A Novel Vectors for Enhanced Production of Double-Stranded RNA for RNA-Mediated Gene Interference
Double stranded RNA (dsRNA) can specifically inhibit gene function by eliminating gene expression (RNA mediated gene interference, RNAi). Introduction of dsRNA for RNAi can be accomplished by feeding bacteria engineered to produce these dsRNA molecules. This invention describes engineered vectors that effectively produce dsRNA in bacteria. An interesting aspect of the invention is the addition of terminator sequences to the vectors, which enhance inhibition of gene expression by dsRNA.
These vectors can be used to inhibit gene expression in C. elegans and planarians. The bacteria expressing the dsRNA from these vectors are incorporated in planarians through their food. This methodology could have therapeutic applications if extended to parasites.
Stage of Development
A PCT patent application has been filed with the U.S. Patent and Trademark Office and is pending. World Intellectual Property Organization Publication number: WO2005047300A2 and US publication number: US 2007/0020652 A1.
This technology is part of an active and ongoing research program/has been demonstrated to work in proof-of-concept experiments. It is available for developmental research support/licensing under either exclusive or non-exclusive terms.
*Reddien PW, Bermange AL, Murfitt KJ, Jennings JR and Alvarado AS (2005) Identification of Genes Needed for Regeneration, Stem Cell Function and Tissue Homeostais by Systematic Gene Perturbation in Planaria. Develp Cell, 8, 635-649.
*Dr. Alvarado� s Website: http://planaria.neuro.utah.edu/
Alejandro Sanchez Alvarado, Peter Reddien, Adam Bermange
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