of the signal transduction pathways triggered by insulin’s binding to its receptor. Insulin Receptor Substrate-1 (IRS-1) is a substrate for tyrosine phosphorylation by the activated insulin receptor B subunit following receptor activation. The IRS-2 protein is part of a family of proteins which are substrates for the insulin receptor, as well as the IL-4 and IL-15 receptors.
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Treatment of Metabolic Disorders with Salicylates

Description: Inflammation participates in the pathogenesis of insulin resistance, type 2 diabetes and cardiovascular disease, and weight gain and obesity are known to be accompanied by activation of at least two inflammatory pathways in adipose tissue and liver: the JNK pathway and the NF-êB pathways. Both pathways increase the production of proinflammatory cytokines and chemokines and promote recruitment of macrophages to adipose tissue. The salicylate family of compounds have long been
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Obesity and Body Fat Distribution

Description: Obesity is an epidemic health problem worldwide that impacts on the risk and prognosis of many diseases, including diabetes, cardiovascular disease, hyperlipidemia, and cancer. However, not all obese patients have the same risk of developing these disorders. Individuals with peripheral obesity, i.e., fat distributed subcutaneously in the gluteofemoral region, are at little or no risk of the common medical complications of obesity, whereas individuals with central obesity, i.e.
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Methods and Compositions for Inducing Brown Adipogenesis

Description: The most common fat cells are white adipose tissue (WAT) cells, which have a thin ring of cytoplasm surrounding a lipid or fat droplet. WAT is found underneath the skin and provides heat insulation, cushioning against shock and jarring, and energy reserves. An average lean person has roughly 20 to 40 billion WAT cells, and an obese person can have up to ten times more WAT than the average lean person. The less common fat cells are the brown adipose tissue (BAT) cells. Energy e
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Methods and Compositions for Modulating Necdin Function

Description: The most common fat cells are white adipose tissue (WAT) cells, which have a thin ring of cytoplasm surrounding a lipid or fat droplet. WAT is found underneath the skin and provides heat insulation, cushioning against shock and jarring, and energy reserves. An average lean person has roughly 20 to 40 billion WAT cells, and an obese person can have up to ten times more WAT than the average lean person. The less common fat cells are the brown adipose tissue (BAT) cells. Energy e
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Methods of Diagnosis and Treatment of Metabolic Disorders

Description: The invention is based on the discovery that two proteins within a specific family play key roles in the pathogenesis of metabolic disorders such as Type 2 diabetes and obesity. The family of proteins are the sirtuins, Class III histone deacetylases (HDACs) which are known to play roles in gene silencing and DNA repair, and which also have been implicated in determination of lifespan in model organisms. The inventors have discovered that one member of the sirtuin family can pr
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Methods and Compositions for Modulating Adipocyte Function

Description: The most common fat cells are white adipose tissue (WAT) cells, which have a thin ring of cytoplasm surrounding a lipid or fat droplet. WAT is found underneath the skin and provides heat insulation, cushioning against shock and jarring, and energy reserves. An average lean person has roughly 20 to 40 billion WAT cells, and an obese person can have up to ten times more WAT than the average lean person. The less common fat cells are the brown adipose tissue (BAT) cells. Energy e
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Regulation of Eating Behavior

Description: Melanin concentrating hormone (MCH) is a cyclic peptide found in fish and mammals. Although involved in regulation of color change in fish, it is localized in the ventral aspect of the Zona Incerta and the lateral hypothalamus in mammals, and was suspected to play a role in complex mammalian behaviors. This invention is based on the discovery that MCH regulates eating behavior in mammals. Specifically, MCH has been shown to stimulate feeding in rodents, while animals lacking t
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Mouse Obesity Model with Modified Leptin Receptor

Description: The hormone leptin regulates food intake, metabolic rate and endocrine function. Disruption of leptin or its receptor molecule abolishes normal regulatory mechanisms, leading to obesity and endocrine dysfunction. The leptin receptor activates certain signal transduction pathways that mediate its normal roles in the cell. The inventors have discovered that certain signaling pathways of the leptin receptor play a role in control of body weight, while distinct pathways control ot
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Melanocyte Concentrating Hormone Knockout Mice.

Description: Melanocyte concentrating hormone (MCH) is a cyclic peptide found in fish and mammals. Although involved in regulation of color change in fish, it is localized in the ventral aspect of the Zona Incerta and the lateral hypothalamus in mammals, and was suspected to play a role in complex mammalian behaviors. MCH has been shown to regulate eating behavior in mammals. Specifically, MCH is known to stimulate feeding in rodents, while animals lacking the MCH gene are lean. This molec
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Compositions and Methods for Treating Diabetic Eye Disease

Description: Diabetic retinopathy (DR) is the leading cause of vision loss in working adults. Nearly all patients with type 1 diabetes mellitus and over 60% of those with type 2 diabetes eventually develop retinal microvascular abnormalities, and 20% to 30% of these patients advance to active proliferative diabetic retinopathy (PDR) and/or diabetic macular edema. Increased retinal vascular permeability (RVP) is a primary cause of diabetic macular edema and a characteristic finding in PDR.
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Diagnosing and Treating Retinal Vascular Permeability

Description: Diabetic retinopathy (DR) is the leading cause of vision loss in working adults. Although its incidence and progression can be reduced by intensive glycemic and blood pressure control, nearly all patients with type 1 diabetes mellitus and over 60% of those with type 2 diabetes eventually develop retinal microvascular abnormalities, and 20% to 30% of these patients advance to active proliferative diabetic retinopathy (PDR) and/or diabetic macular edema. Although surgical option
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Methods for Treatment of Rheumatoid Arthritis

Description: Rheumatoid arthritis affects approximately 1% of the world’s population, and is a significant cause of morbidity. Current therapies for rheumatoid arthritis are based on suppressing the immune system, either by interference with the function of lymphocytes or cytokines. However, until a cure for rheumatoid arthritis is found, other molecules and pathways need to be considered as potential therapeutic targets. The invention is based on the discovery that a peptide neurotransm
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Treatment of Cardiovascular Disorders with Salicylates

Description: Inflammation participates in the pathogenesis of insulin resistance, type 2 diabetes and cardiovascular disease, and weight gain and obesity are known to be accompanied by activation of at least two inflammatory pathways in adipose tissue and liver: the JNK pathway and the NF-êB pathways. Both pathways increase the production of proinflammatory cytokines and chemokines and promote recruitment of macrophages to adipose tissue. The salicylate family of compounds have long been
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Enhancing Stem Cell Mobilization

Description: This invention provides a potentially novel approach to enhancing the efficiency of stem cell mobilization, to improve the effectiveness of stem cell transplantation therapies. Hematopoietic stem cells (HSCs) can functionally regenerate an ablated hematopoietic system, and bone marrow and peripheral blood progenitor cell transplantation are therapeutic approaches that are increasingly used for the treatment of leukemia, lymphoma, multiple myeloma, breast and ovarian cancers, a
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Diagnosing and Treating Diabetic Retinopathy

Description: Diabetic retinopathy (DR) is the leading cause of vision loss in working adults. Although its incidence and progression can be reduced by intensive glycemic and blood pressure control, nearly all patients with type 1 diabetes mellitus and over 60% of those with type 2 diabetes eventually develop retinal microvascular abnormalities, and 20% to 30% of these patients advance to active proliferative diabetic retinopathy (PDR) and/or diabetic macular edema. Although surgical option
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Methods of Modulating Angiogenesis

Description: The invention is based on the finding that the delta isoform of Protein Kinase C can modulate pathways leading to angiogenesis or cell growth. Specifically, PKC-delta was found to regulate endothelial cell differentiation into tubes. Agonists of PKC-delta can be used to promote tube formation to reduce permeability, while PKC-delta antagaonists can be used to prevent or reduce tube formation, thereby inhibiting angiogenesis. The inventions can be practiced using known antagon
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Methods of Modulating Fibrosis

Description: The invention features a novel approach to the modulation of the cellular pathways leading to fibrosis and extracellular matrix deposition. Connective tissue growth factor (CTGF) is known to be a potent activator of fibrosis, angiogenesis and extracellular matrix production. The invention is based on the finding that a different growth factor regulates CTGF activity through a particular signal transduction pathway. Decreasing the activity of this other growth factor, or of key
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Treatment of Permeability Failure

Description: Peritoneal dialysis is used to treat patients having renal failure. The dialysis fluid used in such treatment contains very high levels of glucose, in order to cause the osmotic extraction of toxins from the blood; however, these levels of glucose appear to damage the cells lining the peritoneum, resulting in decreased permeability between the dialysis fluid and the blood. The invention is based on the discovery that protein kinase C (PKC) is responsible for the damaging effec
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Diabetogene RAD

Description: This invention relates to a protein whose expression is correlated with certain diabetes disease states, and may therefore play a key role in the etiology of the disease, or may be a marker that is symptomatic of certain forms of diabetes. The Rad gene encodes a novel member of the ras/GTPase gene family, which shares a 45-55% homology at the nucleotide level and a 33% homology at the amino acid level in the GTPase domain with other members of this family. The expression of Ra
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Humanized Model for Spontaneous Autoimmune Myocarditis

Description: Myocarditis is a major cause of sudden death in children and young adults and is often a precursor of dilated cardiomyopathy, a common indication for cardiac transplantation. Myocarditis presents a challenging clinical problem because it is hard to diagnose and the treatment options are limited. It has been widely thought that autoimmune myocarditis is of infectious etiology, and to date, all animal models have involved experimental induction of disease, either by infection wi
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Methods of Generating Insulin-Producing Cells

Description: A promising avenue of investigation for treatment of diabetes is that of islet cell transplantation. A variety of strategies are under investigation for the introduction into diabetic patients of functioning pancreatic islet cells, which can produce insulin and overcome the diabetic phenotype. Although some results have been encouraging, there have been no long-term successes with islet transplantation. Problems encountered to date include the need for patients to take immunos
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Encapsulated Pancreatic Cell Islet Products

Description: The efficacy of islet cell transplantation as a therapeutic strategy for diabetes has been demonstrated in humans by the Edmonton Protocol, but a number of obstacles remain for wide scale application. Problems encountered to date include the need for patients to permanently take immunosuppressive drugs, which may entail serious side effects. Microencapsulation has been used for full or partial protection of transplanted islets from immune rejection, however, the use of microca
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Beta Cell Growth and Differentiation

Description: Significant efforts are currently being directed at the regeneration of functional pancreatic islets from beta-cells, as a possible treatment for Type 1 diabetes. However, the factors that stimulate the growth of beta-cells are not yet well understood. The basis for this invention is the finding that beta-cell replication occurs even in the postdevelopmental state of beta-cell growth. It appears that a coordinated and regulated process of beta-cell replication that is analogou
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Methods of Modulating Beta-Cell Growth and Function

Description: The invention is based on the discovery that melanin concentrating hormone (MCH), a naturally-occurring peptide hormone known to be involved in feeding behavior and certain neurological functions, can also modulate pancreatic endocrine function and/or growth. The inventors have discovered that MCH and its receptor are expressed in mouse and human pancreatic islets. It has further been shown that overexpression of MCH in transgenic mice results in increased plasma insulin conc
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Insulin Related Transcription Factor

Description: The invention pertains to the identification and cloning of the gene encoding a previously-unknown beta-cell specific transcription factor controlling insulin gene expression. There are known to be at least three critical enhancer elements that regulate expression of the gene encoding insulin, and the transcription factors that bind two of these elements have previously been identified. Although the existence of the third glucose responsive transcription factor has been implic
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Method of Making Pancreatic Islet Cells

Description: A major, very promising avenue of investigation for treatment of diabetes is that of islet cell transplantation. A variety of strategies are under investigation for the introduction into diabetic patients of functioning pancreatic islet cells, which can produce insulin and overcome the diabetic phenotype. Although some results have been encouraging, there have been no long-term successes with islet transplantation. Problems encountered to date include the need for patients to
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Immunologically Privileged Cells and Uses Thereof

Description: A major, very promising avenue of investigation for treatment of diabetes is that of islet cell transplantation. A variety of strategies are under investigation for the introduction into diabetic patients of functioning pancreatic islet cells, which can produce insulin and overcome the diabetic phenotype. Although some results have been encouraging, islet transplantation has only recently begun to be explored in human clinical trials. Problems encountered to date relate to the
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Improving Glycemic Control

Description: In differential gene expression studies of human tissue, the inventors have found that expression of genes regulated by the transcription factor SRF (serum response factor) is increased in individuals with type 2 diabetes. SRF is a highly conserved transcription factor that binds the CArG box, and it is known that knockouts of SRF are lethal. Genes whose transcription is activated by SRF were found to have elevated expression in human subject with insulin resistance compared t
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ARNT2: a Novel Target for Diabetes

Description: The invention is based on the discovery of new targets not previously known to be linked to diabetes. Microarray analysis of human pancreatic islets from people with Type 2 diabetes compared to controls with normal glucose tolerance showed that the transcription factor aryl hydrocarbon nuclear receptor translocator (ARNT) was down-regulated in the diabetic individuals. This factor was expressed in all of the control individuals but expression was not detected in microarray ana
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Treatment of Metabolic Disorders with Salicylates

Description: Inflammation participates in the pathogenesis of insulin resistance, type 2 diabetes and cardiovascular disease, and weight gain and obesity are known to be accompanied by activation of at least two inflammatory pathways in adipose tissue and liver: the JNK pathway and the NF-êB pathways. Both pathways increase the production of proinflammatory cytokines and chemokines and promote recruitment of macrophages to adipose tissue. The salicylate family of compounds have long been
...

Methods of Diagnosis and Treatment of Metabolic Disorders

Description: The invention is based on the discovery that two proteins within a specific family play key roles in the pathogenesis of metabolic disorders such as Type 2 diabetes and obesity. The family of proteins are the sirtuins, Class III histone deacetylases (HDACs) which are known to play roles in gene silencing and DNA repair, and which also have been implicated in determination of lifespan in model organisms. The inventors have discovered that one member of the sirtuin family can pr
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ARNT: a Novel Target for Treatment of Diabetes

Description: The invention is based on the discovery of a new target, not previously known to be linked to diabetes. Microarray analysis of human pancreatic islets from people with Type 2 diabetes compared to controls with normal glucose tolerance showed that the transcription factor aryl hydrocarbon nuclear receptor translocator (ARNT) was down-regulated in the diabetic individuals. This factor was expressed in all of the control individuals but expression was not detected in microarray a
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Methods of Treating or Preventing Autoimmune Conditions

Description: The innate immune system is the first line of host defense against pathogens. Innate immune cells, such as dendritic cells and macrophages, are first activated and subsequently trigger the generation of optimal adaptive immune responses. Although these cells lack the highly specific antigen receptors of B cells and T cells, they can detect pathogens by recognizing conserved motifs present in pathogen-associated components and, in turn, activate the appropriate defense pathway.
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Type 2 Diabetes Mellitus Gene

Description: Type 2 diabetes mellitus is a highly prevalent metabolic disease characterized by hyperglycemia (high blood sugar) as a result of defective insulin action which manifests as insulin resistance, defective insulin secretion, or both. Due to the severity of some of its complications, early detection of diabetes is critical. The majority of patients with diabetes have Type 2 diabetes, the non-insulin dependent form. The invention is based on the discovery of a gene correlated with
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Methods for Identifying Compounds for Treatment of Insulin Resistance

Description: This invention arises from the discovery that aspirin’s effect on insulin metabolism is a result of its action against the IKK-beta kinase pathway. IKK-beta activation causes insulin resistance under conditions of obesity, high fat diet and lipid infusion. Aspirin (salicylate) inhibits the catalytic subunit of IKK-beta and this enhances signaling through signal transduction cascades arising from the insulin receptor. This can lead to reversal of insulin resistance. Heterozyg
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Antigen Associated with Type I Diabetes Mellitus

This invention relates to a protein, designated PM-1, that is expressed in human pancreatic islet cells. Autoantibodies to this protein have been found in serum of some prediabetic individuals who later developed diabetes, while such autoantibodies have not been found in serum of nondiabetic individuals. This evidence suggests that these autoantibodies are correlated to the development of diabetes, and possibly to a predisposition to the disease. This leads to possible strategies for therapeutic
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Regional Chemotherapy with 4-Hydroperoxycyclophosphamide (Intrathecal Administration)

A method of treating human tumors is provided in which tumors are regionally treated with a cytolytic, pre-activated, bifunctional alkylating agent, namely 4-hydroperoxycyclophosphamide. The treatment is effective against a variety of tumor types within the central nervous systems. Cyclophosphamide, an alkylating agent with a broad spectrum of anti-tumor activity is actually a prodrug which, to be activated must be converted by hepatic microsomal enzymes into 4-hydrocyclophosphamide. This compou
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Establishment of a Human Cerebral CortexCell Line

This invention consists of a continuous, non-malignant, neuronal cell line, the cells of which: a) in the undifferentiated form are essentially free of branched process; b) stain positively for neurofilament protein and neurotransmitters; c) do not stain positively for glial fibrillary acidic protein; and d) in the presence of nerve growth factor differentiate into cells with long branched processes. Derivative cell lines of such cell lines are also contemplated. The cell line is useful in the s
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MIG2

A new member of the chemokine family of cytokines has been identified. These chemokines are produced by interferon-gamma stimulated monocytes. Both the human (HuMig) and marine (MuMig) genes have been identified and cloned. These proteins are members of the platelet factor 4/IL-8 cytokine family. Like other members of this family, the Mig proteins are implicated in inflammation and chemotaxis.
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Primary Culture of Olfactory Neurons

Abstract (Set) The instant primary cultures of purified olfactory neurons can be stimulated with physiological levels of odorants. The neurons of the cultures express markers characteristic of mature olfactory neurons in vivo, such as vimentin, olfactory marker protein and neuron-specific enolase Description (Set) Proposed Use (Set) The cultures are useful for screening for odorants and antagonists, as well as for biochemical and physiological studies of olfactory transduction.
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Anti-viral Target for Herpes Virus

Abstract (Set) A herpes virus proteinase has been identified and cloned from cytomegalovirus. The substrate for the proteinase, which is an assembly protein precursor, has also been identified. The proteinase is useful in an assay for identifying inhibitors as potential antiviral therapies. Such inhibitors are based upon the structure and sequence of the substrate. Description (Set) Proposed Use (Set) Assay for inhibitors of herpes virus, particularly cytomegalovirus, as potential therape
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Chronic Endothelial Cell Culture Under Flow

Abstract (Set) An in vitro method for producing a device coated with adherent cells by utilizing shear stress is provided. The device is useful in vivo such in prostheses such as artificial grafts.
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Molecular Cloning of Macrophage Nitric Oxide Synthase

We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like Neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hours after endotoxin treatment. Description (Set) Proposed Use (Set)
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Karyoplasmic Interaction Selection Strategy

Abstract (Set) Karyoplasmic Interaction Selection Strategy is a system of plasmids that assays protein-protein interactions in mammalian cells on the basis of the 2-hybrid system. Description (Set) Proposed Use (Set) This system allows testing of protein-protein interactions in living cells. It also allows mapping of interaction domains within the tested proteins
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Use of Retinoic Acid

Proliferation of retinal pigment epithelium following surgery or trauma is prevented by contacting retinal pigment epithelium cells with a therapeutic amount of a retinoic acid receptor (RAR) agonist, preferably one with specific activity for retinoic acid receptors. Preferably the RAR agonist is also a potent antagonist of AP1-dependent gene expression. Alternatively, the proliferation of retinal pigment epithelium is ameliorated with a therapeutic amount of an AP-1 antagonist, alone or in comb
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Recombinationally-targeted cloning in Saccharomyces cerevisiae artificial chromosomes

Homologous recombination techniques are used to produce yeast artificial chromosomes (YAC) containing foreign target DNA by utilizing at least three DNA segments which yield the vector. Preferred embodiments allow the production of viral DNA which is infectious.
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New Uses for Camptothecin Analogs (Shapiro - joint)

Camptothecin compounds are effective inhibitors of hemoflagellate growth and are useful in treating leishmaniasis and trypanosomiasis in livestock, other domestic animals and humans.
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Reverse Two Hybrid Systems

Methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are usefulin practicing the methods of the invention.
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Glutamate Receptor Antibodies

Sub-unit specific anti-peptide antibodies which have been developed against the predicted amino acid sequences of several rat glutamate receptor CDNAs. Description (Set) Proposed Use (Set) The ability to identify these proteins in post-mortem human tissues allows examination of the level of the changes in levels of receptor sub-types that occur in a variety of neurological and psychiatric diseases. This method is superior to auto radiography in that it is more sensitive and quantitative ,
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Method of Inhibiting Parasitic Activity

A method of inhibiting parasitic activity is disclosed in which the biosynthesis, structure and/or function of the glycosyl phosphatidylinositol (GPI) anchor of said parasite may be affected by incorporating into said GPI anchor selected analogs of myristic acid containing various heteroatoms, substituents and unsaturated bonds, including ester-containing analogs, ketocarbonyl-containing analogs, sulfur-containing analogs, double bond- and triple bond-containing analogs, aromatic moiety-containi
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Heart Simulation Software

The Heart Simulation 1 and Heart Simulation 2 programs provide a computer model of the electrical activity of the heart. More specifically, these programs allow for the simulation of cardiac arrhythmias and strategies for defibrillation. Heart Simulation 1 runs on a PC and is more suited for defibrillation studies, while Heart Simulation 2 runs on a SUN workstation and is more suited for studies of arrhythmias.
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Narp Expression and Regulation in Brain

Abstract (Set) A novel neuronal cell growth factor, neuronal-activity regulated pentraxin or NARP and its encoding cDNA sequence is provided in this invention. Narp induces dendritic neurite outgrowth, promotes neuronal migration, may modulate intermediate early gene expression in neuronal tissue and facilitates aggregation of AMPA receptors. Description (Set) Proposed Use (Set) Methods for identifying compounds which affect the formation of synaptic connections and for increasing the num
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Novel Members of the Human Immunoglobulin Superfamily that Mediate the Entry of Alphaherpesviruses into Cells ( 98025/98018)

The alphaherpesvirus subfamily includes neurotropic viruses such as human HSV-1 and HSV-2 and animal viruses such as porcine pseudorabies virus (PRV) and the bovine herpesvirus BHV-1. Viral entry into cells requires specific interactions of virion glycoproteins with cell surface heparan sulfate and with specific protein receptors. There are three proteins that can serve as receptors for alphaherpesvirus entry. These three receptors have different cellular distributions and viral specificitie
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Chondrogenic and Osteogenic Inducing Molecule ( 98063)

A new family of polypeptides, called amelogenins, with activities similar to the bone morphogenetic protein family to potentiate tissue mineralization in vivo, has been identified and characterized. These polypeptides, which are translation products of particular gene splice variants of the amelogenin gene, are useful in tissue and bone regeneration. Furthermore, the amelogenins are useful in the treatment of regeneration of dentin and in mineralization of the dental pulp, and possibly in cement
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Localization of Major Peptide Autoepitopes for Nucleosome Specific T Cells of Systemic Lupus Erythematosus (20032/99016)

Investigators at Northwestern University have identified peptides localized to distinct regions of histone proteins that are recognized by autoimmune T-cells in Systemic Lupus Erythematosus (SLE). These peptides, when administered in a mouse model of SLE, delay onset as well as significantly decrease the severity of the disease. The use of these peptides in the treatment of SLE would offer a more specific intervention by targeting the immune cells that mediate the pathogenic effects, but without
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Surface-Associated Delivery of Genes and Oligonucleotides in vivo (20071(v))

This invention describes a novel system for the controlled, efficient delivery of DNA or oligonucleotide (ON) complexes from a supporting scaffold that could be used in tissue engineering or regeneration and gene therapy. Using the proposed methods, a scaffold made of a biocompatible polymer would be implanted. Cells from the surrounding tissue would migrate across the scaffold, internalizing the gene or ON into the cells of the tissue. Multiple genes could be placed on the scaffold material to
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A New Potent Irreversible Inhibitor of y-Aminobutyric Acid Aminotransferase (21058/23058)

A promising new candidate inhibitor of the enzyme gamma-aminobutyric acid aminotransferase, critical to central nervous system disorders, has been prepared. Therapeutic potential in the treatment of epilepsy, drug addiction, pain control and neurological disorders is indicated. ADVANTAGES: In vitro studies indicate enhanced inhibition and selectivity toward the GABA-AT enzyme than present day drugs. SUMMARY: A low level of gamma-amino butyric acid (GABA) in the brain is associated with epi
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Mammalian Sterol Synthesis as a Target for Chemotherapy Against Bacteria (21061)

Recent discoveries at Northwestern University offer the opportunity to use well characterized drugs, known as statins, for a new much needed use, as antibacterial/antibiotic compounds. Thus, the present invention focuses on the use of statins as anti-bacterial drugs. Shown below is a graph demonstrating the ability of lovastatin to inhibit Salmonella infection of macrophages. BACKGROUD and SIGNIFICANCE: Bacterial infections are among the most common and deadly causes of human disease. About 7
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Cell Line Overexpressing DNA Methyltransferase

Abstract (Set) Abnormal regional increases in DNA methylation, which have potential for causing gene inactivation and chromosomal instability, are consistently found in immortalized and tumorigenic cells. Increased DNA methyltransferase activity, which is also a characteristic of such cells, is a candidate to mediate these abnormal DNA methylation patterns. We now show that, in NIH 3T3 mouse fibroblasts, constitutive overexpression of an exogenous mouse DNA methyltransferase gene results in a m
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Floxed GFRalpha-1 Mouse

Abstract (Set) Scientists at JHU have created a mouse that harbors mutations allowing for the conditional inactivation of the GFRalpha-1 protein through the use of Cre mouse lines. Description (Set) Proposed Use (Set) These mouse lines can be used to investigate the role of GDNF in neural cell development and survival and may help to shed light on the pathogenesis of Parkinson?s disease.
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Floxed Ret Mouse

Abstract (Set) Scientists at JHU have created a mouse that harbors mutations allowing for the conditional inactivation of the Ret protein through the use of Cre mouse lines. Description (Set) Proposed Use (Set) These mouse lines can be used to investigate the role of GDNF in neural cell development and survival and may help to shed light on the pathogenesis of Parkinson?s disease.
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Biomarkers for Chemo-resistance in Ovarian Cancer

Abstract (Set) Ovarian cancer is the most lethal gynecologic malignancy in the US. Although it is initially very responsive (80%) to cisplatin-based chemotherapy, 75% of patients easily develop cisplatin resistance and relapse within 2 years of primary therapy. The progression of cisplatin-resistant cancer confers poor prognosis and decreases overall survival. Several mechanisms have been implicated in cisplatin resistance, but they are not sufficient to exhaustively explain why resistance emer
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The Preparation and Application of an Adenovirus Construct to Treat Human Cancers

Abstract (Set) JHU scientists have identified a peptide derived from p55PIK, a PI 3-kinse regulatory subunit, which can effectively block the cell cycle progression of a variety of cancers. To deliver the peptide to cells, they made a replication-deficient adenovirus construct expressing the peptide. Experiments indicate that this GFP adenovirus construct inhibits the proliferation of several cultured cancer cell lines derived from human thyroid tumors and decreases the growth of tumor xenograf
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Administration of DNA Vaccines with DNA Encoding Ii-PADRE Generates Potent PADRE-specific CD4+ T-cell Immune Responses and Enhances Vaccine Potency

Abstract (Set) Various strategies have been developed for enhancing DNA vaccine potency by modifying the properties of Antigen Presenting Cells (APCs). These strategies include increasing the number of antigen-expressing Dendritic Cells (DCs); improving antigen expression, processing, and presentation in DCs; promoting DC activation and function; and enhancing DC and T cell interaction to augment vaccine-elicited T cell immune responses. JHU scientists have now developed a novel DNA vaccine enc
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Methods and Compositions for Inhibiting Angiogenesis (21064/21013/20001/96047)

Investigators at Northwestern University have discovered that pigment epithelium derived factor (PEDF), a glycoprotein secreted by a variety of normal cells, is an unusually potent endogenous inhibitor of angiogenesis, and is particularly active in the eye, the prostate, the pancreas and the kidney. Exogenous PEDF has been shown to inhibit tumor growth and increase tumor necrosis in a mouse Wilm’s tumor model and in xenograph models of human prostate cancers and neuroblastomas. The primary th
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Reactivation of Mutant p53 with a New Class of Small Molecules (23079)

Novel molecules, effective at inducing apoptosis by reactivating mutant p53, have recently been synthesized by Daniel Appella et. al. These compounds facilitate p53 protein binding to DNA and activate apoptosis in transformed cells. BACKGROUND: In healthy cells, p53 controls the integrity of cellular DNA by arresting cell proliferation, initiating cell death or mediating DNA repair in the event of DNA damage. Thus, by inducing cell death, p53 acts as a tumor suppressor by preventing a cell w
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Biodegradable, Drug-Releasing Tubes for Tissue Engineering and Regeneration (23091)

Biodegradable drug-releasing conduits for applications in tissue engineering and neuronal regeneration. These conduits are capable of delivering DNA, protein, and/or small molecules. Significance: Investigators at Northwestern have designed biodegradable nerve guidance conduits or bridges possessing multiple features for promoting nerve regeneration. These conduits serve as a mechanical support to promote and direct tissue regeneration by creating a path for neurite outgrowth and exclude inva
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Cell-specific Molecule and Method for Importing DNA into pulmonary epithelial cell nuclei (24002)

A short DNA sequence that can be placed into any gene therapy vector for efficient targeted expression in pulmonary epithelial cells. This invention has applications in the treatment of a number of pulmonary diseases and disorders such as cystic fibrosis, acute respiratory distress syndrome, and pulmonary fibrosis. Significance: This technology overcomes two of the immense difficulties in gene therapy: cell specific targeting, and gene transfer to non-dividing cells. Use of this sequence als
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Cyclic Peptides and Cyclic Depsipeptides with Antitumor Activity (24095/23053/22081)

A novel family of peptide compounds with enhanced anti-proliferative activity has been synthesized at Northwestern University. These peptide inhibitors have anticancer activity exceeding that of two known growth inhibitors. This newly identified family of compounds has been tested in human prostate, human breast cancer, melanoma and human colon cancer cell lines. ADVANTAGE: A novel class of peptide molecules that inhibit cell growth across a variety of human cancer cell lines has been creat
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Modulation of Immune Function by the Targeted Enhancement of CD154 Trimer Stability (25008)

Two lead compounds have been identified that can enhance immune function in vivo through a novel mechanism of stabilizing the CD154 trimer on the surface of activated T cells. ADVANTAGES: The compounds are the first demonstrated to enhance immune function in this way. The molecules can be applied to: Chemotherapeutic strategies -targeting and eliminating cancer cells. Standalone vaccines -protect against infectious or toxic agents. Treating infectious disease -antibiotic facilitator enha
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Arsenic Encapsulation and Triggered Release for Cancer Treatment (25053)

Novel procedures and compositions for the encapsulation and delivery of arsenic drugs. SIGNIFICANCE: Despite its long history and excellent therapy, arsenic compounds have a variety of widely appreciated toxic side effects, which currently limit their application. The invention presented here provides a novel procedure for encapsulating arsenic compounds in liposomes allowing greater exploitation of the arsenic’s beneficial characteristics while reducing its toxicity. The encapsulated comp
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Protein Kinase Targeted Therapeutics (26087)

A validated novel compound and medicinal chemistry refinement for bioavailable small molecule inhibitors of protein kinases or protein kinase-mediated cellular stress response pathways. ADVANTAGES: A small molecule p38α MAPK inhibitor presents significant therapy potential for neurodegenerative diseases and other CNS disorders where proinflammatory cytokine overproduction is a component, such as Alzheimers (AD) and related indications, Parkinson’s, multiple sclerosis, and traumatic brain
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Myosin Light Chain Kinase Inhibitor Compounds, Compositions and Related Methods of Use (26088)

A new class of small molecule therapeutic candidates with potential to address areas of disease and injury relevant to tissue barrier dysfunction, such as sepsis, lung injury, microvascular leak, Crohn's disease and related disorders, as well as multi-organ failure. ADVANTAGES: A small molecule selective inhibitor with drug-like properties suitable for treatment of disease progression and attenuation of tissue injury, with improved survival in critical care situations, where tissue barrier d
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NOS Inhibitors for Treatment of Motor Deficit Disorder (26093)

Northwestern scientists have prepared compositions that are powerful and highly selective for inhibiting neuronal NOS activity in the fetal brain and protecting fetal rabbit kits from HI-induced symptoms of cerebral palsy. The compositions appear to be nontoxic and show no detrimental cardiovascular effects. ADVANTAGE: Novel neuronal nitric oxide synthase inhibitors that significantly reduce the incidence of cerebral palsy in fetal rabbits. SUMMARY: Cerebral palsy is common in prematurely
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Improving Islet Survival by Encapsulation in Hydrogels Modified with Anti-Apoptotic Peptides (27055)

Novel in-situ hydrogels enabling islet cell encapsulation, stabilization and protection against cytokine induced cell apoptosis for improved tissue graft survival in transplantation applications. ADVANTAGES: A new and potentially general approach of encapsulating and maintaining transplant tissues with tailored hydrogels under biocompatible conditions. SUMMARY: Transplantation of pancreatic tissue is an effective method of restoring glycemic control in type I diabetic patients. Islet tra
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DNA-binding Self-assembling Peptide Amphiphile System for the Transfection of Cellular Aggregates (27116)

A self-assembling peptide system utilizing a bioactive sequence which significantly enhances transfection efficiency. ADVANTAGES: A self-assembling peptide system able to transfect aggregates of cells more completely and with higher efficiency than present day commercial reagents. The system appears useful for a variety of cells including stem cells or organiods such as the Islets of Langerhans. SUMMARY: Transfection is the process by which foreign DNA is introduced to a cell, transporte
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Flufenamic Acid for the Treatment of Chronic Pain (27153)

Oral administration of flufenamic acid (FA) has a potent analgesic effect that can be exploited for the treatment of chronic pain. Chronic pain is an invalidating condition affecting a large number of people and no pharmacologic treatment has proven completely effective so far. SIGNIFICANCE: Pain constitutes the primary reason driving people to seek health care. According to a 2006 from the National Center for Health Statistics, an estimated 76.5 million Americans report that they have had
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Layered Islet Delivery Scaffold (27154, 27090)

Northwestern University has designed a multilayered functional delivery system for islet cell transplantation. The layered polymer advances the opportunity for glycemic control in diabetic patients through tissue engineering. Key Design Innovations: Layered design isolates protein/gene delivery from cell seeding and angiogenesis Optimal mechanical and chemical properties are obtained for each functional layer Enables controlled local delivery of key islet cell growth factors (e.g. Exen
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Administration of DNA Vaccines with DNA Encoding Ii-PADRE Generates Potent PADRE-specific CD4+ T-cell Immune Responses and Enhances Vaccine Potency

Abstract (Set) Various strategies have been developed for enhancing DNA vaccine potency by modifying the properties of Antigen Presenting Cells (APCs). These strategies include increasing the number of antigen-expressing Dendritic Cells (DCs); improving antigen expression, processing, and presentation in DCs; promoting DC activation and function; and enhancing DC and T cell interaction to augment vaccine-elicited T cell immune responses. JHU scientists have now developed a novel DNA vaccine enc
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Administration of DNA Vaccines with DNA Encoding Ii-PADRE Generates Potent PADRE-specific CD4+ T-cell Immune Responses and Enhances Vaccine Potency

Abstract (Set) Various strategies have been developed for enhancing DNA vaccine potency by modifying the properties of Antigen Presenting Cells (APCs). These strategies include increasing the number of antigen-expressing Dendritic Cells (DCs); improving antigen expression, processing, and presentation in DCs; promoting DC activation and function; and enhancing DC and T cell interaction to augment vaccine-elicited T cell immune responses. JHU scientists have now developed a novel DNA vaccine enc
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DNA-binding Self-assembling Peptide Amphiphile System for the Transfection of Cellular Aggregates (27116)

A self-assembling peptide system utilizing a bioactive sequence which significantly enhances transfection efficiency. ADVANTAGES: A self-assembling peptide system able to transfect aggregates of cells more completely and with higher efficiency than present day commercial reagents. The system appears useful for a variety of cells including stem cells or organiods such as the Islets of Langerhans. SUMMARY: Transfection is the process by which foreign DNA is introduced to a cell, transporte
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Control of Human Mesothelin-expressing Tumors By DNA Vaccines

Abstract (Set) Mesothelin has been implicated as a potential ideal target antigen for the development of antigen-specific cancer immunotherapy for the control of mesothelin-expressing cancers such as ovarian cancer, mesothelioma, and pancreatic adenocarcinoma. JHU researchers utilized a DNA vaccine encoding human mesothelin to treat mice challenged with luciferase-expressing human mesothelin-expressing ovarian cancer cell line, Defb29 Vegf-Iuc/Hmeso. The therapeutic effect of the tumor challeng
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Control of Human Mesothelin-expressing Tumors By DNA Vaccines

Abstract (Set) Mesothelin has been implicated as a potential ideal target antigen for the development of antigen-specific cancer immunotherapy for the control of mesothelin-expressing cancers such as ovarian cancer, mesothelioma, and pancreatic adenocarcinoma. JHU researchers utilized a DNA vaccine encoding human mesothelin to treat mice challenged with luciferase-expressing human mesothelin-expressing ovarian cancer cell line, Defb29 Vegf-Iuc/Hmeso. The therapeutic effect of the tumor challeng
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Control of Human Mesothelin-expressing Tumors By DNA Vaccines

Abstract (Set) Mesothelin has been implicated as a potential ideal target antigen for the development of antigen-specific cancer immunotherapy for the control of mesothelin-expressing cancers such as ovarian cancer, mesothelioma, and pancreatic adenocarcinoma. JHU researchers utilized a DNA vaccine encoding human mesothelin to treat mice challenged with luciferase-expressing human mesothelin-expressing ovarian cancer cell line, Defb29 Vegf-Iuc/Hmeso. The therapeutic effect of the tumor challeng
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Generation and Characterization of a Preventive and Therapeutic HPV DNA Vaccine

Abstract (Set) Cervical cancer is one of the most common cancers in women worldwide. Persistent infection with human papillomavirus (HPV) is considered to be the etiological factor for cervical cancer. Therefore, an effective vaccine against HPV infections may lead to the control of cervical cancer. An ideal HPV vaccine should aim to generate both humeral immune response to prevent new infections as well as cell-mediated immunity to eliminate established infection or HPV-related disease. JHU sc
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Selective, Facile, Quantitative Detection of 2'-deoxyguanosine intermediates

Abstract (Set) A variety of structural modifications (lesions) are produced in DNA when the biopolymer is exposed to oxidative stress. Lesions can be genotoxic and/or cytotoxic, and are useful biomarkers. Selective, quantitative detection of lesions is useful for rapidly determining the effects of DNA damaging agents. We have developed a simple method for selectively quantifying two lesions produced from 2'-deoxyguanosine via a common reactive intermediate when DNA is exposed to oxidative stres
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Serial Assessment of Human Tumor Burdens in Mice by the Analysis of Circulating DNA

Abstract (Set) It is generally agreed that internal human xenografts provide the best animal models to study the microenvironments and metastatic processes occurring in human cancers. However, the use of such models is hampered by the logistical difficulties of reproducibly and simply assessing tumor burden. JHU researchers have developed a high-sensitivity PCR based assay for quantifying human DNA in small volumes of mouse plasma, enabling in-life monitoring of systemic tumor burden. Growth ki
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PIK3CA Exon 9 and Exon 20 Knock in Cells

Abstract (Set) PIK3CA exon 9 and exon 20 knock in cells within the MCF-10A human breast epithelial cell line. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the PIK3CA pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or chemopreventive agents
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Estrogen Receptor in Knock out (ERIK) Cells

Abstract (Set) Receptor positive non-tumorigenic human breast epithelial cell line derived from the commercially available MCF-10A human breast epithelial cell line with the p21 gene somatically deleted. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules target ER+ positive breast cancer or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic o
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K-ras Knock in Human Breast Non-tumorigenic Breast Epithelial Cells

Abstract (Set) Single oncogenic point mutation in codon 12 of K-Ras was "knocked in" to the commercially available MCF-l0A cell line and two other hTERT immortalized human breast epithelial cell lines. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules targeting the K-Ras pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chem
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Specific Delivery of a Tumor-ablating Agent by a DECApeptide to Colon and Esophageal Cancer Cell Lines as a Therapeutic Treatment

Abstract (Set) Targeted tumor therapy is commonly based on the use of antibodies directed against a specific molecule, and tagged with a tumor ablating material to deliver therapy to the target cell. Now, scientists at JHU have discovered a new method of targeting therapy to tumor cells, by using a DECApeptide that can be radioactively labeled. The DECApeptide has been shown to permanently deliver therapeutic radioisotope to colon and esophageal cancer cell lines at an efficiency that is 35 to
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Novel Anticancer Drug Leads

Abstract (Set) A novel target for cancer therapy has been discovered by JHU scientists. The drug azalanstat appears to act as an inhibitor and may be used to develop lead compounds for novel angiogenesis inhibitors. In a series of experiments, where azalanstat and small hairpin RNAs were used to knockdown the novel target, it was found that the target enzyme is required for endothelial cell proliferation. These results indicate that the enzyme is a viable target for azalanstat and other small m
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A Biotin-labeled Antibody for the Pre-clinical Diagnosis of Alzheimer?s Disease

Abstract (Set) Amyloid beta (A?is the main constituent of amyloid plaques in the brains of Alzheimer's disease patients. Research to determine the dynamic equilibrium of A?n the brain and blood is essential to understanding the pathogenesis of Alzheimer?s disease. Previous studies have shown that administration of monoclonal A?ntibody induces a rapid accumulation of A?eptide in the blood and an improvement in cognitive function. Scientists at JHU have now discovered that an injection of biotin-
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A Small Molecule Inhibitor of Glioblastomas

Abstract (Set) BACKGROUND: Mutations of the epidermal growth factor receptor (EGFR) are a possible molecular target for cancer therapy. EGFR is frequently amplified in glioblastomas and 30 to 40% of glioblastomas also express the deletion mutation EGFRvIII. This frequent oncogenic mutation provides an opportunity for identifying new anti-glioblastoma therapies. In this study, we sought small molecule inhibitors specific for cancer cells expressing EGFRvIII, using isogenic parental cells without
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Breast Milk Component Provides Protection Against Acute Respiratory Diseases

Description (Set) It has long been established that breast milk provides a protective role against acute respiratory disease. Scientists at Johns Hopkins University have identified a protective substance in breast milk that significantly decreases the risk of acute lung disease. The differences that are observed in the epidemiological studies in infants directly correlate with observations in a mouse model of acute respiratory disease. Current studies are involved in elucidating the protective
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Automate Image Analyses for Magnetic Resonance Imaging

Abstract (Set) In this disclosure, we describe methods to perform automated analyses of MRI data. We propose automated tissue segmentation and subsequent morphological and photometrical analyses. The main goal is computer assisted diagnosis (CAD) but the technique can also be applied to a wide range of MRbased researches. The proposed method consists of eight components: 1) Smar image acquisition, 2) Smart preprocessing, 3) Automated tissue segmentation, 4) Automated morphological analysis, 5)
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Variant Gene Increases Risk of Type 2 Diabetes

Description (Set) Type II Diabetes Mellitus (T2DM) represents a major global health challenge with over 170 million people affected worldwide. It is a condition in which the body fails to properly use insulin and additionally, is generally combined with relative insulin deficiency. Scientists have recently found a gene variant that increases the risk of T2DM. Variations in the gene are associated with as much as 16% of the genetic load of T2DM in specific populations. The current findings provi
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Head & Neck Squamous Cell Carcinoma an Non-Small Cell Lung Cancer Prognostics and Diagnostics

Abstract (Set) Epigenetic changes have been implicated in the pathogenesis of solid tumors and almost all types of cancers primarily via abnormal promoter hypermethylation and silencing of tumor suppressor gene expression. Epigenetic changes are changes in an organism brought about by alterations in the action of genes. JHU scientists have discovered a method to diagnose cancer, specifically, but not limited to, Head and Neck Squamous Cell Carcinoma (HNSCC) and Non-Small Cell Lung Cancer (NSCLC
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Seventy Two Gene Model for 8 Thyroid Tumor Types

Description (Set) Thyroid cancer represents 90% of all neuroendocrine malignancies with an estimated annual incidence of 122,800 cases worldwide. Currently, the most useful tool for the diagnosis of thyroid cancer is Fine-needle aspiration biopsy (FNA). This current method is extremely useful when tumors exhibit classic cytologic features, unfortunately; it also has its drawbacks in that 15-30% of FNAs fall into and ?indeterminate? or ?suspicious? category. Compounding this problem the surgical
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Twist overexpression promotes chromosomal instability in the breast cancer cell line MCF-7

Description (Set) Chromosomal instability, the continuous formation of novel chromosome mutations at a rate higher than in normal cells, is a defining characteristic of most human cancers. Twist is a basic helix-loop-helix transcription factor that is a major regulator of mesenchymal phenotypes. JHU scientists have developed a breast cancer cell line (MCF-7/Twist) that stably overexpresses human Twist. The overexpression of Twist causes an epithelial to mesenchymal-like transition (EMLT) which
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Cell Lines with Targeted Deletions of p53

Abstract (Set) Cell lines with deletions of p53 can be used for testing the specificity of drugs that target the p53 pathway. This pathway is inactivated in multiple forms of tumors. The cells lines include the following: ? HCT116 R248W/- ? DLD1 R248W ? DLD1 -/- ? RKO -/- ? SW48 -/-
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Targeted Mutagenesis of the Zebrafish Genome Through Manipulation of Helicase Activity

Description (Set) Helicase enzymes are important enzymes functioning in DNA replication, repair, recombination, and RNA transcription. RecQ helicases are a family of helicases that act to suppress homologous recombination at specific junctions. Despite the many advantages of the zebrafish as an experiment organism, there is currently no method available to induce targeted mutations in the zebrafish genome. Scientists at Johns Hopkins University are developing methods to perform targeted mutatio
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Detection of Prostate Cancer Proteins in Serum for Cancer Diagnosis with a Blood Test

Abstract (Set) There is a crucial need of discovering novel biomarkers to improve the specificity of clinical diagnosis for prostate cancer. To achieve this goal, quantitative proteomic profiling is required that can be applied in a high-throughput fashion. With the rapid developments of glycopeptide capture and stable isotope labeling technologies, JHU scientists have used mass spectrometry-based proteomic analysis (Matrix-assisted laser desorption/ionization, MALDI TOF) to quantify and identi
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Processing of Optical Coherence Tomography Signals - for both Medical and Non-Medical Applications (22044)

Optical Coherence Tomography (OCT) is the optical analog of ultrasound tomography. In OCT, an optical wave is launched into a tissue sample where it is reflected off of interfaces and where optical energy returns to a detector. These interfaces are located where the speed of light, i.e. the index of refraction, changes. One can map the intensity of the reflected light as a function of spatial dimension within the tissue to form an image. The advantage of OCT over ultrasound is that, whil
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Use of Transcranial Magnetic Stimulation (TMS) to Treat Cerebral Palsy.

Description (Set) Cerebral palsy is manifested in a wide range of muscle dysfunctions which can have a severe impact on the quality of life of the patient. Using an innovative imaging technique, JHU scientists have observed unexpected and surprising pathologies in the brain of the cerebral palsy patient that may indirectly result in deterioration of the motor cortex and reduced motor function. JHU scientists suggest that Transcranial Magnetic Stimulation (TMS) can be used to specifically target
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Radiolabeled Inhibitors for Prostate Cancer Imaging & Therapeutics

Abstract (Set) Prostate-specific membrane antigen (PSMA) is a cell surface protein that is over expressed in prostate cancer, including hormone-refractory and metastatic disease. Successful imaging of xenografts that express the prostate-specific membrane antigen (PSMA) using small animal PET and PSMA inhibitors has led to the investigation of the possibility of utilizing the more economical radionuclide 99mTc for SPECT imaging of prostate cancer. JHU scientist have prepared novel 99mTc/Re chel
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Functional Isolation of the Low-oxygenic Niche Derived Primitive Hematopoietic Stem Cells By Differential ROS Activity

Abstract (Set) A low-oxygenic niche in bone marrow limits reactive oxygen species (ROS) production, thus providing long-term protection for hematopoietic stem cells (HSCs) from ROS stress. Although many approaches have been used to enrich HSCs, none have been designed to isolate primitive HSCs located within the low-oxygenic osteoblastic-niche due to difficulties of direct physical access. Scientists at JHU show that an early HSC population that might reside in the osteoblastic niche can be fun
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Diagnosis, Prevention and Treatment of Eye Injury and Infection

Abstract (Set) According to the World Health Organization, cataracts are the leading cause of blindness in the world. Approximately 10 million cataract operations are performed each year in the world. By the year 2020 the number of blind people over 60 years of age will likely reach 54 million world-wide if current trends continue. Although it is usually treated successfully, there are a number of surgery-related complications that are of considerable clinical importance. Currently, there is no
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PET32a O-GlcNAcase-thioredoxin Expression Vector

Abstract (Set) Expression and Purification of Recombinant and Endgenous 0- GlcNAcase The human O-GlcNAcase cDNA was subcloned into the commercial expression bacterial expression vector PET32a (Novagen, Madison, WI), as was the shorter splice variant (MGEA5 s), to yield fusion proteins with thioredoxi. Both construct were confirmed by sequencing. Description (Set) Proposed Use (Set) This expression vector is used to express active O-GIcNAcase in E. coli. It produces a fusion protein betwee
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High-speed Diode for Low-cost Organic Circuitry

Abstract (Set) The Johns Hopkins University seeks a partner to commercialize a new organic diode useful for constructing complex organic electronic circuitry. The diode utilizes polymer and inorganic oxide layers to achieve high-frequency alternating current (AC) rectification, an operational requirement for constructing complex electronic circuitry, such as organic radiofrequency identification (RFID) tags. Click here for high-quality marketing materials. Description (Set) • Circuits t
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Protein Expression Markers for the Detection of Thyroid Cancer Metastasis

Description (Set) Approximately 80% of all thyroid cancers are papillary thyroid carcinomas (PTCs). Correct staging of PTC is one of the most important parts of the work up of patients for both prediction of outcome and determination of the most appropriate treatment. PTC generally has a high cure rate with standard surgical and adjuvant radioiodine treatment of neck lymph node metastases (LNM) which are found in 30-65% of initial diagnoses of PTCs. Fifteen percent of cases with LNMs also displ
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MKK4/MAP2K4 KO Lines for Drug Discovery

Description (Set) MKK4 (JNKK1, MAP2K4, and SEK1), a dual-specificity kinase gene on chromosome 17p11, is a tumor suppressor gene that is mutated in a subset of most tumor types. MKK4 protein is activated by several kinases, and active MKK4 cooperates with MKK7 to phosphorylate and thereby activate Jun NH2-terminal kinase (JNK) in the stress-activated cascade. This tumor suppressor gene is distinguished by a remarkably consistent mutational rate across diverse tumor types and an unusually high r
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Facile, PCR Free Detection of DNA with Single Nucleotide Discrimination

Abstract (Set) Single nucleotide modifications in DNA have important affects on gene expression. This results in significant phenotypic effects. Mutations in genes can also result in the development of diseases, including cancer. The ability to rapidly analyze DNA sequence is useful for correlating mutations with gene expression, and cellular properties. JHU scientists have developed a facile, sensitive method for detecting single nucleotide changes in regions of interest in DNA. Description
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Methods to Reduce Damage from Stroke and Other Neurological Disorders.

Description (Set) Cerebral ischemia (stoke) is an acute neurological condition that is caused by a blood clot that blocks a blood vessel in the brain, while a cerebral hemorrhage (stroke) is caused by a blood vessel that breaks and bleeds into the brain. The result of a stroke is the initiation of an ischemic cascade which includes inflammation but ultimately results in the death of brain cells. Cyclooxygenase (COX)-2 inhibitors are well known for their use as anti-inflammatory agents and have
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Methods to Block or Reduce Damage from Stroke and Other Neurological Disorders

Description (Set) An acute neurological condition caused by blood clots that block blood vessels in the brain is cerebral ischemia (stroke) similarly; blood vessel breakage in the brain is cerebral hemorrhage (stroke). These blood vessel miscues result in the initiation of an ischemic cascade that includes an inflammatory response and ultimately cell death in the brain. Cyclooxygenase (COX)-2 inhibitors are well known for their use as anti-inflammatory agents and have also been shown to reduce
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A Novel Method for Generating and Screening Peptides and Libraries Displayed on Adenovirus Fiber

Abstract (Set) Capsid-displayed adenoviral peptide libraries have been a significant yet unfeasible goal in biotechnology. Three barriers have made this difficult: the large size of the viral genome, the low efficiency of converting plasmid-based genomes into packaged adenovirus, and the fact that adenoviral library amplification is hampered by the ability of two (or more) virus to co-infect one cell. JHU scientists developed a novel method, which is capable of overcoming all three barriers. Fi
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A Novel Cystic Fibrosis Therapeutic Agent

Abstract (Set) JHU scientists found that a known drug has a novel function as a stimulator of calcium-activated Cl- channels (CaCCs) and, thus, is a potential therapeutic drug in the treatment of cystic fibrosis. Cystic fibrosis is the number one genetic killer of children and young adults in the U.S., occurring in about one in 3,000 births. More than 12 million people are carriers of cystic fibrosis. This disease affects the lungs and digestive system of about 30,000 children and adults in the
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Candidate Proteasome Inhibitors that Prevent E6 Mediated p53 Degradation and Selectively Kill HPV+ Cervical Cancer

Abstract (Set) High-risk strains of Human papillomavirus (HPV) cause over 90% of cervical cancers. About 20 million people (men and women) are thought to have an active HPV infection at any given time. Yearly, 14,000 cases are diagnosed and 5,000 women each year die of cervical cancer in the United States. Levels of the tumor suppressor protein p53 were found to be unusually low due to the presence of infection with high-risk or oncogenic strains of HPV. Expression of HPV proteins E6 and E7 pro
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ER alpha M4, ER alpha U4, p16 M4, p16 U5, Hin1 M1, Hin1 U2, RASSF1a M1, RASSF1a U1, RAR beta M1, RAR beta U1, Twist BM2, Twist BU5, APC M3, APC U3, Cylin D2 M1, Cyclin D2 U4, 14-3-3 M2, 14-3-3 U3, HIC

Abstract (Set) Plasmid DNAs incorporating human gene-specific promoter sequences altered by sodium bisulfite treatment. Description (Set) Proposed Use (Set) Their use includes serving as reagents, standards, or controls for assays of gene methylation (e.g. for determining Methylated or unmethylated gene copy number or for calibration of assay parameters or equipment).
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Novel Immunotherapy for Contact Dermatitis

Abstract (Set) Allergic contact dermatitis, the third most common reason for outpatient dermatology visits, is an immunological disease that gives significant influences on patient's quality of life and makes enormous economical impacts. Current therapies for contact dermatitis are largely dependent on general immunosuppressive drugs, which induce long-term destruction of immune system rendering patients immune-compromised. JHU scientists have identified a specific molecular pathway which plays
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Novel Strategy to Induce Erythropoietin Production in vivo

Description (Set) Anemia is the most common disorder of the blood. For chronic anemia such as induced by renal failure and chemotherapy, administration of recombinant erythropoietin (EPO) protein exhibits powerful therapeutic effects. Unfortunately, this therapeutic efficacy is accompanied by side-effects which include hypertension and thrombosis. JHU scientists identified a molecular pathway which induces potent erythropoietin (EPO) production in vivo, and demonstrated that stimulation of this
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Mouse Model of Human Head and Neck Squamous Cell Carcinoma

Abstract (Set) Cancers of the head and neck, the majority of which arise in the squamous cells that line the mucosal surfaces in the head and neck, account for 3% of all malignancies in the United States. Human head and neck squamous cell carcinoma is a clinically aggressive tumor, and fewer than 50% of head and neck cancer patients survive beyond 5 years. Mouse models make essential contributions to our understanding of the cause, prevention and treatment of cancer. This invention provides a m
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PSMA-targeted Nanoparticles for Therapy of Prostate Cancer

Description (Set) Targeted therapy for cancer has gained considerable importance recently with various improvements not only in target identification, but also in small molecule or antibody development. It has also been demonstrated that polymeric nanoparticles can passively target tumors via the enhanced permeability and retention (EPR) effect. The EPR is a property that considers that some molecules of a certain size tend to accumulate in tumors tissues more than they would accumulate in non-
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Topical Administration of Peripherally Acting Drugs for the Treatment of Chronic Pain

Abstract (Set) Clinicians currently have few therapeutic options when it comes to treating chronic neuropathic pain. Drugs available on the market today are not effective in all patients, and often result in partial alleviation of pain. Multiple studies have shown that opioids are effective in treating chronic pain but their use is limited due to dependancy and side effects of constipation, sedation, and respiratory depression. JHU scientists have discovered a method for providing pain relief w
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The Immediate Early Gene Arc Interacts with Endocytic Machinery and Regulates the Trafficking and Function of Presenilin

Abstract (Set) These discoveries promise a novel approach to develop agents to regulate presenilin 1 (PS 1) and treat Alzheimer's Disease and age-dependent memory decline. PS 1 functions as part of the gamma secretase (protease) complex that acts on amyloid precursor protein (APP), Notch and several other transmembrane proteins. Familial Alzheimer's disease results from mutations of PS 1. Recent studies indicate that gamma secretase activity is blocked by agents that interrupt endocytosis. This
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A "Molecular Switch" in Group 1 mGluR Signaling Relevant to Neurological and Psychiatric Diseases

Abstract (Set) Reported here is the mechanism by which group 1 metabotropic glutamate receptors (mGluR) are co-functional with dopamine receptors or growth factor receptors. This convergent signaling is central to understanding several diseases including drug addiction, inflammatory pain, schizophrenia and movement disorders. Several lines of evidence indicate that mGluR signaling is regulated by the balance between Homer (an adaptor protein) and a drugable enzyme. The reported discoveries targ
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Use of Transcranial Magnetic Stimulation (TMS) to Treat Cerebral Palsy.

Abstract (Set) Prostate-specific membrane antigen (PSMA) is a cell surface protein that is over expressed in prostate cancer, including hormone-refractory and metastatic disease. Successful imaging of xenografts that express the prostate-specific membrane antigen (PSMA) using small animal PET and PSMA inhibitors has led to the investigation of the possibility of utilizing the more economical radionuclide 99mTc for SPECT imaging of prostate cancer. JHU scientist have prepared novel 99mTc/Re chel
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Radiolabeled Inhibitors for Prostate Cancer Imaging & Therapeutics

Abstract (Set) Prostate-specific membrane antigen (PSMA) is a cell surface protein that is over expressed in prostate cancer, including hormone-refractory and metastatic disease. Successful imaging of xenografts that express the prostate-specific membrane antigen (PSMA) using small animal PET and PSMA inhibitors has led to the investigation of the possibility of utilizing the more economical radionuclide 99mTc for SPECT imaging of prostate cancer. JHU scientist have prepared novel 99mTc/Re chel
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Functional Isolation of the Low-oxygenic Niche Derived Primitive Hematopoietic Stem Cells By Differential ROS Activity

Abstract (Set) A low-oxygenic niche in bone marrow limits reactive oxygen species (ROS) production, thus providing long-term protection for hematopoietic stem cells (HSCs) from ROS stress. Although many approaches have been used to enrich HSCs, none have been designed to isolate primitive HSCs located within the low-oxygenic osteoblastic-niche due to difficulties of direct physical access. Scientists at JHU show that an early HSC population that might reside in the osteoblastic niche can be fun
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Diagnosis, Prevention and Treatment of Eye Injury and Infection

Abstract (Set) According to the World Health Organization, cataracts are the leading cause of blindness in the world. Approximately 10 million cataract operations are performed each year in the world. By the year 2020 the number of blind people over 60 years of age will likely reach 54 million world-wide if current trends continue. Although it is usually treated successfully, there are a number of surgery-related complications that are of considerable clinical importance. Currently, there is no
...

PET32a O-GlcNAcase-thioredoxin Expression Vector

Abstract (Set) Expression and Purification of Recombinant and Endgenous 0- GlcNAcase The human O-GlcNAcase cDNA was subcloned into the commercial expression bacterial expression vector PET32a (Novagen, Madison, WI), as was the shorter splice variant (MGEA5 s), to yield fusion proteins with thioredoxi. Both construct were confirmed by sequencing. Description (Set) Proposed Use (Set) This expression vector is used to express active O-GIcNAcase in E. coli. It produces a fusion protein betwee
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Protein Expression Markers for the Detection of Thyroid Cancer Metastasis

Description (Set) Approximately 80% of all thyroid cancers are papillary thyroid carcinomas (PTCs). Correct staging of PTC is one of the most important parts of the work up of patients for both prediction of outcome and determination of the most appropriate treatment. PTC generally has a high cure rate with standard surgical and adjuvant radioiodine treatment of neck lymph node metastases (LNM) which are found in 30-65% of initial diagnoses of PTCs. Fifteen percent of cases with LNMs also displ
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Regional Chemotherapy with 4-Hydroperoxycyclophosphamide (Intrathecal Administration)

A method of treating human tumors is provided in which tumors are regionally treated with a cytolytic, pre-activated, bifunctional alkylating agent, namely 4-hydroperoxycyclophosphamide. The treatment is effective against a variety of tumor types within the central nervous systems. Cyclophosphamide, an alkylating agent with a broad spectrum of anti-tumor activity is actually a prodrug which, to be activated must be converted by hepatic microsomal enzymes into 4-hydrocyclophosphamide. This compou
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Method of Retarding the Progression of Chronic Renal Failure

Progression of chronic renal failure can be retarded (slowed or arrested) by administering to humans suffering from such disorder an agent which suppresses the production of glucocorticoids in the human. The agents may be administered alone or in combination with a protein restricted and/or phosphorus restricted diet. Examples of suitable agents which either suppress production of glucocorticoids or block binding to their receptors include sodium valproate, enkephalins, opioids, clonidine, ketoc
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BRCA2 KO Lines for Drug Discovery

BRACA2 is a DNA repair gene. Mutations in BRACA2 cause an inherited risk of breast, ovarian and pancreatic cancers. The cancer cell lines are engineered to lack BRACA2 and are useful screening tools for chemotherapeutic or chemopreventative agents Description (Set) Proposed Use (Set) The BRACA2 KO cell line is a useful screening tools for chemotherapeutic or chemopreventative agents.
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Novel Drug Development for Cancer

vivo. Although, the BPU analogs are effective in the palliative management of a wide variety of human malignancies, their utility as chemotherapeutic agents is limited for several reasons: 1) They are almost insoluble in water; 2) they are difficult to formulate; and 3) they have relatively low bioavailability. JHU scientists have addressed these shortcomings by synthesizing and evaluating sulfur and oxygen analogs of these BPU derivatives. The present invention relates to design and synthesis o
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CanScript for Gene Therapy: a DNA Enhancer Element Conveying Cancer-specific Expression to 30% of Human Malignancies

One of the major requirements for effective cancer gene therapies are DNA sequences that, when inserted into a gene-expression construct, will drive high-level gene expression exclusively in cancer cells. These sequences are termed cancer specific enhancers or promoters of gene transcription. Until now, no highly specific enhancer has been identified that can drive high levels of gene expression in greater than 10% of human malignancies. An invention by JHU scientists now describes a short DNA
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Novel Protein Increases Muscle Mass

Muscle mass is normally regulated by the activities of members of the transforming growth factor-B (TGF-B) superfamily, which act to limit muscle growth. Scientists at JHU have now found a protein that is capable of binding certain members of the TGF-B family and inhibiting their activities in vitro. Therefore this protein has important applications for treating human muscle degenerative diseases as well as for enhancing livestock production. Description (Set) Proposed Use (Set) There are
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Specific Absorption Rate (SAR) Dosimeter for RF Power Deposition in MRI

The present invention addresses the critical need for direct and independent SAR by providing the first dosimeter for MRI which directly measures standard average head and body SAR. The device uses a transducer placed in the scanner, comprised of induction looping a resistive load and a small MRI set-up sample. The voltages induced in the loops are measured and calibrated to provide direct measures of the average SAR, independent of the scanner Devices that are tested to be safe at a specified s
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An Assay to Identify Novel Targets and Personalize Combination Anticancer Therapy

Scientists at JHU have developed a pharmacodiagnostic assay to identify relevant targets and direct the design of individual patient combination anticancer therapy. The assay is based on fine needle aspiration of a neoplastic lesion, exposing the acquired materials ex vivo to control media or media with the drug corresponding to the standard of care for the subject's disease, and processing of the material for comparative mRNA analysis. The assay allows one to rationally seek for relevant target
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Serum Response Factor (SRF) Conditional Knockout Mice

Scientists at JHU have created conditional serum response factor (srf) knockout mice to investigate the plasticity of adult neurons. The mice were generated to circumvent the embryonic lethality associated with Srf null mice and allow for the ablation of Srf in a spatially- and temporally-restricted manner.
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Myc-repressed microRNAs as Novel Anti-cancer Therapeutic Agents

MicroRNAs (miRNAs) are 18-24 nucleotide RNA molecules that regulate the stability or translational efficiency of target mRNAs. miRNAs are frequently dysregulated in cancer cells and can act as oncogenes and tumor-suppressors. The proto-oncogene c-MYC encodes a transcription factor that regulates cell proliferation, growth, and apoptosis. Dysregulated expression or function of c-Myc is one of the most common abnormalities in human malignancy. JHU and University of Pennsylvania scientists have ide
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A Novel Agent to Reduce the Requirement of Analgesics in Clinical Pain Management

N-methyl-d-aspartate (NMDA) receptor activation has been demonstrated to play an important role in the processing of spinal nociceptive information. Postsynaptic density protein-95 (PSD-95), a scaffolding protein, has been identified to attach NMDA receptors to internal signaling molecules at neuronal synapses. Previous studies have shown that the expression of spinal PSD-95 is critical for NMDA receptor-mediated pain hypersensitivity, and that spinal PSD-95 knockdown inhibits the development an
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293 Cell Line for Flag-tagged HIMF (flag-HIMF-293)

Hypoxia-induced mitogenic factor (HIMF) is highly up regulated in the lungs as a result of hypoxia-induced pulmonary hypertension, and has proangiogenic and proinflammatory effects in the lung. As a research tool for studying the function and activity of HIMF, JHU researchers have developed a transgenic HEK 293 cell line expresses a Flag-tagged HIMF. Expression of the Flag-tagged HIMF protein can be induced by tetracycline and most of the protein is secreted into the culture medium where it can
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Mutations of the Cholecystokinin Receptor Type 2 in Human Cancers

Gastrin and the cholecystokinin type B/gastrin receptor (CCKBR) have been shown to be expressed in colorectal adenocarcinoma. Colorectal adenocarcinoma is the second leading cause of cancer mortality in the United States. The CCKBR was screened for mutations in colon, lung, and breast, gastric, pancreatic, ovarian, medullobastoma, and glioblastoma tumors. Somatic mutations were found at frequencies above background somatic mutation rates suggestive of functional significance. This invention inc
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Quantitative Detection of Promoter Hypermethylation as a Biomarker of Acute Kidney Injury During Transplantation

Kidney repair after injury is a recapitulation of normal morphogenesis, with similarities to malignant transformation, and aberrant promoter hypermethylation, also known as epigenetics, is a promising biomarker approach to diagnose malignancies. Therefore, JHU researchers investigated whether methylation-based urinary biomarkers could be useful for the early detection of ischemic-reperfusion injury (IRI). Examination of urine DNA for aberrant promoter hypermethylation pattern in two cancer-relat
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Formulation of Toll-like Receptor Ligand with Whole Cell Tumor Vaccine to Enhance Anti-tumor Responses

Established reports in literature have shown that the innate immune system can modulate immune responses against cancer. Lipopolysaccharide (LPS), a Toll-like receptor ligand 4 (TLR4), was absorbed onto whole cell tumor vaccine using a liposome formulation. The liposome reagent was titrated with LPS for greater than 95% complex formulation onto whole tumor cells. The LPS absorbed whole tumor cells had been previously modified to secrete genetically modified cerebrospinal fluid (GM-CSF). After ab
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A Functional Assay Distinguishing Pathogenic and Nonpathogenic Variant Alleles of the BRCA2 Gene

Some inherited mutations that inactivate BRCA2 and cause a high familial risk of breast, ovarian, and pancreatic cancer are common in the population. Other mutations are less frequent and referred to as Variants of Unknown Clinical Significance (VUCS). These individual gene mutations are uncharacterized with regard to their effect on gene function, the risk of cancer development and penetrance of the cancer phenotype. As a result, clinicians have difficulty in providing the patient the appropria
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Toxic Role of Heme Oxygenase in Hemorrhage

JHU scientists have investigated the contribution of inducible HO-1 to early brain injury produced by intracerebral hemorrhage (ICH). In vivo studies determined that after induction of ICH, HO-1 proteins are highly detectable in the peri-ICH region predominantly in microglia/macrophages and endothelial cells. Injury volume was found to be significantly smaller in HO-1 knockout (HO-1-/-) mice than in wildtype controls 24 and 72 h after ICH. The protection in HO-1-/-mice was associated with a mar
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Development of a Combinational Paradigm Combating HIV, HIV/HSV or HIV/HPV Infections in Humans

million have been infected in the year 2006 alone. AIDS death reached 2.9 million this year which is the highest ever reported in any year. There are 40,000 new AIDS cases a year in U.S. along. The anti-HIV compounds that are currently being used to treat AIDS patients include several reverse transcriptase inhibitors. However, these drugs gradually lose their effectiveness with the appearance of a group of populated viral mutants. Thus, development of alternative therapeutic methods that inhibit
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Biofunctional Fibers

The present invention is directed to surface functionalization of polymeric fibers. Surface biofunctionalization is achieved by covalent conjugation of biofunctional igands and/or cell growth factors that are crucial for cell attachment, proliferation and functions. Biofunctional fibers could be fabricated into three-dimensional scaffolds. Polymer fibers described here comprise of biocompatible polymers that are either biodegradable ornon-biodegradable. This patent also describes a series of new
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Methods for Inducing Immune Tolerance By Inhibiting the Fas Pathway

Fas ligand ("FasL") is a protein with an activity to induce apoptosis of a Fas antigen ("Fas")-expressing cell. Apoptosis of the Fas antigen-expressing cells is believed to be induced by binding of FasL with Fas on the cell surface, which results in the transfer of an apoptosis signal to the cell via the Fas antigen. Natural loss-of-function mutations of FasL (gld) and Fas (lpr) that inhibit the function of Fas pathway prevent T cell-mediated autoimmune diseases. However, since inhibition of the
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Derivation of Multipotent Mesenchymal Cells from Human Embryonic Stem Cells and Biomaterials-Directed in Vivo Commitment

Heterogeneous differentiation of human embryonic stem cells (hESC) limits the potential use of hESCs for cell-based therapies and studies of specific differentiation programs. Clearly, more efficient and selective methods are needed to direct the proliferation and differentiation of hES cells to produce a homogeneous population of specific cell types for regeneration applications. In this report, we describe an efficient derivation and characterization of mesenchymal-precursor cells having multi
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Persistence Switch Involved in Persister Formation and Tolerance to Multiple Antibiotics and Stresses, as a Drug Target for Persister Bacteria

Persister bacteria are metabolically quiescent cells that neither grow nor die in the presence of antibiotics. The biological mechanism of bacterial persistence is not fully understood but it is clear that they reduce antibiotic efficacy, which leads to millions of infectious disease deaths each year. Recently, researchers at The Johns Hopkins University discovered a gene that appears to be involved in persistence formation in E. coli. An E. coli mutant for the persister gene demonstrated high s
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Controlled Microencapsulation of Mammalian Cells

The present invention is directed to an automated device and method to prepare polyelectrolyte microcapsules for controlled microencapsulation of mammalian cells. This device and method delivers precision control of volume of microcapsules at picoliter scale with narrow size distribution and with controlled thickness of the capsule membrane. Microcapsules generated in this invention are useful for culturing mammaliam cells. Particularly, the present invention describes a method for microencapsul
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MKK4/MAP2K4 KO Lines for Drug Discovery

Description (Set) MKK4 (JNKK1, MAP2K4, and SEK1), a dual-specificity kinase gene on chromosome 17p11, is a tumor suppressor gene that is mutated in a subset of most tumor types. MKK4 protein is activated by several kinases, and active MKK4 cooperates with MKK7 to phosphorylate and thereby activate Jun NH2-terminal kinase (JNK) in the stress-activated cascade. This tumor suppressor gene is distinguished by a remarkably consistent mutational rate across diverse tumor types and an unusually high r
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Detection of Antibodies to Specific Glycans as a Novel Diagnostic Test for Autoimmune Disease

Abstract (Set) JHU scientists have identified biomarkers specific for autoimmune disease. Systemic autoimmune diseases are life- threatening, complex and can involve various organ systems including skin, joints, the renal system, the hematopoietic system, and the cardiovascular system. In autoimmune disorders, antibodies target self-molecules known as autoantigens. The identification of universal and specific autoantigens as diagnostic tools for disorders such as lupus or scleroderma is of grea
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Facile, PCR Free Detection of DNA with Single Nucleotide Discrimination

Abstract (Set) Single nucleotide modifications in DNA have important affects on gene expression. This results in significant phenotypic effects. Mutations in genes can also result in the development of diseases, including cancer. The ability to rapidly analyze DNA sequence is useful for correlating mutations with gene expression, and cellular properties. JHU scientists have developed a facile, sensitive method for detecting single nucleotide changes in regions of interest in DNA. Description
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Methods to Block or Reduce Damage from Stroke and Other Neurological Disorders

Description (Set) An acute neurological condition caused by blood clots that block blood vessels in the brain is cerebral ischemia (stroke) similarly; blood vessel breakage in the brain is cerebral hemorrhage (stroke). These blood vessel miscues result in the initiation of an ischemic cascade that includes an inflammatory response and ultimately cell death in the brain. Cyclooxygenase (COX)-2 inhibitors are well known for their use as anti-inflammatory agents and have also been shown to reduce
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A Novel Method for Generating and Screening Peptides and Libraries Displayed on Adenovirus Fiber

Abstract (Set) Capsid-displayed adenoviral peptide libraries have been a significant yet unfeasible goal in biotechnology. Three barriers have made this difficult: the large size of the viral genome, the low efficiency of converting plasmid-based genomes into packaged adenovirus, and the fact that adenoviral library amplification is hampered by the ability of two (or more) virus to co-infect one cell. JHU scientists developed a novel method, which is capable of overcoming all three barriers. Fi
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A Novel Cystic Fibrosis Therapeutic Agent

Abstract (Set) JHU scientists found that a known drug has a novel function as a stimulator of calcium-activated Cl- channels (CaCCs) and, thus, is a potential therapeutic drug in the treatment of cystic fibrosis. Cystic fibrosis is the number one genetic killer of children and young adults in the U.S., occurring in about one in 3,000 births. More than 12 million people are carriers of cystic fibrosis. This disease affects the lungs and digestive system of about 30,000 children and adults in the
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MicroRNA Expression and Control of Stem Cell Pluripotency, Differentiation and Cellular Processes

MicroRNAs (miRNAs) are a class of short non-coding RNAs that bind to the 3' untranslated region of mRNAs and downregulate their translation to protein. MiRNAs play critical roles in many different cellular processes including metabolism, apoptosis, differentiation, and development. Researchers at JHU have investigated the role of miRNAs in the control of stem cell biology and differentiation. Through this work they discovered 33 novel miRNAs expressed by both bone marrow or mobilized peripheral
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Reversal of Aberrant Cancer Gene Silencing By Inhibition of SIRT1

The class III histone deactylase (HDAC), SIRT1, has cancer relevance because it regulates lifespan in multiple organisms, down-regulates p53 function through deacetylation, and is linked to polycomb gene silencing in Drosophila. JHU inventors have shown that SIRT1 localizes to promoters of several aberrantly silenced tumor suppressor genes (TSGs) in which CpG islands are densely hypermethylated, but not to these same promoters in cell lines in which the promoters are not hypermethylated and the
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Generation of Xenografts on Mice to Tailor Anticancer Therapy from Fine Needle Aspiration Biopsies

Xenografts can be made from resected tumors of pancreatic cancer patients in order to generate tumor tissue for the testing of tailored drug regimes. However, the majority of patients with pancreatic cancer (and other types of cancer) are not candidates for tumor resection. Scientists at Johns Hopkins University have developed a method for generating tumors xenografted on nude mice using another technique, fine needle aspiration (FNA) biopsy. FNA biopsy is preferable to the traditional method of
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Inhibitors of Human Methionine Aminopeptidase 1 As Novel Anticancer Drug Leads

JHU researchers have unraveled an important function of human type 1 aminopeptidase (hMetAP1) in the timely progression of cells through mitosis. In addition, a new structural class of isoform specific inhibitors of hMetAP1 has been discovered that induces a significant delay in mitosis (>3 hrs) and further, to cause death in leukemia cell lines. This discovery suggests that inhibition of hMetAP1 alone may be sufficient to inhibit tumor growth. Description (Set) Proposed Use (Set) Approxima
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Development of a Biomarker for the Early Detection of Diabetes ("Pre-Diabetes") Based Upon O-GlcNAc

JHU scientists have identified markers in human blood that might readily identify individuals at the early stages of type II diabetes, when life-style changes will be most effective in disease prevention. The markers derive from hexosamine metabolism, and in particular the dynamic O-GlcNAc (modification of proteins by the sugar N-acetyl glucosamine) of proteins that causes insulin-resistance and is responsible for glucose toxicity. It appears that several O-GlcNAc modified proteins and a specifi
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Controlled Local Delivery of Glucosamine (GlcN) for Musculoskeletal Repair

The novel feature of this discovery is the application of GlcN to joint and spine (cartilage) tissue engineering/repair. Because of this discovery of the relevant concentrations one can envision new therapies such as: 1.) injection into the joint space of GlcN in drug delivery vehicles after microfracture 2.) implantation of a scaffold releasing GlcN at the appropriate rate into a defect space 3.) incorpation of GlcN into a tissue glue to enhance repair
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Pkd1 T3041R: Pkd1 Cleavage-deficient Mouse

Scientists at JHU have created a novel mouse model of polycystic kidney disease (PKD), one of the most common human genetic disorders. The mice are deficient in Pdk1 cleavage due to missense mutations with the polycystin-1 gene. The mice develop polycystic disease during neo-natal development but unlike previous models of PKD, the mice survive until 3 months of age. Description (Set) Proposed Use (Set) PKD is one of the most common hereditary diseases in the US, affecting more than 500,000
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An Approach to Guide the Individualize Development of New Anticancer Agents

The development of new oncology drugs has not resulted in a meaningful improvement in survival of cancer patients. Major reasons are the lack of criteria, models and guidelines to develop individualized treatment plans most likely to produce a successful clinical outcome in a given patient. JHU scientists have developed and optimized a new approach to address this problem. The inventive process involves the generation of live xenograft mouse models using resected tumor tissue obtained during sur
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Use of EP3 Receptor Ligands to Reduce Damage from Stroke and Other Neurological Disorders

Cyclooxygenase (COX)-2 inhibitors are well known for their use as anti-inflammatory agents and have also been shown to reduce the damage associated with neuroinflammatory disorders. They act by inhibiting the production of prostaglandins, many of which mediate the inflammatory response. The effect of PGE (2) EP3 receptors on injury size was investigated following cerebral ischemia and induced excitotoxicity in mice. JHU scientists have found that stimulating the EP3 receptor with an agonist incr
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Use of Novel Derivatives of Artemisinin to Inhibit Toxoplasma Gondii

Toxoplasmosis, caused by the protozoan Toxoplasma gondii, is a medically important infection that is distributed worldwide. Current treatments for the prevention and treatment of toxoplasmosis are of limited efficacy and have undesirable side-effects. According to recent studies, the natural product artemisinin and its derivatives are excellent alternatives and are very effective at inhibiting toxoplasma replication in vitro. Although artemisinin has additional therapeutic qualities such as rapi
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Inhibition of Transplantation Rejection By Suppressing HVEM

Decoy lymphotoxin beta receptor (LT? has potent immune inhibitory activities, and thus represents a promising biologic for the treatment of inflammation, autoimmune diseases and graft-versus-host disease (GVHD). As this reagent interrupts multiple molecular interactions, including LT?T?and LIGHT-HVEM/LT? underlying molecular mechanisms have yet to be fully understood. Scientists at JHU have now determined that blockade of the LIGHT-HVEM pathway is sufficient to induce amelioration of GVHD in mou
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Histone Mutant Plasmid Collections

Epigenetics, the study of stable alterations in gene expression that arise during development and cell proliferation, is a popular area of modern bioscience. The epigenetic context in which genes are expressed is important for understanding normal phenotypic variation and disease. DNA methylation has been the most comprehensively studied epigenetic mark to date, but numerous studies involving histone modification and chromatin structure are now being conducted. Recently, scientists at the Johns
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Use of High Dose Cyclophosphamide in Combination with Anti-Idiotypic Vaccines in Anti-cancer Therapy

Recently, immunotherapy based techniques have been developed for the treatment of various cancers. The central premise underlying immunotherapy for cancer is the presence of antigens which are selectively or abundantly expressed or mutated in cancer cells. Despite advances in the development of various immune therapies for treatment of cancer, immune tolerance presents a major barrier. Therefore, there is a need to identify therapies or agents which may be used to break immune tolerance which s
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Use of High Dose Cyclophosphamide in Combination with Immune Therapeutics in Anti-Cancer Therapy

Cyclophosphamide and especially high-dose cyclophosphamide has been used for the treatment of certain autoimmune diseases such as severe aplastic anemia. Low to intermediate doses of cyclophosphamide have also been used in combination chemotherapy for treating certain cancers and appears to work either by stopping the growth of cancer cells or by killing the cancer cells. However, remaining cancer cells, especially cancer stem cells, are able to divide and lead to relapse. More recently, immune-
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Method of Identifying Patients Suitable for High-Dose Cyclophosphamide Treatment

Aldehyde dehydrogenases (ALDHs) are intracellular enzymes responsible for oxidizing aldehydes. A number of studies seem to suggest that ALDH confers resistance of cells to cyclophosphamide, which is routinely used for the treatment of autoimmune diseases and cancer. High dose cyclophosphamide therapy appears to be more effective than the low-dose therapy, although high dose cyclophosphamide therapy is not suitable for all patients because of higher toxicity. It is generally difficult to predict
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Rapid Predictive Assay for Malignancies Treated with Polo-like Kinase-1 (PLK1) Inhibitors and Cell Cycle Modulators

Scientists at JHU have developed a pharmacodiagnostic assay for patients treated with targeted therapies directed at modulating the cell cycle, including but not limited to Polo-Like Kinase-1 (PLK1) inhibitors. The assay uses a fine needle aspirate (FNA) of a neoplastic lesion that is exposed ex vivo to a candidate drug therapy. Messenger RNA (mRNA) expression analysis can then determine the sensitivity of the lesion to treatment. As a result, the most effective course of treatment can be determ
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Protective Effect of Carbon Monoxide (CO) in Stroke

Carbon monoxide (CO) has been shown to mediate both beneficial and deleterious processes in the central nervous system depending on its concentration. CO is produced endogenously through the breakdown of hemoglobin by heme oxygenase (HO) to produce biliverdin, iron and CO, and these physiological concentrations of CO play an important role in many protective mechanisms. Low levels of exogenous CO can also bring about anti-inflammatory and anti-apoptotic effects and mediate vascular signaling tha
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Novel Breast Cancer Cells That Stably Express a New Orange-red Fluorescent Protein Provides Facile Tracking of Metastatic Progression in Live Animal Model Systems

Modeling human disease states in mice has a profound impact on our understanding of disease progression and treatment development. The need for noninvasive longitudinal monitoring of such model systems has resulted in the development of small animal imaging technologies. Endogenously expressed fluorescence protein?based optical imaging has the advantage of not requiring administration of an enzymatic substrate, targeted fluorescent or radionuclide reporter, nor a contrast agent. JHU researchers
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Small Molecules Targeting 5-meCpG Binding Domain Proteins (MBDs) for Cancer Prevention and Treatment, and for Other Clinical Uses

JHU scientists have discovered new small molecules inhibitors of the transcriptional repression pathway mediated by the 5m-CpG-binding family domain proteins (MBD), MBD2 and MeCP2. Both MBD2 and MeCP2 have been found to play critical roles in both the epigenetic silencing of gene in human cancer cells and in the development of intestinal adenomas in APC min/+ mice. The inhibitors describes in this invention can be used to reverse the epigenetic silencing of critical genes that accompanies cancer
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Inhibition of Vif-Cul5 Interaction Through Use of Zinc Chelator

The Vif ? A3G axis is one of the most promising new drug targets in HIV research. Previous studies have discovered that Vif is an HIV-derived protein that acts to derail antiviral efforts from the host cell. Vif tags Apobec3G (A3G), an antiviral host factor, with ubiquitin for destruction in the 26S proteasome in addition to partially inhibiting the expression of new A3G proteins. In the absence of Vif, A3G is incorporated into the HIV virus and severely decreases the infectivity of progeny vir
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Methods to Reduce Damage from Stroke and Other Neurological Disorders.

Description (Set) Cerebral ischemia (stoke) is an acute neurological condition that is caused by a blood clot that blocks a blood vessel in the brain, while a cerebral hemorrhage (stroke) is caused by a blood vessel that breaks and bleeds into the brain. The result of a stroke is the initiation of an ischemic cascade which includes inflammation but ultimately results in the death of brain cells. Cyclooxygenase (COX)-2 inhibitors are well known for their use as anti-inflammatory agents and have
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Low Voltage, High Mass Range Ion Trap Mass Spectrometer for Mars Atmosphere and Other Harsh Environments

The invention is an ion trap mass spectrometer designed for use on the surface of Mars to detect minute qualities of organic and biological compounds that might indicate the presence of life or former life on that planet. Though compact, the instrument has a high mass range that will enable it to focus on the larger, less volatile molecules that would be less likely to have escaped the atmosphere. The technology has the potential for use in a number of other applications in environmental monitor
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Methods to Block or Reduce Damage from Stroke and Other Neurological Disorders

Description (Set) An acute neurological condition caused by blood clots that block blood vessels in the brain is cerebral ischemia (stroke) similarly; blood vessel breakage in the brain is cerebral hemorrhage (stroke). These blood vessel miscues result in the initiation of an ischemic cascade that includes an inflammatory response and ultimately cell death in the brain. Cyclooxygenase (COX)-2 inhibitors are well known for their use as anti-inflammatory agents and have also been shown to reduce
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Tamoxifen-resistant MCF-7 Breast Cancer Cell Line

MCF-7 cells serve as a prototypic model system for human breast cancer and their response to secretive endocrine response modifiers (SERM) therapy. MCF-7 cells, upon long term exposure to tamoxifen, acquire tam-resistance and continue to grow in the presence of tamoxifen. Description (Set) Proposed Use (Set) This invention provides a cell-based model, both in vitro and in vivo, for tam-resistant breast cancer, allows the testing of alternate therapies to tam-resistant breast cancer, or the
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Novel Inhibitors of CRAC Channel As Immunosuppressants

T cell activation is essential for immune response. It is primarily responsible for graft rejection upon organ transplantation. Moreover, dysregulation of T cells has been implicated in several autoimmune disorders, including MS and rheumatoid arthritis. Existing immunosuppressive drugs have significant toxicity, preventing them from being used in a wider range of autoimmune related disorders. Scientists at JHU have now established an IL-2 luciferase reporter cell line that can be activated by a
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A Novel Method for Generating and Screening Peptides and Libraries Displayed on Adenovirus Fiber

Abstract (Set) Capsid-displayed adenoviral peptide libraries have been a significant yet unfeasible goal in biotechnology. Three barriers have made this difficult: the large size of the viral genome, the low efficiency of converting plasmid-based genomes into packaged adenovirus, and the fact that adenoviral library amplification is hampered by the ability of two (or more) virus to co-infect one cell. JHU scientists developed a novel method, which is capable of overcoming all three barriers. Fi
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Antipsychotic Drugs that do not elicit Weight Gain by Screening for Influences on Hypothalamic AMP-Kinase and Histamine H1 Receptors

Atypical antipsychotic drugs (AAPDs) have markedly enhanced the treatment of schizophrenias but their use has been hindered by the major side effect of weight gain. Ignorance of the mechanism of these orexigenic actions has delayed efforts to develop alternative therapeutic agents. JHU scientists have now discovered that orexigenic AAPDs potently and selectively activate hypothalamic AMP kinase (AMPK), an action abolished in mice with deletion of histamine H1 receptors (H1R). These findings may
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A Novel Cystic Fibrosis Therapeutic Agent

Abstract (Set) JHU scientists found that a known drug has a novel function as a stimulator of calcium-activated Cl- channels (CaCCs) and, thus, is a potential therapeutic drug in the treatment of cystic fibrosis. Cystic fibrosis is the number one genetic killer of children and young adults in the U.S., occurring in about one in 3,000 births. More than 12 million people are carriers of cystic fibrosis. This disease affects the lungs and digestive system of about 30,000 children and adults in the
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The Consensus Coding Sequences of Breast and Colorectal Cancers

Human cancer is a disease caused by an accumulation of mutations in genes involved in cell cycle regulation. To date only a small fraction of the genes underlying tumorigenesis have been identified, and the number and type of genes responsible are unknown. With the sequencing of the human genome researchers have the capacity to detect genetic alterations in cancers in unprecedented detail. JHU scientists have undertaken a systematic analysis of well-annotated human protein-coding genes in both b
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Melanoma Metastasis-associated Genes As Novel Diagnostic and Prognostic Markers and Therapeutic Targets for Melanoma

The incidence of melanoma is increasing at the highest rate for any form of cancer in the United States and the current lifetime risk in the US is 1 in 68. Presently there are few effective systemic therapies to treat advanced stages of melanoma and the key to improved survival in all affected individuals remains early diagnosis and treatment. Recently, however, scientists at JHU identified novel candidate genes that are diagnostic for the development of a tumor-associated vasculature and metast
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Genomewide Analysis of Chromosomal Translocation

Abstract (Set) The cancer genome is quite unstable with many aberrant genetic translocations dispersed across its sequence. Many chromosomal translocations result in chimeric transcripts resulting in elevated expression levels, believed to be responsible for tumorigenesis. Therefore, it is important to identify these chimeric transcripts, which can be reliable markers for early detection, and provide new targets for drug development. Currently available methods only allow the identification of
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Monoclonal Antibodies (mAb) Specifically Against Mouse and Human LIGHT and B7-H4

Scientists at JHU have made antibodies against mouse and human LIGHT and B7-H4 molecules. Description (Set) Proposed Use (Set) These mAb could be used specifically to detect the expression of LIGHT and B7-H4 and to block interactions of these molecules with their counter-receptors.
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Candidate Proteasome Inhibitors that Prevent E6 Mediated p53 Degradation and Selectively Kill HPV+ Cervical Cancer

Abstract (Set) High-risk strains of Human papillomavirus (HPV) cause over 90% of cervical cancers. About 20 million people (men and women) are thought to have an active HPV infection at any given time. Yearly, 14,000 cases are diagnosed and 5,000 women each year die of cervical cancer in the United States. Levels of the tumor suppressor protein p53 were found to be unusually low due to the presence of infection with high-risk or oncogenic strains of HPV. Expression of HPV proteins E6 and E7 pro
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Selective Fluorescent Labeling of S-Nitrosothiols: S-FLOS

Johns Hopkins University is seeking licensees for a powerful analytical and quantitative technique for S-nitrosylation of proteins. S-nitrosylation is a post-translational protein modification that can alter protein structure and activity. Emerging evidence suggests that dysregulation of S-nitrosylation is related to the pathophysiology of many disease processes. To date, multiple proteins associated with neurodegenerative disease were found to be S-nitrosylated. Identified S-nitrosylated protei
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ER alpha M4, ER alpha U4, p16 M4, p16 U5, Hin1 M1, Hin1 U2, RASSF1a M1, RASSF1a U1, RAR beta M1, RAR beta U1, Twist BM2, Twist BU5, APC M3, APC U3, Cylin D2 M1, Cyclin D2 U4, 14-3-3 M2, 14-3-3 U3, HIC

Abstract (Set) Plasmid DNAs incorporating human gene-specific promoter sequences altered by sodium bisulfite treatment. Description (Set) Proposed Use (Set) Their use includes serving as reagents, standards, or controls for assays of gene methylation (e.g. for determining Methylated or unmethylated gene copy number or for calibration of assay parameters or equipment).
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New Angiogenesis Inhibitors Derived from Fumagillin

Angiogenesis, the formation of new blood vessels, is involved in the pathogenesis of a number of common human diseases, including cancer, diabetic retinopathy, rheumatoid arthritis, and macular degeneration. Inhibitors of angiogenesis can be used for the treatment of all aforementioned disease conditions as well as other angiogenesis-dependant illnesses. The fumagillin family of natural products have previously been reported to be potent inhibitors of angiogenesis. Researchers at JHU have discov
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Novel Immunotherapy for Contact Dermatitis

Abstract (Set) Allergic contact dermatitis, the third most common reason for outpatient dermatology visits, is an immunological disease that gives significant influences on patient's quality of life and makes enormous economical impacts. Current therapies for contact dermatitis are largely dependent on general immunosuppressive drugs, which induce long-term destruction of immune system rendering patients immune-compromised. JHU scientists have identified a specific molecular pathway which plays
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Mouse Model of Human Head and Neck Squamous Cell Carcinoma

Abstract (Set) Cancers of the head and neck, the majority of which arise in the squamous cells that line the mucosal surfaces in the head and neck, account for 3% of all malignancies in the United States. Human head and neck squamous cell carcinoma is a clinically aggressive tumor, and fewer than 50% of head and neck cancer patients survive beyond 5 years. Mouse models make essential contributions to our understanding of the cause, prevention and treatment of cancer. This invention provides a m
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PSMA-targeted Nanoparticles for Therapy of Prostate Cancer

Description (Set) Targeted therapy for cancer has gained considerable importance recently with various improvements not only in target identification, but also in small molecule or antibody development. It has also been demonstrated that polymeric nanoparticles can passively target tumors via the enhanced permeability and retention (EPR) effect. The EPR is a property that considers that some molecules of a certain size tend to accumulate in tumors tissues more than they would accumulate in non-
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MicroRNAs As Targets for Anti-Angiogenic Therapies

MicroRNAs (miRNAs) are 18-24 nucleotide RNA molecules that regulate the stability or translational efficiency of target mRNAs. miRNAs are frequently dysregulated in cancer cells and can act as oncogenes and tumor-suppressors. The proto-oncogene c-MYC encodes a transcription factor that regulates cell proliferation, growth, and apoptosis. Dysregulated expression or function of c-Myc is one of the most common abnormalities in human malignancy. The MYC cancer gene increases the expression of a clu
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Drugs Targeting Cancer Stem Cells

The study of cancer stem cells is important for the development of new cancer treatments. Existing chemotherapeutics may eradicate the bulk of a tumor but residual stem cells may remain to initiate new tumor growth. Novel treatments that target cancer stem cell will help to eliminate the source of the cancer and increase remission times. Using a compound library screening strategy, researchers at JHU have identified a compound, a metal chelator and NF-?inhibitor, which exhibits preferential act
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Topical Administration of Peripherally Acting Drugs for the Treatment of Chronic Pain

Abstract (Set) Clinicians currently have few therapeutic options when it comes to treating chronic neuropathic pain. Drugs available on the market today are not effective in all patients, and often result in partial alleviation of pain. Multiple studies have shown that opioids are effective in treating chronic pain but their use is limited due to dependancy and side effects of constipation, sedation, and respiratory depression. JHU scientists have discovered a method for providing pain relief w
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N171-82Q Huntington's Disease (HD) Mouse Model

This transgenic line expresses an N-terminally truncated human huntingtin cDNA that encodes 82 glutamines and encompasses the first 171 amino acids. The altered huntingtin cDNA is under the control of a mouse prion protein promoter. Expression is observed in neurons of the central nervous system. Description (Set) Proposed Use (Set) This mouse model provides an important research tool for studying the pathogenesis of Huntington?s disease.
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Biocompatible "smart" nanogels as carriers for hydrophobic drugs

The present invention involves a biocompatible nanogel composition for the delivery of hydrophobic substances that are not readily soluble in organic solvents. These nanogels have been designed to contain hydrophobic cores which encapsulate the insoluble molecules, and a hydrophilic shell which allows long term systemic circulation. The nanogels constitute discrete particles that are less than 100 nm in size. The compositions are non-toxic and non-antigenic, and are suitable for systemic adminis
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The Immediate Early Gene Arc Interacts with Endocytic Machinery and Regulates the Trafficking and Function of Presenilin

Abstract (Set) These discoveries promise a novel approach to develop agents to regulate presenilin 1 (PS 1) and treat Alzheimer's Disease and age-dependent memory decline. PS 1 functions as part of the gamma secretase (protease) complex that acts on amyloid precursor protein (APP), Notch and several other transmembrane proteins. Familial Alzheimer's disease results from mutations of PS 1. Recent studies indicate that gamma secretase activity is blocked by agents that interrupt endocytosis. This
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Ligands for Nicotinice Acetylcholine Receptors, and Methods of Making and Using Them

One aspect of the present invention relates to heterocyclic compounds that are ligands for nicotinic acetylcholine receptors. A second aspect of the invention relates to the use of a compound of the invention for modulation of a mammalian nicotinic acetylcholine receptor. The present invention also relates to the use of a compound of the invention for treating a mammal suffering from Alzheimer's disease, Parkinson's disease, dyskinesias, Tourette's syndrome, schizophrenia, attention deficit diso
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A "Molecular Switch" in Group 1 mGluR Signaling Relevant to Neurological and Psychiatric Diseases

Abstract (Set) Reported here is the mechanism by which group 1 metabotropic glutamate receptors (mGluR) are co-functional with dopamine receptors or growth factor receptors. This convergent signaling is central to understanding several diseases including drug addiction, inflammatory pain, schizophrenia and movement disorders. Several lines of evidence indicate that mGluR signaling is regulated by the balance between Homer (an adaptor protein) and a drugable enzyme. The reported discoveries targ
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Multitypic Recombinant Adenovirus Vaccines Against HPV

This invention is a vaccine against human papillomavirus infection consisting of recombinant adenoviruses that are engineered to include peptides of the human papillomavirus 16 minor capsid protein (L2). The L2 sequences are displayed on the outside of the adenovirus particles, where they induce immunity directed against HPV16 and, since HPVL2 induces multitypic antibody, against other HPV types as well. The preferred vaccines will be viable and will induce immunity as they replicate in vaccinat
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Small molecule as Novel Anti-tumor and Anti-angiogenic Agent

Abstract (Set) JHU scientists found that a known antibiotic that inhibits the type 2 methionine aminopeptidase and possesses anti-angiogenic activity both in vitro and in vivo. It causes both primary human endothelial cells and cancer cells to undergo senescence. It was demonstrated that this antibiotic is capable of inhibiting tumor growth in vivo in animal models and tumor induced angiogenesis in vivo. Description (Set) Dual-action drug for the treatment of different cancer including breast
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The Direction of Controlled Heat Specifically to Cancer Cells to Enhance Therapeutic Responses to Combination Therapy

The novel featues outlined in ths invention are: 1. The use of localized limited heat that is specifically directed to the cancer cells and their environment to sensitize the cell to combination therapeutic approaches including cytotoxic, immunologic and radiation therapy. 2. To enhance this effect by modulating proteins involved in both nuclear strctue as well as heat shock proteins and molecules to increase heat sensitivity.
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Liposomase for the Tumor-specific Delivery of Chemotherapeutic Drugs

The challenge in developing effective cancer treatments is in achieving specificity so that only cancer cells are destroyed. JHU scientists have developed a novel drug delivery system that overcomes the problems caused by the low specificity of chemotherapeutic drugs. The technology utilizes a unique enzyme, called liposomase, cloned from Clostridia novyi-NT. This enzyme can be delivered to tumors through various means, including attachment to antibodies, incorporation into gene therapy vectors
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Method of Synthesis of Chemospheres and Chemogel, Hydrogel Embolic Particles and Gel for Locoregional Delivery of Therapeutic Agents and Immunoisolation of Cellular Therapeutics

Description (Set) JHU scientists have developed a method of producing innovative biocompatible compositions useful for variety of therapeutic applications. The biocompatible polymer gel compositions are innovative in the way that they incorporate trackable contrast agents and bioactive agents for slow release delivery in the area of interest. In addition, the compositions can be polymerized without the use of heat, which expands their potential applications. Incorporation of detectable contrast
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High throughput generation of water-in-oil emulsions for molecular genetic analyses

Researchers have developed a technology called BEAMing (Beads, Emulsion, Amplification and Magnetics), which can assess millions of molecules simultaneously for the study of human genetic variation (see invention number 4273 for more details). Following the development of BEAMing, JHU researchers made an improvement to the technique. In BEAMing, for every amplicon to be queried there needs to be a different emulsion. A new emulsion-making procedure allows for the creation of 192 different emuls
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Mouse Adult Kidney Stems Cells Modulate Function of Dendritic Cells

Abstract (Set) Recent data has suggested that stem cells, such as mesenchymal cells derived from bone marrow, demonstrate an immunosuppressive ability and could represent a new approach for the treatment of immune-related disorders and the regeneration of damaged tissue. Organ resident cells, on the other hand, have not been well studied with regard to regenerative or immunoregulatory capacity. JHU scientists theorized that kidney derived stem cells (KSC) could be used as a novel therapy after
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Novel Class and Clinical Application of Antithrombotic Agents that Specifically Inhibit Inflammation-related Vascular Occlusion

Inflammation is known to be an integral part of the vascular occlusive events that occur during heart attack and stroke, although the specific pathway that links inflammation to clot formation has not previously been determined. Using both in vitro and in vivo studies, JHU researchers have identified the specific components through which inflammation promotes clot formation. In addition, this inflammatory component of thrombus formation cannot be prevented by standard anti-thrombotic therapy. I
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Functional Cardiac Tissue Engineering Using Nanostructured Scaffolds for Cardiac Cell Therapy & High-Throughput Drug Screening

This technology incorporates a method to guide the three-dimensional growth and generation of functional cardiac tissues with controlled and enhanced electrophysiological properties using a nanostructured tissue scaffold. Ultraviolet (UV)-assisted capillary lithography or nano-imprinting technique is used to fabricate highly uniform nanopatterned substrates for large scale tissue regeneration on FDA approved biocompatible polymers. Biochemical and/or physical stimuli in the nanostructured scaffo
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Discovery of Novel HTLV-3 and HTLV-4 Retroviruses

Scientists at JHU have recently discovered the existence of two new types of Human T Lymphotropic Viruses (HTL-V), HTLV-3 and HTLV-4, which may be transmitted within humans. The HTLV-4 and HTLV-3 are serologically indistinguishable from existing HTLV-1 and HTL V-2 using current assays and this may explain why they had not been previously identified. Description (Set) Proposed Use (Set) The discovery of new types of HTL-V has important implications for future developments in HTL V diagnosis
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Epigallocatechin-3-Gallate (EGCG) Enhances CD8+ T Cell Mediated Anti-Tumor Immunity Induced by DNA Vaccination

Immunotherapy and chemotherapy are generally effective against small tumors in animal models of cancer, but many tumors can grow rapidly and become resistant to both therapies. JHU researchers have now shown that this resistance can be overcome by combining immunotherapy using DNA vaccination with chemotherapy using epigallocatechin-3-gallate (EGCG), a green tea extract. Remarkably, EGCG appears to augment the anti-tumor effects of DNA vaccine-mediated immunotherapy, resulting in a higher cure
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Alteration of the Albumin-bound Protein/peptide Complex (ABPPC) is Biomarker for Disease

JHU scientists have invented a cost-effective, rapid, and sensitive assay that characterizes proteins differentially bound to albumin in diseased and healthy patients and is compatible with current blood collection protocols. The current hypothesis states that albumin changes with disease, and therefore the complex of albumin and its bound proteins and peptides (ABPPC) changes. The ABPPC assay could measure a modification of albumin or a change in ABPPC composition (i.e. presence/absence of one
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Formation of Functional Motor units and Recovery from Paralysis Using Embryonic Stem Cell Transplantation

The technology describes the potential of embryonic stem cell-derived motor neurons to functionally replace those destroyed in a paralyzed rat model. JHU researchers have successfully demonstrated the ability to increase the success of transplanted axons extending out of the spinal cord into ventral roots using motor neurons derived from embryonic stem (ES) cells. Furthermore, transplant-derived axons reached muscle, formed neuromuscular junctions, were physiologically active, and mediated parti
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ATR-Seckel Cancer Cells for Drug Discovery

Advanced gene targeting methods were used to generate biallelic knock-in point mutations in human colorectal cancer cells that cause a loss of ATR function, but retain enough activity to permit viability. JHU researchers determined that progression of the cancer cell cycle after radiation treatment requires the ATR kinase. Description (Set) Proposed Use (Set) As ATR strongly contributed to clonogenic survival after radiation treatment, these data suggest that blocking ATR activity or activa
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Tumor Repressor Genes in the Prevention of Cancer

Some epidemiological studies suggest that many solid tumor types occur less often than predicted in individuals with Down syndrome. Using mouse models of trisomy 21 and cancer, scientists at JHU have provided biological proof of this phenomenon. In doing so, they propose to have identified a novel class of cancer related gene, a tumor repressor. Understanding how this class of gene represses tumorigenesis could provide the basis for developing prophylactic approaches to cancer prevention. Desc
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Sequence Motifs that Direct Nuclear Import of Small RNAs

Small RNA species, such as small-interfering RNAs (siRNAs) and microRNAs (miRNAs), have important roles in gene silencing and possess great potential for the development of novel therapeutics. Researchers at Johns Hopkins University have discovered the first known sequence element that regulates the behavior of siRNAs and miRNAs, a motif at their 3' termini that promotes nuclear import. Description (Set) Proposed Use (Set) This invention could be used to develop novel RNA interference-based
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Role of Kv Channels in Neuroregeneration and Protection

Injury to the human central nervous system (CNS) is usually difficult to treat because of the limited regeneration capabilities of the neurons. Evidence suggests that the lack of CNS regeneration is due to the presence of inhibitory factors in the CNS environment. While there is clearly a great need for development of compounds that prevent neuronal degeneration or that promote the growth of neurons, clinical trials of neuroprotective compounds for use in a variety of neurodegenerative diseases
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Vaccine to Prevent/Treat Esophageal Cancer in Individuals with Barrett's Esophageal Metaplasia

Esophageal cancer affects approximately 14,000 individuals per year in the United States, is increasing in incidence, and is associated with an exceptionally high mortality rate. The poor outcome in patients with esophageal cancer is reflective of both deficiencies in early detection - the disease is typically diagnosed at an advanced (unresectable) stage ? and the inadequacy of available standard therapies across stages. Compounding this high mortality rate is the rising incidence of esophageal
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Development of Peptide Antibody Depletion (PAD) and its Application to Mass Spectrometry Sample Preparation

One of the major limitations of mass spectrometry is obtaining mass information on proteins in the presence of contaminating materials or other high abundant proteins that, when present in the sample, suppress the observation of proteins of interest. JHU scientists have developed a novel application called Peptide Affinity Depletion (PAD) which specifically targets the high abundant, well ionizing proteins and/or their resulting peptides/fragments and removes them prior to the ionization procedu
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Embryonic Stem Cells from Animal Models of Motor Neuron Diseases Including Spinal Muscular Atrophy (SMA) and Amyotrophic Lateral Sclerosis (ALS)

Progress in the development of therapies for motor neuron diseases has been hampered by the lack of effective cell culture models. Since spinal motor neurons selectively die in ALS and SMA, an effective cell culture model must have mature motor neurons that have the genetic defect found in humans with these disorders and must have an identifiable phenotype that allows them to be distinguished from normal motor neurons in culture. This invention describes generation of embryonic stem (ES) cells f
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Processes for Regulating Blood Glucose in a Mammal and Novel Polypeptides Useful in Nad Biosynthesis

Scientists at JHU have discovered of a novel blood-circulating form of Nampt. This novel isoform a slightly higher molecular mass compared to the intracellular form of Nampt and is mainly secreted by brown adipose tissue or cells in mice. Scientists at JHU also have created a novel use for the HIB-1 B brown pre adipocyte cell line to produce the novel isoform. Description (Set) Proposed Use (Set) 1. NAD-related compounds (nicotinamide, nicotinamide mononucleotide, and NAD) and their deriva
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Phase 2 Inducers and Related Signaling Pathways Protect Cartilage Against Inflammation/Infection, Apoptosis and Stress

The molecular pathogenesis of arthritis associated with increased chondrocyte apoptosis is earmarked by over-expression of cyclooxygenase-2 (COX-2) in articular tissues. COX-2 inhibitors are efficacious in the treatment of osteoarthritis and rheumatoid arthritis but clinical findings of serious gastro-intestinal, renal and cardiovascular side-effects have resulted in some COX-2 inhibitors, such as Vioxx, being pulled from the market. JHU researchers have made the novel discovery that phase II in
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TC-1/A2

Scientists at JHU have developed an HLA-A2 expressing tumor mouse model that can naturally process and present E7 antigen via HLA-A2 molecules. Description (Set) Proposed Use (Set) This mouse model should be useful for assays of HLA-A2-restricted E7-specific antitumor responses and antitumor effects and serves as an excellent functional read-out for vaccine efficacy.
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A Rapid Assay Using Fine Needle Aspiration (FNA) to Test for 5-fluorouracil Dosing and Efficacy

Scientists at JHU have developed a functional assay for a class of widely used anti-cancer drugs, 5-fluorouracil and related compounds (5FU). The assay is based on Fine Needle Aspiration (FNA) of any neoplastic lesion from a patient being treated with 5FU, and processing of the material for thymidylate synthase (TS) analysis. The assay was developed to measure the degree to which TS is inhibited in tissue biopsies by 5FU and related drugs. The major novel aspect of this assay and utility is that
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The Use of Lumican as an Inflammation Modulator

This technology identifies an extra-cellular matrix protein, lumican, that plays a role in the inflammation pathway. This protein is present in the skin, cornea, intestines and blood vessels, and modulates its action through cell surface signaling molecules. Mice deficient in lumican have are resistant to bacterial lipopolysacharide (LPS) mediated septic shock and death. Synthetic peptides containing amino acid sequences from lumican could be used to modulate inflammatory responses in various ty
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Multiple Inversion Flip Flop (MIFF) Vectors

JHU researchers have developed novel Multiple Inversion Flip Flop (MIFF) plasmid vectors for controlling the expression of transgenes that are either stably-integrated or transient. These vectors comprise a cytomegalovirus (CMV) promoter flanked by LoxP sites in opposing orientations, which allow for reversible inversion of the promoter in the presence of the Cre recombinase, and the ability to turn gene expression on and off. The vectors also incorporate reporter transgenes encoding fluorescent
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SIV Clonal Isolates: SIV/17E-Fr, SIV/17E-C1, SIV/3-11RG, and SIV/3-7

Abstract (Set) JHU researchers have constructed infectious simian immunodeficiency virus (SIV) molecular clones that differ in their Env and Nef proteins. These clones display differences in neurovirulence and cellular tropism. Description (Set) Proposed Use (Set) SIV clones for studying cell invasion and neurovirulence, and modeling HIV infection
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Identification of E. Coli Determinants Contributing to Bacteremia that Form the Foundation for Development of Vaccines Against E. Coli Bacteremia Andsepsis

E. coli is the most common gram-negative organism causing nosocomial bacteremia and bacteremia in VA and elderly patients. The mortality and morbidity associated with E. coli bacteremia is substantial. For example, E. coli sepsis is associated with an estimated 40,000 deaths each year at a cost of two billion dollars in the US alone. At present there are no preventive measures for patients at risk of E. coli bacteremia. A major contributing factor is the incomplete understanding of microbial det
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Multifunctional Neck Brace

strengthening of cervical muscles and the prevention of atrophy of cervical muscles; and graduated mobility and improved comfort in patients with a disease attributable to the cervical region of the spinal column. All braces distribute the weight of the head more evenly to the base of the brace surface. Fully constructed prototypes include a cervical collar that allows for physician defined rotation, a cervical collar that has an adjustable distraction force that can be adjusted by either the pa
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Augmentation of Endocardial Thromboresistance

A critical function of the endocardium is to maintain local thromboresistance by providing an anticoagulant surface. Thrombomodulin, is expressed on the surface of the endocardium endothelial cells and is the key component of the anticoagulant protein C pathway, and thus is critical to maintaining vascular thromboresistance. Endocardial Thrombomodulin expression is significantly reduced by acute heart failure and leads to local microthrombus formation. Conversely, targeted restoration of Thrombo
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Cell Lines that Express SIV/HIV Receptors and Co-Receptors in the K562 Human Cell Line

Both simian and human immunodeficiency viruses (SIV and HIV) utilize chemokine receptors, with or without CD4, as portals for entry into susceptible cells. JHU researchers constructed a reporter cell line derived from non-susceptible human K562 cells, xKLuSIV, that can be used to readily identify cells that are infected through a straightforward, quantitative luciferase assay. This cell line was further modified to express the rhesus macaque CD4 and/or the rhesus chemokine receptor CCR5 protein
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HOXB7 as a Potential Prognostic Marker and Therapeutic Target in Cancer

Scientists at The Johns Hopkins University have determined that HOXB7, a member of homeobox superfamily of transcription factors, regulates the expression of EGFR/ER2 and ER?and functionally orchestrates their function in breast cancer cells. In addition, the amelioration of HOXB7 expression using siRNA in multiple breast cancer cell lines results in significant inhibition of tumor growth. This discovery suggests that HOXB7 could be used as a breast cancer prognostic marker and a therapeutic tar
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MEK KO Lines for Drug Discovery

MEK knockout in human colorectal cancer cell line HCT116. Description (Set) Proposed Use (Set) MAP kinase/ERK kinase (MEK) is the specific activator of extracellular signal-regulated kinase (ERK) and is part of a signaling pathway with a pivotal role in the control of cellular proliferation. The cell line described in this invention can be used in screens for novel molecules that target central pathway in human cancer cells or to confirm the specificity of drugs discovered through other app
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Line 1: Pkhd1flox3-4 mouse line, formal name Pkhd1tm1Ggg.; Pkhd1del3-4 mouse line, formal name Pkhd1tm1Ggg.1

Pkhd1flox3-4, when mutated, causes human autosomal recessive polycystic kidney disease (ARPKD). The Pkhdlde13-4 allele results after Cre-mediated deletion of exons 3 and 4. Homozygosity for this allele results in a wide range of abnormal phenotypes including ductal plate malformations of the liver, pancreatic cysts, respiratory distress, growth retardation, kidney cysts, and occasional vascular hemorrhages. Heterozygosity for this allele results in morbid obesity. Other studies in humans showed
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Neuroprotective Actions of Polyphenols, Flavonoid Derivatives and their Respective Extracts

Polyphenols, such as resveratrol, have been linked to many health benefits. This widespread belief stems from its intrinsic antioxidant properties that protect against oxidative damage. Recently, other data has suggested that polyphenols act as signaling molecules to regulate gene expression, the heme oxygenase-1 (HO-1) gene being one example. In addition, increased heme oxygenase activity has led to significant protection against models of in vitro and in vivo oxidative stress injury. Scientis
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NAC-1 Monoclonal Antibody

Scientists have developed a human NAC-1 monoclonal antibody. The antibody can be applied by immunohistochemistry, Western blot and immunoprecipitation to detect NAC-1 protein expression. Description (Set) Proposed Use (Set) This antibody has the potential to be used as a diagnostic reagent for cancer patients
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Estrogen Receptor in Normal (ERIN) Cells

There is no ideal system for studying pre-neoplastic cells that are ERpositive due to the inability to establish an estrogen growth stimulated but non-tumorigenic breast epithelial cell line. Scientists at JHU have developed independently derived clones from the non-cancerous MCF-10A human breast cell line that express ER and are growth stimulated by 17-beta-estradiol (E2) in the absence of epidermal growth factor (EGF), a cytokine normally required for MCF-10A cell proliferation. Description (
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Promoters for Neural Regeneration

Background Injury to the human central nervous system (CNS) is usually difficult to treat because of the limited regeneration capabilities of the neurons. Evidence suggests that the lack of CNS regeneration is due to the presence of inhibitory factors in the CNS environment. Myelin-associated Glycoprotein (MAG) is a major inhibitory molecule associated with myelin that actively restricts axon regeneration after injures to the CNS. Technology This invention describes a functional assay that deter
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Compounds with Effects on Potassium Channels

KCNQ channels play a role in cardiac and neuronal membrane excitability. Scientists at JHU have identified a series of novel compound that potently activates KCNQ channels. Initial studies of electrophysiology using whole cell recording and animal models revealed that the compound potentiates KCNQ current in transiently transfected chinese hamster ovary cells and native cells. Further molecular studies suggest the compounds act via different mechanisms discovered to date. Description (Set) P
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Inducing and Inhibiting T Cell Tolerance with A2a Receptor Agonists and Antagonists

T cells play a critical role in fighting disease and infection. However, when T cells recognize and attack normal or non-infected cells, autoimmunity ensues. A contrasting, but similarly detrimental situation is T cell tolerance, the state of antigen-specific unresponsiveness in the absence of immune system failure. In this instance, T cells become tolerant to infected or cancerous cells and recognize rather than kill them. If the gene/s responsible to T-cell tolerance could be determined, then
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The MR-eye: Methods and Devices for Local MRI

The present invention is a novel method to lock the MRI frame-of-reference to an internal device, where the RF excitation is confined to the immediate region surrounding the probe. Two dimensions are encoded using cylindrical encoding or conventional read/phase encoding. The third dimension is resolved along the body of the probe using one or more modification methods to provide optimum slice selection. These methods and devices provide real-time MRI scanning from the view-point of the probe. Pr
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Protein-Encapsulated Aligned Fibers by Electrospinning for Enhanced Peripheral Nerve Regeneration

We have developed novel protein-encapsulated fibrous meshes that are composed of micro-/nano-sized aligned fibers. The encapsulated protein in the fibers can be released in a sustained manner for up to several months while maintaining partial bioactivity. Direct application of these aligned electrospun fibers with and without growth factor encapsulation as nerve guide conduits has led to enhanced nerve regeneration across a 15mm critical defect gap in the sciatic nerve of a rat model. The succes
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MR-visible and radiopaque microcapsules for use as embolic agents and for immunoisolation of cellular therapeutics

Immunoisolation of cells through microencapsulation is a means to prevent their immune destruction following transplantation. To date, microencapsulation has shown great clinical potential for a wide range of diseases requiring enzyme or hormone replacement therapy, particularly diabetes. This invention describes methods of synthesizing radiopaque and MR-visible alginate-based microcapsule formulations for immunoisolation of cellular therapeutics, as well as methods of delivery and detection. Th
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A. phagocytophilum Monoclonal ab hybridomas

Researchers at JHU have created hybridomas producing monoclonal antibodies reactive with the obligate intracellular bacterium Anaplasma phagocytophilium 44kDa antigens (Msp2), AnkA and unidentified labile antigens. Description (Set) Proposed Use (Set) Useful tool for immunohistochemistry, immunoprecipitation, Western blot analysis, and immunocapture assays.
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Novel Compounds that Target Estrogen Receptor (ER) Positive. Tamoxifen-resistant Breast Cancers

The drug tamoxifen is widely used to treat estrogen receptor (ER) positive breast cancer and to prevent relapse. However, half of all recurrences in breast cancer patients are resistant to tamoxifen and often, more aggressive tumors result. Recently, researchers at The Johns Hopkins University, in collaboration with the University of Maryland, have described novel compounds that have potential use for the treatment of ER positive breast cancer, in addition to other estrogen-related conditions s
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Trioxane Dimers Having Surprisingly High Anticancer Activities and Surprisingly Long-lasting Antimalarial Activities

Artemisinin (1), a monomeric 1, 2, 4 trioxane, is usually administered for the treatment of multi-drug resistant malaria. However, studies have determined that artemisinin not only possesses excellent anti-malarial properties but also considerable anti-cancer activities. Dimeric trioxanes also possess potent anti-cancer activities and can inhibit the growth of prostate cancer C2H cells almost as effectively as Gemcitabine, a clinically used anticancer agent. Recently, scientists at JHU designed,
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Co-assembling Peptide Hydrogels

Peptide-based hydrogels have become popular for biomedical applications such as tissue engineering and drug delivery due to their biodegradable and bioresorbable properties. For use in vivo, hydrogels should assemble easily in a controlled fashion and be compatible with native entrapped biomolecules. A novel peptide-based hydrogel (which is 99.7 % water by weight) has recently been created that assembles by simple mixing of two peptide solutions. Novel features of this hydrogel include shear res
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Developing a High-throughput Screening Method for Identification and Validation of T Cell Epitopes Based Upon the Mass Spectral Analysis of Antigens Bound to Class II HLA-DR Constructs

All currently established methods for the identification of immunogenic epitopes are extremely tedious, time consuming and frequently unsuccessful. The identification of high throughput methods to isolate most likely candidates derived from natural processing of protein antigens is a most desirable goal. Scientists at JHU have invented a high throughput method to identify physiologically relevant epitopes presented by MHC class II for helper T-cell activation. Description (Set) Proposed Use
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A Fast, Cell-based, High-throughput Assay for the Identification of Compounds with DNA-interstrand Crosslinking Capability

Using somatic cell gene targeting, isogenic cell clones were created from the colorectal cancer cell line RKO. Each KO cell line differed only in the allelic status of one Fanconi Anemia (FA) gene, either FANCC or FANCG. For details, see publication by Gallmeier, E et al., 2006. Description (Set) Proposed Use (Set) DNA-interstrand crosslinking (ICL) drugs are a popular and effective treatment against cancer. Currently, there is a push to identify and develop novel compounds with ICL capabil
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Novel methods for genetic calcium channel blockade

The development of left ventricular hypertrophy (LVH), irrespective of etiology, confers an incremental risk of adverse outcomes in the general population and in patients with different forms of cardiovascular disease. Importantly, LVH is also an early event in patients destined to develop congestive heart failure. Calcium-signaling pathways have been implicated in the development of LVH, but the clinical use of pharmaceutical calcium channel blockers is limited by side effects due to blockade
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TGFbeta Antagonists, Including AT1 Receptor Blockers, Rescue the Multisystem Pathogenesis of Marfan Syndrome

This invention is based on the discovery that excessive activity of an important signaling protein, TGF?likely underlies a variety of problems in Marfan syndrome, including the tendency to develop emphysema. The many problems associated with increased TGF?ignaling can be rescued by TGF?ntagonism. The inventors demonstrated that excessive TGF?ctivation and signaling is central to the pathogenesis of Marfan syndrome, a common connective tissue disorder. Relevant phenotypes include emphysema due to
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Prostate Specific Membrane (PSMA)-binding Peptides Useful for Targeted Therapy for Cancer

Prostate-Specific Membrane Antigen (PSMA) is a glutamate carboxypeptidase II that is expressed by normal prostate epithelium and is even more highly expressed by a large proportion of prostate cancers and other tumor types. As PSMA is not expressed in the normal endothelium of non-prostate tissues, it represents an attractive candidate for selectively targeting therapies to prostate cancer and/or other solid tumors. JHU scientists have now identified a specific peptide that binds selectively to
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Peptide Substrates for Fibroblast Activation Protein (FAP) that can be Used to Generate FAP-activated Prodrugs and Protoxins

Fibroblast activation protein alpha (FAP-alpha) is a membrane bound serine protease that is only produced by reactive stromal cells present within sites of epithelial cancers. FAP-alpha is not expressed within normal stroma of any other adult tissues and therefore represents a potential target for epithelial cancer therapeutics. Researchers at JHU have identified and synthesized the first known peptide substrate for FAP that can be used to generate prodrugs and protoxins that are selectively act
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Antibody Based Anti-HIV-1 Microbicide Targeting a Host Protein

Development of microbicides to prevent HIV-1 transmission to women plays a valuable role in stemming the worldwide epidemic of the disease. In order to address some of the shortcomings of microbicides currently in advanced stages of clinical testing, scientists at JHU explored the development of antibodies to host cell adhesion molecules for use as anti-HIV-1 microbicide. They have identified an antibody targeting a host cell protein in which, in both in vitro and in vivo model systems, prevent
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Johns Hopkins University PROTEIN-DB2 Database System

Genomic data collection follows the workflow of amplification of DNA expression, amplified DNA extraction, high-throughput sequencing, and subsequent processing/analysis of the sequence data. DNA itself is a structurally stable molecule that can withstand cell lysis and remain relatively intact. Proteomic data collection, on the other hand, follows a more complex workflow, ranging from gel electrophoresis to mass spectrometry to liquid chromatography or a combination thereof. In addition, amplif
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Emission Ratiometric Kinase Activity Reports in High Throughput Drug Screening and Analysis

Protein phosphorylation is one of the major regulatory mechanisms controlling cellular functions and aberrant kinase activities are found to form the molecular basis for many disease phenotyes. As a result, protein kinases are well recognized as important drug targets and are a focus of small molecule drug discovery efforts. JHU inventors have developed improved emission ratiometric reporters as research tools for monitoring the activation and deactivation of a prototype kinase, cAMP-dependent k
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Chemical Control of Trk Signaling in Mice

Neurotrophins are a family of molecules that encourage survival of nervous tissue by secreting factors in a neuron's target field, and act by prohibiting the neuron from apoptosis. The neurotrophin family include nerve growth factors nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), and neurotrophin 4 (NT-4). There are two classes of receptors, p75 and the Tyrosine kinases. The Tyrosine kinases include TrkA, TrkB, and TrkC, and bind with specific neurotr
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Tetrahydrobiopterin for Treatment of Cardiac Failure and Remodeling

Tetrahyrdobiopterin (BH4) is an obligate cofactor for the aromatic amino acid hydroxylases (tyrosine, tryptophan, and phenylalanine), as well as for the nitric oxide synthases. It is currently used clinically in a subset of patients born with an inborn error of metabolism known as phenylketonuria (PKU). It has not been previously used as a clinical therapy to target nitric oxide synthase (NOS), but recent studies suggest this may be a very useful approach. When BH4 is oxidized and/or depleted, N
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Identification of Novel Putative Endogeneous Angiogenic Protein Inhibitors

The present invention identified over 70 novel putative endogenous angiogenesis inhibitors using methods of bioinformatics. Classification of these inhibitors is based on conserved domains of known anti-angiogenic fragments. Utilizing bioinformatics algorithms and searching the human protein database, novel inhibitors were identified. These newly identified proteins and protein segments constitute novel drug candidates for anti-angiogenesis therapeutics. Background Angiogenesis, the process of
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Web-Based Diabetes Data Management Application

Intensive control of blood glucose levels may delay the onset and progression of microvascular complications that contribute to the morbidity and mortality of patients with type 1 and type 2 diabetes melltus. Treatment regimens targeting intensive control rely on the administration of multiple doses of different preparations of insulin. These regimens are monitored and adjusted on the basis of frequent self-measured blood glucose readings. Patients who keep track of their own blood glucose meas
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Johns Hopkins Diabetes Management Program

The invention encompasses a team of health care professionals who directly manage the pharacologic, nutritional and educational components of the medical care plan for hospitalized patients with diabetes mellitus. The director of the team wil be a board certified endocrinologist, who wil be responsible for oversight, algorithm development and interfacing with other disciplines (administrative, nursing, pharacy, case managers etc). A nurse practitioner(s) wil provide the day-to-day management of
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New uses for old drugs: Identification of Hedgehog pathway antagonists previously tested in humans

Hedgehog (Hh) pathway activity is responsible for the growth of lethal cancers such as small cell lung cancer, and carcinomas of the esophagus, stomach, pancreas, biliary tract, and prostate, and account for as many as 25% of cancer deaths. Evidence for the critical role of continuous Hh pathway activity comes from the ability of cyclopamine, a potent antagonist of the Hh signaling pathway, to block growth in mouse models of these cancer types. Scientists at JHU have now identified a pathway an
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A New Method to Increase Memory T Lymphocytes and Enhance their Functions for Resistance of Cancer and Infections

CD137 has long been recognized as a co-stimulatory receptor for growth and functional maturation of recently activated T cells in the presence of T cell receptor signal. Now, researchers at JHU have discovered a new method to grow memory T lymphocytes and to stimulate their function. In addition, the invention describes that, in the absence of MHC and antigen, stimulation through CD137 on T cell surface selectively promotes proliferation of T cells with memory but not naive phenotypes, accompani
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pT2cfosGW

A current hypothesis is that sequences conserved over greater evolutionary distances are more likely to be functional than sequences that are less well conserved. In order to identify non-coding regulatory sequences, scientists at JHU developed a novel reporter vector based on the Tol2 transposon, and permitting reporter gene expression from a minimal promoter. Description (Set) Proposed Use (Set) This invention will facilitate identification of important functional elements in the DNA sequ
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Site-specific Modification of the Human Genome Using Custom-designed ZFNs

Scientists at JHU have designed sets of Zinc Finger Nucleases (ZFN) to target mouse genes, namely the tyrosinase (mTYR) and CFTR (mCFTR) and human genes, namely the CCR5 co-receptor (hCCR5) through which HN gains entry into cells early in the infection; the DMPK gene, which is involved in myotonic dystrophy; the CFTR gene, which is involved in muscular dystrophy; and �globin gene, involved in sickle cell anemia. Inverted sequences of the form (NNC/T) 3 or 4.. (G/ANN) 3or 4 separated anywhere
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Molecular Targets for the Anti-cancer Natural Product Pateamine A

v The natural marine product Pateamine A (PatA) is a potent anti-tumor and pro-apoptotic agent. Until recently, PatA?s mechanism of action was unknown until scientists at JHU determined that the protein inhibits cell proliferation through blockade of eukaryotic translation initiation. Specifically, PatA binds to and enhances the activity of eIF4A and causes changes in the affinity of eIF4A for its partner proteins, resulting in the stalling of initiator complexes on mRNA in vitro. In addition to
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S9

Cystic Fibrosis bronchial epithelial cell line isolated from a patient with genotype ?508 /W1282X. IB3-1 Cells were immortalized with adeno12SV0 and later infected with a viral vector AAV carrying CFTR full length. Description (Set) Proposed Use (Set) This cell line may be useful in the discovery of novel drug for the treatment of CF
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A Novel Statistical Method to Identify Robust Prostate Cancer Marker Genes

The most commonly used diagnostic screen for prostate cancer (measurement of levels of prostate specific antigen) is now known to function very poorly as a disease biomarker. The test developed in this work exhibits performance that dramatically exceeds that of PSA. The invention is the TSP (Top Scoring Pair) classifier algorithm, which is a robust method of integrating multiple sets of data from different DNA microarray studies. The system is used to find a set of markers with inverted expressi
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C38

JHU scientists have created a Cystic Fibrosis bronchial epithelial cell line that was isolated from a patient with genotype ?508/W1282X. Cells were immortalized with adeno12SV0 and later transfected with a cDNA from AAV carrying CFTR truncated for the first 119 amino acids. Description (Set) Proposed Use (Set) This cell line may be useful in the discovery of novel drug for the treatment of CF
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Interference of the mir-17 miRNA Cluster as a Novel Cancer Therapeutic Strategy

The MYC cancer gene increases the expression of a cluster of microRNAs that are implicated in the development of human cancers. Researchers at JHU have discovered that MYC regulates a growth regulator, E2F1, through several miRNAs. This novel discovery suggests that interference of E2F1 or other targets in the mir-17 cluster should inhibit cancer growth and development. Using antisense oligonucleotides to interfere with the miRNAs directed at E2F1, the scientists demonstrated that the level of E
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RAS KO Lines for Drug Discovery

RAS gene knockouts of the human colorectal carcinoma cell line, HCT116. Details are described in the paper by Parsons et al., 2005 Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the RAS pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or chemo preventive agents for specific cancers.
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Neuroprotection Actions of EP1 Receptor Drugs

Prostaglandin E2 is the primary product of arachidonic metabolism and is synthesized via the prostaglandin and cycloxygenase pathways. The hormone-like chemical has been shown to affect the central nervous system and can modulate synaptic transmission and neurotransmitter release, the sleep/wake cycle, fever, pain and the immune system. PGE2 can bind to four known G protein-coupled receptors, named EP1, 2, 3 and 4 that are distinguished based on their specific signal-transduction pathways. Rece
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A New Method for High-efficiency Integration of Transgenes into Bacterial Chromosomes without a Drug Marker

This invention allows the insertion of foreign DNA into an innocuous site in the bacterial chromosome using the site-specific recombination system of Tn7. Drug selection is not required since this integration event occurs in up to 97% of cells treated. The plasmid that carries this system is curable, once the process is complete all transposition machinery is lost, and only Tn7 and the transgene remain. Finally, this method is very easy to use and is suitable for use in environmental isolates of
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Arginase, a Target Enzyme for the Treatment of Myocardial Dysfunction in Aging and Heart Failure

We have identified a novel method to treat heart failure and age related myocardial dysfunction by inhibiting the enzyme Arginase. The seminal observations underlying this claim involve the following: We have demonstrated The expression of arginase I and II in rat heart and isolated cardiac myocytes, that arginase is upregulated in the hearts of aging rats, the inhibition of arginase increases myocyte contractility, and the enhanced contractility is mediated by a NOS-1 enzyme. Finally, we have
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Revolving Robotic Needle Driver

Abstract (Set) Image-guided robots are manipulators that operate based on medical images. These are modern medical tools with applications in most medical fields, allowing the physicians to perform interventions not only under direct vision, but with the additional help of the transcutaneous data. They have the potential to precisely target the disease or the intervention site in a percutaneous, minimally invasive, less traumatic, least disruptive fashion. Most needle robots position and/or ori
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Genome-wide Direct Transcription Measurement using Array-based Assay of Labeled Nascent RNA

Description (Set) JHU scientists have developed, optimized and validated an innovative approach for study of nascent RNA transcription. Post-transcriptional regulation of gene expression constitutes critical and wide-spread mechanisms. However, only ongoing transcription accurately reflects momentary regulatory events of transcriptional regulatory mechanisms. Ongoing transcription can be measured and distinguished from mRNA sequence turnover using Nuclear run-on (NRO) assays. Traditional NRO as
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Tandem L2 Fusion Vaccines for Prevention of Human Papillomavirus Infection

Abstract (Set) HPV infection causes 5% of human cancers worldwide. Prevention of HPV infection will eliminate HPV associated cancers and their precursors. However, current licensed vaccines are derived from Ll capsid protein and only target a subset of the oncogenic HPV types (and therefore Pap screening programs must remain in place). JHU scientists have identified a low cost approach to broadly prevent benign and oncogenic HPV infections and their sequelae based upon vaccination with polymeri
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L2 Lipopeptide Vaccine for Prevention of Human Papillomavirus Infection

Abstract (Set) Human papillomavirus (HPV) infections are the most common sexually transmitted infection in the United States. Infection by over 15 different oncogenic HPV genotypes is a cause of cervical cancer. Therefore, a low-cost, broadly protective vaccine that can be delivered without needles is urgently needed. JHU scientists have discovered a novel lipopeptide derived from the minor capsid protein L2 of medically-significant HPV genotype and produced by direct synthesis as an approach t
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Cardiac Stem Cell and Myocyte Secreted Paracrine Factors

Abstract (Set) Stem cells hold the promise to revolutionize future reparative medicine through the development of stem cell-based therapies. Transplanting stem cells (either embryonic or adult derived) into damaged myocardium is emerging as a novel means for acute repair and as an alternative to organ transplantation or ventricular assist devices in the treatment of end-stage heart failure. The JHU scientists identified unique stem cell-secreted paracrine factors, which can be useful therapeuti
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Imaging Methods for Assessment and Quantification of in vivo Antigen Capture and Vaccination

Abstract (Set) Immunization has eradicated many harmful infectious diseases in the Western world. This is commonly achieved by injecting individuals with attenuated pathogens such as viruses and bacteria. By using cancer vaccines, similar success will be achieved for eradicating the most common forms of cancer. However, as of today, significant hurdles exist to make this work. For successful immunization, it is mandatory that dendritic cells (DC), cells that initiate the immune response, take u
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Ras-regulated microRNAs as a novel anti-cancer therapeutic strategy

Abstract (Set) MicroRNAs (miRNAs) are 18-24 nucleotide RNA molecules that regulate the stability or translational efficiency of target mRNAs. miRNAs are frequently dysregulated in cancer cells and can act as both oncogenes and tumor-suppressors. It is estimated that there are approximately 37,170 new cases of, 33,370 deaths due to, and $1.5 billion spent on treatment of pancreatic cancer in the United States each year. Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human mali
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CisGenome Software

Abstract (Set) Chromatin immunoprecipitation followed by genome tiling array analysis (ChIP-chip) or by massively parallel sequencing (ChIP-seq) is an extremely powerful tool to understand gene regulatory programs in development and disease. CisGenome is a software system for analyzing genome-wide chromatin immunoprecipitation (ChIP) data. Developed by researchers at Johns Hopkins University and Stanford University, CisGenome software is designed to meet all basic needs of ChIP data analyses, i
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Technique for Identifying and Measuring Narp in Plasma

Abstract (Set) JHU scientists have developed a method of concentrating Neuronal activity regulated pentraxin (Narp) in plasma using taipoxin bound affinity beads. The concentrated Narp is detected using western blot methodology. The method can be used as a diagnostic tool for evaluating the physiological and pathologic states associated with psychiatric disorders, infection, and other diseases that cause hemodynamic change. Description (Set) Proposed Use (Set) Narp is co-released with the
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Mosquito-based Malaria Transmission-blocking Antibodies

Abstract (Set) A single effective vaccine against malaria is lacking. As such, the global research effort has focused on developing a "cocktail" vaccine, wherein different life stages of the parasite can be targeted specifically by separate vaccine components. Therefore, one vaccine component of interest would target the parasite stage (the ookinete) that invade the mosquito gut in the hope of blocking the cascade of secondary infections. The current paradigm involves developing antibodies agai
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Tumor Suppressor MicroRNAs Controlling Cancer Cell Growth

Description (Set) Johns Hopkins scientists have discovered two microRNAs that are expressed in normal blood cells, but are not expressed in virtually any of the leukemia-lymphoma cell lines and primary patient samples which have been tested to date. The miRNAs are not expressed in other types of solid tumor cell lines. Functional studies of these two microRNAs reveal that they control cancer cell growth and apoptotic state. Replacement of either of these microRNAs significantly slows the growth
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Method for Investigating Long-Range Chromatin Interactions in Vivo

Abstract (Set) The packaging of DNA into chromatin, the complex of DNA and protein that makes up chromosomes, is an important mechanism for regulating gene expression. Recent studies demonstrate that long-range chromatin interactions play an important role in transcriptional regulation of genes. Current techniques for investigating long-range chromatin interactions in vivo focus on physical proximities between chromatin elements without specific attention to identifying the protein components t
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Cost-Effective Method for High-Throughput and Quantitative Analysis of DNA Methylation

Abstract (Set) Investigators at Johns Hopkins University developed a simple and cost-effective approach to detect and quantify the status of DNA methylation, termed MSqFRET. This method couples the well established methylation-specific PCR (MSP) technology with quantum dot-mediated fluorescence resonance energy transfer (FRET) DNA sensing. The methylation status of genomic DNA present in a specimen is analysed by treating the specimen with sodium bisulfite that modifies unmethylated cytosine, a
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http://www.jhttportal.jhu.edu/TechTransfer/TechnologyLocator.asp

Description (Set) JHU inventors have identified a population of microRNA (miRNA) associated with a critical neurotransmitter transporter gene regulatory region. miRNA has roles in modulating gene expression levels. Transporter activity is essential to maintain proper neurotransmitter levels in the nervous system. Multiple neurological diseases have been linked to abnormal transporter activity and neurotransmitter levels. JHU inventors determined that at least one of the miRNA levels are elevate
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Novel Antibiotic Treatment of Gliosarcoma Reduces Tumor Size and Increases Survival

Abstract (Set) The present invention is directed to methods of using a bacteriostatic antibiotic to treat glioma and other brain tumors including astrocytoma, oligodendroglioma, glioblastoma multiform but also other cancer types including breast cancer, cervical cancer, AIDS related cancer, gastric cancer, head and neck cancer, prostate cancer and else. In particular, the bacteriostatic antibiotic or a pharmaceutically-acceptable salt thereof, is administered to a subject for a time and in an a
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LRRK2 Fly Model for Parkinson's Disease

Abstract (Set) Mutations in the leucine rich repeat kinase (LRRK2) gene causes late onset autosomal dominant Parkinson's disease (PD). The LRR protein has two enzyme activities (kinase and GTPase) which play important roles in regulating neuronal death and other cellular functions. We have created WT and GS LRRK2 fly models that recapitulate several key features of PD including loss of selective neurons, locomotor dysfunction and early death. Description (Set) Proposed Use (Set) This is th
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Methods to Reduce Mesh Spacing of the Protective Mucus Barrier

Description (Set) Human mucus forms a protective barrier to toxins and infectious pathogens on all the most susceptible surfaces of the body such as the lung airways, gastrointestinal tract, nose, eye, and female reproductive tract. Mucus possesses a mesh-like structure which evolved viruses can bypass. The viruses are sufficiently small as to diffuse through the protective mucus barrier at rates similar to that of water, thus eluding the protective barrier actions of mucus. Johns Hopkins scien
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The Method to Enhance Death of Cancer Cells

Abstract (Set) B7-H1 is an immunoglobulin-like immune suppressive molecule broadly detectable on the majority of human and rodent cancers and its functions have been attributed to delivering an inhibitory signal to its counter-receptor programmed death-1 (PD-1) on T cells. JHU scientists have discovered a novel mechanism of action by which cancer escapes from therapeutics, leading to the establishment of a new target on cancer cells to enhance death of cancer cells. B7-H1 on cancer cells receiv
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Radiolabeled Analogs of Cyclopamine for Imaging Hedgehog Signaling

Description (Set) The hedgehog (Hh) signaling pathway is increasingly recognized as an important therapeutic target in cancer. When Hh signaling goes awry patients may develop a wide variety of tumors including medulloblastoma, glioblastoma, pancreatic cancer, prostate cancer, among others. In addition, targeting the Hh pathway with inhibitors such as cyclopamine has been shown to deplete tumors of their stem cell population thereby inhibiting their growth. Cyclopamine binds to the transmembran
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Immunoliposomal Delivery of Vaccine to the Spleen

Abstract (Set) A fundamental problem in all vaccination methods is to present the target antigen to the appropriate population of cells so that the immune system will be sufficiently activated to mount an immune response against cells that exhibit the target antigen. The problem in many cases is that the immune system is not sufficiently activated, and the immune response is of short duration or inadequate to eradicate the antigen-positive cells. It is thought that this is, in part, because den
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Molecular Insights into Breast Cancer Prevention and Therapeutics Involving Novel Genistein Analogs

Abstract (Set) Cancer is the second leading cause of death in the U.S. and claims the lives of around 555,000 Americans each year. An estimated 1.3 million new cases of cancer are reported in the U.S. each year, of which breast cancer accounts for 15.3 percent. In Asia, an area with high soya isoflavone intake, breast cancer rates are lower than in the western countries. The potential estrogenic properties of genistein and other isoflavones have raised some concerns about their use in women at
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Rapid Induction of Cancer Cell Death by Synergistic Treatment of Tetra-O-methyl Nordihydroguaiaretic Acid (M4N) with either Two Specific Kinase Inhibitors

Abstract (Set) M4N is an anti-cancer drug candidate, which is currently under the first phase of clinical trials. Although M4N can reduce tumor cell growth; it fails to induce quick tumor cell death. JHU scientists showed that the combination treatment of M4N with either two specific kinase inhibitors overcomes this problem. In vivo study demonstrated that the combination treatment reduced tumor metastasis and extended life expectancy of tumor-bearing mice significantly. This combination treatm
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Dually Targeting the PI3K/Akt and MAP Kinase Pathways in Poorly Differentiated and Undifferentiated Thyroid Cancer Cells has High Therapeutic Promise

Abstract (Set) JHU scientists have discovered an innovative method of targeting aggressive thyroid cancers that are resistant to conventional treatment. Poorly differentiated anaplastic thyroid cancer cells do not respond to radioiodine treatment due to abnormal silencing of iodide-metabolizing genes. Recent studies suggest that two signal transduction pathways have critical roles in expression and activity of the iodide-metabolizing genes. JHU scientists found that modulation of both the PI3K/
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Detection of Virus Exposure as an Etiological Agent in Patients with Sudden Onset of Psychosis

Description (Set) JHU scientists have developed an assay based on detection of certain viral antigens in patients with a sudden and unexplained onset of psychiatric symptoms. This discovery is rooted in the premise that some viruses are implicated as etiological agents in neurological conditions. JHU scientists determined a significant correlation between onset of sudden psychosis and increased rates of exposure to pathogenic viruses. JHU scientists found that certain psychoses appear to be cor
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Salivary Rinse and Serum Detection Test for Head and Neck Cancer

Description (Set) A Johns Hopkins University investigator has identified unique profiles of hypermethylated gene promoters that are effective markers of risk for head and neck cancer. Head and neck squamous cell carcinoma (HNSCC) is a disease that is often diagnosed at an advanced stage. The resulting delay in treatment contributes to increase morbidity. The present invention is an analytical test for early detection of HNSCC based on assessing gene specific promoter hypermethylation in serum a
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Beta-3 Adrenergic Receptor Agonists Reduce Cardiac Hypertrophy and Heart Failure

Abstract (Set) This invention is based on the discovery of a novel role for beta-3 adrenergic receptors (B3AR) in the control of cardiac hypertrophy when the heart is under physical stress. The precise role of B3AR in the heart has not been previously elucidated. In general, beta-1 and -2 adrenergic receptors are activated when the heart is under stress (?fight or flight response?); however, activation of B3ARs have an opposite effect. B3AR are increased in human heart failure as a protective m
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Cancer Immunotherapy Using Irradiated Tumor Cells Secreting Hsp 70

Abstract (Set) Ovarian cancer is responsible for the highest mortality rate among patients with gynecologic malignancies. Therefore, there is an emerging need for innovative therapies for the control of advanced ovarian cancer. Immunotherapy has emerged as a potentially plausible approach for the control of ovarian cancer. JHU scientists have generated heat shock protein 70 (Hsp70) secreting murine ovarian cancer cells that express luciferase (MOSEC-Iuc). They also characterized the antigen-spe
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Novel Cell Surface Receptor Variants as Biomarkers for Hormone-Refractory Human Prostate Cancer

Abstract (Set) Prostate cancer cells depend on hormones and hormone receptor activity for growth and survival. Some prostate cancer cells progress to a phenotype that is aggressive and no longer susceptible to hormone therapies that are standard treatments for prostate tumors. JHU scientists have identified novel cell surface receptor gene variants that are specifically expressed in hormone-refractory prostate cancer cells present in primary clinical samples from prostate resections and radical
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High Affinity Small Molecule Inhibitors of Hepatitis C NS3/4A Protease

Description (Set) Hepatitis C virus (HCV) infection is a growing global health problem with over 3 million new cases of HCV infection each year. Johns Hopkins University scientists have discovered a new class of small molecule non-peptidic inhibitors of the HCV NS3/4A protease. The HCV NS3/4A protease is an important serine protease that plays an essential role in the processing and maturation of non structural proteins in the virus and has been validated as an important target for HCV drug dis
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Monoamine oxidase (MAO) inhibitors reduce cardiac hypertrophy and prevent heart failure

Description (Set) JHU scientists have developed a method to reduce heart muscle cell changes that lead to heart enlargement and congestive heart failure, using agents that inhibit monoamine oxidase (MAO) activity. MAO functions in normal metabolism as an oxidizing enzyme and is present in cardiac muscle cells. JHU scientists determined that increased MAO activity directly contributes to changes in cardiac muscle cells that result in abnormal heart enlargement (cardiac hypertrophy). MAO inhibito
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Novel Peptide and Delivery Method for the Treatment of Human Cancers

Description (Set) The PIK3 signaling pathway has recently been recognized a major player in the regulation of cell survival and cell proliferation as it relates to cancer. Scientists at JHU have utilized a small peptide based upon a component in this pathway to block cell proliferation and cell cycle progression in many cancer cell lines as well as in vivo tumor models. In addition to an earlier-developed adenovirus delivery system, these scientists have recently generated a fusion peptide that
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A Predictive Test for Determining Chemotherapy Regimens in Patients with Cancer

Abstract (Set) The ability to predict sensitivity to individual cytotoxic chemotherapies could improve treatment response and survival of cancer patients. There is currently no method in routine use to determine sensitivity to agents directed at the microtubule, and particularly the taxanes. JHU scientists examined DNA methylation of a specific gene in esophageal cancer patients, and found that methylation of this gene is associated with achieving a pathologic complete response only in patients
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Carcinoembryonic Antigen as a Potential Biomarker for use in Targeted Drug Delivery Applications as well as Other Therapeutic Strategies

Abstract (Set) Blood-borne metastasis is a highly regulated and dynamic process, whereby cancerous cells separate from a primary tumor, migrate across blood vessel walls into the bloodstream, evade host defenses, adhere to the vascular endothelium of distant organs, extravasate and colonize these sites. JHU scientists have identified that carcinoembryonic antigen, expressed by colon carcinoma cells, possesses specific selectin binding activity. This fact provides the basis to engineer novel the
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Mutational Analysis of the Serine-threonine Kinome in Colorectal Cancers

The invention describes novel mutations in serine-threonine kinase genes within the Phosphatidylinositol-3-OH Kinase (PI(3)K) pathway in patients with colorectal cancer. Details are described in the paper by Parsons et al., 2005 (see below). Description (Set) Proposed Use (Set) The discovery of novel mutations in PI(3)K pathway genes provide a basis for the development of new therapeutic and diagnostic approaches for such patients.
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Mouse Monoclonal Antibody 8A2

Abstract (Set) This technology is a mouse monoclonal antibody, which specifically recognizes vertebra SUMO-2 and SUMO-3. SUMOylation is essential for cell-cycle regulation in invertebrates; however, its functions during the mammalian cell cycle are largely uncharacterized. Mammals express three SUMO paralogs: SUMO-1, SUMO-2, and SUMO-3, the specific localization of which was identified by JHU scientists. Since, SUMO paralogs regulate distinct mitotic processes in mammalian cells, global inhibit
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Modulation of Regulatory T cell (Treg) Function

Abstract (Set) Regulatory T cells (Treg) are a subset of the CD4 T cell population that down-modulate an immune response. In some diseases, i.e. autoimmunity, Treg activity can prove beneficial by inhibiting a pathological response. However, under other conditions, including but not limited to chronic infection or cancer, Treg activity prevents an effector immune response that might clear the infectious organism or tumor. Under those conditions, inhibition of Treg activity would be desirab
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Instrument System for Managing and Cherry Picking Microorganism Collections

Large biomaterial collections require precise and selective access to a subset of material under sterile conditions. JHU researchers have invented a novel integrated instrument system built from existing standard components that allows efficient management of large, complex libraries of biomaterials. The invention will allow easy assess to stored biological materials and efficient retrieval of a user-defined subset, for further experimental manipulation. The specific application involves withdra
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Short Chain Fatty Acid (SCFA) - Carbohydrate Bifunctional Hybrid Molecules with Novel Anti-cancer Properties

Butyrate, a natural short chain fatty acid (SCFA), is well-established to have anti-cancer properties via its ability to induce cell cycle arrest and apoptosis in cancer cells. Currently, several clinical trials are underway for treatment of cancer (leukemia, prostate, sickle cell ulcers and liver disorder) with butyrate and its analogs. There have been several attempts to develop prodrugs for butyrate in the form of esters with innocuous carrier molecules like lactic acid, glycerol, glucose, ma
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Hi-Throughput Assay for Screening Small Molecule Inhibitors of Uracil DNA Glycosylase

Uracil DNA glycosylase (UDG) is one of the primary enzymes that prevents the incorporation and accumulation of deoxyuridine in genomic DNA. The identification of pharmacologic agents that inhibit UDG is desirable for both investigational and therapeutic purposes. JHU scientists have developed a novel screening assay that allows for the screening of UDG enzymatic activity using sensitive molecular beacon technology. The assay is especially robust and economical for high-throughput screening appli
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Total Human Serum Free IgE Immunoenzymetric Assay (IEMA)

Xolair (generic name, omalizumab) is currently approved by the FDA for the treatment of extrinsic asthma. Presently, there are no serological markers or assays for monitoring the efficacy of prolonged Xolair therapy in patients with extrinsic asthma. Monitoring the free IgE level in the blood is one indicator of Xolair efficacy since the drug's action is expected to decrease the "free" IgE by greater than 95%. Scientists at JHU have now developed a novel assay to determine the efficacy of Xolai
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Large-scale Survival, Differentiation and Structural Integration of Human Neural Stem Cells Grafted into the Adult

The present invention provides methods and compositions for treating spinal cord diseases and injuries. The methods involve transplanting neural stem cells which have been previously expanded in vitro into a patient such that the cells can ameliorate the disease or injury. The stem cells to be transplanted are derived from spinal cord tissue. Patent (Set) WO 2006/135899
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Inhibition of Ischemia-induced Arrhythmias by Blocking Mitochondrial Inner Membrane Ion Channels

This invention describes a method for preventing and/or terminating ischemia-related arrhythmias by targeting the mechanism responsible for the electrical instability occurring during an ischemic episode or upon reperfusion of the heart. Inhibition of a mitochondrial inner membrane anion channel, either directly or indirectly through the mitochondrial benzodiazepine receptor, prevents or reverses mitochondrial depolarization under metabolic stress. Reduced mitochondrial depolarization leads to r
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Novel Drugs for the Treatment of Optic Nerve Disease

A small molecule library was screened using an RGC cell culture system for novel factors that promote RGC survival and outgrowth. Five novel compounds have been identified that promote the development of neuritis and/or survival of cultures of retinal ganglion cells. These compounds are beneficial in the treatment of a variety of optic nerve disease including glaucoma, optic neuritis, and ischemic optic neuropathy. Problem Solved/Background Retinal ganglion cells (RGC) are the projections neuron
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Method for identifying mutations in coding and non-coding DNA

Annotation of the completed human genome has demonstrated that about 1.5 % encodes protein. About twice (roughly 3.5%) falls within non-coding sequence and is suggested to be functional based on extensive (>50nt) sequence conservation. The remainder of the genome consists of non-coding DNA with lesser sequence conservation. However, this genomic compartment may still contain small regions of functional sequence that harbor mutations important for human disease. Thus, JHU scientists have develope
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Gowth of Mesenchymal Stem Cells Under Non Adherent Conditions for Clinical Applications

The use of bone marrow derived mesenchymal stem cells (MSC) has been proposed for a number of regenerative therapies including repair of myocardial tissue. The data to date has suggested improved functional outcomes but have failed to demonstrate incorporation of MSC into the tissue. These studies have utilized MSC grown as adherent cells in plastic tissue culture flasks and trypsinized for harvest and infusion. Scientists at JHU have now developed methodologies that allow generation of MSC in
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Immortalization of Nociceptive Dorsal Root Ganglion Sensory Neurons

This invention involves a novel method to immortalize nociceptive dorsal root ganglion (DRG) neurons. DRG neurons are terminally differentiated cells that extend long axons to their target tissues. More than half of all DRG neurons are unmyelinated axons that extend to the skin and have nociceptive properties. In many studies on peripheral neuropathies and neuropathic pain, rat or mouse primary DRG neurons are used. However, obtaining these cells in sufficient quantities to perform high-throughp
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Neuropilin-1 Sema Mice

Neuropilin-1 (Npn-1) is a receptor that binds ligands from structurally distinct families like semaphorins (Sema) and vascular endothelial growth factors (VEGF). Npn-1 is a type I transmembrane protein with a small cytoplasmic domain and multiple extracellular domains capable of mediating a variety of protein/protein interactions. Studies using transgenic and knock-out mice of NPN-1 indicate that this molecule is important for vascular and neuronal development. Gene replacement strategy was used
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Reactive Oxygen Generating Enzyme Inhibitor with Nitric Oxide Bioactivity and Uses Thereof

In the US, CHF is the number one cause of hospitalization in people over age 65, and it affected approximately 5 million people in 2004. This translates into a potential cost of $29 billion for the healthcare system. In the US over half a million new cases are diagnosed with, and over 50,000 individuals die from CHF each year. Current treatments are only moderately effective, first year mortality rates for CHF are comparable to those of many cancers. The CHF drug market is expected to approach $
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A Compression Device for Enhancing Normal/Abnormal Tissue Contrast in MRI

This invention is a pneumatic device for detecting breast cancer with MRI. The device enables imaging of the elasticity (stiffness) of the tumor and mechanical properties of the surrounding tissue through the use of the SENC (Strain Encoding Imaging) spectrum. The SENC technique also allows for real-time quantification of tissue deformation. Additionally, the device improves the resolution of the images with contrast agents due to the applied pressure on the area of interest. The device differs
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Inhibiting Binding of Inducible Nitric Oxide Synthase to Cyclooxygenase-2 to Treat Inflammation

Cyclooxygenase (C0X2) and inducible nitric oxide synthase (iNOS) are two major inflammatory mediators. Inducible NOS specifically binds to C0X2 and S-nitrosylates it, enhancing C0X2 catalytic activity. Selectively disrupting iN0S-C0X2 binding prevents NO-mediated activation of C0X2. The synergistic molecular interaction between two inflammatory systems permits assays for developing anti-inflammatory drugs. Patent (Set) WO 2006/116702
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Targeting of Stat-3 Signaling in the Hematopoietic System Evokes Multicomponent Therapeutic Antitumor Immunity

The immune system can act as an extrinsic suppressor of tumors. Therefore, tumor progression depends in part on mechanisms that down modulate intrinsic immune surveillance. Identifying these inhibitory pathways may provide promising targets to enhance antitumor immunity. Scientists at Johns Hopkins University and the University of Florida have shown that Stat3 is constitutively activated in diverse tumor-infiltrating immune cells, and ablating Stat3 in hematopoietic cells triggers an intrinsic
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HAUSP KO Lines for Drug Discovery

HAUSP (also known as USP7) deubiquitinase gene knockouts of the human colorectal carcinoma cell line, HCT116. Details are described in the paper by Cummins et al., 2004a (see below) Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the p53 pathway in human cancer cells or to confirm the specificity of p53-targeted drugs discovered through other approaches.
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XIAP KO Lines for Drug Discovery

X-linked inhibitor of apoptosis protein (XIAP) gene knockouts of human colon cancer cell lines. Details are described in the paper by Cummins et al., 2004 (see below). Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the mitochondrial death pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or che
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DEC1 KO Lines for Drug Discovery

Deleted in esophageal cancer 1 (DEC1) gene knockouts of the human keratinocyte cell line, HaCaT. Details are described in the paper by Zawel et al., 2002 Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the TGF-Beta pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or chemopreventive agents.
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CHK2 KO Lines for Drug Discovery

Chk2 protein kinase gene knockouts of the human colorectal carcinoma cell line, HCT116. Details are described in the paper by Jallepalli et al., 2003 (see below). Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the CHK2 pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or chemopreventive agents.
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RAF KO Lines for Drug Discovery

BRAF kinase gene knockouts of human cancer cell lines. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the RAF pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or chemopreventive agents.
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Dicer KO Lines for Drug Discovery

Dicer RNase gene knockouts in human cell lines. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the miRNA pathway in human cancer cells or to confirm the specificity of siRNA-based drugs discovered through other approaches.
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PIK3CA KO Lines for Drug Discovery

PIK3CA knockouts in human colorectal cancer cell lines HCT116 and DLD1. Details are described in the paper by Samuels et al., 2005 Description (Set) Proposed Use (Set) JHU scientists have recently discovered that mutations in PIK3CA occur in a significant fraction of cancers. Knockout lines indicated that PIK3CA plays a role in tumor cell proliferation as well as invasion and metastasis. The PIK3CA knock out cell line can be used in screens for novel molecules that target the PI3K pathway i
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SMAC KO Lines for Drug Discovery

Second mitochondrial-derived activator of caspase (SMAC; also known as Diablo) gene knockouts of the human colorectal carcinoma cell line, HCT116. Details are described in the paper by Kohli et al., 2004 Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the mitochondrial death pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially
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p16 KO Lines for Drug Discovery

p16 tumor suppressor gene knockouts of the human colorectal carcinoma cell line, HCT116. Details are described in the paper by Bachman et al., 2003 Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the DNA methylation or the p16 pathway in human cancer cells or to confirm the specificity of drugs discovered through other approaches. Such drugs can potentially be used as chemotherapeutic or chemopreventive a
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PTEN KO Lines for Drug Discovery

PTEN is a tumor suppressor gene that is frequently mutated or deleted in a variety of human cancers . PTEN functions primarily as a lipid phosphatase and plays a key role in the regulation of the PI3 kinase/Akt pathway, thereby modulating cell proliferation and cell survival. JHU researchers have created PTEN knock out cell lines. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the PTEN pathway or to va
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MTAP KO Lines for Drug Discovery

Methylthioadenosine phosphorylase (MTAP) plays an important role in the salvage pathway for the synthesis of adenosine. The loss of MTAP function has been implicated in a variety of cancers. JHU researchers have created MTAP knock out cell lines. Description (Set) Proposed Use (Set) The cell lines described in this invention can be used in screens for novel molecules that target the deficiency of MTAP
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Selectively Targeted Histone Deacetylase Inhibitors

Inhibitors of histone deacetylases (HDAC) are regarded as an important approach to cancer chemotherapy, since they disrupt the aberrant cellular metabolism that occurs in many tumor types. The HDAC inhibitors currently in clinical trials and typified by compounds such as trichostatin (TSA) and MS-275, are effective antitumor agents, but also produce significant toxicity to cells and whole animals. JHU researchers have designed and synthesized 16 compounds which are hybrids including structural a
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Use of Consensus Sequence as Vaccine Antigen to Enhance Recognition of Virulent Viral Variants

Vaccines for diverse viruses will be most effective when they direct the recipient?s response to recognize the most virulent form of the organism. Scientists at JHU have found that circulating strains of hepatitis C virus (HCV) contain substitutions that render the virus less fit. Therefore, the use of a consensus sequence as a vaccine for HCV would stimulate responses that drive the virus to mutate to a less-fit state, thereby rendering it less able to replicate and easier to eradicate. Further
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Generation of Isogenic Lines Differing Only in MLH1 Status

JHU researchers have created matched cell lines which are isogenic, differing only in the MLH1 alleles. One line has two defective alleles, the other has one defective allele and one normal (wild-type) allele, which was generated by targeted homologous recombination ("knock-in"). One wild-type allele is sufficient to generate normal mismatch repair. Description (Set) Proposed Use (Set) The cell lines can be used to test for compounds that are specifically toxic for cells lacking mismatch r
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A Novel Genetic Approach to inhibit or Eliminate the Tumorgenicity of Human Embryonic Stem Cells and/or their Derivatives After Transplantation

Pluripotent embryonic stem cells have enormous potential for providing an unlimited supply of multipotent and specialized cells, such as brain and heart cells, for transplantation and cell-based therapies that are otherwise limited by donor availability. However, this promising application is hampered by concerns that embryonic stem cells or their multipotent derivatives also possess the potential to form malignant tumors after transplantation in vivo. JHU researchers have conceived a novel meth
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B(subscript 1)-Insensitive T(subscript 2) Magnetization Preparation for Magnetic Resonance Imaging at High Field

T2 magnetization preparation is a commonly used MRI technique that enhances the contrast between arteries and surrounding tissues. Recently, high field imaging has shown promising results in acquiring high signal-to-noise images with sufficient spatial resolution suited for clinical diagnosis. However, the standard T2 magnetization preparation technique results in imaging artifacts due to non-uniform radiofrequency (RF) field across the imaging volume leading to a non uniform response, particula
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Identification of PSCA as an Immune Relevant Target of Antitumor Immune Responses

Prostate Stem Cell Antigen (PSCA) was previously identified as a biomarker for pancreatic and prostate cancer. JHU researchers have shown that a pancreatic cancer vaccine induced immune responses against PSCA in patients who demonstrated a positive clinical response, identifying PSCA as a candidate tumor antigen for immune-based therapies. This antigen may serve as a vaccine target, as a target to generate T cells for adoptive therapy, and as an antibody target for diagnosis and treatment of PSC
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New Analogs of the Hormone 1(alpha)25-Dihydroxyvitamin D with Side Chain Carbonyl Groups

Medicinal chemists are designing analogs to be at least as potent as, but less calcemic than, the natural hormone la, 25-dihydroxyvitamin D3 (calcitriol) for chemotherapy of diverse human illnesses. Scientists at JHU have previously shown that some new vitamin D analogs, even though lacking the classical side-chain terminal OH group, are transcriptionally active and low-calcemic. They have now created a new series of calcitriol analogs that do not possess the 16, 17-unsaturation characteristic t
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Strategies for Designing Drugs that Target the Sir2 Family of Enzymes to Treat Multiple Diseases

Sir2 enzymes comprise a unique class of NAD+ -dependant protein deacetylases that are involved in a broad range of biological processes including transcriptional silencing, aging, fat mobilization and regulation of the p53 tumor suppressor gene. The Sir2 proteins catalyze NAD+ -dependant acetylation of lysine at the critical site known as the C-pocket, releasing the deacetylated peptide, nicotinamide, and O-acetyl ADP-ribose. A part of the C-pocket is formed by the flexible loop region that ado
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High Fidelity DNA Display for the Evolution of Combinatorial Libraries

This invention relates to a method of producing combinatorial libraries from non-biological molecules coupled with replication of selected compounds. Individual molecules used to construct the library are attached to specific DNA sequences that are assembled as polymers, and so each combinatiorial compound has a DNA template that provides a set of instructions for its synthesis. Therefore, this technology allows non-biological compounds to undergo iterative rounds of screening and selection in o
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Platelet Endothelial Cell Adhesion Molecule-1 Gene Polymorphism and its Soluble Level are Associated with Severe Coronary Artery Stenosis in Chinese and Asian Indians

Many established risk factors in atherosclerosis and Coronary Artery Disease (CAD) have been defined for Caucasians but not for other ethnic groups. For example, the Asian Indians have 4 times higher prevalence of CAD compared to Caucasians. Thus, there is an urgent need to find novel risk factors/gene polymorphisms in Asian patients that may lead to rapid diagnosis and facilitate drug discovery. During research by JHU scientists, a strong correlation was discovered between the prevalence of cer
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High Fidelity DNA Display for the Evolution of Combinatorial Libraries

This invention relates to a method of producing combinatorial libraries from non-biological molecules coupled with replication of selected compounds. Individual molecules used to construct the library are attached to specific DNA sequences that are assembled as polymers, and so each combinatiorial compound has a DNA template that provides a set of instructions for its synthesis. Therefore, this technology allows non-biological compounds to undergo iterative rounds of screening and selection in
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Function of GDE Protein Family in Cellular Differentiation

This technology describes a novel retinoid-inducible gene, GDE2 (glycerophosphodiester phosphodiesterase 2) that encodes a 6 TM protein and is necessary to drive spinal motor neuron differentiation in vivo. A single amino acid mutation in the extracellular catalytic domain removes protein function and reveals a critical role for glycerophosphodiester metabolism in motor neuron differentiation. Background During neural development, regulation of cell-cycle and differentiation is essential for cel
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PDE Inhibitors in Immunotherapy

Defects in the immune system of tumor-bearing hosts can dramatically preclude effective immune-therapy. JHU researchers and others have previously described some of the cellular and molecular mechanisms responsible for these defects and have shown that the inhibition of these pathways can facilitate immune-mediated eradication of cancer. Specifically, immune dysfunction found in tumor-bearing animals as well as in cancer patients may be associated with the recruitment by the tumor of myeloid su
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A method for high-efficiency clonal isolation and expansion of human embryonic stem cells

JHU researchers have developed a method and identified growth factors for high efficiency cloning, clonal expansion and subculture of human embryonic stem (hES) cells. The invention further describes a method for rapid, high-throughput screening (including genetic screening) for agents (genes, growth factors, chemicals) that regulate all aspects of human embryonic stem cell growth including, but not limited to, cell adhesion, cell survival, cell proliferation, self-renewal and differentiation.
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Facile Modification of Collagen Directed by Synthetic Peptides

mechanical properties. Such modification may allow attachment of bioactive agent that can bring in designed bioactivity to natural collagen. Most of the techniques practiced today for collagen modification involves simple "blending method" which results in weak attachment of the target molecules or "chemical modification" that requires relatively harsh reaction conditions that are inapplicable to modifying collagens in living tissues. JHU researchers have developed a novel, simple approach for m
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Method for the direct extraction of isothiocyanates into the seed oil from glucosinolate-containing plant seeds

JHU researchers have developed a methodology for simple, reliable, stable, and inexpensive incorporation of plant isothiocyanates into oils such as natural plant seed oils. Some plant isothiocyanates are cancer chemoprotective, antibiotic, or insect repellents. These oils are ideal for topical dermal application. Description (Set) Proposed Use (Set) This technology may be deployed in the production of cancer-preventive, anti-aging, and anti-wri