Electroporation System for Enhancing Wound Healing Using Plasmid DNA Expression Vectors

We have invented a gene therapy protocol (system, kit) that enhances wound healing. Our approach is to combine the application of plasmids expressing growth factors and the technique of electroporation to enhance transfection efficiency of the plasmid transfection. The combination of these two approaches is the basis of the invention. Various growth factors have the potential to enhance cutaneous wound healing. Promising growth factors are Kertinocyte Growth Factor-1 (KGF-1). Platelet Derived Growth Factor (PDGF), and Vascular Endothelial Growth Factor (VEGF). Unfortunately, when applied topically to cutaneous wounds, they are rapidly deactivated by proteases within and surrounding the wound tissue. We have developed a plasmid mediated gene transfer protocol to deliver the DNA for growth factors into the wound where they are expressed continuously within the wound tissue and where we have demonstrated that they improve wound healing. We have also shown the electroporation improves transfection efficiency. The combination of these two approaches is our invention. Description (Set) Proposed Use (Set) This gene therapy system could be used to enhance wound healing in numerous clinical situations. For cutaneous wound healing it could enhance the healing of venous ulcers, decubitus ulcers, diabetic foot ulcers, among other lesions. In the gastrointestinal tract this type of therapy could potentially be delivered endoscopically to assist in the healing of gastric and duodenal ulcers. In the area of anal rectal surgery this kind of therapy could be used to heal anal fissures. Patent (Set) WO 04/060476

Inventor(s): Harmon, John W.

Type of Offer: Licensing

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