Outer Membrane Protein B1 of Moraxella Catarrhalis
The present invention includes a method for the isolation and purification of outer membrane protein B1 as well as B1 peptides from Moraxella catarrhalis, and the resulting compositions. The method involves growing the bacteria in iron-depleted medi um to enhance the expression of the B1 protein, harvesting the bacteria from the culture, extracting from the harvested bacteria a preparation substantially comprising an outer membrane preparation with an affinity matrix containing immobilized trans ferring wherein B1 protein binds to the transferring, and eluting the bound B1 protein from the transferring. Applications for the purified B1 protein include but are not limited to use as an immunogen for vaccine formulations and as antigens in dia gnostic immunoassays.
Patents Issues: 6,004,562
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