Optimization of Expression Construct and Purification Protocols for a MSP1.42 Malaria Vaccine

Description The invention consists of two parts which both contribute to an enhanced feasibility of commercial production of this potential malaria vaccine: (1) optimization of the MSP1.42 gene construct for enhanced levels of production in the baculovirus/insect cell culture expression system, and (2) development of a simplified, non-denaturing purification scheme which enhances recovery and purity of the MSP1.42 polypeptide.

Applications Malaria vaccine

Main Advantages Approximately 5-10 fold increases in the level of expression of the recombinant polypeptide were achieved for insect cell cultures infected with recombinant baculovirus containing the optimized MSP1.42 construct. The simplified purification scheme utilizes commercially available column matrixes to purify MSP1.42 to homogeneity and at high yield in a few steps.

Type of Offer: Licensing

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