A Novel Method for Generating and Screening Peptides and Libraries Displayed on Adenovirus Fiber

Abstract (Set) Capsid-displayed adenoviral peptide libraries have been a significant yet unfeasible goal in biotechnology. Three barriers have made this difficult: the large size of the viral genome, the low efficiency of converting plasmid-based genomes into packaged adenovirus, and the fact that adenoviral library amplification is hampered by the ability of two (or more) virus to co-infect one cell. JHU scientists developed a novel method, which is capable of overcoming all three barriers. First, modified Fiber genes can be shuttled from small, manageable sized plasmids into the natural genetic locus of the large adenovirus genome. Secondly, modified Fiber genes can be directly shuttled into replicating viral genomes in mammalian cells. Finally, successful Fiber genes can be rescued from virus and recombined back into shuttle plasmids, avoiding the need to propagate mixed viral pools. Combined, these methodologies allow one to generate and screen modified-fiber gene libraries through iterative rounds of selection for infection of a cell or tissue of choice. Description (Set) Proposed Use (Set) This technology allows to generate and screen modified capsid gene libraries in adenovirus.

Inventor(s): Lupold, Shawn E. ,Chowdhury, Wasim Haider,Kudrolli, Tarana,Rodriguez, Ronald

Type of Offer: Licensing

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