Selective Fluorescent Labeling of S-Nitrosothiols: S-FLOS

Johns Hopkins University is seeking licensees for a powerful analytical and quantitative technique for S-nitrosylation of proteins. S-nitrosylation is a post-translational protein modification that can alter protein structure and activity. Emerging evidence suggests that dysregulation of S-nitrosylation is related to the pathophysiology of many disease processes. To date, multiple proteins associated with neurodegenerative disease were found to be S-nitrosylated. Identified S-nitrosylated proteins from neuronal tissue are potential therapeutic targets for neurodegenerative diseases. Detection of S-nitrosylation in proteins is a recognized challenge. JHU scientists have developed a simple, sensitive detection method that uses fluorescent dyes.
• Non-biological fluorescent tag produces low background noise, with drastically reduced false positive results to allow data collection with greater sensitivity.
• Use of fluorescent dyes to label proteins without signaling enrichment steps produces quantifiable data in less time.
• Two fluorescent dye system allows direct comparison of two labeled protein samples on the same substrate to provide quantitative, spatial and temporal information on S-nitrosylation and normal cell signaling.
• Detection of relative changes in S-nitrosothiol content of individual proteins in complex mixtures broadens experimental design potential. Proposed Use (Set) This technology can be commercialized as a detection kit for quantifying changes in protein nitrosylation. It can also be used as a method to identify agents that modulate protein S-nitrosylation useful for therapeutic drug development. Patent (Set) Int'l Pub. NO. WO 2008/036328

Inventor(s): Cole, Robert

Type of Offer: Licensing

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