Film Immobilized Capture-Particles
INVENTION: A new method of performing and analyzing protein-protein interactions, immuno-hybridization, and nucleic hybridization has been developed. This novel method utilizes capture particles immobilized on a thin film coated on a planar substrate. The particles can be arranged on the substrate as an array, and a variety of capture particles can be placed onto a single substrate. The analyte of interest is incubated with the array, and an optical detection system is subsequently used to quantify analyte binding to the capture particle on the film surface and to locate the particles attached to the film.
DESCRIPTION This invention has several advantages over existing approaches. The solid phases and coating conditions for the planar substrate can be optimized for each different capture reagent. Particles are commercially available with numerous surfaces for passive adsorption or covalent coupling to the film. Furthermore, reagents can be attached to the capture particles in a wide range of buffers with various pHs and ionic strengths, which can improve the density, kinetic rate constant, and equilibrium affinity constant of the capture reagent. Non-specific binding can also be reduced, as the capture reagents are attached to the particles with minimal changes to their binding sites, and the surface of the array can be overcoated with blockers. U.S. Patent No. 7,172,804
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