Method for Detecting a Circularized DNAa, and Use of Said Method for Detecting Mutations
The present invention relates to a method for detecting a circularized single-stranded DNA by means of the isothermal hyperbranched rolling circle amplification technique, in which the primers used comprise a detectable barcode sequence and, optionally, a spacer which blocks polymerization by DNA polymerase. The present invention also relates to the use of said method for detecting a genetic polymorphism of one or more base pair(s).
WO 2,011,018,774 [MORE INFO
BOCKELMANN ULRICH [FR]; VIASNOFF VIRGILE [FR]; CISSE ISMAIL [FR]
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