Multiple Inversion Flip Flop (MIFF) Vectors

JHU researchers have developed novel Multiple Inversion Flip Flop (MIFF) plasmid vectors for controlling the expression of transgenes that are either stably-integrated or transient. These vectors comprise a cytomegalovirus (CMV) promoter flanked by LoxP sites in opposing orientations, which allow for reversible inversion of the promoter in the presence of the Cre recombinase, and the ability to turn gene expression on and off. The vectors also incorporate reporter transgenes encoding fluorescent proteins or drug selectable markers for monitoring inversion and selection. The MIFF system has several notable advantages over current transgene regulation systems, including reversibility, non-detectable background transgene expression, high levels of stable transgene expression, and efficient selection of stable clones. In addition, the MIFF system removes the need for continual administration of an inducer or repressor agent for the control of transgene expression, as with common inducible genetic systems. Description (Set) Proposed Use (Set) The MIFF expression system provides an important tool for studying gene function and for generating models to study the genetic basis of disease, where the tight regulation of gene expression is very important.

Inventor(s): Park, Ben Ho

Type of Offer: Licensing

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