Novel Oxalate/Formate Exchange Protein

A DNA sequence encoding a transport protein responsible for the physical movement of oxalate and formate across the plasma membrane is provided. The protein, OxlT, can be isolated from the gram-negative anaerobe, Oxalobacter formigenes. This protein is part of the three-protein oxalate/formate antiport exchange system that allows for conversion of extracellular oxalate to formate + CO2. The OxlT coding sequence can be used to provide a metabolic energy system to the cell. The system comprises the OxlT transport protein in combination with oxalylCoA decarboxylase and formyl-CoA transferase. Expression cassettes can be designed to express all or part of the system in a target host. Such energy generating systems can be used in industrial settings that utilize fermentation. These energy generating systems extend culture longevity and increase product yield. Description (Set) Proposed Use (Set) The Ox1T sequence in construction of a plasmid vector to providing energy (ATP) to bacterial cells in the fermentation and biotechnology industries. It is a anticipated that this plasmid will considerably extend the lifetime of production runs, allowing greater yields of biosynthetic products.

Patent (Set) 6,162,632

Patents:
US 6,162,632

Inventor(s): Abe, Keietsu

Type of Offer: Licensing



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