Direct Reading, Quantitative Biosensors for Measuring Chemical Concentrations
Bioluminescence and chemiluminescence are chemical oxidation processes that result in photon emission. Adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NADH) are two molecules that play unique and central roles in biology and are the basic coupling agents of cellular metabolism. Many biochemical enzyme processes involve one of these two molecules. Evolution has produced bioluminescence processes based on these two molecules. This invention describes diagnostic and detection devices and methods that use an analyte-specific luminescent signal where the spatial position of the luminescent signal is related to the analyte concentration.Benefits
This technology can be applied to a sensitive, quantitative, luminescence-based biosensor that reads analyte concentration via spatial distribution of emitted light. The detection of light is used to assess the spatial position, rather than the intensity or wavelength, of emitted light. A bio-luminescent or chemi-luminescent reaction requiring, for example, ATP, NADPH or NADH as a specific and sensitive co-factor is used. The operator using the sensor can detect the spatial position of a light source in a reproducible and quantitative manner. This technology can be used in the manufacture of specific, quantitative, rapid, direct reading, inexpensive, and disposable analytical devices.
Stage of Development
A patent (US 6,501,549 B1) has been issued from US Patent and Trademark Office.
This technology is seeking partners for development-manufacturing-sales-etc of the working-final-etc product. It is available for developmental research support/licensing under either exclusive or non-exclusive terms.
Additional Info
*C. Y. Wang, S. Hitz, J. D. Andrade, R. J. Stewart. Specific immobilization of Firelfy Luciferase through a biotin carboxyl carrier protein domain. Anal. Biochem. 1997 Mar 1; 246(1):133-9.
*http://www.bioen.utah.edu/faculty/JDA/_vti_cnf/Controls/main_view.html
Inventor(s): Chung-Yih Wang, Vladimir Hlady, Joseph Andrade
Type of Offer: Licensing
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