A Novel Rapid Quantitative Assay for the Simultaneous Detection of Microbial Infection and Speciation

Current methods for determining whether a patient has a bacterial infection are time-consuming and may lead to delays in diagnosis and/or treatment. Real-time PCR is an emerging molecular technique, which rapidly amplifies small amounts of selected DNA regions, offering the potential for expedited identification and speciation of bacteria. Routine application of real-time PCR has been hampered, however, by technical limitations of the assay system. Using custom designed oligonucleotide primers and probes, coupled with an innovative restriction enzyme ?decontamination? step Johns Hopkins University researchers have devised a highly sensitive and specific molecular diagnostic, which allows for universal amplification and detection of bacterial DNA in human specimens. This technique allows for rapid detection, quantification and speciation of bacteria Patent (Set) US Patent No. 6,699,670

US 699,670

Inventor(s): Rothman, Richard

Type of Offer: Licensing

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