Primers, Probes and Methods for Nucleic Acid Amplification
Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit "single-tube" processing.
WANGH LAWRENCE [US]; RICE JOHN [US]; SANCHEZ AQUILES [US]; PIERCE KENNETH [US]; SALK JESSE [US]; REIS ARTHUR [US]; HARTSHORN CRI
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