Cell Lines that Express SIV/HIV Receptors and Co-Receptors in the K562 Human Cell Line

Both simian and human immunodeficiency viruses (SIV and HIV) utilize chemokine receptors, with or without CD4, as portals for entry into susceptible cells. JHU researchers constructed a reporter cell line derived from non-susceptible human K562 cells, xKLuSIV, that can be used to readily identify cells that are infected through a straightforward, quantitative luciferase assay. This cell line was further modified to express the rhesus macaque CD4 and/or the rhesus chemokine receptor CCR5 proteins. xKLuSIV cells that express CD4, with or without CCR5, are susceptible to infection with the macrophage-tropic, neurovirulent strain SIV/17E-Fr, but only xKLuSIV cells expressing both CCR5 and CD4 were susceptible to injection by the macrophage-tropic, non-neurovirulent strain SIV/17E-Cl. Description (Set) Proposed Use (Set) These cell lines provide a unique resource to look at entry of macrophage-tropic strains of HIV and SIV.

Inventor(s): Clements, Janice E.

Type of Offer: Licensing

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