Serotype-Specific Probes and Nucleotide Sequences of Listeria Monocytogenes Cluster IIB (4b-4d-4e)

Description Two genes which are unique to serotype cluster IIB (4b - 4d - 4e) have been characterized for Listeria monocytogenes. Serotype 4b is known to be the primary pathogenic species causing listeriosis. The serotype-specific region has been sequenced in its entirety from both strands. The sequences themselves correspond to novel genes, which are involved in the expression of unique surface antigens of L. monocytogens cluster IIB. In tests of 150 strains of Listeria, the unique sequence could detect any occurence of the 4b serotype. Applications The genes can be used in Southern and DNA dot blots for specific detection and primers have been designed from the genes to be used for sensitive and unambiguous detection and typing using Polymerase Chain Reaction (PCR). Main Advantages The sequences are the first serotype-specific sequences that can detect strains of certain serotype-specific groups and is the first demonstration of a PCR-based approach for detection of certain serotypic groups in this pathogen.

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