Diagnostic Patents for Sale or License

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Assays and Methods for Detecting Mycobacterial Infections

Human tuberculosis (TB), caused by bacterial pathogen Mycobacterium tuberculosis (Mtb) has been the leading cause of death by infectious diseases worldwide. TB is also the leading cause of death among people who are co-infected with HIV. It is important to accurately diagnose patients with TB infect...
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Program for Determining Progressive Hand/Foot, Regressive Hand/Foot, Neural Hands/Feet and Conflict Hands/Feet

This program makes it possible to detect hidden left-handedness in children and adults of both sexes regardless of age. The fundamental data input it uses is the number of deltas contained in fingerprints, as defined E.R Henry's system of classification (1900). One of the quirks of the centra...
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Triaxial Induction (Search-coil) Magnetic Field Sensor of an Ultimate Sensitivity for Measurement of the Environmental, Industrial, and Biological Signals

ABSTRACT- An object of the present proposal provide an improvement in search-coil type sensors. It is a further object of the present proposal to provide lightweight metallic glass antenna (pickup coil- PC) for detecting AC magnetic fields both generated by natural- and man-made sources including o...
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Compositions and Methods for Detecting Plectin-1 as a Biomarker for Cancer

The present invention provides compositions and methods useful for diagnostic and imaging techniques for detecting and localizing the biomarker Plectin-1. The present invention provides multimeric peptide ligand complexes for targeting Plectin-1, such as the multimeric peptide ligand complex having ...
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In Vivo Isotopic Labeling Method for Quantitative Glycomics

The present invention relates to a method of isotopically labeling glycans and in facilitating high throughput quantitative/comparative analysis of glycomic compositions of biological cells. The method is applicable inter alia for identifying differentiated cells and their glycomic characteristics, ...
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Diagnostic Assay for Trypanosoma Cruzi Infection

A sensitive, multicomponent diagnostic test for infection with T. cruzi, the causative agent of Chagas disease, including methods of making and methods of use. Also provided is a method for screening T. cruzi polypeptides to identify antigenic polypeptides for inclusion as components of the diagnost...
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Protein Markers Identification for Gastric Cancer Diagnosis

Methods for detecting cancer as well as methods of diagnosis of cancer by detecting proteins secreted into biological fluids are disclosed The invention was first applied to detecting proteins secreted into serum and urine However, it is understood that the methods have broader application to develo...
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Methods of Screening for Risk of Proliferative Disease and Methods for the Treatment of Proliferative Disease

A method of screening a subject for a proliferative disease risk factor comprises detecting the presence or absence of upregulation of the CLN3 gene in the subject. The upregulation of the CLN3 gene in the subject indicates the subject is at increased risk of developing a proliferative disease. Meth...
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Methods of Identifying Infectious Disease and Assays for Identifying Infectious Disease

Methods of identifying infectious disease infection prior to presentation of symptoms, assays for identifying genomic markers of infectious disease, and methods for diagnosing the underlying etiology of infectious disease....
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Diagnostic and Treatment for Chronic and Acute Phase Myeloid Leukemia

Disclosed are methods of predicting responsiveness of a cancer cell to a tyrosine kinase inhibitor, and methods of predicting the risk of progression of a cancer cell to a more aggressive form. Also provided are methods of reducing proliferation or promoting differentiation of a cancer cell having r...
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Systems and Methods for Generating an Osteoarthritis Progression Predictor and Systems and Methods for Using the Predictor

Example systems and methods of generating an indicator of osteoarthritis (OA) progression are described. Fractal dimension curves are generated for horizontal and vertical trabecular components associated with a region of interest of a joint image. A statistical model is used to model shapes of the ...
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Methods for Detecting Or Monitoring Cancer Using LPC As a Marker

A method of detecting a cancer, such as ovarian cancer, in a test subject including (a) determining the amount of a lysophosphatidyl choline in a sample of a bodily fluid taken from the test subject, and (b) comparing the amount of the lysophosphatidyl choline in the sample of the bodily fluid taken...
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Method of Predicting Non-Response to First Line Chemotherapy

The invention provides a method for determining a prognosis of colorectal cancer in a colorectal cancer patient, comprising classifying said patient as having a good prognosis or a poor prognosis using measurements of a plurality of gene products in a cell sample taken from said patient, said gene p...
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Methods of Diagnosing and Treating Cancer.

The invention relates to methods of determining an appropriate cancer therapy for a subject based on intratumoral expression levels of Ribonucleotide Reductase M1 (RRM1) and thymidylate synthase (TS) gene expression, as well as to methods of predicting clinical outcome (prognosis) based on cytoplasm...
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Materials and methods for diagnosis, prevention and/or treatment of stress disorders and conditions associated with abeta peptide aggregation

The subject invention concerns materials and methods for treating and/or preventing diseases associated with accumulation of A[beta] peptide in neural tissue. The subject invention also concerns materials and methods for treating and/or preventing stress disorders, such as post-traumatic stress diso...
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Method for Diagnosing Selected Adenocarcinomas

A method for the early diagnosing of selected adenocarcinomas in a human comprising the steps of removing a bodily sample from the human, and assaying the bodily sample for elevated expression of a specific gene. The gene being assayed for in the bodily sample is the TGFB-4 gene (hereinafter referre...
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Genotypic Tumor Progression Classifier and Predictor

Actively dividing tumors appear to progress to a life threatening condition more rapidly than slowly dividing tumors. Assessing actively dividing tumors currently involves a manual assessment of the number of mitotic cells in a histological slide prepared from the tumor and assessed by a trained pat...
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Tissue Classification Method for Diagnosis and Treatment of Tumors

The present invention discloses an informational computation method for classifying objects Specifically, the invention is a system, method, and computer-readable media for classifying tumors using a nonparametric statistical classifier in conjunction with an artificial neural network. The invention...
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Method and Assay for Early Diagnosis of Prostate Cancer

A method and assay are described for determining prostate cancer and the general stage of progression of such cancer by quantifying levels of promoter methylation of gal-3, optionally in combination with the quantification of the level of GSTP1 promoter methylation, where the method and assay are no...
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Methods and Compositions for the Diagnosis of Crohn's Disease

The present invention provides methods and materials, including kits, to evaluate Crohn's disease, including to diagnose, monitor, or determine the efficacy of treatment for Crohn's Disease. The methods involve determining the presence, absence, or level of zonulin in a subject sample. In certain em...
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Apparatus and Method for Use in Analyzing a Patient's Bowel

An apparatus and method are provided for use in studying a patient's bowel which combines recording and analysis of physiologic parameters and patient sensory perception. A pain input detector, a pain transducer, and a processor are provided. A gas pressure transducer and flow meter may also be prov...
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Methods of Diagnosis and Prognosis for a Muscular Dystrophy

The invention relates to the treatment, diagnosis, and prognosis of a muscular dystrophy or myopathy. The present inventors have found that the quantity of mu-crystallin is increased in a muscular dystrophy. In particular, the inventors have found that mu-crystallin is increased in facioscapulohumer...
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In Vivo Biofilm Infection Diagnosis and Treatment

The present invention relates to a method for in vivo detection of a biof?lm infection residing in a mammal, the method comprising (i) administering to the mammal a diagnostic-effective amount of a biofilm-specific probe, wherein the probe comprises a bio film-targeting moiety and a paramagnetic nan...
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Methods and Systems for Magnetic Focusing of Therapeutic, Diagnostic or Prophylactic Agents to Deep Targets

Systems and methods are disclosed for trapping or focusing magnetizable particles comprising therapeutic agents at a distance using a dynamic magnetic field and feedback control, to enable the treatment of diseased areas deep inside a patient's body. The methods may be used to diagnose or treat dise...
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Methods and Compositions for Diagnosing and Modulating Human Papillomavirus (HPV)

The present invention concerns methods and compositions for treating a patient having, suspected of having, or at risk of obtaining a HPV infection....
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Systems and Methods for Catheter Stability

The present invention provides systems and methods for stabilizing catheters, such as decapolar coronary sinus catheters in veins. In particular, the present invention provides fixation elements for improved tissue contact and stability....
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Perinucleolar Compartment As a Cancer Marker

The present invention relates to compositions and methods for cancer diagnostics, prognostics and predictions, including but not limited to, cancer markers. In particular, the present invention provides perinucleolar compartments and their resident molecules as cancer markers....
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METHODS FOR DIAGNOSING AND TREATING SQUAMOUS CELL CARCINOMA UTILIZING miRNA-205 AND INHIBITORS THEREOF

Disclosed are diagnostic and therapeutic methods related to squamous cell carcinoma. In particular, the diagnostic methods relate to detecting miRNA-205, thereby diagnosing an aggressive form of squamous cell carcinoma. The therapeutic methods relate to inhibiting the function of miRNA-205, thereby ...
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RNA from Cytology Samples to Diagnose Disease

The invention relates to methods and kits for detecting the likelihood that a subject has cancer, e.g., squamous cell carcinoma, by assaying the expression levels of tumor associated genes. More specifically, the expression levels of nucleic acids or proteins can be assayed in the tumor associated g...
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Apparatus and Method for Measuring a Characteristic of a Composition Reactive to a Magnetic Field

A system that incorporates teachings of the present disclosure may include, for example, a sensor having a pulse oximeter sensor to measure an oxygen saturation level in a liquid, a magnetic source coupled to the pulse oximeter sensor, and a controller to control the pulse oximeter sensor and the ma...
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Live/dead Viral Load Assay

The present invention provides viral load assays, kits and methods able to distinguish between active and inactive viruses in a sample. Current diagnostic methods for analyzing the presence of viruses in a clinical sample, or the effects of an anti-viral treatment or a microbicide can only report th...
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Detection and Prediction of Physiological Events in People with Sleep Disordered Breathing Using a Lamstar Neural Network

Apparatus and methods are disclosed for generating and outputting physiological event results from physiological data related to a patient. Physiological event results include results predicting and/or detecting individual physiological events related to a medical condition of the patient....
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Methods of Diagnosing Diastolic Dysfunction

Diagnostic methods relating to a cardiac ventricular dysfunction are provided. In some embodiments, the diagnostic method is a method of diagnosing diastolic dysfunction in the absence of systolic dysfunction in a subject. The method comprises assaying a sample obtained from the subject for evidence...
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Methods for Diagnosing Endometriosis

Provided herein is a method for diagnosing and monitoring endometriosis in a subject by measuring levels of the [beta]-subunit of fibrinogen....
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Diagnosis of Age-related Macular Degeneration Using Biomarkers

The invention relates to proteins associated with age-related macular degeneration (AMD). These proteins, which are present in blood, are expressed in individuals with AMD at either elevated or reduced levels compared to healthy individuals. The invention provides methods for diagnosing AMD. The inv...
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Diagnosis of Abdominal Aortic Aneurysm Using Biomarkers

The invention relates to proteins associated with abdominal aortic aneurysm (AAA). These proteins, which are present in blood, are expressed in individuals with AAA at either elevated or reduced levels compared to healthy individuals. The invention provides methods for diagnosing AAA. The invention ...
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Compositions and Methods for Detecting Predisposition to a Substance Use Disorder

The present invention provides screening kits, compositions, and diagnostic methods for determining whether a subject has a predisposition to, or likelihood of having, a substance use disorder by determining a nucleic acid methylation profile from a biological sample from the subject, wherein a give...
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Diagnosis and Treatment of Age-Related Macular Degeneration

The invention relates to Factor H gene polymorphisms and haplotypes associated with an elevated or a reduced risk of AMD. The invention provides methods and reagents for diagnosis and treatment of AMD....
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Diagnostics and Therapeutics for Glaucoma

The invention provides methods and compositions for diagnosing and treating glaucoma by detecting alterations in the Wnt signaling pathway-including changes in the level or bioactivity of frizzled related protein. The invention further provides methods for screening for anti-glaucomatous compounds b...
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Cell's bioactive protein production determining method, involves bonding catch antibody to beads, and analyzing spatial catch-antibody/protein/detection antibody-structures of beads for evaluating pro

The method involves bonding a catch antibody to beads e.g. Dynabeads(RTM: Superparamagnetic, monosized polymer particles). The beads are mixed with a suspension of cells in an analysis container. The beads are separated from the cell by action of a magnetic field, and brought in contact with a detec...
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A Miniaturized, Dermal-adhesive-based Device for Position-independent, Non-invasive Fetal Monitoring

A system for fetal position-independent, non-invasive fetal monitoring includes a plurality of disposable adhesive patches for placement on an expectant mother's upper and lower abdomen. Each of the patches includes one or more miniature electronic devices embedded within the adhesive patches to det...
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Guide Wire Engaging Ureteroscope

This document discusses, among other things, a ureteroscope having a notch or other structure at a distal end configured to engage a guide wire and facilitate cannulation....
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Diagnostic Test Kits

This invention provides kits, devices, and methods for the detection of antibodies that recognize one or more proteins and/or antigens from porcine reproductive and respiratory syndrome virus (PRRSV). The antibodies may be in a biological fluid of a PRRSV infected or at risk subject. The invention m...
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Spinocerebellar Ataxia Type 8 and Methods of Detection

The present invention provides an isolated nucleic acid molecule containing a repeat region of an isolated spinocerebellar ataxia type 8 (SCA8) coding sequence, the coding sequence located within the long arm of chromosome 13, and the complement of the nucleic acid molecule. Diagnostic methods based...
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Predictive and Diagnostic Methods for Cancer

The present disclosure encompasses methods of diagnosing the presence of a cancer, and particularly a cancer of prostate or breast tissue, in a human subject, predicting the outcome or severity of the disease and methods of reversing the prostate cell transformation based on the presence or absence ...
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Methods and Compositions for Improved Delivery of Therapeutic and Diagnostic Agents

Among other things, this disclosure provides for compounds having a targeting moiety linked to a therapeutic moiety and/or a diagnostic moiety; the synthetic methods for making the compounds; pharmaceutical compositions comprising the compounds; and, methods for treating or diagnosing disorders. Som...
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Methods and Systems for Screening for and Diagnosing Dna Methylation Associated with Autism Spectrum Disorders

Methods and systems for population screening and diagnostics are provided. In particular methods and systems for population screening of individuals for genetic disorders due to alterations in DNA methylation and for the diagnostic testing for such disorders are provided....
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Internet-based Cognitive Diagnostics Using Visual Paired Comparison Task

Disclosed are methods for diagnosing declarative memory loss using mouse tracking to follow the visual gaze of a subject taking a visual paired comparison test. Also disclosed are methods for diagnosing dementia such as mild cognitive impairment and Alzheimer's disease....
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Fragile X Mental Retardation Protein (FMRP), Compositions, and Methods Related Thereto

The invention relates to fragile X mental retardation protein (FMRP), compositions, and methods related thereto. In certain embodiments, the invention relates to treating a neurological disorder by administering a P13K antagonist to a subject in need thereof. In other embodiments, the invention rela...
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Methods for Applying Brain Synchronization to Epilepsy and Other Dynamical Disorders

For analyzing a multi-component system, a method acquires a plurality of signals, each having a different spatial location of the multi-component system, and generates dynamic profiles for each of the plurality of signals. Each of the plurality of dynamic profiles reflects dynamic characteristics of...
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Apparatus, System, and Method for Ultrasound Powered Neurotelemetry

The present embodiments provide an apparatus, system, and method for ultrasound powered neurotelemetry. In one embodiment, the apparatus includes a piezoelectric element configured to receive an ultrasonic pulse and convert the electronic pulse into an electric potential. A diode may be coupled to t...
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Diagnostic and Treatment Methods for Cancer Based on Immune Inhibitors

Methods of diagnosis and treatment of malignant tumors, in particular ovarian tumors, using GD3 and GD3 inhibitors. Also provided are methods of modulating the immune system of a mammal by the administration of a GD3 and GD3 inhibitors...
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Neurodegenerative Disease Diagnostic Compositions and Methods of Use

The invention generally provides diagnostics that employ biomarkers altered in neurodegenerative disease, as well as methods for the use of such markers in monitoring disease progression and identifying agents useful for the treatment of a neurodegenerative disease....
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Medical image analysis apparatus and image analysis control program

A motion function of a biological tissue is efficiently evaluated on the basis of image data collected from different medical image diagnostic apparatuses. In terms of a common analysis algorithm applied to subject's time-series image data supplied from a separate medical image diagnostic apparatus,...
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Protective Complement Proteins and Age-Related Macular Degeneration

Methods for identifying a subject at risk for developing AMD are disclosed. The methods include identifying specific protective or risk polymorphisms or genotypes from the subject's genetic material. Therapeutic compositions and methods are also provided for delaying the progression or onset of the ...
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Method for Confirming a Diagnosis of Rolandic Epilepsy

A strong association between variants in Elongator Protein Complex 4, (ELP4) (specifically single nucleotide polymorphisms, SNPs) at the 11p13 locus on chromosome 11 and the centrotemporal sharp wave trait (CTS) has been discovered, which association has diagnostic significance for rolandic epilepsy...
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Diagnosis and Therapy of Organ Dysfunction Using Sphinganine-1-phosphate

The invention relates to the treatment and diagnosis of organ dysfunction caused by ischemia reperfusion injury. In particular, the invention relates to sphinganine-1-phosphate, a sphingolipid metabolite, and its use in the diagnosing, preventing, and/or treating ischemia reperfusion-associated diso...
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Integrated Sensor Network Methods and Systems

Methods and systems for an integrated sensor network are described. In one embodiment, sensor data may be accessed from a plurality of motion sensors and a bed sensor deployed in a living unit for a first time period. An activity pattern for the first time period may be identified based on at least ...
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Immunoassay for Venom Detection Including Noninvasive Sample Collection

Methods and immunoassays for diagnosing a bite or sting of a venomous organism in a patient having symptoms consistent with such a bite or sting are provided. A sample of venom is collected from the area of the suspected bite or sting using a swab and then contacted with an antibody that specificall...
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Prediction and Diagnosis of Canine Degenerative Myelopathy.

The present invention provides for methods of identifying a dog carrying a major genetic risk factor in the SODl gene for degenerative myelopathy, a potential model for human amyeotrophic lateral sclerosis. Also provided a methods of early diagnosis, treatment and breeding based on the presence or a...
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Compositions and Methods for Early Pregnancy Diagnosis

Disclosed are antibodies and methods for detecting pregnancy in an animal. In certain aspects antibodies used binds immunologically to at least two PAGs selected from PAG4, PAG6, PAG9, PAG16, PAG17, PAG19, PAG20 and PAG21. Antibody encoding nucleic acids are also provided, as are kits, methods of us...
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Methods and Compositions for Diagnosis of Urosepsis and Urinary Tract Infection

Methods for diagnosis of, and distinguishing between, urosepsis, sepsis, and urinary tract infections (UTIs) are disclosed The diagnostic methods are based on determining the level of NGAL protein in a bodily fluid sample, such as urine sample....
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Systems, Methods and Computer-accessible Media for Hyperspectral Excitation-resolved Fluorescence Tomography

An exemplary method, system, and computer-accessible medium can be provided for generating three-dimensional image information associated with a fluorescence-exhibiting arrangement within a sample. For example, it is possible to generate a first electro-magnetic radiation, to be received in the samp...
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Devices, Systems, and Methods for Evaluating Vision and Diagnosing and Compensating Impairment of Vision

A system and method for evaluating and predicting visual perception of animals are provided. Retinal image data or other data representing the pathology of an animal is provided as input to a nonlinear computational model of a primary visual cortex of an animal. A plurality of images of a predetermi...
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Methods of Diagnosing, Preventing and Treating Bone Mass Diseases

The present invention provides methods and therapeutic agents for lowering or increasing serum serotonin levels in a patient in order to increase or decrease bone mass, respectively. In preferred embodiments, the patient is known to have, or to be at risk for, a low bone mass disease such as osteopo...
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Use of Urinary Ngal to Diagnose Sepsis in Very Low Birth Weight Infants

Methods for diagnosis of sepsis very low birth weight (VLBW) infants are disclosed. The diagnostic methods for sepsis are based on determining whether a bodily fluid sample, such as a urine sample, contains an amount of neutrophil gelatinase-associated lipocalin (NGAL) protein that exceeds or is les...
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Serum-based, Diagnostic, Biological Assay to Predict Pregnancy Disorders

The invention provides serum-based, diagnostic, biological assays for predicting disorders of pregnancy resulting from poor trophoblast and/or placental ischemia, including preeclampsia. Serum samples from such subjects exhibit an ability to disrupt the architecture involving fetal trophoblasts and ...
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Methods of Diagnosing and Treating Carcinomas

Methods of diagnosing a carcinoma include comparing the expression of miRNAs in a sample with a control and determining ratios of expression of miRNAs. Methods of treatment include administering a nucleic acid encoding a miR-375 gene product. Methods of optimizing treatment in a subject include dete...
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Diagnosing, Prognosing, and Early Detection of Cancers By Dna Methylation Profiling

A method of employing DNA methylation analysis for the diagnosis, prognosis, and prediction of cancer....
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Methods and Products for Diagnosing Cancer

The present invention relates to methods and kits for the detection and diagnosis of cancer or precancerous conditions. Methylation of specific genes has been identified as indicative of cancer and methods of the invention, in part, relate to the detection of methylation levels in cells as a determi...
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Detection of Fragments of Nectin-1 for the Diagnosis of Alzheimer's Disease

Methods for diagnosing Alzheimer's Disease by detection of fragments of nectin-1 are described. Nectin-1 is shown to be a substrate for proteases associated with the onset of Alzheimer's Disease, including [alpha]-secretase, [gamma]-secretase and BACE1 or a BACE1-like protease. The fragments produce...
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Methods for Diagnosing and Treating Pelvic Pain Disorders Via Beta-catenin

Methods for diagnosing a pelvic pain disorder that include analyzing samples for increased levels and/or nuclear accumulation of beta-catenin, which indicates presence of the pelvic pain disorder, particularly interstitial cystitis. Therapeutic treatment of a pelvic pain disorder, particularly inter...
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Simultaneous Ambulatory Pulse Oximetry and Ph Monitoring for the Diagnosis of Gerd-related Respiratory Disease

Respiratory diseases related to gastroesophageal reflux disease in a patient are diagnosed by detecting esophageal or pharyngeal impedance and pH in the patient, detecting oxygen saturation in the patient, and diagnosing the respiratory diseases from the esophageal or pharyngeal impedance, the pH, a...
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Methods of Diagnosing and Treating Fibrosis

The present invention is directed to methods of diagnosing and treating a fibrotic condition in a mammalian subject. These methods involve measuring the levels of trimethylation at lysine residue 27 of histone-3 and/or measuring the expression levels of EZH2 or YY-1. Agents useful for treating fibro...
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Compositions and Methods Relating to Detection of Soluble E-cadherin in Neurodegenerative Disease

Disclosed herein are compositions and methods for diagnosing, detecting, or monitoring neural disease, conditions, or disorders involving detection of soluble E- cadherin....
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Apparatus and Method for Cone Beam Volume Computed Tomography Mammography

Volume tomographic mammography is performed with a gantry frame (206) in which a cone-beam radiation source (210) and a thin-film detector (208) are mounted. The patient (P) rests on an ergonomically designed table (202) with a hole (204) to allow one breast (B) to extend therethrough such that the ...
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Ovarian Cancer Methylome

The present invention provides methods and kits for identifying a cell that exhibits or is predisposed to exhibiting unregulated growth by detecting hypermethylation of a gene or a regulatory region in at least one gene in the cell. Also provided are methods for diagnosis or prognosis of ovarian can...
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Recognition of Cyp2e1 Epitopes

A critical epitope of human cytochrome P4502E1 (CYP2E1) associated with the development of hepatic autoimmune disease, including methods and kits for diagnosis and prognosis using the critical epitope as a biomarker for hepatic autoimmune disease....
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Method for the Diagnosis of Age-associated Vascular Disorders

Methods for determining if a subject has or is susceptible to having an age- associated vascular disorder are disclosed. The method includes determining if the subject exhibits altered expression of a product of one or more of the genes listed in Table 1 relative to a control level of expression of ...
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Compositions and Methods for Diagnosing and Treating Inflammatory Bowel Disease and Related Disorders

The present invention features biomarkers capable of diagnosing inflammatory bowel disease and methods of using such biomarkers to diagnose and selecting treatments for inflammatory bowel diseases....
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System for Diagnosis and Therapy of Gaze Stability

A system to measure a subject's gaze stability, comprising: a display device; a data processor in communication with the display device; a data input device in communication with the data processor; and a motion detector in communication with the data processor, the motion detector being adapted to ...
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Novel Desmin Phosphorylation Sites Useful in Diagnosis and Intervention of Cardiac Disease

This invention relates to novel phosphorylation sites in the desmin protein that are associated with the onset of heart failure. The phosphorylation sites, i.e., Ser-27 and Ser-31, can be used as biomarkers for (i) identifying subjects at risk for the development of heart failure, (ii) treating subj...
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Neoepitope Detection of Disease Using Protein Arrays

A diagnostic device for and method of detecting the presence of head and neck squamous cell carcinoma (HNSCC) in a patient including a detector device for detecting a presence of at least one marker indicative of HNSCC, the detector device including a panel of markers for HNSCC. A diagnostic device ...
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Biomarkers for Diagnostic and Therapeutic Methods

Erythrocyte ATP-release modulators and composition and methods for their use as biomarkers of glucose processing or vascular disorders, as well as methods for screening to identify to modulators; methods for monitoring efficacy of therapy; and apparatus for use in such methods....
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GALECTIN-3 nsSNP MARKER FOR CANCER

A diagnostic biomarker including a mechanism for determining a patient's propensity to develop cancer. A diagnostic kit for determining a patient's propensity to develop cancer. A method of predicting both a patient's and a population's propensity to develop cancer, by detecting the presence of an H...
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Treatment and Diagnostic Methods for Fibrosis Related Disorders

The present invention relates to the ability of SAP to suppress fibrocytes. It also relates to the ability of IL-12, laminin-1, cross-linked IgG and IgG aggregates to suppress fibrocytes. Methods and compositions for suppressing fibrocytes using these proteins are provided. These methods are useful ...
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Method for Detecting Disease Markers

Disease specific markers, in particular cancer markers, can be detected by electrophoretically separating proteins and protein complexes from a biological sample on a protein binding polymeric membrane in a low conductivity, water-miscible organic solvent buffer. Electrophoretic separation profiles ...
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Automated Assessment of Atrioventricular and Ventriculoatrial Conduction

A method discriminates between ventricular arrhythmia and supraventricular arrhythmia by determining the direction of an electrical signal conducted through the atrioventricular node. An implantable cardiac defibrillator provides atrioventricular and ventriculoatrial pacing bursts to determine if an...
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Diagnostic and Therapeutic Uses of Augmenter of Liver Regeneration in Inflammatory Conditions

The present invention provides for methods and kits for detecting sepsis, trauma/hemorrhage or inflammation in a subject. It is based, at least in part, on the discovery that Augmenter of Liver Regeneration ("ALR") is an early marker of these conditions. Accordingly, in other embodiments, the presen...
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Marker Panels for Idiopathic Pulmonary Fibrosis Diagnosis and Evaluation

The present invention relates to the discovery that of a panel of serum or plasma markers may be used to diagnose Idiopathic Pulmonary Fibrosis ("IPF") and distinguish this condition from other lung ailments. It further relates to the identification of markers associated with IPF disease progression...
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Therapeutic and Diagnostic Cloned Mhc-unrestricted Receptor Specific for the Muc1 Tumor Associated Antigen

The invention provides an isolated nucleic acid encoding a receptor, other than an immunoglobulin, wherein the receptor binds to a MUC1 tumor antigen independently of an major histocompatibility complex (MHC). The invention provides a method of activating a signaling pathway and/or killing a cancer ...
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Prediction and Prevention of Preeclampsia

The present disclosure relates to methods for assessing increased risk of preeclampsia in a pregnant woman. The methods described herein employ measuring relaxin levels, and optionally measuring C-reactive protein levels in a biological sample of a pregnant woman. The disclosure further encompasses ...
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Metodo En Tiempo Real Para Detectar Trastornos Inflamatorios Agudos.

Un método para diagnosticar o monitorear lupus eritematoso sistémico (SLE) en un individuo, el método caracterizado porque comprende: (a) determinar el nivel de un componente de trayectoria del complemento C4d en un reticulocito del individuo y (b) comparar el nivel del componente de trayectoria del...
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Method for Diagnosing Renal Diseases Or Predispositions

The invention provides a method of diagnosing a disease or a predisposition to contract a disease by assaying for mutations of uromodulin (UMOD) within a test subject or patient. The presence of a mutation in the UMOD supports a diagnosis of a disease or a predisposition to contract a disease within...
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Anticancer Vaccine and Diagnostic Methods and Reagents

The invention provides a method for vaccinating a patient against a malignancy comprising introducing a protein or peptide comprising of all or an immunogenic fragment of a cyclin protein into the patient. The invention further provides a method of identifying tumor antigens....
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Methods to Diagnose and Immunize Against the Virus Causing Human Merkel Cell Carcinoma

The present invention provides isolated or substantially purified polypeptides, nucleic acids, and virus-like particles (VLPs) derived from a Merkel cell carcinoma virus (MCV), which is a newly-discovered virus. The invention further provides monoclonal antibody molecules that bind to MCV polypeptid...
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Monoclonal Antibodies for Cspg4 for the Diagnosis and Treatment of Basal Breast Carcinoma

It is disclosed herein that condroitin sulfate proteoglycan 4 (CSPG4), also known as high molecular weight melanomal associated antigen, is overexpressed on basal breast carcinoma cells (BBC), specifically triple negative basal breast carcinoma cells (TNBC). Methods for detecting basal breast cancer...
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Method for Immune Response Detection

A method for detecting one or more immunological response factors that is expressed in response to a therapeutic treatment and/or disease using a piezoelectric microcantilever sensor (PEMS) to assess a patient's immunological response. The method involves measuring a frequency shift of the PEMS caus...
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A High Precision Quantitative Assay Composition and Methods of Use Therefor

The invention features compositions and methods that are useful for precisely determining the amount of one or more analytes present in a sample. In one aspect, the invention provides a composition for measuring the abundance of one or more target analytes in a sample, where the composition contains...
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Mutations of the PIK3CA gene in human cancers

Phosphatidylinositol 3-kinases (PI3Ks) are known to be important regulators of signaling pathways. To determine whether PI3Ks are genetically altered in cancers, we analyzed the sequences of the P13K gene family and discovered that one family member, PIK3CA, is frequently mutated in cancers of the c...
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Detection of Auto-antibodies to Specific Glycans As Diagnostic Tests for Autoimmune Diseases

The invention provides uses, methods, and kits for diagnosing an autoimmune disease, particularly scleroderma and systemic lupus erythematosus, in a subject by detecting the presence of one or more antibodies that specifically bind to one or more glycans in a subject sample....
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Methods for the Diagnosis and Treatment of Lung Cancer

The present invention provides methods for aiding in the diagnoses of the neoplastic condition of a lung cell and methods of screening for a potential therapeutic agent for the reversal of the neoplastic condition....
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Method, Kit Or Diagnostic for the Detection of Reagents which Induce Altered Contractility

A method of screening for compounds that enhance or depress contractile function, based on measuring the formation of heterodimers of contractile fibers (e.g. Tm and actin, myosin heavy and myosin light chains), for example through disulfide bond formation. Diagnostic and prognostic methods and kits...
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Microrna-based Diagnostic Testing and Therapies for Inflammatory Bowel Disease and Related Diseases

The present invention is based, at least in part, on the novel discovery that certain microRNAs are associated with inflammatory bowel diseases and other related diseases. Accordingly, the invention relates to microRNA-based compositions, kits, and methods for detecting, characterizing, modulating, ...
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Sr-bi As a Predictor of Human Female Infertility and Responsiveness to Treatment

Methods of diagnosis and treatment of diseases and disorders related to de novo synthesis of cholesterol, based on allelic variants of the scavenger receptor class B type I receptor, and kits for use therein....
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Detection of Prostate Cancer Using Psa Glycosylation Patterns

The present invention features novel methods for determining if a subject has prostate cancer. The present invention is based on the development of lectin immunosorbant assays which analyze [alpha]2,6-linked sialylation of total serum PSA by sambucus nigra lectin (SNA) and [alpha]2,3-linked sialylat...
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Image-based Risk Score-a Prognostic Predictor of Survival and Outcome from Digital Histopathology

The present invention relates to an image-based computer-aided prognosis (CAP) system and method that seeks to replicate the prognostic power of molecular assays in histopathology and pathological processes, including but not limited to cancer. Using only a tissue slide samples, a mechanism for digi...
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Treatment and Diagnosis of Inflammatory Disorders

This invention concerns compositions and methods of treating or diagnosing inflammatory disorders and other disorders....
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Genes As Diagnostic Tools for Autism

The homeobox transcription factor, ENGRAILED 2 EN2), was investigated for association with autism spectrum disorder (AS) by performing transmission/disequilibrium tests (TDT) for two SNPs (rs1861972 and rs1861973) and was identified as being a likely susceptibility locus for autism and related AS di...
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Molecular Circuits

Disclosed herein are autonomous molecular circuits that can function in cells. The circuits can process logical operations in which one or more input cues are among the operands and produce an appropriate output. Such circuits can be implemented in living cells, e.g., eukaryotic or prokaryotic cells...
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Peptides and Nanoparticles for Therapeutic and Diagnostic Applications

Provided herein are peptides and nanoparticles conjugates thereof useful for the treatment of diseases and disorders mediated by GIPC/synectin, such as cancer....
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System and Method for Use of Nanoparticles in Imaging and Diagnosis

A method for diagnosing certain types of cancers provides a nanoparticle agent to be uptaken by cancer cells for diagnosis and treatment of certain cancers. A compound containing nanoparticles is directed toward a tumor site, and then a predetermined time period passes to allow the nanoparticles to ...
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Methods for diagnosing and treating neuroendocrine cancer

The present invention relates to a method for diagnosing neuroendocrine cancers via detecting the presence of N-methyl D-asparate-associated (NMDA) glutamate receptors type 1 and/or type 2. Methods for preventing and treating neuroendocrine cancers are also disclosed....
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Distributed Fault Diagnosis

A distributed diagnosis algorithm based on a multi-signal digraph model of an overall system is provided. In addition, a model enables the generation of a fault-test dependency matrix (D-matrix), which summarizes the detection capabilities of tests designed for faults associated with each node. Each...
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Methods for the Detection and Monitoring of Congestive Heart Failure

Disclosed herein are methods of detecting and/or prognosing congestive heart failure by detecting a proteolytic fragment of caspase-3 such as the p17 fragment or the p12 fragment. The congestive heart failure can be of any etiology, such as systolic or diastolic heart failure, ischemic or non-ischem...
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Device, Instrument and Process for Detecting Magnetically Labeled Analytes

The present invention is directed to a device for quantitative analysis of an analyte in a liquid sample by detecting a magnetic label, an instrument for controlling the analysis process and displaying the results and a method for performing said analysis with said device and said instrument....
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Analysis of Endogenous Fluorescence Images to Extract Morphological/organization Information About Living Samples

Methods and computer program products for analyzing tissue are provided. The tissue is exposed to light at the appropriate wavelengths for inducing fluorescence. Images of the fluorescing tissue are taken at two or more depths within the tissue. The PSD function is determined for each image at a dif...
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Methods for Diagnosis of Clostridium Diffcile and Methods and Vectors for Recombinant Toxin Expression

Cell-based methods for rapid real time assay of a presence of Clostridium difficile toxin and/or cells are provided, using an assay having a toxin-enhancing antibody and a sensitive cell line carrying Fc?R receptors, and kits for this assay. An ultrasensitive cell- based immunocytotoxicity assay for...
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Multifactorial Methods for Detecting Lung Disorders

Described herein are multifactorial methods for detecting, diagnosing or aiding in the diagnosis of lung disorders or disease, e.g., lung cancer. The methods disclosed utilize multiple diagnostic paradigms, for example, to improve diagnostic sensitivity, specificity, negative predictive value and/or...
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Supramolecular Bioconjugates

The invention relates to supramolecular bioconjugates and to methods for assembling and utilizing supramolecular bioconjugates. Supramolecular bioconjugates comprise a plurality of first nucleic acids and a plurality of mediators wherein each mediator comprises a second nucleic acid complementary to...
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Classification Techniques for Medical Diagnostics Using Optical Spectroscopy

Mathematical/statistical pattern-recognition systems and methods to distinguish between different pathologies and benign conditions (e.g., normal or cancerous tissue) given spectra measured using optical spectroscopy such as elastic-scattering spectroscopy....
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Haplotype Analysis

The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the c...
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Methods for prenatal diagnosis of chromosomal abnormalities

Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention explo...
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Method for Detecting and Quantifying Rare Mutations/polymorphisms

The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as del...
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Glutamine Endopeptidases D'espÈce Rothia Et Utilisation De Celles-ci

Disclosed are glutamine endopeptidase enzymes from Rothia sp. bacteria that are naturally associated with the oral cavity, formulations comprising the glutamine endopeptidase enzymes and the use thereof for the treatment, prevention of allergic reaction and diagnosis of gluten allergy related diseas...
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Early Diagnosis of Acute Coronary Syndrome

The invention relates to methods for the early diagnosis of Acute Coronary Syndrome....
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Diagnostic Uses of Anti-h8 Monoclonal Antibodies

The invention relates to diagnostic and therapeutic uses of antibodies or antigen- binding fragments that recognize the H8 antigen, an outer membrane protein of Neisseriae. Some aspects relate to pharmaceutical and diagnostic compositions comprising antibodies or antigen-binding fragments that recog...
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St2-based Dengue Fever Diagnostic

A cytokine receptor family member (ST2), which has membrane bound as well as soluble bound forms, is elevated in patients during acute phase of dengue fever. Moreover, secondary cases of dengue fever had even more pronounced elevation of ST2 in serum in comparison to primary cases. One possible role...
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Use of IMP3 as a Prognostic Marker for Cancer

Provided herein are methods and compositions for the prognostic evaluation of a patient suspected of having, or having, cancer by assessing the expression of IMP3 in a biological sample of a patient. Methods can be used at the time of initial diagnosis of malignant tumors to identify a group of pati...
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Espfu Nucleic Acids and Proteins and Uses Thereof

Disclosed are EspFU (EspF-like polypeptide encoded by a gene of the cryptic prophage CP-933U of enterohemorrhagic E. coli) polypeptides, fragments thereof, nucleic acids that encode EspFU polypeptides, or fragments thereof, and cells including the polypeptides, fragments, and/or nucleic acids. Also ...
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Methods for Differential Diagnosis of Melanocytic Lesions

The invention provides a method for determining whether a melanocytic lesion in a patient is malignant which comprises determining a quantity of gp100 present within a nuclear compartment in stained cells in a tissue sample from the melanocytic lesion; determining a quantity of gp100 present within ...
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Methods for a Predictive Diagnostic Test for Tamoxifen

A method for determining the likelihood that a therapy involving administration of tamoxifen to a patient afflicted with an estrogen receptor positive breast cancer will provide a therapeutic benefit to the patient which comprises determining the level of expression of epidermal growth factor recept...
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Methods and Compositions for the Detection and Treatment of Preeclampsia

Methods, kits and compounds are provided that relate to the diagnosis, treatment, and/or prevention of preeclampsia....
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Diagnosis and Treatment of Age Related Macular Degeneration

Methods, compositions and kits for diagnosis and treatment of age related macular degeneration....
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Excitatory glycine receptors and methods

The invention provides isolated N-methyl-D-aspartate type 3B (NR3B) polypeptides, functional fragments and peptides, encoding nucleic acid molecules and polynucleotides, and specific antibodies. Also provided are excitatory glycine receptors, containing either NR3B or NR3A polypeptides. Further prov...
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Diagnosis of Preeclampsia

The present invention provides methods and compositions related to the detection and/or monitoring of the levels of angiogenic factors, specifically VEGF, PlGF and sFlt-1, in urine samples obtained from pregnant women and the effects of such levels on the risk of developing complications of pregnanc...
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Method for Cell Identification and Quantification with Gold Nanoparticles Through Hydrogen Ion Reduction

The method for cell identification and quantification comprises bonding specific surface proteins of cells immobilised on the surface of the electrochemical transducer with specific antibodies conjugated with gold nanoparticles, and subsequent nanoparticle detection and quantification through hydrog...
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Method and Kit for the Diagnosis of Coagulation Disorders

The invention relates to a method for diagnosing coagulation disorders by determining dense granule deficiencies in platelets. The method can be used to detect the inorganic polyphosphate content of biological samples, particularly platelets, and to establish a correlation between the amount of inor...
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Use of the Masp-52 Protein for the Diagnosis, Treatment and Prevention of Chagas Disease

The invention relates to the use of the MASP-52 protein, from the mucin-associated surface protein (MASP) family, for the diagnosis, treatment and prevention of Chagas disease, including a method for detecting the presence of the T. cruzi parasite in individuals, a method for obtaining data that can...
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Method and Kit for Diagnosing Mantle Cell Lymphoma

The invention relates to a method and kit as useful tools for classifying a patient diagnosed with mantle cell lymphoma as indolent or conventional. The method comprises: a) providing a sample from a patient with MCL; b) defining the level of expression of at least one selected gene from the group c...
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Method for the Diagnosis of Inflammatory Bowel Disease By Detecting Volatile Organic Compounds in Exhaled Air

The present invention relates to the identification of markers for the disease conditions related to Inflammatory Bowel Disease. The uses of such markers in diagnosis of inflammatory Bowel Disease and a novel method for their identification are herein described. We were able to discriminate between ...
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Method for the Diagnosis of Asthma By Detecting Volatile Organic Compounds in Exhaled Air

The present invention relates to the identification of markers for the disease conditions related to asthma. The exhaled air of the patient is analysed with GC-MS and the amount of asthma markers m the breath is used to diagnose asthma The markers used are- carbon disulfide (CS2 ), l-penten-2-on, bu...
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Method for the Diagnosis of Cystic Fibrosis By Detecting Volatile Organic Compounds in Exhaled Air.

The present invention relates to the identification of markers for the disease conditions related to cystic fibrosis (CF) The uses of such markers in diagnosis and a novel method for their identification are herein described. We were able to discriminate between patients with cystic fibrosis and nor...
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Diagnostic and Therapeutic Tools for Diseases Altering Vascular Function

The invention relates to diagnostic and therapeutic tools and applications, particularly useful in diseases that affect vascular health and in inflammatory diseases. In particular, said diagnostic and therapeutic tools employ suitable detection or modulation of endothelial glycocalyx....
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Means and Methods for Diagnosing And/or Treating a Subject At Risk of Developing Heart Failure

The present invention relates to a method for identifying a subject at risk of developing heart failure, comprising: (a) determining the level of one or more biological markers in a biological sample of said subject; (b) comparing the level of said biological marker to a standard level of the same b...
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Method for identifying a subject at risk of developing heart failure by determining the level of galectin-3 or thrombospondin-2

The present invention relates to a method for identifying a subject at risk of developing hypertensive end organ damage, such as and in particular heart failure, comprising: a) obtaining a biological sample of said subject; b) determining the level of at least one non-myocytal marker in said sample;...
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Model System for Diagnosing Lipid Metabolism

The invention relates to a population model for the analysis of blood lipoprotein physiology in a test subject comprising: a. a submodel for the production of blood lipoproteins; b. a submodel for the lipolysis of blood lipoproteins; c. a submodel for the reabsorption of blood lipoproteins; and d. a...
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Gene signature for replicative senescence in cell cultures

The present invention relates to the field of diagnostics. Specifically, it relates to means and methods for determining the replicative senescence status of a cell culture and for assessing the suitability of a cell culture for therapeutic uses....
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Correction of Phase Error in Magnetic Induction Tomography

The invention relates to correcting signals measured in magnetic induction tomography with regard to a phase error. In the tomography system, an object (OBJ) is exposed to alternating magnetic fields by transmitter coils (SP1, SP2, SP3) located at specific transmitter positions, alternating voltage ...
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Method for Detecting a Wound Infection

he present invention relates to a method for detecting a wound infection comprising the steps of : - contacting a sample obtained from a wound with at least two substrates for at least two enzymes selected from the group consisting of lysozyme, elastase, cathepsin G and myeloperoxidase, and - detect...
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Method for monitoring collagen type II degradation in cartilage

A method for improving the diagnostic assessment of cartilage degenerative processes, and to provide means of monitoring the effects of therapeutical measures taken towards arthritic diseases in most mammals utilizes an immunoassay to detect fragments of collagen type II resulting from collagenase a...
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Detection of specific nitrated markers

Methods are described for improving the diagnostic possibilities of diseases where oxidative NO-modifications occur, for example inflammatory conditions, cancer, Parkinson's or Alzheimer's disease, and to provide means of monitoring the effects of therapeutical measures taken towards such diseases. ...
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GDF15 As a Differential Marker for Spondyloarthropathy

In vitro methods for differential diagnosis of spondyloarthropathy are disclosed which involve determining the levels of GDF15 in biological samples....
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Blood Serum Marker for Detection and Diagnosis of Alzheimer's Disease

The present invention relates to a blood marker for early detection and diagnosis of Alzheimer's disease. More specifically, it relates to an analysis of the protein glycan profile in the blood, and the identification of specific peak ratio's when compared to healthy aged matched subjects that are t...
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Remote Centre of Motion Positioner

Apparatus comprising a member (6) arranged to revolve about a remote centre of motion (O) and a base link (3) coupled to a mounting fixture (1). A first link (4) and a second link (5) are pivotably coupled to the member at respective distances (L1, L2) from the remote centre of motion (O) and are tr...
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Haplotype of Katiii Gene

The present invention relates to a diagnostic marker comprising at least one single nucleotide polymorphism (SNP) in the human KATIII gene, as well as to a method for the detection of a predisposition for a psychiatric disorder or a psychiatric disease....
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New Methods for Diagnosing Autoimmune Diseases

The present invention relates to new methods of diagnosing several autoimmune diseases. More particularly the present invention relates to methods and compositions for detecting anti-heterogeneous nuclear ribonucleoprotein autoantibodies for diagnosing these autoimmune diseases. More in particular, ...
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Novel Fluorescent Boron-substituted Dipyrromethenes and Use Thereof for Diagnosis

The invention relates to novel fluorescent compounds derived from non-fluorinated dipyrromethene-boron, to a method for preparing same and to the use thereof for the fluorescent marking of biological molecules. The invention also relates to biological molecules marked with said fluorescent compounds...
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Method and Device for Reading an Emulsion

The present invention relates to a method for reading an emulsion (3) comprising drops and a continuous phase surrounding the drops, wherein said method comprises: two-dimensional scanning of the emulsion (3), and construction of a two-dimensional image of the emulsion (3) on the basis of said scann...
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Device for Reconstructing Speech By Ultrasonically Probing the Vocal Apparatus

The invention relates to a portable device for speech recognition and/or reconstruction by ultrasonically probing the vocal apparatus, wherein the device comprises at least one ultrasonic transducer (20) for generating an ultrasonic wave and receiving a wave reflected by the vocal apparatus of the u...
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Monitoring Target Endogenous Species

An electrode comprising a conducting substrate for detecting species such as nitric oxide (NO), carbon monoxide (CO), oxygen (O2) and hydrogen (H2) and a polymer matrix formed from a first layer only or first and second layers with the second layer applied to the first. The matrix forms a permselect...
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Genetic Variation Associated with Coeliac Disease

The present invention provides a method of diagnosing coeliac disease, said method comprising analysing a sample of nucleic acid from a human subject to determine the presence or absence of one or more single nucleic polymorphisms (SNPs) in one or more human chromosomal regions selected from the gro...
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Platelet Analysis

The present invention relates to a method, including a diagnostic method, for characterising platelets. More particularly, the invention relates to methods for characterising platelets by immobilising platelets on a substrate for detection and subsequent characterisation, and to devices on which suc...
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Method for Early Detection of Cancer

The present inventors have demonstrated that circulating auto-antibodies to cancer antigens hold promise as specific and sensitive biomarkers for the early detection of cancer. The present invention thus relates to methods of detecting cancer in a sample, comprising utilising a glycopeptide bait der...
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Diagnostics in a Monoplex/multiplex Format

The present invention relates to a method of detecting and/or quantifying a target molecule from a sample obtained from a subject wherein the method comprises: (i) incubating a fusion protein or conjugate comprising a Ter binding polypeptide fused to at least one anti-target molecule or fragment the...
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Method and Apparatus

A method of providing cognitive training to a user, the method including, in a processing system, presenting a cognitive task to a user, the cognitive task requiring the user to view presented information and provide at least one input response; determining the at least one input response using an i...
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Diagnostic and Therapeutic Protocols

The present invention relates generally to the fields of diagnostic assays and therapeutic protocols with respect to viral infection....
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A Method of Providing Quantitative Information About the Lower Airways of a Lung

The present disclosure provides a method of providing quantitative information about a property of the lower airways of a lung using optical coherence tomography. The method comprises inserting a probe head of an anatomical optical coherence tomography device into the lower airways. Further, the met...
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Determinants of the Acute Sickness Response

The present invention relates to a method for predicting the severity of an acute sickness response in a subject, said method comprising detecting the presence of a polymorphic marker in the subject, wherein said polymorphic marker is located in a cytokine-encoding gene, and wherein said polymorphic...
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Method for Detection of Endometriosis

A method for use in diagnosis of endometriosis in a subject comprising detecting a cytokeratin (19) gene product in a biological sample from said subject, wherein detection of the cytokeratin (19) gene product is indicative of endometriosis. The gene product may be a nucleic acid or an amino acid se...
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Novel Compounds and their Uses in Diagnosis

A compound of formula (I) wherein, X and Y independently bind TSPO, wherein X and Y are the same or different; and L is a linker that links X to Y; or a salt or solvate thereof. For preference, X and Y may be (II) or (III). The compounds may be radiolabeled with a radioisotope. Also methods for diag...
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Nitrogen-containing Macrocyclic Conjugates As Radiopharmaceuticals

The present invention relates to compounds that are useful as metal ligands and which either contain a molecular recognition moiety or can be bound to a molecular recognition moiety and methods of making these compounds. Once the compounds that contain a molecular recognition moiety are coordinated ...
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Antigenic complex for the diagnosis and treatment of porphyromonas gingivalis infection

Disclosed is a purified multimeric complex from P. gingivalis, the complex comprising at least one domain from each of RgpA, Kgp and HagA, and having a molecular weight greater than about 300 kDa. Also disclosed are methods for obtaining the antigenic complex and its use to treat Porphyromonas gingi...
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Pupillary Assessment Method and Apparatus

A system and method for assessing the function of parts of the nervous system of a subject by measuring the pupillary responses to at least one stimulus ensemble comprising a plurality of individual stimuli; the method comprising: presenting a sequence of selected individual stimuli from the at leas...
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Assay for Detection of Adrenal Tumour

The invention relates to methods, assays and kits for detecting or monitoring adrenal tumours by detecting one or more adrenal steroid metabolites. The metabolites are selected from THS (tetrahydro-11-desoxycortisol), 5-PT (5-pregnenetriol), 5-PD (pregnenediol), PD (pregnanediol), PT (pregnanetriol)...
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Method for Diagnosing and Treating Muscular Dystrophy

The invention describes a method for identifying an individual exhibiting symptoms of, or having a propensity to develop muscular dystrophy comprising determining the level of expression in a tissue sample from the individual of one or more proteins selected from pax-7, caveolin-3 and/or fast-myosin...
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Screening Method

The present invention relates to clinical diagnosis of Alzheimer's disease or early- stage Alzheimer's disease in the live patient. In particular, the invention provides a screening method which can be used to assist with diagnosis of Alzheimer's disease in live human subjects, or to identify human ...
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System for Diagnosis and Treatment of Photovoltaic and Other Semiconductor Devices

A diagnostic and self-healing treatment system for a semiconductor device, the system provides: i) a shunt busting/blocking treatment, ii) self-healing treatment, and iii) an in-situ non-contact diagnostic determination....
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Na/K-ATPase Expression As an Indicator for the Treatment of Cancer

Methods for regulating the expression of Na/K-ATPase and uses thereof, including uses in the diagnosis/prognosis and treatment of cancer, are disclosed....
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Soluble Guanylyl Cyclase A1 and A-8r Peptide As Diagnostic Markers of and Therapeutic Targets for Prostate Cancer

The present invention provides materials and methods useful to treat various sGCal- expressing cancers. Materials include peptides which interfere with sGCal's pro-survival functions, thereby resulting in apoptosis of sGCal -expressing cells. In addition, the present invention provides screening ass...
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Fluid Sampling Device

Disclosed is a fluid sampling device comprising a plurality of inlet channels, each of which is arranged to receive an airflow from an associated direction, such that a sampling medium associated with a particular inlet channel is exposed to airflow from the associated direction. The present inve...
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Biomarker

Described are gastrointestinal cancer specific biomarkers comprising the nucleic acid sequence of the Engrailed-2 (EN2) gene or the amino acid sequence of the encoded EN2 protein. Also described are uses of the biomarkers in the treatment, diagnosis, monitoring and imaging of gastrointestinal cancer...
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Method for Aiding the Diagnosis of Biliary Atresia By Determining the Level of Adma in a Blood Sample

The invention relates to a method for aiding the diagnosis of bilial atresia in a subject, said method comprising (i) determining the level of ADMA in a blood sample from said subject, wherein said blood sample comprises a dried blood spot; (ii) comparing the level of ADMA determined in (i) to a ref...
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Marker

The invention provides a method for identifying whether a pregnant subject has an increased risk of preterm labour comprising measuring the progesterone concentration in a sample obtained from the subject, wherein a reduced progesterone concentration is indicative of an increased risk of preterm lab...
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Imaging System

A method of obtaining, in a single exposure, imaging information from an object (16) representative of more than two distinct illumination images, the method comprising the steps of generating first electromagnetic waves (14) at least some of which having spatially modulated polarisation; illuminati...
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CMyBP-C and MLC2 As Diagnostic Markers of Cardiac Injury

The invention relates to markers for acute myocardial infarction (AMI), particularly markers that may be used in the rapid and accurate diagnosis of AMI or reinfarction. A method of diagnosing cardiac injury comprising identifying an elevated concentration of cardiac myosin binding protein C (cMyBP-...
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Peptidic Conjugates Comprising Polyhistidine Sequence and Free Cysteine and their Uses in Imaging

Bioconjugates for use in imaging are disclosed include a linker sequence fused to a polypeptide capable of binding a target in a biological system. The linker sequence is designed so that it is capable of being radiolabelled, e.g. with a complex comprising a radionuclide, via a free cysteine residue...
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Phosphodiesterase 4D7 As Prostate Cancer Marker

The present invention relates to phosphodiesterase 4D7 (PDE4D7) for use as a marker for prostate cancer, and the use of PDE4D7 as a marker for diagnosing, detecting, monitoring or prognosticating prostate cancer or the progression of prostate cancer. The present invention also relates to a compositi...
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Improved Method of Determining Metabolic Function

The present invention provides a novel lactate difference imaging (LDI) technique, allowing assessment of the metabolic responses of tissue over a period of time. This approach utilises lactate change over a time period as an indicator of viable tissue, and offers benefits in the management and trea...
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Sigma Ligands and IKK / NF - KB Inhibitors for Medical Treatment

The present invention relates to agents that act in concert with sigma receptor ligands to enhance their effectiveness in the treatment of cancer and inflammatory disease. It also relates to methods whereby agents can be identified that will be of use in combination with sigma ligands in the treatme...
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Apparatus and Method for the Detection of Cells

An apparatus and method for in-vivo detection of magnetically labelled biological molecules or cells, located in a flowing medium in a human or animal body such as the blood stream (13). The apparatus provides a magnetic field to a region of interest inside the body in order to attract the magnetica...
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Diagnosis and Treatment of Cell Proliferation and Differentiation Disorders Based on the FMN2 Gene

The present invention provides methods for diagnosing cell proliferation and/or differentiation disorders, compounds and methods for treating the same and methods for identifying agents potentially useful in the treatment of cell proliferation and/or differentiation disorders....
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Image quality evaluation device and method

An image quality evaluation device is disclosed in the present invention, including: an image analyzer, adapted to analyze the parameters of an image to obtain parameter values; and an evaluation unit, adapted to evaluate the quality of an image based on parameter values in line with the predetermin...
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Nucleic Acid Amplification and Testing

Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are a...
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Biosensor for Analyte Detection

This invention relates to biosensors for detecting analyte molecules, such as nucleic acids, in a sample solution. The biosensors comprise a capture electrode which has probe molecules immobilised at its surface which specifically bind to analyte molecules. The probe molecules are separated on the s...
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Methods for Reducing False Hypoglycemia Alarm Occurrence

A system and method for reducing the number of hypoglycemic alarms presented to a user is presented. The system and methods include use of model based state estimation and variable-delayed threshold values to balance the risk of not presenting an alarm caused by an actual hypoglycemic state with the...
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Visual Perimeter Measurement System and Method

In one example of the invention a patient display is provided on which a fixation graphic or video is shown in substantially the centre of the display, and then target graphics or video are shown at different positions on the display about the centre. A test subject is positioned in front of the pat...
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Biomarkers for Schizophrenia Or Other Psychotic Disorders

The invention relates to a method of diagnosing or monitoring schizophrenia or other psychotic disorder. Schizophrenia is a psychiatric diagnosis that describes a mental disorder characterized by abnormalities in the perception or expression of reality. It most commonly manifests as auditory hall...
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SCD Fingerprints

This invention relates to methods of testing, diagnosing, monitoring, prognostically stratifying and classifying disease, disorders and other medical conditions and physiological states through the detection and analysis of soluble CD antigens in a body fluid sample....
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Apparatus for variably cycling the depth of water in a water tank

Apparatus for variably cycling the depth of water in a water tank (4, 4A) between an upper water level and a lower water level. The apparatus comprises a water outlet aperture (5,5A) for location in the tank and having a size to enable water to flow out of the tank at an output rate; pulley strings ...
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Measuring amt. of contamination in soft solder bath - by converting sonic pulses produced in metal probe dipped into bath into electrical pulses and plotting curve of phase summation against time

Method of quantitatively and qualitatively determining the degree of metal impurity in a soft soldering bath consists of monitoring the sonic impulses emitted as an intermetallic phase grows on the surface of a solderable metal (2) which is in contact with the bath (6.1). The sonic impulses are tran...
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Increasing measuring precision for FMCW radar - uses auxiliary signal to mix again with differential mixing signal from transmit and reception signals

The measuring precision of an FMCW radar for location of reflection in optical fibres (LWL) is increased by forming differential frequency of a mixing signal (fM) from transmit and reception signal. The mixed signal is mixed again with a triggered auxiliary signal (fH) with tunable frequency. One of...
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Safety Method for Heating Devices and Safety Device for Performing Said Method

The method involves monitoring operation of a heating device (11) such that the heating device is deactivated by a control device (14) of a safety device by deviation of the operation from operation defaults. Room temperature of a sauna cabin (10) is detected by a temperature detection element (13) ...
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Method for testing a protective device and correspondingly equipped protective device and testing device

The method involves testing an operating procedure of a protection device (1), where functions of the procedure requires a preset operation time period during utilization of the device for protecting a power transmission network (2). A time measuring device (7) of the protection device is operated f...
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Laser Imaging System to Assess the Vitality of Pulpal Chamber of Teeth

Brief Description Researchers have developed a laser imaging system that accurately assesses the pulp vitality of a tooth. This system can assess and image the pulp vitality without pain to the patient and the method used by the system is non-invasive. Advantages Pulp vitality test is crucial ...
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Label-Free Protein Detection

Background Conventional methods to detect the presence or quantity of specific proteins or other molecules in biological samples rely on the use of target-specific probes such as antibodies, which can be detected via chemical tags or fluorescent labels. However, conjugating labels to proteins can ...
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DNA Binding Compounds for Genetic Regulation and Medical Diagnosis and Therapy

Compounds that bind to DNA could be used to target drugs to DNA and to regulate expression of genes. Indeed, many compounds have been discovered (e.g. Distamycin) which bind to the minor groove of DNA, but the bond is too weak for reliable medical application. Scientists at the University of Cal...
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Inexpensive, Sensitive, Rapid and Selective Detection of GHB (Gamma-Hydroxybutyrate)

Researchers at the University of California have invented a rapid, selective, sensitive, and inexpensive enzyme-based colorimetric assay for GHB that will provide means by which: * People can protect themselves from unknowingly ingesting GHB; * Law enforcement can obtain probable cause to detain...
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Use of Vanadium Bromoperoxidase as a Signal-Generating Enzyme for Chemiluminescent Systems: Test Kits and Analytical Methods

PATENT ABSTRACT: Aqueous compositions, test kits and methods can be used to detect hydrogen peroxide or vanadium bromoperoxidase by generating a chemiluminescent signal in the presence of the analyte. Signal generation as well as reaction kinetics are improved by using a composition which compris...
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A Method for in Vivo Visualization of Mutated Mouse Cells

BACKGROUND: One method of studying tumors in mice is by using the CRE recombination system to delete or overexpress cancer-control genes in particular tissues at particular times. However, a hurdle in studying tumorogenesis is the difficulty in monitoring the progress of tumors in vivo. Current tech...
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Hybrid Energy Transfer for Nucleic Acid Detection

BACKGROUND: The need for efficient assays to detect DNA sequences is enormous and constantly growing. The recent increases in the number of sequenced genomes for bacterial and viral pathogens as well as humans provide the potential for improved medicine and diagnostics. Molecular beacon-based nuclei...
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A Combined Gene-Based Test for Guiding Therapy in Colon Cancer

This method employs two separate molecular screening tests: one that tests for mutation in the BRAF gene sequence (reported in colon cancer), and one that tests for microsatellite instability (MSI) among the numerous adjacent DNA base pairs that repeat in tandem up to five times. These screening te...
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A prognostic test for B-cell chronic lymphocytic leukemia

Background Chronic lymphocytic leukemia (CLL) is the most common type of adult leukemia in the Western world. The American Cancer Society estimated 15,340 new cases of CLL and 4500 deaths from the disease in the US in 2007. Most cases of CLL affect the B-cells, and this form of the dise...
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Antibodies for Amyotrophic Lateral Sclerosis

The Background Amyotrophic lateral sclerosis (ALS) is an adult-onset neurodegenerative disease affecting primarily motor neurons of the motor cortex, brainstem and spinal cord. Pathological hallmarks of ALS are protein inclusion bodies found in the diseased motor neurons. Disease starts as a mil...
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Method for Determining Peripheral Breast Thickness

Background Each year, 182,000 women are struck by breast cancer in the United States alone. The earlier the disease is detected, the better the prognosis. To this end, there are about 14,000 mammography machines installed in the United States, performing about 24 million mammograms a year, nine...
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An Optical Method to Measure the Thickness of Compressed Objects

Background Each year, 182,000 women are struck by breast cancer in the United States alone. The earlier the disease is detected, the better the prognosis. To this end, there are about 14,000 mammography machines installed in the United States, performing about 24 million mammograms a year, nine...
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Low-cost Flexible Manufacturing Techniques for Bradbury-Neilson Gates and Similar Interdigital Structures for Use in Miniature Time-of-Flight Mass Spectrometers and Other Related Applications

Small devices that can rapidly and accurately analyze chemical and biological molecules are required by many industries. Homeland security and the medical community lead the list. Current instruments are either too large to be used as a first responder in the field, too slow in giving results to ana...
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Molecularly Imprinted Polymer Sensor for 2-Phosphonobutane 1,2,4 Tricarboxylic Acid

Cooling towers, which are an inexpensive and environmentally friendly method to control process and building temperatures, require the addition of anti-fouling and corrosion inhibiting chemicals. PBTC (2-phosphonobutane 1,2,4 tricarboxylic acid) and HEDP (hydroxy ethylidene diphosphonic acid) are th...
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Flash Hit Detector

A sensor array system for detecting the position of an object or energy source. The system includes a plurality of sensors, each sensor having a limited field of view and being capable of detecting an object or energy source that is positioned within its field of view. The fields of view of at least...
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Method and System for Detecting Small Structures in Images

The potential difficulties and pitfalls of many automated detection techniques can be summarized as follows: a. Too little enhancement may preclude the detection of minor microcalcification peaks while too much enhancement may increase significantly the amplitude of small background structures (nois...
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Fiber Optic Ionizing Radiation Detection

An ionizing radiation detector employs optical fibers as the medium for sensing ionizing radiation emitted by a radioactive source. Light in the infrared region is pumped continuously through an optical fiber located in an area or region where the unintentional discharge of ionizing radiation may be...
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Capacitive Chemical Sensor Using an Ion Exchange Surface

A capacitive chemical sensor is disclosed that uses an ion exchange layer to detect analyte ions in a liquid medium. An exchange mechanism occurs on the surface of the ion exchange layer, wherein portions of the counter-ions are removed from the surface in favor of analyte ions. The resulting moveme...
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Fluorine Reporter Groups Easily Attached to Biomolecules for MR Imaging

Abstract (Set) Accordingly, the present invention is directed to biological compounds derivatized so as to contain at least one perfluoro-t-butyl moiety for use in fluorine-19 NMR and/or MRI studies. The perfluoro-t-butyl (PFTB) moiety, ##STR1## is an excellent reporter group for fluorine-19 NMR/MR...
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Monoclonal Antibody

Abstract (Set) A rat hybridoma producing a high-affinity IgG2a monoclonal antibody (B3B4) directed against the murine lymphocyte IgE receptor (FcR) was established by using purified FcR from FcR+ murine hybridoma B cells as immunogen. The monoclonal and polyclonal anti-FcR inhibited the binging of ...
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CF Mutations in the CFTR Gene

Mutations were found in the cystic fibrosis conductance regulator protein (CFTR) between codons 548 - 560. These mutations are associated with the cystic fibrosis phenotype and provide a genetic screen or diagnosis of cystic fibrosis....
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Anti-viral Target for Herpes Virus

Abstract (Set) A herpes virus proteinase has been identified and cloned from cytomegalovirus. The substrate for the proteinase, which is an assembly protein precursor, has also been identified. The proteinase is useful in an assay for identifying inhibitors as potential antiviral therapies. Such in...
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Exemer Ablation Within Bowman

Abstract (Set) Method and apparatus for laser ablation of the cornea to correct strabismus. The laser beam is controlled by a system of limiting shutters to cut prism lenses into the cornea. The ablation is to remain substantially within the Bowman's layer, keeping scarring to a minimum....
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Occlusion of Subretinal Vascular Beds by Laser Targeted Photodynamic Therapy

The methods and materials in this invention relate to selective and non-invasive visualization, or treatment such as chemical occlusion, of vasculature in the mammalian eye. The methods utilize fluorescent dyes and tissue-reactive substances encapsulated within heat-sensitive liposomes which are sub...
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Occlusion of Subretinal Vascular Beds by Laser Targeted Photodynamic Therapy

The methods and materials in this invention relate to selective and non-invasive visualization, or treatment such as chemical occlusion, of vasculature in the mammalian eye. The methods utilize fluorescent dyes and tissue-reactive substances encapsulated within heat-sensitive liposomes which are sub...
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Multivariate Statistical Modeling Biomarkers as Predictors of Recurrence Following Radical Prostatectomy

A method for screening individuals at risk for prostate cancer progression is disclosed. The method is useful for evaluating cells from patients at risk for recurrence of prostate cancer following surgery for prostate cancer. Specifically, the method uses specific Markovian nuclear texture factors, ...
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A New Tumor Suppressor Gene

A new tumor suppressor gene, HIC-1, has been found. Abnormal DNA methylation of a CpG island, which includes this gene, was found in breast, brain, renal, lung, colon cancer and leukemias. In low-grade astrocytomas, 100% of tumors showed abnormal methylation of the CpG island in this new gene. 75% o...
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Staph-Enterotoxin B Receptor

Abstract (Set) We have discovered that a glycoshphingolipid ,digalactosylceramide serves as a receptor for SEB.And we have developed a recptor based immunoassay to detect n mole level of this toxin in blood Description (Set) Proposed Use (Set) This technology may be used in detecting small qu...
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A Novel Mutation Involved in Obesity and Type II Diabetes

A mutation has been found in the Beta3-adrenergic receptor gene, which is associated with obesity and non-insulin dependent diabetes mellitus (type II). The presence of the mutation indicates a susceptibility to obesity and earlier onset of diabetes. The initial genotyping was carried out in the Pim...
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A Formulation of Anatomical Relationships in the Coronary Distribution of Non-calcified Plaque Based on Multi-detector CT Angiographic Assessment

Abstract (Set) Analyzing the data from coronary imaging of plaque is a complex process that makes it extremely difficult to compare any coronary plaque related information in a population-based study. The method disclosed here presents a system of statistical and analytical techniques to identify s...
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New Method of Collecting Exhaled Breath Biomarkers at Room Temperature

Abstract (Set) A simple method of collecting biomarkers that is fast, simple, and accurate is important for both research investigations and clinical management. There is currently limited number of methods to obtain breath biomarkers noninvasively. This new method of biomarker collection though ex...
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DNA Methylation for Diagnosic and Prognostic Determination in Prostate Cancer

Abstract (Set) Prostate cancer is the most commonly diagnosed cancer affecting men in the United States. Biochemical (PSA) recurrence of prostate cancer following radical prostatectomy remains a major problem. Better biomarkers are needed to identify high and low-risk patients. DNA methylation of p...
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Alpha-Tocopherol Transport Protein: Compositions and Methods ( 95014)

Researchers at Northwestern have isolated the complete coding region of the human alpha-tocopherol transport protein....
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A System and Method to Manage the Diagnosis and Therapy of Recurrent Urinary Tract Infections

Urinary tract infections (UTIs) account for more than 7 million outpatient visits to physicians' offices and well over one million hospital admissions in the United States annually. In ambulatory patients alone, the national health care cost of UTIs is estimated to approach $1 billion. In an era in ...
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Selective, Facile, Quantitative Detection of Oxidized Abasic Lesions in DNA

Abstract (Set) A variety of structural modifications (lesions) are produced in DNA when the biopolymer is exposed to oxidative stress. Lesions can be genotoxic and/or cytotoxic, and are useful biomarkers. Selective, quantitative detection of lesions is useful for rapidly determining the effects of ...
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Serial Assessment of Human Tumor Burdens in Mice by the Analysis of Circulating DNA

Abstract (Set) It is generally agreed that internal human xenografts provide the best animal models to study the microenvironments and metastatic processes occurring in human cancers. However, the use of such models is hampered by the logistical difficulties of reproducibly and simply assessing tum...
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Biomarkers Differentially Expressed in Ovarian Cancer Cells with Deactivation of the MAPK Pathway

Abstract (Set) Activation of the MAPK signaling pathway has been implicated in many human cancers. Scientists at JHU have discovered that the expression profile of two biomarkers in the ovarian cancer cell line MPSC1 is regulated by the deactivation of the MAPK pathway. Through real-time PCR, they ...
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Methods and Use of Labeling Cells with X-ray and Ultrasound-visible Agents

Abstract (Set) Cell tracking has found major applications in immune and stern cell therapy. This is commonly done with MRI and MR contrast agents, radionuclide imaging agents, or positron emission tomography agents. No methods exist for efficient radiopaque labeling and X-ray tracking of cells, nor...
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Head & Neck Squamous Cell Carcinoma an Non-Small Cell Lung Cancer Prognostics and Diagnostics

Abstract (Set) Epigenetic changes have been implicated in the pathogenesis of solid tumors and almost all types of cancers primarily via abnormal promoter hypermethylation and silencing of tumor suppressor gene expression. Epigenetic changes are changes in an organism brought about by alterations i...
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Enzyme to Specifically Cleave Lys48-linked Polyubiquitin

Abstract (Set) A member of the deubiquitinating enzyme (DUB) family has been shown to associate with the ubiquitin E3 ligase GRAIL and to control GRAIL expression. Johns Hopkins researchers now show that the same DUB is a highly specific cleavage enzyme, cleaving only lysine 48 (K48)-linked polyubi...
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Film Immobilized Capture-Particles

INVENTION: A new method of performing and analyzing protein-protein interactions, immuno-hybridization, and nucleic hybridization has been developed. This novel method utilizes capture particles immobilized on a thin film coated on a planar substrate. The particles can be arranged on the substrate a...
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Use of a Cellular Compartment as a Prognostic Marker of Breast Cancer

INVENTION: A cellular compartment (termed Cancer Compartment 1 or CC1) that is present predominantly in transformed cells and correlates with the degree of malignancy of breast cancer has been identified (see below). CC1 can be identified in tissue staining or using ELISA and flow cytometry. The cor...
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A Uniform Analysis Platform for Detection of Multiple Types of Cancer

INVENTION: A diagnostic tool capable of detecting multiple cancer types early in development from a single sample of blood. SIGNIFICANCE: The ability to detect and diagnose cancer early in its development is critical to the successful outcome of treatment. While new therapies steadily improve out...
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Biomarkers for Chronic Pelvic Pain Syndrome

INVENTION: Northwestern researchers identified MIP-1a and MCP-1 as biomarkers for Chronic Pelvic Pain Syndrome (CPPS). These markers are easily assayed from expressed prostatic secretions. These are the first biomarkers that are able to diagnose both inflammatory (Type IIIa) and non-inflammatory (Ty...
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Self-immolative Magnetic Resonance Imaging Contrast Agents Sensitive to beta-glucuronidase

INVENTION: Northwestern researchers have created a Magnetic Resonance Imaging contrast agent providing a non-invasive diagnostic for necrotic tumors. The contrast agent is highly sensitive to the enzyme beta-glucuronidase, present in high extracellular levels near necrotic tumors due to an immune re...
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Biomarkers for Pancreatic and Biliary Cancers

Abstract (Set) Pancreatic and biliary cancers are two of the most fatal among all human malignancies. Late presentation of the disease and lack of diagnostic markers are the major reasons for a dismal outcome of these cancers. The most widely used diagnostic marker for biliary and pancreatic cancer...
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Detection of Antibodies to Specific Glycans as a Novel Diagnostic Test for Autoimmune Disease

Abstract (Set) JHU scientists have identified biomarkers specific for autoimmune disease. Systemic autoimmune diseases are life- threatening, complex and can involve various organ systems including skin, joints, the renal system, the hematopoietic system, and the cardiovascular system. In autoimmun...
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Genomewide Analysis of Chromosomal Translocation

Abstract (Set) The cancer genome is quite unstable with many aberrant genetic translocations dispersed across its sequence. Many chromosomal translocations result in chimeric transcripts resulting in elevated expression levels, believed to be responsible for tumorigenesis. Therefore, it is importan...
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The Human C3b/C4b Receptor (CR1)

The present invention relates to the C3b/C4b receptor (CR1) gene and its encoded protein. The invention also relates to CR1 nucleic acid sequences and fragments thereof comprising 70 nucleotides and their encoded peptides or proteins comprising 24 amino acids. The expression of the CR1 protein and f...
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MicroRNA-based Diagnostic Testing and Therapies for Inflammatory Bowel Disease

Ulcerative colitis (UC) and Crohn's disease (CD) are the two most common chronic inflammatory bowel diseases (IBD). They often share similar clinical characteristics, such as acute and chronic inflammation of the colon, which makes accurate diagnosis difficult. Studies examining the global gene expr...
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Identification of a Novel Vertebrate-specific chaperone, BBS12, That Causes Bardet-Biedl Syndrome

Bardet-Biedl syndrome (BBS) is an autosomal recessive disorder that occurs in approximately 1in 150,000 individuals. The syndrome is characterized by retinitis pigmentosa, polydactyly, obesity, mental retardation, hypogenitalism, renal dysplasia, and short stature. Scientists from JHU, Baylor and Fr...
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Novel Drugs That Can Induce EBV Lytic Infection as a Potential Therapy for EBV Associated Tumor or to Assist in Diagnostic Imaging

Epstein Barr Virus (EBV) is a human gamma herpes virus that can establish either a latent or lytic infection in host cells. In its latent form, EBV is associated with a number of malignancies such as Burkett?s lymphoma, nasopharyngeal and gastric carcinomas and B-cell lymphoproliferative tumors. EBV...
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Diagnostic Assays for a Polymorphism in a Gene That Affects Cardiovascular Health

High density lipoproteins (HDL) are considered good or healthy cholesterol that can protect against heart disease. However, there are people who have health problems despite having desirable levels of HDL. There are a number of factors that can contribute to the blood levels of HDL, including geneti...
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Near-infrared Fluorescent 6-0-glucosamine Compounds with Targeting Specificity to Lysosomes in Tumors

Optical imaging is a non-invasive, diagnostic imaging technology which can be used to inexpensively image pathological conditions with high sensitivity and low tissue irradiation. Technologies using near infrared (NIR) light are preferred in molecular imaging studies because its long wavelength cau...
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Alternative Splice Variant Patterns of Human Telomerase Reverse Transcriptase (hTERT) in Thyroid Tumors Distinguish Benign from Malignant

Human telomerase reverse transcriptase (hTERT) is activated in thyroid cancer. Despite this, its usefulness as a marker in the distinction of benign from malignant neoplasm has not been proven. hTERT is known to have several deletion isoforms or alternative splice variants in addition to its full le...
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Method to Predict Severity of Inflammatory Central Nervous System Attacks by Detection of Interleukin-6 Levels

The present invention relates to soluble immune derangements in the cerebrospinal fluid of patients with autoimmune, inflammatory disorders of the central nervous system (CNS). Specifically, IL-6 is selectively and dramatically elevated in the CSF of these patients. IL-6 levels are directly correla...
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ATR-Seckel Cancer Cells for Drug Discovery

Advanced gene targeting methods were used to generate biallelic knock-in point mutations in human colorectal cancer cells that cause a loss of ATR function, but retain enough activity to permit viability. JHU researchers determined that progression of the cancer cell cycle after radiation treatment ...
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Enhanced Novel Diagnostic and Prognostic Markers and Therapeutic Targets for Melanoma

Presently there are few effective systemic therapies to treat advanced stages of melanoma and the key to improved survival in all affected individuals remains early diagnosis and treatment. Scientists at JHU have identified melanoma progression and invasion-associated markers that are likely to pred...
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Diagnostic Screening for Disorders of the ER-to-Golgi Trafficking

This technology describes Cranio-lenticulo-sutural dysplasia (CLSD) as a novel autosomal recessive syndrome, and identifies a missense mutation in SEC23A segregating with this syndrome. SEC23A is an essential component of the COPII-coated vesicles that transport secretory proteins from the endoplas...
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Fabrication and Application of a Prokaryotic Proteome Chip

from E. coli, and the use of the purified proteins in the fabrication of a proteomic chip. This invention also relates to various methods of using the proteomic chip to identify protein factors which mediate pathogen-host interactions, and to identify novel enzymatic activities associated with such ...
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HEYL, a Novel Oncogene

TGF-beta signal transduction is a primary tumor suppression pathway in breast cancer. Many breast cancer cells gain mechanisms to counteract the TGF-beta inhibitory effect but the mechanisms have remained elusive. Recently, scientists at Johns Hopkins University have identified a new oncogene that a...
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Mycobacterial Catalase-peroxidase in the Diagnosis and Treatment of Sarcoidosis

Sarcoidosis is an organ-wide inflammatory disease of unknown etiology that is characterized by the presence of granulomas, and frequently, pulmonary dysfunction. Currently there are no definitive diagnostic tests, and treatment regimes consist of drugs with significant side-effects. Johns Hopkins sc...
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Chest Pain Differentiation Serum Diagnostic Panel (CPDP)

Abstract (Set) Patients presenting at an emergency room with acute chest pain are likely to be evaluated for acute coronary syndrome (ACS), pulmonary embolism (PE), aortic dissection (AD) and other less fatal conditions, all of which have indistinguishable symptoms. Although rapid diagnosis and app...
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Identification of the Most Common Gene for Bardet-Biedl Syndrome

Bardet-Biedl syndrome (BBS) is characterized by multiple clinical phenotypes including retinal dystrophy, polydactyly, cognitive defects and retinal dysplasia. To date, nine BBS genes have been cloned and determined to contain 40-50% of the total mutational load of the disease. Scientists at Johns H...
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Increasing the Depth and Breadth of Coverage of Sequences on a Microarray

Microarrays are used as an investigative tool for answering questions concerning DNA sequence and structure, gene expression, and the location of protein binding targets. Although microarray systems are considered high-throughput compared to pre-existing technologies, they are currently limited by f...
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COMPARE-MS: a Novel Technique for Rapid, Sensitive, and Accurate Detection of DNA Methylation

Hypermethylation of CpG island (CGI) sequences is a nearly universal somatic genome alteration in cancer. The rapid and sensitive detection of DNA hypermethylation can aid in cancer diagnosis and risk stratification but its detection has remained labor-intensive and time-consuming. To address these ...
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HIMF and related proteins in pulmonary, cardiac and inflammatory disorders

Hypoxia-Induced Mitogenic Factor (HIMF; also known as FIZZ1/RELM? a member of the resistin protein family, is a secreted protein that was found to be highly up regulated in the lungs as a result of hypoxia-induced pulmonary hypertension. HIMF has pro-mitogenic, antiogenic and inflammatory propertie...
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Genomic and Proteomic Biomarkers for Cystic Fibrosis

Cystic fibrosis is a common lethal genetic disease which is manifest by intrinsic hyperactivation of proinflammatory signaling pathways in the lung. However, the mechanism linking the CFTR mutation to inflammatory disease is still not known. Researchers from Johns Hopkins and The Uniformed Services ...
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Novel Imaging Agent to Detect Multidrug Resistance in Tumors Using Multimodality Imaging

Multidrug resistance (MDR) can develop during chemotherapy to desensitize tumors to a variety of structurally and functionally different chemotherapeutic agents and is linked to the expression of ATP drug efflux transporters such as MDR1 P-glycoporin and MRP. MDR can be detected in vitro with molecu...
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An Algorithm for the Discovery of Robust Protein Biomarkers with Application to the Design of Classification Protein Arrays

The application of high throughput proteomics to the discovery of protein expression patterns enabling accurate, sensitive and specific prediction of disease class and progression holds great promise. Typically, tissue samples are obtained from populations of patients sharing a specific disease diag...
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Fluorescent 6-O-glucosamine compounds to label lysosomes in tumors in vivo and in cell culture systems for detection with optical imaging or fluorescence microscopy

Lysosomes contain numerous proteases, the release of which may play a vital role in breast cancer invasion and metastasis. Lysosomal trafficking may be an important factor affecting lysosomal enzyme release and effective techniques are needed to fluorescently label lysosomes and study the role of l...
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Imaging Methods for Assessment and Quantification of in vivo Antigen Capture and Vaccination

Abstract (Set) Immunization has eradicated many harmful infectious diseases in the Western world. This is commonly achieved by injecting individuals with attenuated pathogens such as viruses and bacteria. By using cancer vaccines, similar success will be achieved for eradicating the most common for...
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A Multifunctional Intermediate for Imaging PSMA

The present invention includes a novel Prostate Specific Membrane Antigen (PSMA) intermediate. This molecule can be functionalized to become either a nuclear, optical, or magnetic resonance based imaging agent as well as radiohalogen or radiometal therapeutic agents. The PSMA imaging agent can be ad...
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Inversion Recovery with On-Resonant Water Suppression

JHU researchers have developed a new technology entitled, "IRON", which stands for Inversion Recovery ON-resonant water suppression. IRON is a magnetic resonance imaging (MRI) method that is designed to image magnetic susceptibility gradients primarily associated with non-diamagnetic objects such as...
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New Class of Reporter Genes for MRI Based on Chemical Exchange Saturation Transfer (CEST)

Among the clinically applied 3D imaging modalities, magnetic resonance (MR) imaging has the highest resolution and is therefore the preferred method for detection of cellular gene expression. However, because of the low sensitivity of MR, no suitable reporter genes exist. This invention comprises a ...
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Malaria Diagnosis in Urine

Scientists at JHU have developed a urine test for malaria, the first of its kind. The test will detect aldolase and pfhistidine-rich protein using an immunoassay design. The infectious agent, Plasmodium falciparum, may also be detected using PCR methodologies. Description (Set) Proposed Use (Set...
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A High Throughput Screen Utilizing Newly Discovered Intramolecular Neuronal Calcium Channel interactions to Discover New Analgesics

This invention incorporates a newly discovered peptide which inhibits neural N-type calcium channels and the specific inter-molecular interaction that mediates the inhibitory effect of the peptide. This prototype peptide promises a beneficial profile for producing analgesia akin to opiates like morp...
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Novel Diagnostic and Prognostic Markers, and Therapeutic Targets for Melanoma

The incidence of melanoma is increasing at the highest rate for any form of cancer in the United States and the current lifetime risk in the US is 1 in 68. Presently there are few effective systemic therapies to treat advanced stages of melanoma and the key to improved survival in all affected indi...
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Diagnostic Tool for Diagnosing Benign Versus Malignant Thyroid Lesions

Thyroid cancer represents 1% of all malignant diseases and it is estimated that 5-10% of the population will develop a clinically significant thyroid nodule during their lifetime. The best available test for thyroid nodule is fine needle aspiration biopsy (FNA). The diagnosis of malignant thyroid tu...
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Microarray Gene Expression Profiling of IBD from Endoscopic Biopsy Tissues

This invention relates to the identification of biomarkers for ulcerative colitis (UC) and Crohn's disease (CD) and methods of screening. UC and CD are two highly prevalent inflammatory bowel diseases (IBDs) of the Western World. Almost all IBD patients require lifelong treatments and frequently nec...
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Nanowire Transport, Assembly, and Rotation in Suspension by AC Electric Fields

A variety of low dimensionality entities, such as nanospheres, nanodisks, nanowires, and nanotubes, have recently been explored due to their unique attributes and capabilities to bind chemical and biological targets. Nanowires posses a large aspect ratio and geometrical shape, and multi-component na...
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Transgenic Frog Lines to Study Retina Degeneration

A JHU researcher has developed lines of transgenic frogs in which transgenes induce photoreceptor degeneration in vivo and can visibly reveal the viability status of the photoreceptors through a fluorescent reporter. These frog lines produce thousands of progeny that can be used to screen therapeuti...
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PACE 4 Antisera and Cells

Rabbit antibodies against the proprotein processing enzyme PACE4; cell lines stably secreting PACE4...
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Method for the Detection of Schistosome Parasites

Schistosomes cause serious disease in humans and warm-blooded animals by living parasitically in the blood vessels draining the gut and bladder where they produce eggs that are passed out in the urine and feces. In man and animal hosts (dogs, cats, livestock), infection can produce severe debilitati...
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Monitoring Functional Kinetics of Vesicular Trafficking - Evidence for "Sleeping" Membrane Receptors on Cell Surface

The present inventive method is designed to measure the rate of protein trafficking to cell surface. Such measurement may be used to determine the physiological state of cells under different conditions including but not limited to pathological states, drug treatments, and genetic modifications. D...
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Mutational analysis of the tyrosine phosphatome in colorectal cancers

JHU researchers have performed a comprehensive mutational analysis of the protein tyrosine phosphatases super gene family in colorectal tumors and other cancers. It was discovered that the mutated tyrosine phosphatases are tumor suppressor genes, regulating pathways that may be amendable to therapeu...
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Immune Modulation Through Targeting of the MINOR Gene

this gene will induce apoptosis in dendritic cells, thereby limiting immune responses. Inhibition of the gene will prevent dendritic cell death, thereby enhancing immune responses. Dendritic cells are critical to the initiation of the immune response, thus this will provide a central tool to manipul...
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Method and Kit for Diagnosis and Drug Screening Towards Psychiatric Disorders

This invention relates to a method and kit for diagnosis and drug screening towards psychiatric disorders. In particular, it provides as an example the utilization of the known molecular diversity of Disrupted-in-Schizophrenia 1 (DISC1), a candidate gene for Schizophrenia, for objective diagnosis, o...
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Diagnostic and Prognostic Markers and Treatment Strategies for Multiple Sclerosis

To date, there are no definitive biomarkers to assist clinicians in the diagnosis of multiple sclerosis (MS) nor are there tools that accurately prodict the future clinical course of disease or the response to treatment among individul patients. Recently, a method that uses chromotographic surfaces ...
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A Method to Convert Single Base Changes to Large DNA Differences for Accurate and Sensitive Detection

In cancer and other human diseases, single-base mutations have an important role and can be used as markers for diagnostic tests. Unfortunately, in clinical samples small numbers of mutant cells are often mixed with a vast excess of wild-type cells. Therefore, that is very important to develop sensi...
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A Transgenic Mouse Line Expressing Green Fluorescent Protein in Cells Containing Endogenous Notch Pathway Activation

The TNR1 mouse line (Transgenic Notch Reporter) contains exogenous DNA stably integrated into the genome. The transgenic sequence includes binding sites for a DNA binding protein CBF1 (also known as RBP-J) upstream of a basal SV40 promoter and a cDNA encoding the enhanced green fluorescent protein ...
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Autonomous Ingestible Probe for Diagnosis and Therapy of Gastrointestinal Lesions Using Fluorescent Molecules

The invention is a Fluoropill (FP) - a swallowable pill-sized capsule camera for internal viewing of the digestive system and for transmitting video data to a reception system located outside the patient's body. The capsule camera is designed to detect diseases of the gastrointestinal (GI) tract, su...
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Registration of Ultrasound to Fluoroscopy (RUF)

The invention pertains to low dose-rate brachytherapy of early stage prostate cancer. It provides intra-operative fusion of ultrasound and X-ray fluoroscopy imagery, thus allows for real-time optimization of the implant. Description (Set) Proposed Use (Set) Transrectal ultrasound guided transp...
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Widespread Requirement for Ligand-stimulated Hedgehog Pathway Activity in Growth of Digestive Tract Tumors

This work demonstrates that hedgehog (Hh) pathway activity plays a critical role in the growth of cancers derived from the gastrointestinal tract, including esophageal, pancreatic, biliary, and gastric cancers, and which in aggregate account for more than 10% of human cancer deaths in the USA. This ...
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Functionalized alpha 7 Nicotinic Receptor Ligands for Imaging

JHU researchers have synthesized a variety of new radiopharmaceuticals for imaging the a7 subtype of nicotinic cholingergic receptors. They are using nuclear medicine techniques, including positron emission tomography (PET) and single photon emission computed tomography (SPECT) to image these recept...
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Using Wireless Electromyographic Data to Infer Hand Applied Forces

In an effort to quantify hand-applied forces without instrumentation of the object, we have developed a force measuring system that can wirelessly transmit force-related information to a data acquisition(DAQ) unit. This system includes using electromyography (EMG) measurements of forearm and other s...
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Direct Control of the Spatial Distribution and Arrays of Micro- or Nano-meter Scale Object Across Large Areas by Fluid Evaporation on Patterned Surfaces

Our technology combines two simple processing procedures: (1) patterning surfaces with regions of differing wettability, and (2) evaporating liquid droplets containing functional nano-objects or molecules on the patterned surfaces. The combination of these procedures results in a highly parallelized...
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BRAF Mutation Occurs in a Majority of Papillary Thyroid Carcinoma

The BRAF gene was recently found to be activated by mutation in human cancers, predominantly in malignant melanoma. We tested a large number of primary tumors by PCR amplifications of BRAF exon 15 followed by restriction enzyme analysis and found a 68% frequency of a missense T to A transversion at ...
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Treatment of Metastatic Colon Cancer with B-subunit of Shiga Toxin

Colorectal cancer is the second leading cause of death from cancer in the USA (Yokota, 2000). The high mortality associated with colorectal cancer is related to its ability to spread beyond the large intestine and invade distant organs. Therefore, increasing efforts are being directed to develop way...
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Apolipoprotein C-1 Induced Apoptosis

Apolipoprotein C-I, a small basic protein in human blood, induces apoptosis in cultured human arterial smooth muscle cells through the neutral sphingomyelin (N-SMase) pathway, and the subsequent second messenger steps. An inhibitor of N-SMase abrogates this effect. Description (Set) Proposed U...
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Multiple Acquisitions of Magnetic Resonance Signal with Interleaved Inversion Pulses to Maintain the Nulling of One or More Species of Particular Longitudinal Relaxation Time(s) (T1)

In magnetic resonance imaging (MRI), one of the most common problems is to image a certain tissue without contamination from another one. For example, one is not interested in the signal coming from the fat surrounding the head while scanning the brain, or the blood when scanning the heart, etc. In ...
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Engineering RNAi Adenovirus Silencing Expression (ERASE) of DNA Repair Proteins

pERASE and Ad-ERASE (Engineered RNAi Adenovirus Silencing Expression) are respectively a plasmid and replication competent and/or deficient adenoviral vector-based systems encoding small inhibitory ribonucleic acid (siRNA). This system is designed to inhibit specific protein expression of DNA repair...
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Combined Chemical/Biological Agent Detection by Mass Spectrometry

This is a mass spectrometer for combined chemical/biological agent detection and identification that comprises (a) a combined electron impact and MALDI ionization source for volatile and non-volatile sample analyses, respectively, (b) a mode in which both EI and MALDI sources are operating simultane...
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Design and Synthesis of Renal Dipeptidase Inhibitors

Colon cancer is a deadly disease with a 6% average lifetime risk in the United States. When detected in the earliest stages, patients have a 90% survival rate. In the early stages of colon cancer, however, patients are generally asymptomatic. Therefore, colon cancer is often diagnosed in the late st...
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Non-linear Time-of-flight Mass Spectrometer

This is a unique approach to the design of time-of-flight mass spectrometers that utilizes non-linear electrostatic fields throughout the instrument. Specifically, the instrument does not include a field-free drift region. The aim of this technology is the development of miniaturized analytical inst...
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A Nucleotide Factor Kappa-B Promoter Polymorphism is associated with Increased Risk for Ulcerative Colitis

We have been investigating genes that are associated with inflammatory bowel disease. Recently, we have identified a novel IBD risk polymorphism in a regulatory region of a gene that is associated with multiple different types of inflammatory diseases. This polymorphism increases risk for ulcerative...
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Combined Chemical/Biological Agent Mass Spectrometer Detector

detection and identification of both chemical and biological agents. Specifically, it provides both conventional mass spectra of volatile chemicals and tandem, or MS/MS, measurements to characterize chemical structures for obtaining identification. As a I biological detector it provides measurements...
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Molecular Characteristics of Non-small Cell Lung Cancer

expression (SAGE) in a total of nine normal lung epithelial cells and non-small cell lung cancers (NSCLC). Separation of normal and tumor samples, as well as histopathological subtypes, was evident using the 3,921 most abundant transcript tags. This distinction remained when just 115 highly differen...
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Potential Biomarker for Use in Targeted Drug Delivery Applications as well as Other Therapeutic Strategies

Abstract (Set) According to the World Health Organization (WHO), malignant tumors accounted for around 12% of deaths worldwide, and more than 10 million new cases of cancer emerge each year. JHU scientists have identified that protein expressed by colon carcinoma cells possesses specific selectin b...
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Magnetic Resonance Imaging and Spectroscopic Imaging of pH

In situ MR imaging and spectroscopy method to detect the effect of pH and/or protein content changes on the intensity of the water signal in MRI. This is done by selectively irradiating and saturating the broad amide proton resonance of the endogenous mobile proteins centered around 8.3 ppm in the p...
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Use of Polymer-based Gene Delivery Systems for Molecular and Cellular Labeling, and pH Monitoring with MRI and MR Spectroscopy (MRS)

There is great interest in magnetic resonance imaging (MRI) contrast agents that are capable of visualizing small concentrations of molecular or cellular labels in vivo. The present invention relates to some existing polyamides (dendrimers, poly-lysine, and polyglutamate) that are extremely suitable...
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Ambulatory Raynaud's Monitor

Monitoring a person?s response to cold by recording their skin temperature is a key physiological response parameter for identifying Raynaud?s. This device would provide the means for physicians to definitively diagnose the disease without having to rely solely on a patient?s subjective feedback. Ac...
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Simultaneous DNA Sequencing Technique (SimulSeq) and Combined PCR/sequencing (AmpliSeq)

We report two novel methods for DNA sequencing. AmpliSeq permits combined PCR and cycle sequencing in a single reaction. This can be bi-directional (e.g. for mutation confirmation) or unidirectional. Full-length sequencing can be achieved. A second method, SimulSeq, permits simultaneous sequencing o...
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Beta-Secretase (BACE1) Polyclonal Antisera

To facilitate our understanding of the physiological role of BACE1 and its involvement in the generation of the Alzheimer?s disease (AD) Ab peptides, we generated a rabbit polyclonal antibody against BACE1. Our BACE1 antisera will be useful in the BACE1 levels and expression patterns in brains of no...
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A Multiple-projection DXA Scanner for Improved Accuracy and Precision in the Measurement of Bone Cross-sectional Geometry and Body Composition

Osteoporosis and a number of other diseases and conditions alter the mechanical strength of the skeleton such that bones fracture easily. A number of treatments exist that can mitigate the loss of bone strength, hence accurate diagnosis and assessment of the effects of treatment are critical. Bone-w...
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BDNF Polymorphism and Association with Bipolar Disorder

Methods for diagnosing and treating neuropsychiatric disorders, especially bipolar disorder, and to methods for identifying compounds for use in the diagnosis and treatment of neuropsychiatric disorders are disclosed. Also disclosed are novel compounds and pharmaceutical compositions for use in the ...
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A Simulation Method for Designing Customized Interventional Medical Device During Pretreatment Planning

This invention, provides a method to design and evaluate the potential performance and/or clinical application of medical devices for interventional cardiovascular applications. The design and evaluation process is based on quantitative analysis of volume rendered images, followed by a derivation of...
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Simulator for cardiovascular Image Guided Therapy

An embedded and integrated system is disclosed for augmented reality based simulation of image-guided cardiovascular interventional therapeutic procedures for training, education and certification and patient specific pre-treatment planning. The system provides users with a training and pre-treatmen...
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MR Imaging of Vascular Gene Therapy

Our current knowledge about the bio-distribution and/or in vivo pharmacokinetics of gene therapy is incomplete and relies mainly on staining of biopsied or post-mortem tissues. Modern imaging techniques provide a unique opportunity to monitor and control gene therapy. Johns Hopkins University resear...
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Identification of DNA Sequences Differentially Methylated in Pancreatic Cancer

Researchers at Johns Hopkins University have used the techniques of methylated CpG island amplification (MCA) coupled with representational difference analysis (RDA) to isolate 42 clones, which were shown to be differentially methylated in a panel of 8 pancreatic cancer cell lines compared to normal...
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The Cardiac Ausculatory Recording Database (CARD): an Internet-based software Program and Digital Heart Sound Recording Database for Teaching and Study of Cardiac Auscultation

The Cardiac Auscultatory Recording Database (CARD) was also developed. Developed using the Microsoft Access relational database program, CARD is available online through the World Wide Web. CARD contains digitized heart sound recordings collected from patients at the Pediatric Cardiology Echocardiog...
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Digital Optical Imaging for Tracking Vascular Gene Expression and Early Diagnosis of Atherosclerosis

Atherosclerotic cardiovascular disease remains the leading cause of mortality in the United States. Gene therapy is a rapidly expanding field with great potential for the treatment of atherosclerotic cardiovascular diseases. Several genes have been shown to be useful for preventing acute thrombosis,...
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A Novel Rapid Quantitative Assay for the Simultaneous Detection of Microbial Infection and Speciation

Current methods for determining whether a patient has a bacterial infection are time-consuming and may lead to delays in diagnosis and/or treatment. Real-time PCR is an emerging molecular technique, which rapidly amplifies small amounts of selected DNA regions, offering the potential for expedited i...
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Recombinant Ehrlichia Phagocytophila AnkA Protein (EPANK1 Protein) from Clone pHGE-D

Recombinant E. phagocytophilia MSP is a major immunodominant protein antigen derived from the human E. phagocytophilia (HE agent) strain BDS. The clone was initially selected based upon reactivity with monoclonal antibody to E. phagocytophilia group antigens and the gene sequenced and subcloned into...
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Rapid PCR-based blood test for Detection of Bacteria

An innovative technique that establishes a unique and highly specific assay, which eliminates ubiquitous background DNA in all PCR reagents (Taq polymerase, primers, dNTP's, PCR buffer, MgC12) by employing the use of a carefully selected restrivtion enzyme digest. This protocol thus allows for conta...
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Near-field Radio Frequency Planar Strip Array Antenna for NMR Imaging

A device for detecting electromagnetic signals, more specifically, the magnetic resonance signals from excited nuclei as well as related apparatuses, systems and methods is provided. This detection device includes a planar strip array antenna including a plurality, and in more particular embodiments...
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Bisubstrate Analog Inhibitors for Protein Kinases

A new approach for the design of protein kinase inhibitors is disclosed. Using this approach a potent insulin receptor protein tyrosine kinase inhibitor has been produced. In principle, this method is general and could be applied to any protein kinase. Previous bisubstrate analog compounds have been...
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Referenceless Interleaved Echo Planar Imaging

The patent below describes a method for obtaining magnetic resonance images using ?echo planar imaging? and related techniques, without the use of ?reference scans?. The patent describes a method for calibrating a scanner for time delays and phase shifts and using this a priori knowledge to avoid th...
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Methylated CpG Island Amplification (MCA)

Aberrant methylation of promoter region CpG islands is associated with transcriptional inactivation of tumor-suppressor genes in neoplasia. JHU scientists have developed a novel method of understanding patterns of CpG island methylation called Methylated CpG island Amplification (MCA). MCA is a PCR...
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FAMOUS: a Quantitative Assessment of Muscle Tone in the Wrist

This medical device is an automated robotic tone assessment device that non-invasively and properly quantifies tone of the flexor/extensor muscle groups that control the movement of an appendage such as muscles that control the wrist or ankle. Tone is a clinical term that describes muscle resistance...
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EEG & Evoked Potential Digital Data & Programs

Experimental recordings of evoked potential signals have been digitized and stored on computer disks & CDs. Programs have been written to analyze and display the data and its subsequent interpretation. Description (Set) Proposed Use (Set) The recordings and programs could be used for further r...
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A Method for Detection of Cancer and/or Risk of Cancer Based on Loss of Imprinting

At present, genetic testing is able to detect only a small fraction of the population at risk of cancer. For example, FAP mutations are present in less than 1% of the population, and hereditary nonpolyposis colorectal cancer mutations are present at only about 0.1% prevalence. A method has been deve...
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Identification of the Human Fast-1 Gene

In Xenopus embryos, a subset of transcriptional responses to the TGF-B family of ligands is mediated by the Xenopus forkhead activin signal transducer-1 (xFAST-1). We have now identified a human FAST-1 homolog (hFAST-1). Although significantly different in sequence from its Xenopus counterpart, hFA...
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Passive Intraocular Pressure Sensors & Patient-Worn Monitorying Recorder

A device for passively measuring intraocular pressure of a patient including an in vivo sensor and an instrument external to the patient for remotely energizing the sensor, thereby permitting the instrument to determine the intraocular pressure. The device directly and continuously measures the intr...
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GDF - 15

The present invention provides a growth and differentiation factor, GDF-15, and a polynucleotide sequence encoding GDF-15. In one embodiment, the invention provides a method for detection of a cell proliferative or immunologic disorder which is associated with GDF-15 expression or function. In anoth...
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High Frequency Retrotransposition in Cultured Mammalian Cells

Two human L1 elements can actively retrotranspose in cultured mammalian cells. When stably expressed from an episome in HeLa cells, both elements retrotransposed into a variety of chromosomal locations at a high frequency. The retrotransposed products resembled endogenous L1 insertions, since they w...
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Method for Measuring Myocardial Motion

A method of magnetic resonance imaging of myocardial motion includes positioning a portion of a patient such as a patient's heart within a main magnetic field and employing a plurality of RF signals to establish a plurality of parallel plane tags through the portion. In imaging heart motion, a first...
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A Test for the Diagnosis of Polycythemia Vera

Polycythemia vera (PV) is a stem cell disorder characterized as a panhyperplastic, malignant, and neoplastic marrow disorder. The most prominent feature of this disease is an elevated absolute red blood cell mass because of uncontrolled red blood cell production. This is accompanied by increased whi...
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Novel Genes of Kaposi's Sarcoma Associated Herpesvirus (Human Herpesvirus Eight)

occurring in AIDS patients and closely associated with Karposi�s sarcoma (KS), body cavity based lymphomas (BCBL), multiple myeloma, and multicentric Castleman�s disease (MCD). JHU scientists have discovered several novel viral open reading frames (ORF�s) for KSHV. Description (Set) Proposed Use...
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Use of Recombinant Bacteriophages to Monitor Ion Channel Receptor Expression im Mammalian Cells

The present invention concerns a novel method for detecting cell surface proteins utilizing a modified virus. This method combines peptide display technology and traditional immuno-amplification strategy to provide an economical detection system. In contrast to traditional approaches, both specifici...
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Discovery of Human Mad-related Genes

Resistance to the growth inhibitory effects of TGF-beta is common in human cancers. However the mechanism(s) by which tumour cells become restistant to TGF-beta are generally unknown. We have identified five novel human genes related to a Drosophila gene called Mad which is thought to transduce sign...
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Novel Peptide Modulators for NMDA Glutamate Receptor

The invention concerns the identification of a novel ligand binding site on a mammalian NMDA receptor. The invention further provides for NMDA receptor ligands, pharmaceutical compositions and novel drug screens...
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Method for Assessing Cardiac Contractility and Cardiovascular Interaction that may be Applied to Non-Invasive Clinical Practice

It has been found that E.sub.N (t.sub.N) curves are surprisingly similar among hearts, particularly during early contraction. Based on this observation, the present invention provides an arrangement (method and apparatus) for determining a good approximation of ESPVR using pressure, volume and timin...
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An Assay for Determining Subunit Interaction in Ion Channels

The present invention relates to compounds and related methods for both inhibiting and potentiating potassium ion channel activity. In particular, a molecular code of the alpha-subunit in a Shaker-like potassium ion channel was identified that provides for the beta-subunit-mediated regulation of pot...
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Urease-Responsive Delivery Systems for Diagnostic and Therapeutic Applications

The present invention provides novel compositions and methods for diagnosing the presence of Helicobacter pylori infections of the stomach or duodenum in humans or other mammals. The invention provides a urease responsive system for the delivery of diagnostic marker or therapeutic compound. In one e...
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DPC4, a Candidate Tumor-Suppressor Gene

The DPC4 gene is also known as SMAD4 or as MADH4. It is a mediator of signal transduction for TGFbeta, activin, bone morphogenic proteins, and similar extracellular growth and differentiation ligands. DPC4 conducts a signal that suppresses tumorigenesis. Mutations in the DPC4 gene are found in most ...
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Platelet Polymorphism PI-A2

A method for diagnosing a subject having or at risk of having a thrombotic disease syndrome is provided by analysis of a platelet polymorphism. Specifically, the association between polymorphism of the P1A2 allele of the GPIIIa gene and unstable thrombotic syndromes provides the basis for methods an...
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Cross-Linked Extra Cellular Matrix Polymers for Enzyme Detection

Proteoglycans are found throughout the body and serve numerous structural and functional roles. Chondroitin sulfate (CS) was modified to varying degrees with (meth)acrylate groups (CSMA) and polymerized to form a crosslinked hydrogel. Depending on the degree of modification, swelling, mechanical, an...
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Cryo Filter Exchanger

This device maintains parallelism between diffracting crystals while allowing the crystals to continuously travel through high to low take-off angles. A central shaft that rotates by one half of the value of the take-off angle drives the device. The central shaft allows for variable collimation to t...
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Volatile Biomarkers for Analysis of Hepatic Disorders

The present invention features test systems and methods for detecting a hepatic disorder in a mammal and especially a primate. Preferred use of the invention involves staging the hepatic disorder in a human patient. Patent (Set) 6,248,078...
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Apparatus for the Separation of Cystic Parasite Forms from Water

A continuous flow centrifuge adapted with an insert of glass beads, sand or the like is used to concentrate cysts of microorganisms such as giardia or cryptosporidium from large volumes of water. The apparatus and methods are useful for the detection of contaminating microoorganisms in groundwater a...
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Apparatus and Method for Exercising and Measuring Strength of a Patient's Limb

An apparatus for measuring the strength of and for performing physical therapy exercises to strengthen a patient's limb. The device includes a pair of pivot clamps each having a end for connecting the pivot clamps to a solid object such as a physical therapy table or hospital bed. A second end of ea...
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Monoclonal Antibodies (AE-1) Specific for Cytoskeletal Proteins useful for Tumor Diagnosis

Differential diagnosis of tumors can sometimes be difficult. For example, the histological appareance of thymoma (epithelial-derived) and histicytic lymphoma (mescenchyme-derived) can be similar. Since the mode of treatment and prognosis for these two types of tumors are significantly different, an ...
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Mixing Cell for Microcalorimeters

The present invention relates to a device and method for measuring the heat of reaction resulting from the mixture of a plurality of liquid reagents. The heat of reaction is one of the most important thermochemical characteristics of any reaction and is widely used in fundamental research and appli...
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Early Detection of Lung Cancer Treatment

The present invention relates to a method for early detection of lung cancer. The method comprises obtaining samples from the body, especially respiratory tract material including sputum or bronchial fluid or any other pulmonary tissue or thoracic cells or regional lymph nodes, and assaying the samp...
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Nuclear Magnetic Resonance Spectroscopy Data Acquisition Schemes for Phase-Sensitive Detection of Pure Absorption Mode Signals Devoid of Dispersive Components Arising from Phase Errors

The methodology described in this disclosure enables one, for the first time, to remove dispersive components arising from phase errors (inevitably) introduced during acquisition of multi-dimensional spectra. This enables one to measure NMR paramete rs such as chemical shifts more accurately, and au...
...

Nuclear Magnetic Resonance Spectroscopy Methods for single Transient Data Acquisition without Use of Readout Pulsed Field Gradients

Nuclear Magnetic Resonance Spectroscopy Methods for single Transient Data Acquisition without Use of Readout Pulsed Field Gradients Categories: Research Tool, Diagnostic...
...

Bridging Centrality: a Concept and Formula to Identify Bridging Nodes in Scale-free Networks

The invention relates to a novel essential component identification model, bridging centraility, based on information flow and topological locality in scale-free networks. Bridging centrality provides an entirely new way of scrutinizing network struc tures and measuring components' importance. The ...
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Aromatic Proton Relaxation Optimized Protein NMR Spectroscopy

In recent years, rapid NMR data collection has emerged as a pivotal area of research in structural biology. This invention describes the implementation of a longitudinal relaxation of aromatic protons (which increases the sensitivity of NMR experime nts). This invention also allows one to collect in...
...

G-Matrix Fourier transform NMR experiments for resonance assignment and structure determination of organic molecules

The suite of G-Matrix Fourier Transform experiements described can be used for complete resonance assignments of proteins and to obtain structural constraints for determining high resolution 3D structures of biomolecules. Categories: Research Tool, Diagnostic...
...

A Rabbit Polyclonal Antibody Against Parkin

Since mutations of the parkin gene are the most frequent cause of familial Parkinson’s Disease (PD) and because heterozygous parkin mutations can also be found in sporadic PD, there is growing interest in studying this gene and its product. A UB res earcher has generated a high titer, rabbit polyclo...
...

New GFT NMR Experiments for Complete Resonance Assignment of Proteins

This invention describes a set of new GFT NMR experiments for the assignment of backbone and side-chain resonances of proteins. The invention applies a Gaussian Matrix to the data acquisition and processing methods, creating the ability to adjust th e investments of NMR sampling time to the actual d...
...

GFT NMR Spectra for Rapid Assignment and Secondary Structure Determination of Proteins

This invention describes the application of two previous inventions (5770 GFT NMR Spectroscopy and 5655 RD NMR Spectroscopy) to form a suite of four experiments. These improve measurement accuracy, as well as reduce NMR sampling time. They also all ow highly efficient assignment of backbone and 13Cß...
...

Phase Sensitively Detected Reduced-Dimensionality NMR Spectra for Rapid Assignment and Secondary Structure Determination of Proteins

UB researchers have developed a novel NMR data acquisition and processing scheme named GFT nuclear magnetic resonance (NMR) Spectroscopy, where ‘GFT’ stands for a combined G-matrix and Fourier transformation. GFT NMR allows one to acquire higher dime nsional NMR spectral information orders of magnit...
...

GFT NMR Spectroscopy

The minimal measurement time of an N dimensional FT NMR experiment is constrained by the need to sample N-1 indirect dimensions and may therefore exceed by far the time required to achieve workable signal-to-ratios. This invention describes a no vel approach named "GFT NMR spectroscopy" for data...
...

CT Mammography Detector

Computed tomography (CT) uses x-ray imaging to obtain cross-sectional images of the body that can then be integrated into a three dimensional image. Unlike conventional x-rays, CT scans can distinguish clearly overlying structures and tissues includ ing bone, soft tissue, and blood vessels. Because ...
...

Single frame laser scan ratio imaging using two wavelength excitation

For two distinct chromophore populations to be imaged and their ratio computed, currently two different laser excitations are raster scanned and alternately switched after each frame of data acquisition. Since there is a time lag (typically 100s of m illiseconds) between the imaging of the two, dyna...
...

Thermal Imaging at micrometer resolution

Thermal imaging (visual reproduction of thermal gradients within a body) is a mature science. Several instruments exist that are variants of infra-red (IR) radiation detection cameras. But with IR wavelengths starting off (~ 1 micron) where sizes of most biological microsystems (cells) end, current ...
...

Region of Interest Micro-Angiography

In cerebro-vascular interventions, smaller and smaller catheters, guide wires, and interventional devices such as stents and coils are being used which are difficult to visualize with conventional imaging systems. The present invention provides a cha nge in the design paradigm for radiographic detec...
...

CryoTissue Conditioner (Conditioner Solution for Frozen Tissue Sections for Histological Examination)

Microscopic examination of tissue morphology and intracellular protein content is one of the major methods of assessing disease states in tissue samples. Current techniques often result in damage to the tissue section either during the cryostat sect ioning or as the sections are subjected to shear s...
...

A Method to Distinguish Organisms Causing Exacerbations of Chronic Bronchitis

The invention provides a simple, quick and inexpensive test for discriminating between bacterial and non-bacterial exacerbations of chronic lung disease. The test is based on the strong correlation between bacterial AECB (acute exacerbations of chro nic bronchitis) and sputum elastase. The test comp...
...

Novel and Enhanced approaches to determine local mRNA accessibility for Development of Post-Transcriptional Gene Silencing Agents

Bioinformatics approach for determining mRNA sites which are good candidates for RNAi or ribozyme sites. Categories: Genomics, Diagnostic...
...

Identification of Lead Candidate Ribozymes for Human Rod Opsin Therapeutics

Highly effective hammerhead ribozyme sequenes for reducing rod opsin mRNA levels. Categories: Genomics, Diagnostic...
...

High Throughput Cellular Screening for Development of Post-Transcriptonal Gene Silencing Agents against Arbitrary mRNA Targets

High Throughput Cellular Screening for Development of Post-Transcriptonal Gene Silencing Agents against Arbitrary mRNA Targets SEAP-based HTP screening method for ribozyme sites on mRNA targets. Categories: Genomics, Diagnostic...
...

Nuclear Magnetic Resonance Spectroscopy Data Acquisition Schemes for Phase-Sensitive Detection of Pure Absorption Mode Signals Devoid of Dispersive Components Arising from Phase Errors

The methodology described in this disclosure enables one, for the first time, to remove dispersive components arising from phase errors (inevitably) introduced during acquisition of multi-dimensional spectra. This enables one to measure NMR paramete rs such as chemical shifts more accurately, and au...
...

Nuclear Magnetic Resonance Spectroscopy Methods for single Transient Data Acquisition without Use of Readout Pulsed Field Gradients

Nuclear Magnetic Resonance Spectroscopy Methods for single Transient Data Acquisition without Use of Readout Pulsed Field Gradients Categories: Research Tool, Diagnostic...
...

Novel and Enhanced approaches to determine local mRNA accessibility for Development of Post-Transcriptional Gene Silencing Agents

Bioinformatics approach for determining mRNA sites which are good candidates for RNAi or ribozyme sites. Categories: Genomics, Diagnostic...
...

High Throughput Cellular Screening for Development of Post-Transcriptonal Gene Silencing Agents against Arbitrary mRNA Targets

SEAP-based HTP screening method for ribozyme sites on mRNA targets. Categories: Genomics, Diagnostic...
...

Use of RNA inhibitors based on IL-14 and antibodies to IL-14 for the treatment of autoimmune diseases and lymphomas

Autoimmune disorders and B cell derived lymphomas cause life-long suffering or premature death. Often the treatment for these diseases is symptomatic at best with severe side effects. An effective new treatment would be life saving for those patie nts. The cytokine interleukin-14 (IL-14) also known ...
...

Non-Invasive Screening Device for Upper Aerodigestive Tract Neoplasia

Diagnostic medical equipments are probably one of the most important paraphernalias of the medical field. This is because of the fact that in all the medical procedures, diagnosis is the first step towards combating diseases. It assumes critical impo rtance when the diseases are of the nature that r...
...

Aromatic Proton Relaxation Optimized Protein NMR Spectroscopy

In recent years, rapid NMR data collection has emerged as a pivotal area of research in structural biology. This invention describes the implementation of a longitudinal relaxation of aromatic protons (which increases the sensitivity of NMR experime nts). This invention also allows one to collect in...
...

CT Mammography Detector

Computed tomography (CT) uses x-ray imaging to obtain cross-sectional images of the body that can then be integrated into a three dimensional image. Unlike conventional x-rays, CT scans can distinguish clearly overlying structures and tissues includ ing bone, soft tissue, and blood vessels. Because ...
...

CryoTissue Conditioner (Conditioner Solution for Frozen Tissue Sections for Histological Examination)

Microscopic examination of tissue morphology and intracellular protein content is one of the major methods of assessing disease states in tissue samples. Current techniques often result in damage to the tissue section either during the cryostat sect ioning or as the sections are subjected to shear s...
...

G-Matrix Fourier transform NMR experiments for resonance assignment and structure determination of organic molecules

The suite of G-Matrix Fourier Transform experiements described can be used for complete resonance assignments of proteins and to obtain structural constraints for determining high resolution 3D structures of biomolecules. Categories: Research Tool, Diagnostic...
...

Use of anti-T antibody to block metastasis of T antigen bearing tumors

Many cancer cells contain a carbohydrate structure on their surface known as the Thomsen-Friedenreich Antigen (TF-Ag). This antigen, which is not detectable on normal cells, plays a pivotal role in metastasis. An antibody which binds strongly to TF -Ag would be extremely useful in imaging cancer in ...
...

Single frame laser scan ratio imaging using two wavelength excitation

For two distinct chromophore populations to be imaged and their ratio computed, currently two different laser excitations are raster scanned and alternately switched after each frame of data acquisition. Since there is a time lag (typically 100s of m illiseconds) between the imaging of the two, dyna...
...

Single frame laser scan ratio imaging using two wavelength excitation

For two distinct chromophore populations to be imaged and their ratio computed, currently two different laser excitations are raster scanned and alternately switched after each frame of data acquisition. Since there is a time lag (typically 100s of m illiseconds) between the imaging of the two, dyna...
...

Thermal Imaging at micrometer resolution

Thermal imaging (visual reproduction of thermal gradients within a body) is a mature science. Several instruments exist that are variants of infra-red (IR) radiation detection cameras. But with IR wavelengths starting off (~ 1 micron) where sizes of most biological microsystems (cells) end, current ...
...

Holographic formation of periodic polymeric photonic bandgap structures for visible to IR wavelengths

Chemical and biological sensor developers have held an interest in nanoscale construction of periodic structures. Numerous optical fiber products that utilize nanoscale periodic etching are available for stress/strain sensing. Chemical sensing usi ng affinity coatings on optical fibers is still an e...
...

Monoclonal antibody 3F5-5E5 and P107 diagnostic markers and putative vaccine antigen for Moraxella catarrhalis and supplemental Mab 4G5

This invention includes monoclonal antibodies, proteins, nucleic acid seqeunces and lipoologosaccharides (LOS) which are specific to Moraxella catarrhalis. These materials are useful for designing a much-needed diagnostic test. The ability to diagn osis this form of otitis media would be very valuab...
...

New GFT NMR Experiments for Complete Resonance Assignment of Proteins

This invention describes a set of new GFT NMR experiments for the assignment of backbone and side-chain resonances of proteins. The invention applies a Gaussian Matrix to the data acquisition and processing methods, creating the ability to adjust th e investments of NMR sampling time to the actual d...
...

Research Reagents for Human Serum Paraoxonase Status

PON1 is a glycoprotein that plays a role in both metabolism of organophosphate pesticides and in the cardioprotective effect of high-density lipoprotein (HDL). Two mutant forms of the protein are known. One of these, PON1-192 has been extensively s tudied and has been shown to affect the activity of...
...

GFT NMR Spectra for Rapid Assignment and Secondary Structure Determination of Proteins

This invention describes the application of two previous inventions (5770 GFT NMR Spectroscopy and 5655 RD NMR Spectroscopy) to form a suite of four experiments. These improve measurement accuracy, as well as reduce NMR sampling time. They also all ow highly efficient assignment of backbone and 13Cß...
...

Phase Sensitively Detected Reduced-Dimensionality NMR Spectra for Rapid Assignment and Secondary Structure Determination of Proteins

UB researchers have developed a novel NMR data acquisition and processing scheme named GFT nuclear magnetic resonance (NMR) Spectroscopy, where ‘GFT’ stands for a combined G-matrix and Fourier transformation. GFT NMR allows one to acquire higher dime nsional NMR spectral information orders of magnit...
...

GFT NMR Spectroscopy

The minimal measurement time of an N dimensional FT NMR experiment is constrained by the need to sample N-1 indirect dimensions and may therefore exceed by far the time required to achieve workable signal-to-ratios. This invention describes a no vel approach named "GFT NMR spectroscopy" for data...
...

A Method to Distinguish Organisms Causing Exacerbations of Chronic Bronchitis

The invention provides a simple, quick and inexpensive test for discriminating between bacterial and non-bacterial exacerbations of chronic lung disease. The test is based on the strong correlation between bacterial AECB (acute exacerbations of chro nic bronchitis) and sputum elastase. The test comp...
...

Region of Interest Micro-Angiography

In cerebro-vascular interventions, smaller and smaller catheters, guide wires, and interventional devices such as stents and coils are being used which are difficult to visualize with conventional imaging systems. The present invention provides a cha nge in the design paradigm for radiographic detec...
...

HIV antisense ORF(s) and putative protein(s)

The current invention is a gene comprising an open reading frame on the plus strand of the pro-viral DNA, and located in the region of HIV-1 long terminal repeat. The gene encodes a protein that is related to, and has a structural motif resembling t hat of chemokine proteins. Depending upon the ribo...
...

Outer Membrane Protein B1 of Moraxella Catarrhalis

The present invention includes a method for the isolation and purification of outer membrane protein B1 as well as B1 peptides from Moraxella catarrhalis, and the resulting compositions. The method involves growing the bacteria in iron-depleted medi um to enhance the expression of the B1 protein, ha...
...

Detection of Porphyromonas Gingivalis

This invention is an accurate and sensitive system to detect the presence of Porphyromonas gingivalis, a bacterial strain associated with the development of periodontal disease. The bacteria are detected by identifying the presence of DNA specific t o the strain. This is accomplished via the polymer...
...

Non-Invasive Screening Device for Upper Aerodigestive Tract Neoplasia

Diagnostic medical equipments are probably one of the most important paraphernalias of the medical field. This is because of the fact that in all the medical procedures, diagnosis is the first step towards combating diseases. It assumes critical impo rtance when the diseases are of the nature that r...
...

Holographic formation of periodic polymeric photonic bandgap structures for visible to IR wavelengths

Chemical and biological sensor developers have held an interest in nanoscale construction of periodic structures. Numerous optical fiber products that utilize nanoscale periodic etching are available for stress/strain sensing. Chemical sensing usi ng affinity coatings on optical fibers is still an e...
...

Use of RNA inhibitors based on IL-14 and antibodies to IL-14 for the treatment of autoimmune diseases and lymphomas

Autoimmune disorders and B cell derived lymphomas cause life-long suffering or premature death. Often the treatment for these diseases is symptomatic at best with severe side effects. An effective new treatment would be life saving for those patie nts. The cytokine interleukin-14 (IL-14) also known ...
...

Use of anti-T antibody to block metastasis of T antigen bearing tumors

Many cancer cells contain a carbohydrate structure on their surface known as the Thomsen-Friedenreich Antigen (TF-Ag). This antigen, which is not detectable on normal cells, plays a pivotal role in metastasis. An antibody which binds strongly to TF -Ag would be extremely useful in imaging cancer in ...
...

Monoclonal antibody 3F5-5E5 and P107 diagnostic markers and putative vaccine antigen for Moraxella catarrhalis and supplemental Mab 4G5

This invention includes monoclonal antibodies, proteins, nucleic acid seqeunces and lipoologosaccharides (LOS) which are specific to Moraxella catarrhalis. These materials are useful for designing a much-needed diagnostic test. The ability to diagn osis this form of otitis media would be very valuab...
...

Outer Membrane Protein B1 of Moraxella Catarrhalis

The present invention includes a method for the isolation and purification of outer membrane protein B1 as well as B1 peptides from Moraxella catarrhalis, and the resulting compositions. The method involves growing the bacteria in iron-depleted medi um to enhance the expression of the B1 protein, ha...
...

Respiratory Detection System (RDS)

Introduction Respiration rates are an important indicator of cellular and metabolic states in cells ranging from mammalian to bacterial, from individuals to large populations. Unfortunately, conventional techniques have significant drawbacks: for instance, electrochemical techniques have low sensiti...
...

Surface-Bound Double-Stranded DNA Protein Arrays

Summary Developed at Harvard Medical School, this invention provides nucleic acid arrays specifically designed for the assay of DNA-dependent protein:protein binding and/or physical interactions between proteins and nucleic acid molecules. Intermolecular analyses enabled by this technology are advan...
...

MicroArray Spot LINEar Regression (MASLINER) software

Summary Signal intensities between samples on a microarrary typically vary by as much as three to four orders of magnitude, frustrating efforts at comparative analysis of samples assayed in parallel. At any given laser power or photomultiplier tube gain (PMTG), some signals will be below detection t...
...

Multiplexing long DNA syntheses

Summary The invention enables multiplex gene synthesis in a single reaction, using very low concentrations of oligonucleotides. The method, which employs a unique temporary tagging strategy, offers significant advantages in terms of speed and economy over existing methods for building genes, and eve...
...

A Microconcentrator and Microfilter for Suspensions such as Blood

Summary Background: In healthcare, chemical analysis, environmental testing and other fields it is useful to separate particulate substances that are suspended in a fluid. For example separating cells from body fluids. So far, these techniques have typically required substantial capital equipment...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Summary Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow thr...
...

Targeting FtsZ Activity Using Novel Antibacterials: Screening Assays, Small Molecule Hits, and Synthetic Pathways to Potent Natural Products

Summary The emergence of bacterial strains resistant to current drugs has prompted a renewed effort to discover new methods for fighting infectious disease. A promising new target is FtsZ, a key mediator of bacterial cell division that is highly conserved among bacteria. Because FtsZ, much like its ...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow through a m...
...

Use of Cytokine IP-10 as an Anti-Tumor Agent

Summary The invention provides compositions and methods for the diagnosis and treatment of both malignant and benign cell-proliferative diseases. When complexed to its receptor, IP-10 interacts with known cytokines, including FGF and TGFb; this interaction down-regulates cell division and/or results...
...

Probe of Regional Lung Function

Summary This invention provides a direct means for measuring the dynamic properties of small regions of the lung of a living animal in real time. The parameters of lung function which may be analyzed include: (i) the surface area to volume ration, (ii) the pulmonary capillary blood volume density an...
...

Home-Based Monitoring Addressing High-Risk Cardiovascular Conditions and Sleep Apnea

Summary Sleep may trigger a variety of adverse conditions in patients with heart and/or respiratory ailments. While numerous sleep apnea monitors have been developed, a critical unmet need exists for effective nocturnal monitoring of cardiac events in high-risk heart disease patients and severe slee...
...

Production and uses of polymerase colony ("polony") arrays

Summary The invention provides methods for rapid fabrication and use of replicable, high-density nucleic acid arrays. Array features are produced through amplification on a support matrix of nucleic acid templates of interest. A significant advance over conventional DNA array technologies, the so-ca...
...

Surface-Bound Double-Stranded DNA Protein Arrays

Summary Developed at Harvard Medical School, this invention provides nucleic acid arrays specifically designed for the assay of DNA-dependent protein:protein binding and/or physical interactions between proteins and nucleic acid molecules. Intermolecular analyses enabled by this technology are advan...
...

A Tool for the Linkage and Presentation of Multiple Protein Functional Domains

Summary Recent molecular biology research is moving beyond genetics to look at protein - protein interactions and how cells interact or communicate. These exciting areas are in need of improved tools that allow these interactions to be identified and studied. Harvard Medical School researchers h...
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Vaccine Compositions AMPERSAND Methods for Human Papilloma Virus

Summary This invention comprises methods of expressing the Human Papilloma Virus L1 and L2 proteins, and variations thereof, in bacterial host cells; the purification of the expressed proteins; and their use in the development and application of vaccine and diagnostic technologies. Applications F...
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Valpha24JalphaQ Receptor: Lead Diagnostic and Therapeutic for Type I Diabetes

Summary Background: Autoimmune diseases are the result of a patient's immune system attacking their own cells and tissues. This can result in a wide variety of diseases, including multiple sclerosis, myasthenia gravis, and type 1 diabetes. Researchers at Harvard University have discovered a novel me...
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Use of glycated CD59 levels to assess glycemic control in diabetic patients

Summary The invention enables rapid detection of impaired glucose tolerance (IGT, or “pre-diabetes”) without oral glucose tolerance testing and permits identification of a population at risk of developing the vascular complications associated with diabetes. It also offers a robust tool for monit...
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Single-Cell Transcriptional Analysis with Microarrays

Summary The enormous tissue and cellular diversity of multicellular organisms poses unique challenges, such as learning the gene expression patterns associated with embryonic or neurological development. One tool scientists use to help address these problems is DNA microarrays, which help provide me...
...

Next Generation Diagnostics for Breast Cancer, Prostate Cancer, and Melanoma

Summary From the labs of Professor Alun Jones (inventor of CardioliteTM) and Professor Ashfaq Mahmood, comes a series of small molecule imaging probes, capable of conjugation to both SPECT-based and PET-based imaging agents. Lead candidates include sigma-1 and selective sigma-2 receptor probes in th...
...

A Versatile Method for Accelerated Chromosome Walking and DNA Fingerprinting

Summary Background: A variety of DNA amplification methods deal with sequence analysis of an uncharacterized region adjacent to a known element. These methods include inverse PCR (iPCR), panhandle PCR, cassette ligation-anchored, and Thermal Asymmetric Interlaced PCR. Despite various degrees of succ...
...

Microcapillary Column Peptide Trap

Summary Protein detection using liquid chromatography (LC) followed by tandem mass spectroscopy has become a valuable tool for large-scale proteome analysis. Many efforts have been made to enhance throughput while maintaining sensitivity, particularly in the realm of low-volume samples. One such eff...
...

Algorithm for Haplotype Construction of Single Nucleotide Polymorphism

Summary The Haplotyper software program utilizes a novel algorithm called Partition-Ligation to reconstruct individual haplotype phase information from unphased genoptype data. The Partition-Ligation method uses an ingenious Markov chain Monte Carlo approach both to construct the partial haplotypes ...
...

Susceptibility to Tuberculosis 1 (sst1): Novel Target for Diagnosis and Treatment of Mycobacterium tuberculosis

Summary Background: Tuberculosis is the seventh most important cause of global premature mortality and disability. Currently there are 8 million new cases and 3 million deaths annually from tuberculosis, and it is projected that a total of 225 million new cases and 79 million deaths will occur betwe...
...

Multiplexing long DNA syntheses

Summary The invention enables multiplex gene synthesis in a single reaction, using very low concentrations of oligonucleotides. The method, which employs a unique temporary tagging strategy, offers significant advantages in terms of speed and economy over existing methods for building genes, and eve...
...

Bead-based polymerase colony microarrays and nucleic acid sequencing ("Polony-FISSEQ Beads")

Summary Foundational work of Dr. George Church has enabled the production and use of replicable arrays of polymerase-amplified nucleic acid colonies (so-called "polonies") on semisolid support matrices (see Harvard Case No. 1438). The present invention extends the polony technology to provided minia...
...

Amplification of trace amounts of nucleic acids

Summary The importance of nucleic acid amplification from trace amounts of biological sample has increased with the expansion of genomic sequencing in various clinical and research-oriented applications. This invention characterizes techniques to reduce background signal experienced while cloning su...
...

Fully-integrated 2D microelectromagnet coil arrays in silicon for magnetic manipulation and RF imaging of cells and magnetic particles

Summary The invention comprises a novel method and an apparatus to manipulate, segregate, detect and analyze biological samples on a microfluidic CMOS chip. This technique offers sub-micrometer precision and fast processing speeds for a cheap, easily accessible lab-on-a-chip system. The technology i...
...

A Microconcentrator and Microfilter for Suspensions such as Blood

Background: In healthcare, chemical analysis, environmental testing and other fields it is useful to separate particulate substances that are suspended in a fluid. For example separating cells from body fluids. So far, these techniques have typically required substantial capital equipment that ca...
...

Mass spectrometric methods of determining levels of glycated CD59

Summary CD59 is a protein that exists in both glycated and non-glycated forms. An elevated ratio of glycated to non-glycated CD59 has been found to be an early (i.e., pre-symptomatic) prognostic marker for the onset of diabetes, as well as for its devastating vascular complications. In addition to i...
...

Proteasome-Mediated Degradation of RISC: Lead Target for Treatment of Long-Term Memory Disorders

Summary Background: Breakthrough research by Professor Sam Kunes and colleagues at Harvard University demonstrates that degradative control of the miRNA RISC pathway underlies the synaptic pattern of protein synthesis associated with the establishment of a stable long-memory. Manipulation of this pa...
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Detection of Invasive Bladder Cancer by DNA Hypermethylation

Summary Background: The National Cancer Institute has estimated that in year 2008, 68,810 new cases of bladder cancer will be diagnosed in United States, causing 14,100 deaths. The mortality of this cancer is attributed to the invasiveness of solid tumors into the muscular layers of the bladder. Cli...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Summary Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow thr...
...

Targeting FtsZ Activity Using Novel Antibacterials: Screening Assays, Small Molecule Hits, and Synthetic Pathways to Potent Natural Products

Summary The emergence of bacterial strains resistant to current drugs has prompted a renewed effort to discover new methods for fighting infectious disease. A promising new target is FtsZ, a key mediator of bacterial cell division that is highly conserved among bacteria. Because FtsZ, much like its ...
...

Enhanced Nanoparticle-Based Molecular Imaging via MRI

Summary Enhanced Nanoparticle-Based Molecular Imaging via MRI Elevator pitch: For biotech and pharmaceutical companies who need to track drug effectiveness in the drug discovery and for MRI facilities, hospitals and physicians who seek to diagnose and treat specific diseases through molecular im...
...

Novel Biomarkers for Chronic Myelogenous Leukemia (CML)

Summary A high-dimensional metabolomics screen to diagnose and monitor chronic myeloid leukemia (CML) has been developed. Over 3000 metabolites are detected to yield a ‘cytogenetic signature’ that confirms the presence of cells bearing the Philadelphia chromosome, a genetic marker for CML. Throu...
...

A Novel Screening Method for Assessment of Psychopathologic Disorders

Summary Background: The advancement in psychiatric genetics and clinical neuroscience is hindered by the heterogeneity of psychiatric disorders and by difficulties in defining and characterizing the phenotype under investigation. A promising endophenotype is anhedonia, the loss of pleasure or lack o...
...

Identification of Contactins and L1-CAMs as ligands for the Amyloid Precursor Protein

Summary Approximately 24 million people worldwide have dementia of which the majority (~60%) is due to Alzheimer’s; by 2040, it is projected that this figure will have increased to 81 million. The drugs currently on the market, such as cholinesterase inhibitors, reduce the cognitive symptoms of Al...
...

Combinatorial Allelic Anaalysis via Multiplex Automaged Genome Engineering ("MAGE")

Summary Applications Contact Maryanne Fenerjian, PhD Office of Technology Development Tel: 617-432-3845 Email: maryanne_fenerjian@harvard.edu For Further Information Please Contact the Director of Business Development Maryanne Fenerjian Email: maryanne_fenerjian@hms.harvard.edu Telephone: (617) ...
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Compounds and Methods for Tumor-Specific Targeting

Summary A novel strategy for developing cancer-homing drugs has been developed by Dr. Amin Kassis that combines Enzyme-Mediated Cancer Imaging and Therapy (EMCIT) with radionuclides (see figure below). This powerful method achieves a multimodal effect, since it can be exploited for therapeutic effic...
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Biological Computers: in Vivo Autonomous Doctors that Diagnose and Treat Disease

Summary Background: The promise of computers made from biological molecules lies in their potential to operate within the biochemical environment of a living organism and to interact with that environment through inputs and outputs with other biological molecules. For example, a biomolecular compute...
...

C16:1n7-palmitoleate: a novel lipid hormone as a diagnostic and therapeutic tool

Summary Markets / Needs Addressed: Insulin resistance is the key factor of a collection of metabolic disorders including Type II diabetes, atherosclerosis, fatty liver disease, hypertension and dyslipidemia. The syndrome has reached epidemic levels worldwide, with 115 million individuals in 2004 suf...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Summary Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow thr...
...

Biological Computers: in Vivo Autonomous Doctors that Diagnose and Treat Disease

Summary Background: The promise of computers made from biological molecules lies in their potential to operate within the biochemical environment of a living organism and to interact with that environment through inputs and outputs with other biological molecules. For example, a biomolecular compute...
...

Fully-integrated 2D microelectromagnet coil arrays in silicon for magnetic manipulation and RF imaging of cells and magnetic particles

Summary The invention comprises a novel method and an apparatus to manipulate, segregate, detect and analyze biological samples on a microfluidic CMOS chip. This technique offers sub-micrometer precision and fast processing speeds for a cheap, easily accessible lab-on-a-chip system. The technology i...
...

Home-Based Monitoring Addressing High-Risk Cardiovascular Conditions and Sleep Apnea

Summary Sleep may trigger a variety of adverse conditions in patients with heart and/or respiratory ailments. While numerous sleep apnea monitors have been developed, a critical unmet need exists for effective nocturnal monitoring of cardiac events in high-risk heart disease patients and severe slee...
...

rocapillary Column Peptide Trap

Summary Protein detection using liquid chromatography (LC) followed by tandem mass spectroscopy has become a valuable tool for large-scale proteome analysis. Many efforts have been made to enhance throughput while maintaining sensitivity, particularly in the realm of low-volume samples. One such eff...
...

MicroArray Spot LINEar Regression (MASLINER) software

Summary Signal intensities between samples on a microarrary typically vary by as much as three to four orders of magnitude, frustrating efforts at comparative analysis of samples assayed in parallel. At any given laser power or photomultiplier tube gain (PMTG), some signals will be below detection t...
...

Fully-integrated 2D microelectromagnet coil arrays in silicon for magnetic manipulation and RF imaging of cells and magnetic particles

Summary The invention comprises a novel method and an apparatus to manipulate, segregate, detect and analyze biological samples on a microfluidic CMOS chip. This technique offers sub-micrometer precision and fast processing speeds for a cheap, easily accessible lab-on-a-chip system. The technology i...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Summary Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow thr...
...

A Novel Screening Method for Assessment of Psychopathologic Disorders

Summary Background: The advancement in psychiatric genetics and clinical neuroscience is hindered by the heterogeneity of psychiatric disorders and by difficulties in defining and characterizing the phenotype under investigation. A promising endophenotype is anhedonia, the loss of pleasure or lack o...
...

Biological Computers: in Vivo Autonomous Doctors that Diagnose and Treat Disease

Summary Background: The promise of computers made from biological molecules lies in their potential to operate within the biochemical environment of a living organism and to interact with that environment through inputs and outputs with other biological molecules. For example, a biomolecular compute...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Summary Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow thr...
...

Next Generation Diagnostics for Breast Cancer, Prostate Cancer, and Melanoma

Summary From the labs of Professor Alun Jones (inventor of CardioliteTM) and Professor Ashfaq Mahmood, comes a series of small molecule imaging probes, capable of conjugation to both SPECT-based and PET-based imaging agents. Lead candidates include sigma-1 and selective sigma-2 receptor probes in th...
...

Genetic Test for Periodontal Disease

Summary Peridontitis is a serious oral disease characterized by highly painful loss of tissue between the tooth and the gingival tissue. In the US, it is estimated that dentists perform upwards of 28.5 million periodontal procedures each year, accounting for a multibillion dollar treatment market. ...
...

Detection of Invasive Bladder Cancer by DNA Hypermethylation

Summary Background: The National Cancer Institute has estimated that in year 2008, 68,810 new cases of bladder cancer will be diagnosed in United States, causing 14,100 deaths. The mortality of this cancer is attributed to the invasiveness of solid tumors into the muscular layers of the bladder. Cli...
...

Novel Biomarkers for Chronic Myelogenous Leukemia (CML)

Summary A high-dimensional metabolomics screen to diagnose and monitor chronic myeloid leukemia (CML) has been developed. Over 3000 metabolites are detected to yield a ‘cytogenetic signature’ that confirms the presence of cells bearing the Philadelphia chromosome, a genetic marker for CML. Throu...
...

C16:1n7-palmitoleate: a novel lipid hormone as a diagnostic and therapeutic tool

Summary Markets / Needs Addressed: Insulin resistance is the key factor of a collection of metabolic disorders including Type II diabetes, atherosclerosis, fatty liver disease, hypertension and dyslipidemia. The syndrome has reached epidemic levels worldwide, with 115 million individuals in 2004 suf...
...

Multiplexing long DNA syntheses

Summary The invention enables multiplex gene synthesis in a single reaction, using very low concentrations of oligonucleotides. The method, which employs a unique temporary tagging strategy, offers significant advantages in terms of speed and economy over existing methods for building genes, and eve...
...

A microfluidic differential manometer for cellular-scale hydrodynamics

Summary Background: The development and application of microfluidic systems have increased dramatically in recent years. The systems that typically involve controlled fluid flow through a number of microfluidic channels can provide unique platforms useful in both research and production. Flow thr...
...

Identification of Contactins and L1-CAMs as ligands for the Amyloid Precursor Protein

Summary Approximately 24 million people worldwide have dementia of which the majority (~60%) is due to Alzheimer’s; by 2040, it is projected that this figure will have increased to 81 million. The drugs currently on the market, such as cholinesterase inhibitors, reduce the cognitive symptoms of Al...
...

Mediators of Chronic Allograft Rejection and DNA Molecules Encoding Them

Summary This invention provides methods of detecting and treating arterial inflammatory disease, including chronic allograft rejection subsequent to organ transplantation or chronic vascular Chlamydia pnuemonii infection, that results in atherosclerosis or restenosis. A major limiting factor for ...
...

NF-X1: Novel Target for Inhibition of MHC Class II Transcription and Autoimmune Disease

Summary The class II MHC molecules function in the presentation of processed peptides to helper T cells during an immune response. Dysregulated expression of class II major histocompatibility complex (MHC) molecules is hypothesized to contribute to the pathogenesis of a severe combined immunodeficie...
...

Eotaxin: an Eosinophil Chemoattractant

Summary Investigators at Harvard Medical School have isolated and functionally characterized eotaxin, a novel mammalian chemokine that regulates migration of eosinophils, a class of leukocytes known to travel up chemokine concentration gradients created by the expression of well-characterized, gener...
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A Strain of the Ehrlichial Agent

Summary The NCH-1 strain of the agent of human granulocytic ehrlichiosis (HGE), isolated from a Nantucket patient in 1994, is available as a continuous culture in HL60 promyelocytic leukemia cells. This strain has been molecularly characterised, and is identical within the rDNA sequence that was ana...
...

Surface-Bound Double-Stranded DNA Protein Arrays

Summary Developed at Harvard Medical School, this invention provides nucleic acid arrays specifically designed for the assay of DNA-dependent protein:protein binding and/or physical interactions between proteins and nucleic acid molecules. Intermolecular analyses enabled by this technology are advan...
...

Identification of a Sequence Element from p53 that Signals for Mdm2-targeted Deg

Summary Research at Harvard University has led to a novel means of protecting the cell against oncogenic tumor formation. This invention provides compositions and methods for inhibiting degradation of tumor suppressor protein p53, thereby enhancing p53-mediated growth-suppressive properties. This te...
...

Single-Cell Transcriptional Analysis with Microarrays

Summary The enormous tissue and cellular diversity of multicellular organisms poses unique challenges, such as learning the gene expression patterns associated with embryonic or neurological development. One tool scientists use to help address these problems is DNA microarrays, which help provide me...
...

Aminoglycosides Scaffolding Targeting Antibiotic-Resistant Bacteria

Summary This invention encompasses key modifications to the chemistry of aminoglycosides to overcome bacterial resistance. The novel chemistry leverages: (a) the weak stereospecificity of aminoglycoside binding to rRNA, and (b) the stereospecific binding of extrinsic enzymes shown to inactivate amin...
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Targeting FtsZ Activity Using Novel Antibacterials: Screening Assays, Small Molecule Hits, and Synthetic Pathways to Potent Natural Products

Summary Inhibition of bacterial septation machinery represents a promising approach for countering antibiotic-resistant bacteria. One such approach targets FtsZ, an essential protein and key mediator of bacterial cell division, which is highly conserved in prokaryotes, yet absent in the mitochondria...
...

A Novel Class of Phospholipase A2 Preferentially Expressed in Th2 Cells

Summary This invention describes the identification of a novel, low molecular weight class of Phospholipase A2 enzyme, PLA2 Group XII, that is involved in Type 2 T helper (Th2) cell differentiation and activity. This enzyme provides a potential target for therapeutic modification of Th2 activity, in...
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Time and concentration warping software for RNA and protein expression data

Summary The predominant strategies for drug discovery today employ high-throughput screens and microarray-based methods that generate large data sets. Increasingly, these techniques are being used to study biological processes as a function of time and/or chemical concentration. Functionally-related...
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A Drug Discovery Platform Using Novel Labeled Uridine Analogs: Unique moieties contribute to molecular biology research

Summary Background: Uracil is a nucleic acid base that, when bound to ribose or deoxyribose, forms uridine or deoxyuridine, respectively. Certain analogs of uridine and deoxyuridine have been synthesized and used in a variety of applications. For example, deoxyribouridine analogs containing a 5-amin...
...

A Versatile Method for Accelerated Chromosome Walking and DNA Fingerprinting

Summary Background: A variety of DNA amplification methods deal with sequence analysis of an uncharacterized region adjacent to a known element. These methods include inverse PCR (iPCR), panhandle PCR, cassette ligation-anchored, and Thermal Asymmetric Interlaced PCR. Despite various degrees of succ...
...

Microcapillary Column Peptide Trap

Summary Protein detection using liquid chromatography (LC) followed by tandem mass spectroscopy has become a valuable tool for large-scale proteome analysis. Many efforts have been made to enhance throughput while maintaining sensitivity, particularly in the realm of low-volume samples. One such eff...
...

TreeDock protein docking software

Summary Predicting protein-protein and protein-ligand docking remains one of the challenging topics of structural biology. The main problems are (i) to reliably estimate the binding free energies of docked states, (ii) to enumerate possible docking orientations at a high resolution, and (iii) to con...
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HDAC Inhibitors

HDAC Inhibitors Laura Brass Email: laura_brass@harvard.edu Telephone: (617) 495-3067...
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Absolute Quantification of Proteins (AQUA)

Summary This invention provides methods, reagents and kits for obtaining absolute quantification of proteins and their modifications directly from cell lysates. In particular, the invention provides peptide internal standards for use in high performance liquid chromatography (HPLC) with online detec...
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Algorithm for Haplotype Construction of Single Nucleotide Polymorphism

Summary The Haplotyper software program utilizes a novel algorithm called Partition-Ligation to reconstruct individual haplotype phase information from unphased genoptype data. The Partition-Ligation method uses an ingenious Markov chain Monte Carlo approach both to construct the partial haplotypes ...
...

Tome-1, a novel cell-cycle gene required for mitotic entry

Summary This invention, out of Marc Kirschner's research group, concerns the discovery of a novel protein required for mititic entry. Harvard researchers have isolated and identified the protein and the gene encoding the protein. Further work has shown Tome-1's key role in the activation of cdk1/Cyc...
...

Saframycin Assay

Summary Background: Chemotherapeutic DNA-binding compounds exert their effects, in part, by preventing DNA synthesis and initiating events that leads to apoptotic death. Despite the excellent antiproliferative activity of these compounds, though, elucidating their particular modes of action (i.e. th...
...

Susceptibility to Tuberculosis 1 (sst1): Novel Target for Diagnosis and Treatment of Mycobacterium tuberculosis

Summary Background: Tuberculosis is the seventh most important cause of global premature mortality and disability. Currently there are 8 million new cases and 3 million deaths annually from tuberculosis, and it is projected that a total of 225 million new cases and 79 million deaths will occur betwe...
...

Multiplexing long DNA syntheses

Summary The invention enables multiplex gene synthesis in a single reaction, using very low concentrations of oligonucleotides. The method, which employs a unique temporary tagging strategy, offers significant advantages in terms of speed and economy over existing methods for building genes, and eve...
...

Bead-based polymerase colony microarrays and nucleic acid sequencing ("Polony-FISSEQ Beads")

Summary Foundational work of Dr. George Church has enabled the production and use of replicable arrays of polymerase-amplified nucleic acid colonies (so-called "polonies") on semisolid support matrices (see Harvard Case No. 1438). The present invention extends the polony technology to provided minia...
...

Novel Candidates for Tuberculosis Therapy

Summary There is a need for new antibiotics that are useful against tuberculosis. Proteasomes of Mycobacterium tuberculosium are essential in enabling these bacteria to evade killing by macrophages of the infected host. Proteasome inhibitors, an emerging class of drugs, enhance the ability of host m...
...

Amplification of trace amounts of nucleic acids

Summary The importance of nucleic acid amplification from trace amounts of biological sample has increased with the expansion of genomic sequencing in various clinical and research-oriented applications. This invention characterizes techniques to reduce background signal experienced while cloning su...
...

Genetic Test for Periodontal Disease

Summary Peridontitis is a serious oral disease characterized by highly painful loss of tissue between the tooth and the gingival tissue. In the US, it is estimated that dentists perform upwards of 28.5 million periodontal procedures each year, accounting for a multibillion dollar treatment market. ...
...

Targeting FtsZ Activity Using Novel Antibacterials: Screening Assays, Small Molecule Hits, and Synthetic Pathways to Potent Natural Products

Summary The emergence of bacterial strains resistant to current drugs has prompted a renewed effort to discover new methods for fighting infectious disease. A promising new target is FtsZ, a key mediator of bacterial cell division that is highly conserved among bacteria. Because FtsZ, much like its ...
...

Enhanced Nanoparticle-Based Molecular Imaging via MRI

Summary Enhanced Nanoparticle-Based Molecular Imaging via MRI Elevator pitch: For biotech and pharmaceutical companies who need to track drug effectiveness in the drug discovery and for MRI facilities, hospitals and physicians who seek to diagnose and treat specific diseases through molecular im...
...

palmitoleate: a novel lipid hormone as a diagnostic and therapeutic tool

Summary Markets / Needs Addressed: Insulin resistance is the key factor of a collection of metabolic disorders including Type II diabetes, atherosclerosis, fatty liver disease, hypertension and dyslipidemia. The syndrome has reached epidemic levels worldwide, with 115 million individuals in 2004 suf...
...

A Microfluidic Device and Methodology for Rapid, Quantitative Determination of Analyte Concentration in a Fluid Sample

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications, especially for biosensors and environmental monitoring. One recent area of interest is point-of-care diagnostic devices. These devices provide fast, accurate, and inexpensive sample analys...
...

Method of Detecting, Characterizing, and Quantifying Bulk, Light-Element Materials Using High-Energy, Penetrating X-Rays

Introduction X-rays have long been used in the characterization of a wide variety of materials. However, conventional techniques for dealing with light-element (or low-Z) materials, such as those containing nitrogen and carbon, are too low in energy to penetrate significant distances into enclosing ...
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Biomarkers for Cardiovascular Disease

Introduction Cardiovascular disease (CVD) is the leading cause of death among both men and women in the United States and, as such, is associated with high costs in terms of health care, morbidity, and mortality. An important aspect of the fight to reduce CVD is the development of diagnostic tests t...
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Programmable Generation and Transport of Droplets on Microscale Surface Texture Ratchets

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications. Currently, many laboratory processes can be combined on a single chip commonly only millimeters in size while manipulating liquid volumes of picoliter size or less. In order to effectively...
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Advanced Biomimetic Surfaces for Surface-Enhanced Raman Spectroscopy (SERS)

Introduction Raman spectroscopy characterizes molecules by their Raman scattering. As incoming light scatters off a molecule, most does so elastically, neither gaining nor losing energy, and this is known as Rayleigh scattering. Some of this light, however, about 1 in 10 million photons, is the subj...
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Respiratory Detection System (RDS)

Introduction Respiration rates are an important indicator of cellular and metabolic states in cells ranging from mammalian to bacterial, from individuals to large populations. Unfortunately, conventional techniques have significant drawbacks: for instance, electrochemical techniques have low sensiti...
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Biomarker for Caries Risk Assessment

Introduction Although dental caries (tooth decay) is largely preventable, it remains the most common chronic disease of children aged 5 to 17 years. According to the CDC, it is five times more common than asthma. Extensive research indicates that dental caries is the result of a bacterial infection ...
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Non-toxic protocol to establish paraoxonase 1 (PON1) status

Introduction Paraoxonase 1 (PON1) is a member of a three-gene family localized on human chromosome 7. Due to its role in the metabolism of both lipids and organophosphorus (OP) compounds, genetic variability in PON1 has been examined by many research groups as a risk factor for vascular and other d...
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Automated Genetic Approaches in Yeast to Identify Novel Drug Targets for Alzheimer’s Disease and Parkinson’s Disease

Introduction Alzheimer’s disease and Parkinson’s disease are the two most common neurodegenerative disorders in the world, affecting at least 11 million people in developed countries. To date there are very few drugs on the market for these devastating disorders, and the drugs that are available pro...
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Signal Amplification Method for SPR-based Chemical Detection

Introduction Surface plasma resonance (SPR) is a general spectroscopic method for sensing refractive index changes near the surface of a metal film. Its sensitivity to these changes provides a versatile platform for the observation and quantitation of chemical reactions and intermolecular binding at...
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Identification of Genes Involved in Cell Viability

Introduction Genes involved in cell viability are targets for diagnostic, prognostic, and therapeutic products related to aging. This technology encompasses methods, reagents, and pharmacological agents useful for extending life spans. Technology description Chronological lifespan in yeast has been ...
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Instrumentation and Optical Design for a High-Performance SPR Imager

Introduction Surface Plasmon Resonance (SPR) microscopy is a method to detect an analyze particles. SPR sensors work by shining a polarized light beam at an internally reflecting surface within a piece of glass which is coated with a metal on the outside, typically gold is used. This creates two opt...
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Chromosome 1 gene and gene products related to Alzheimer's disease

Introduction Alzheimer's disease (AD) is a devastating, neurodegenerative progressive disorder and is a common disease in the elderly and is the predominant cause of dementia in people over 65 years of age. The prevalence of AD is estimated to be as high as 18.7% and 47.2% for the 75-84 year and >=8...
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G-protein Coupled Glutamate Receptors

Introduction Glutamate plays a major, widespread role in the control of neuroendocrine neurons, possibly controlling not only the neuroendocrine system but other hypothalamic regions as well. There are four major subclasses of glutamate receptor, one of which is coupled to a membrane-bound, GTP-bind...
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Platelet Clotting Force Array

Introduction Advances in microfluidic technology are constantly opening doors to new and exciting applications, such as point-of-care diagnostic devices. These microscale diagnostic platforms will enable faster, cheaper, and more accurate patient evaluation for a wide array of parameters. One such p...
...

Clutter Rejection Filters for Doppler Optical Coherence Tomography

Introduction Doppler optical coherence tomography (DOCT) is a recently developed technology that can image blood flow with high special and temporal resolution. Technology description Researchers at the UW have developed techniques to improve the sensitivity of DOCT for real-time flow imaging by usi...
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Microfluidic Device and Method for Detection and Separation of Particles by Isoelectric Focusing

Introduction Electrophoresis and isoelectric focusing techniques are well suited to rapid isolation and detection of biological particles. Microfluidic devices are particularly amenable to electrophoresis-based applications, offering advantages such as low reagent and sample consumption, reduced ene...
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Near-Infrared Spectroscopic Instrument for Non-Invasive Clinical Chemistry

Introduction The analysis of biological samples is essential during patient care. Many such samples are traditionally measured using lengthy and complicated procedures which require the preconditioning of a blood sample withdrawn from the body. Preconditioning can be a lengthy and expensive process ...
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Exciplex™a Highly Sensitive Nucleic Acid Fluorescent Detection System

BACKGROUND Conventional fluorescent dyes used in nucleic acid detection methods suffer from a number of limitations. High backgrounds result in false positives and a reduction in sensitivity. The small Stokes shift (difference between excitation and emission wavelengths) often requires optical filte...
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Display of High Dynamic Range Images on Conventional Displays

The Invention Current image acquisition and processing allow construction of images with a very wide range of luminous levels. The invention is a method for intelligently manipulating the luminous levels into a displayed image where a maximum amount of significant viewable detail can be simultaneo...
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Non-invasive Diagnostics for Liver Diseases

The Invention A new serum marker specific for liver disease has been discovered. The marker is the soluble form of the human asialoglycoprotein receptor (ASGPR). An ELISA assay was developed with specific monoclonal antibodies that confirmed the correlation between reduced soluble ASGPR levels an...
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Early detection of cancer: Alu sequence exonization

The Invention A method has been developed whereby the generation of new Alu exons in particular genes may be used as diagnostic markers for different cancers. The method associates RNA splicing isoforms with specific cancers which may be readily detected by standard methods. The Need If detec...
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Novel Simple Assay for Diagnostics

The Invention A novel, simple and cost effective assay for monitoring blood levels of rapamycin (sirolimus), and rapamycin analogues was developed. The conceptual basis of the invention is based on the utilization of the Rapamycin specific target, its binding protein and their complex formation ...
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